Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 884 257.2 12958910 96.9% 12557183 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,237,790 T→C S69S (TCT→TCC)  ygjU → putative transport protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,237,7900TC99.6% 928.1 / ‑5.5 263S69S (TCT→TCC) ygjUputative transport protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (131/131);  minor base A (1/0);  total (132/131)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

Only 100 of 264 total aligned reads displayed.

GGCGGAAGCTGTTGGTCTGTTAGGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCTATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGTTCCTCTATCTACTGGGCACCTTCTCTGCTGCTCTGGCCGC  >  NC_000913/3237700‑3237879
                                                                                          |                                                                                         
ggcggAAGCTGTTGGTCTGTTAGGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCAt                                                                                         >  2:5798159/1‑93 (MQ=255)
ggcggAAGCTGTTGGCCTGTTAGGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCAt                                                                                         >  1:4391808/1‑93 (MQ=255)
 gcggAAGCTGTTGGTCTGTTAGGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCAtt                                                                                        <  2:4630339/93‑1 (MQ=255)
 gcggAAGCTGTTGGTCTGTTAGGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCAtt                                                                                        <  1:5951980/93‑1 (MQ=255)
   ggAAGCTGTTGGTCTGTTAGGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGc                                                                                      >  2:4689658/1‑93 (MQ=255)
    gAAGCTGTTGGTCTGTTAGGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCt                                                                                     >  1:648899/1‑93 (MQ=255)
       gCTGTTGGTCTGTTAGGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAac                                                                                  >  1:5107467/1‑93 (MQ=255)
       gCTGTTGGTCTGTTAGGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAac                                                                                  >  2:6158896/1‑93 (MQ=255)
         tGTTGGTCTGTTAGGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAacca                                                                                <  1:1508306/93‑1 (MQ=255)
          gTTGGTCTGTTAGGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAaccac                                                                               >  2:3309164/1‑93 (MQ=255)
           ttGGTCTGTTAGGTCCTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCTATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAaccacc                                                                              <  2:3114972/93‑1 (MQ=255)
           ttGGTCTGTTAGGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAaccacc                                                                              <  2:3975619/93‑1 (MQ=255)
            tGGTCTGTTAGGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAAccacca                                                                             >  2:3237558/1‑93 (MQ=255)
            tGGTCTGTTAGGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAAccacca                                                                             <  1:5454578/93‑1 (MQ=255)
             ggTCTGTTAGGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAg                                                                            >  1:3694555/1‑93 (MQ=255)
               tCTGTTAGGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCa                                                                          >  2:529079/1‑93 (MQ=255)
               tCTGTTAGGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCa                                                                          <  2:1817721/93‑1 (MQ=255)
                cTGTTAGGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCAc                                                                         <  1:468021/93‑1 (MQ=255)
                 tGTTAGGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACg                                                                        <  1:5194124/93‑1 (MQ=255)
                   ttAGGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACggg                                                                      <  2:251116/93‑1 (MQ=255)
                   ttAGGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACggg                                                                      <  2:770176/93‑1 (MQ=255)
                    tAGGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGc                                                                     <  1:3283844/93‑1 (MQ=255)
                     aGGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCa                                                                    <  1:4113281/93‑1 (MQ=255)
                     aGGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCa                                                                    <  1:2195678/93‑1 (MQ=255)
                      ggTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAg                                                                   <  2:4783893/93‑1 (MQ=255)
                      ggTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAg                                                                   >  2:649698/1‑93 (MQ=255)
                        tACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGaa                                                                 <  1:625901/93‑1 (MQ=255)
                        tACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGaa                                                                 <  2:41555/93‑1 (MQ=255)
                            ttGTTCGTCGGCGCACTGAAAGCCGTGGCGCCCATCCTGGTGTTGATGCTAGTGAGGGCATCCATTGCTAACCACCAGCACGGGCAGAAAAcc                                                             <  1:6191154/93‑1 (MQ=255)
                             tGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCa                                                            >  2:713545/1‑93 (MQ=255)
                              gTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCaa                                                           <  2:3490393/93‑1 (MQ=255)
                              gTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCaa                                                           >  2:6234580/1‑93 (MQ=255)
                              gTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCaa                                                           <  2:2614626/93‑1 (MQ=255)
                                tcgtcgGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAata                                                         <  2:753929/93‑1 (MQ=255)
                                  gtcgGCGCACTGAAGGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATc                                                       <  1:3846075/93‑1 (MQ=255)
                                  gtcgGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATc                                                       >  2:5859659/1‑93 (MQ=255)
                                  gtcgGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATc                                                       >  2:4332678/1‑93 (MQ=255)
                                  gtcgGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATc                                                       <  1:555279/93‑1 (MQ=255)
                                   tcgGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATcc                                                      >  2:4487022/1‑93 (MQ=255)
                                   tcgGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATcc                                                      <  1:3037108/93‑1 (MQ=255)
                                     ggCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGc                                                    >  1:2592856/1‑93 (MQ=255)
                                       cgcACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGccc                                                  >  2:3423401/1‑93 (MQ=255)
                                        gcACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCt                                                 >  2:5074051/1‑93 (MQ=255)
                                        gcACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCt                                                 >  1:2862864/1‑93 (MQ=255)
                                        gcACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCt                                                 >  1:1867847/1‑93 (MQ=255)
                                         cACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTa                                                >  2:1970088/1‑93 (MQ=255)
                                          aCTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTAt                                               <  2:3949762/93‑1 (MQ=255)
                                          aCTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTAt                                               <  1:4986286/93‑1 (MQ=255)
                                           cTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTAtt                                              <  1:1209986/93‑1 (MQ=255)
                                            tGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTAttt                                             <  1:703348/93‑1 (MQ=255)
                                            tGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTAttt                                             >  2:1673399/1‑93 (MQ=255)
                                            tGAAAGCAGTTGCCCCCATCCTGGTGTTGATTCTGGTGATAGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTAttt                                             <  1:852029/93‑1 (MQ=255)
                                             gAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTAtttt                                            >  1:1358170/1‑93 (MQ=255)
                                              aaaGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTg                                           <  2:38576/93‑1 (MQ=255)
                                              aaaGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTg                                           <  2:5556788/93‑1 (MQ=255)
                                               aatccGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGt                                          >  1:3551979/4‑93 (MQ=255)
                                               aaGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGt                                          <  2:5416061/93‑1 (MQ=255)
                                                aGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGtt                                         <  1:3993925/93‑1 (MQ=255)
                                                 gCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGTTc                                        <  2:3005927/93‑1 (MQ=255)
                                                  ccGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGTTcc                                       <  1:2719601/93‑1 (MQ=255)
                                                   cGTTGCCCCCATCCTGGTGTTGATGCTGTTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGTTCct                                      <  2:3496988/93‑1 (MQ=255)
                                                   cGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGGAAACCAATATCCGCCCTATTTTGTTCct                                      >  1:6031713/1‑93 (MQ=255)
                                                   cGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGTTCct                                      <  2:2186919/93‑1 (MQ=255)
                                                      tGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGTTCCtcta                                   <  1:1768183/93‑1 (MQ=255)
                                                        cccccATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGTTCCtctatc                                 <  2:5436179/93‑1 (MQ=255)
                                                         ccccATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGTTCCtctatct                                <  2:6307297/93‑1 (MQ=255)
                                                         ccccATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGTTCCtctatct                                <  1:3433402/93‑1 (MQ=255)
                                                         ccccATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGTTCCtctatct                                <  1:2478485/93‑1 (MQ=255)
                                                         ccccATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGCGCAGAAAACCAATATCCGCCCTATTTTGTTCCtctatct                                >  2:270964/1‑93 (MQ=255)
                                                          cccATCCTGTTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGTTCCtctatcta                               >  2:1045874/1‑93 (MQ=255)
                                                          cccATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGTTCCtctatcta                               <  2:127840/93‑1 (MQ=255)
                                                           ccATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGTTCCTCTATCTAc                              >  1:4561104/1‑93 (MQ=255)
                                                             aTCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGTTCCTCTATCTACTg                            <  1:2174503/93‑1 (MQ=255)
                                                              tCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGTTCCTCTATCTACTgg                           >  1:4544423/1‑93 (MQ=255)
                                                              tCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGTTCCTCTATCTACTgg                           <  1:5297005/93‑1 (MQ=255)
                                                                 tGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGTTCCTCTATCTACTGGGCa                        >  2:655200/1‑93 (MQ=255)
                                                                  ggTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGTTCCTCTATCTACTGGGCAc                       >  1:5649353/1‑93 (MQ=255)
                                                                   gtgtTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCAGCCCTATTTTGTTCCTCTATCTACTGGGCAcc                      >  2:1588802/1‑93 (MQ=255)
                                                                    tgtTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGTTCCTCTATCTACTGGGCACCt                     >  2:2797589/1‑93 (MQ=255)
                                                                    tgtTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGTTCCTCTATCTACTGGGCACCt                     <  1:5445407/93‑1 (MQ=255)
                                                                        gATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGTTCCTCTATCTACTGGGCACCTtctc                 <  1:6306875/93‑1 (MQ=255)
                                                                           tcTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGTTCCTCTATCTACTGGGCACCTTCTctgc              <  2:1376371/92‑1 (MQ=255)
                                                                           gCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGTTCCTCTATCTACTGGGCACCTTCTctgc              <  1:5336850/93‑1 (MQ=255)
                                                                            cTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGTTCCTCTATCTACTGGGCACCTTCTctgct             <  2:1075618/93‑1 (MQ=255)
                                                                              ggTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGTTCCTCTATCTACTGGGCACCTTCTCtgctgc           >  1:5765181/1‑93 (MQ=255)
                                                                               gTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGTTCCTCTATCTACTGGGCACCTTCTCtgctgcc          >  2:5908631/1‑92 (MQ=255)
                                                                                tGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGTTCCTCTATCTACTGGGCACCTTCTCTGCTGctc         <  1:4802035/93‑1 (MQ=255)
                                                                                 gATGGCATCCCTTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGTTCCTCTATCTACTGGGCACCTTCTCTGCTGctct        <  2:920423/93‑1 (MQ=255)
                                                                                 gATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGTTCCTCTATCTACTGGGCACCTTCTCTGCTGctct        >  1:2519053/1‑93 (MQ=255)
                                                                                  aTGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGTTCCTCTATCTACTGGGCACCTTCTCTGCTGCTCTg       <  2:3607221/93‑1 (MQ=255)
                                                                                   tGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGTTCCTCTATCTACTGGGCACCTTCTCTGCTGCTCTgg      >  1:525691/1‑93 (MQ=255)
                                                                                    ggCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGTTCCTCTATCTACTGGGCACCTTCTCTGCTGCTCTGGc     <  1:1178096/93‑1 (MQ=255)
                                                                                    ggCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGTTCCTCTATCTACTGGGCACCTTCTCTGCTGCTCTGGc     <  2:372403/93‑1 (MQ=255)
                                                                                    ggCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGTTCCTCTATCTACTGGGCACCTTCTCTGCTGCTCTGGc     >  1:2348744/1‑93 (MQ=255)
                                                                                    ggCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGTTCCTCTATCTACTGGGCACCTTCTCTGCTGCTCTGGc     >  2:3700358/1‑93 (MQ=255)
                                                                                    ggCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGTTCCTCTATCTACTGGGCACCTTCTCTGCTGCTCTGGc     >  1:1698102/1‑93 (MQ=255)
                                                                                     gCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGTTCCTCTATCTACTGGGCACCTTCTCTGCTGCTCTGGcc    >  2:2271505/1‑93 (MQ=255)
                                                                                     gCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGTTCCTCTATCTACTGGGCACCTTCTCTGCTGCTCTGGcc    <  1:3863725/93‑1 (MQ=255)
                                                                                      aaTCCATTGCTAACCACCAGCACGGGCAGAAAGCCAATATCCGCCCTATTTTGTTCCTCTATCTACTGGGCACCTTCTCTGCTGCTCTGGCCg   <  1:6232602/92‑1 (MQ=255)
                                                                                       aTCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGTTCCTCTATCTACTGGGCACCTTCTCTGCTGCTCTGGCCGc  >  1:3719475/1‑93 (MQ=255)
                                                                                          |                                                                                         
GGCGGAAGCTGTTGGTCTGTTAGGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCTATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGTTCCTCTATCTACTGGGCACCTTCTCTGCTGCTCTGGCCGC  >  NC_000913/3237700‑3237879

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGCGGCGGAAGCTGTTGGTCTGTTAGGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCTATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGTTCCTCTATCTACTGGGCACCTTCTCTGCTGCTCTGGCCGCAGTA  >  NC_000913/3237697‑3237883
                                                                                             |                                                                                             
CGCGGCGGAAGCTGTTGGTCTGTTAGGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCC                                                                                               <  SRR4302534.796853/94‑1 (MQ=60)
CGCGGCGGAAGCTGTTGGTCTGTTAGGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCC                                                                                               <  SRR4302534.2985840/94‑1 (MQ=60)
 GCGGCGGAAGCTGTTGGTCTGTTAGGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCA                                                                                              >  SRR4302534.1229192/1‑94 (MQ=60)
 GCGGCGGAAGCTGTTGGTCTGTTAGGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCA                                                                                              <  SRR4302534.3283109/94‑1 (MQ=60)
  CGGCGGAAGCTGTTGGTCTGTTAGGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCAT                                                                                             >  SRR4302534.2637075/1‑94 (MQ=60)
  CGGCGGAAGCTGTTGGCCTGTTAGGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCAT                                                                                             >  SRR4302534.4510254/1‑94 (MQ=60)
    GCGGAAGCTGTTGGTCTGTTAGGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTG                                                                                           <  SRR4302534.6113593/94‑1 (MQ=60)
    GCGGAAGCTGTTGGTCTGTTAGGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTG                                                                                           <  SRR4302534.4464601/94‑1 (MQ=60)
    GCGGAAGCTGTTGGTCTGTTAGGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTG                                                                                           <  SRR4302534.4058209/94‑1 (MQ=60)
     CGGAAGCTGTTGGTCTGTTAGGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGC                                                                                          >  SRR4302534.961155/1‑94 (MQ=60)
     CGGAAGCTGTTGGTCTGTTAGGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGC                                                                                          >  SRR4302534.5053315/1‑94 (MQ=60)
     CGGAAGCTGTTGGTCTGTTAGGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGC                                                                                          >  SRR4302534.2246998/1‑94 (MQ=60)
      GGAAGCTGTTGGTCTGTTAGGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCT                                                                                         <  SRR4302534.493197/94‑1 (MQ=60)
      GGAAGCTGTTGGTCTGTTAGGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCT                                                                                         >  SRR4302534.664635/1‑94 (MQ=60)
      GGAAGCTGTTGGTCTGTTAGGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCT                                                                                         <  SRR4302534.5524258/94‑1 (MQ=60)
      GGAAGCTGTTGGTCTGTTAGGGACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCT                                                                                         >  SRR4302534.1216270/1‑94 (MQ=60)
       GAAGCTGTTGGTCTGTTAGGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTA                                                                                        >  SRR4302534.178107/1‑94 (MQ=60)
        AAGCTGTTGGTCTGTTAGGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAA                                                                                       >  SRR4302534.39612/1‑94 (MQ=60)
        AAGCTGTTGGTCTGTTAGGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAA                                                                                       >  SRR4302534.2236481/1‑94 (MQ=60)
         AGCTGTTGGTCTGTTAGGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAAC                                                                                      >  SRR4302534.5245295/1‑94 (MQ=60)
          GCTGTTGGTCTGTTAGGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACC                                                                                     <  SRR4302534.5370647/94‑1 (MQ=60)
          GCTGTTGGTCTGTTAGGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACC                                                                                     >  SRR4302534.2536220/1‑94 (MQ=60)
            TGTTGGTCTGTTAGGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCAC                                                                                   <  SRR4302534.1547975/94‑1 (MQ=60)
             GTTGGTCTGTTAGGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACC                                                                                  <  SRR4302534.5446417/94‑1 (MQ=60)
               TGGTCTGTTAGGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAG                                                                                >  SRR4302534.3793892/1‑94 (MQ=60)
               TGGTCTGTTAGGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAG                                                                                <  SRR4302534.5601565/94‑1 (MQ=60)
               TGGTCTGTTAGGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAG                                                                                >  SRR4302534.6044129/1‑94 (MQ=60)
                   CTGTTAGGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACG                                                                            <  SRR4302534.3683638/94‑1 (MQ=60)
                   CTGTTAGGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACG                                                                            <  SRR4302534.479819/94‑1 (MQ=60)
                   CTGTTAGGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACG                                                                            >  SRR4302534.3398498/1‑94 (MQ=60)
                    TGTTAGGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGG                                                                           <  SRR4302534.5334183/94‑1 (MQ=60)
                     GTTAGGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGG                                                                          <  SRR4302534.3922486/94‑1 (MQ=60)
                      TTAGGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGC                                                                         >  SRR4302534.377705/1‑94 (MQ=60)
                      TTAGGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGC                                                                         <  SRR4302534.3600395/94‑1 (MQ=60)
                      TTAGGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGC                                                                         >  SRR4302534.466339/1‑94 (MQ=60)
                      TTAGGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGC                                                                         <  SRR4302534.5262345/94‑1 (MQ=60)
                       TAGGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCA                                                                        >  SRR4302534.4820689/1‑94 (MQ=60)
                       TAGGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCA                                                                        <  SRR4302534.3371373/94‑1 (MQ=60)
                        AGGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAG                                                                       <  SRR4302534.6633454/94‑1 (MQ=60)
                        AGGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAG                                                                       <  SRR4302534.2253768/94‑1 (MQ=60)
                        AGGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAG                                                                       >  SRR4302534.2549839/1‑94 (MQ=60)
                        AGGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAG                                                                       <  SRR4302534.4223933/94‑1 (MQ=60)
                         GGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGA                                                                      <  SRR4302534.3846387/94‑1 (MQ=60)
                         GGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGA                                                                      <  SRR4302534.2767803/94‑1 (MQ=60)
                          GTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAA                                                                     >  SRR4302534.622065/1‑94 (MQ=60)
                          GTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCACCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAA                                                                     >  SRR4302534.6426283/1‑94 (MQ=60)
                           TACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAA                                                                    <  SRR4302534.641085/94‑1 (MQ=60)
                           TACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAA                                                                    >  SRR4302534.318452/1‑94 (MQ=60)
                           TACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAA                                                                    >  SRR4302534.3272725/1‑94 (MQ=60)
                           TACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAA                                                                    <  SRR4302534.4750186/94‑1 (MQ=60)
                           TACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAA                                                                    >  SRR4302534.2146025/1‑94 (MQ=60)
                             CTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAAC                                                                  >  SRR4302534.926839/1‑94 (MQ=60)
                               TTGTTCGTCGGCGCACTGAAAGCCGTGGCGCCCATCCTGGTGTTGATGCTAGTGAGGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCA                                                                <  SRR4302534.6359623/94‑1 (MQ=60)
                                TGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAA                                                               >  SRR4302534.2739902/1‑94 (MQ=60)
                                TGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAA                                                               >  SRR4302534.3621332/1‑94 (MQ=60)
                                     GTCGGCGCACTGAAGGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCC                                                          <  SRR4302534.3949360/94‑1 (MQ=60)
                                     GTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCC                                                          <  SRR4302534.568965/94‑1 (MQ=60)
                                     GTCGGCGCACTG‑AAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCG                                                         <  SRR4302534.6203502/94‑1 (MQ=60)
                                      TCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCG                                                         <  SRR4302534.3966387/94‑1 (MQ=60)
                                      TCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCG                                                         >  SRR4302534.775413/1‑94 (MQ=60)
                                      TCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCG                                                         <  SRR4302534.2281319/94‑1 (MQ=60)
                                      TCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCG                                                         <  SRR4302534.3116745/94‑1 (MQ=60)
                                       CGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGC                                                        >  SRR4302534.2661485/1‑94 (MQ=60)
                                          CGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCT                                                     >  SRR4302534.5686346/1‑94 (MQ=60)
                                          CGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCT                                                     <  SRR4302534.5146920/94‑1 (MQ=60)
                                          CGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCT                                                     <  SRR4302534.1453638/94‑1 (MQ=60)
                                          CGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCT                                                     >  SRR4302534.1916452/1‑94 (MQ=60)
                                          CGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCT                                                     >  SRR4302534.2938055/1‑94 (MQ=60)
                                            CACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTAT                                                   >  SRR4302534.5431835/1‑94 (MQ=60)
                                             ACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATT                                                  >  SRR4302534.4463089/1‑94 (MQ=60)
                                             ACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATT                                                  <  SRR4302534.5121218/94‑1 (MQ=60)
                                              CTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTT                                                 <  SRR4302534.1241819/94‑1 (MQ=60)
                                              CTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTT                                                 <  SRR4302534.4811112/94‑1 (MQ=60)
                                               TGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTT                                                >  SRR4302534.1393960/1‑94 (MQ=60)
                                               TGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTT                                                <  SRR4302534.720597/94‑1 (MQ=60)
                                               TGAAAGCAGTTGCCCCCATCCTGGTGTTGATTCTGGTGATAGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTT                                                <  SRR4302534.873515/94‑1 (MQ=60)
                                                GAAAGCCGTTGACCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTG                                               >  SRR4302534.4119883/1‑94 (MQ=60)
                                                 AAATCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGT                                              >  SRR4302534.3647503/1‑94 (MQ=60)
                                                 AAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGT                                              >  SRR4302534.1915982/1‑94 (MQ=60)
                                                 AAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGT                                              >  SRR4302534.736971/1‑94 (MQ=60)
                                                   AGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGTTC                                            <  SRR4302534.4100838/94‑1 (MQ=60)
                                                    GCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGTTCC                                           <  SRR4302534.5468652/94‑1 (MQ=60)
                                                     CCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGGAAACCAATATCCGCCCTATTTTGTTCCT                                          >  SRR4302534.6195619/1‑94 (MQ=60)
                                                     CCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGTTCCT                                          <  SRR4302534.2791364/94‑1 (MQ=60)
                                                     CCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGTTCCT                                          >  SRR4302534.2864332/1‑94 (MQ=60)
                                                       GTTGCCCCCATCCTGGTGTTCATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGTTCCTCT                                        >  SRR4302534.6469298/1‑94 (MQ=60)
                                                         TGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGTTCCTCTAT                                      <  SRR4302534.1814106/94‑1 (MQ=60)
                                                           CCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGTTCCTCTATCT                                    <  SRR4302534.6394157/94‑1 (MQ=60)
                                                           CCCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGTTCCTCTATCT                                    <  SRR4302534.1781525/94‑1 (MQ=60)
                                                            CCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGTTCCTCTATCTA                                   <  SRR4302534.2544330/94‑1 (MQ=60)
                                                            CCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGTTCCTCTATCTA                                   >  SRR4302534.2052041/1‑94 (MQ=60)
                                                            CCCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGTTCCTCTATCTA                                   <  SRR4302534.3525649/94‑1 (MQ=60)
                                                             CCCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGTTCCTCTATCTAC                                  >  SRR4302534.4684765/1‑94 (MQ=60)
                                                              CCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGTTCCTCTATCTACT                                 <  SRR4302534.2951159/94‑1 (MQ=60)
                                                              CCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGTTCCTCTATCTACT                                 <  SRR4302534.5540059/94‑1 (MQ=60)
                                                              CCATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGTTCCTCTATCTACT                                 <  SRR4302534.1292040/94‑1 (MQ=60)
                                                                atagtgggcTTGATGCTGGTTATGCCATACATTGCTAACCAACAGCACGGGCAGAAAACCAATATCCGCCTTATTTTGTTCCTCTATCTTCTGG                               <  SRR4302534.6165062/85‑1 (MQ=60)
                                                                ATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGTTCCTCTATCTACTGG                               >  SRR4302534.4667621/1‑94 (MQ=60)
                                                                ATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGTTCCTCTATCTACTGG                               <  SRR4302534.2231949/94‑1 (MQ=60)
                                                                ATCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGTTACTCTATCTACTGG                               >  SRR4302534.6099742/1‑94 (MQ=60)
                                                                 TCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGTTCCTCTATCTACTGGG                              <  SRR4302534.5439966/94‑1 (MQ=60)
                                                                 TCCTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGTTCCTCTATCTACTGGG                              <  SRR4302534.1606073/94‑1 (MQ=60)
                                                                   CTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGTTCCTCTATCTACTGGGCA                            >  SRR4302534.2555054/1‑94 (MQ=60)
                                                                   CTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGTTCCTCTATCTACTGGGCA                            <  SRR4302534.3065354/94‑1 (MQ=60)
                                                                   CTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGTTCCTCTATCTACTGGGCA                            <  SRR4302534.687727/94‑1 (MQ=60)
                                                                   CTGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCACCCTATTTTGTTCCTCTATCTACTGGGCA                            >  SRR4302534.5598264/1‑94 (MQ=60)
                                                                    TGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGTTCCTCTATCTACTGGGCAC                           >  SRR4302534.5802277/1‑94 (MQ=60)
                                                                    TGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGTTCCTCTATCTACTGGGCAC                           <  SRR4302534.5356894/94‑1 (MQ=60)
                                                                    TGGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGTTCCTCTATCTACTGGGCAC                           >  SRR4302534.6381866/1‑94 (MQ=60)
                                                                     GGTGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGTTCCTCTATCTACTGGGCACC                          <  SRR4302534.1657771/94‑1 (MQ=60)
                                                                       TGTTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGTTCCTCTATCTACTGGGCACCTT                        <  SRR4302534.5592120/94‑1 (MQ=60)
                                                                         TTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGTTCCTCTATCTACTGGGCACCTTCT                      >  SRR4302534.5326282/1‑94 (MQ=60)
                                                                         TTGATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGTTCCTCTATCTACTGGGCACCTTCT                      <  SRR4302534.3795410/94‑1 (MQ=60)
                                                                           GATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGTTCCTCTATCTACTGGGCACCTTCTCT                    <  SRR4302534.6473388/94‑1 (MQ=60)
                                                                           GATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGTTCCTCTATCTACTGGGCACCTTCTCT                    <  SRR4302534.6477414/94‑1 (MQ=60)
                                                                           GATGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGTTCCTCTATCTACTGGGCACCTTCTCT                    <  SRR4302534.4671522/94‑1 (MQ=60)
                                                                             TGCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGTTCCTCTATCTACTGGGCACCTTCTCTGC                  <  SRR4302534.41029/94‑1 (MQ=60)
                                                                              GCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGTTCCTCTATCTACTGGGCACCTTCTCTGCT                 <  SRR4302534.6160362/94‑1 (MQ=60)
                                                                              GCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGTTCCTCTATCTACTGGGCACCTTCTCTGCT                 <  SRR4302534.1348077/94‑1 (MQ=60)
                                                                              GCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGTTCCTCTATCTACTGGGCACCTTCTCTGCT                 <  SRR4302534.5480745/94‑1 (MQ=60)
                                                                              GCTGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGTTCCTCTATCTACTGGGCACCTTCTCTGCT                 <  SRR4302534.5737862/94‑1 (MQ=60)
                                                                                TGGTGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGTTCCTCTATCTACTGGGCACCTTCTCTGCTGC               >  SRR4302534.5921583/1‑94 (MQ=60)
                                                                                   TGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGTTCCTCTATCTACTGGGCACCTTCTCTGCTGCTCT            >  SRR4302534.2585932/1‑94 (MQ=60)
                                                                                   TGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGTTCCTCTATCTACTGGGCACCTTCTCTGCTGCTCT            <  SRR4302534.4932429/94‑1 (MQ=60)
                                                                                   TGATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGTTCCTCTATCTACTGGGCACCTTCTCTGCTGCTCT            >  SRR4302534.5906007/1‑94 (MQ=60)
                                                                                    GATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGTTCCTCTATCTACTGGGCACCTTCTCTGCTGCTCTG           >  SRR4302534.5634558/1‑94 (MQ=60)
                                                                                     ATGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGTTCCTCTATCTACTGGGCACCTTCTCTGCTGCTCTGG          >  SRR4302534.538751/1‑94 (MQ=60)
                                                                                      TGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGTTCCTCTATCTACTGGGCACCTTCTCTGCTGCTCTGGC         >  SRR4302534.2411078/1‑94 (MQ=60)
                                                                                      TGGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGTTCCTCTATCTACTGGGCACCTTCTCTGCTGCTCTGGC         >  SRR4302534.1742224/1‑94 (MQ=60)
                                                                                       GGCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGTTCCTCTATCTACTGGGCACCTTCTCTGCTGCTCTGGCC        <  SRR4302534.1209062/94‑1 (MQ=60)
                                                                                        GCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGTTCCTCTATCTACTGGGCACCTTCTCTGCTGCTCTGGCCG       >  SRR4302534.6502754/1‑94 (MQ=60)
                                                                                        GCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGTTCCTCTATCTACTGGGCACCTTCTCTGCTGCTCTGGCCG       <  SRR4302534.3967468/94‑1 (MQ=60)
                                                                                        GCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGTTCCTCTATCTACTGGGCACCTTCTCTGCTGCTCTGGCCG       >  SRR4302534.4637698/1‑94 (MQ=60)
                                                                                        GCATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGTTCCTCTATCTACTGGGCACCTTCTCTGCTGCTCTGGCCG       <  SRR4302534.2509596/94‑1 (MQ=60)
                                                                                         CATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGTTCCTCTATCTACTGGGCACCTTCTCTGCTGCTCTGGCCGC      >  SRR4302534.3819459/1‑94 (MQ=60)
                                                                                         CATCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGTTCCTCTATCTACTGGGCACCTTCTCTGCTGCTCTGGCCGC      >  SRR4302534.5845132/1‑94 (MQ=60)
                                                                                           TCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGTTCCTCTATCTACTGGGCACCTTCTCTGCTGCTCTGGCCGCAG    >  SRR4302534.6429111/1‑94 (MQ=60)
                                                                                           TCCATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGTTCCTCTATCTACTGGGCACCTTCTCTGCTGCTCTGGCCGCAG    >  SRR4302534.5985525/1‑94 (MQ=60)
                                                                                             CATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGTTCCTCTATCTACTGGGCACCTTCTCTGCTGCTCTGGCCGCAGTA  <  SRR4302534.2828034/94‑1 (MQ=60)
                                                                                             |                                                                                             
CGCGGCGGAAGCTGTTGGTCTGTTAGGTACTTTGTTCGTCGGCGCACTGAAAGCCGTTGCCCCCATCCTGGTGTTGATGCTGGTGATGGCATCTATTGCTAACCACCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGTTCCTCTATCTACTGGGCACCTTCTCTGCTGCTCTGGCCGCAGTA  >  NC_000913/3237697‑3237883

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑