Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 744 338.8 17650688 97.8% 17262372 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,433,048 A→G Y366H (TAT→CAT)  cpdB ← 2',3'‑cyclic‑nucleotide 2'‑phosphodiesterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,433,0480AG99.2% 1390.1 / ‑3.8 402Y366H (TAT→CAT) cpdB2',3'‑cyclic‑nucleotide 2'‑phosphodiesterase
Reads supporting (aligned to +/- strand):  ref base A (0/3);  new base G (181/218);  total (181/221)
Fisher's exact test for biased strand distribution p-value = 2.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 411 total aligned reads displayed.

CCGGATCGCCCTGAATGTAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATAGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAGTGTTTCTACCAGCT  >  NC_000913/4432958‑4433137
                                                                                          |                                                                                         
 cGGATCGCCCTGAATGTAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCt                                                                                        <  2:1223989/93‑1 (MQ=255)
  ggATCGCCCTGAATGTAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCta                                                                                       <  1:8403844/93‑1 (MQ=255)
  ggATCGCCCTGAATGTAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCta                                                                                       >  2:505010/1‑93 (MQ=255)
  cgATCGCCCTGAATGTAATGCTCTACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCta                                                                                       <  1:4647471/92‑1 (MQ=255)
     tCGCCCTGAATGTAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATAc                                                                                    >  2:2035980/1‑93 (MQ=255)
     tCGCCCTGAATGTAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATAc                                                                                    >  2:1966502/1‑93 (MQ=255)
      cGCCCTGAATGTAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACa                                                                                   <  1:7870343/93‑1 (MQ=255)
       gCCCTGAATGTAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACat                                                                                  <  2:178010/93‑1 (MQ=255)
        cccTGAATGTAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACata                                                                                 >  2:6874440/1‑93 (MQ=255)
          cTGAATGTAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATAtt                                                                               <  2:844388/93‑1 (MQ=255)
          cTGAATGTAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATAtt                                                                               <  2:3795747/93‑1 (MQ=255)
           tGAATGTAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTg                                                                              >  2:4262530/1‑93 (MQ=255)
           tGAATGTAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTg                                                                              <  1:2850093/93‑1 (MQ=255)
             aaTGTAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTc                                                                            <  2:3193528/93‑1 (MQ=255)
             aaTGTAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTc                                                                            <  2:5598088/93‑1 (MQ=255)
              aTGTAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCg                                                                           >  1:8713273/1‑93 (MQ=255)
              aTGTAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCg                                                                           >  1:5279687/1‑93 (MQ=255)
              aTGTAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCg                                                                           >  2:3285765/1‑93 (MQ=255)
              aTGTAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCg                                                                           <  1:4418337/93‑1 (MQ=255)
                gTAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGc                                                                         >  1:105611/1‑93 (MQ=255)
                gTAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGc                                                                         >  1:4147244/1‑93 (MQ=255)
                  aaTGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAg                                                                       >  2:883684/1‑93 (MQ=255)
                   aTGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGa                                                                      <  1:7137701/93‑1 (MQ=255)
                        cGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTAc                                                                 <  1:3649834/93‑1 (MQ=255)
                        cGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTAc                                                                 <  1:3506290/93‑1 (MQ=255)
                        cGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTAc                                                                 <  1:2097379/93‑1 (MQ=255)
                         gACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTAcc                                                                <  1:8488499/93‑1 (MQ=255)
                          aCATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCg                                                               <  2:7686299/93‑1 (MQ=255)
                            aTACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGAt                                                             <  1:88431/93‑1 (MQ=255)
                            aTACGCTTGTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCATCAGCGCAAGGTGGCTATACATATTGTCGAAAGATTTACCGAt                                                             <  2:8068788/93‑1 (MQ=255)
                             tACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGAtt                                                            >  1:4948675/1‑93 (MQ=255)
                             tACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGAtt                                                            >  2:6644633/1‑93 (MQ=255)
                             tACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGAtt                                                            <  1:1642621/93‑1 (MQ=255)
                               cGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTgg                                                          <  1:2353732/93‑1 (MQ=255)
                                gCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGc                                                         <  1:4748738/93‑1 (MQ=255)
                                gCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGc                                                         <  2:7117187/93‑1 (MQ=255)
                                gCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGc                                                         >  1:3951912/1‑93 (MQ=255)
                                 cTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCt                                                        >  1:2428650/1‑93 (MQ=255)
                                  tttttGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCtt                                                       >  2:2370043/1‑93 (MQ=255)
                                     ttGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCt                                                    <  2:83024/93‑1 (MQ=255)
                                       gcgcGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGa                                                  >  1:2778950/1‑93 (MQ=255)
                                       gcgcGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGa                                                  <  2:3030713/93‑1 (MQ=255)
                                        cgcgTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGAc                                                 <  2:3877117/93‑1 (MQ=255)
                                         gcgTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACg                                                <  1:7724917/93‑1 (MQ=255)
                                           gttgttCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGaa                                              <  2:2260226/93‑1 (MQ=255)
                                           gttgttCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGaa                                              <  2:5313908/93‑1 (MQ=255)
                                           gttgttCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGaa                                              >  1:2581241/1‑93 (MQ=255)
                                            ttgttCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAAc                                             <  1:8026815/93‑1 (MQ=255)
                                              gttCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTg                                           >  1:2996705/1‑93 (MQ=255)
                                                tCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGc                                         >  2:1097165/1‑93 (MQ=255)
                                                tCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGc                                         <  2:2177146/93‑1 (MQ=255)
                                                tCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGc                                         <  2:3406261/93‑1 (MQ=255)
                                                  accaccTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCgt                                       <  2:5271653/93‑1 (MQ=255)
                                                  accaccTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCgg                                       >  1:3878785/1‑92 (MQ=255)
                                                   ccaccTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCgtg                                      >  1:695758/1‑93 (MQ=255)
                                                    caccTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCgggg                                     >  2:6591905/1‑90 (MQ=255)
                                                      ccTGCACGGTCGGATCGTCATGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTgg                                   <  1:1967921/93‑1 (MQ=255)
                                                        tGCACGGTCGGATTGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCa                                 <  1:5184516/93‑1 (MQ=255)
                                                        tGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCa                                 >  1:2210597/1‑93 (MQ=255)
                                                        tGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCa                                 >  2:1409148/1‑93 (MQ=255)
                                                        tGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCCTGCTGACGAACTGGCGTGTGGCa                                 >  2:4737556/1‑93 (MQ=255)
                                                         gCACGGTCGGATCGTCCCGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCAt                                <  2:5999990/93‑1 (MQ=255)
                                                          cACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATc                               >  1:2639033/1‑93 (MQ=255)
                                                          cACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATc                               <  1:6869899/93‑1 (MQ=255)
                                                            cGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGt                             >  1:680178/1‑93 (MQ=255)
                                                             ggTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTg                            >  1:652989/1‑93 (MQ=255)
                                                              gTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGa                           <  2:7942765/93‑1 (MQ=255)
                                                              gTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGa                           <  2:6787310/93‑1 (MQ=255)
                                                              gTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGa                           >  1:1175085/1‑93 (MQ=255)
                                                               tCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGAt                          <  2:7551491/93‑1 (MQ=255)
                                                               tCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGAt                          <  1:666374/93‑1 (MQ=255)
                                                                cGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATc                         >  2:4844052/1‑93 (MQ=255)
                                                                   aTCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGAAGTACTGGTGTGTGGCGTCGGTATCgct                      >  1:5928401/1‑91 (MQ=255)
                                                                    tCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCt                     >  2:6338774/1‑93 (MQ=255)
                                                                     cGTCTTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCtt                    <  1:3996457/93‑1 (MQ=255)
                                                                     cGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCtt                    <  1:1608601/93‑1 (MQ=255)
                                                                     cGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCtt                    <  1:8103118/93‑1 (MQ=255)
                                                                       tCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTg                  >  1:3190854/1‑93 (MQ=255)
                                                                       tCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTg                  >  1:6118175/1‑93 (MQ=255)
                                                                       tCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCAATTCACATACTGACGAACAGGTGTAGGGTATGGTGAACGGCTTTg                  >  2:6240371/1‑93 (MQ=255)
                                                                        ccTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGa                 <  1:7263111/93‑1 (MQ=255)
                                                                        ccTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGa                 <  2:5764745/93‑1 (MQ=255)
                                                                         cTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAg                >  1:3321851/1‑93 (MQ=255)
                                                                          tGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAgt               <  1:5612060/93‑1 (MQ=255)
                                                                           gCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAgtg              >  1:5345701/1‑93 (MQ=255)
                                                                           gCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAggg              >  1:2510775/1‑91 (MQ=255)
                                                                            cACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAgtgt             <  2:3531336/93‑1 (MQ=255)
                                                                            cACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAgtgt             >  2:8663504/1‑93 (MQ=255)
                                                                              ccAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAGTGttt           <  1:1353885/93‑1 (MQ=255)
                                                                               cAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAGTGTTTc          >  2:4495725/1‑93 (MQ=255)
                                                                               cAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAGTGTTTc          <  2:2727855/93‑1 (MQ=255)
                                                                                 gcgcCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGGGTGTTTCTa        >  1:7380289/1‑93 (MQ=255)
                                                                                  cgcCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAGTGTTTCTAc       <  1:8232964/93‑1 (MQ=255)
                                                                                  cgcCAGATGGCTATACATATTGCCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAGTGTTTCTAc       <  2:4152556/93‑1 (MQ=255)
                                                                                   gcCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAGTGTTTCTAcc      <  1:3739009/93‑1 (MQ=255)
                                                                                    ccAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAGTGTTTCTACCa     <  1:642594/93‑1 (MQ=255)
                                                                                      aGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAGTGTTTCTACCAGc   >  1:4183819/1‑93 (MQ=255)
                                                                                      aGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAGTGTTTCTACCAGc   <  2:2317122/93‑1 (MQ=255)
                                                                                       gATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAGTGTTTCTACCAGCt  <  1:4276979/93‑1 (MQ=255)
                                                                                       gATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAGTGTTTCTACCAGCt  >  2:1039486/1‑93 (MQ=255)
                                                                                          |                                                                                         
CCGGATCGCCCTGAATGTAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATAGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAGTGTTTCTACCAGCT  >  NC_000913/4432958‑4433137

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 245 total aligned reads displayed.
GATCCGGATCGCCCTGAATGTAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATAGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAGTGTTTCTACCAGCTTGCT  >  NC_000913/4432955‑4433141
                                                                                             |                                                                                             
 ATCCGGATCGCCCTGAATGTAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGG                                                                                              <  SRR4302541.2772787/94‑1 (MQ=60)
  TCCGGATCGCCCTGAATGTAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGC                                                                                             <  SRR4302541.1211764/94‑1 (MQ=60)
  TCCGGATCGCCCTGAATGTAATGCTCGACATACGCTTTTTGCGCGGTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGC                                                                                             >  SRR4302541.4095598/1‑94 (MQ=60)
  TCCGGATCGCCCTGAATGTAATGCGCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGC                                                                                             <  SRR4302541.1211763/94‑1 (MQ=60)
    CGGATCGCCCTGAATGTAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTA                                                                                           >  SRR4302541.6647098/1‑94 (MQ=60)
     GGATCGCCCTGAATGTAATGCTCGACATGCGCTTTTTGCGCGTTGTTCACCACCTGCCCGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTAT                                                                                          <  SRR4302541.2250541/94‑1 (MQ=60)
     GGATCGCCCTGAATGTAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTAT                                                                                          <  SRR4302541.4109455/94‑1 (MQ=60)
     GGATCGCCCTGAATGTAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATAGCTAT                                                                                          <  SRR4302541.3420597/94‑1 (MQ=60)
     CGATCGCCCTGAATGTAATGCTCTACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTAT                                                                                          <  SRR4302541.4694796/94‑1 (MQ=60)
      GATCGCCCTGAATGTAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATA                                                                                         >  SRR4302541.3676510/1‑94 (MQ=60)
      GATCGCCCTGAATGTAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATA                                                                                         <  SRR4302541.2739279/94‑1 (MQ=60)
      GATCCCCCTGAATGTAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATA                                                                                         <  SRR4302541.2739278/94‑1 (MQ=60)
       ATCGCCCTGAATGTAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATAC                                                                                        >  SRR4302541.7995996/1‑94 (MQ=60)
        TCGCCCTGAATGTAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACA                                                                                       <  SRR4302541.2107065/94‑1 (MQ=60)
        TCGCCCTGAATGTAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACA                                                                                       <  SRR4302541.5200225/94‑1 (MQ=60)
         CGCCCTGAATGTAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACAT                                                                                      <  SRR4302541.7956817/94‑1 (MQ=60)
         CGCCCTGAATGTAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACAT                                                                                      <  SRR4302541.3277041/94‑1 (MQ=60)
         CGCCCTGAATGTAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACAT                                                                                      >  SRR4302541.3942571/1‑94 (MQ=60)
          GCCCTGAATGTAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATA                                                                                     <  SRR4302541.8116278/94‑1 (MQ=60)
           CCCTGAATGTAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATAT                                                                                    >  SRR4302541.4950997/1‑94 (MQ=60)
            CCTTAATGTAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATT                                                                                   <  SRR4302541.355344/94‑1 (MQ=60)
            CCTGAATGTAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATT                                                                                   >  SRR4302541.2705999/1‑94 (MQ=60)
            CCTGAATGTAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATT                                                                                   <  SRR4302541.2830962/94‑1 (MQ=60)
             CTGAATGTAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTG                                                                                  >  SRR4302541.4412906/1‑94 (MQ=60)
              TGAATGTAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGT                                                                                 <  SRR4302541.2878757/94‑1 (MQ=60)
              TGAATGTAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGT                                                                                 <  SRR4302541.4210399/94‑1 (MQ=60)
               GAATGTAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTC                                                                                >  SRR4302541.8551719/1‑94 (MQ=60)
                AATGTAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCG                                                                               >  SRR4302541.5334049/1‑94 (MQ=60)
                AATGTAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCG                                                                               >  SRR4302541.8806484/1‑94 (MQ=60)
                 ATGTAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGG                                                                              <  SRR4302541.4462667/94‑1 (MQ=60)
                 ATGTAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGG                                                                              >  SRR4302541.7361531/1‑94 (MQ=60)
                  TGTAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGC                                                                             >  SRR4302541.3769544/1‑94 (MQ=60)
                  TGTAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGC                                                                             >  SRR4302541.4188237/1‑94 (MQ=60)
                  TGTAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGC                                                                             >  SRR4302541.106491/1‑94 (MQ=60)
                   GTAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCA                                                                            <  SRR4302541.3024120/94‑1 (MQ=60)
                   GTAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCA                                                                            >  SRR4302541.6869296/1‑94 (MQ=60)
                    TAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAG                                                                           >  SRR4302541.5750793/1‑94 (MQ=60)
                      ATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGAT                                                                         <  SRR4302541.4349268/94‑1 (MQ=60)
                      ATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGAT                                                                         <  SRR4302541.7213761/94‑1 (MQ=60)
                       TGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATT                                                                        <  SRR4302541.1578583/94‑1 (MQ=60)
                        TCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTT                                                                       <  SRR4302541.261901/94‑1 (MQ=60)
                         CTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTA                                                                      >  SRR4302541.2036959/1‑94 (MQ=60)
                          TCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTAC                                                                     <  SRR4302541.373955/94‑1 (MQ=60)
                          TCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTAC                                                                     <  SRR4302541.4216932/94‑1 (MQ=60)
                          TCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTAC                                                                     <  SRR4302541.6913692/94‑1 (MQ=60)
                          TCGACAGACGCTTTTTGCGCGTTGTTCACCGCCTGCACGGTCGGATCCTCCTGCACCAGCGCCAGATGGCTATGCATATTGTCGGCAGATTTAC                                                                     <  SRR4302541.4216929/94‑1 (MQ=60)
                          CCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCCCCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTAC                                                                     <  SRR4302541.4835684/94‑1 (MQ=60)
                           cgctattcgcttttttgGCGTTGTTCGCCACCTGCACGGTCGGATCTTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACC                                                                    <  SRR4302541.1981188/77‑1 (MQ=60)
                           CGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACC                                                                    <  SRR4302541.3541504/94‑1 (MQ=60)
                           CGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACC                                                                    <  SRR4302541.2117708/94‑1 (MQ=60)
                           CGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACC                                                                    <  SRR4302541.3686861/94‑1 (MQ=60)
                            GACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCG                                                                   <  SRR4302541.7052700/94‑1 (MQ=60)
                            GACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCG                                                                   <  SRR4302541.8579974/94‑1 (MQ=60)
                            GACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCG                                                                   >  SRR4302541.4007566/1‑94 (MQ=60)
                             ACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGA                                                                  >  SRR4302541.2608576/1‑94 (MQ=60)
                             ACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGA                                                                  >  SRR4302541.6979241/1‑94 (MQ=60)
                              CATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACGGAT                                                                 >  SRR4302541.2996678/1‑94 (MQ=60)
                              CATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGAT                                                                 >  SRR4302541.2996679/1‑94 (MQ=60)
                              CATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGAT                                                                 <  SRR4302541.8269529/94‑1 (MQ=60)
                               ATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACCTATTGTCGGCAGATTTACCGATT                                                                >  SRR4302541.6485176/1‑94 (MQ=60)
                               ATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATT                                                                >  SRR4302541.4999248/1‑94 (MQ=60)
                               ATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATT                                                                <  SRR4302541.545117/94‑1 (MQ=60)
                               ATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATT                                                                >  SRR4302541.3740715/1‑94 (MQ=60)
                               ATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATT                                                                <  SRR4302541.89205/94‑1 (MQ=60)
                                TACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTG                                                               <  SRR4302541.7631047/94‑1 (MQ=60)
                                TACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTG                                                               <  SRR4302541.1658866/94‑1 (MQ=60)
                                 ACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGG                                                              <  SRR4302541.8423405/94‑1 (MQ=60)
                                 ACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGG                                                              <  SRR4302541.390546/94‑1 (MQ=60)
                                 ACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGG                                                              >  SRR4302541.6609315/1‑94 (MQ=60)
                                  CGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGC                                                             >  SRR4302541.3991204/1‑94 (MQ=60)
                                  CGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGC                                                             <  SRR4302541.2376645/94‑1 (MQ=60)
                                   GCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCT                                                            <  SRR4302541.8535993/94‑1 (MQ=60)
                                   GCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCT                                                            <  SRR4302541.4797253/94‑1 (MQ=60)
                                   GCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCT                                                            >  SRR4302541.2452663/1‑94 (MQ=60)
                                    CTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTT                                                           >  SRR4302541.5403307/1‑94 (MQ=60)
                                    CTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTT                                                           >  SRR4302541.7893193/1‑94 (MQ=60)
                                    CTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTT                                                           <  SRR4302541.264781/94‑1 (MQ=60)
                                     TTTTTGCCCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTG                                                          <  SRR4302541.3777657/94‑1 (MQ=60)
                                       TTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCT                                                        <  SRR4302541.7593764/94‑1 (MQ=60)
                                         TGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGA                                                      <  SRR4302541.2425404/94‑1 (MQ=60)
                                          GCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGAC                                                     <  SRR4302541.1764985/94‑1 (MQ=60)
                                          GCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGAC                                                     <  SRR4302541.4306321/94‑1 (MQ=60)
                                          GCGCGTTGTTCACCACATGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGAC                                                     <  SRR4302541.7209938/94‑1 (MQ=60)
                                            GCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGA                                                   >  SRR4302541.4567584/1‑94 (MQ=60)
                                            GCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGA                                                   >  SRR4302541.2178841/1‑94 (MQ=60)
                                            GCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGA                                                   <  SRR4302541.1403933/94‑1 (MQ=60)
                                            GCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGA                                                   <  SRR4302541.7807105/94‑1 (MQ=60)
                                             CGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAA                                                  <  SRR4302541.6064768/94‑1 (MQ=60)
                                             CGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAA                                                  >  SRR4302541.2607113/1‑94 (MQ=60)
                                              GTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAAC                                                 >  SRR4302541.7982030/1‑94 (MQ=60)
                                               TTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGTTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACT                                                >  SRR4302541.4487130/1‑94 (MQ=60)
                                               TTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACT                                                <  SRR4302541.8765407/94‑1 (MQ=60)
                                               TTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACT                                                <  SRR4302541.7677670/94‑1 (MQ=60)
                                               TTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACT                                                <  SRR4302541.658082/94‑1 (MQ=60)
                                               TTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACT                                                <  SRR4302541.8115062/94‑1 (MQ=60)
                                                TGTTCACCAGCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTG                                               <  SRR4302541.4635372/94‑1 (MQ=60)
                                                TGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTCCCGATTGGCTTGCTGACGAACTG                                               <  SRR4302541.6478287/94‑1 (MQ=60)
                                                TGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTG                                               >  SRR4302541.3026953/1‑94 (MQ=60)
                                                TGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTG                                               <  SRR4302541.7356902/94‑1 (MQ=60)
                                                TGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTG                                               >  SRR4302541.5272679/1‑94 (MQ=60)
                                                 GTTCGCCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGG                                              <  SRR4302541.893235/94‑1 (MQ=60)
                                                 GTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGG                                              >  SRR4302541.969743/1‑94 (MQ=60)
                                                  TTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTagcaaactggg                                             >  SRR4302541.3966677/1‑83 (MQ=60)
                                                   TCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCG                                            >  SRR4302541.2858608/1‑94 (MQ=60)
                                                    CACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGG                                           >  SRR4302541.3917742/1‑94 (MQ=60)
                                                    CACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTAGCTTGCTGACGttctggagt                                           >  SRR4302541.4279068/1‑85 (MQ=60)
                                                     ACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGTCTATACCTATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACGGGCGTG                                          >  SRR4302541.2813776/1‑94 (MQ=60)
                                                     ACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTG                                          >  SRR4302541.702303/1‑94 (MQ=60)
                                                     ACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGGG                                          >  SRR4302541.2813775/1‑94 (MQ=60)
                                                      CCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCcggg                                         >  SRR4302541.233820/1‑90 (MQ=60)
                                                      CCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGT                                         >  SRR4302541.6080865/1‑94 (MQ=60)
                                                      CCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGG                                         >  SRR4302541.5197210/1‑94 (MQ=60)
                                                       CACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTTGCTTGCTGACGAACTGGCGTGTG                                        >  SRR4302541.8510987/1‑94 (MQ=60)
                                                       CACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTG                                        >  SRR4302541.2552583/1‑94 (MQ=60)
                                                       CACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTG                                        >  SRR4302541.7011070/1‑94 (MQ=60)
                                                       CACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTG                                        >  SRR4302541.5317203/1‑94 (MQ=60)
                                                       CACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTG                                        >  SRR4302541.5859205/1‑94 (MQ=60)
                                                        CCCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGG                                       <  SRR4302541.1076020/94‑1 (MQ=60)
                                                         CCTGCACGGTCGGATCGTCATGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGC                                      <  SRR4302541.1986900/94‑1 (MQ=60)
                                                          CTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCA                                     >  SRR4302541.2232028/1‑94 (MQ=60)
                                                          CTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCA                                     <  SRR4302541.2338511/94‑1 (MQ=60)
                                                          CTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCA                                     <  SRR4302541.6004324/94‑1 (MQ=60)
                                                           TGCACGGTCGGATTGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCAT                                    <  SRR4302541.5237853/94‑1 (MQ=60)
                                                           TGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCAT                                    >  SRR4302541.358699/1‑94 (MQ=60)
                                                           TGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCAT                                    <  SRR4302541.4888406/94‑1 (MQ=60)
                                                             CACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCG                                  <  SRR4302541.6943285/94‑1 (MQ=60)
                                                              ACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGT                                 >  SRR4302541.686595/1‑94 (MQ=60)
                                                              ACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGT                                 >  SRR4302541.378393/1‑94 (MQ=60)
                                                               CGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTG                                >  SRR4302541.659154/1‑94 (MQ=60)
                                                                GGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGA                               >  SRR4302541.4313992/1‑94 (MQ=60)
                                                                GGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGA                               >  SRR4302541.1186433/1‑94 (MQ=60)
                                                                 GTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGAT                              >  SRR4302541.2007663/1‑94 (MQ=60)
                                                                  TCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATC                             <  SRR4302541.8454410/94‑1 (MQ=60)
                                                                  TCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATC                             <  SRR4302541.672654/94‑1 (MQ=60)
                                                                   CGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCG                            <  SRR4302541.647850/94‑1 (MQ=60)
                                                                    GGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGG                           >  SRR4302541.2598435/1‑94 (MQ=60)
                                                                       TCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTT                        >  SRR4302541.7234576/1‑94 (MQ=60)
                                                                       TCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTT                        >  SRR4302541.1176578/1‑94 (MQ=60)
                                                                        CGTCTTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTT                       <  SRR4302541.4036024/94‑1 (MQ=60)
                                                                        CGTCCTGCCCCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTT                       <  SRR4302541.8382513/94‑1 (MQ=60)
                                                                        CGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCGTCGTGATCGGCTTT                       >  SRR4302541.1637694/1‑94 (MQ=60)
                                                                        CGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTT                       <  SRR4302541.6835068/94‑1 (MQ=60)
                                                                        CGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTT                       <  SRR4302541.2409587/94‑1 (MQ=60)
                                                                        CGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTT                       <  SRR4302541.1624590/94‑1 (MQ=60)
                                                                        CGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTT                       <  SRR4302541.8191935/94‑1 (MQ=60)
                                                                         GTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTG                      >  SRR4302541.3223090/1‑94 (MQ=60)
                                                                         GTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTG                      <  SRR4302541.938967/94‑1 (MQ=60)
                                                                         GTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTG                      <  SRR4302541.5380263/94‑1 (MQ=60)
                                                                         GTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTG                      >  SRR4302541.1572951/1‑94 (MQ=60)
                                                                         GTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTG                      >  SRR4302541.3223091/1‑94 (MQ=60)
                                                                         GTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTG                      <  SRR4302541.5867424/94‑1 (MQ=60)
                                                                         GTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTG                      >  SRR4302541.6180659/1‑94 (MQ=60)
                                                                          TCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGG                     >  SRR4302541.827839/1‑94 (MQ=60)
                                                                          TCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGA                     <  SRR4302541.1656938/94‑1 (MQ=60)
                                                                           CCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAG                    >  SRR4302541.3355163/1‑94 (MQ=60)
                                                                           CCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAG                    <  SRR4302541.8864827/94‑1 (MQ=60)
                                                                           CCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAG                    <  SRR4302541.6232122/94‑1 (MQ=60)
                                                                           CCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAG                    <  SRR4302541.7340591/94‑1 (MQ=60)
                                                                            CTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAGT                   <  SRR4302541.6881729/94‑1 (MQ=60)
                                                                            CTGCACCAGCGCCAGATGGCTATACATATTGGCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAGT                   <  SRR4302541.6881727/94‑1 (MQ=60)
                                                                             TGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAGTG                  <  SRR4302541.5669510/94‑1 (MQ=60)
                                                                             TGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAGTG                  <  SRR4302541.6572753/94‑1 (MQ=60)
                                                                             TGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAGTG                  >  SRR4302541.5400655/1‑94 (MQ=60)
                                                                             TGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAGGG                  >  SRR4302541.2536073/1‑94 (MQ=60)
                                                                              GCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGGGGTCGGCTTTGcgcgt                 >  SRR4302541.71434/1‑89 (MQ=60)
                                                                               CACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAGTGTT                >  SRR4302541.81223/1‑94 (MQ=60)
                                                                               CACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAGTGTT                >  SRR4302541.1152493/1‑94 (MQ=60)
                                                                               CACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAGTGTT                <  SRR4302541.2203140/94‑1 (MQ=60)
                                                                               CACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAGTGGT                >  SRR4302541.3411876/1‑94 (MQ=60)
                                                                                ACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGNANCGTGATCGGCTTTNAGGGNTN               >  SRR4302541.7386873/1‑94 (MQ=60)
                                                                                ACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAGTGTTT               <  SRR4302541.328310/94‑1 (MQ=60)
                                                                                ACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAGTGTTT               <  SRR4302541.344993/94‑1 (MQ=60)
                                                                                ACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGCGTGGCATCGTGATCGGCGTTGAGTGTTT               >  SRR4302541.8184622/1‑94 (MQ=60)
                                                                                 CCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAGTGTTTC              <  SRR4302541.1852345/94‑1 (MQ=60)
                                                                                   AGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGGGTGTTTCTA            >  SRR4302541.7459184/1‑94 (MQ=60)
                                                                                   AGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAGTGTTTCTA            <  SRR4302541.2485259/94‑1 (MQ=60)
                                                                                   AGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAGTGTTTCTA            <  SRR4302541.1307361/94‑1 (MQ=60)
                                                                                   AGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAGTGTTTCTA            <  SRR4302541.4028955/94‑1 (MQ=60)
                                                                                     CGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAGTGTTTCTACC          <  SRR4302541.4733518/94‑1 (MQ=60)
                                                                                     CGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAGTGTTTCTACC          <  SRR4302541.8322708/94‑1 (MQ=60)
                                                                                      GCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAGTGTTTCTACCA         <  SRR4302541.2467555/94‑1 (MQ=60)
                                                                                      GCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAGTGTTTCTACCA         <  SRR4302541.7215350/94‑1 (MQ=60)
                                                                                      GCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAGTGTTTCTACCA         >  SRR4302541.8857180/1‑94 (MQ=60)
                                                                                       CCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAGTGTTTCTACCAG        <  SRR4302541.1239007/94‑1 (MQ=60)
                                                                                       CCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAGTGTTTCTACCAG        <  SRR4302541.5371312/94‑1 (MQ=60)
                                                                                       CCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAGTGTTTCTACCAG        <  SRR4302541.648660/94‑1 (MQ=60)
                                                                                        CAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAGTGTTTCTACCAGC       <  SRR4302541.2524871/94‑1 (MQ=60)
                                                                                        CAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAGTGTTTCTACCAGC       >  SRR4302541.308859/1‑94 (MQ=60)
                                                                                        CAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTGTGAGTGTTTCTACCAGC       >  SRR4302541.7914936/1‑94 (MQ=60)
                                                                                        CAGATGGCTATACATATTGCCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAGTGTTTCTACCAGC       <  SRR4302541.8661161/94‑1 (MQ=60)
                                                                                         AGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAGTGTTTCTACCAGCT      <  SRR4302541.4715896/94‑1 (MQ=60)
                                                                                          GATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAGTGTTTCTACCAGCTT     >  SRR4302541.543213/1‑94 (MQ=60)
                                                                                          GATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAGTGTTTCTACCAGCTT     <  SRR4302541.4319705/94‑1 (MQ=60)
                                                                                           ATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAGTGTTTCTACCAGCTTG    >  SRR4302541.8088245/1‑94 (MQ=60)
                                                                                           ATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAGTGTTTCTACCAGCTTG    >  SRR4302541.1495264/1‑94 (MQ=60)
                                                                                           ATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAGTGTTTCTACCAGCTTG    <  SRR4302541.393012/94‑1 (MQ=60)
                                                                                           ATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAGTGTTTCTACCAGCTTG    <  SRR4302541.3430135/94‑1 (MQ=60)
                                                                                           ATAGATATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGAGGAACTGGCGGGGGGGAGTGTGATCGGCTTTGAGGGTTTCTACgctcttg    >  SRR4302541.8136132/1‑87 (MQ=52)
                                                                                             GGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAGTGTTTCTACCAGCTTGCT  >  SRR4302541.4208117/1‑94 (MQ=60)
                                                                                             GGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAGTGTTTCTACCAGCTTGCT  >  SRR4302541.5367483/1‑94 (MQ=60)
                                                                                             |                                                                                             
GATCCGGATCGCCCTGAATGTAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATAGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAGTGTTTCTACCAGCTTGCT  >  NC_000913/4432955‑4433141

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑