Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	884	257.2	12958910	96.9%	12557183	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,240,123	T→C	M278V (ATG→GTG)	uxaA ←	altronate hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,240,123	0	T	C	100.0%	795.5 / NA	228	M278V (ATG→GTG)	uxaA	altronate hydrolase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (132/96); total (132/96)

Only 100 of 230 total aligned reads displayed.

GCAACTGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCATCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTCGCGTTTAT  >  NC_000913/3240033‑3240212

gCAACTGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCg                                                                                         >  2:2545670/1‑93 (MQ=255)

gCAACTGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCg                                                                                         >  2:2825802/1‑93 (MQ=255)

gCAACTGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCg                                                                                         >  1:3624737/1‑93 (MQ=255)

gCAACTGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCg                                                                                         >  1:3456501/1‑93 (MQ=255)

   aCTGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGt                                                                                      >  2:4456466/1‑93 (MQ=255)

   aCTGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGt                                                                                      <  1:2283319/93‑1 (MQ=255)

   aCTGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCACAGCACCGGGt                                                                                      >  1:6066036/1‑93 (MQ=255)

    cTGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGtt                                                                                     <  2:547738/93‑1 (MQ=255)

     tGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTa                                                                                    >  2:4906115/1‑93 (MQ=255)

        tctGCGCCAAACATCTCCGACACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACTCCCCAGCACCGGGTTAGCa                                                                                 <  1:6289172/93‑1 (MQ=255)

         ctGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAg                                                                                <  1:695896/93‑1 (MQ=255)

           gcgcCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTa                                                                              >  1:594191/1‑93 (MQ=255)

           gcgcCAAACATCTCCGGCACTTAGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTa                                                                              <  2:1959452/93‑1 (MQ=255)

            cgcCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTaa                                                                             >  1:2790624/1‑93 (MQ=255)

             gcCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAAt                                                                            <  1:2115058/93‑1 (MQ=255)

               cAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATAc                                                                          >  2:4306665/1‑93 (MQ=255)

                aaaCATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATAcc                                                                         <  2:5791799/93‑1 (MQ=255)

                aaaCATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATAcc                                                                         >  1:2939022/1‑93 (MQ=255)

                 aaCATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCa                                                                        >  2:5225708/1‑93 (MQ=255)

                  aCATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAg                                                                       <  1:2385060/93‑1 (MQ=255)

                  aCATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAg                                                                       >  1:417722/1‑93 (MQ=255)

                  aCATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAg                                                                       >  1:3264467/1‑93 (MQ=255)

                   cATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGa                                                                      <  2:409887/93‑1 (MQ=255)

                     tctcCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGaaa                                                                    >  1:61903/1‑93 (MQ=255)

                      ctcCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAg                                                                   >  2:6045093/1‑93 (MQ=255)

                       tcCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGTAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGa                                                                  <  2:4661052/93‑1 (MQ=255)

                       tcCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGa                                                                  >  1:167225/1‑93 (MQ=255)

                       tcCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGa                                                                  <  1:5094532/93‑1 (MQ=255)

                        ccGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTATCAGTAATACCAGAAAGAc                                                                 <  1:989893/93‑1 (MQ=255)

                         cGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGAcc                                                                >  1:4840036/1‑93 (MQ=255)

                          ggCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCg                                                               >  1:4107084/1‑93 (MQ=255)

                          ggCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCg                                                               >  1:3228814/1‑93 (MQ=255)

                          ggCACGTCGGTCAGTCCGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATGCCAGAAAggctg                                                               <  1:5701931/93‑5 (MQ=255)

                             aCTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCa                                                            >  1:2972398/1‑93 (MQ=255)

                              cTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAg                                                           <  2:6013882/93‑1 (MQ=255)

                              cTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAg                                                           >  2:2119820/1‑93 (MQ=255)

                               ttCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGa                                                          >  1:2177143/1‑93 (MQ=255)

                                tCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGaa                                                         >  2:4137512/1‑93 (MQ=255)

                                tCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGaa                                                         >  2:4583641/1‑93 (MQ=255)

                                 cGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAac                                                        >  1:3366325/1‑93 (MQ=255)

                                  ggTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAacc                                                       <  2:5951593/93‑1 (MQ=255)

                                    tCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAaccac                                                     <  2:2141518/93‑1 (MQ=255)

                                    tCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAaccac                                                     <  2:3245251/93‑1 (MQ=255)

                                     cAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAaccacc                                                    >  1:1716678/1‑93 (MQ=255)

                                       gTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGc                                                  >  1:807526/1‑93 (MQ=255)

                                         aCGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCAc                                                >  1:5981243/1‑93 (MQ=255)

                                         aCGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCAc                                                <  2:5235130/93‑1 (MQ=255)

                                            gtagtaCCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTcc                                             <  1:3936437/93‑1 (MQ=255)

                                            gtagtaCCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTcc                                             <  1:868128/93‑1 (MQ=255)

                                              agtaCCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAg                                           >  1:5196954/1‑93 (MQ=255)

                                                 aCCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGAcc                                        >  2:823680/1‑93 (MQ=255)

                                                   cgccgTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCaa                                      >  2:6368552/1‑93 (MQ=255)

                                                    gccgTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCaaa                                     >  2:589929/1‑93 (MQ=255)

                                                     ccgTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGACCCACCGCACTCCAGACCAAAc                                    <  2:5130084/93‑1 (MQ=255)

                                                     ccgTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAAc                                    >  1:1067886/1‑93 (MQ=255)

                                                      cgTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACt                                   >  1:4004496/1‑93 (MQ=255)

                                                      cgTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACt                                   >  1:1099086/1‑93 (MQ=255)

                                                      cgTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACt                                   <  1:4783555/93‑1 (MQ=255)

                                                          aGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAg                               <  2:1162316/93‑1 (MQ=255)

                                                          aGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAg                               >  2:2514352/1‑93 (MQ=255)

                                                          aGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAg                               <  2:4188881/93‑1 (MQ=255)

                                                            cAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGtt                             <  2:718708/93‑1 (MQ=255)

                                                            cAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGtt                             >  1:4309799/1‑93 (MQ=255)

                                                             aaTCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTc                            >  1:5837717/1‑93 (MQ=255)

                                                              aTCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCg                           >  2:656080/1‑93 (MQ=255)

                                                                cACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCt                         <  2:3781127/93‑1 (MQ=255)

                                                                cACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCACACCAAGCTTCAGTTCGCt                         >  2:6120038/1‑93 (MQ=255)

                                                                   gTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAg                      >  1:2744966/1‑93 (MQ=255)

                                                                   gTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAg                      >  2:3643083/1‑93 (MQ=255)

                                                                   gTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAg                      >  1:2514087/1‑93 (MQ=255)

                                                                    tAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGt                     <  1:4449913/93‑1 (MQ=255)

                                                                     aGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTTAGtt                    >  1:1993140/1‑93 (MQ=255)

                                                                     aGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGtt                    <  2:4551639/93‑1 (MQ=255)

                                                                     aGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGtt                    <  1:2336525/93‑1 (MQ=255)

                                                                     aGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGtt                    >  2:3843598/1‑93 (MQ=255)

                                                                     aGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGtt                    <  2:1585930/93‑1 (MQ=255)

                                                                      gTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGttt                   >  2:405082/1‑93 (MQ=255)

                                                                         agagAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGcc                <  1:2194760/93‑1 (MQ=255)

                                                                          gagaAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGCCCAAACTTCAGTTCGCTGAGTTTGCCt               <  1:3152220/93‑1 (MQ=255)

                                                                          gagaAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCt               <  1:3103625/93‑1 (MQ=255)

                                                                           agaAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTg              <  2:3005073/93‑1 (MQ=255)

                                                                            gaAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTgg             >  1:6426879/1‑93 (MQ=255)

                                                                            gaAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTgg             <  1:957140/93‑1 (MQ=255)

                                                                            gaAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTgg             <  2:2316955/93‑1 (MQ=255)

                                                                            gaAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTgg             >  1:176315/1‑93 (MQ=255)

                                                                              aaCGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCt           >  1:5458418/1‑93 (MQ=255)

                                                                              aaCGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCt           >  2:1831724/1‑93 (MQ=255)

                                                                              aaCGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCt           <  1:5355125/93‑1 (MQ=255)

                                                                              aaCGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCt           <  2:3326932/93‑1 (MQ=255)

                                                                              aaCGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCt           >  1:3997739/1‑93 (MQ=255)

                                                                                cGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTcg         >  2:5574596/1‑93 (MQ=255)

                                                                                  ccccAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTcgcg       >  1:1270930/1‑93 (MQ=255)

                                                                                   cccAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTCGCGt      >  1:5800333/1‑93 (MQ=255)

                                                                                   cccAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTCGCGt      >  2:2250543/1‑93 (MQ=255)

                                                                                   cccAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTCGCGt      <  2:3979361/93‑1 (MQ=255)

                                                                                    ccAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTCGCGtt     >  1:5251655/1‑93 (MQ=255)

                                                                                     cAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTCGCGttt    >  1:2062220/1‑93 (MQ=255)

                                                                                     cAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTCGCGttt    >  1:1608881/1‑93 (MQ=255)

                                                                                      aGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTCGCGTTTa   >  2:6148807/1‑93 (MQ=255)

                                                                                       gCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTCGCGTTTAt  >  2:1424097/1‑93 (MQ=255)

GCAACTGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCATCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTCGCGTTTAT  >  NC_000913/3240033‑3240212

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TCAGCAACTGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCATCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTCGCGTTTATCGTT  >  NC_000913/3240030‑3240216

TCAGCAACTGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCAC                                                                                               <  SRR4302534.3603924/94‑1 (MQ=60)

 CAGCAACTGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACC                                                                                              >  SRR4302534.2186943/1‑94 (MQ=60)

 CAGCAACTGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACC                                                                                              >  SRR4302534.1662867/1‑94 (MQ=60)

 CAGCAACTGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACC                                                                                              <  SRR4302534.6126379/94‑1 (MQ=60)

  AGCAACTGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCG                                                                                             >  SRR4302534.3722188/1‑94 (MQ=60)

  AGCAACTGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCG                                                                                             >  SRR4302534.3549412/1‑94 (MQ=60)

    CAACTGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGG                                                                                           >  SRR4302534.4050561/1‑94 (MQ=60)

    CAACTGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGG                                                                                           >  SRR4302534.1909712/1‑94 (MQ=60)

     AACTGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCACAGCACCGGGT                                                                                          >  SRR4302534.6230960/1‑94 (MQ=60)

      ACTGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTT                                                                                         >  SRR4302534.2147555/1‑94 (MQ=60)

      ACTGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTT                                                                                         <  SRR4302534.2343907/94‑1 (MQ=60)

           TCTGCGCCAAACATCTCCGACACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACTCCCCAGCACCGGGTTAGCAG                                                                                    <  SRR4302534.6459328/94‑1 (MQ=60)

            CTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGT                                                                                   <  SRR4302534.712940/94‑1 (MQ=60)

             TGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTA                                                                                  >  SRR4302534.4110653/1‑94 (MQ=60)

             TGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTA                                                                                  >  SRR4302534.608688/1‑94 (MQ=60)

              GCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAA                                                                                 >  SRR4302534.2864019/1‑94 (MQ=60)

               CGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAAT                                                                                <  SRR4302534.1743988/94‑1 (MQ=60)

                GCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATA                                                                               <  SRR4302534.2170701/94‑1 (MQ=60)

                GCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATA                                                                               >  SRR4302534.2072954/1‑94 (MQ=60)

                GCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATA                                                                               <  SRR4302534.5815217/94‑1 (MQ=60)

                 CCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATAC                                                                              >  SRR4302534.2379274/1‑94 (MQ=60)

                 CCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATAC                                                                              >  SRR4302534.3688479/1‑94 (MQ=60)

                 CCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATAC                                                                              >  SRR4302534.1009511/1‑94 (MQ=60)

                  CAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACC                                                                             >  SRR4302534.3016154/1‑94 (MQ=60)

                  CAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACC                                                                             >  SRR4302534.3033529/1‑94 (MQ=60)

                   AAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCA                                                                            <  SRR4302534.1099199/94‑1 (MQ=60)

                    AACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAG                                                                           >  SRR4302534.2173978/1‑94 (MQ=60)

                    AACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAG                                                                           >  SRR4302534.870937/1‑94 (MQ=60)

                    AACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAG                                                                           <  SRR4302534.3207156/94‑1 (MQ=60)

                    AACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAG                                                                           >  SRR4302534.3351404/1‑94 (MQ=60)

                    AACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAG                                                                           >  SRR4302534.428406/1‑94 (MQ=60)

                     ACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGA                                                                          <  SRR4302534.5366451/94‑1 (MQ=60)

                     ACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGA                                                                          <  SRR4302534.2448431/94‑1 (MQ=60)

                      CATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAA                                                                         <  SRR4302534.2445418/94‑1 (MQ=60)

                       ATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAA                                                                        >  SRR4302534.63591/1‑94 (MQ=60)

                         CTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGA                                                                      >  SRR4302534.171614/1‑94 (MQ=60)

                          TCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGAC                                                                     <  SRR4302534.5232049/94‑1 (MQ=60)

                           CCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTATCAGTAATACCAGAAAGACC                                                                    <  SRR4302534.1015225/94‑1 (MQ=60)

                           CCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACC                                                                    >  SRR4302534.3486523/1‑94 (MQ=60)

                           CCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACC                                                                    <  SRR4302534.2720380/94‑1 (MQ=60)

                           CCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACC                                                                    >  SRR4302534.4971318/1‑94 (MQ=60)

                            CGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCG                                                                   >  SRR4302534.4217545/1‑94 (MQ=60)

                            CGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCG                                                                   >  SRR4302534.3314607/1‑94 (MQ=60)

                            CGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCG                                                                   >  SRR4302534.5092985/1‑94 (MQ=60)

                            CGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCG                                                                   <  SRR4302534.6080655/94‑1 (MQ=60)

                             GGCACGTCGGTCAGTCCGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATGCCAGAAAGgctgt                                                                  <  SRR4302534.5856383/94‑6 (MQ=60)

                               CACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCA                                                                >  SRR4302534.3261578/1‑94 (MQ=60)

                               CACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCA                                                                >  SRR4302534.3050222/1‑94 (MQ=60)

                               CACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCA                                                                >  SRR4302534.5486665/1‑94 (MQ=60)

                               CACTTCGGTCAGTACGGGAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCA                                                                >  SRR4302534.4146245/1‑94 (MQ=60)

                                ACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAG                                                               >  SRR4302534.3083713/1‑94 (MQ=60)

                                ACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAG                                                               <  SRR4302534.4626917/94‑1 (MQ=60)

                                 CTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCATTAATACCAGAAAGACCGTCAGA                                                              >  SRR4302534.3139817/1‑94 (MQ=60)

                                 CTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGA                                                              >  SRR4302534.2234661/1‑94 (MQ=60)

                                 CTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGA                                                              >  SRR4302534.6379199/1‑94 (MQ=60)

                                   TCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAAC                                                            >  SRR4302534.3456509/1‑94 (MQ=60)

                                     GGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCA                                                          <  SRR4302534.3432631/94‑1 (MQ=60)

                                      GTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCAC                                                         >  SRR4302534.2576355/1‑94 (MQ=60)

                                       TCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACC                                                        >  SRR4302534.1761273/1‑94 (MQ=60)

                                        CAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCG                                                       <  SRR4302534.4322602/94‑1 (MQ=60)

                                        CAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGACCCAGCACCTGGTTAGCAGTAATACCAGAAACACCTTCAGAACCACCG                                                       >  SRR4302534.3364608/1‑94 (MQ=60)

                                         AGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGC                                                      >  SRR4302534.827821/1‑94 (MQ=60)

                                         AGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGC                                                      >  SRR4302534.2435033/1‑94 (MQ=60)

                                          GTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCA                                                     <  SRR4302534.6124498/94‑1 (MQ=60)

                                           TACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCAC                                                    >  SRR4302534.6143723/1‑94 (MQ=60)

                                             CGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTC                                                  >  SRR4302534.4229563/1‑94 (MQ=60)

                                             CGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTC                                                  <  SRR4302534.1216663/94‑1 (MQ=60)

                                               GTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCA                                                <  SRR4302534.5516249/94‑1 (MQ=60)

                                               GTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCA                                                <  SRR4302534.890047/94‑1 (MQ=60)

                                               GTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCA                                                <  SRR4302534.4041883/94‑1 (MQ=60)

                                               GTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCA                                                <  SRR4302534.1676212/94‑1 (MQ=60)

                                                TAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAG                                               >  SRR4302534.5337100/1‑94 (MQ=60)

                                                 AGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGA                                              <  SRR4302534.6277905/94‑1 (MQ=60)

                                                    ACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCA                                           >  SRR4302534.1774901/1‑94 (MQ=60)

                                                      tggcgaTAGCAATCACGTAGTCGGAGAAACGACCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAA                                         >  SRR4302534.6566208/7‑94 (MQ=60)

                                                       GCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAAC                                        >  SRR4302534.4115423/1‑94 (MQ=60)

                                                       GCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAAC                                        >  SRR4302534.1095571/1‑94 (MQ=60)

                                                        CCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACT                                       >  SRR4302534.1127717/1‑94 (MQ=60)

                                                        CCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACT                                       >  SRR4302534.4111721/1‑94 (MQ=60)

                                                         CGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTT                                      <  SRR4302534.4913459/94‑1 (MQ=60)

                                                          GTTAGCAATCACTTAGTCAGAGAAAAGCCCCAGCACCGGGCTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGATCAAACTTC                                     >  SRR4302534.127113/1‑94 (MQ=60)

                                                            TAGCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAG                                   <  SRR4302534.3899490/94‑1 (MQ=60)

                                                              GCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTT                                 <  SRR4302534.2857705/94‑1 (MQ=60)

                                                              GCAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTT                                 >  SRR4302534.4426568/1‑94 (MQ=60)

                                                               GAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTC                                <  SRR4302534.6585809/94‑1 (MQ=60)

                                                               CAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTC                                <  SRR4302534.587179/94‑1 (MQ=60)

                                                               CAATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTC                                >  SRR4302534.5996190/1‑94 (MQ=60)

                                                                AATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCG                               <  SRR4302534.4856044/94‑1 (MQ=60)

                                                                AATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCG                               >  SRR4302534.528173/1‑94 (MQ=60)

                                                                AATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCG                               <  SRR4302534.3797421/94‑1 (MQ=60)

                                                                AATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCG                               <  SRR4302534.2222740/94‑1 (MQ=60)

                                                                AATCACGTAGGCAGAGAAACGCCCCAGCACCGGGGTAGCAGTAATACCAGAAAGAACGTCAGAACCACCGCACTCCAGACTAAACATCAGTTCG                               >  SRR4302534.248431/1‑94 (MQ=60)

                                                                 ATCACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGC                              <  SRR4302534.3330327/94‑1 (MQ=60)

                                                                   CACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTG                            >  SRR4302534.903104/1‑94 (MQ=60)

                                                                    ACGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGA                           <  SRR4302534.5967526/94‑1 (MQ=60)

                                                                     CGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAG                          >  SRR4302534.2817268/1‑94 (MQ=60)

                                                                     CGTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAG                          >  SRR4302534.2580835/1‑94 (MQ=60)

                                                                      GTAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGAGCAAACTTCAGTTCGCTGAGT                         >  SRR4302534.1010601/1‑94 (MQ=60)

                                                                       TAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTTAGTT                        >  SRR4302534.2045346/1‑94 (MQ=60)

                                                                       TAGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTT                        <  SRR4302534.4570180/94‑1 (MQ=60)

                                                                        AGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTT                       <  SRR4302534.5330184/94‑1 (MQ=60)

                                                                        AGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTT                       <  SRR4302534.776542/94‑1 (MQ=60)

                                                                        AGTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTT                       <  SRR4302534.2398530/94‑1 (MQ=60)

                                                                         GTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCCGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTG                      <  SRR4302534.6648466/94‑1 (MQ=60)

                                                                         GTCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTG                      <  SRR4302534.4918123/94‑1 (MQ=60)

                                                                          TCAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGC                     >  SRR4302534.3431989/1‑94 (MQ=60)

                                                                           CAGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCC                    >  SRR4302534.1992656/1‑94 (MQ=60)

                                                                            AGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGACCCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCT                   <  SRR4302534.6176873/94‑1 (MQ=60)

                                                                            AGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCT                   <  SRR4302534.5564327/94‑1 (MQ=60)

                                                                            AGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCT                   <  SRR4302534.2252820/94‑1 (MQ=60)

                                                                            AGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCT                   >  SRR4302534.2939459/1‑94 (MQ=60)

                                                                            AGAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCT                   <  SRR4302534.2828513/94‑1 (MQ=60)

                                                                             GAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGCCCAAACTTCAGTTCGCTGAGTTTGCCTG                  <  SRR4302534.3235219/94‑1 (MQ=60)

                                                                             GAGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTG                  <  SRR4302534.3185259/94‑1 (MQ=60)

                                                                              AGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGG                 >  SRR4302534.180953/1‑94 (MQ=60)

                                                                              AGAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGG                 >  SRR4302534.6600195/1‑94 (MQ=60)

                                                                               GAAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGC                <  SRR4302534.981518/94‑1 (MQ=60)

                                                                                AAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCT               >  SRR4302534.5605519/1‑94 (MQ=60)

                                                                                AAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCT               <  SRR4302534.1435013/94‑1 (MQ=60)

                                                                                AAACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCT               >  SRR4302534.4104754/1‑94 (MQ=60)

                                                                                 AACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTC              <  SRR4302534.5499490/94‑1 (MQ=60)

                                                                                  ACGCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTCG             <  SRR4302534.6003040/94‑1 (MQ=60)

                                                                                    GCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTCGCG           >  SRR4302534.1304400/1‑94 (MQ=60)

                                                                                    GCCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTCGCG           >  SRR4302534.168187/1‑94 (MQ=60)

                                                                                     CCCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTCGCGT          >  SRR4302534.5957737/1‑94 (MQ=60)

                                                                                      CCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAAacacccccccccccaccaaacctcaattcacttctgttttctttgtcgcgct         >  SRR4302534.6508246/1‑42 (MQ=60)

                                                                                      CCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTCGCGTT         >  SRR4302534.5393555/1‑94 (MQ=60)

                                                                                      CCCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTCGCGTT         >  SRR4302534.2274804/1‑94 (MQ=60)

                                                                                       CCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTCGCGTTT        >  SRR4302534.2116142/1‑94 (MQ=60)

                                                                                       CCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTCGCGTTT        >  SRR4302534.6315519/1‑94 (MQ=60)

                                                                                       CCAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTCGCGTTT        >  SRR4302534.1650882/1‑94 (MQ=60)

                                                                                        CAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTCGCGTTTA       <  SRR4302534.224763/94‑1 (MQ=60)

                                                                                        CAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTCGCGTTTA       <  SRR4302534.4464187/94‑1 (MQ=60)

                                                                                        CAGCACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTCGCGTTTA       >  SRR4302534.665717/1‑94 (MQ=60)

                                                                                           CACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTCGCGTTTATCG    >  SRR4302534.3242870/1‑94 (MQ=60)

                                                                                           CACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTCGCGTTTATCG    >  SRR4302534.3294309/1‑94 (MQ=60)

                                                                                            ACCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTCGCGTTTATCGT   >  SRR4302534.2303474/1‑94 (MQ=60)

                                                                                             CCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTCGCGTTTATCGTT  <  SRR4302534.3731237/94‑1 (MQ=60)

                                                                                             CCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTCGCGTTTATCGTT  <  SRR4302534.3836591/94‑1 (MQ=60)

TCAGCAACTGCTCTGCGCCAAACATCTCCGGCACTTCGGTCAGTACGGTAGTACCGCCGTTAGCAATCACGTAGTCAGAGAAACGCCCCAGCATCGGGTTAGCAGTAATACCAGAAAGACCGTCAGAACCACCGCACTCCAGACCAAACTTCAGTTCGCTGAGTTTGCCTGGCTCGCGTTTATCGTT  >  NC_000913/3240030‑3240216

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`