Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 884 257.2 12958910 96.9% 12557183 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,253,742 Δ1 bp coding (551/552 nt) yhaNM ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,253,7410G.99.6% 1043.5 / ‑3.5 224coding (552/552 nt)yhaNMhypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base . (129/94);  total (130/94)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.22e-01

Only 100 of 241 total aligned reads displayed.

ATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGA  >  NC_000913/3253649‑3253829
                                                                                            |                                                                                        
aTAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCg                                                                                          >  1:1070750/1‑93 (MQ=255)
aTAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCg                                                                                          >  1:4976572/1‑93 (MQ=255)
aTAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGACAGCGACg                                                                                          >  1:2250352/1‑93 (MQ=255)
 tAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCgt                                                                                         >  1:1923874/1‑92 (MQ=255)
   aCGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCgttc                                                                                       <  2:5337389/93‑4 (MQ=255)
   aCGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCgttc                                                                                       >  2:5001882/1‑90 (MQ=255)
   aCGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCgttc                                                                                       <  2:2210637/93‑4 (MQ=255)
   aCGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCgttc                                                                                       <  1:5855713/93‑4 (MQ=255)
    cGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCgttct                                                                                      <  2:3385762/93‑5 (MQ=255)
       acaATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGa                                                                                  >  2:799991/1‑93 (MQ=255)
        caATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGaa                                                                                 <  1:1208011/93‑1 (MQ=255)
         aaTTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGaaa                                                                                >  1:457546/1‑93 (MQ=255)
          aTTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAg                                                                               <  1:987306/93‑1 (MQ=255)
          aTTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAg                                                                               <  2:3074826/93‑1 (MQ=255)
          aTTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTAACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAg                                                                               >  1:147296/1‑93 (MQ=255)
             tAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCAc                                                                            >  2:666476/1‑93 (MQ=255)
             tAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCAc                                                                            <  2:5676557/93‑1 (MQ=255)
               gCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGGAAGCACCg                                                                          >  1:1368304/1‑93 (MQ=255)
               gCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCg                                                                          >  2:6311018/1‑93 (MQ=255)
               gCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCg                                                                          >  2:4548392/1‑93 (MQ=255)
                  tCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTACATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCa                                                                       >  1:6274325/1‑93 (MQ=255)
                   cgcgGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAg                                                                      >  2:3655008/1‑93 (MQ=255)
                    gcgGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCATCCAGCGTC‑GTTCTGGAAAGCACCGTCAGc                                                                     <  1:2866233/93‑1 (MQ=255)
                    gcgGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGc                                                                     >  1:4347937/1‑93 (MQ=255)
                    gcgGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGc                                                                     <  2:4215042/93‑1 (MQ=255)
                     cgGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCg                                                                    >  2:1636903/1‑93 (MQ=255)
                      ggAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCgg                                                                   >  2:5625817/1‑93 (MQ=255)
                       gAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGga                                                                  >  1:5788114/1‑93 (MQ=255)
                       gAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGga                                                                  >  1:2593276/1‑93 (MQ=255)
                         aTCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGgaga                                                                >  1:3372048/1‑93 (MQ=255)
                         aTCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGgaga                                                                <  1:5027229/93‑1 (MQ=255)
                          acGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGAc                                                               <  2:2171310/92‑1 (MQ=255)
                            gagaATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGActc                                                             <  1:3034369/93‑1 (MQ=255)
                             agaATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGActct                                                            <  2:1139329/93‑1 (MQ=255)
                             agaATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGActct                                                            >  1:2444757/1‑93 (MQ=255)
                             agaATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGActct                                                            <  2:5071004/93‑1 (MQ=255)
                              gaATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCtt                                                           >  2:3565491/1‑93 (MQ=255)
                               aaTAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTg                                                          <  2:6156866/93‑1 (MQ=255)
                                aTAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGc                                                         <  2:5834875/93‑1 (MQ=255)
                                  aaaGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTc                                                       >  2:2569239/1‑93 (MQ=255)
                                  aaaGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTc                                                       <  1:5704272/93‑1 (MQ=255)
                                  aaaGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTc                                                       <  1:5325557/93‑1 (MQ=255)
                                   aaGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCg                                                      >  1:913234/1‑93 (MQ=255)
                                   aaGCGGATCGCCGCAAACGGGACTTCATTGACGAACCTCAGGATCTCAGCCAGCGTC‑GTTCTTGAAAGCACCGTCAGCGGAGACTCTTGCTCg                                                      >  2:3047889/1‑93 (MQ=255)
                                    aGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGc                                                     >  2:4615797/1‑93 (MQ=255)
                                    aGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGc                                                     >  2:5641121/1‑93 (MQ=255)
                                     gCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGG‑TCTGGAAAGCACCGTCAGCGGAGACTCTTGGTCGcc                                                    >  2:1380003/1‑93 (MQ=255)
                                     gCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGcc                                                    >  1:4118522/1‑93 (MQ=255)
                                     gCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGcc                                                    >  2:6435097/1‑93 (MQ=255)
                                       ggATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGcccc                                                  >  1:1142088/1‑92 (MQ=255)
                                       ggATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCct                                                  >  2:5526080/1‑93 (MQ=255)
                                       ggATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCct                                                  >  2:1004550/1‑93 (MQ=255)
                                         aTCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCctct                                                >  2:1605669/1‑93 (MQ=255)
                                          tCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTg                                               >  1:6458633/1‑93 (MQ=255)
                                          tCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTg                                               >  2:5241904/1‑93 (MQ=255)
                                             ccgcAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCca                                            >  1:1041660/1‑93 (MQ=255)
                                              cgcAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCcac                                           >  1:3700592/1‑93 (MQ=255)
                                               gcAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGAACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCcaca                                          >  1:2033608/1‑93 (MQ=255)
                                                  aaCGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑TTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCacacac                                       >  2:2843958/1‑93 (MQ=255)
                                                   aCGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACg                                      >  2:4117952/1‑93 (MQ=255)
                                                    cGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGc                                     <  2:5577897/93‑1 (MQ=255)
                                                     gggACTTCATTGACGAACTTCAGGATCTCAGCCCGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCCCACAcccc                                    >  1:2958804/1‑93 (MQ=255)
                                                     gggACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGcc                                    <  2:2015507/93‑1 (MQ=255)
                                                      ggACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCct                                   <  2:5195460/93‑1 (MQ=255)
                                                      ggACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTAGCCCTCTGCCACACACGCct                                   >  1:552437/1‑93 (MQ=255)
                                                       gACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCctg                                  >  1:3246419/1‑93 (MQ=255)
                                                        aCTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCctgc                                 <  1:3640597/93‑1 (MQ=255)
                                                         cTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCctgct                                <  2:5736857/93‑1 (MQ=255)
                                                         cTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCctgct                                <  2:4162802/93‑1 (MQ=255)
                                                          ttCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCctgctg                               >  2:598021/1‑93 (MQ=255)
                                                           tCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTgg                              >  1:1421748/1‑93 (MQ=255)
                                                            cATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTggg                             <  2:2479779/93‑1 (MQ=255)
                                                                gACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTaac                         >  1:2085131/1‑92 (MQ=255)
                                                                gACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTaaa                         >  1:3129845/1‑93 (MQ=255)
                                                                gACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCAACGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTaaa                         >  1:4218453/1‑93 (MQ=255)
                                                                   gAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAacac                      >  2:2744310/1‑93 (MQ=255)
                                                                   gAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAacac                      <  1:2225195/93‑1 (MQ=255)
                                                                     aCTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAacacca                    >  1:3286850/1‑93 (MQ=255)
                                                                      cTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAacaccac                   >  2:3892047/1‑93 (MQ=255)
                                                                      cTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAacaccac                   >  1:3547276/1‑93 (MQ=255)
                                                                       ttCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAacaccaca                  >  1:1179473/1‑93 (MQ=255)
                                                                           ggATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCAt              <  1:809972/93‑1 (MQ=255)
                                                                            gATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATc             <  2:1503969/93‑1 (MQ=255)
                                                                             aTCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCg            >  1:6062995/1‑93 (MQ=255)
                                                                              tctcAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCgg           >  1:2674772/1‑92 (MQ=255)
                                                                              tctcAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGt           <  1:5141980/93‑1 (MQ=255)
                                                                              tctcAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCATCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGt           <  1:5004837/93‑1 (MQ=255)
                                                                               ctcAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTg          <  2:4699624/93‑1 (MQ=255)
                                                                               ctcAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTg          <  1:3957659/93‑1 (MQ=255)
                                                                                tcAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGc         <  2:3475459/93‑1 (MQ=255)
                                                                                tcAGCCAGCGTC‑GCTCTAGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCATACGATCGTGc         >  1:4430352/1‑93 (MQ=255)
                                                                                 cagccagcGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCg        <  1:4141159/93‑1 (MQ=255)
                                                                                  agccagcGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGt       >  2:4335904/1‑93 (MQ=255)
                                                                                  agccagcGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGt       >  2:2098600/1‑93 (MQ=255)
                                                                                  agccagcGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGt       <  1:254509/93‑1 (MQ=255)
                                                                                  agccagcGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGt       >  1:4017548/1‑93 (MQ=255)
                                                                                   gccagcGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGtc      >  2:2390015/1‑93 (MQ=255)
                                                                                   gccagcGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGtc      >  1:1654076/1‑93 (MQ=255)
                                                                                       gcGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGa  >  2:438278/1‑93 (MQ=255)
                                                                                       gcGTC‑GTTCTGCAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGa  <  2:3950757/93‑1 (MQ=255)
                                                                                            |                                                                                        
ATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGA  >  NC_000913/3253649‑3253829

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCA  >  NC_000913/3253649‑3253835
                                                                                             |                                                                                             
ATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGT                                                                                               >  SRR4302534.1974164/1‑94 (MQ=60)
   ACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCT                                                                                           <  SRR4302534.6014696/94‑1 (MQ=60)
        CAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAA                                                                                      >  SRR4302534.469111/1‑94 (MQ=60)
        CAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAA                                                                                      <  SRR4302534.1239799/94‑1 (MQ=60)
         AATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAG                                                                                     >  SRR4302534.409004/1‑94 (MQ=60)
         AATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTAACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAG                                                                                     >  SRR4302534.151136/1‑94 (MQ=60)
          ATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGC                                                                                    >  SRR4302534.1524437/1‑94 (MQ=60)
          ATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGC                                                                                    <  SRR4302534.1012550/94‑1 (MQ=60)
             TAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACC                                                                                 >  SRR4302534.5136682/1‑94 (MQ=60)
             TAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACC                                                                                 >  SRR4302534.1014955/1‑94 (MQ=60)
              AGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGGAAGCACCG                                                                                >  SRR4302534.1404350/1‑94 (MQ=60)
               GCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGGACCGT                                                                               >  SRR4302534.1364070/1‑94 (MQ=60)
               GCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGT                                                                               <  SRR4302534.3762124/94‑1 (MQ=60)
                CTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTC                                                                              >  SRR4302534.3777174/1‑94 (MQ=60)
                 TTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTACATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCA                                                                             >  SRR4302534.6444175/1‑94 (MQ=60)
                 TCCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCA                                                                             <  SRR4302534.5852725/94‑1 (MQ=60)
                  TCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAG                                                                            >  SRR4302534.5573339/1‑94 (MQ=60)
                  TCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAG                                                                            >  SRR4302534.1999844/1‑94 (MQ=60)
                  TCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAG                                                                            <  SRR4302534.916689/94‑1 (MQ=60)
                   CGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGC                                                                           >  SRR4302534.4465332/1‑94 (MQ=60)
                   CGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGC                                                                           >  SRR4302534.5349688/1‑94 (MQ=60)
                    GCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCATCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCG                                                                          <  SRR4302534.2941512/94‑1 (MQ=60)
                    GCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCG                                                                          >  SRR4302534.2013913/1‑94 (MQ=60)
                    GCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCG                                                                          <  SRR4302534.2941511/94‑1 (MQ=60)
                     CGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGG                                                                         >  SRR4302534.4363982/1‑94 (MQ=60)
                     CGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGG                                                                         >  SRR4302534.4682602/1‑94 (MQ=60)
                     CGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGG                                                                         >  SRR4302534.3071271/1‑94 (MQ=60)
                     CGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGG                                                                         >  SRR4302534.6508909/1‑94 (MQ=60)
                     CGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGG                                                                         <  SRR4302534.2694480/94‑1 (MQ=60)
                      GGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGA                                                                        >  SRR4302534.5945126/1‑94 (MQ=60)
                      GGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGA                                                                        >  SRR4302534.2661914/1‑94 (MQ=60)
                       GACTCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAG                                                                       <  SRR4302534.4255446/94‑1 (MQ=60)
                        AATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGA                                                                      >  SRR4302534.3462400/1‑94 (MQ=60)
                        AATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGA                                                                      >  SRR4302534.2165883/1‑94 (MQ=60)
                         ATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGAC                                                                     <  SRR4302534.5163014/94‑1 (MQ=60)
                          TCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATCGACGACCTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCCCCGTCAGCGGAGACT                                                                    <  SRR4302534.776194/94‑1 (MQ=60)
                           CGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTC                                                                   <  SRR4302534.5883297/94‑1 (MQ=60)
                            GAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCT                                                                  <  SRR4302534.1480553/94‑1 (MQ=60)
                            GAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCT                                                                  <  SRR4302534.3113940/94‑1 (MQ=60)
                            GAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCT                                                                  >  SRR4302534.2509731/1‑94 (MQ=60)
                            GAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCT                                                                  >  SRR4302534.1172565/1‑94 (MQ=60)
                             AGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTT                                                                 <  SRR4302534.1658493/94‑1 (MQ=60)
                             AGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTT                                                                 >  SRR4302534.3291472/1‑94 (MQ=60)
                             AGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTT                                                                 >  SRR4302534.2290833/1‑94 (MQ=60)
                              GAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTG                                                                <  SRR4302534.932771/94‑1 (MQ=60)
                              GAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTG                                                                >  SRR4302534.3610379/1‑94 (MQ=60)
                              GAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTG                                                                <  SRR4302534.4245059/94‑1 (MQ=60)
                               AATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGC                                                               >  SRR4302534.2661707/1‑94 (MQ=60)
                                 TAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTC                                                             >  SRR4302534.4214642/1‑94 (MQ=60)
                                  AAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCG                                                            >  SRR4302534.936459/1‑94 (MQ=60)
                                  AAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCG                                                            <  SRR4302534.5991814/94‑1 (MQ=60)
                                  AAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCG                                                            <  SRR4302534.5469202/94‑1 (MQ=60)
                                  AAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCG                                                            <  SRR4302534.5858790/94‑1 (MQ=60)
                                   AAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGC                                                           >  SRR4302534.3873697/1‑94 (MQ=60)
                                   AAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGC                                                           >  SRR4302534.3313023/1‑94 (MQ=60)
                                    AGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCC                                                          >  SRR4302534.4229338/1‑94 (MQ=60)
                                      CGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGGAAGCACCGTCAGCGGAGACTCTTGCTCGCCCC                                                        >  SRR4302534.2286834/1‑94 (MQ=60)
                                      CGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCC                                                        >  SRR4302534.1171987/1‑94 (MQ=60)
                                         ATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTG                                                     >  SRR4302534.5464592/1‑94 (MQ=60)
                                         ATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTG                                                     >  SRR4302534.6632617/1‑94 (MQ=60)
                                           CGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCC                                                   >  SRR4302534.3419115/1‑94 (MQ=60)
                                            GCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCA                                                  >  SRR4302534.211647/1‑94 (MQ=60)
                                            GCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCA                                                  >  SRR4302534.1068561/1‑94 (MQ=60)
                                             CCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCAC                                                 >  SRR4302534.3800069/1‑94 (MQ=60)
                                              CGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGAACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACA                                                >  SRR4302534.2086787/1‑94 (MQ=60)
                                               GCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACAC                                               >  SRR4302534.422247/1‑94 (MQ=60)
                                               GCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACAC                                               >  SRR4302534.2999900/1‑94 (MQ=60)
                                                  AACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACG                                            <  SRR4302534.6129706/94‑1 (MQ=60)
                                                    CGGGACTTCATTGACGAACTTCAGGATCTCAGCCCGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCCCACACCCC                                          >  SRR4302534.3036341/1‑94 (MQ=60)
                                                     GGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCT                                         <  SRR4302534.1218601/94‑1 (MQ=60)
                                                     GGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCT                                         <  SRR4302534.2565750/94‑1 (MQ=60)
                                                     GGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTAGCCCTCTGCCACACACGCCT                                         >  SRR4302534.566055/1‑94 (MQ=60)
                                                      GGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTG                                        <  SRR4302534.3341078/94‑1 (MQ=60)
                                                      GGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTG                                        >  SRR4302534.3332853/1‑94 (MQ=60)
                                                      GGACTTCATGGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTG                                        >  SRR4302534.6475992/1‑94 (MQ=60)
                                                       GACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGC                                       <  SRR4302534.599171/94‑1 (MQ=60)
                                                        ACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCT                                      <  SRR4302534.3738441/94‑1 (MQ=60)
                                                        ACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCT                                      >  SRR4302534.1189137/1‑94 (MQ=60)
                                                         CTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTG                                     <  SRR4302534.240776/94‑1 (MQ=60)
                                                          TTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGG                                    >  SRR4302534.1459158/1‑94 (MQ=60)
                                                           TCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGG                                   >  SRR4302534.3205153/1‑94 (MQ=60)
                                                               TGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAC                               >  SRR4302534.2139766/1‑94 (MQ=60)
                                                               TGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAA                               >  SRR4302534.3212115/1‑94 (MQ=60)
                                                               TGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCAACGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAA                               >  SRR4302534.4332196/1‑94 (MQ=60)
                                                                TACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAAC                              <  SRR4302534.5161412/94‑1 (MQ=60)
                                                                 ACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACA                             <  SRR4302534.4316348/94‑1 (MQ=60)
                                                                 ACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACA                             <  SRR4302534.4578143/94‑1 (MQ=60)
                                                                   GAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACC                           >  SRR4302534.5266365/1‑94 (MQ=60)
                                                                   GAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACC                           >  SRR4302534.5129825/1‑94 (MQ=60)
                                                                   GAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACC                           <  SRR4302534.2284127/94‑1 (MQ=60)
                                                                    AACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCA                          >  SRR4302534.3374484/1‑94 (MQ=60)
                                                                     ACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCAC                         >  SRR4302534.3642678/1‑94 (MQ=60)
                                                                      CTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACA                        >  SRR4302534.1210474/1‑94 (MQ=60)
                                                                      CTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACA                        >  SRR4302534.2540255/1‑94 (MQ=60)
                                                                          AGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCAT                    <  SRR4302534.5723043/94‑1 (MQ=60)
                                                                           GTATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATC                   <  SRR4302534.1070389/94‑1 (MQ=60)
                                                                           GGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATC                   <  SRR4302534.830326/94‑1 (MQ=60)
                                                                            GATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCG                  >  SRR4302534.6227849/1‑94 (MQ=60)
                                                                            GATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCG                  >  SRR4302534.997229/1‑94 (MQ=60)
                                                                            GATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCG                  <  SRR4302534.1443555/94‑1 (MQ=60)
                                                                            GATCTCAACCAGCGTC‑GTTCTGGAAATCACCGTCAGCGGAGCCTCTTGCTCGCCCTCTGCCACACTCGCCTGCTGGATAAACACCACACCAACG                  <  SRR4302534.1123401/94‑1 (MQ=60)
                                                                             ATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGT                 <  SRR4302534.1760866/94‑1 (MQ=60)
                                                                             ATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGT                 >  SRR4302534.3650516/1‑94 (MQ=60)
                                                                             ATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGG                 >  SRR4302534.2745490/1‑94 (MQ=60)
                                                                              TCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTG                >  SRR4302534.3094788/1‑94 (MQ=60)
                                                                              TCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTG                <  SRR4302534.5280744/94‑1 (MQ=60)
                                                                              TCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCATCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTG                <  SRR4302534.5140131/94‑1 (MQ=60)
                                                                               CTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGC               <  SRR4302534.4063634/94‑1 (MQ=60)
                                                                               CTCAGCCAGCGTC‑GCTCTAGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCATACGATCGTGC               >  SRR4302534.4549976/1‑94 (MQ=60)
                                                                                 CAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGT             <  SRR4302534.4252914/94‑1 (MQ=60)
                                                                                 CAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGT             >  SRR4302534.4125249/1‑94 (MQ=60)
                                                                                 CAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGT             <  SRR4302534.4803948/94‑1 (MQ=60)
                                                                                 CAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGT             >  SRR4302534.3554206/1‑94 (MQ=60)
                                                                                  AGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTC            <  SRR4302534.261140/94‑1 (MQ=60)
                                                                                  AGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTC            >  SRR4302534.1697185/1‑94 (MQ=60)
                                                                                   GCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCT           >  SRR4302534.2765169/1‑94 (MQ=60)
                                                                                     CAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCG         <  SRR4302534.1335665/94‑1 (MQ=60)
                                                                                       GCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGAT       <  SRR4302534.4488267/94‑1 (MQ=60)
                                                                                       GCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGAT       <  SRR4302534.6263807/94‑1 (MQ=60)
                                                                                        CGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAAAACCACACCATCGTGCGTCTCGATA      >  SRR4302534.1465273/1‑94 (MQ=60)
                                                                                         GTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCAGACCATCGTGCGTCTCGATAT     >  SRR4302534.2938549/1‑94 (MQ=60)
                                                                                         GTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATAT     <  SRR4302534.1221990/94‑1 (MQ=60)
                                                                                            CGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGC   <  SRR4302534.5746402/94‑1 (MQ=60)
                                                                                             GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCA  <  SRR4302534.1687355/94‑1 (MQ=60)
                                                                                             GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGAA  >  SRR4302534.1425408/1‑94 (MQ=60)
                                                                                             |                                                                                             
ATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCA  >  NC_000913/3253649‑3253835

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑