Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 744 338.8 17650688 97.8% 17262372 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,091,351 C→T G168D (GGC→GAC)  rhaD ← rhamnulose‑phosphate aldolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,091,3510CT100.0% 776.7 / NA 221G168D (GGC→GAC) rhaDrhamnulose‑phosphate aldolase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (103/118);  total (103/118)

Only 100 of 239 total aligned reads displayed.

GCGGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGCCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGATC  >  NC_000913/4091261‑4091440
                                                                                          |                                                                                         
   gTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTcc                                                                                      >  1:1290768/1‑93 (MQ=255)
   gTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTcc                                                                                      >  2:7243807/1‑93 (MQ=255)
   gTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTcc                                                                                      >  1:2843144/1‑93 (MQ=255)
    tCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTccc                                                                                     >  1:8715692/1‑93 (MQ=255)
       ccTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCcaca                                                                                  >  2:3452046/1‑93 (MQ=255)
       ccTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCcaca                                                                                  >  2:4591834/1‑93 (MQ=255)
         tGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGt                                                                                <  1:5343916/93‑1 (MQ=255)
         tGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGt                                                                                >  2:749959/1‑93 (MQ=255)
         tGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGt                                                                                >  1:5449709/1‑93 (MQ=255)
         tGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGt                                                                                >  2:5284189/1‑93 (MQ=255)
         tGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGCGCTGTCTTCCCACAGt                                                                                >  2:8111549/1‑93 (MQ=255)
          ggCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATCCCACCAGACACACGGTGCTGTCTTCCCACAGtt                                                                               <  2:6699211/93‑1 (MQ=255)
          ggCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGtt                                                                               <  1:8344224/93‑1 (MQ=255)
           gCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTg                                                                              >  1:7259859/1‑93 (MQ=255)
            ccGATTTCGTCCGTGCCGGGCACCATCCAGGGCAAAATGCCAACGCCATCCGGGAATACCCCCAGACACTCGGTGCTGTCTTCCCACAGTTgg                                                                             <  1:7053862/93‑1 (MQ=255)
            ccGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTgg                                                                             <  1:6575547/93‑1 (MQ=255)
            ccGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTgg                                                                             >  1:1466366/1‑93 (MQ=255)
              gATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCg                                                                           <  1:3942583/93‑1 (MQ=255)
                  tCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTg                                                                       <  2:3178505/93‑1 (MQ=255)
                    gTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACTCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGaa                                                                     <  1:5286664/93‑1 (MQ=255)
                    gTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGaa                                                                     <  2:199934/93‑1 (MQ=255)
                    gTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGaa                                                                     <  2:3719871/93‑1 (MQ=255)
                    gTCCGTGCCGCGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCCCCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGaa                                                                     <  2:3335784/93‑1 (MQ=255)
                     tCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAg                                                                    >  2:4691876/1‑93 (MQ=255)
                      ccGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGa                                                                   >  2:5788606/1‑93 (MQ=255)
                          gCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACcgc                                                               <  2:1378380/93‑1 (MQ=255)
                          gCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACcgc                                                               <  1:5199199/93‑1 (MQ=255)
                            cGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCgg                                                             >  2:6429718/1‑93 (MQ=255)
                              ggCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGgtg                                                           >  1:7984153/1‑93 (MQ=255)
                               gCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGgtgt                                                          <  2:7774237/93‑1 (MQ=255)
                                 aCCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCg                                                        >  1:4324964/1‑93 (MQ=255)
                                 aCCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCg                                                        <  1:2766745/93‑1 (MQ=255)
                                  ccATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGt                                                       >  2:5257190/1‑93 (MQ=255)
                                  ccATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGt                                                       >  2:2295610/1‑93 (MQ=255)
                                  ccATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGt                                                       <  2:6996972/93‑1 (MQ=255)
                                  ccATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGt                                                       <  2:8083045/93‑1 (MQ=255)
                                   cATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGtt                                                      >  1:614876/1‑93 (MQ=255)
                                   cATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGtt                                                      >  2:6179011/1‑93 (MQ=255)
                                    ctCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGttt                                                     <  2:5936880/92‑1 (MQ=255)
                                    aTCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGttt                                                     >  1:5931844/1‑93 (MQ=255)
                                    aTCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGttt                                                     >  1:806892/1‑93 (MQ=255)
                                     tCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGtttt                                                    >  1:1104699/1‑93 (MQ=255)
                                       cACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCa                                                  <  2:7350954/93‑1 (MQ=255)
                                          ggCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGt                                               >  2:1065286/1‑93 (MQ=255)
                                           gCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTa                                              >  1:6864103/1‑93 (MQ=255)
                                               aaTGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATa                                          <  1:800518/93‑1 (MQ=255)
                                               aaTGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATa                                          <  1:7547309/93‑1 (MQ=255)
                                               aaTGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATa                                          >  2:692183/1‑93 (MQ=255)
                                                aTGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAg                                         >  2:6274987/1‑93 (MQ=255)
                                                aTGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAg                                         <  2:2568463/93‑1 (MQ=255)
                                                  cccAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGt                                       <  2:6556451/92‑1 (MQ=255)
                                                    cAACGCCATCCGGGAATACCACCAGACGCTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGa                                     <  1:2150679/93‑1 (MQ=255)
                                                    cAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCGCAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGa                                     <  1:2931825/93‑1 (MQ=255)
                                                    cAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGa                                     <  2:2913353/93‑1 (MQ=255)
                                                    cAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGa                                     <  2:6580851/93‑1 (MQ=255)
                                                    cAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGa                                     <  1:6014050/93‑1 (MQ=255)
                                                    cAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGa                                     <  1:701323/93‑1 (MQ=255)
                                                     aaCGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAg                                    <  2:1832359/93‑1 (MQ=255)
                                                      aCGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAgg                                   <  2:503635/93‑1 (MQ=255)
                                                      aCGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAgg                                   <  2:4853354/93‑1 (MQ=255)
                                                      aCGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAgg                                   >  2:4798602/1‑93 (MQ=255)
                                                         ccgtCCGAGATTAACACCAGACACTAAGAGCTGTCTTCCCAAAGTAGGCAAGTAAAGACCGCGGTGACGTTTTAAAATACATAGGTGAGGGCg                                <  2:8059324/90‑1 (MQ=255)
                                                            tCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATc                             <  1:2621722/93‑1 (MQ=255)
                                                            tCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATc                             <  1:3135893/93‑1 (MQ=255)
                                                            tCCGGGAATACCACCAGACACGCGGGGCTGTCTTCCCTCAGTTGGCGAGTGAAGACCGCGGAGTCGGGGTCAAGTACATAGGTGAGGGCGATc                             >  2:7842500/1‑93 (MQ=255)
                                                              cGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAg                           <  2:7627671/93‑1 (MQ=255)
                                                              cGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAg                           <  2:7978425/93‑1 (MQ=255)
                                                              cGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAg                           <  2:5003180/93‑1 (MQ=255)
                                                               gggAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAgg                          >  1:7915556/1‑93 (MQ=255)
                                                               gggAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAgg                          <  1:1361296/93‑1 (MQ=255)
                                                                ggAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGt                         >  2:1935606/1‑93 (MQ=255)
                                                                 gAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGtt                        <  2:7030200/93‑1 (MQ=255)
                                                                  aaTACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTtg                       >  2:5100861/1‑93 (MQ=255)
                                                                  aaTACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTtg                       >  1:4897432/1‑93 (MQ=255)
                                                                  aaTACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTtg                       <  2:3491135/93‑1 (MQ=255)
                                                                   aTACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTtgg                      >  2:4011046/1‑93 (MQ=255)
                                                                    tACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTGGTTTTCAAGTACATAGGTGAGGGCGATCAGGTtggg                     >  1:2727616/1‑92 (MQ=255)
                                                                    tACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTtggt                     <  1:5748649/93‑1 (MQ=255)
                                                                      ccaccaGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTtggtgg                   <  2:2957929/93‑1 (MQ=255)
                                                                        cccaGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGgtggcg                 <  2:4237431/92‑1 (MQ=255)
                                                                        accaGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGgtggcg                 >  1:2528108/1‑93 (MQ=255)
                                                                        accaGACACTCAGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGgtggcg                 >  2:447430/1‑93 (MQ=255)
                                                                         ccaGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGgtggcgt                <  1:4858349/93‑1 (MQ=255)
                                                                         ccaGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGgtggcgg                >  1:293184/1‑92 (MQ=255)
                                                                          caGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGgtggcgtg               >  1:3238118/1‑93 (MQ=255)
                                                                           aGACTCTCGGTGATGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGgtggcgtgg              <  1:3750777/93‑1 (MQ=255)
                                                                            gACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGgtggcgtggc             <  2:8468804/93‑1 (MQ=255)
                                                                               acTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGt          >  2:8493100/1‑93 (MQ=255)
                                                                               acTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGt          <  1:4728676/93‑1 (MQ=255)
                                                                                cTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTg         >  1:3331142/1‑93 (MQ=255)
                                                                                  cGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCa       >  1:3664285/1‑93 (MQ=255)
                                                                                   ggTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCAt      <  2:1015719/93‑1 (MQ=255)
                                                                                    gTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATg     <  2:3300860/93‑1 (MQ=255)
                                                                                    gTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATg     >  1:1640274/1‑93 (MQ=255)
                                                                                    gTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATg     >  1:4586463/1‑93 (MQ=255)
                                                                                     tgctgtCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGa    <  1:8532944/93‑1 (MQ=255)
                                                                                      gctgtCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGAt   >  1:2082147/1‑93 (MQ=255)
                                                                                      gctgtCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGAt   <  1:7326241/93‑1 (MQ=255)
                                                                                      gctgtCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGAATGAt   >  1:6644419/1‑93 (MQ=255)
                                                                                       ctgtCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGATc  <  1:1041428/93‑1 (MQ=255)
                                                                                          |                                                                                         
GCGGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGCCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGATC  >  NC_000913/4091261‑4091440

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGCGGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGCCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGATCACCC  >  NC_000913/4091260‑4091444
                                                                                           |                                                                                             
TGCGGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCT                                                                                             <  SRR4302541.1954502/94‑1 (MQ=60)
 GCGGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTT                                                                                            >  SRR4302541.4614573/1‑94 (MQ=60)
   GGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCC                                                                                          >  SRR4302541.6319223/1‑94 (MQ=60)
   GGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCC                                                                                          >  SRR4302541.2871730/1‑94 (MQ=60)
   GGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCC                                                                                          >  SRR4302541.1303520/1‑94 (MQ=60)
    GTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCC                                                                                         <  SRR4302541.8264355/94‑1 (MQ=60)
    GTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCC                                                                                         >  SRR4302541.8808914/1‑94 (MQ=60)
     GCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCA                                                                                        <  SRR4302541.8092491/94‑1 (MQ=60)
      CGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCAC                                                                                       <  SRR4302541.277295/94‑1 (MQ=60)
      CGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCAC                                                                                       <  SRR4302541.1352288/94‑1 (MQ=60)
       GCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACA                                                                                      >  SRR4302541.3469397/1‑94 (MQ=60)
        CCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAG                                                                                     >  SRR4302541.3293349/1‑94 (MQ=60)
         CTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGT                                                                                    <  SRR4302541.6791706/94‑1 (MQ=60)
         CTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGT                                                                                    <  SRR4302541.1311596/94‑1 (MQ=60)
         CTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGT                                                                                    >  SRR4302541.5505627/1‑94 (MQ=60)
          TGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTT                                                                                   <  SRR4302541.5398860/94‑1 (MQ=60)
          TGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTT                                                                                   <  SRR4302541.3237308/94‑1 (MQ=60)
          TGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTT                                                                                   >  SRR4302541.4291495/1‑94 (MQ=60)
           GGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTG                                                                                  >  SRR4302541.7337306/1‑94 (MQ=60)
           GGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTG                                                                                  <  SRR4302541.8434803/94‑1 (MQ=60)
            GCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGG                                                                                 >  SRR4302541.1480727/1‑94 (MQ=60)
             CCGATTTCGTCCGTGGCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACGAACAGATACTCGGTGCTGTCTTCCCACAGTTcgg                                                                                >  SRR4302541.5429898/1‑91 (MQ=60)
             CCGATTTCGTCCGTGCCGGGCACCATCCAGGGCAAAATGCCAACGCCATCCGGGAATACCCCCAGACACTCGGTGCTGTCTTCCCACAGTTGGC                                                                                <  SRR4302541.7128885/94‑1 (MQ=60)
             CCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGC                                                                                <  SRR4302541.6642824/94‑1 (MQ=60)
              CGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCG                                                                               >  SRR4302541.7225423/1‑94 (MQ=60)
              CGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCG                                                                               >  SRR4302541.8118512/1‑94 (MQ=60)
               GATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGA                                                                              <  SRR4302541.3981804/94‑1 (MQ=60)
               GATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGA                                                                              >  SRR4302541.2809390/1‑94 (MQ=60)
               GATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGA                                                                              >  SRR4302541.8913363/1‑94 (MQ=60)
               GATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCAGTGCTGTCTTCCCACAGTTGGCGA                                                                              >  SRR4302541.6493605/1‑94 (MQ=60)
                  TTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTG                                                                           <  SRR4302541.2107395/94‑1 (MQ=60)
                   TCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGA                                                                          >  SRR4302541.4202250/1‑94 (MQ=60)
                    CGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAA                                                                         >  SRR4302541.2761523/1‑94 (MQ=60)
                    CGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAA                                                                         <  SRR4302541.5293993/94‑1 (MQ=60)
                    CGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAA                                                                         >  SRR4302541.7046866/1‑94 (MQ=60)
                     GTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACTCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAG                                                                        <  SRR4302541.5341090/94‑1 (MQ=60)
                        CGTCCCGTGCACCATCCACGGCAAAATGCCAACACCATCCGGTAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACC                                                                     <  SRR4302541.6745822/94‑1 (MQ=60)
                           GCCGGGCACCATCCACGGCTAAATGCCAACGCCATCCGGGAATACCACCAGACCCTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCG                                                                  <  SRR4302541.6856797/94‑1 (MQ=60)
                           GCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACCCTCGGTGCTGTCTGCCCACAGTTGGCGAGTGAAGACCGCG                                                                  <  SRR4302541.2353799/94‑1 (MQ=60)
                           GCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCG                                                                  >  SRR4302541.1108547/1‑94 (MQ=60)
                           GCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCG                                                                  <  SRR4302541.5252738/94‑1 (MQ=60)
                             CGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGT                                                                <  SRR4302541.2989326/94‑1 (MQ=60)
                              GGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTG                                                               >  SRR4302541.8072086/1‑94 (MQ=60)
                              GGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTG                                                               <  SRR4302541.1869200/94‑1 (MQ=60)
                                GCACCATCCACGGCAACATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTC                                                             <  SRR4302541.7873103/94‑1 (MQ=60)
                                 CACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCG                                                            >  SRR4302541.4368146/1‑94 (MQ=60)
                                  ACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGT                                                           <  SRR4302541.2794592/94‑1 (MQ=60)
                                  ACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGT                                                           >  SRR4302541.2375031/1‑94 (MQ=60)
                                  ACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGT                                                           >  SRR4302541.1274944/1‑94 (MQ=60)
                                   CCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTT                                                          >  SRR4302541.514829/1‑94 (MQ=60)
                                   CCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTT                                                          >  SRR4302541.620642/1‑94 (MQ=60)
                                    CATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTT                                                         <  SRR4302541.4786982/94‑1 (MQ=60)
                                    CATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTT                                                         >  SRR4302541.5992267/1‑94 (MQ=60)
                                    CATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTT                                                         >  SRR4302541.814294/1‑94 (MQ=60)
                                     ATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTT                                                        >  SRR4302541.1115054/1‑94 (MQ=60)
                                       CCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCA                                                      >  SRR4302541.7360986/1‑94 (MQ=60)
                                        CACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAA                                                     <  SRR4302541.4642564/94‑1 (MQ=60)
                                          CGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGT                                                   <  SRR4302541.8089369/94‑1 (MQ=60)
                                          CGGCAAAATGCCAACGCCATCCGGGAATAACACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGT                                                   <  SRR4302541.8622092/94‑1 (MQ=60)
                                           GGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTA                                                  >  SRR4302541.6937434/1‑94 (MQ=60)
                                              AAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACAT                                               <  SRR4302541.5455474/94‑1 (MQ=60)
                                              AAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACAT                                               >  SRR4302541.8654741/1‑94 (MQ=60)
                                                AATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAG                                             <  SRR4302541.7627967/94‑1 (MQ=60)
                                                AATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAG                                             >  SRR4302541.6667351/1‑94 (MQ=60)
                                                AATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAG                                             <  SRR4302541.807880/94‑1 (MQ=60)
                                                AATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAG                                             <  SRR4302541.798575/94‑1 (MQ=60)
                                                 ATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGG                                            <  SRR4302541.2909519/94‑1 (MQ=60)
                                                  TGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGT                                           >  SRR4302541.3783785/1‑94 (MQ=60)
                                                   GCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTG                                          >  SRR4302541.3368184/1‑94 (MQ=60)
                                                   GCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTG                                          <  SRR4302541.5382116/94‑1 (MQ=60)
                                                     CAACGCCATCCGGGAATACCACCAGACGCTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAG                                        <  SRR4302541.2171477/94‑1 (MQ=60)
                                                     CAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCGCAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAG                                        <  SRR4302541.2961305/94‑1 (MQ=60)
                                                     CAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCGCAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAG                                        <  SRR4302541.2961306/94‑1 (MQ=60)
                                                     CAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAG                                        >  SRR4302541.2628460/1‑94 (MQ=60)
                                                     CAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAG                                        <  SRR4302541.707917/94‑1 (MQ=60)
                                                     CAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAG                                        <  SRR4302541.6075680/94‑1 (MQ=60)
                                                        CGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGC                                     <  SRR4302541.1410770/94‑1 (MQ=60)
                                                         GCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCG                                    <  SRR4302541.7153666/94‑1 (MQ=60)
                                                             TCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCA                                <  SRR4302541.3167583/94‑1 (MQ=60)
                                                             TCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCA                                <  SRR4302541.2647889/94‑1 (MQ=60)
                                                              CCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAG                               >  SRR4302541.5463605/1‑94 (MQ=60)
                                                               CGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGG                              <  SRR4302541.271751/94‑1 (MQ=60)
                                                               CGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGG                              >  SRR4302541.8002669/1‑94 (MQ=60)
                                                                GGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGT                             <  SRR4302541.1374724/94‑1 (MQ=60)
                                                                GGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGT                             <  SRR4302541.3256664/94‑1 (MQ=60)
                                                                GGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGT                             <  SRR4302541.3857665/94‑1 (MQ=60)
                                                                  GAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTG                           <  SRR4302541.7476625/94‑1 (MQ=60)
                                                                  GAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTG                           >  SRR4302541.4947403/1‑94 (MQ=60)
                                                                  GAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTG                           <  SRR4302541.6167407/94‑1 (MQ=60)
                                                                  GAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTG                           >  SRR4302541.4947399/1‑94 (MQ=60)
                                                                  GAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTG                           <  SRR4302541.1649995/94‑1 (MQ=60)
                                                                    ATCCCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGT                         <  SRR4302541.4805342/94‑1 (MQ=60)
                                                                    ATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTGGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGG                         >  SRR4302541.2754889/1‑94 (MQ=60)
                                                                     TACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTG                        <  SRR4302541.5807186/94‑1 (MQ=60)
                                                                        CACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCG                     >  SRR4302541.2553525/1‑94 (MQ=60)
                                                                         ACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGG                    >  SRR4302541.295728/1‑94 (MQ=60)
                                                                          CCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTG                   <  SRR4302541.8837912/94‑1 (MQ=60)
                                                                          CCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTG                   >  SRR4302541.3270815/1‑94 (MQ=60)
                                                                          CCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTG                   <  SRR4302541.4907893/94‑1 (MQ=60)
                                                                           CAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGG                  >  SRR4302541.6056728/1‑94 (MQ=60)
                                                                            AGACTCTCGGTGATGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGC                 <  SRR4302541.3788512/94‑1 (MQ=60)
                                                                            AGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGC                 >  SRR4302541.5829060/1‑94 (MQ=60)
                                                                            AGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGC                 >  SRR4302541.8069006/1‑94 (MQ=60)
                                                                             GACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCA                >  SRR4302541.190183/1‑94 (MQ=60)
                                                                              ACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAG               <  SRR4302541.5969639/94‑1 (MQ=60)
                                                                               CACTCGGTGCTGTCTTCCCACAGTTTGCGAGTGAAGACCGCGGTGTCGTTTTCAATCACATCGGTAAGGGAGATCAGTTTGGTGGCGGGGCAGG              >  SRR4302541.6491145/1‑94 (MQ=50)
                                                                                ACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTG             <  SRR4302541.4776927/94‑1 (MQ=60)
                                                                                ACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTG             <  SRR4302541.8501971/94‑1 (MQ=60)
                                                                                ACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTG             >  SRR4302541.3364503/1‑94 (MQ=60)
                                                                                  TCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCA           >  SRR4302541.3701459/1‑94 (MQ=60)
                                                                                    GGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATG         >  SRR4302541.4632956/1‑94 (MQ=60)
                                                                                    GGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATG         >  SRR4302541.1656498/1‑94 (MQ=60)
                                                                                    GGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATG         <  SRR4302541.7117019/94‑1 (MQ=60)
                                                                                      TGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGAT       >  SRR4302541.5111085/1‑94 (MQ=60)
                                                                                      TGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGAT       <  SRR4302541.8624714/94‑1 (MQ=60)
                                                                                      TGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGAT       >  SRR4302541.2102307/1‑94 (MQ=60)
                                                                                      TGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGAATGAT       >  SRR4302541.6712570/1‑94 (MQ=60)
                                                                                       GCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGATC      <  SRR4302541.7404476/94‑1 (MQ=60)
                                                                                       GCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGATC      >  SRR4302541.2900189/1‑94 (MQ=60)
                                                                                        CTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGATCA     >  SRR4302541.1076269/1‑94 (MQ=60)
                                                                                        CTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGATCA     <  SRR4302541.1051081/94‑1 (MQ=60)
                                                                                        CTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGATCA     >  SRR4302541.6929944/1‑94 (MQ=60)
                                                                                        CTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGATCA     <  SRR4302541.5785375/94‑1 (MQ=60)
                                                                                         TGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGATCAC    >  SRR4302541.5613191/1‑94 (MQ=60)
                                                                                         TGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGATCAC    >  SRR4302541.1552088/1‑94 (MQ=60)
                                                                                           TCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGATCACCC  <  SRR4302541.1924288/94‑1 (MQ=60)
                                                                                           TCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGATCACCC  >  SRR4302541.4235249/1‑94 (MQ=60)
                                                                                           TCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGATCACCC  <  SRR4302541.5499212/94‑1 (MQ=60)
                                                                                           |                                                                                             
TGCGGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGCCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGATCACCC  >  NC_000913/4091260‑4091444

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑