Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 744 338.8 17650688 97.8% 17262372 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,240,123 T→C M278V (ATG→GTG)  uxaA ← altronate hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,240,1230TC98.7% 1051.0 / 1.0 311M278V (ATG→GTG) uxaAaltronate hydrolase
Reads supporting (aligned to +/- strand):  ref base T (3/1);  new base C (153/154);  total (156/155)
Fisher's exact test for biased strand distribution p-value = 6.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.38e-01

Only 100 of 319 total aligned reads displayed.

AGCAACTGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCATCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTCGCGTTTAT  >  NC_000913/3240032‑3240212
                                                                                           |                                                                                         
aGCAACTGCTCTGCGCCAAACATCTCCGGCACTTCGGGCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCATc                                                                                          <  1:1466048/93‑1 (MQ=255)
 gCAACTGCTCTGCGCCAAACATCTCCGTCACTTCGGTCACTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCg                                                                                         <  2:4494370/93‑1 (MQ=255)
 gCAACTGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCg                                                                                         >  1:4187554/1‑93 (MQ=255)
 gCAACTGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCg                                                                                         <  2:7240920/93‑1 (MQ=255)
  cAACTGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCgg                                                                                        <  2:5438957/93‑1 (MQ=255)
  cAACTGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCgg                                                                                        <  1:6864233/93‑1 (MQ=255)
  cAACTGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCgg                                                                                        <  2:6365924/93‑1 (MQ=255)
    aCTGCTCTGCGCCAAACATCTCCGGCGCTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGt                                                                                      <  2:508765/93‑1 (MQ=255)
     cTGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGtt                                                                                     >  2:1388230/1‑93 (MQ=255)
      tGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTa                                                                                    >  2:4671674/1‑93 (MQ=255)
         tctGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCa                                                                                 <  2:2842734/93‑1 (MQ=255)
         tctGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCa                                                                                 <  2:1317836/93‑1 (MQ=255)
          ctGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAg                                                                                >  2:7739294/1‑93 (MQ=255)
           tGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGt                                                                               >  2:4204870/1‑93 (MQ=255)
            gcgcCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTa                                                                              >  2:7890263/1‑93 (MQ=255)
              gcCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAAt                                                                            <  2:8081001/93‑1 (MQ=255)
                  aaCATCTCCGGCACTTCGTGCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCa                                                                        <  1:1901033/93‑1 (MQ=255)
                  aaCATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCa                                                                        <  2:3796570/93‑1 (MQ=255)
                   aCATCTCCGGCACTTCGGTCCGTTCGGGTGTCCCGCCGGTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAg                                                                       <  1:6911617/93‑1 (MQ=255)
                    cATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGa                                                                      >  2:5265535/1‑93 (MQ=255)
                     aTCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACACCCCAGCACCGGGTTAGCAGTAATACCAGaa                                                                     >  2:8590277/1‑93 (MQ=255)
                      tctcCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGaaa                                                                    <  2:5480302/93‑1 (MQ=255)
                       ctcCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACATAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAg                                                                   <  2:2659254/93‑1 (MQ=255)
                        cccGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGa                                                                  <  2:6228290/92‑1 (MQ=255)
                         ccGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGAc                                                                 <  2:5722615/93‑1 (MQ=255)
                             cACTTCGGTCGTTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCGCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTc                                                             <  2:1709555/93‑1 (MQ=255)
                             cACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTc                                                             <  1:2847162/93‑1 (MQ=255)
                              aCTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCa                                                            >  2:1047620/1‑93 (MQ=255)
                               cTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAg                                                           <  2:836776/93‑1 (MQ=255)
                               cTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAg                                                           >  1:3015189/1‑93 (MQ=255)
                                ttCGGTCAGTTCGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGATACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGa                                                          >  1:7835919/1‑93 (MQ=255)
                                 tCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGaa                                                         <  2:1750542/93‑1 (MQ=255)
                                  cGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAac                                                        <  1:1757752/93‑1 (MQ=255)
                                   ggTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAacc                                                       >  1:8075598/1‑93 (MQ=255)
                                     tCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGATTAGCAGTAATACCAGAAAGACCGTCAGAaccac                                                     <  2:8735594/93‑1 (MQ=255)
                                      cAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAaccacc                                                    >  1:6508500/1‑93 (MQ=255)
                                      cAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAaccacc                                                    <  2:6528406/93‑1 (MQ=255)
                                       aGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCg                                                   <  1:2136359/93‑1 (MQ=255)
                                        gTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGc                                                  >  1:5247275/1‑93 (MQ=255)
                                          aCGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCAc                                                <  1:1225187/93‑1 (MQ=255)
                                          aCGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCAc                                                >  2:592735/1‑93 (MQ=255)
                                          aCGGTAGTACAGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCAc                                                >  2:3997363/1‑93 (MQ=255)
                                           cGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACt                                               >  1:1980466/1‑93 (MQ=255)
                                           cGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACt                                               <  1:3225027/93‑1 (MQ=255)
                                             gtagtaCCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCATCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTcc                                             >  1:1853362/1‑93 (MQ=255)
                                             gtagtaCCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTcc                                             <  1:2992417/93‑1 (MQ=255)
                                             gtagtaCCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTcc                                             >  1:8497975/1‑93 (MQ=255)
                                              tagtaCCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCa                                            <  2:753969/93‑1 (MQ=255)
                                                gtaCCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGa                                          >  2:4965014/1‑93 (MQ=255)
                                                  aCCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGAcc                                        <  2:5331523/93‑1 (MQ=255)
                                                   ccgccgTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCa                                       >  1:6391132/1‑93 (MQ=255)
                                                      ccgTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAAc                                    <  2:7305237/93‑1 (MQ=255)
                                                        gTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACtt                                  >  1:4882619/1‑93 (MQ=255)
                                                        gTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACtt                                  <  2:3501076/93‑1 (MQ=255)
                                                        gTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACtt                                  >  1:7172177/1‑93 (MQ=255)
                                                        gTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACAGCACTCCAGACCAAACtt                                  >  1:4417848/1‑93 (MQ=255)
                                                         ttAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTc                                 <  1:7342912/93‑1 (MQ=255)
                                                          tAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCCCTCCAGACCAAACTTCa                                <  2:6860232/93‑1 (MQ=255)
                                                           agcaagcaCGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAg                               <  2:8563081/93‑1 (MQ=255)
                                                           aGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAg                               <  2:7122950/93‑1 (MQ=255)
                                                            gCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGt                              >  1:4339015/1‑93 (MQ=255)
                                                            gCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGt                              >  2:7864822/1‑93 (MQ=255)
                                                            gCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGt                              <  1:7726376/93‑1 (MQ=255)
                                                             cAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGtt                             <  1:4679049/93‑1 (MQ=255)
                                                             cAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGtt                             <  1:8203390/93‑1 (MQ=255)
                                                              gaTCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTc                            >  1:2716329/2‑93 (MQ=255)
                                                              aaTCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTc                            >  1:314753/1‑93 (MQ=255)
                                                              aaTCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTc                            >  2:7615350/1‑93 (MQ=255)
                                                              aaTCACGTAGTCAGAGAAACGCCCCAGCACCGGATTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTc                            <  2:2778405/93‑1 (MQ=255)
                                                              aaTCACGCAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTc                            >  2:7430379/1‑93 (MQ=255)
                                                               aTCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCg                           >  2:3035679/1‑93 (MQ=255)
                                                               aTCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCg                           <  2:6134404/93‑1 (MQ=255)
                                                                tCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGc                          <  1:3992098/93‑1 (MQ=255)
                                                                  aCGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTg                        >  1:3592814/1‑93 (MQ=255)
                                                                    gTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAg                      >  1:3841468/1‑93 (MQ=255)
                                                                     tAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGt                     <  2:4636384/93‑1 (MQ=255)
                                                                     tAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGt                     >  1:6198207/1‑93 (MQ=255)
                                                                      aGTCATAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGtt                    <  1:1019869/93‑1 (MQ=255)
                                                                      aGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGtt                    <  2:1423669/93‑1 (MQ=255)
                                                                      aGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGtt                    <  1:7243652/93‑1 (MQ=255)
                                                                      aGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTCCGCTGAGtt                    <  1:2329838/93‑1 (MQ=255)
                                                                       gTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGttt                   <  1:239693/93‑1 (MQ=255)
                                                                          agagAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGcc                >  1:1082884/1‑93 (MQ=255)
                                                                          agagAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGcc                <  2:2259322/93‑1 (MQ=255)
                                                                           gagaAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGCCCAAACTTCAGTTCGCTGAGTTTGCCt               >  1:7511911/1‑93 (MQ=255)
                                                                           gagaAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCt               >  2:2103360/1‑93 (MQ=255)
                                                                           gagaAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCt               <  1:2430461/93‑1 (MQ=255)
                                                                           gagaAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCt               >  1:5515051/1‑93 (MQ=255)
                                                                            agaAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTg              <  1:3523598/93‑1 (MQ=255)
                                                                             gaAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTgg             >  2:3269253/1‑93 (MQ=255)
                                                                             gaAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTgg             >  1:1588086/1‑93 (MQ=255)
                                                                             gaAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTgg             >  1:6739312/1‑93 (MQ=255)
                                                                               aaCGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCt           <  1:8747226/93‑1 (MQ=255)
                                                                                  gCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCAACGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTcgc        >  1:1176939/1‑93 (MQ=255)
                                                                                   ccccAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTcgcg       >  1:5912096/1‑93 (MQ=255)
                                                                                   ccccAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTcgcg       >  2:5527766/1‑93 (MQ=255)
                                                                                       aGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTCGCGTTTa   >  1:898513/1‑93 (MQ=255)
                                                                                        gCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTCGCGTTTAt  >  1:5749209/1‑93 (MQ=255)
                                                                                        gCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTCGCGTTTAt  <  1:7635342/93‑1 (MQ=255)
                                                                                        gCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTCGCGTTTAt  >  1:4473033/1‑93 (MQ=255)
                                                                                           |                                                                                         
AGCAACTGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCATCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTCGCGTTTAT  >  NC_000913/3240032‑3240212

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TCAGCAACTGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCATCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTCGCGTTTATCGTT  >  NC_000913/3240030‑3240216
                                                                                             |                                                                                             
TCAGCAACTGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCAC                                                                                               <  SRR4302541.8674945/94‑1 (MQ=60)
TCAGCAACTGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCAC                                                                                               <  SRR4302541.8674944/94‑1 (MQ=60)
TCAGCAACTGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCAC                                                                                               >  SRR4302541.4088872/1‑94 (MQ=60)
 CAGCAACTGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACC                                                                                              <  SRR4302541.2373717/94‑1 (MQ=60)
 CAGCAACTGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACC                                                                                              <  SRR4302541.2847374/94‑1 (MQ=60)
 CAGCAACTGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACC                                                                                              <  SRR4302541.8270485/94‑1 (MQ=60)
 CAGCAACTGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACC                                                                                              <  SRR4302541.1196521/94‑1 (MQ=60)
 CAGCAACTGCTCTGCGCCAAACATCTCCGGCACTTCGGGCAGTGCGGTAGTACCGCCGTTAGGAATCACGTAGTCAGAGAAACGCCCCAGCACC                                                                                              <  SRR4302541.3023242/94‑1 (MQ=60)
  AGCAACTGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCG                                                                                             >  SRR4302541.4473388/1‑94 (MQ=60)
  AGCAACTGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCG                                                                                             >  SRR4302541.4229119/1‑94 (MQ=60)
  AGCAACTGCTCTGCGCCAAACATCTCCGGCACTTCGGGCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCATCG                                                                                             <  SRR4302541.1480406/94‑1 (MQ=60)
   GCAACTGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGG                                                                                            <  SRR4302541.4894816/94‑1 (MQ=60)
   GCAACTGCTCTGCGCCAAACATCTCCGGCACTTCGATCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGG                                                                                            <  SRR4302541.2037606/94‑1 (MQ=60)
    CAACTGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGG                                                                                           >  SRR4302541.1929018/1‑94 (MQ=60)
    CAACTGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGG                                                                                           >  SRR4302541.5018544/1‑94 (MQ=60)
    CAACTGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGG                                                                                           <  SRR4302541.6937565/94‑1 (MQ=60)
    CAACTGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGG                                                                                           <  SRR4302541.325357/94‑1 (MQ=60)
      ACTGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTT                                                                                         >  SRR4302541.2323024/1‑94 (MQ=60)
      ACTGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTT                                                                                         <  SRR4302541.7990458/94‑1 (MQ=60)
       CTGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTA                                                                                        >  SRR4302541.4306065/1‑94 (MQ=60)
       CTGCTCTGCCCCCAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTA                                                                                        <  SRR4302541.6534884/94‑1 (MQ=60)
        TGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAG                                                                                       >  SRR4302541.5502680/1‑94 (MQ=60)
        TGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAG                                                                                       >  SRR4302541.3914534/1‑94 (MQ=60)
         GCTCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGC                                                                                      >  SRR4302541.3143266/1‑94 (MQ=60)
          CTCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCA                                                                                     >  SRR4302541.4502947/1‑94 (MQ=60)
          CTCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGCTAGCA                                                                                     >  SRR4302541.6901188/1‑94 (MQ=60)
           TCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAG                                                                                    >  SRR4302541.55612/1‑94 (MQ=60)
           TCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAG                                                                                    >  SRR4302541.461360/1‑94 (MQ=60)
              GCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAA                                                                                 >  SRR4302541.6514889/1‑94 (MQ=60)
                GCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATA                                                                               <  SRR4302541.2744788/94‑1 (MQ=60)
                   AAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCA                                                                            >  SRR4302541.2592667/1‑94 (MQ=60)
                   AAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATAACA                                                                            >  SRR4302541.7818639/1‑94 (MQ=60)
                    AACCTCCCCGGCAATTCGGTCAGTACGGTAGTCACCCCAGTAGCAATCGCGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAG                                                                           <  SRR4302541.6600617/94‑1 (MQ=48)
                    AACATCTCCGGCACTTCGTGCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAG                                                                           <  SRR4302541.1919286/94‑1 (MQ=60)
                    AACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAG                                                                           <  SRR4302541.6600622/94‑1 (MQ=60)
                    AACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGCCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAG                                                                           >  SRR4302541.2483377/1‑94 (MQ=60)
                     ACATCTCCGGCACTTCGGTCCGTTCGGGTGTCCCGCCGGTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGA                                                                          <  SRR4302541.6985369/94‑1 (MQ=60)
                     ACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGA                                                                          <  SRR4302541.6985368/94‑1 (MQ=60)
                     ACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGA                                                                          >  SRR4302541.7002556/1‑94 (MQ=60)
                        TCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAG                                                                       <  SRR4302541.1193253/94‑1 (MQ=60)
                        TCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAG                                                                       <  SRR4302541.2412179/94‑1 (MQ=60)
                          TCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAACCGCCCCAGCCCCGGGTTAGCAGTAATACCAGAAAGAC                                                                     <  SRR4302541.440804/94‑1 (MQ=60)
                          TCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGAC                                                                     >  SRR4302541.7425751/1‑94 (MQ=60)
                           CCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACC                                                                    <  SRR4302541.5826029/94‑1 (MQ=60)
                           CCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACC                                                                    <  SRR4302541.2448008/94‑1 (MQ=60)
                           CCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACC                                                                    <  SRR4302541.4163137/94‑1 (MQ=60)
                              GCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTC                                                                 <  SRR4302541.1687096/94‑1 (MQ=60)
                               CACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCA                                                                <  SRR4302541.2875792/94‑1 (MQ=60)
                               CACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCAAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCA                                                                >  SRR4302541.4487945/1‑94 (MQ=60)
                                ACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAG                                                               >  SRR4302541.3045621/1‑94 (MQ=60)
                                ACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAG                                                               >  SRR4302541.4899080/1‑94 (MQ=60)
                                 ctgcgtaCAGTACGGTACTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGA                                                              <  SRR4302541.5395740/87‑1 (MQ=60)
                                 CTTCGGTCAGTTCGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGATACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGA                                                              >  SRR4302541.7921378/1‑94 (MQ=60)
                                 CTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGCAAGACCGTCAGA                                                              >  SRR4302541.7361835/1‑94 (MQ=60)
                                 CTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGA                                                              <  SRR4302541.765571/94‑1 (MQ=60)
                                 CTTCGGGCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGA                                                              <  SRR4302541.2427518/94‑1 (MQ=60)
                                    CGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACC                                                           >  SRR4302541.8164220/1‑94 (MQ=60)
                                    CGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACC                                                           <  SRR4302541.2122025/94‑1 (MQ=60)
                                    CGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACC                                                           <  SRR4302541.1774876/94‑1 (MQ=60)
                                    CGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGACAGAACC                                                           >  SRR4302541.7854824/1‑94 (MQ=60)
                                     GGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCC                                                          >  SRR4302541.2222785/1‑94 (MQ=60)
                                     GGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCA                                                          <  SRR4302541.410696/94‑1 (MQ=60)
                                      GTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCAC                                                         >  SRR4302541.6041858/1‑94 (MQ=60)
                                       TCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACC                                                        >  SRR4302541.6574896/1‑94 (MQ=60)
                                        CAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCG                                                       <  SRR4302541.3039031/94‑1 (MQ=60)
                                        CAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCG                                                       <  SRR4302541.3656717/94‑1 (MQ=60)
                                         AGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGC                                                      <  SRR4302541.7779088/94‑1 (MQ=60)
                                         AGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGC                                                      <  SRR4302541.2157018/94‑1 (MQ=60)
                                         AGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGC                                                      >  SRR4302541.5301344/1‑94 (MQ=60)
                                         AGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGC                                                      <  SRR4302541.3006349/94‑1 (MQ=60)
                                            ACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACT                                                   >  SRR4302541.1999598/1‑94 (MQ=60)
                                            ACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACT                                                   <  SRR4302541.1237201/94‑1 (MQ=60)
                                            ACAGTAGTACCGCCGTTAGCAATCACGTAGTCAGATAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAAACACCGCACT                                                   <  SRR4302541.3040625/94‑1 (MQ=60)
                                             CGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTC                                                  <  SRR4302541.312310/94‑1 (MQ=60)
                                             CGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTC                                                  <  SRR4302541.3257598/94‑1 (MQ=60)
                                              GGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCATCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCC                                                 >  SRR4302541.1871270/1‑94 (MQ=60)
                                              GGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCC                                                 >  SRR4302541.8589511/1‑94 (MQ=60)
                                               GTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCA                                                <  SRR4302541.3022623/94‑1 (MQ=60)
                                                  GTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGAC                                             <  SRR4302541.3683893/94‑1 (MQ=60)
                                                   TACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACC                                            >  SRR4302541.1580432/1‑94 (MQ=60)
                                                    ACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCA                                           >  SRR4302541.6456377/1‑94 (MQ=60)
                                                      CGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAA                                         <  SRR4302541.1326943/94‑1 (MQ=60)
                                                       GCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAAC                                        <  SRR4302541.1268246/94‑1 (MQ=60)
                                                       GCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAAC                                        >  SRR4302541.7507455/1‑94 (MQ=60)
                                                        CCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACT                                       >  SRR4302541.7381721/1‑94 (MQ=60)
                                                         CGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTT                                      >  SRR4302541.7281070/1‑94 (MQ=60)
                                                         CGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTT                                      >  SRR4302541.4932443/1‑94 (MQ=60)
                                                         CGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTT                                      >  SRR4302541.7248644/1‑94 (MQ=60)
                                                         CGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACAGCACTCCAGACCAAACTT                                      >  SRR4302541.4462168/1‑94 (MQ=60)
                                                          GTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTC                                     >  SRR4302541.3542337/1‑94 (MQ=60)
                                                          GTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTC                                     >  SRR4302541.4461405/1‑94 (MQ=60)
                                                           TTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCA                                    <  SRR4302541.7421342/94‑1 (MQ=60)
                                                           TTAGCAATCACGTAGTCAGAGAAACGCACCAGCACCGGCTTAGCAGCACTAACAGAAAGACCGTCAGAACCACAGCACTCCAGACAAAACTTCA                                    >  SRR4302541.3834783/1‑94 (MQ=60)
                                                            TAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAG                                   >  SRR4302541.8257720/1‑94 (MQ=60)
                                                            TAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGAACAAACTTCAG                                   >  SRR4302541.1895323/1‑94 (MQ=60)
                                                             AGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGT                                  >  SRR4302541.4382339/1‑94 (MQ=60)
                                                              GCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTT                                 <  SRR4302541.8874839/94‑1 (MQ=60)
                                                              GCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTT                                 <  SRR4302541.7808577/94‑1 (MQ=60)
                                                               CGATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTC                                >  SRR4302541.2743417/1‑94 (MQ=60)
                                                               CAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTC                                <  SRR4302541.8292900/94‑1 (MQ=60)
                                                               CAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTC                                <  SRR4302541.4726708/94‑1 (MQ=60)
                                                               CAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTC                                >  SRR4302541.317545/1‑94 (MQ=60)
                                                                AATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGTAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCG                               >  SRR4302541.5521844/1‑94 (MQ=60)
                                                                AATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCG                               <  SRR4302541.6687303/94‑1 (MQ=60)
                                                                AATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCG                               <  SRR4302541.1117207/94‑1 (MQ=60)
                                                                AATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCG                               >  SRR4302541.6941765/1‑94 (MQ=60)
                                                                  TCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCT                             <  SRR4302541.5752967/94‑1 (MQ=60)
                                                                  TCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCT                             <  SRR4302541.4031635/94‑1 (MQ=60)
                                                                  TCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCT                             <  SRR4302541.514148/94‑1 (MQ=60)
                                                                   CACGTAGTCAGAGAAACGCCCCAGGACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTG                            >  SRR4302541.7451384/1‑94 (MQ=60)
                                                                   CACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTG                            >  SRR4302541.3629244/1‑94 (MQ=60)
                                                                   CACGTAGTCAGAGAAACGCCCCAGAACCGGGTTAGGAGTAATACCAGAAAGCCCGTCAGATCCACCGCACTCCAGACCAAACTTCAGTTCGCTG                            <  SRR4302541.3010370/94‑1 (MQ=60)
                                                                     CGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAG                          <  SRR4302541.612409/94‑1 (MQ=60)
                                                                     CGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAG                          >  SRR4302541.3880158/1‑94 (MQ=60)
                                                                      GTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGT                         >  SRR4302541.6261544/1‑94 (MQ=60)
                                                                      GTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGT                         >  SRR4302541.1744918/1‑94 (MQ=60)
                                                                      GTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGT                         >  SRR4302541.4676953/1‑94 (MQ=60)
                                                                        AGTCATAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTT                       <  SRR4302541.1029290/94‑1 (MQ=60)
                                                                        AGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTT                       <  SRR4302541.7319844/94‑1 (MQ=60)
                                                                        AGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTT                       <  SRR4302541.7320911/94‑1 (MQ=60)
                                                                        AGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTT                       >  SRR4302541.22938/1‑94 (MQ=60)
                                                                        AGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTCCGCTGAGTTT                       <  SRR4302541.2352456/94‑1 (MQ=60)
                                                                         GTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTG                      <  SRR4302541.1227071/94‑1 (MQ=60)
                                                                         GTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTG                      <  SRR4302541.241603/94‑1 (MQ=60)
                                                                         GTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTG                      >  SRR4302541.1897535/1‑94 (MQ=60)
                                                                          TCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGC                     <  SRR4302541.165068/94‑1 (MQ=60)
                                                                           CAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCC                    >  SRR4302541.1092988/1‑94 (MQ=60)
                                                                            AGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGCCCAAACTTCAGTTCGCTGAGTTTGCCT                   >  SRR4302541.7592199/1‑94 (MQ=60)
                                                                            AGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCT                   <  SRR4302541.5659722/94‑1 (MQ=60)
                                                                            AGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCT                   >  SRR4302541.5571507/1‑94 (MQ=60)
                                                                            AGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCT                   >  SRR4302541.3628608/1‑94 (MQ=60)
                                                                            AGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCT                   >  SRR4302541.5852898/1‑94 (MQ=60)
                                                                            AGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCT                   >  SRR4302541.3924408/1‑94 (MQ=60)
                                                                             GAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTG                  <  SRR4302541.886983/94‑1 (MQ=60)
                                                                             GAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTG                  <  SRR4302541.2454505/94‑1 (MQ=60)
                                                                             GAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTG                  <  SRR4302541.6835915/94‑1 (MQ=60)
                                                                              AGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGG                 >  SRR4302541.6811255/1‑94 (MQ=60)
                                                                              AGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGG                 >  SRR4302541.2416051/1‑94 (MQ=60)
                                                                              AGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGG                 <  SRR4302541.8314378/94‑1 (MQ=60)
                                                                              AGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGG                 >  SRR4302541.1603911/1‑94 (MQ=60)
                                                                              AGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGG                 >  SRR4302541.1035179/1‑94 (MQ=60)
                                                                              AGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGG                 <  SRR4302541.3559057/94‑1 (MQ=60)
                                                                              AGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGG                 >  SRR4302541.8780822/1‑94 (MQ=60)
                                                                               GAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGC                >  SRR4302541.7099729/1‑94 (MQ=60)
                                                                                AAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGCCCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCT               <  SRR4302541.7648607/94‑1 (MQ=60)
                                                                                AAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCT               <  SRR4302541.8239915/94‑1 (MQ=60)
                                                                                AAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCT               >  SRR4302541.8693004/1‑94 (MQ=60)
                                                                                AAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGCCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCT               <  SRR4302541.7735489/94‑1 (MQ=60)
                                                                                 AACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTC              >  SRR4302541.8230877/1‑94 (MQ=60)
                                                                                 AACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTC              <  SRR4302541.8840814/94‑1 (MQ=60)
                                                                                   CGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTCGC            >  SRR4302541.1178488/1‑94 (MQ=60)
                                                                                   CGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTCGC            <  SRR4302541.1063242/94‑1 (MQ=60)
                                                                                   CGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCAACGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTCGC            >  SRR4302541.1188303/1‑94 (MQ=60)
                                                                                    GCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTCGCG           >  SRR4302541.1007178/1‑94 (MQ=60)
                                                                                    GCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTCGCG           <  SRR4302541.3255858/94‑1 (MQ=60)
                                                                                    GCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTCGCG           >  SRR4302541.5972275/1‑94 (MQ=60)
                                                                                    GCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTCGCG           >  SRR4302541.853622/1‑94 (MQ=60)
                                                                                    GCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTCGCG           >  SRR4302541.8045411/1‑94 (MQ=60)
                                                                                    GCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTCGCG           >  SRR4302541.2799782/1‑94 (MQ=60)
                                                                                    GCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTCGCG           >  SRR4302541.2905916/1‑94 (MQ=60)
                                                                                      CCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTCGCGTT         <  SRR4302541.6323441/94‑1 (MQ=60)
                                                                                      CCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTCGCGTT         >  SRR4302541.1806345/1‑94 (MQ=60)
                                                                                       CCAGCACCGGGTTAGCAGTAATACCAGAAAGGCCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTCGCGTTT        <  SRR4302541.1808458/94‑1 (MQ=60)
                                                                                       CCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTCGCGTTT        <  SRR4302541.1530619/94‑1 (MQ=60)
                                                                                        CAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTCGCGTTTA       >  SRR4302541.906821/1‑94 (MQ=60)
                                                                                        CAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTCGCGTTTA       >  SRR4302541.3868657/1‑94 (MQ=60)
                                                                                         AGCATCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTCGCGTTTAT      >  SRR4302541.3593518/1‑94 (MQ=60)
                                                                                         AGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTCGCGTTTAT      >  SRR4302541.5807751/1‑94 (MQ=60)
                                                                                         AGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTCGCGTTTAT      >  SRR4302541.1993600/1‑94 (MQ=60)
                                                                                         AGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTCGCGTTTAT      <  SRR4302541.3301749/94‑1 (MQ=60)
                                                                                         AGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTCGCGTTTAT      <  SRR4302541.6411448/94‑1 (MQ=60)
                                                                                         AGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTCGCGTTTAT      >  SRR4302541.4518104/1‑94 (MQ=60)
                                                                                         AGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTCGCGTTTAT      <  SRR4302541.4259233/94‑1 (MQ=60)
                                                                                          GCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTCGCGTTTATC     <  SRR4302541.7716784/94‑1 (MQ=60)
                                                                                           CACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACGGAACTCCAGACCAACGTCCAGTTCCCGCAGTTTGCCTGGCCGGCGTTTATCG    >  SRR4302541.6864937/1‑94 (MQ=40)
                                                                                           CACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTCGCGTTTATCG    >  SRR4302541.6864936/1‑94 (MQ=60)
                                                                                           CACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTCGCGTTTATCG    <  SRR4302541.3977664/94‑1 (MQ=60)
                                                                                           CACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTCGCGTTTATCG    >  SRR4302541.179459/1‑94 (MQ=60)
                                                                                            ACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTCGCGTTTATCGT   <  SRR4302541.4183883/94‑1 (MQ=60)
                                                                                            ACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTCGCGTTTATCGT   >  SRR4302541.5813551/1‑94 (MQ=60)
                                                                                             CCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTCGCGTTTATCGTT  <  SRR4302541.4372578/94‑1 (MQ=60)
                                                                                             CCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTCGCGTTTATCGTT  <  SRR4302541.3366025/94‑1 (MQ=60)
                                                                                             CCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTCGCGTTTATCGTT  <  SRR4302541.1413130/94‑1 (MQ=60)
                                                                                             CCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTCGCGTTTATCGTT  >  SRR4302541.4989833/1‑94 (MQ=60)
                                                                                             CCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTCGCGTTTATCGTT  >  SRR4302541.5320269/1‑94 (MQ=60)
                                                                                             CCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTCGCGTTTATCGTT  >  SRR4302541.5892196/1‑94 (MQ=60)
                                                                                             |                                                                                             
TCAGCAACTGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCATCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTCGCGTTTATCGTT  >  NC_000913/3240030‑3240216

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑