Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	884	257.2	12958910	96.9%	12557183	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,433,048	A→G	Y366H (TAT→CAT)	cpdB ←	2',3'‑cyclic‑nucleotide 2'‑phosphodiesterase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,433,048	0	A	G	100.0%	887.1 / NA	249	Y366H (TAT→CAT)	cpdB	2',3'‑cyclic‑nucleotide 2'‑phosphodiesterase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (125/124); total (125/124)

Only 100 of 255 total aligned reads displayed.

CCGGATCGCCCTGAATGTAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATAGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAGTGTTTCTACCAGCT  >  NC_000913/4432958‑4433137

 cGGATCGCCCTGAATGTAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCt                                                                                        <  1:5425584/93‑1 (MQ=255)

   gATCGCCCTGAATGTAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCtat                                                                                      >  1:6185437/1‑93 (MQ=255)

   gATCGCCCTGAATGTAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCtat                                                                                      >  1:5869734/1‑93 (MQ=255)

   gATCGCCCTGAATGTAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCtat                                                                                      >  2:2570971/1‑93 (MQ=255)

    aTCGCCCTGAATGTAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCtata                                                                                     >  2:1478932/1‑93 (MQ=255)

      gtcccTGAATATAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACa                                                                                   <  1:6029200/91‑1 (MQ=255)

       gCCCTGAATGTAGTGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACat                                                                                  <  2:1622150/93‑1 (MQ=255)

        cccTGAATGTAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACata                                                                                 <  2:1702482/93‑1 (MQ=255)

         ccTGAATGTAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACatat                                                                                >  1:5162664/1‑93 (MQ=255)

         ccTGAATGTAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACatat                                                                                >  2:519281/1‑93 (MQ=255)

          ctaaaTGTAATGCTCGACATGCGCTTTTTGCGCGTTGTTCACCACCTGCTCGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATAtt                                                                               <  1:3444310/90‑1 (MQ=255)

          cTGAATGTAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATAtt                                                                               >  2:2753564/1‑93 (MQ=255)

            gAATGTAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGt                                                                             >  2:6293000/1‑93 (MQ=255)

             aaTGTAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTc                                                                            >  1:2537190/1‑93 (MQ=255)

             aaTGTAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTc                                                                            <  1:4738513/93‑1 (MQ=255)

              aTGTAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCTTCCTGCACCAGCGCCAGATGGCTATACATATTGTCg                                                                           >  2:1078801/1‑93 (MQ=255)

               tGTAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCgg                                                                          <  2:2705059/93‑1 (MQ=255)

                  aaTGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAg                                                                       <  1:281554/93‑1 (MQ=255)

                   aTGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGa                                                                      >  1:3059897/1‑93 (MQ=255)

                       tCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGGTTTa                                                                  >  1:2636749/1‑93 (MQ=255)

                       tCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTa                                                                  >  1:460187/1‑93 (MQ=255)

                       tCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTa                                                                  >  1:2888674/1‑93 (MQ=255)

                        cGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTAc                                                                 <  1:494547/93‑1 (MQ=255)

                        cGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTAc                                                                 >  1:597046/1‑93 (MQ=255)

                        cGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTAc                                                                 <  1:1989993/93‑1 (MQ=255)

                         gACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTAcc                                                                <  1:3685398/93‑1 (MQ=255)

                         gACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTAcc                                                                <  1:5619300/93‑1 (MQ=255)

                           cATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGa                                                              >  1:3508894/1‑93 (MQ=255)

                           cATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGa                                                              >  1:393199/1‑93 (MQ=255)

                           cATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGa                                                              >  1:416886/1‑93 (MQ=255)

                           cATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGCCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGa                                                              <  2:4691875/93‑1 (MQ=255)

                            aTACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTACACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGAt                                                             >  1:1065964/1‑93 (MQ=255)

                             tACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGAtt                                                            <  1:39599/93‑1 (MQ=255)

                               cGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTgg                                                          >  2:137907/1‑93 (MQ=255)

                                gCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGc                                                         >  2:1465457/1‑93 (MQ=255)

                                 cTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCt                                                        >  2:341541/1‑93 (MQ=255)

                                 cTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTACTGCACCAGCGCCAGATGGCTCTACATATTGTCGGCAGATTTACCGATTGGCt                                                        >  1:4956247/1‑93 (MQ=255)

                                  tttttGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCtt                                                       >  2:4508787/1‑93 (MQ=255)

                                  tttttGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCtt                                                       >  2:5310706/1‑93 (MQ=255)

                                   ttttGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTg                                                      >  2:2143045/1‑93 (MQ=255)

                                    tttGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGc                                                     <  2:3321471/93‑1 (MQ=255)

                                     ttGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCt                                                    <  2:2347031/93‑1 (MQ=255)

                                          cgTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGa                                               >  2:6382980/1‑93 (MQ=255)

                                           gttgttCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGaa                                              >  1:808154/1‑93 (MQ=255)

                                            ttgttCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAAc                                             <  1:1298898/93‑1 (MQ=255)

                                             tgttCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACt                                            <  2:723931/93‑1 (MQ=255)

                                             tgttCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACt                                            <  2:6318818/93‑1 (MQ=255)

                                             tgttCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACt                                            <  2:3855682/93‑1 (MQ=255)

                                                tCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGc                                         <  1:1566103/93‑1 (MQ=255)

                                                 caccacCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCg                                        >  2:4562100/1‑93 (MQ=255)

                                                 caccacCTGCACGGTCATATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCg                                        <  2:3550611/93‑1 (MQ=255)

                                                   ccaccTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCgtg                                      >  2:2437505/1‑93 (MQ=255)

                                                     accTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGtgtg                                    >  2:3212408/1‑93 (MQ=255)

                                                     accTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGtgtg                                    <  2:4299345/93‑1 (MQ=255)

                                                      ccTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTgg                                   <  2:359287/93‑1 (MQ=255)

                                                      ccTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTgg                                   >  2:1895241/1‑93 (MQ=255)

                                                       cTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGc                                  >  2:3681565/1‑93 (MQ=255)

                                                        tGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGGAGGCCTGGCGGGTGGCa                                 >  1:2413664/1‑93 (MQ=255)

                                                        tGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCa                                 >  2:2966750/1‑93 (MQ=255)

                                                         gCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGGGGGGCAt                                >  2:539542/1‑93 (MQ=255)

                                                           aCGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCg                              <  2:3457427/93‑1 (MQ=255)

                                                           aCGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCg                              <  1:1183771/93‑1 (MQ=255)

                                                            cGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCgc                             >  1:6233414/1‑92 (MQ=255)

                                                             ggTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTg                            >  1:3302554/1‑93 (MQ=255)

                                                              gTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGa                           <  1:1409/93‑1 (MQ=255)

                                                               tCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTgtt                          <  1:5117701/93‑3 (MQ=255)

                                                               tCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGAt                          <  1:649028/93‑1 (MQ=255)

                                                                cGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATc                         >  1:2181574/1‑93 (MQ=255)

                                                                 ggATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCg                        >  1:87679/1‑93 (MQ=255)

                                                                 ggATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCAACGTGATCg                        <  2:4094329/93‑1 (MQ=255)

                                                                  gATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCgg                       >  1:3089359/1‑93 (MQ=255)

                                                                   aTCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGc                      <  2:5690640/93‑1 (MQ=255)

                                                                   aTCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGc                      <  2:4972959/93‑1 (MQ=255)

                                                                    tCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTTGCGTGTGGGATCGTGATCGGCt                     >  2:697542/1‑93 (MQ=255)

                                                                    tCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCt                     >  2:6438041/1‑93 (MQ=255)

                                                                     cGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCtt                    <  2:3786605/93‑1 (MQ=255)

                                                                      gTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCttt                   <  1:2920905/93‑1 (MQ=255)

                                                                       tCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTg                  <  2:5950298/93‑1 (MQ=255)

                                                                         cTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAg                >  2:1188910/1‑93 (MQ=255)

                                                                         cTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAg                >  1:825097/1‑93 (MQ=255)

                                                                          tGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAgt               <  1:5605834/93‑1 (MQ=255)

                                                                           gCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAgtg              <  1:704801/93‑1 (MQ=255)

                                                                            cACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAgtgt             <  2:3913284/93‑1 (MQ=255)

                                                                             aCCAGCGCCAGCTGGCTATACATATTGTCGGCAGAGTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAGTGtt            <  2:2080880/93‑1 (MQ=255)

                                                                             aCCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAGTGtt            <  2:2899410/93‑1 (MQ=255)

                                                                             aCCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAGTGtt            >  1:922950/1‑93 (MQ=255)

                                                                              ccAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAGTGttt           >  1:4463523/1‑93 (MQ=255)

                                                                              ccAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAGTGttt           <  2:846674/93‑1 (MQ=255)

                                                                               cAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAGTGtttt          >  1:1255268/1‑92 (MQ=255)

                                                                                 gcgcCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAGTGTTTCTa        >  1:4911176/1‑93 (MQ=255)

                                                                                   gcCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAGTGTTTCTAcc      <  1:5478016/93‑1 (MQ=255)

                                                                                    ccAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAGTGTTTCTACCa     >  2:5783069/1‑93 (MQ=255)

                                                                                    ccAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAGTGTTTCTACCa     <  2:4213768/93‑1 (MQ=255)

                                                                                      aGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAGTGTTTCTACCAGc   >  1:3804188/1‑93 (MQ=255)

                                                                                      aGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAGTGTTTCTACCAGc   >  1:1766059/1‑93 (MQ=255)

                                                                                      aGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAGTGTTTCTACCAGc   >  1:5697281/1‑93 (MQ=255)

                                                                                      aGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAGTGTTTCTACCAGc   >  1:6090220/1‑93 (MQ=255)

                                                                                      aGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAGTGTTTCTACCAGc   <  2:2132221/93‑1 (MQ=255)

                                                                                       gATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAGTGTTTCTACCAGCt  <  1:2325997/93‑1 (MQ=255)

                                                                                       gATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAGTGTTTCTACCAGCt  >  1:1681381/1‑93 (MQ=255)

CCGGATCGCCCTGAATGTAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATAGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAGTGTTTCTACCAGCT  >  NC_000913/4432958‑4433137

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GATCCGGATCGCCCTGAATGTAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATAGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAGTGTTTCTACCAGCTTGCT  >  NC_000913/4432955‑4433141

GATCCGGATCGCCCTGAATGTAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATG                                                                                               >  SRR4302534.1081408/1‑94 (MQ=60)

GATCCGGATCGCCCTGAATGTAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATG                                                                                               >  SRR4302534.2296423/1‑94 (MQ=60)

GATCCGGATCGCCCTGAATGTAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATG                                                                                               >  SRR4302534.2810026/1‑94 (MQ=60)

GATCCGGATCGCCCTGAATGTAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATG                                                                                               >  SRR4302534.5145104/1‑94 (MQ=60)

 ATCCGGATCGCCCTGAATGTAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGG                                                                                              >  SRR4302534.182767/1‑94 (MQ=60)

 ATCCGGATCGCCCTGAATGTAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGG                                                                                              <  SRR4302534.1777488/94‑1 (MQ=60)

  TCCGGATCGCCCTGAATGTAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGC                                                                                             >  SRR4302534.5572836/1‑94 (MQ=60)

  TCCGGATCGCCCTGAATGTAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGC                                                                                             <  SRR4302534.4844498/94‑1 (MQ=60)

    CGGATCGCCCTGAATGTAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTA                                                                                           <  SRR4302534.5571599/94‑1 (MQ=60)

    CGGATCGCCCTGAATGTAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTA                                                                                           <  SRR4302534.3161193/94‑1 (MQ=60)

    CGGATCGCCCTGAATGTAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTA                                                                                           >  SRR4302534.706073/1‑94 (MQ=60)

     GGATCGCCCTGAATGTAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTAT                                                                                          >  SRR4302534.6353783/1‑94 (MQ=60)

     GGATCGCCCTGAATGTAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTAT                                                                                          <  SRR4302534.2457065/94‑1 (MQ=60)

     GGATCGCCCTGAATGTAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTAT                                                                                          >  SRR4302534.6029106/1‑94 (MQ=60)

      GATCGCCCTGAATGTAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATA                                                                                         >  SRR4302534.5081107/1‑94 (MQ=60)

      GATCGCCCTGAATGTAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATA                                                                                         <  SRR4302534.2494811/94‑1 (MQ=60)

      GATCGCCCTGAATGTAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATA                                                                                         >  SRR4302534.5449704/1‑94 (MQ=60)

       ATCGCCCTGAATGTAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATAC                                                                                        <  SRR4302534.3085026/94‑1 (MQ=60)

        TCGCCCTGAATGTAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACA                                                                                       >  SRR4302534.156184/1‑94 (MQ=60)

         gtCCCTGAATATAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACAT                                                                                      <  SRR4302534.6193032/92‑1 (MQ=60)

           CCCTGAATGTAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATAT                                                                                    >  SRR4302534.5301843/1‑94 (MQ=60)

           CCCTGAATGTAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATAAATAT                                                                                    >  SRR4302534.3940260/1‑94 (MQ=60)

            CCTGATTGTAATGCTCGACATACGCTTTTTGCGCGTTGTACCCCACCAGCTCGGTCGGAACGTCCTGCACCAGCGCCAGATGGCTCTACATATT                                                                                   <  SRR4302534.6651883/94‑1 (MQ=60)

            CCTGAATGTAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATT                                                                                   >  SRR4302534.6188307/1‑94 (MQ=60)

             CTGAATGTAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTG                                                                                  >  SRR4302534.4999519/1‑94 (MQ=60)

             CTGAATGTAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTG                                                                                  >  SRR4302534.5058489/1‑94 (MQ=60)

             CTGAATGTAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTG                                                                                  >  SRR4302534.6202854/1‑94 (MQ=60)

             CTAAATGTAATGCTCGACATGCGCTTTTTGCGCGTTGTTCACCACCTGCTCGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTG                                                                                  <  SRR4302534.3536875/94‑1 (MQ=60)

              TGAATGTAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGT                                                                                 >  SRR4302534.1219730/1‑94 (MQ=60)

               GAATGTAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTC                                                                                >  SRR4302534.2604512/1‑94 (MQ=60)

               GAATGTAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTC                                                                                >  SRR4302534.4379080/1‑94 (MQ=60)

                AATGTAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCG                                                                               <  SRR4302534.5986547/94‑1 (MQ=60)

                AATGTAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCG                                                                               >  SRR4302534.5169534/1‑94 (MQ=60)

                AATGTAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCG                                                                               <  SRR4302534.4867157/94‑1 (MQ=60)

                 ATGTAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTCCACCAGCGCCGGATGGCTATCCATATTTTCGG                                                                              >  SRR4302534.6331496/1‑94 (MQ=60)

                 ATGTAATGCTAGACATACGCTTTTTGCGCGTTGTTCACCCCCTGCCCGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGG                                                                              <  SRR4302534.853733/94‑1 (MQ=60)

                  TGTAATGATCGACATACGCTTTTTGCGCGTTGTTCACCACCTGAACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGC                                                                             >  SRR4302534.3567825/1‑94 (MQ=60)

                   GTAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCA                                                                            <  SRR4302534.3647171/94‑1 (MQ=60)

                    TAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAG                                                                           >  SRR4302534.3437429/1‑94 (MQ=60)

                    TAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAG                                                                           >  SRR4302534.5077589/1‑94 (MQ=60)

                     AATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGA                                                                          <  SRR4302534.288829/94‑1 (MQ=60)

                     AATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGA                                                                          >  SRR4302534.3140198/1‑94 (MQ=60)

                     AATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGA                                                                          >  SRR4302534.917400/1‑94 (MQ=60)

                       TGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATT                                                                        >  SRR4302534.4066932/1‑94 (MQ=60)

                       TGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCCGCAGCTT                                                                        >  SRR4302534.2681659/1‑94 (MQ=60)

                         CTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGGTTTA                                                                      >  SRR4302534.2706506/1‑94 (MQ=60)

                         CTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTA                                                                      >  SRR4302534.471810/1‑94 (MQ=60)

                         CTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTA                                                                      >  SRR4302534.2964465/1‑94 (MQ=60)

                         CTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTA                                                                      >  SRR4302534.3224309/1‑94 (MQ=60)

                         CTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTA                                                                      <  SRR4302534.74564/94‑1 (MQ=60)

                          TCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTAC                                                                     >  SRR4302534.611603/1‑94 (MQ=60)

                          TCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTAC                                                                     <  SRR4302534.4611418/94‑1 (MQ=60)

                           CGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACC                                                                    <  SRR4302534.506888/94‑1 (MQ=60)

                           CGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACC                                                                    <  SRR4302534.2042101/94‑1 (MQ=60)

                            GACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCG                                                                   <  SRR4302534.3784486/94‑1 (MQ=60)

                            GACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCG                                                                   <  SRR4302534.5771337/94‑1 (MQ=60)

                             ACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGA                                                                  >  SRR4302534.403254/1‑94 (MQ=60)

                             ACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGA                                                                  <  SRR4302534.1664416/94‑1 (MQ=60)

                             ACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGA                                                                  >  SRR4302534.427544/1‑94 (MQ=60)

                             ACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGA                                                                  >  SRR4302534.3603292/1‑94 (MQ=60)

                              CATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGAT                                                                 <  SRR4302534.1633427/94‑1 (MQ=60)

                              CATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTACACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGAT                                                                 >  SRR4302534.1093595/1‑94 (MQ=60)

                                TACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTG                                                               <  SRR4302534.5629237/94‑1 (MQ=60)

                                TACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTG                                                               <  SRR4302534.40659/94‑1 (MQ=60)

                                TACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTG                                                               <  SRR4302534.2305318/94‑1 (MQ=60)

                                 ACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGG                                                              <  SRR4302534.3958621/94‑1 (MQ=60)

                                 ACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGG                                                              >  SRR4302534.4215980/1‑94 (MQ=60)

                                   GCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGACCGCTATACATATTGTCGGCAGATTTACCGATTGCCT                                                            >  SRR4302534.2684142/1‑94 (MQ=60)

                                   GCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTACTGCACCAGCGCCAGATGGCTCTACATATTGTCGGCAGATTTACCGATTGGCT                                                            >  SRR4302534.5090464/1‑94 (MQ=60)

                                    CTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTT                                                           <  SRR4302534.4762715/94‑1 (MQ=60)

                                     TTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTG                                                          <  SRR4302534.1922809/94‑1 (MQ=60)

                                     TTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTG                                                          <  SRR4302534.1808457/94‑1 (MQ=60)

                                       TTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCT                                                        <  SRR4302534.1173027/94‑1 (MQ=60)

                                       TTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCT                                                        >  SRR4302534.3851604/1‑94 (MQ=60)

                                       TTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCT                                                        <  SRR4302534.5709061/94‑1 (MQ=60)

                                        TTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTG                                                       <  SRR4302534.6575840/94‑1 (MQ=60)

                                         TGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGA                                                      <  SRR4302534.1362321/94‑1 (MQ=60)

                                         TGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGA                                                      <  SRR4302534.1817693/94‑1 (MQ=60)

                                          GCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGAC                                                     <  SRR4302534.2914335/94‑1 (MQ=60)

                                             CGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAA                                                  >  SRR4302534.828465/1‑94 (MQ=60)

                                               TTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACT                                                >  SRR4302534.5300370/1‑94 (MQ=60)

                                               TTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACT                                                <  SRR4302534.3002377/94‑1 (MQ=60)

                                               TTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACT                                                <  SRR4302534.1333140/94‑1 (MQ=60)

                                                TGTTCCCCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTG                                               <  SRR4302534.1233796/94‑1 (MQ=60)

                                                TGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTG                                               <  SRR4302534.2972973/94‑1 (MQ=60)

                                                TGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTG                                               >  SRR4302534.5354381/1‑94 (MQ=60)

                                                 GTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGGTGACGACCTGG                                              >  SRR4302534.347943/1‑94 (MQ=60)

                                                 GTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGG                                              >  SRR4302534.292513/1‑94 (MQ=60)

                                                 GTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGG                                              <  SRR4302534.2853736/94‑1 (MQ=60)

                                                   TCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCG                                            <  SRR4302534.1607115/94‑1 (MQ=60)

                                                    CACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGT                                           >  SRR4302534.845564/1‑94 (MQ=60)

                                                    CACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGG                                           >  SRR4302534.131052/1‑94 (MQ=60)

                                                       CACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTG                                        <  SRR4302534.7250/94‑1 (MQ=60)

                                                          CTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGgaggcctggcgggtggca                                     >  SRR4302534.2477858/1‑76 (MQ=60)

                                                          CTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCA                                     >  SRR4302534.574695/1‑94 (MQ=60)

                                                           TGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCAT                                    >  SRR4302534.1218652/1‑94 (MQ=60)

                                                           TGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCAT                                    <  SRR4302534.2367471/94‑1 (MQ=60)

                                                           TGCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCAT                                    >  SRR4302534.5846166/1‑94 (MQ=60)

                                                            GCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATC                                   <  SRR4302534.1441599/94‑1 (MQ=60)

                                                            GCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATC                                   <  SRR4302534.3364338/94‑1 (MQ=60)

                                                            GCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATC                                   <  SRR4302534.4883100/94‑1 (MQ=60)

                                                            GCACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATC                                   >  SRR4302534.6384559/1‑94 (MQ=60)

                                                             CACGGTCGTACAGTCCTTCACCAGCTCAAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGACGTGTGGCATCG                                  <  SRR4302534.5796038/94‑1 (MQ=60)

                                                             CACGGTCGGATCGTCCTGCACCAGCGCCCGATGGCTATACATATTGTCGGCAGATTTACCGATTAGCTTGCTGACGAACTGGCGggtgtactcg                                  >  SRR4302534.275240/1‑84 (MQ=60)

                                                              ACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGT                                 <  SRR4302534.1214882/94‑1 (MQ=60)

                                                              ACGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGC                                 >  SRR4302534.6402603/1‑94 (MQ=60)

                                                               CGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTG                                <  SRR4302534.5194819/94‑1 (MQ=60)

                                                               CGGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTG                                >  SRR4302534.3390715/1‑94 (MQ=60)

                                                                GGTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGGACTGGCGGGTTGCCTCGTGA                               >  SRR4302534.4617092/1‑94 (MQ=60)

                                                                 GTCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGAT                              <  SRR4302534.1447/94‑1 (MQ=60)

                                                                  TCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGTTC                             <  SRR4302534.5255844/94‑1 (MQ=60)

                                                                  TCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATC                             <  SRR4302534.6187243/94‑1 (MQ=60)

                                                                  TCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATC                             >  SRR4302534.2239240/1‑94 (MQ=60)

                                                                  TCGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATC                             <  SRR4302534.664769/94‑1 (MQ=60)

                                                                   CGGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCG                            >  SRR4302534.90022/1‑94 (MQ=60)

                                                                   CGGATCATCCTGCACCAGCACCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCG                            <  SRR4302534.6079408/94‑1 (MQ=60)

                                                                    GGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGG                           >  SRR4302534.3170585/1‑94 (MQ=60)

                                                                    GGATCGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGG                           >  SRR4302534.3601874/1‑94 (MQ=60)

                                                                        CGTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTT                       <  SRR4302534.3624031/94‑1 (MQ=60)

                                                                         GTCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTG                      <  SRR4302534.2997561/94‑1 (MQ=60)

                                                                          TCCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGA                     >  SRR4302534.5974427/1‑94 (MQ=60)

                                                                           CCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAG                    >  SRR4302534.845859/1‑94 (MQ=60)

                                                                           CCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAG                    <  SRR4302534.862575/94‑1 (MQ=60)

                                                                           CCTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAG                    <  SRR4302534.3483037/94‑1 (MQ=60)

                                                                            CTGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTTAGT                   >  SRR4302534.2207729/1‑94 (MQ=60)

                                                                             TGCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAGTG                  <  SRR4302534.5757465/94‑1 (MQ=60)

                                                                              GCACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAGTGT                 <  SRR4302534.722087/94‑1 (MQ=60)

                                                                               CACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAGTGTT                >  SRR4302534.946457/1‑94 (MQ=60)

                                                                                ACCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAGTGTTT               >  SRR4302534.4584215/1‑94 (MQ=60)

                                                                                 CCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAGTGTTTT              >  SRR4302534.1288348/1‑94 (MQ=60)

                                                                                 CCAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAGTGTTTC              >  SRR4302534.6317817/1‑94 (MQ=60)

                                                                                  CAGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAGTGTTTCT             >  SRR4302534.939239/1‑94 (MQ=60)

                                                                                   AGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAGTGTTTCTA            >  SRR4302534.5044280/1‑94 (MQ=60)

                                                                                   AGCGCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAGTGTTTCTA            <  SRR4302534.2461609/94‑1 (MQ=60)

                                                                                      GCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAGTGTTTCTACCA         >  SRR4302534.5804614/1‑94 (MQ=60)

                                                                                      GCCAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAGTGTTTCTACCA         <  SRR4302534.5625725/94‑1 (MQ=60)

                                                                                        CAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAGTGTTTCTACCAGC       >  SRR4302534.5851615/1‑94 (MQ=60)

                                                                                        CAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAGTGTTTCTACCAGC       >  SRR4302534.1811922/1‑94 (MQ=60)

                                                                                        CAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAGTGTTTCTACCAGC       >  SRR4302534.6255844/1‑94 (MQ=60)

                                                                                        CAGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAGTGTTTCTACCAGC       >  SRR4302534.3906334/1‑94 (MQ=60)

                                                                                         AGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAGTGTTTCTACCAGCT      >  SRR4302534.4050880/1‑94 (MQ=60)

                                                                                         AGATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAGTGTTTCTACCAGCT      >  SRR4302534.1725118/1‑94 (MQ=60)

                                                                                          GATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAGTGTTTCTACCAGCTT     <  SRR4302534.2387719/94‑1 (MQ=60)

                                                                                           ATGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAGTGTTTCTACCAGCTTG    <  SRR4302534.3071519/94‑1 (MQ=60)

                                                                                            TGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAGTGTTTCTACCAGCTTGC   <  SRR4302534.1898933/94‑1 (MQ=60)

                                                                                            TGGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAGTGTTTCTACCAGCTTGC   >  SRR4302534.1037279/1‑94 (MQ=60)

                                                                                            TGGCTATACATATTGGCGGCAGATTTACCGATTTGCTTGCTGACGAACTGGCGTGTGGCATCGGGATGGGCTTTGAGGGTTTCTtccgccttgt   >  SRR4302534.6105111/1‑84 (MQ=60)

                                                                                             GGCTATACATATTGTCGTCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAGTGTTTCTACCAGCTTGCT  <  SRR4302534.6568008/94‑1 (MQ=60)

                                                                                             GGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAGTGTTTCTACCAGCTTGCT  <  SRR4302534.4843883/94‑1 (MQ=60)

                                                                                             GGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAGTGTTTCTACCAGCTTGCT  >  SRR4302534.519090/1‑94 (MQ=60)

                                                                                             GGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAGTGTTTCTACCAGCTTGCT  <  SRR4302534.166240/94‑1 (MQ=60)

GATCCGGATCGCCCTGAATGTAATGCTCGACATACGCTTTTTGCGCGTTGTTCACCACCTGCACGGTCGGATCGTCCTGCACCAGCGCCAGATAGCTATACATATTGTCGGCAGATTTACCGATTGGCTTGCTGACGAACTGGCGTGTGGCATCGTGATCGGCTTTGAGTGTTTCTACCAGCTTGCT  >  NC_000913/4432955‑4433141

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`