Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A0 F0 I1 R1 245 579.6 28867798 97.7% 28203838 96.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,194,577 2 bp→GC coding (97‑98/774 nt) yjaD → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,194,5770CG100.0% 1573.2 / NA 409R33A (CGG→GCG) yjaDhypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (187/222);  total (187/222)
*NC_0009134,194,5780GC99.8% 1544.9 / NA 409R33A (CGG→GCG) yjaDhypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base C (186/222);  minor base A (1/0);  total (187/222)
Fisher's exact test for biased strand distribution p-value = 4.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 435 total aligned reads displayed.

CGTATAATTGAAAAATTAGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAACGGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTACAACAGCAGCGGCGTC  >  NC_000913/4194487‑4194667
                                                                                          ||                                                                                         
cttataATTGAAAAATTAGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCa                                                                                       >  1:9320756/3‑96 (MQ=255)
cGTATAATTGAAAAATTAGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCa                                                                                       >  2:2630329/1‑96 (MQ=255)
  tataATTGAAAAATTAGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCaaa                                                                                     <  2:9507046/96‑1 (MQ=255)
  tataATTGAAAAATTAGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCaaa                                                                                     >  2:11563332/1‑96 (MQ=255)
  tataATTGAAAAATTAGATCACGGCTGGGGGGTCGGCAGCCATGGACAAAAATTATGGTTGCCGAAGGGAGAAGGGCCATATGGCGAAGCGGCaaa                                                                                     >  2:1644714/1‑96 (MQ=255)
   ataATTGAAAAATTAGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAAt                                                                                    <  2:6225843/96‑1 (MQ=255)
    taATTGAAAAATTAGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAAtt                                                                                   <  2:6019471/96‑1 (MQ=255)
    taATTGAAAAATTAGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAAtt                                                                                   <  2:5599542/96‑1 (MQ=255)
     aaTTGAAAAATTAGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATAATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGGGGCAAAttt                                                                                  >  1:6480930/1‑96 (MQ=255)
      aTTGAAAAATTAGATCCCGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTc                                                                                 <  1:3184752/96‑1 (MQ=255)
      aTTGAAAAATTAGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTc                                                                                 >  1:4608607/1‑96 (MQ=255)
       ttGAAAAATTAGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCg                                                                                >  2:13568630/1‑96 (MQ=255)
       ttGAAAAATTAGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCg                                                                                <  1:206171/96‑1 (MQ=255)
          aaaaaTTAGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATc                                                                             >  2:12295045/1‑96 (MQ=255)
           aaaaTTAGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCt                                                                            <  1:13649471/96‑1 (MQ=255)
            aaaTTAGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATcat                                                                           <  1:11469473/96‑3 (MQ=255)
            aaaTAAGAGCGCGGCTGGTGGCTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCtt                                                                           <  1:5280133/96‑1 (MQ=255)
              aTTAGATCACGGCTGGTGGGTCGTCAGCCATTAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTtgt                                                                         <  2:10353775/96‑1 (MQ=255)
              aTTAGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTtgt                                                                         <  1:4097286/96‑1 (MQ=255)
              aTTAGATCACGGCTGGGGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTtgt                                                                         >  2:523941/1‑96 (MQ=255)
               ttAGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTtgtg                                                                        <  1:147736/96‑1 (MQ=255)
               ttAGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTtgag                                                                        <  1:10838547/96‑3 (MQ=255)
                 aGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTggg                                                                      <  2:1953574/96‑1 (MQ=255)
                 aGATCACGGCTGGTGGGGCGTCAGCCATGAACAAAAATTATGGTTGCCGAAAGGAGAATTGCCATATTGCTAAGCGGCAAATTTCGATCGtgtgtg                                                                      >  1:4897232/1‑94 (MQ=255)
                     cACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAg                                                                  <  2:9295941/96‑1 (MQ=255)
                     cACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAg                                                                  <  1:11626378/96‑1 (MQ=255)
                     cACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAg                                                                  >  1:8027492/1‑96 (MQ=255)
                     cACGGCTGGGGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAg                                                                  >  1:10024850/1‑96 (MQ=255)
                     cACGGCTGGAGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAg                                                                  >  1:7037424/1‑96 (MQ=255)
                      aCGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAgc                                                                 <  2:6897694/96‑1 (MQ=255)
                      aCGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAgc                                                                 <  2:8420775/96‑1 (MQ=255)
                       cGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAgcg                                                                >  2:6299295/1‑96 (MQ=255)
                        ggCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAgcgc                                                               >  1:2797384/1‑96 (MQ=255)
                        ggCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAgcgc                                                               >  2:5442967/1‑96 (MQ=255)
                        ggCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAccgc                                                               <  2:12593636/96‑1 (MQ=255)
                          cTGGTGGGACGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAgcgcgc                                                             >  2:12961454/1‑96 (MQ=255)
                            ggtggGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCAc                                                           >  1:5595205/1‑96 (MQ=255)
                             gtggGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACt                                                          <  2:3926141/96‑1 (MQ=255)
                              tggGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTa                                                         <  2:6546927/96‑1 (MQ=255)
                                ggTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACa                                                       <  2:13755036/96‑1 (MQ=255)
                                ggTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACa                                                       >  1:8766230/1‑96 (MQ=255)
                                    gtcAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATc                                                   <  1:187663/96‑1 (MQ=255)
                                    gtcAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATc                                                   <  2:3381956/96‑1 (MQ=255)
                                    gtcAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATc                                                   >  2:1126318/1‑96 (MQ=255)
                                    ggcAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATc                                                   >  1:11733717/3‑96 (MQ=255)
                                     tcAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCg                                                  <  2:9733741/96‑1 (MQ=255)
                                     tcAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCg                                                  <  2:1492009/96‑1 (MQ=255)
                                     tcAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCg                                                  >  2:5033622/1‑96 (MQ=255)
                                     tcAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCg                                                  >  2:7885389/1‑96 (MQ=255)
                                     tcAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCg                                                  <  2:9054848/96‑1 (MQ=255)
                                     tcAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTAAAAATCg                                                  >  1:5981916/1‑96 (MQ=255)
                                     tcAGCCATGAACAAAAATTATGGCTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGTAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCg                                                  <  2:10153027/96‑1 (MQ=255)
                                      cAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATctg                                                 >  1:6283288/1‑94 (MQ=255)
                                       aGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGc                                                <  2:308171/96‑1 (MQ=255)
                                        gCCATGAACAAAAATTATGGTTGCCGAAGGGAGATTTGCCATATGGCGAAGCGGCAAATTTCGATCTTGGGGGTCGGCGCGCACTACAAATCGGCg                                               >  1:11914925/1‑96 (MQ=255)
                                         ccATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGa                                              >  2:6737373/1‑96 (MQ=255)
                                          cATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGaa                                             >  2:4536560/1‑96 (MQ=255)
                                           aTGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAAt                                            >  2:8017723/1‑96 (MQ=255)
                                           aTGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAAt                                            <  2:1819870/96‑1 (MQ=255)
                                           aTGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAAt                                            <  2:1239144/96‑1 (MQ=255)
                                            tGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGaacg                                           >  1:12884026/1‑94 (MQ=255)
                                            tGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATg                                           <  2:13392776/96‑1 (MQ=255)
                                            tGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATg                                           <  2:8135131/96‑1 (MQ=255)
                                             gAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAAggg                                          >  2:2389243/1‑96 (MQ=255)
                                             gAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATgg                                          >  2:11741979/1‑96 (MQ=255)
                                               aCAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCa                                        >  1:3604914/1‑96 (MQ=255)
                                                  aaaaTTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAggg                                     <  2:6095110/96‑1 (MQ=255)
                                                    aaTTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAggggg                                   <  1:14038256/96‑1 (MQ=255)
                                                    aaTTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAggggg                                   >  2:3226251/1‑96 (MQ=255)
                                                      ttATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGaa                                 <  1:11105559/96‑1 (MQ=255)
                                                       tATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAAc                                <  2:10605973/96‑1 (MQ=255)
                                                        aTGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAAcc                               >  1:2373582/1‑96 (MQ=255)
                                                         tGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCt                              >  2:4229995/1‑96 (MQ=255)
                                                           gTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGt                            <  1:12445084/96‑1 (MQ=255)
                                                             tgacGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGttt                          <  2:8876287/93‑1 (MQ=255)
                                                               ccGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGGACCTGTTTgg                        >  2:7730414/1‑96 (MQ=255)
                                                               ccGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTgg                        >  2:4748430/1‑96 (MQ=255)
                                                               ccGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTgg                        <  2:276730/96‑1 (MQ=255)
                                                                cGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTggg                       >  1:8327794/1‑95 (MQ=255)
                                                                cGAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGt                       >  2:4104267/1‑96 (MQ=255)
                                                                 gAAGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGGCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGGTTgggt                      >  2:7243098/1‑94 (MQ=255)
                                                                  aaGGGAGAATTGCCATATGGCGAAGCGGCAGATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTa                     <  2:14110981/96‑1 (MQ=255)
                                                                  aaGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGtca                     <  1:11054861/96‑3 (MQ=255)
                                                                   aGGGAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAg                    <  1:9670331/96‑1 (MQ=255)
                                                                    gggAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGGACCTGTTTGGTTAGt                   >  1:7309366/1‑96 (MQ=255)
                                                                    gggAGAATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGt                   >  2:2882950/1‑96 (MQ=255)
                                                                      gagaATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTac                 <  1:8165826/96‑1 (MQ=255)
                                                                      gagaATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTac                 >  2:13052962/1‑96 (MQ=255)
                                                                       agaATTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTaca                <  1:6789630/96‑1 (MQ=255)
                                                                          aTTGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACATGTTTGGTTAGTacaaca             <  1:10155734/96‑1 (MQ=255)
                                                                           ttGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTACAAcag            <  2:8731253/96‑1 (MQ=255)
                                                                            tGCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTACAAcagc           >  2:7382366/1‑96 (MQ=255)
                                                                             gCCATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTACAAcagga          <  1:7655904/96‑3 (MQ=255)
                                                                              ccATATGGCGCAGCGTCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTACAAcagcag         <  1:4388679/96‑1 (MQ=255)
                                                                              ccATATGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTACAAcagcag         >  2:8538693/1‑96 (MQ=255)
                                                                              caatatGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTACAAcagcag         >  1:10545884/3‑96 (MQ=255)
                                                                                 tatGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTACAACAGCAgcgg      <  1:12118317/96‑1 (MQ=255)
                                                                                   tGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTACAACAGCAgcggcg    <  1:8518767/96‑1 (MQ=255)
                                                                                    ggCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTACAACAGCAGCGGCGt   >  2:5342814/1‑96 (MQ=255)
                                                                                    ggCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTACAACAGCAGCGGCGt   >  1:13248466/1‑96 (MQ=255)
                                                                                          ||                                                                                         
CGTATAATTGAAAAATTAGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAACGGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTACAACAGCAGCGGCGTC  >  NC_000913/4194487‑4194667

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 215 total aligned reads displayed.
AGCTATGGATCGTATAATTGAAAAATTAGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑CGGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTACAACAGCAGCGGCGTCACGATATGG  >  NC_000913/4194477‑4194676
                                                                                                     |                                                                                                   
NNNTATGGATCGTATAATTGAAAAATTAGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑G                                                                                                     <  SRR4302518.13035708/101‑1 (MQ=60)
AGCTATGGATCGTATAATTGAAAAATTAGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑G                                                                                                     <  SRR4302518.1130247/101‑1 (MQ=60)
AGCTATGGATCGTATAATTGAAAAATTAGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑G                                                                                                     <  SRR4302518.5592737/101‑1 (MQ=60)
AGCTATGGATCGTATAATTGAAAAATTAGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑G                                                                                                     <  SRR4302518.2702857/101‑1 (MQ=60)
 GCTATGGATCGTATAATTGAAAAATTAGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAN‑NN                                                                                                    >  SRR4302518.14539413/1‑101 (MQ=60)
  CTATGGATCGTATAATTGAGAAATTAGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑NNN                                                                                                   >  SRR4302518.8846523/1‑101 (MQ=60)
   TATGGATCGTATAATTGAAAAATTAGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGGGAATTGCCATATGGCGAAGCGG                                                                                                   >  SRR4302518.4194592/1‑101 (MQ=60)
   TATGGATCGTATAATTGAAAAATTAGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGG                                                                                                   <  SRR4302518.6195937/101‑1 (MQ=60)
     TGGATCGTATAATTGAAAAATTAGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCA                                                                                                >  SRR4302518.1636869/1‑101 (MQ=60)
     NNNATCGTATAATTGAAAAATTAGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCA                                                                                                <  SRR4302518.13012855/101‑1 (MQ=60)
     NNNATCGTATAATTGAAAAATTAGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCA                                                                                                <  SRR4302518.12594440/101‑1 (MQ=60)
      GGATCGTATAGTTGAAAAATTAGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAA                                                                                               <  SRR4302518.451440/101‑1 (MQ=60)
      GGATCGTATAATTGAAAAATTAGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAA                                                                                               >  SRR4302518.6561630/1‑101 (MQ=60)
       NNNCGTATAATTGAAAAATTAGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAA                                                                                              <  SRR4302518.10358971/101‑1 (MQ=60)
       NNNCGTATAATTGAAAAATTAGATCACGCCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAA                                                                                              <  SRR4302518.10589752/101‑1 (MQ=60)
       GATCGTATAATTGAAAAATTAGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGCTGCCGAAGGGAGAACTGCCATATGGCGAA‑ACGGCAAA                                                                                              >  SRR4302518.5210134/1‑101 (MQ=60)
        NNNGTATAATTGAAAAATTAGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAAT                                                                                             <  SRR4302518.10123108/101‑1 (MQ=60)
         TCGTATAATTGAAAAATTAGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATT                                                                                            >  SRR4302518.1402969/1‑101 (MQ=60)
           NNNTAATTGAAAAATTAGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTC                                                                                          <  SRR4302518.11711177/101‑1 (MQ=60)
           GTATAATTGAAAAATTAGATCCCGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTC                                                                                          <  SRR4302518.3333132/101‑1 (MQ=60)
           GTATAATTGAAAAATTAGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTC                                                                                          <  SRR4302518.6643053/101‑1 (MQ=60)
            TATAATTGAAAAATTAGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCG                                                                                         <  SRR4302518.214473/101‑1 (MQ=60)
            NNNAATTGAAAAATTAGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCG                                                                                         <  SRR4302518.8809949/101‑1 (MQ=60)
            NNNAATTGAAAAATTAGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTCGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCG                                                                                         <  SRR4302518.12470136/101‑1 (MQ=60)
              TAATTGAAAAATTAGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCNNN                                                                                       >  SRR4302518.7589158/1‑101 (MQ=60)
              TAATTGAAAAATTAGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGAT                                                                                       <  SRR4302518.1408196/101‑1 (MQ=60)
              TAATTGAAAAATTAGATCACGGCTGGTGGGGCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCNNN                                                                                       >  SRR4302518.9306586/1‑101 (MQ=60)
               NNNTGAAAAATTAGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATC                                                                                      <  SRR4302518.11595813/101‑1 (MQ=60)
               NNNTGAAAAATTAGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATC                                                                                      <  SRR4302518.11456150/101‑1 (MQ=60)
               AATTGAAAAATTAGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATAATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GGGGCAAATTTCGATC                                                                                      >  SRR4302518.6812713/1‑101 (MQ=60)
                NNNGAAAAATTAGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCT                                                                                     <  SRR4302518.14275011/101‑1 (MQ=60)
                ATTGAAAAATTAGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTTCCGAAGGGAGAATTGCCATATGGGGAA‑GCGGCAAATTTCGANNN                                                                                     >  SRR4302518.11377133/1‑101 (MQ=60)
                ATTGAAAAATTAGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCT                                                                                     >  SRR4302518.4851162/1‑101 (MQ=60)
                 TGGAAAAATAAGAGCGCGGCTGGTGGCTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTT                                                                                    <  SRR4302518.5551358/101‑1 (MQ=60)
                 NNNAAAAATTAGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTT                                                                                    <  SRR4302518.9130553/101‑1 (MQ=60)
                 NNNAAAAATTAGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCAT                                                                                    <  SRR4302518.12006987/101‑1 (MQ=60)
                  TGAAAAATTAGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTTTGGTTGCCGAAGGGGGTGTAGCCATATGGCGAA‑GCGGCAAATTTCTATCNNN                                                                                   >  SRR4302518.12103118/1‑101 (MQ=60)
                  TGAAAAATTAGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTG                                                                                   <  SRR4302518.1192210/101‑1 (MQ=60)
                  TGAAAAATTAGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCNNN                                                                                   >  SRR4302518.13618411/1‑101 (MQ=60)
                   GAAAAATTAGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGT                                                                                  <  SRR4302518.404729/101‑1 (MQ=60)
                   GAAAAATTAGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGT                                                                                  <  SRR4302518.4295740/101‑1 (MQ=60)
                    NNNAATTAGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGAG                                                                                 <  SRR4302518.11347346/101‑1 (MQ=60)
                    NAAACTTAGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTG                                                                                 <  SRR4302518.153806/101‑1 (MQ=60)
                      AGATTAGATCACGGCTGGGGGGTCGTCAGCCATCAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTNNN                                                                               >  SRR4302518.7917968/1‑101 (MQ=60)
                      AAATTAGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGTAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGG                                                                               <  SRR4302518.2328693/101‑1 (MQ=60)
                      AAATTAGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTG‑GGGT                                                                              >  SRR4302518.3742878/1‑101 (MQ=60)
                       NNNTAGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGT                                                                              <  SRR4302518.12270698/101‑1 (MQ=60)
                       AATTAGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGNNN                                                                              >  SRR4302518.10340049/1‑101 (MQ=60)
                       AATTAGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGT                                                                              <  SRR4302518.6799494/101‑1 (MQ=60)
                        NNNAGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTTGATCTTGTGGGTC                                                                             <  SRR4302518.14483124/101‑1 (MQ=60)
                        NNNAGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTC                                                                             <  SRR4302518.8468239/101‑1 (MQ=60)
                        ATTAGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTC                                                                             <  SRR4302518.6904249/101‑1 (MQ=60)
                         TTAGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCTAA‑GAGGTAAATTTCGATCTTGTGGGNNN                                                                            >  SRR4302518.10794890/1‑101 (MQ=60)
                          NNNATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAG                                                                           <  SRR4302518.12173864/101‑1 (MQ=60)
                          GAGATCACGGCTGGTGGGGCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCGAAGTTCGATCTTGTGGGgnnn                                                                           >  SRR4302518.14497278/1‑97 (MQ=60)
                           AGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGC                                                                          <  SRR4302518.380272/101‑1 (MQ=60)
                           AGATCACGGCTGGTGGGGCGTCAGCCATGAACAAAAATTATGGTTGCCGAAAGGAGAATTGCCATATTGCTAA‑GCGGCAAATTTCGATCGTGTGTGTCCGC                                                                          >  SRR4302518.5152572/1‑101 (MQ=60)
                            GATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCANNN                                                                         >  SRR4302518.14614700/1‑101 (MQ=60)
                               CTCGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGC                                                                      <  SRR4302518.3914144/101‑1 (MQ=60)
                               CACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGNNN                                                                      >  SRR4302518.8427382/1‑101 (MQ=60)
                               CACGGCTGGTGGGGCGTCAGCCATGAACAAAAATTATGGTCGCCGAAGGGAGAATTGCCATATGGCTAA‑GCGGCAAATTTGGATCTTGTGGGCCAGCGNNN                                                                      >  SRR4302518.12095300/1‑101 (MQ=60)
                               CACGGCTGGGGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGNNN                                                                      >  SRR4302518.10500963/1‑101 (MQ=60)
                               CACGGCTGGAGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGNNN                                                                      >  SRR4302518.7410076/1‑101 (MQ=60)
                                ACGGCTGGGGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAGGGGGGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTCGGGGTCAGCGgccc                                                                     >  SRR4302518.5162386/1‑97 (MQ=60)
                                 CGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCAC                                                                    >  SRR4302518.561375/1‑101 (MQ=60)
                                  GGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACT                                                                   >  SRR4302518.2929936/1‑101 (MQ=60)
                                  GGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACT                                                                   >  SRR4302518.180433/1‑101 (MQ=60)
                                  GCCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCNNN                                                                   >  SRR4302518.12420752/1‑101 (MQ=60)
                                   GCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTA                                                                  <  SRR4302518.6620406/101‑1 (MQ=60)
                                    CTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTAC                                                                 <  SRR4302518.5036314/101‑1 (MQ=60)
                                     TGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGTA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTNNN                                                                >  SRR4302518.9030975/1‑101 (MQ=60)
                                     TGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTNNN                                                                >  SRR4302518.12200360/1‑101 (MQ=60)
                                     TGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACA                                                                <  SRR4302518.391754/101‑1 (MQ=60)
                                     CGGTGGGTCGTAACCCATGACCAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACA                                                                <  SRR4302518.4517876/101‑1 (MQ=60)
                                      GGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAA                                                               >  SRR4302518.5879000/1‑101 (MQ=60)
                                      GGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTGGTGGGTCAGCGCGCACTANNN                                                               >  SRR4302518.14852742/1‑101 (MQ=60)
                                        TGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAAT                                                             <  SRR4302518.2682334/101‑1 (MQ=60)
                                        TGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAAT                                                             <  SRR4302518.1502108/101‑1 (MQ=60)
                                         GGTTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATC                                                            <  SRR4302518.7065669/101‑1 (MQ=60)
                                         GGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATC                                                            <  SRR4302518.195259/101‑1 (MQ=60)
                                          nnntGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATAG                                                           <  SRR4302518.11178342/97‑1 (MQ=60)
                                          NNNCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCG                                                           <  SRR4302518.10251533/101‑1 (MQ=60)
                                          GGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGTCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCG                                                           >  SRR4302518.4992689/1‑101 (MQ=60)
                                          GGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCG                                                           <  SRR4302518.4295764/101‑1 (MQ=60)
                                          GGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAANNN                                                           >  SRR4302518.9192021/1‑101 (MQ=60)
                                           GTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATNNN                                                          >  SRR4302518.10715850/1‑101 (MQ=60)
                                            TCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTTCAAATCGGC                                                         <  SRR4302518.7324311/101‑1 (MQ=60)
                                            TAGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATAGGGCGAA‑GCGGGAATTTTCGATCTTGTGGGTCAGCGCGCACTACAAATCNNN                                                         >  SRR4302518.11169250/1‑101 (MQ=60)
                                            NNNTCAGCCATGAACAACAATTTTGGTTGCCGAAGGAAGAATTGCCAGATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTCCAAATCGtt                                                         <  SRR4302518.11836552/101‑3 (MQ=60)
                                             CGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGNNN                                                        >  SRR4302518.8576638/1‑101 (MQ=60)
                                              GGCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGNNN                                                       >  SRR4302518.12288821/1‑101 (MQ=60)
                                               TCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTAAAAATCGGCGAA                                                      >  SRR4302518.6285382/1‑101 (MQ=60)
                                                NNNCCATGAACAAAAATTATGGTTGCTGAAGGGAGAATTCCCATATGGCGAA‑GCGGCAAATTACAATCTTGTGGGTCAGCGCCCACTACAAATCGGTGAAT                                                     <  SRR4302518.10651618/101‑1 (MQ=60)
                                                NNNCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAAT                                                     <  SRR4302518.14253874/101‑1 (MQ=60)
                                                CAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCTGCGAAT                                                     >  SRR4302518.6603541/1‑101 (MQ=60)
                                                CAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAAT                                                     <  SRR4302518.5475422/101‑1 (MQ=60)
                                                  GGCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAANNN                                                   >  SRR4302518.11104251/1‑101 (MQ=60)
                                                  GCCATGAACAAAAATTATGGTTGCCGAAGGGAGATTTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGGGGGTCGGCGCGCACTACAAATCGGCGAtnnn                                                   >  SRR4302518.12482339/1‑97 (MQ=60)
                                                  GCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGG                                                   <  SRR4302518.2757619/101‑1 (MQ=60)
                                                   CCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATNNN                                                  >  SRR4302518.11585060/1‑101 (MQ=60)
                                                    CCTGAACAAAAATTATGGGTGCCGAAGGGAGAAATGCCATATGGGGAA‑GCGGCAAATTTCGATCTTGTGGGTCCGCGCGCACCACAAATaagagaatgnnn                                                 >  SRR4302518.8476983/1‑89 (MQ=60)
                                                    CATGAACAAAAATTATGGTTGCCGCAGGGTGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGcttaatcggcaacttcaaaacggcgaatgnnn                                                 >  SRR4302518.7682838/1‑69 (MQ=60)
                                                    CATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGNNN                                                 >  SRR4302518.8739402/1‑101 (MQ=60)
                                                    CATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACGACAAATCGGCGAATGGCA                                                 <  SRR4302518.5253911/101‑1 (MQ=60)
                                                    CATGAACAAAAATTATGGATGCCGGAGGGAGAAATGCCATATGGCGAA‑GCTGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGGATGGCA                                                 >  SRR4302518.1815358/1‑101 (MQ=60)
                                                      TGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAC‑GCGGCAAATTTCGACCTTGTTGGTCACGGCGCACTACAAATCGGCtgatgggnnn                                               >  SRR4302518.12099152/1‑91 (MQ=60)
                                                      TGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGG                                               <  SRR4302518.844940/101‑1 (MQ=60)
                                                      TGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGG                                               <  SRR4302518.2063588/101‑1 (MQ=60)
                                                      TGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAACGGCNNN                                               >  SRR4302518.13482311/1‑101 (MQ=60)
                                                      TGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGGGGGTCAGCGCGCACTACAAATCGGCGACTGGCAGG                                               >  SRR4302518.6870123/1‑101 (MQ=60)
                                                      TGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATAGGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCGGCGCGCACTACAAATCGGCGAATGGCNNN                                               >  SRR4302518.15038014/1‑101 (MQ=60)
                                                        NNNAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGG                                             <  SRR4302518.12889722/101‑1 (MQ=60)
                                                        AACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGNNN                                             >  SRR4302518.12136493/1‑101 (MQ=60)
                                                        AACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGG                                             >  SRR4302518.772126/1‑101 (MQ=60)
                                                         NNNAAAATTATGGTTGCCGAAGGGAGAATTGCCATGTGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGG                                            <  SRR4302518.13339259/101‑1 (MQ=60)
                                                         NNNAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGG                                            <  SRR4302518.8607323/101‑1 (MQ=60)
                                                         NNNAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGG                                            <  SRR4302518.14680837/101‑1 (MQ=60)
                                                         ACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGTGGG                                            >  SRR4302518.3773824/1‑101 (MQ=60)
                                                         ACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGG                                            <  SRR4302518.1125465/101‑1 (MQ=60)
                                                          CAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGA                                           >  SRR4302518.6407195/1‑101 (MQ=60)
                                                           NNNAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAA                                          <  SRR4302518.11625090/101‑1 (MQ=60)
                                                           AAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGGA                                          <  SRR4302518.3510483/101‑1 (MQ=60)
                                                           AAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAA                                          <  SRR4302518.7185894/101‑1 (MQ=60)
                                                            AAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAAC                                         <  SRR4302518.2800090/101‑1 (MQ=60)
                                                             NNNTTATGGTTGCCGAAGGGCAGATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACC                                        <  SRR4302518.13399291/101‑1 (MQ=60)
                                                             AAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGGACC                                        >  SRR4302518.5155665/1‑101 (MQ=60)
                                                             AAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCAATACAAATCGGCGAATGGCAGGGGGANNN                                        >  SRR4302518.10550451/1‑101 (MQ=60)
                                                              NNNTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGACAGCGCGCACTACAAATCGGTGAATGGCAGGGGGAACCT                                       <  SRR4302518.13218491/101‑1 (MQ=60)
                                                              AATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCT                                       <  SRR4302518.2153300/101‑1 (MQ=60)
                                                              AATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCG                                       >  SRR4302518.1698767/1‑101 (MQ=60)
                                                               NNNATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCCCTACAAATCGGCGAATGGCAGGGGGAACCTG                                      <  SRR4302518.14600138/101‑1 (MQ=60)
                                                               ATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACNNN                                      >  SRR4302518.9891307/1‑101 (MQ=60)
                                                               AGTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGGCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAccnnn                                      >  SRR4302518.12385833/1‑96 (MQ=60)
                                                                TTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTACGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCNNN                                     >  SRR4302518.12675947/1‑101 (MQ=60)
                                                                NNNTGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGT                                     <  SRR4302518.13030911/101‑1 (MQ=60)
                                                                 TATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGGACCTGTT                                    >  SRR4302518.5880339/1‑101 (MQ=60)
                                                                  NNNGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTT                                   <  SRR4302518.12781472/101‑1 (MQ=60)
                                                                  ATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTT                                   <  SRR4302518.4918991/101‑1 (MQ=60)
                                                                  ATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTT                                   >  SRR4302518.2488780/1‑101 (MQ=60)
                                                                  ATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTT                                   <  SRR4302518.6486535/101‑1 (MQ=60)
                                                                    NNNTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGACAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTCG                                 <  SRR4302518.10764443/101‑1 (MQ=60)
                                                                    GGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGGACTACAAATCGGCGAATGGCAGGGGGGACCTGTTNNN                                 >  SRR4302518.8133803/1‑101 (MQ=60)
                                                                     NNNGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGT                                <  SRR4302518.8706095/101‑1 (MQ=60)
                                                                     GTTGCCGAAGGGAGAATTGTTATATGGAGAT‑GCGGCAAATTTCGAGCTGGTGGGTCAGCGTGCACTACGAATCGGCGAATGGCAGGGGtgaactttttnnn                                >  SRR4302518.13992678/1‑88 (MQ=32)
                                                                     GTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTNNN                                >  SRR4302518.13613506/1‑101 (MQ=60)
                                                                      NNNCCGAAGGGAGAATTGCCATATGGCGCA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTT                               <  SRR4302518.10559098/101‑1 (MQ=60)
                                                                       NNNCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTCA                              <  SRR4302518.11572161/101‑1 (MQ=60)
                                                                        NNNGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAG                             <  SRR4302518.10136198/101‑1 (MQ=60)
                                                                        GCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAG                             <  SRR4302518.3126630/101‑1 (MQ=60)
                                                                        GCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGAAGGGGGAACCTGTGTGGTNNN                             >  SRR4302518.11357250/1‑101 (MQ=60)
                                                                         NNNAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGT                            <  SRR4302518.12107070/101‑1 (MQ=60)
                                                                         NNNAAGGGAAAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTCTGGTTAGT                            <  SRR4302518.13582055/101‑1 (MQ=60)
                                                                         CCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGT                            <  SRR4302518.4787612/101‑1 (MQ=60)
                                                                          CGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGgtannn                           >  SRR4302518.8737383/1‑95 (MQ=60)
                                                                          CGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTANNN                           >  SRR4302518.9165656/1‑101 (MQ=60)
                                                                          CGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTA                           >  SRR4302518.2112260/1‑101 (MQ=60)
                                                                           NNNGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTAC                          <  SRR4302518.8570175/101‑1 (MQ=60)
                                                                           NNNGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTAC                          <  SRR4302518.12600718/101‑1 (MQ=60)
                                                                           GGAGGGAGGATTGCCATATTGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCCCTACAAATGGGCGAATGGCATGGGGAACCCGTCTGGTTAGNNN                          >  SRR4302518.9430665/1‑101 (MQ=54)
                                                                            cacGGAGAAATGCCATATGTCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCCCTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTACA                         <  SRR4302518.3134522/98‑1 (MQ=60)
                                                                            AAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTACA                         <  SRR4302518.7146368/101‑1 (MQ=60)
                                                                             NNNGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTCTGGGTCAGCACGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTCCAA                        <  SRR4302518.7815876/101‑1 (MQ=60)
                                                                              GGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGGACCTGTTTGGTTAGTACNNN                       >  SRR4302518.7689888/1‑101 (MQ=60)
                                                                              GGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTACAAC                       <  SRR4302518.7026272/101‑1 (MQ=60)
                                                                              GGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGGTAGTACAAC                       >  SRR4302518.2673252/1‑101 (MQ=60)
                                                                               NNNGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACATGTTTGGTTAGTACAACA                      <  SRR4302518.10635861/101‑1 (MQ=60)
                                                                               GGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTACAACN                      >  SRR4302518.589421/1‑101 (MQ=60)
                                                                               GGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTACAACA                      <  SRR4302518.311694/101‑1 (MQ=60)
                                                                                 AGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTACAACNNN                    >  SRR4302518.9531013/1‑101 (MQ=60)
                                                                                  NNNTTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTACAACAGGA                   <  SRR4302518.8045623/101‑1 (MQ=60)
                                                                                  NNNTTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTACAACAGCA                   <  SRR4302518.7634750/101‑1 (MQ=60)
                                                                                  NNNTTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTACAACAGCA                   <  SRR4302518.8569813/101‑1 (MQ=60)
                                                                                  GAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTACAACAGCA                   <  SRR4302518.1996460/101‑1 (MQ=60)
                                                                                   NNNTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTACAACAGCCG                  <  SRR4302518.11641827/101‑1 (MQ=60)
                                                                                   AATTGCCATATGGCGCA‑GCGTCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTACAACAGCAG                  <  SRR4302518.4610156/101‑1 (MQ=60)
                                                                                   AATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTACAACAGCAG                  <  SRR4302518.1855107/101‑1 (MQ=60)
                                                                                    NNNGCCATATGGCGAA‑GCGGCATATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTACAACAGCAGC                 <  SRR4302518.8642705/101‑1 (MQ=60)
                                                                                    NNNGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTACAACAGCAGC                 <  SRR4302518.8262478/101‑1 (MQ=60)
                                                                                    ATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTACAACAGCAGC                 <  SRR4302518.4774625/101‑1 (MQ=60)
                                                                                     TTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCTAATGGCAGGGGGAACCTGTTTGGTTAGTACAACAGCAGCG                <  SRR4302518.222926/101‑1 (MQ=60)
                                                                                     TTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTACAACAGCAGCG                <  SRR4302518.5828173/101‑1 (MQ=60)
                                                                                      NNNCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTACAACAGCAGCGG               <  SRR4302518.12694272/101‑1 (MQ=60)
                                                                                       NNNATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTACAACAGCAGCGAC              <  SRR4302518.12297564/101‑1 (MQ=60)
                                                                                       GCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTACAACAGCAGCGGC              <  SRR4302518.909926/101‑1 (MQ=60)
                                                                                       GCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTACAACAGCAGCGGC              <  SRR4302518.5091348/101‑1 (MQ=60)
                                                                                       GCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTACAACAGCAGCGGC              <  SRR4302518.6196670/101‑1 (MQ=60)
                                                                                        NNNTATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTACAACAGCAGCGGCG             <  SRR4302518.8934575/101‑1 (MQ=60)
                                                                                         NNNATGGCGAA‑GCGGCAAATTTCGATCTAGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTACAACAGCAGCGGCGT            <  SRR4302518.13160783/101‑1 (MQ=60)
                                                                                          NNNTGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTACAACAGCAGCGGCGTC           <  SRR4302518.8006389/101‑1 (MQ=60)
                                                                                          NNNTGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTACAACAGCAGCGGCGTC           <  SRR4302518.7705688/101‑1 (MQ=60)
                                                                                          ATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTACAACAGCAGCGGCGTC           <  SRR4302518.6116215/101‑1 (MQ=60)
                                                                                           TATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTACAACAGCAGCGGCGTCA          <  SRR4302518.6211230/101‑1 (MQ=60)
                                                                                            ATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTACAACAGCAGCGGCGgnnn         >  SRR4302518.8393557/1‑97 (MQ=60)
                                                                                            ATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTACAACAGCAGCGGCGTCAC         <  SRR4302518.6222408/101‑1 (MQ=60)
                                                                                              GGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTACAACAGCAGCGGCGTCANNN       >  SRR4302518.13859208/1‑101 (MQ=60)
                                                                                               GCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTACAACAGCAGCGGCGTCACGAT      <  SRR4302518.3797394/101‑1 (MQ=60)
                                                                                                CGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTACAACAGCAGCGGCGTCACGATA     <  SRR4302518.1143056/101‑1 (MQ=60)
                                                                                                CGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTACAACAGCAGCGGCGTCACGATA     <  SRR4302518.2402705/101‑1 (MQ=60)
                                                                                                   N‑NNGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTACAACAGCAGCGGCGTCACGATATGG  <  SRR4302518.9207970/101‑1 (MQ=60)
                                                                                                   N‑NNGGCAAATTTCGATCTTGTGGGTCAGAGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTACAACAGCAGCGGCGTCACGATATGG  <  SRR4302518.12938619/101‑1 (MQ=60)
                                                                                                     |                                                                                                   
AGCTATGGATCGTATAATTGAAAAATTAGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑CGGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTACAACAGCAGCGGCGTCACGATATGG  >  NC_000913/4194477‑4194676

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑