Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 884 257.2 12958910 96.9% 12557183 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,091,351 C→T G168D (GGC→GAC)  rhaD ← rhamnulose‑phosphate aldolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,091,3510CT99.6% 911.7 / NA 237G168D (GGC→GAC) rhaDrhamnulose‑phosphate aldolase
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (118/118);  total (119/118)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 242 total aligned reads displayed.

GCGGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGCCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGATC  >  NC_000913/4091261‑4091440
                                                                                          |                                                                                         
gCGGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCt                                                                                         <  2:2805858/93‑1 (MQ=255)
gCGGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCt                                                                                         >  2:2518995/1‑93 (MQ=255)
  ggTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTc                                                                                       <  2:6234055/93‑1 (MQ=255)
   gTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTcc                                                                                      >  1:3320873/1‑93 (MQ=255)
    tCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTccc                                                                                     <  2:2767893/93‑1 (MQ=255)
     cGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTcccc                                                                                    >  1:2654054/1‑92 (MQ=255)
     cGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCca                                                                                    <  2:2483902/93‑1 (MQ=255)
      gCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCcac                                                                                   <  1:6087595/93‑1 (MQ=255)
       ccTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCcaca                                                                                  >  2:1044755/1‑93 (MQ=255)
        cTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAg                                                                                 >  1:2705876/1‑93 (MQ=255)
         tGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGt                                                                                <  2:2835609/93‑1 (MQ=255)
          ggCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGtt                                                                               <  1:4364976/93‑1 (MQ=255)
          ggCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGtt                                                                               <  1:1873775/93‑1 (MQ=255)
          ggCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGtt                                                                               >  1:2127933/1‑93 (MQ=255)
          ggCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGtt                                                                               >  2:212978/1‑93 (MQ=255)
          ggCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGtt                                                                               >  1:3788133/1‑93 (MQ=255)
          ggCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGtt                                                                               <  2:5038849/93‑1 (MQ=255)
           gCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTg                                                                              <  1:563883/93‑1 (MQ=255)
           gCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTg                                                                              >  1:116069/1‑93 (MQ=255)
            ccGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTgg                                                                             <  1:1366872/93‑1 (MQ=255)
              gATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCg                                                                           >  2:4906741/1‑93 (MQ=255)
              gATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCg                                                                           >  2:371553/1‑93 (MQ=255)
              gATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCg                                                                           >  1:2312605/1‑93 (MQ=255)
                tttCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAg                                                                         <  2:5704674/93‑1 (MQ=255)
                tttCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAg                                                                         <  1:5846687/93‑1 (MQ=255)
                 ttCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGt                                                                        <  1:3513102/93‑1 (MQ=255)
                 ttCGTCCGGGCCGGGCGCCATCCACGGCAAAATGCCAACGCCAGCCGGGAATACCCCCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGt                                                                        <  1:165547/93‑1 (MQ=255)
                  tCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTg                                                                       <  2:4705/93‑1 (MQ=255)
                  tCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTg                                                                       <  1:4796278/93‑1 (MQ=255)
                    gTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGaa                                                                     <  1:4573997/93‑1 (MQ=255)
                     tCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAg                                                                    >  2:4222890/1‑93 (MQ=255)
                      ccGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGa                                                                   <  2:5992746/93‑1 (MQ=255)
                       cGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGAc                                                                  <  1:3437030/93‑1 (MQ=255)
                       cGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGAc                                                                  <  2:3931976/93‑1 (MQ=255)
                        gTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGAcc                                                                 <  2:5117302/93‑1 (MQ=255)
                        gTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGAcc                                                                 <  1:1083783/93‑1 (MQ=255)
                             gggCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGgt                                                            <  2:1941197/93‑1 (MQ=255)
                             aggCACCATCCACGGCAAAAAGCCAACACCATCCGGGCATACAACCAACCCAACGGTGCTGTCTTCCCACAGATGGCGAGTGAAGACCGCGgt                                                            <  2:6472058/92‑1 (MQ=255)
                              ggCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGgtg                                                           >  1:69402/1‑93 (MQ=255)
                               gCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCTAGTGAAGACCGCGgtgt                                                          <  2:831983/93‑1 (MQ=255)
                               gCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGgtgt                                                          <  1:4863303/93‑1 (MQ=255)
                               gCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGgtgt                                                          <  1:2814632/93‑1 (MQ=255)
                               gCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCCGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGgtgt                                                          <  1:5243884/93‑1 (MQ=255)
                                cACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTc                                                         <  2:2302353/93‑1 (MQ=255)
                                cACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTc                                                         <  2:1420770/93‑1 (MQ=255)
                                    aTCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGttt                                                     <  2:4530528/93‑1 (MQ=255)
                                     tCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGtttt                                                    >  2:4783947/1‑93 (MQ=255)
                                     tCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGtttt                                                    <  1:4322172/93‑1 (MQ=255)
                                       cACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCa                                                  >  2:616902/1‑93 (MQ=255)
                                        aCGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCaa                                                 >  1:3571568/1‑93 (MQ=255)
                                          ggCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGt                                               >  1:780076/1‑93 (MQ=255)
                                            cAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTAc                                             <  2:4101867/93‑1 (MQ=255)
                                             aaaaTGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACa                                            >  1:2963338/1‑93 (MQ=255)
                                             aaaaTGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACa                                            <  2:2389986/93‑1 (MQ=255)
                                                aTGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAg                                         >  2:2928935/1‑93 (MQ=255)
                                                aTGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAg                                         <  2:3243297/93‑1 (MQ=255)
                                                  gCCACCGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGt                                       <  1:2534808/93‑1 (MQ=255)
                                                  gCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGt                                       <  1:2419879/93‑1 (MQ=255)
                                                   ccAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTg                                      <  1:330559/93‑1 (MQ=255)
                                                     aaCGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAg                                    >  2:5218777/1‑93 (MQ=255)
                                                      aCGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAgg                                   >  2:2434516/1‑93 (MQ=255)
                                                        gCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGc                                 <  2:5815273/93‑1 (MQ=255)
                                                         ccATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCg                                >  1:3224414/1‑93 (MQ=255)
                                                           aTCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGAt                              <  2:3855164/93‑1 (MQ=255)
                                                           aTCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGAt                              >  1:1009489/1‑93 (MQ=255)
                                                           aTCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGAt                              >  1:1262645/1‑93 (MQ=255)
                                                           aTCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGAt                              >  1:1679542/1‑93 (MQ=255)
                                                           aTCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGAt                              <  1:4953051/93‑1 (MQ=255)
                                                            tCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATc                             >  1:526601/1‑93 (MQ=255)
                                                            tCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATc                             <  1:3603598/93‑1 (MQ=255)
                                                            gccGGGAACGCCCCCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATc                             <  2:1193298/92‑1 (MQ=255)
                                                             ccGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCa                            <  2:3740730/93‑1 (MQ=255)
                                                             ccGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCa                            <  2:3802898/93‑1 (MQ=255)
                                                               gggAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAgg                          >  2:369028/1‑93 (MQ=255)
                                                               gggAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAgg                          >  2:1144999/1‑93 (MQ=255)
                                                               gggAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGACGGCGATCAgg                          >  2:5572547/1‑93 (MQ=255)
                                                                ggAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGt                         <  1:4178511/93‑1 (MQ=255)
                                                                ggAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGt                         >  2:3375059/1‑93 (MQ=255)
                                                                ggAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGt                         <  2:1736605/93‑1 (MQ=255)
                                                                  aaTACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTtg                       <  2:3103429/93‑1 (MQ=255)
                                                                   aTACCACCAGACACTCGGTGCTGTCTTCCCACAGTTTGCGAGTGAAGACCGCGGTGTCGCTTTCAAGTACATAGGTGAGGGCGATCAGGTtgg                      >  1:4024229/1‑93 (MQ=255)
                                                                   aTACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTtgg                      <  2:1836069/93‑1 (MQ=255)
                                                                   aTACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTtgg                      <  2:2258719/93‑1 (MQ=255)
                                                                   aTACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTtgg                      <  2:4359045/93‑1 (MQ=255)
                                                                    tACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTtggt                     <  1:3844252/93‑1 (MQ=255)
                                                                    tACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTtggg                     >  2:3418352/1‑92 (MQ=255)
                                                                      ccaccaGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTtggtgg                   >  1:6338032/1‑93 (MQ=255)
                                                                       caccaGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGgtggc                  <  1:92115/93‑1 (MQ=255)
                                                                        accaGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGgtggcg                 >  2:4069858/1‑93 (MQ=255)
                                                                        accaGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGgtggcg                 >  2:343950/1‑93 (MQ=255)
                                                                        accaGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGgtggcg                 >  2:130833/1‑93 (MQ=255)
                                                                        accaGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGgtggcg                 <  2:747534/93‑1 (MQ=255)
                                                                         ccaGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGgtggcgg                >  1:4732736/1‑92 (MQ=255)
                                                                         ccaGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGgtggagt                >  1:3010411/1‑93 (MQ=255)
                                                                           aGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGgtggcgtgg              >  1:5873159/1‑93 (MQ=255)
                                                                            gACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGgtggcgtggc             <  2:3182304/93‑1 (MQ=255)
                                                                               acTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGt          >  2:3610168/1‑93 (MQ=255)
                                                                                   ggTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCAt      >  1:5466149/1‑93 (MQ=255)
                                                                                   ggTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGCGAGGGCGATCAGGTTGGCGGCGTGGCAGTGCag      >  2:5296891/1‑92 (MQ=255)
                                                                                    gTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATg     >  1:3638490/1‑93 (MQ=255)
                                                                                          |                                                                                         
GCGGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGCCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGATC  >  NC_000913/4091261‑4091440

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGCGGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGCCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGATCACCC  >  NC_000913/4091260‑4091444
                                                                                           |                                                                                             
TGCGCTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCT                                                                                             <  SRR4302534.4053231/94‑1 (MQ=60)
   GGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCC                                                                                          >  SRR4302534.3409674/1‑94 (MQ=60)
   GGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCC                                                                                          >  SRR4302534.5583417/1‑94 (MQ=60)
   GGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCC                                                                                          >  SRR4302534.4742222/1‑94 (MQ=60)
    GTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCC                                                                                         <  SRR4302534.763835/94‑1 (MQ=60)
     TCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCC                                                                                        >  SRR4302534.2724243/1‑94 (MQ=60)
     TCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCA                                                                                        <  SRR4302534.2055927/94‑1 (MQ=60)
       GCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACA                                                                                      <  SRR4302534.601618/94‑1 (MQ=60)
       GCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACA                                                                                      <  SRR4302534.6253133/94‑1 (MQ=60)
        CCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAG                                                                                     >  SRR4302534.4558761/1‑94 (MQ=60)
        CCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAG                                                                                     >  SRR4302534.503515/1‑94 (MQ=60)
        CCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAG                                                                                     >  SRR4302534.2777331/1‑94 (MQ=60)
          TGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTT                                                                                   <  SRR4302534.1714727/94‑1 (MQ=60)
          TGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTT                                                                                   >  SRR4302534.5707760/1‑94 (MQ=60)
          TGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTT                                                                                   >  SRR4302534.5955995/1‑94 (MQ=60)
          TGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTT                                                                                   >  SRR4302534.3889832/1‑94 (MQ=60)
          TGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTT                                                                                   >  SRR4302534.2183983/1‑94 (MQ=60)
           GGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTG                                                                                  >  SRR4302534.976105/1‑94 (MQ=60)
           GGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTG                                                                                  >  SRR4302534.119157/1‑94 (MQ=60)
           GGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTG                                                                                  <  SRR4302534.1205012/94‑1 (MQ=60)
           GGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTG                                                                                  <  SRR4302534.4482664/94‑1 (MQ=60)
           GGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTG                                                                                  <  SRR4302534.1922537/94‑1 (MQ=60)
            GCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGG                                                                                 <  SRR4302534.577750/94‑1 (MQ=60)
             CCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGC                                                                                >  SRR4302534.3043028/1‑94 (MQ=60)
             CCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGC                                                                                <  SRR4302534.1402873/94‑1 (MQ=60)
              CGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCG                                                                               >  SRR4302534.2373974/1‑94 (MQ=60)
              CGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCG                                                                               >  SRR4302534.1731513/1‑94 (MQ=60)
              CGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCG                                                                               >  SRR4302534.3706762/1‑94 (MQ=60)
                 TTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGT                                                                            <  SRR4302534.6005423/94‑1 (MQ=60)
                  TTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTG                                                                           <  SRR4302534.3607611/94‑1 (MQ=60)
                  TTCGTCCGGGCCGGGCGCCATCCACGGCAAAATGCCAACGCCAGCCGGGAATACCCCCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTG                                                                           <  SRR4302534.169889/94‑1 (MQ=60)
                   TCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGA                                                                          <  SRR4302534.4926507/94‑1 (MQ=60)
                    CGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAA                                                                         >  SRR4302534.3970974/1‑94 (MQ=60)
                    CGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAA                                                                         >  SRR4302534.813698/1‑94 (MQ=60)
                     GTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAG                                                                        <  SRR4302534.4698037/94‑1 (MQ=60)
                      TCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGA                                                                       <  SRR4302534.1215763/94‑1 (MQ=60)
                        CGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCTTCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACC                                                                     <  SRR4302534.2173254/94‑1 (MQ=60)
                        CGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACC                                                                     <  SRR4302534.3529393/94‑1 (MQ=60)
                         GTGCCGGGCGCCAGCCACGGCAAAATGCCAACGCCATCCGGGAATCCCCCCAGACACTCGGTGCGGTCTTCCCACAGTTGGCGAGTGAAGACCG                                                                    <  SRR4302534.73981/94‑1 (MQ=60)
                         GTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCG                                                                    <  SRR4302534.1111966/94‑1 (MQ=60)
                          TGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGC                                                                   <  SRR4302534.3481522/94‑1 (MQ=60)
                             CGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGT                                                                >  SRR4302534.6016806/1‑94 (MQ=60)
                              GGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTG                                                               >  SRR4302534.71301/1‑94 (MQ=60)
                                GCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTC                                                             <  SRR4302534.2888630/94‑1 (MQ=60)
                                GCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTC                                                             <  SRR4302534.354490/94‑1 (MQ=60)
                                GCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTC                                                             <  SRR4302534.4995232/94‑1 (MQ=60)
                                GCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCCGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTC                                                             <  SRR4302534.5385515/94‑1 (MQ=60)
                                 CACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCG                                                            <  SRR4302534.2924804/94‑1 (MQ=60)
                                 CACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCG                                                            <  SRR4302534.2378884/94‑1 (MQ=60)
                                 CACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCG                                                            <  SRR4302534.2469451/94‑1 (MQ=60)
                                 CACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCG                                                            <  SRR4302534.5167576/94‑1 (MQ=60)
                                  CCCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGT                                                           <  SRR4302534.3375059/94‑1 (MQ=60)
                                  CCCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGT                                                           <  SRR4302534.4016084/94‑1 (MQ=60)
                                  CCCATCCACGGCAAAATGCCAACCCCATCCGGCAATACCACCAGACACTCGGTGCTGTCTTCCCCCAGTTGGCGAGTGAAGACCGCGGTGTCGT                                                           <  SRR4302534.1484814/94‑1 (MQ=60)
                                    CATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTT                                                         >  SRR4302534.5255601/1‑94 (MQ=60)
                                    CATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTT                                                         >  SRR4302534.3016742/1‑94 (MQ=60)
                                    CATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTT                                                         >  SRR4302534.6291356/1‑94 (MQ=60)
                                     ATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTT                                                        >  SRR4302534.459455/1‑94 (MQ=60)
                                      TCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTC                                                       <  SRR4302534.4439149/94‑1 (MQ=60)
                                       CCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGGGCTGTCGTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGGTTTCA                                                      >  SRR4302534.6645957/1‑94 (MQ=60)
                                        CACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAA                                                     >  SRR4302534.3667632/1‑94 (MQ=60)
                                          CGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGT                                                   >  SRR4302534.799526/1‑94 (MQ=60)
                                             CAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACA                                                >  SRR4302534.3040951/1‑94 (MQ=60)
                                              AAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACAT                                               >  SRR4302534.6408911/1‑94 (MQ=60)
                                              AAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACAT                                               <  SRR4302534.4472312/94‑1 (MQ=60)
                                              AAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACAT                                               <  SRR4302534.4232327/94‑1 (MQ=60)
                                               AAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATA                                              <  SRR4302534.4407736/94‑1 (MQ=60)
                                                AATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAG                                             >  SRR4302534.362710/1‑94 (MQ=60)
                                                 ATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGG                                            >  SRR4302534.3707319/1‑94 (MQ=60)
                                                   GCCACCGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTG                                          <  SRR4302534.2602088/94‑1 (MQ=60)
                                                   GCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTG                                          <  SRR4302534.2484228/94‑1 (MQ=60)
                                                   GCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTG                                          >  SRR4302534.3759086/1‑94 (MQ=60)
                                                    CCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGA                                         >  SRR4302534.2305850/1‑94 (MQ=60)
                                                    CCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGA                                         >  SRR4302534.392421/1‑94 (MQ=60)
                                                    CCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGA                                         <  SRR4302534.339057/94‑1 (MQ=60)
                                                      AACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGG                                       <  SRR4302534.6171813/94‑1 (MQ=60)
                                                      AACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGG                                       >  SRR4302534.4919138/1‑94 (MQ=60)
                                                        CGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGC                                     <  SRR4302534.3400011/94‑1 (MQ=60)
                                                         GCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCG                                    >  SRR4302534.3310060/1‑94 (MQ=60)
                                                          CCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGA                                   <  SRR4302534.3234030/94‑1 (MQ=60)
                                                           CATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGAT                                  >  SRR4302534.3459631/1‑94 (MQ=60)
                                                           CATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGAT                                  >  SRR4302534.2798276/1‑94 (MQ=60)
                                                           CATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGAT                                  >  SRR4302534.1723241/1‑94 (MQ=60)
                                                           CATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGAT                                  >  SRR4302534.1035423/1‑94 (MQ=60)
                                                           CATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGAT                                  >  SRR4302534.1295912/1‑94 (MQ=60)
                                                            ATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATC                                 >  SRR4302534.539675/1‑94 (MQ=60)
                                                            ATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATC                                 <  SRR4302534.5087201/94‑1 (MQ=60)
                                                             TCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCA                                <  SRR4302534.3700500/94‑1 (MQ=60)
                                                              CCGGGAAGACCACCAGACTCTCGGTGCTGTCTACCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAG                               <  SRR4302534.437532/94‑1 (MQ=60)
                                                                GGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGT                             <  SRR4302534.3669921/94‑1 (MQ=60)
                                                                GGGAATACCACCAGACACTCGGTGCTGCCTTCCCACAGCTGGCGAGTGGAGACCGCGGTGACGTTTTCAAGTACATAGGTGAGGGCGATCAGGT                             >  SRR4302534.151672/1‑94 (MQ=60)
                                                                 GGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTT                            <  SRR4302534.4291408/94‑1 (MQ=60)
                                                                   AATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTTGCGAGTGAAGACCGCGGTGTCGCTTTCAAGTACATAGGTGAGGGCGATCAGGTTGG                          >  SRR4302534.4132132/1‑94 (MQ=60)
                                                                     TACCACCAGACTCTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTG                        <  SRR4302534.4971065/94‑1 (MQ=60)
                                                                     TACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTG                        <  SRR4302534.3947502/94‑1 (MQ=60)
                                                                     TACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGCTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTG                        >  SRR4302534.2606561/1‑94 (MQ=60)
                                                                      ACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGG                       >  SRR4302534.6509237/1‑94 (MQ=60)
                                                                      ACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGG                       >  SRR4302534.4990167/1‑94 (MQ=60)
                                                                       CCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGGGAAGACCGCGGTGTCGTTTTCAAGTACATATGTGAGGGCGATCAGGGTGGTGGC                      >  SRR4302534.2750757/1‑94 (MQ=60)
                                                                        CACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCG                     <  SRR4302534.94577/94‑1 (MQ=60)
                                                                         ACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGT                    >  SRR4302534.5404491/1‑94 (MQ=60)
                                                                         ACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGG                    >  SRR4302534.4861249/1‑94 (MQ=60)
                                                                         ACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGAGT                    >  SRR4302534.3089182/1‑94 (MQ=60)
                                                                          CCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTG                   <  SRR4302534.1071746/94‑1 (MQ=60)
                                                                          CCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTG                   <  SRR4302534.2588676/94‑1 (MQ=60)
                                                                           CAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGG                  >  SRR4302534.6032624/1‑94 (MQ=60)
                                                                            AGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGC                 <  SRR4302534.310804/94‑1 (MQ=60)
                                                                             GACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCA                >  SRR4302534.3626732/1‑94 (MQ=60)
                                                                               CACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGT              <  SRR4302534.1301999/94‑1 (MQ=60)
                                                                               CACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGT              >  SRR4302534.1146427/1‑94 (MQ=60)
                                                                                ACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTG             <  SRR4302534.5718795/94‑1 (MQ=60)
                                                                                  TCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCA           <  SRR4302534.2622607/94‑1 (MQ=60)
                                                                                   CGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCAT          >  SRR4302534.5613489/1‑94 (MQ=60)
                                                                                   CGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGAGATCAGGTTGGTGGCGTGGCAGTGCAT          <  SRR4302534.803810/94‑1 (MQ=60)
                                                                                    GGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATG         >  SRR4302534.3736282/1‑94 (MQ=60)
                                                                                         TGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGATCAC    <  SRR4302534.2076372/94‑1 (MQ=60)
                                                                                         TGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGATCAC    <  SRR4302534.3472105/94‑1 (MQ=60)
                                                                                         TGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGATCAC    >  SRR4302534.4088186/1‑94 (MQ=60)
                                                                                           TCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGATCACCC  >  SRR4302534.4299927/1‑94 (MQ=60)
                                                                                           |                                                                                             
TGCGGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGCCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGATCACCC  >  NC_000913/4091260‑4091444

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑