Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 744 338.8 17650688 97.8% 17262372 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,603,663 (G)8→9 noncoding (72/88 nt) leuV ← tRNA‑Leu

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,603,6551.G91.4% 655.9 / 55.3 222noncoding (80/88 nt)leuVtRNA‑Leu
Reads supporting (aligned to +/- strand):  ref base . (11/8);  new base G (134/69);  total (145/77)
Fisher's exact test for biased strand distribution p-value = 4.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 272 total aligned reads displayed.

TTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GA‑‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTAC  >  NC_000913/4603563‑4603747
                                                                                              |                                                                                                
   ttGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GA‑‑‑g‑gg                                                                                            >  1:6246943/1‑93 (MQ=255)
    tGCTTGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GA‑‑‑g‑ggg                                                                                           <  2:4639086/93‑1 (MQ=255)
     gCTGGCATCATGCTGATTATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GA‑‑‑g‑gggg                                                                                          <  1:5530004/93‑1 (MQ=255)
      cttgCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GA‑‑‑g‑ggggg                                                                                         <  2:1972041/90‑1 (MQ=255)
      cTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GA‑‑‑g‑ggggg                                                                                         <  1:2963404/93‑1 (MQ=255)
      cTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GA‑‑‑g‑ggggg                                                                                         <  1:8391873/93‑1 (MQ=255)
      cTGGCATCATGCTGATGATAATGAATATGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GA‑‑‑g‑ggggg                                                                                         <  2:3540544/93‑1 (MQ=255)
       tGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GA‑‑‑g‑gggggg                                                                                        <  2:1326184/93‑1 (MQ=255)
        gggatcatGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GA‑‑‑g‑ggggggg                                                                                       <  2:2937547/90‑1 (MQ=255)
        ggCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GA‑‑‑g‑ggggggg                                                                                       >  1:5931288/1‑93 (MQ=255)
        ggCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GA‑‑‑g‑ggggggg                                                                                       >  2:5247595/1‑93 (MQ=255)
        ggCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GA‑‑‑g‑ggggggg                                                                                       >  2:5126588/1‑93 (MQ=255)
           atcatGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GA‑‑‑g‑ggggggggac                                                                                    >  2:8167804/1‑90 (MQ=255)
            tcatGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GA‑‑‑g‑ggggggggact                                                                                   >  1:485373/1‑89 (MQ=255)
                gCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGaa                                                                               >  1:374799/1‑93 (MQ=255)
                 cTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAAc                                                                              >  1:4269116/1‑93 (MQ=255)
                  tgatgaTAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGTTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAAcc                                                                             >  2:4804535/1‑93 (MQ=255)
                   gatgatAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAAccc                                                                            >  2:3798365/1‑93 (MQ=255)
                        tAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACACCCACg                                                                       >  1:2140156/1‑93 (MQ=255)
                         aaTGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCACgc                                                                      >  2:6492089/1‑92 (MQ=255)
                          aTGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTc                                                                     <  1:7059903/93‑1 (MQ=255)
                          aTGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTc                                                                     >  1:5548005/1‑93 (MQ=255)
                             aaTTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATCATTGGCGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCACACGTCCgc                                                                  >  1:4388981/1‑92 (MQ=38)
                             aaTTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCga                                                                  >  1:5481648/1‑92 (MQ=255)
                              aTTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTa                                                                 >  2:5863620/1‑93 (MQ=255)
                               tttGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GA‑‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAg                                                               >  2:4211055/1‑93 (MQ=255)
                                ttGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAGTTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAg                                                               <  2:6169763/93‑1 (MQ=255)
                                ttGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATGGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAg                                                               <  2:8480613/93‑1 (MQ=255)
                                    acgaGGGATGAAAAAGAAAGAAAGAAACAAGAAAGGAAGTAAAGATAATTGGGGC‑GAG‑‑G‑GGGGGGGAC‑TTGACCCCCCACGTCCGTAAGGaca                                                           <  2:5491287/90‑1 (MQ=25)
                                     tCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGacac                                                          >  2:4600711/1‑93 (MQ=255)
                                     acAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GA‑‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACt                                                         <  1:8685340/92‑1 (MQ=255)
                                        gggATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACTaa                                                       <  1:8615925/93‑1 (MQ=255)
                                          gATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACTAaca                                                     >  2:4327681/1‑93 (MQ=255)
                                           aTGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAGG‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACTAaca                                                     >  1:3869346/1‑93 (MQ=25)
                                           aTGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCATGTCCGTAAGGACACTAacac                                                    >  1:1296333/1‑93 (MQ=255)
                                            tGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGACCCCCCACGTCCGTAAGGACACTAACAcc                                                   <  1:7326806/93‑1 (MQ=255)
                                               aaaaTCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGa                                                >  1:1478666/1‑93 (MQ=255)
                                                  ataaaGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑‑G‑GGGGGGGGC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGc                                             <  2:6051704/90‑1 (MQ=21)
                                                  aTCAAGGAAGAAACAAGAAAGGAAGTAAAGATAGTTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGc                                             >  1:2476045/1‑93 (MQ=255)
                                                   tCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCt                                            >  2:2169204/1‑93 (MQ=255)
                                                   acAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCt                                            <  1:5492529/92‑1 (MQ=255)
                                                   acAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCt                                            <  1:5656249/92‑1 (MQ=255)
                                                    cAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTa                                           >  1:2837141/1‑93 (MQ=255)
                                                     aaGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAg                                          >  2:2447795/1‑93 (MQ=255)
                                                     aaGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAg                                          >  2:7033165/1‑93 (MQ=255)
                                                     aaGGAAGAAACAAGAAAGGAAGTAAAGATAATGGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAg                                          <  1:8105410/93‑1 (MQ=21)
                                                      aGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAgc                                         >  1:2242807/1‑93 (MQ=255)
                                                      aGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAgc                                         >  1:6385064/1‑93 (MQ=255)
                                                       ggAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAgcg                                        >  1:6723610/1‑93 (MQ=255)
                                                       ggAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCAACGCCGTAAGGAGACTAACACCTGAAGCTAccg                                        >  2:4863252/1‑93 (MQ=21)
                                                        gaagaaACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAgcgc                                       >  1:3314335/1‑93 (MQ=255)
                                                          agaaACAAGAAAGGAAGTAAAGATAATTGGTGC‑GA‑‑‑G‑GGGGGGGGCATTGAACCCCCAGTTCCGTCAGGACCCTAAAACCTGAACCTATCGCGt                                     >  2:6130822/1‑93 (MQ=18)
                                                           gaaACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAGG‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGt                                     >  1:881437/1‑93 (MQ=18)
                                                           gaaACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTc                                    <  2:2841577/93‑1 (MQ=255)
                                                           gaaACAAGAAAGGAAGTAAAGATAATTGGTGC‑GA‑‑‑G‑GGGGGGGGC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCt                                   >  1:4711536/1‑93 (MQ=255)
                                                           gaaACAAAAAAGGAATGAAAAATAATTGGTGCTGG‑‑‑G‑GGGGGGGAC‑TTGGACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTc                                    <  1:5180623/93‑1 (MQ=14)
                                                           aaaaaaaaaaaGAAAGAAAAGATAATGGGTGCGGG‑‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTc                                    <  2:2330749/92‑1 (MQ=14)
                                                            aaaCAAGAAAGGAAGTAAAGATAATTGGTGC‑GA‑‑‑G‑GGGGGGGGC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTa                                  <  1:6849400/93‑1 (MQ=255)
                                                             aaCAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTa                                  >  2:6385833/1‑93 (MQ=255)
                                                             aaCAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTa                                  >  2:8085050/1‑93 (MQ=255)
                                                             aaCAAGAAAGGAAGTAAAGATAATTGGTGC‑GA‑‑‑GTGGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTa                                  >  2:4456123/1‑93 (MQ=255)
                                                              aCAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTAc                                 >  1:2707470/1‑93 (MQ=255)
                                                                aaGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCa                               >  1:2785448/1‑93 (MQ=255)
                                                                aaGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCa                               >  1:4963302/1‑93 (MQ=255)
                                                                 aGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCaa                              >  2:5109150/1‑93 (MQ=255)
                                                                  gAAAGGAAGTAAAGATAATTGGTGC‑GAGG‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACTAAAACCTGAAGCTAGCGCGTCTACCaa                              >  2:6825171/1‑93 (MQ=17)
                                                                  aaaagaaagTAAAAATATTGGGGGC‑GAG‑‑G‑GGGGGGGAC‑TTGACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAAt                             <  1:1981126/92‑1 (MQ=11)
                                                                   aaaGGAAGTAAAGATAATTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAAtt                            >  1:430889/1‑93 (MQ=255)
                                                                    aaGGAAGTAAAGATAATTGGTGC‑GAG‑‑G‑GGGGGGGGC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTc                           >  1:5001341/1‑93 (MQ=255)
                                                                    aaGGAAGTAAAGATAATTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTc                           >  1:7175548/1‑93 (MQ=255)
                                                                    aaGGAAGAAAAGATAATTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTc                           <  1:120206/93‑1 (MQ=14)
                                                                     aggaggTAAAGATAATTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTcc                          <  2:1499388/93‑1 (MQ=255)
                                                                     aGGAAGTAAAGATAAGTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTcc                          <  1:8227117/93‑1 (MQ=255)
                                                                      ggAAGTAAAGATAATTGGTGC‑GAGGGG‑GGGGGGGAC‑TTGAACCCCCACGTCCATAAGTACACGAACACCGGAAGCTGGCCGCTCCACCAATTc                           >  2:8623599/1‑93 (MQ=9)
                                                                       gAAGTAAAGATAATTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                        >  1:4123921/1‑93 (MQ=255)
                                                                       gAAGTAAAGATAATTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                        >  1:1703461/1‑93 (MQ=255)
                                                                        aaGTAAAGATAATTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGcc                       <  1:2456166/93‑1 (MQ=255)
                                                                          gTAAAGATAATTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGATAGCTCGTCTACCAATTCCGCCAc                     >  2:3879919/1‑93 (MQ=14)
                                                                           tAAAGATAATTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAcc                    >  1:2341688/1‑93 (MQ=255)
                                                                            aaaGATAATTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCt                   <  2:7612258/93‑1 (MQ=255)
                                                                            aaaGATAATTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCt                   >  1:1558775/1‑93 (MQ=255)
                                                                            aaaGATAATTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCt                   >  1:7020760/1‑93 (MQ=255)
                                                                            aaaGATAATTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCt                   <  1:6125072/93‑1 (MQ=255)
                                                                            aaaGATAATTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCt                   >  1:5572839/1‑93 (MQ=255)
                                                                            aaaGATAATTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCt                   >  2:4551158/1‑93 (MQ=255)
                                                                             aaGATAATTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCtt                  >  2:5628643/1‑93 (MQ=255)
                                                                              tgATAATTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTc                 <  1:443373/92‑1 (MQ=255)
                                                                              aGATAATTGGTGC‑GAG‑‑G‑GGGGGGGGC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTc                 >  1:7608601/1‑93 (MQ=255)
                                                                              aGATAATTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTc                 <  2:8757187/93‑1 (MQ=255)
                                                                               gATAATTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCg                >  2:1087468/1‑93 (MQ=255)
                                                                               gATAATTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCg                >  2:1365964/1‑93 (MQ=255)
                                                                                aTAATTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGc               >  1:7642291/1‑93 (MQ=255)
                                                                                 tAATTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGca              >  2:7880439/1‑93 (MQ=16)
                                                                                  aaTTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCTCCACCTTCGcac             >  1:7808299/1‑93 (MQ=255)
                                                                                     tGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGt          >  1:2759667/1‑93 (MQ=255)
                                                                                     tGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGt          <  1:4604953/93‑1 (MQ=255)
                                                                                     tGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGt          <  2:5691059/93‑1 (MQ=255)
                                                                                     tGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGt          <  1:1082627/93‑1 (MQ=255)
                                                                                      ggTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTc         <  2:7402778/93‑1 (MQ=255)
                                                                                             a‑‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCATAc  >  2:4528960/1‑93 (MQ=255)
                                                                                              |                                                                                                
TTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GA‑‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTAC  >  NC_000913/4603563‑4603747

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GA‑‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGA  >  NC_000913/4603570‑4603756
                                                                                                |                                                                                             
TGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GA‑‑GGGGGGGG                                                                                               >  SRR4302541.740647/1‑94 (MQ=60)
TGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GA‑‑GGGGGGGG                                                                                               >  SRR4302541.5991706/1‑94 (MQ=60)
 GGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GA‑‑GGGGGGGGG                                                                                              >  SRR4302541.1099734/1‑94 (MQ=60)
   CATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GA‑‑GGGGGGGGggt                                                                                            >  SRR4302541.2483323/1‑91 (MQ=60)
    ATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGGGACT                                                                                            >  SRR4302541.489972/1‑94 (MQ=60)
    ATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGGGACT                                                                                            >  SRR4302541.2797912/1‑94 (MQ=60)
      CATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GA‑‑GGGGGGGGgatttg                                                                                         >  SRR4302541.2409877/1‑88 (MQ=60)
       ATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGGGACTTGA                                                                                         >  SRR4302541.3999488/1‑94 (MQ=60)
        TGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGGGACTTGAA                                                                                        >  SRR4302541.378245/1‑94 (MQ=60)
         GCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGGGACTTGAAC                                                                                       >  SRR4302541.4311778/1‑94 (MQ=60)
          CTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GA‑‑GGGGGGGGggggggttga                                                                                     >  SRR4302541.344569/1‑84 (MQ=60)
           TGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAGGGGGGGGGGACTTAAACC                                                                                      >  SRR4302541.6536584/1‑94 (MQ=60)
           TGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGGGACTTGAACCC                                                                                     >  SRR4302541.6359315/1‑94 (MQ=60)
            GATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGGGACTTGAACCCC                                                                                    <  SRR4302541.2373842/94‑1 (MQ=60)
              TGATNATGAATTAGAATCAGGAANAAAAANACAAGAAAGAAACAANAAAAGACGTAANGAAAATTGGTGC‑GA‑‑GGGGGGGGGCTTGAACCCCCAC                                                                                 <  SRR4302541.3741384/94‑1 (MQ=32)
                ATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAGGGGGGGGGGACTTGAACCCCCAC                                                                                 <  SRR4302541.1307888/94‑1 (MQ=60)
                ATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGGGACTTGAACCCCCACG                                                                                >  SRR4302541.6290150/1‑94 (MQ=60)
                ATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGGGACTTGAACCCCCACG                                                                                <  SRR4302541.4511969/94‑1 (MQ=60)
                ATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGGGACTTGAACACCCACG                                                                                >  SRR4302541.2160852/1‑94 (MQ=60)
                 AAATGAATTTGAAGCAGGGATGAAAAATAAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GA‑‑GGGGGGGGACTTGAACCCCCACGTC                                                                              <  SRR4302541.2782227/94‑1 (MQ=60)
                  AATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGGGACTTGAACCCCCACGTC                                                                              >  SRR4302541.5604808/1‑94 (MQ=60)
                  AATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGGGACTTGAACCCCCACGTC                                                                              >  SRR4302541.1983630/1‑94 (MQ=60)
                   ATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGGGACTTGAACCCCCACGTCC                                                                             >  SRR4302541.4928271/1‑94 (MQ=60)
                   ATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGGGACTTGAACCCCCACGTCC                                                                             <  SRR4302541.7134987/94‑1 (MQ=60)
                   ATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGGGACTTGAACCCCCACGTCC                                                                             <  SRR4302541.2276026/94‑1 (MQ=60)
                     GAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCCGT                                                                           >  SRR4302541.389789/1‑94 (MQ=60)
                     GAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGGGACTTGAACCCCCACGTCCGT                                                                           >  SRR4302541.4432884/1‑94 (MQ=60)
                     GAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGGGACTTGAACCCCCACGTCCGA                                                                           >  SRR4302541.5537847/1‑94 (MQ=60)
                     CAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATCATTGGCGC‑GAG‑GGGGGGGGACTTGAACCCACACGTCCGC                                                                           >  SRR4302541.4432883/1‑94 (MQ=60)
                       ATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAA                                                                         >  SRR4302541.2504019/1‑94 (MQ=60)
                          TGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GA‑‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGAC                                                                     <  SRR4302541.7097260/94‑1 (MQ=60)
                           aaatcaggaagaAAAAATAAAGGAAGAAAAAAGAAGGAAAGTAAAGATAATGGGTGC‑GAG‑GGGGGGGGACTTGACCCCCCACGTCCGTAAGGAC                                                                     <  SRR4302541.8280207/82‑1 (MQ=28)
                            AATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACA                                                                    >  SRR4302541.768221/1‑94 (MQ=60)
                            AATCAGGGATAAAAAATCAAGGAAAAAAAAAGAAAGGAAGTAAAGATAATGGGGGC‑GAGGGGGGGGGGGCTTGACCCCCCACGTCCGTAAGGAC                                                                     <  SRR4302541.4466270/94‑1 (MQ=44)
                              tcaggtagaAAAAATAAAGGAAGAAACAAGAAAGAAAGAAAAGATAGTTGGTGC‑GAG‑GGGGGGGGACTTGACCCCCCACGTCCGTAAGGACACT                                                                  <  SRR4302541.5456125/85‑1 (MQ=36)
                              TCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACT                                                                  <  SRR4302541.5849132/94‑1 (MQ=60)
                              ACAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GA‑‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTA                                                                 <  SRR4302541.8778279/94‑1 (MQ=60)
                               CAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTA                                                                 >  SRR4302541.6015455/1‑94 (MQ=60)
                               CAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTA                                                                 >  SRR4302541.7715663/1‑94 (MQ=60)
                               CAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTA                                                                 >  SRR4302541.5593972/1‑94 (MQ=60)
                                AGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGGACACTAA                                                                >  SRR4302541.6979008/1‑94 (MQ=60)
                                 GGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAAC                                                               <  SRR4302541.8708307/94‑1 (MQ=60)
                                   GATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAGGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACA                                                              >  SRR4302541.3908261/1‑94 (MQ=60)
                                   GATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGGGACTTGAACCCCCATGTCCGTAAGGACACTAACAC                                                             >  SRR4302541.1309125/1‑94 (MQ=60)
                                     TGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGGGACTTGACCCCCCACGTCCGTAAGGACACTAACACCT                                                           <  SRR4302541.7405046/94‑1 (MQ=60)
                                     TGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GA‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGGACACTAACACCTG                                                          >  SRR4302541.2331990/1‑94 (MQ=60)
                                       AAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGA                                                         >  SRR4302541.1493180/1‑94 (MQ=60)
                                       AAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GA‑‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAA                                                        >  SRR4302541.6080997/1‑94 (MQ=60)
                                        AAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAA                                                        >  SRR4302541.2810326/1‑94 (MQ=60)
                                         tacttttttttaattttttagttcattttttaaaggtggtggtgggggGGGGGGGGACTTGACCCCCCACGTCCGTAAGGACACTAACACCTGA                                                         <  SRR4302541.4742021/46‑1 (MQ=0)
                                          AATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAGTTGGTGC‑GAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGC                                                      >  SRR4302541.2500856/1‑94 (MQ=60)
                                           ATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGGGACTTGCACCCCCACGTCCGTAAGGACACTAACACCTGACGCT                                                     >  SRR4302541.7148915/1‑94 (MQ=60)
                                           ATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCG                                                     >  SRR4302541.830311/1‑94 (MQ=60)
                                            TCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTA                                                    >  SRR4302541.2865690/1‑94 (MQ=60)
                                            ACAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTA                                                    <  SRR4302541.5548807/94‑1 (MQ=60)
                                            ACAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTA                                                    <  SRR4302541.5714017/94‑1 (MQ=60)
                                              AAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGC                                                  <  SRR4302541.5403207/94‑1 (MQ=60)
                                              AAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGC                                                  >  SRR4302541.6450265/1‑94 (MQ=60)
                                              AAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGC                                                  >  SRR4302541.2264521/1‑94 (MQ=60)
                                              AAGGAAGAAACAAGAAAGGAAGTAAAGATAATGGGTGC‑GAG‑GGGGGGGGACTTGACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGC                                                  <  SRR4302541.8194245/94‑1 (MQ=60)
                                              AAGGAAGAAAAAAGAAAGGAAGTAAAGATAATTGGGGC‑GAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGC                                                  <  SRR4302541.3812030/94‑1 (MQ=52)
                                               AGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCG                                                 >  SRR4302541.4530355/1‑94 (MQ=60)
                                               AGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCG                                                 <  SRR4302541.4316132/94‑1 (MQ=60)
                                               AGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCG                                                 >  SRR4302541.6795361/1‑94 (MQ=60)
                                                GGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGC                                                >  SRR4302541.3347600/1‑94 (MQ=60)
                                                   AGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAGGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGT                                              >  SRR4302541.889494/1‑94 (MQ=60)
                                                   AGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAGGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGT                                              >  SRR4302541.889493/1‑94 (MQ=60)
                                                   AGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTC                                             >  SRR4302541.7253906/1‑94 (MQ=60)
                                                   AGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GA‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCT                                            >  SRR4302541.4759568/1‑94 (MQ=60)
                                                    GAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCT                                            >  SRR4302541.1959246/1‑94 (MQ=60)
                                                    GAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCT                                            >  SRR4302541.201094/1‑94 (MQ=60)
                                                    GAAACAAAAAAGGAATGAAAAATAATTGGTGCTGG‑‑GGGGGGGGACTTGGACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCT                                            <  SRR4302541.5233921/94‑1 (MQ=0)
                                                    AAAACAAGAAAGGAAGTAAAGATAATTGGGGC‑GAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCT                                            <  SRR4302541.5233920/94‑1 (MQ=49)
                                                     AAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTA                                           >  SRR4302541.8133477/1‑94 (MQ=59)
                                                     AAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTA                                           >  SRR4302541.8749060/1‑94 (MQ=59)
                                                     AAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GA‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTAC                                          <  SRR4302541.6922618/94‑1 (MQ=60)
                                                      AACAAGAATGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGGGCCTTAAACCCCACCGTCCGTAAGGAAACTAACACCgcaacttagcccctctac                                          >  SRR4302541.2734418/1‑76 (MQ=36)
                                                      AACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTAC                                          >  SRR4302541.2734419/1‑94 (MQ=56)
                                                      AACAAGAAAGGAAGTAAAGATAATTGGTGC‑GA‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAATGCGACTGACACCTGACGCTAGCGCGTCTACG                                         >  SRR4302541.6048780/1‑94 (MQ=46)
                                                       ACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACC                                         <  SRR4302541.2416951/94‑1 (MQ=54)
                                                        CAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCA                                        >  SRR4302541.2813543/1‑94 (MQ=51)
                                                        CAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCA                                        >  SRR4302541.6922601/1‑94 (MQ=51)
                                                        CAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCA                                        >  SRR4302541.5014120/1‑94 (MQ=51)
                                                         AAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTcccat                                       >  SRR4302541.2196983/1‑89 (MQ=50)
                                                         AAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAA                                       <  SRR4302541.2819958/94‑1 (MQ=49)
                                                           GAAAGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATT                                     <  SRR4302541.8796374/94‑1 (MQ=44)
                                                           GAAAGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATT                                     >  SRR4302541.5540536/1‑94 (MQ=44)
                                                           GAAAGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATT                                     >  SRR4302541.434944/1‑94 (MQ=44)
                                                           GAAAGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATT                                     <  SRR4302541.8369370/94‑1 (MQ=44)
                                                           AAAAGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATT                                     <  SRR4302541.7185791/94‑1 (MQ=41)
                                                            AAAGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTC                                    >  SRR4302541.5052726/1‑94 (MQ=40)
                                                            AAAGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTC                                    >  SRR4302541.168495/1‑94 (MQ=42)
                                                            AAAGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTC                                    >  SRR4302541.7252068/1‑94 (MQ=42)
                                                             AAGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCC                                   >  SRR4302541.6841972/1‑94 (MQ=39)
                                                             AAGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCC                                   <  SRR4302541.4405246/94‑1 (MQ=39)
                                                             AAGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCC                                   >  SRR4302541.2092141/1‑94 (MQ=39)
                                                             AAGGAAGAAAAGATAATTGGTGC‑GAG‑GGGGGGGGACTTGACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCC                                   <  SRR4302541.121166/94‑1 (MQ=25)
                                                              AGGAAGTAAAGATAATTGGTGC‑GA‑‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACAGTAACACCTGAAGCTAGCGCGTGTACCAATTCgta                                 >  SRR4302541.659601/1‑91 (MQ=37)
                                                              AGGAAGTAAAGATAAGTGGTGC‑GAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCG                                  <  SRR4302541.8316825/94‑1 (MQ=23)
                                                               GGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGC                                 >  SRR4302541.1720146/1‑94 (MQ=34)
                                                               GGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGC                                 >  SRR4302541.4164653/1‑94 (MQ=34)
                                                               GGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGC                                 >  SRR4302541.4164652/1‑94 (MQ=34)
                                                               GGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGCCTACCAATTCCGC                                 >  SRR4302541.2575115/1‑94 (MQ=33)
                                                                 AAGTAAAGATAATTGGTGC‑GAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCC                               >  SRR4302541.1214562/1‑94 (MQ=29)
                                                                 AAGTAAAGATAATTGGTGC‑GAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCA                               <  SRR4302541.2480629/94‑1 (MQ=29)
                                                                  AGTAAAGATAATTGGTGC‑GAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAC                              >  SRR4302541.5586854/1‑94 (MQ=27)
                                                                   GTAAAGATAATTGGTGC‑GAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACC                             >  SRR4302541.2364432/1‑94 (MQ=25)
                                                                   GTAAAGATAATTGGTGC‑GAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACC                             >  SRR4302541.1589355/1‑94 (MQ=25)
                                                                    agaaataataatgggggcgagGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAC                              <  SRR4302541.5345579/73‑1 (MQ=0)
                                                                    TAAAGATAATTGGTGC‑GAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCT                            >  SRR4302541.7095402/1‑94 (MQ=22)
                                                                    TAAAGATAATTGGTGC‑GAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCT                            >  SRR4302541.5629889/1‑94 (MQ=22)
                                                                    TAAAGATAATTGGTGC‑GAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCT                            >  SRR4302541.1574388/1‑94 (MQ=22)
                                                                     AAAGATAATTGGTGC‑GAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTT                           >  SRR4302541.5381805/1‑94 (MQ=20)
                                                                     AAAGATAATTGGTGC‑GAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTT                           <  SRR4302541.6187625/94‑1 (MQ=20)
                                                                     AAAGATAATTGGTGC‑GAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTT                           <  SRR4302541.8815082/94‑1 (MQ=20)
                                                                     AAAGATAATTGGTGC‑GAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTT                           >  SRR4302541.4713609/1‑94 (MQ=20)
                                                                     AAAGATAATTGGTGC‑GAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTT                           >  SRR4302541.3389903/1‑94 (MQ=20)
                                                                     AAAGATAATTGGGGC‑GAG‑GGGGGGGGACTTGACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTT                           <  SRR4302541.4869468/94‑1 (MQ=6)
                                                                      AAGATAATTGGTGCGGG‑‑GGGGGGGGACTTGACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTC                          <  SRR4302541.5018559/94‑1 (MQ=4)
                                                                      AAGATAATTGGTGC‑GAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTC                          >  SRR4302541.7689906/1‑94 (MQ=13)
                                                                      AAGATAATTGGTGC‑GAG‑GGGGGGGGACTTGACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTC                          <  SRR4302541.5558724/94‑1 (MQ=13)
                                                                      AAGATAATTGGTGC‑GAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTC                          <  SRR4302541.1532693/94‑1 (MQ=14)
                                                                      AAGATAATTGGTGC‑GAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGAGTCTACCAATTCCGCCACCTTC                          >  SRR4302541.426033/1‑94 (MQ=13)
                                                                      AAGATAATGGGTGC‑GAG‑GGGGGGGGACTTGACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTC                          <  SRR4302541.4818591/94‑1 (MQ=4)
                                                                       TGATAATTGGTGC‑GAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCG                         <  SRR4302541.447546/94‑1 (MQ=7)
                                                                       AGATAATTGGTGC‑GAG‑GGGGGGGGTCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGGCGGCCAATTCCGCCGCCTTCG                         >  SRR4302541.7794260/1‑94 (MQ=7)
                                                                       AGATAATTGGTGC‑GAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCG                         <  SRR4302541.5862334/94‑1 (MQ=10)
                                                                       AGATAATTGGGGC‑GAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTCCCAATTCCGCCACCTTCG                         >  SRR4302541.562265/1‑94 (MQ=2)
                                                                        GATAATTGGTGC‑GAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGC                        >  SRR4302541.6302852/1‑94 (MQ=7)
                                                                        GATAATTGGTGC‑GAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGC                        >  SRR4302541.4999532/1‑94 (MQ=7)
                                                                        GATAATTGGTGC‑GAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGC                        >  SRR4302541.7723760/1‑94 (MQ=7)
                                                                         ATAATTGGTGC‑GAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCC                       >  SRR4302541.5620308/1‑94 (MQ=5)
                                                                         ATAATTGGTGC‑GAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCA                       >  SRR4302541.8044770/1‑94 (MQ=5)
                                                                         ATAATTGGTGC‑GAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCA                       >  SRR4302541.7118713/1‑94 (MQ=5)
                                                                          TAATTGGTGC‑GAGGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCA                       >  SRR4302541.5155198/1‑94 (MQ=2)
                                                                          TAATTGGTGC‑GAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGGGTCTACCAATTCCGCCACCTTCGCAC                      >  SRR4302541.2925105/1‑94 (MQ=4)
                                                                          TAATTGGTGC‑GAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCTCCACCTTCGCAC                      >  SRR4302541.7892610/1‑94 (MQ=4)
                                                                          TAATTGGTGC‑GAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCAC                      >  SRR4302541.2925104/1‑94 (MQ=5)
                                                                          TAATTGGTGC‑GAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCAC                      >  SRR4302541.5672688/1‑94 (MQ=5)
                                                                          TAATTGGTGC‑GA‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACA                     <  SRR4302541.8697809/94‑1 (MQ=8)
                                                                           AATTGGTGC‑GAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACA                     >  SRR4302541.8702053/1‑94 (MQ=5)
                                                                           AATTGGTGC‑GAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACA                     >  SRR4302541.43479/1‑94 (MQ=5)
                                                                            AGTGGTGC‑GAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAG                    <  SRR4302541.7277846/94‑1 (MQ=0)
                                                                             TTGGTGC‑GAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGT                   >  SRR4302541.2787408/1‑94 (MQ=5)
                                                                             TTGGTGC‑GAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAAGGCACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCCCCTTCGCACAGT                   >  SRR4302541.4887108/1‑94 (MQ=4)
                                                                              atgggtgcgggGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAG                    <  SRR4302541.7277847/83‑1 (MQ=3)
                                                                              TGGTGC‑GAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTC                  <  SRR4302541.1092728/94‑1 (MQ=7)
                                                                              TGGTGC‑GAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTC                  <  SRR4302541.4651698/94‑1 (MQ=7)
                                                                              TGGTGC‑GAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTC                  >  SRR4302541.2742646/1‑94 (MQ=7)
                                                                                GTGC‑GAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCAT                >  SRR4302541.5510270/1‑94 (MQ=12)
                                                                                 TGC‑GAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGccacc               >  SRR4302541.7085660/1‑89 (MQ=3)
                                                                                 TGC‑GAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATC               >  SRR4302541.3956415/1‑94 (MQ=20)
                                                                                   ggcgagGGGGGGGGACTTGACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATC               <  SRR4302541.6187963/88‑1 (MQ=21)
                                                                                   ggagagGGGGGGGGACTTGCACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATC               <  SRR4302541.6187964/88‑1 (MQ=21)
                                                                                   C‑GAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTT             >  SRR4302541.7380371/1‑94 (MQ=25)
                                                                                      gagGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTA            >  SRR4302541.2734545/4‑94 (MQ=29)
                                                                                      gagGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTA            >  SRR4302541.5597357/4‑94 (MQ=29)
                                                                                      G‑‑GGGGGGGGACTTGAACCCACACGTCCGTAAGGACACTAACACCTAAAGCGAGCGCGTCTACCAATTCCGCCACCTgcaccttgtcacacatag          >  SRR4302541.1412152/1‑76 (MQ=0)
                                                                                      G‑‑GGGGGGGGACTTAAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACT          <  SRR4302541.6638677/94‑1 (MQ=32)
                                                                                       agGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTAC           >  SRR4302541.2143997/3‑94 (MQ=32)
                                                                                       agGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTAC           >  SRR4302541.1342090/3‑94 (MQ=32)
                                                                                         GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTT         >  SRR4302541.5909355/1‑94 (MQ=37)
                                                                                          GGGGGGGACTTGAGCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTT        <  SRR4302541.4573584/94‑1 (MQ=38)
                                                                                          GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTT        <  SRR4302541.2297929/94‑1 (MQ=39)
                                                                                           GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTT       >  SRR4302541.6017111/1‑94 (MQ=42)
                                                                                           GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTT       >  SRR4302541.704557/1‑94 (MQ=42)
                                                                                           GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTT       <  SRR4302541.4449529/94‑1 (MQ=42)
                                                                                           GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTAT       >  SRR4302541.704556/1‑94 (MQ=36)
                                                                                            GGGGGACTTGAACCCCCACTTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTT      <  SRR4302541.2960730/94‑1 (MQ=43)
                                                                                            GGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTT      >  SRR4302541.3715604/1‑94 (MQ=45)
                                                                                            GGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTT      >  SRR4302541.4082177/1‑94 (MQ=45)
                                                                                              GGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTT    <  SRR4302541.3690014/94‑1 (MQ=50)
                                                                                                GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGA  <  SRR4302541.8843704/94‑1 (MQ=55)
                                                                                                |                                                                                             
TGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GA‑‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGA  >  NC_000913/4603570‑4603756

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑