Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	884	257.2	12958910	96.9%	12557183	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,603,663	(G)_8→9	noncoding (72/88 nt)	leuV ←	tRNA‑Leu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,603,655	1	.	G	94.3%	558.5 / 25.4	176	noncoding (80/88 nt)	leuV	tRNA‑Leu
Reads supporting (aligned to +/- strand): ref base . (6/4); new base G (107/59); total (113/63)
Fisher's exact test for biased strand distribution p-value = 7.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.32e-01

Only 100 of 216 total aligned reads displayed.

TTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GA‑‑GGGGGGG‑G‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCAT  >  NC_000913/4603563‑4603742

ttATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑Ga                                                                                             >  1:4833347/1‑93 (MQ=255)

 tATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GA‑‑g                                                                                          >  2:1572501/1‑93 (MQ=255)

 tATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GA‑‑g                                                                                          >  2:1754543/1‑93 (MQ=255)

  aTTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GA‑‑gg                                                                                         <  2:6015955/93‑1 (MQ=255)

   ttGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GA‑‑ggg                                                                                        <  2:4977888/93‑1 (MQ=255)

   ttGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GA‑‑ggg                                                                                        <  1:5015292/93‑1 (MQ=255)

   ttGCTGGCATCATGCTGATGATAATGAATTAGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GA‑‑ggg                                                                                        <  2:6463631/93‑1 (MQ=255)

     gCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GA‑‑ggggg                                                                                      <  1:1341747/93‑1 (MQ=255)

      cTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GA‑‑gggggg                                                                                     >  1:63326/1‑93 (MQ=255)

        ggCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAAAAAGGAAGGAAAGATAATTGGTGC‑GA‑‑ggggggg‑g                                                                                  <  2:5750071/93‑1 (MQ=255)

           atcatGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GA‑‑ggggggg‑ggcc                                                                               >  2:2746247/1‑90 (MQ=255)

            tcatGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GA‑‑ggggggg‑ggact                                                                              >  2:723067/1‑89 (MQ=255)

            tcatGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GA‑‑ggggggg‑ggact                                                                              <  2:509558/93‑5 (MQ=255)

            tcatGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GA‑‑ggggggg‑ggact                                                                              <  2:3018883/93‑5 (MQ=255)

            tcatGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GA‑‑ggggggg‑ggact                                                                              >  2:2533563/1‑89 (MQ=255)

              atGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GA‑‑GGGGGGG‑G‑ACTTGa                                                                           >  1:4412201/1‑93 (MQ=255)

               tGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGG‑G‑ACTTGa                                                                           >  2:5306639/1‑93 (MQ=255)

                 cTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GA‑‑GGGGGGG‑G‑ACTTGAAcc                                                                        >  2:1511676/1‑93 (MQ=255)

                   gatgatAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAGAGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGG‑G‑ACTTGAAccc                                                                       >  2:4289401/1‑93 (MQ=255)

                   gatgatAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGG‑G‑ACTTGAAccc                                                                       >  1:4358114/1‑93 (MQ=255)

                   gatgatAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGG‑G‑ACTTGAAccc                                                                       >  1:2146086/1‑93 (MQ=255)

                     tgatAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGG‑G‑ACTTGAAccccc                                                                     >  1:2398528/1‑93 (MQ=255)

                      gatAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGG‑G‑ACTTGAACCCCCa                                                                    <  2:4309279/93‑1 (MQ=255)

                      gatAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGG‑G‑ACTTGAACCCCCa                                                                    <  2:758151/93‑1 (MQ=255)

                      gatAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGG‑G‑ACTTGAACCCCCa                                                                    >  2:5940899/1‑93 (MQ=255)

                      gatAATGAATGTGAATGAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGG‑G‑ACTTGAACCCCCa                                                                    <  2:1975164/93‑1 (MQ=39)

                       atAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGG‑G‑ACTTGAACCCCCAc                                                                   >  1:3434601/1‑93 (MQ=255)

                         aaTGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGG‑G‑ACTTGAACCCCCACGt                                                                 <  2:2649484/93‑1 (MQ=255)

                         aaTGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGG‑G‑ACTTGAACCCCCACGt                                                                 >  2:3962974/1‑93 (MQ=255)

                          aTGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGG‑G‑GCTTGAACCCCCACGTc                                                                >  1:5726839/1‑93 (MQ=255)

                          aTGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGG‑G‑ACTTGAACCCCCACGTc                                                                >  1:3631432/1‑93 (MQ=255)

                          aTGAATTTAAAACAGGAATAAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGAAAATTGGTGG‑GAG‑GGGGGGG‑G‑ACTTGAACCCCCACGTc                                                                <  2:1362212/93‑1 (MQ=37)

                           tGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGG‑G‑ACTTGAACCCCCACGTcc                                                               >  1:3318119/1‑93 (MQ=255)

                            gAATTTGAATCAGGGATAAAAAATCAAGGAAGAAAAAAGAAAGGAAGAAAAGATAATTGGTGC‑GAG‑GGGGGGG‑G‑ACTTGAACCCCCACGTCCg                                                              <  1:1521119/93‑1 (MQ=37)

                             aaTTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGG‑G‑ACTTGAACCCCCACGTCCga                                                             >  1:2720389/1‑92 (MQ=255)

                             aaTTTGAAAAAGGGATAAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGGGC‑GAG‑GGGGGGG‑G‑ACTTGAACCCCCACGTCCGt                                                             <  2:5821605/93‑1 (MQ=37)

                                  gAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGG‑G‑ACTTGAACCCCCACGTCCGTAAGGa                                                        >  2:3234025/1‑93 (MQ=255)

                                   aaTCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGG‑G‑ACTTGAACCCCCACGTCCGTAAGGac                                                       <  1:6373330/93‑1 (MQ=255)

                                    aTCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGG‑G‑ACTTGAACCCCCACGTCCGTAAGGaca                                                      >  1:58508/1‑93 (MQ=255)

                                    aTCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGG‑G‑ACTTGAACCCCCACGTCCGTAACGaaa                                                      >  1:1086173/1‑91 (MQ=37)

                                     tCAGGGATAAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGG‑G‑ACTTGAACCCCCACGTCCGTAAGGacac                                                     <  1:707747/93‑1 (MQ=255)

                                      aaGGGATGAAAAATAAAGGAAGAAACAAGAAAGGAAGTAAAGATAATGGGTGC‑GAG‑GGGGGGG‑G‑ACTTGAACCCCCACGTCCGTAAGGACACt                                                    <  1:1795442/92‑1 (MQ=25)

                                       aGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGG‑G‑ACTTGAACCCCCACGTCCGTAAGGACACTa                                                   <  2:764915/93‑1 (MQ=255)

                                       aGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGG‑G‑ACTTGAACCCCCACGTCCGTAAGGACACTa                                                   >  2:6342593/1‑93 (MQ=255)

                                        gagATAAAAAAAAAAGAAAAAAACAAAAAAGAAAGAAAAGAAAATTGGTGC‑GA‑‑GGGGGGG‑GTACTTGACCCCCCACGTCCGTAAGGACACTaa                                                  <  2:3615485/91‑1 (MQ=12)

                                          gATGAAAAAACAAAGAAAAAACAAAAAAAGAAAGAAAAGTAATTGGGGCGGG‑‑GGGGGGG‑G‑ACTTGAACCCCCACGTCCGTAAGGACACTAaca                                                <  2:2142628/93‑1 (MQ=9)

                                           aTGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGG‑G‑ACTTGAACCCCCACGTCCGTAAGGACACTAacac                                               >  1:288190/1‑93 (MQ=255)

                                            agAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGGGC‑GA‑‑GGGGGGGAG‑ATTTGACCCCCCACGTCCGTAAGGACACTAACAcc                                              <  1:2913678/92‑1 (MQ=25)

                                             gAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGG‑G‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCt                                             <  2:1807695/93‑1 (MQ=255)

                                             gAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGG‑G‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCt                                             >  1:4773536/1‑93 (MQ=255)

                                               aaaaTCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGG‑G‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGa                                           >  1:6259374/1‑93 (MQ=255)

                                               aaaaTCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGG‑G‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGa                                           >  1:2235758/1‑93 (MQ=255)

                                               aaaaTCAAGGAAGAAAAAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGG‑G‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGa                                           <  2:4826942/93‑1 (MQ=255)

                                                 aaTCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGG‑G‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAg                                         <  2:6273681/93‑1 (MQ=255)

                                                  aTCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGG‑G‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGc                                        >  1:389031/1‑93 (MQ=255)

                                                  aTCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGG‑G‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGc                                        >  2:2257866/1‑93 (MQ=255)

                                                  aTCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGG‑G‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGc                                        >  1:3018994/1‑93 (MQ=255)

                                                   tCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGG‑G‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCt                                       >  1:5704298/1‑93 (MQ=255)

                                                      aGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGG‑G‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAgc                                    >  1:1905289/1‑93 (MQ=255)

                                                        gaagaaACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGG‑G‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAgcgc                                  <  1:4302625/93‑1 (MQ=255)

                                                        gaagaaACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGG‑G‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAgcgc                                  <  2:696558/93‑1 (MQ=255)

                                                        gaagaaACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGG‑G‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAgcgc                                  <  1:5398441/93‑1 (MQ=255)

                                                           gaaACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGG‑G‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTc                               >  2:6412312/1‑93 (MQ=255)

                                                              aCAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGG‑G‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTAc                            >  1:5583648/1‑93 (MQ=255)

                                                               cAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGG‑G‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTAcc                           >  2:5482839/1‑93 (MQ=255)

                                                                 aGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGG‑G‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCaa                         >  2:902591/1‑93 (MQ=255)

                                                                 aGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGG‑G‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCaa                         >  2:4888963/1‑93 (MQ=255)

                                                                   aaaGGAAGTAAAGATATTGGGGGC‑GAG‑GGGGGGG‑G‑ACTTGACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAAtt                       <  2:3871146/93‑1 (MQ=14)

                                                                   aaaGGAAGTAAAGATAATTGGTGC‑GAGGGGGGGGG‑G‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAAt                        >  2:5398455/1‑93 (MQ=17)

                                                                    aaGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGG‑G‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTc                      <  2:4076412/93‑1 (MQ=255)

                                                                     aGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGG‑G‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTcc                     <  1:794915/93‑1 (MQ=255)

                                                                     aGGAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGG‑G‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTcc                     >  2:3212116/1‑93 (MQ=255)

                                                                      ggAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGG‑G‑TTTTGAACCCCCACGTCCGTAAGGACACTAACACTTCAAGCTAGCACTGCAACAAATTCCg                    >  2:2772244/1‑93 (MQ=17)

                                                                      ggAAGTAAAGATAATTGGTGC‑GAG‑GGGGGGG‑G‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCg                    >  2:5802632/1‑93 (MQ=255)

                                                                        aaGTAAAGAAAGTGGGTGC‑GAG‑GGGGGGG‑G‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGcc                  <  2:238830/93‑1 (MQ=11)

                                                                         aGTAAAGATATTTGGGGC‑GA‑‑GGGGGGG‑GAACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCa                 <  2:380502/93‑1 (MQ=14)

                                                                         aGTAAAGATAATTGGTGC‑GAG‑GGGGGGG‑G‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCa                 >  1:3614514/1‑93 (MQ=255)

                                                                         aGTAAAGATAATTGGTGC‑GAG‑GGGGGGG‑G‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCa                 >  1:377582/1‑93 (MQ=255)

                                                                         aGTAAAGATAATTGGTGC‑GA‑‑GGGGGGG‑G‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAc                >  1:3232352/1‑93 (MQ=255)

                                                                          gTAAAGATAATTGGTGC‑GAG‑GGGGGGG‑G‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAc                >  1:1249089/1‑93 (MQ=255)

                                                                          atAAAGATAATTGGTGC‑GAG‑GGGGGGG‑G‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAc                <  2:5178218/92‑1 (MQ=255)

                                                                            aaaGATAATTGGTGC‑GAG‑GGGGGGG‑G‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCt              <  1:858802/93‑1 (MQ=255)

                                                                            aaaGATAATTGGTGC‑GAG‑GGGGGGG‑G‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCt              <  2:5896209/93‑1 (MQ=255)

                                                                             aggATAATTGGTGC‑GAG‑GGGGGGG‑G‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCtt             <  2:6368625/91‑1 (MQ=255)

                                                                             aaaaTAATTGGGGC‑GAG‑GGGGGGG‑G‑ACTTGACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCtt             <  2:3680573/90‑1 (MQ=11)

                                                                             aaGATAATTGGTGC‑GAG‑GGGGGGG‑G‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCtt             <  1:2014069/93‑1 (MQ=255)

                                                                             aaGATAATTGGTGC‑GAG‑GGGGGGG‑G‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCtt             >  2:2576457/1‑93 (MQ=255)

                                                                             aaGATAATTGGTGC‑GAG‑GGGGGGG‑G‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCtt             >  2:3288462/1‑93 (MQ=255)

                                                                             aaGATAATTGGTGC‑GAG‑GGGGGGG‑G‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCtt             <  1:1662303/93‑1 (MQ=255)

                                                                             aaGATAATTGGTGC‑GA‑‑GGGGGGG‑G‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTc            >  1:6398355/1‑93 (MQ=38)

                                                                              aGATAATTGGTGC‑GAG‑GGGGGGG‑G‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTc            >  2:1191502/1‑93 (MQ=255)

                                                                              aGATAATGGGTGC‑GAG‑GGGGGGG‑G‑ACTTGACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGCCTACCAATTCCGCCACCTTc            <  1:3652765/93‑1 (MQ=11)

                                                                              aGATAAGTGGTGC‑GAG‑GGGGGGG‑G‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTc            <  2:2009735/93‑1 (MQ=255)

                                                                               gATAATTGGTGC‑GAG‑GGGGGGG‑G‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGCTTACCAATTCCGCAACCttgc           >  1:5300364/1‑91 (MQ=11)

                                                                                  aaTTGGTGC‑GAG‑GGGGGGG‑G‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcac        >  2:2169227/1‑93 (MQ=16)

                                                                                   aTTGGTGC‑GAG‑GGGGGGG‑G‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcaca       >  1:1013259/1‑93 (MQ=16)

                                                                                    ttGGTGC‑GAG‑GGGGGGG‑G‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcacac      >  1:5265141/1‑92 (MQ=12)

                                                                                    ttGGTGC‑GAG‑GGGGGGG‑G‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAg      <  1:5341970/93‑1 (MQ=16)

                                                                                     tgttGC‑GAG‑GGGGGGG‑G‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGt     <  2:6358257/90‑1 (MQ=255)

                                                                                        tGC‑GAG‑GGGGGGG‑G‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCAt  >  2:1865032/1‑93 (MQ=255)

TTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GA‑‑GGGGGGG‑G‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCAT  >  NC_000913/4603563‑4603742

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GA‑GGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGA  >  NC_000913/4603576‑4603756

caggataataataataattttgaagcaggtataAAAAATCAAGAAAAAAACAAGAAAGGAAGAAAAGATAATTGGTGC‑GAGGGGGGGG‑GACTTG                                                                                          <  SRR4302534.1521490/61‑1 (MQ=51)

CATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GA‑GGGGGGG‑GACTTGA                                                                                         >  SRR4302534.4531296/1‑94 (MQ=60)

    CTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAGGGGGGGG‑GACTTGAACC                                                                                      <  SRR4302534.2260574/94‑1 (MQ=60)

    CTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAGGGGGGGG‑GACTTGAACC                                                                                      >  SRR4302534.2178124/1‑94 (MQ=60)

     TGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAGGGGGGGG‑GACTTGAACCC                                                                                     >  SRR4302534.4475703/1‑94 (MQ=60)

     TGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAGGGGGGGG‑GACTTGAACCC                                                                                     >  SRR4302534.2202720/1‑94 (MQ=60)

     TGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAGGGGGGGG‑GACTTGAACCC                                                                                     >  SRR4302534.2006899/1‑94 (MQ=60)

      GATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAGGGGGGGG‑GACTTGAACCCC                                                                                    >  SRR4302534.6550554/1‑94 (MQ=60)

       ATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAGGGGGGGG‑GACTTGAACCCCC                                                                                   >  SRR4302534.2462258/1‑94 (MQ=60)

       ATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAGGGGGGGG‑GACTTGAACCCCC                                                                                   >  SRR4302534.3732601/1‑94 (MQ=60)

       ATGATAATAAATTTGAATCAGGGATGAAAAATCAAGGAAGAGACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAGGGGGGGG‑GACTTGAACCCCC                                                                                   <  SRR4302534.6538906/94‑1 (MQ=60)

        TGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAGGGGGGGG‑GACTTGAACCCCCA                                                                                  <  SRR4302534.4938746/94‑1 (MQ=60)

         GATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAGGGGGGGG‑GACTTGAACCCCCAC                                                                                 >  SRR4302534.3526890/1‑94 (MQ=60)

          ATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAGGGGGGGG‑GACTTGAACCCCCACG                                                                                >  SRR4302534.3837848/1‑94 (MQ=60)

           TAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAGGGGGGGG‑GACTTGAACCCCCACGT                                                                               >  SRR4302534.5862968/1‑94 (MQ=60)

           TAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAGGGGGGGG‑GACTTGAACCCCCACGT                                                                               >  SRR4302534.5653304/1‑94 (MQ=60)

           AAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAGGGGGGGG‑GACTTGAACCCCCACGT                                                                               <  SRR4302534.2838997/94‑1 (MQ=60)

            AATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAGGGGGGGG‑GGCTTGAACCCCCACGTC                                                                              >  SRR4302534.5882009/1‑94 (MQ=60)

            AATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAGGGGGGGG‑GACTTGAACCCCCACGTC                                                                              >  SRR4302534.3729039/1‑94 (MQ=60)

            AATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAGGGGGGGG‑GACTTGAACCCCCACGTC                                                                              >  SRR4302534.5468965/1‑94 (MQ=60)

            AATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAGGGGGGGG‑GACTTGAACCCCCACGTC                                                                              >  SRR4302534.4980391/1‑94 (MQ=60)

             ATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAGGGGGGGG‑GACTTGAACCCCCACGTCC                                                                             >  SRR4302534.3406826/1‑94 (MQ=60)

               GAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAGGGGGGGG‑GACTTGAACCCCCACGTCCGT                                                                           >  SRR4302534.6100229/1‑94 (MQ=60)

               GAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAGGGGGGGG‑GACTTGAACCCCCACGTCCGA                                                                           >  SRR4302534.2792176/1‑94 (MQ=60)

               GAATTTGAATCAGGGATAAAAAATCAAGGAAGAAAAAAGAAAGGAAGAAAAGATAATTGGTGC‑GAGGGGGGGG‑GACTTGAACCCCCACGTCCGT                                                                           <  SRR4302534.1561061/94‑1 (MQ=60)

                   TTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAGGGGGGGG‑GACTTGAACCCCCACGTCCGTAAGG                                                                       <  SRR4302534.1916413/94‑1 (MQ=60)

                    TGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAGGGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGA                                                                      >  SRR4302534.2982356/1‑94 (MQ=60)

                    TGAATAAGGGATGAAAAATCAAGAAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAGGGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGA                                                                      <  SRR4302534.761610/94‑1 (MQ=60)

                      AATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAGGGGGGGG‑GACTTGAACCCCCACGTCCGTAAcgaaa                                                                    >  SRR4302534.1114420/1‑89 (MQ=60)

                      AATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAGGGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACA                                                                    >  SRR4302534.4458402/1‑94 (MQ=60)

                      AATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAGGGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACA                                                                    <  SRR4302534.854030/94‑1 (MQ=60)

                      AATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAGGGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACA                                                                    >  SRR4302534.60088/1‑94 (MQ=60)

                      AATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAGGGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACA                                                                    <  SRR4302534.6545414/94‑1 (MQ=60)

                       acacgggaaaaaaaaaacaagaaagaaaaaaaaaggaaagaaaagatattgggggcgggGGGGGGG‑GACTTGACCCCCCACGTCCGTAAGGACA                                                                    <  SRR4302534.4833984/35‑1 (MQ=0)

                        TCAGGGATAAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAGGGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACACT                                                                  <  SRR4302534.725127/94‑1 (MQ=60)

                         CAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAGGGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACACTA                                                                 >  SRR4302534.3375321/1‑94 (MQ=60)

                         AAGGGATGAAAAATAAAGGAAGAAACAAGAAAGGAAGTAAAGATAATGGGTGC‑GAGGGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACACTA                                                                 <  SRR4302534.1842078/94‑1 (MQ=60)

                           GGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAGGGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACACTAAC                                                               >  SRR4302534.4777808/1‑94 (MQ=60)

                            GGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAGGGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACA                                                              >  SRR4302534.1144911/1‑94 (MQ=60)

                             GATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAGGGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACAC                                                             >  SRR4302534.3152675/1‑94 (MQ=60)

                             GATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAGGGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACAC                                                             >  SRR4302534.295641/1‑94 (MQ=60)

                               TGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAGGGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCT                                                           >  SRR4302534.4903146/1‑94 (MQ=60)

                               AGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGGGC‑GA‑GGGGGGGAGATTTGACCCCCCACGTCCGTAAGGACACTAACACCT                                                           <  SRR4302534.2990151/94‑1 (MQ=60)

                                GAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAGGGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTG                                                          >  SRR4302534.5475394/1‑94 (MQ=60)

                                GAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAGGGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTG                                                          >  SRR4302534.362222/1‑94 (MQ=60)

                                AAAAAATAAAGAAAGAAAAAAGAAAGGAAGAAAAGATAATTGGTGC‑GAGGGGGGGG‑GACTTGACCCCCCACGTCCGTAAGGACACTAACACCTG                                                          <  SRR4302534.5926678/94‑1 (MQ=35)

                                 AAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAGGGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGA                                                         >  SRR4302534.4443013/1‑94 (MQ=60)

                                 AAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAGGGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGA                                                         >  SRR4302534.2295001/1‑94 (MQ=60)

                                 AAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAGGGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGA                                                         >  SRR4302534.6428966/1‑94 (MQ=60)

                                    TATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAGGGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGC                                                      >  SRR4302534.398975/1‑94 (MQ=60)

                                    AATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAGGGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGC                                                      >  SRR4302534.3098042/1‑94 (MQ=60)

                                    AATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GA‑GGGGGGG‑Gggctttgaaccccccggcccgtagggccccaaacacctgaac                                                     >  SRR4302534.5363270/1‑52 (MQ=60)

                                     ATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAGGGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCT                                                     >  SRR4302534.5858817/1‑94 (MQ=60)

                                      TCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAGGGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTA                                                    >  SRR4302534.4481717/1‑94 (MQ=60)

                                       CAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAGGGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAG                                                   >  SRR4302534.1936248/1‑94 (MQ=60)

                                        AAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAGGGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGC                                                  >  SRR4302534.1954989/1‑94 (MQ=60)

                                          gaaaaaaaaAAGAAAGAAAGAAAAGATAATTGGTGC‑GAGGGGGGGG‑GACTTGACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGC                                                <  SRR4302534.2600780/85‑1 (MQ=22)

                                          GGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAGGGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGC                                                >  SRR4302534.2630357/1‑94 (MQ=60)

                                           GAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAGGGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCG                                               <  SRR4302534.4419268/94‑1 (MQ=60)

                                           GAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAGGGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCG                                               <  SRR4302534.5543783/94‑1 (MQ=60)

                                           GAAAAAAAAAAAAAGGAAGAAAAGATATTTGGTGCGGG‑GGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCG                                               <  SRR4302534.1608949/94‑1 (MQ=3)

                                            AAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAGGGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGT                                              >  SRR4302534.3542455/1‑94 (MQ=60)

                                             AGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAGGGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTC                                             <  SRR4302534.5440182/94‑1 (MQ=60)

                                                AACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAGGGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTAC                                          >  SRR4302534.5734522/1‑94 (MQ=56)

                                                  CAAGAAAGGAAGTAAAGATAATTGGTGC‑GAGGGGGGGG‑GCCCTGAACCCCACCGTCCGAAAGGACCCTAAAACCTGAAGCTAGCGCCTCTTCCA                                        >  SRR4302534.2120867/1‑94 (MQ=40)

                                                  CAAGAAAGGAAGTAAAGATAATTGGTGC‑GAGGGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCA                                        >  SRR4302534.2382927/1‑94 (MQ=51)

                                                  AAAGAAAGGAAGTAAAGATAATGGGTGC‑GAGGGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACCCCTGAAGCTAGCGCGTCTACCA                                        <  SRR4302534.1764749/94‑1 (MQ=38)

                                                   AAGAAAGGAAGTAAAGATAATTGGTGC‑GAGGGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAA                                       >  SRR4302534.430040/1‑94 (MQ=49)

                                                    AGAAAGGAAGTAAAGATAATTGGTGC‑GAGGGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGAAACTAACACCTGAAGCTAGCGCGTCTACCAAT                                      >  SRR4302534.1292205/1‑94 (MQ=45)

                                                      AAAGGAAGTAAAGATAATTGGTGC‑GAGGGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTC                                    >  SRR4302534.853494/1‑94 (MQ=42)

                                                       AAGGAAGTAAAGATAATTGGTGC‑GAGGGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCC                                   >  SRR4302534.2896353/1‑94 (MQ=39)

                                                        aaataacaacggggttttggggcgggGGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCC                                   <  SRR4302534.4208249/68‑1 (MQ=0)

                                                        AGGAAGTAAAGATAATTGGTGC‑GAGGGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCG                                  <  SRR4302534.814857/94‑1 (MQ=37)

                                                        AGGAAGTAAAGATAATTGGTGC‑GAGGGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCG                                  >  SRR4302534.3047542/1‑94 (MQ=37)

                                                         GGAAGTAAAGATAATTGGTGC‑GAGGGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACtctttactc                                 >  SRR4302534.798154/1‑85 (MQ=36)

                                                         GGAAGAAAAGATAATTGGTGC‑GAGGGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGC                                 <  SRR4302534.5507448/94‑1 (MQ=21)

                                                           AAGTAAAGATAATTGGTGC‑GAGGGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCA                               >  SRR4302534.387231/1‑94 (MQ=29)

                                                           AAGTAAAGATAATTGGTGC‑GAGGGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCA                               >  SRR4302534.3711705/1‑94 (MQ=29)

                                                           AAGTAAAGATAATTGGTGC‑GA‑GGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAC                              >  SRR4302534.3318273/1‑94 (MQ=31)

                                                            AGTAAAGATAATTGGTGC‑GAGGGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAC                              >  SRR4302534.1282010/1‑94 (MQ=27)

                                                              TAAAGATAATTGGTGC‑GAGGGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCT                            >  SRR4302534.6588599/1‑94 (MQ=22)

                                                              TAAAGATAATTGGTGC‑GAGGGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCT                            <  SRR4302534.3991055/94‑1 (MQ=22)

                                                               AAAGATAATTGGTGC‑GAGGGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTT                           <  SRR4302534.880482/94‑1 (MQ=20)

                                                               AAAGATAATTGGTGC‑GA‑GGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTC                          >  SRR4302534.6571149/1‑94 (MQ=21)

                                                                AAGATAATTGGTGC‑GAGGGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTC                          <  SRR4302534.1705587/94‑1 (MQ=14)

                                                                AAGATAATTGGTGC‑GAGGGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTC                          <  SRR4302534.2066773/94‑1 (MQ=14)

                                                                 taaggtgggggtgcgagGGGGGGG‑GACTTGACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTC                          <  SRR4302534.2506110/77‑1 (MQ=0)

                                                                 aagaaaatgggggcgagGGGGGGG‑GACTTGACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTC                          <  SRR4302534.406632/77‑1 (MQ=0)

                                                                 AGATAATTGGTGC‑GAGGGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGCTTACCAATTCCGCAACCTTgc                         >  SRR4302534.5443391/1‑92 (MQ=8)

                                                                 AGATAATGGGTGC‑GAGGGGGGGG‑GACTTGACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGCCTACCAATTCCGCCACCTTCG                         <  SRR4302534.3750922/94‑1 (MQ=1)

                                                                   ATAATTGGTGC‑GAGGGGGGGG‑GGCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAAAGCGTCTACCAATTACGCCACCTTCGCC                       >  SRR4302534.461298/1‑94 (MQ=3)

                                                                    TAATTGGTGC‑GAGGGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCAC                      >  SRR4302534.3442316/1‑94 (MQ=5)

                                                                     AATTGGTGC‑GAGGGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACA                     >  SRR4302534.1039334/1‑94 (MQ=5)

                                                                      ATTGGTGC‑GAGGGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAC                    >  SRR4302534.5407380/1‑94 (MQ=2)

                                                                      ATTGGTGC‑GAGGGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAC                    >  SRR4302534.3951064/1‑94 (MQ=2)

                                                                       TTGGTGC‑GAGGGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGT                   <  SRR4302534.5485996/94‑1 (MQ=5)

                                                                        tgggtgcgggGGGGGGG‑GACTTGACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGT                   <  SRR4302534.3819539/84‑1 (MQ=6)

                                                                        TGGTGC‑GAGGGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTC                  >  SRR4302534.4137294/1‑94 (MQ=7)

                                                                           TGC‑GAGGGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATA               >  SRR4302534.4236546/1‑94 (MQ=12)

                                                                           TGC‑GAGGGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACACTCATA               >  SRR4302534.4229188/1‑94 (MQ=0)

                                                                            GC‑GAGGGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCT              >  SRR4302534.3074620/1‑94 (MQ=23)

                                                                            GC‑GAGGGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCT              >  SRR4302534.5986469/1‑94 (MQ=23)

                                                                             C‑GAGGGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTCATCTA             >  SRR4302534.6475461/1‑94 (MQ=6)

                                                                             C‑GAGGGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTT             <  SRR4302534.5192261/94‑1 (MQ=25)

                                                                               gggGGGGGGG‑GACTTGCACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTA            <  SRR4302534.3803296/91‑1 (MQ=28)

                                                                               gagGGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTA            >  SRR4302534.3935119/4‑94 (MQ=29)

                                                                               gagGGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTA            >  SRR4302534.689313/4‑94 (MQ=29)

                                                                               gagGGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCACCTTA            >  SRR4302534.2810255/4‑94 (MQ=10)

                                                                               GA‑GGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTAC           >  SRR4302534.2071824/1‑94 (MQ=31)

                                                                                G‑GGGGGGG‑GACTTGACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACT          <  SRR4302534.3646260/94‑1 (MQ=32)

                                                                                G‑GGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCAACCTTCGCACAGTaataatact          >  SRR4302534.3119021/1‑85 (MQ=5)

                                                                                   GGGGGG‑GACTTGACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTT        <  SRR4302534.4837450/94‑1 (MQ=38)

                                                                                   GGGGGG‑GACTTGACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTT        <  SRR4302534.1767967/94‑1 (MQ=38)

                                                                                   GGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCatctctcaatcggaagagcgt        >  SRR4302534.249180/1‑73 (MQ=0)

                                                                                   GGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTT        <  SRR4302534.5164716/94‑1 (MQ=39)

                                                                                   GGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCATACTTT        >  SRR4302534.4576595/1‑94 (MQ=25)

                                                                                     GGGG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTT      <  SRR4302534.5499823/94‑1 (MQ=45)

                                                                                     GGGG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTT      <  SRR4302534.5540620/94‑1 (MQ=45)

                                                                                     GGGG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTT      >  SRR4302534.829430/1‑94 (MQ=45)

                                                                                      GGG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTT     <  SRR4302534.3794985/94‑1 (MQ=47)

                                                                                        G‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTG   <  SRR4302534.4332886/94‑1 (MQ=52)

                                                                                        G‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTG   <  SRR4302534.816214/94‑1 (MQ=52)

                                                                                        G‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCATACTTTTTATG   >  SRR4302534.5993753/1‑94 (MQ=29)

                                                                                          GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGA  >  SRR4302534.4030875/1‑94 (MQ=55)

CATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GA‑GGGGGGG‑GACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGA  >  NC_000913/4603576‑4603756

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`