Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 744 338.8 17650688 97.8% 17262372 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,477,321 Δ1 bp coding (12/1182 nt) yjgN → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,477,3210A.99.7% 1354.6 / ‑3.2 293coding (12/1182 nt)yjgNhypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base . (175/116);  minor base C (0/1);  total (175/118)
Fisher's exact test for biased strand distribution p-value = 4.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 293 total aligned reads displayed.

AACATTTTACTGTCTGTATATATAAGTTATATCAATGGATTTTTTAATTTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGATAGTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATAACG  >  NC_000913/4477232‑4477408
                                                                                         |                                                                                       
aaCATTTTACTGTCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTcc                                                                                    <  1:6488954/93‑1 (MQ=255)
aaCATTTTACTGTCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTcc                                                                                    >  1:1635029/1‑93 (MQ=255)
  cATTTTACTGTCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGt                                                                                  >  1:7992343/1‑93 (MQ=255)
  cATTTTACTGTCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGt                                                                                  >  1:2533241/1‑93 (MQ=255)
   aTTTTACTGTCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTc                                                                                 <  2:8021287/93‑1 (MQ=255)
    ttttACTGTCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTcc                                                                                >  2:7100111/1‑93 (MQ=255)
     tttAATGTCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTccc                                                                               <  2:2420732/93‑1 (MQ=255)
      ttACTGTCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCa                                                                              <  1:3090817/93‑1 (MQ=255)
         ctgtctgtATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTc                                                                           >  1:7290990/1‑93 (MQ=255)
          tgtctgtATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCAttag                                                                          >  1:3408685/1‑91 (MQ=255)
          tgtctgtATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCg                                                                          <  2:5694038/93‑1 (MQ=255)
           gtctgtATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCgt                                                                         >  2:925831/1‑93 (MQ=255)
             ctgtATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCgttt                                                                       >  1:4520505/1‑93 (MQ=255)
              tgtATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCgtttg                                                                      >  2:3474850/1‑93 (MQ=255)
               gtATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCgtttgt                                                                     >  2:5098093/1‑93 (MQ=255)
               gtATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCgtttgt                                                                     >  2:7400038/1‑93 (MQ=255)
               gtATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCgtttgt                                                                     >  1:1069976/1‑93 (MQ=255)
                tatatataAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGACATGGAT‑GTTCCGTCCCATTCgtttgtt                                                                    >  1:3860135/1‑93 (MQ=255)
                  tatataAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGtttt                                                                  <  2:5551592/93‑1 (MQ=255)
                  tatataAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGtttt                                                                  >  2:8514062/1‑93 (MQ=255)
                     ataAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCa                                                               <  1:747800/93‑1 (MQ=255)
                     ataAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCa                                                               >  1:3665653/1‑93 (MQ=255)
                      taAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCAt                                                              <  1:5000332/93‑1 (MQ=255)
                        aGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATgg                                                            <  2:6622258/93‑1 (MQ=255)
                         gTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGt                                                           >  2:4601044/1‑93 (MQ=255)
                         gTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGt                                                           >  2:1137081/1‑93 (MQ=255)
                         gTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGt                                                           >  2:5961180/1‑93 (MQ=255)
                          ttATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTa                                                          <  2:890693/93‑1 (MQ=255)
                            atatCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACa                                                        <  2:5055648/93‑1 (MQ=255)
                             tatCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAg                                                       <  1:614467/93‑1 (MQ=255)
                             tatCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAg                                                       >  1:8097426/1‑93 (MQ=255)
                               tCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGt                                                     <  2:1454529/93‑1 (MQ=255)
                               tCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGt                                                     >  2:2103443/1‑93 (MQ=255)
                                cAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTg                                                    >  2:324431/1‑93 (MQ=255)
                                 aaTGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGGAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTga                                                   >  1:183370/1‑93 (MQ=255)
                                 aaTGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTGCGTCCCATTCGTTTGTTTTTCATGGTACAGGTga                                                   <  2:7852674/93‑1 (MQ=255)
                                 aaTGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTga                                                   <  2:3925034/93‑1 (MQ=255)
                                 aaTGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTga                                                   <  2:5370017/93‑1 (MQ=255)
                                   tGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTgaga                                                 <  2:5731203/93‑1 (MQ=255)
                                   tGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTgaga                                                 <  1:5648422/93‑1 (MQ=255)
                                   tGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTgaga                                                 >  1:481227/1‑93 (MQ=255)
                                     gATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTgagaga                                               >  2:4194885/1‑93 (MQ=255)
                                     gATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTgagaga                                               <  1:3671976/93‑1 (MQ=255)
                                     gATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTgagaga                                               >  2:1245603/1‑93 (MQ=255)
                                      aTTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGat                                              <  2:8444991/93‑1 (MQ=255)
                                         ttttAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATAtt                                           >  2:2489328/1‑93 (MQ=255)
                                          tttAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATAttt                                          >  2:2840983/1‑93 (MQ=255)
                                          tttAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATAttt                                          <  2:3685576/93‑1 (MQ=255)
                                            ttaattCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATAtttt                                         <  2:4442407/89‑1 (MQ=255)
                                            ttaattCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATAtttt                                         >  2:7430165/5‑93 (MQ=255)
                                            ttaattCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGA‑CGTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATAtttt                                         <  2:7529369/89‑1 (MQ=255)
                                             taattCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTc                                        >  1:5819136/4‑93 (MQ=255)
                                              aattCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCt                                       <  1:5531492/91‑1 (MQ=255)
                                               attCAGGGAATTTTTATGGCTCACGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCtt                                      <  2:4786318/92‑1 (MQ=255)
                                               attCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCtt                                      <  1:2180234/92‑1 (MQ=255)
                                               attCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCtt                                      >  1:1716151/2‑93 (MQ=255)
                                                ttCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCttt                                     >  1:5899261/1‑92 (MQ=255)
                                                ttCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTa                                     >  2:3004919/1‑93 (MQ=255)
                                                 tCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTAt                                    >  1:8239454/1‑93 (MQ=255)
                                                   aGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTAttt                                  >  1:5277882/1‑93 (MQ=255)
                                                   aGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTAttt                                  <  2:7358998/93‑1 (MQ=255)
                                                    gggAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTtg                                 <  2:6400899/93‑1 (MQ=255)
                                                    gggAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTtg                                 <  2:6486494/93‑1 (MQ=255)
                                                    gggAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTtg                                 >  1:1153153/1‑93 (MQ=255)
                                                    gggAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTtg                                 >  1:6060318/1‑93 (MQ=255)
                                                    gggAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGGGAGAGATATTTTCTTATTtg                                 >  2:2207517/1‑93 (MQ=255)
                                                     ggAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTtgt                                >  1:8602861/1‑93 (MQ=255)
                                                      gAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGATTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTtgtg                               >  2:8309431/1‑93 (MQ=255)
                                                        aTTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTtgtgtg                             <  1:6820152/93‑1 (MQ=255)
                                                        aTTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTtgtgtg                             <  1:5958509/93‑1 (MQ=255)
                                                        aTTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTtgtgtg                             >  1:7575718/1‑93 (MQ=255)
                                                        aTTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGACTTTTTCTTATTtgtgtg                             >  1:7829594/1‑93 (MQ=255)
                                                         tttttATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTgtgg                            >  1:2724581/1‑93 (MQ=255)
                                                         tttttATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTgtgg                            >  1:8451687/1‑93 (MQ=255)
                                                             tATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGaa                        >  2:7284881/1‑93 (MQ=255)
                                                              aTGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAAt                       <  1:4126218/93‑1 (MQ=255)
                                                              aTGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAAt                       >  2:6304911/1‑93 (MQ=255)
                                                                tgCTTAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAAtgt                     <  1:2867005/92‑1 (MQ=255)
                                                                ggCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAAtgt                     >  1:8746712/1‑93 (MQ=255)
                                                                ggCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAAtgt                     <  1:5045353/93‑1 (MQ=255)
                                                                 gCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAAtgtg                    <  2:4951510/93‑1 (MQ=255)
                                                                  cTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATgtgt                   >  1:6596271/1‑93 (MQ=255)
                                                                   tCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGtgtt                  >  2:7776461/1‑93 (MQ=255)
                                                                   tCAAGTTATTAATAAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGtgtt                  >  2:2631794/1‑93 (MQ=255)
                                                                     aaGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGtgttgt                <  1:3477764/93‑1 (MQ=255)
                                                                      aGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTgttgtt               <  1:1393078/93‑1 (MQ=255)
                                                                         tattaATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAAc            <  2:8228224/93‑1 (MQ=255)
                                                                           ttaATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGa          >  2:1566694/1‑93 (MQ=255)
                                                                            taATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGAt         >  1:4205822/1‑93 (MQ=255)
                                                                            taATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGAt         >  2:1824305/1‑93 (MQ=255)
                                                                             aaTGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGAtt        >  1:5184513/1‑93 (MQ=255)
                                                                             aaTGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGAtt        <  2:6179776/93‑1 (MQ=255)
                                                                              aTGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATta       >  2:4415859/1‑93 (MQ=255)
                                                                              aTGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATta       >  2:2965283/1‑93 (MQ=255)
                                                                              aTGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATta       >  2:3834829/1‑93 (MQ=255)
                                                                                gAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATtata     <  2:7726461/93‑1 (MQ=255)
                                                                                gAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATtata     <  2:1635029/93‑1 (MQ=255)
                                                                                  aaTGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATAAc   <  1:7090130/93‑1 (MQ=255)
                                                                                  aaTGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATAAc   <  2:4451478/93‑1 (MQ=255)
                                                                                   aTGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATAACg  >  1:6391553/1‑93 (MQ=255)
                                                                                         |                                                                                       
AACATTTTACTGTCTGTATATATAAGTTATATCAATGGATTTTTTAATTTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGATAGTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATAACG  >  NC_000913/4477232‑4477408

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CAGAACATTTTACTGTCTGTATATATAAGTTATATCAATGGATTTTTTAATTTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGATAGTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATAACGCTAGGTA  >  NC_000913/4477229‑4477415
                                                                                            |                                                                                              
CAGAACATTTTACTGTCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTT                                                                                             <  SRR4302541.567134/94‑1 (MQ=60)
CAGAACATTTTACTGTCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTT                                                                                             >  SRR4302541.4712389/1‑94 (MQ=60)
CAGAACATTTTACTGTCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTT                                                                                             <  SRR4302541.4708288/94‑1 (MQ=60)
CAGAACATTTTACTGTCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTT                                                                                             >  SRR4302541.4261141/1‑94 (MQ=60)
CAGAACATTTTACTGTCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTT                                                                                             >  SRR4302541.5979830/1‑94 (MQ=60)
CAGAACATTTTACTGTCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTT                                                                                             >  SRR4302541.7172463/1‑94 (MQ=60)
 AGAACATTTTACTGTCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTC                                                                                            >  SRR4302541.5326966/1‑94 (MQ=60)
 AGAACATTTTACTGTCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTC                                                                                            >  SRR4302541.4352211/1‑94 (MQ=60)
  GAACATTTTACTGTCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCC                                                                                           >  SRR4302541.1651204/1‑94 (MQ=60)
   AACATTTTACTGTCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCG                                                                                          >  SRR4302541.5790897/1‑94 (MQ=60)
   AACATTTTACTGTCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCG                                                                                          <  SRR4302541.6555097/94‑1 (MQ=60)
   AACATTTTAATGTCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCG                                                                                          >  SRR4302541.109239/1‑94 (MQ=60)
    ACATTTTACTGTCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGT                                                                                         >  SRR4302541.3975338/1‑94 (MQ=60)
    ACATTTTACTGTCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGT                                                                                         >  SRR4302541.2558690/1‑94 (MQ=60)
    ACATTTTACTGTCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGT                                                                                         >  SRR4302541.8080331/1‑94 (MQ=60)
      ATTTTACTGTCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGGT‑GTTCCGTCC                                                                                       >  SRR4302541.3532451/1‑94 (MQ=60)
       TTTTACTGTCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCC                                                                                      <  SRR4302541.8292451/94‑1 (MQ=60)
        TTTACTGTCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCA                                                                                     <  SRR4302541.8815363/94‑1 (MQ=60)
         TTACTGTCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCAT                                                                                    <  SRR4302541.3122164/94‑1 (MQ=60)
           ACTGTCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTC                                                                                  <  SRR4302541.8349541/94‑1 (MQ=60)
           ACTGTCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTC                                                                                  >  SRR4302541.7368815/1‑94 (MQ=60)
            CTGTCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCG                                                                                 >  SRR4302541.3112341/1‑94 (MQ=60)
            CTGTCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCG                                                                                 >  SRR4302541.5341477/1‑94 (MQ=60)
            CTGTCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTAG                                                                                 >  SRR4302541.3442708/1‑94 (MQ=60)
               TCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTT                                                                              >  SRR4302541.4566126/1‑94 (MQ=60)
               TCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTT                                                                              <  SRR4302541.6025897/94‑1 (MQ=60)
                 TGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGT                                                                            >  SRR4302541.1079934/1‑94 (MQ=60)
                  GTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGACATGGAT‑GTTCCGTCCCATTCGTTTGTT                                                                           >  SRR4302541.3898965/1‑94 (MQ=60)
                    ATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTT                                                                         >  SRR4302541.5979574/1‑94 (MQ=60)
                    ATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTT                                                                         >  SRR4302541.1705569/1‑94 (MQ=60)
                    ATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTT                                                                         >  SRR4302541.2137416/1‑94 (MQ=60)
                     TATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTT                                                                        >  SRR4302541.1812803/1‑94 (MQ=60)
                      ATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTC                                                                       >  SRR4302541.1288823/1‑94 (MQ=60)
                      ATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTC                                                                       >  SRR4302541.3707322/1‑94 (MQ=60)
                      ATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTC                                                                       >  SRR4302541.5612601/1‑94 (MQ=60)
                       TATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCA                                                                      >  SRR4302541.8594905/1‑94 (MQ=60)
                       TATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCA                                                                      >  SRR4302541.3702844/1‑94 (MQ=60)
                       TATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCA                                                                      >  SRR4302541.3702843/1‑94 (MQ=60)
                        ATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCAT                                                                     <  SRR4302541.6165677/94‑1 (MQ=60)
                        ATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCAT                                                                     <  SRR4302541.754879/94‑1 (MQ=60)
                         TAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATG                                                                    >  SRR4302541.5258076/1‑94 (MQ=60)
                         TAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATG                                                                    <  SRR4302541.5051706/94‑1 (MQ=60)
                          AAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGG                                                                   <  SRR4302541.4812636/94‑1 (MQ=60)
                           AGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGT                                                                  <  SRR4302541.2243118/94‑1 (MQ=60)
                           AGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGT                                                                  <  SRR4302541.5175248/94‑1 (MQ=60)
                           AGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGT                                                                  >  SRR4302541.3126278/1‑94 (MQ=60)
                             TTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTCATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTAC                                                                >  SRR4302541.3782192/1‑94 (MQ=60)
                               ATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAG                                                              >  SRR4302541.3485039/1‑94 (MQ=60)
                               ATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAG                                                              >  SRR4302541.8186189/1‑94 (MQ=60)
                               ATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAG                                                              >  SRR4302541.8186191/1‑94 (MQ=60)
                                TATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGG                                                             <  SRR4302541.620229/94‑1 (MQ=60)
                                  TCAATTGATTTTTTAA‑TTCAGGGAATTTTTATGTCTCAAGTTATTAATGTATTGG‑TTGTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTG                                                           <  SRR4302541.1550903/94‑1 (MQ=60)
                                  TCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTG                                                           >  SRR4302541.6618522/1‑94 (MQ=60)
                                   CAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGGAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGA                                                          >  SRR4302541.184745/1‑94 (MQ=60)
                                   CAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGA                                                          >  SRR4302541.346643/1‑94 (MQ=60)
                                    AATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAG                                                         <  SRR4302541.3927572/94‑1 (MQ=60)
                                     ATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGA                                                        >  SRR4302541.485787/1‑94 (MQ=60)
                                      TGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAG                                                       >  SRR4302541.5154761/1‑94 (MQ=60)
                                      TGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAG                                                       <  SRR4302541.5706137/94‑1 (MQ=60)
                                       GGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGA                                                      >  SRR4302541.564482/1‑94 (MQ=60)
                                       GGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGA                                                      >  SRR4302541.6462937/1‑94 (MQ=60)
                                        GATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGAT                                                     <  SRR4302541.3709203/94‑1 (MQ=60)
                                         ATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATTGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATT                                                    >  SRR4302541.5955459/1‑94 (MQ=60)
                                         ATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATA                                                    >  SRR4302541.4239879/1‑94 (MQ=60)
                                             TTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTT                                                >  SRR4302541.7843139/1‑94 (MQ=60)
                                              TTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTC                                               <  SRR4302541.7048466/94‑1 (MQ=60)
                                              TTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTC                                               <  SRR4302541.4967300/94‑1 (MQ=60)
                                              TTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTC                                               >  SRR4302541.5878277/1‑94 (MQ=60)
                                               TAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCT                                              >  SRR4302541.204000/1‑94 (MQ=60)
                                               TAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCT                                              >  SRR4302541.1802602/1‑94 (MQ=60)
                                                 AATTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTT                                             >  SRR4302541.1732939/1‑94 (MQ=60)
                                                 AATTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTT                                             <  SRR4302541.5588116/94‑1 (MQ=60)
                                                  ATTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTT                                            >  SRR4302541.5959299/1‑94 (MQ=60)
                                                  ATTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTA                                            >  SRR4302541.8067185/1‑94 (MQ=60)
                                                  ATTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTA                                            <  SRR4302541.2201303/94‑1 (MQ=60)
                                                   TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTAT                                           >  SRR4302541.8329245/1‑94 (MQ=60)
                                                     CAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTT                                         >  SRR4302541.5906456/1‑94 (MQ=60)
                                                     CAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTT                                         >  SRR4302541.5332230/1‑94 (MQ=60)
                                                     CAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTT                                         >  SRR4302541.5086694/1‑94 (MQ=60)
                                                     CAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGAAATTTTCTTATTT                                         >  SRR4302541.7280002/1‑94 (MQ=60)
                                                      AGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGaccaggggagagtaatttctttttttt                                        >  SRR4302541.8685570/1‑67 (MQ=60)
                                                      AGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTG                                        >  SRR4302541.6122235/1‑94 (MQ=60)
                                                      AGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTG                                        >  SRR4302541.7385987/1‑94 (MQ=60)
                                                      AGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTG                                        >  SRR4302541.1164179/1‑94 (MQ=60)
                                                      AGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTG                                        >  SRR4302541.280673/1‑94 (MQ=60)
                                                       GGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGT                                       >  SRR4302541.8695148/1‑94 (MQ=60)
                                                        GGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTG                                      <  SRR4302541.5362327/94‑1 (MQ=60)
                                                          AATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTG                                    >  SRR4302541.7656666/1‑94 (MQ=60)
                                                          AATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTG                                    >  SRR4302541.7656665/1‑94 (MQ=60)
                                                          AATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGACTTTTTCTTATTTGTGTG                                    >  SRR4302541.7914780/1‑94 (MQ=60)
                                                           ATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGG                                   >  SRR4302541.8542910/1‑94 (MQ=60)
                                                           ATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGG                                   <  SRR4302541.6893136/94‑1 (MQ=60)
                                                           ATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGG                                   >  SRR4302541.4257812/1‑94 (MQ=60)
                                                           ATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGG                                   >  SRR4302541.2751805/1‑94 (MQ=60)
                                                           ATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGG                                   <  SRR4302541.6019242/94‑1 (MQ=60)
                                                           ATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGG                                   <  SRR4302541.6019243/94‑1 (MQ=60)
                                                           ATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGG                                   >  SRR4302541.2751796/1‑94 (MQ=60)
                                                             TTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTG                                 >  SRR4302541.1564155/1‑94 (MQ=60)
                                                              TTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGA                                <  SRR4302541.666069/94‑1 (MQ=60)
                                                                 ATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATG                             <  SRR4302541.4166977/94‑1 (MQ=60)
                                                                 ATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATG                             <  SRR4302541.4166976/94‑1 (MQ=60)
                                                                 ATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATG                             >  SRR4302541.4816640/1‑94 (MQ=60)
                                                                  TGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGCGATATTTTCTTATTTGTGTGGTGAATGT                            >  SRR4302541.6225633/1‑94 (MQ=60)
                                                                  TGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGT                            >  SRR4302541.8162627/1‑94 (MQ=60)
                                                                  TGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGT                            >  SRR4302541.5059802/1‑94 (MQ=60)
                                                                  TGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGT                            >  SRR4302541.8840299/1‑94 (MQ=60)
                                                                   tgcttAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTG                           <  SRR4302541.2895808/89‑1 (MQ=60)
                                                                   GGCTCAAGTTATTAGTGCTATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTG                           <  SRR4302541.7897267/94‑1 (MQ=60)
                                                                   GGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTG                           >  SRR4302541.5281289/1‑94 (MQ=60)
                                                                   GGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTG                           <  SRR4302541.5097270/94‑1 (MQ=60)
                                                                    GCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGT                          >  SRR4302541.6663819/1‑94 (MQ=60)
                                                                    GCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGT                          >  SRR4302541.7611548/1‑94 (MQ=60)
                                                                     CTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTT                         <  SRR4302541.244294/94‑1 (MQ=60)
                                                                      TCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTG                        >  SRR4302541.5864309/1‑94 (MQ=60)
                                                                        AAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTT                      >  SRR4302541.6684648/1‑94 (MQ=60)
                                                                        AAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTT                      <  SRR4302541.3672560/94‑1 (MQ=60)
                                                                        AAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTT                      <  SRR4302541.3512550/94‑1 (MQ=60)
                                                                         AGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGCATGTGTTGTTA                     >  SRR4302541.3238140/1‑94 (MQ=60)
                                                                         AGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTA                     <  SRR4302541.95579/94‑1 (MQ=60)
                                                                         AGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTA                     <  SRR4302541.1406731/94‑1 (MQ=60)
                                                                          GTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAA                    <  SRR4302541.6483931/94‑1 (MQ=60)
                                                                            TATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACG                  >  SRR4302541.951329/1‑94 (MQ=60)
                                                                             ATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGA                 >  SRR4302541.1398017/1‑94 (MQ=60)
                                                                             ATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGA                 >  SRR4302541.2636798/1‑94 (MQ=60)
                                                                             ATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGA                 >  SRR4302541.5045207/1‑94 (MQ=60)
                                                                              TTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGAT                >  SRR4302541.3079790/1‑94 (MQ=60)
                                                                              TTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGAT                >  SRR4302541.6145818/1‑94 (MQ=60)
                                                                              TTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGAT                >  SRR4302541.4247637/1‑94 (MQ=60)
                                                                               TAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATT               >  SRR4302541.6214432/1‑94 (MQ=60)
                                                                               TAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATT               >  SRR4302541.5237850/1‑94 (MQ=60)
                                                                               TAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATT               >  SRR4302541.1651275/1‑94 (MQ=60)
                                                                                AATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTA              <  SRR4302541.2752078/94‑1 (MQ=60)
                                                                                 ATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTAT             <  SRR4302541.8736968/94‑1 (MQ=60)
                                                                                 ATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTAT             <  SRR4302541.7170313/94‑1 (MQ=60)
                                                                                  TGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATA            <  SRR4302541.6572145/94‑1 (MQ=60)
                                                                                  TGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATA            <  SRR4302541.7139191/94‑1 (MQ=60)
                                                                                   GAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATAA           <  SRR4302541.4348548/94‑1 (MQ=60)
                                                                                    AAATGTAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATAAC          <  SRR4302541.6848430/94‑1 (MQ=60)
                                                                                    AAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATAAC          >  SRR4302541.8285646/1‑94 (MQ=60)
                                                                                    AAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATAAC          <  SRR4302541.7530697/94‑1 (MQ=60)
                                                                                    AAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATAAC          <  SRR4302541.8071010/94‑1 (MQ=60)
                                                                                     AATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATAACG         <  SRR4302541.7165555/94‑1 (MQ=60)
                                                                                     AATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATAACG         >  SRR4302541.6456800/1‑94 (MQ=60)
                                                                                      ATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATAACGC        >  SRR4302541.1687672/1‑94 (MQ=60)
                                                                                      ATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATAACGC        >  SRR4302541.5837483/1‑94 (MQ=60)
                                                                                      ATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATAACGC        >  SRR4302541.1386613/1‑94 (MQ=60)
                                                                                      ATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATAACGC        >  SRR4302541.2141251/1‑94 (MQ=60)
                                                                                         GAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATAACGCTAG     >  SRR4302541.8241656/1‑94 (MQ=60)
                                                                                            TGTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATAACGCTAGGT   >  SRR4302541.583190/1‑94 (MQ=60)
                                                                                            TGTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTAT‑ACGCTAGGTA  <  SRR4302541.5697960/94‑1 (MQ=60)
                                                                                            |                                                                                              
CAGAACATTTTACTGTCTGTATATATAAGTTATATCAATGGATTTTTTAATTTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGATAGTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATAACGCTAGGTA  >  NC_000913/4477229‑4477415

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑