Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	884	257.2	12958910	96.9%	12557183	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,256,893	+C	coding (405/423 nt)	yhaQ ←	putative L‑serine dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,256,891	1	.	C	99.6%	854.6 / ‑3.9	246	coding (407/423 nt)	yhaQ	putative L‑serine dehydratase
Reads supporting (aligned to +/- strand): ref base . (0/1); new base C (126/119); total (126/120)
Fisher's exact test for biased strand distribution p-value = 4.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

Only 100 of 265 total aligned reads displayed.

GTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGT‑CCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAA  >  NC_000913/3256799‑3256978

gTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGt                                                                                          <  2:4920533/93‑1 (MQ=255)

 tttCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGT‑c                                                                                        >  2:5116327/1‑93 (MQ=255)

 tttCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGT‑c                                                                                        <  2:1197041/93‑1 (MQ=255)

  ttCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGT‑cc                                                                                       <  1:4431873/93‑1 (MQ=255)

   tCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGT‑ccc                                                                                      <  2:1718611/93‑2 (MQ=255)

     gACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAACCCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGT‑ccctt                                                                                    <  1:4121320/93‑4 (MQ=255)

     gACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGT‑ccctt                                                                                    <  2:4070632/93‑4 (MQ=255)

      aCATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGT‑cccttc                                                                                   <  2:4753707/93‑5 (MQ=255)

        aTCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCa                                                                                  >  1:4389242/1‑93 (MQ=255)

        aTCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCa                                                                                  >  2:3482030/1‑93 (MQ=255)

        aTCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCa                                                                                  >  2:2600446/1‑93 (MQ=255)

         tCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAg                                                                                 <  2:2757935/93‑1 (MQ=255)

          cGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAgg                                                                                >  1:2362720/1‑93 (MQ=255)

              cGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGt                                                                            <  1:371767/93‑1 (MQ=255)

               gACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTg                                                                           >  1:2749114/1‑93 (MQ=255)

                aCAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGa                                                                          <  1:3779606/93‑1 (MQ=255)

                aCAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGa                                                                          >  2:622343/1‑93 (MQ=255)

                 cAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGAt                                                                         <  1:3061452/93‑1 (MQ=255)

                 cAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGAt                                                                         <  2:5655515/93‑1 (MQ=255)

                   ggCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATcc                                                                       <  1:5636512/93‑1 (MQ=255)

                      cGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCa                                                                    <  2:1501582/93‑1 (MQ=255)

                      cGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCa                                                                    >  1:220921/1‑93 (MQ=255)

                       gAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCAt                                                                   <  1:626827/93‑1 (MQ=255)

                        aaGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCAtt                                                                  >  1:4682058/1‑93 (MQ=255)

                          gtgtTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTcc                                                                >  2:869967/1‑93 (MQ=255)

                          gtgtTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTcc                                                                >  1:5223679/1‑93 (MQ=255)

                            gtTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGt                                                              <  2:3047114/93‑1 (MQ=255)

                            gtTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGt                                                              >  2:699204/1‑93 (MQ=255)

                             ttcttcttcGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGtt                                                             <  2:4508504/93‑1 (MQ=255)

                             ttcttcttcGACAATAAACCCGCCGCAGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGtt                                                             <  1:5951659/93‑1 (MQ=255)

                              tcttcttcGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTtc                                                            <  1:2681127/93‑1 (MQ=255)

                               cttcttcGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTtct                                                           >  2:3198266/1‑93 (MQ=255)

                                 tcttcGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCa                                                         <  1:4836942/93‑1 (MQ=255)

                                 tcttcGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCa                                                         <  1:6172694/93‑1 (MQ=255)

                                  cttcGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCAt                                                        <  1:6135883/93‑1 (MQ=255)

                                  cttcGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCAt                                                        <  2:5752472/93‑1 (MQ=255)

                                   ttcGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATg                                                       >  2:2542815/1‑93 (MQ=255)

                                    tcGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATgg                                                      >  1:2470544/1‑93 (MQ=255)

                                    tcGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATgg                                                      <  1:5731653/93‑1 (MQ=255)

                                       aCAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGa                                                   >  2:1575291/1‑93 (MQ=255)

                                       aCAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTGCCAGGCAGTGATCCGCATGCCGTTCTCATGGCGa                                                   <  1:4320202/93‑1 (MQ=255)

                                         aaTAACCCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAgg                                                 <  1:6032839/93‑1 (MQ=255)

                                         aaTAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAgg                                                 >  1:2563604/1‑93 (MQ=255)

                                         aaTAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAgg                                                 >  2:5474466/1‑93 (MQ=255)

                                         aaTAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAgg                                                 >  1:1424717/1‑93 (MQ=255)

                                          aTAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGc                                                >  2:2650135/1‑93 (MQ=255)

                                          aTAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGc                                                <  2:3423139/93‑1 (MQ=255)

                                           tAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCa                                               >  1:51183/1‑93 (MQ=255)

                                             accccGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAAc                                             <  1:5904992/91‑1 (MQ=255)

                                             aaCCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAAc                                             >  2:3182184/1‑93 (MQ=255)

                                                ccgccgccgACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACAtt                                          <  2:5920582/93‑1 (MQ=255)

                                                ccgccgccgACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACAtt                                          >  2:6064119/1‑93 (MQ=255)

                                                ccgccgccgACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATACGCATTCCGCTCTCATGGCGAGGGCACAtt                                          >  2:249964/1‑93 (MQ=255)

                                                 cgccgccgACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACAttt                                         >  1:4292470/1‑93 (MQ=255)

                                                 cgccgccgACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACAttt                                         >  2:639679/1‑93 (MQ=255)

                                                  gccgccgACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTc                                        >  2:4386238/1‑93 (MQ=255)

                                                   ccgccgACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCg                                       <  1:4990200/93‑1 (MQ=255)

                                                     gccgACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCggg                                     <  2:3345507/93‑1 (MQ=255)

                                                     gccgACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCggg                                     <  1:4519188/93‑1 (MQ=255)

                                                      ccgACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGa                                    <  2:3451468/93‑1 (MQ=255)

                                                       cgACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATTGCGAGGCAACATTTCGGGAt                                   <  1:3650948/93‑1 (MQ=255)

                                                       cgACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGAt                                   <  2:5019809/93‑1 (MQ=255)

                                                        gACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATg                                  >  1:96358/1‑93 (MQ=255)

                                                          cAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGa                                >  1:2076342/1‑93 (MQ=255)

                                                           agagTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGaa                               <  1:5501228/93‑1 (MQ=255)

                                                           agagTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGaa                               >  1:1846804/1‑93 (MQ=255)

                                                            gagTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAg                              >  1:1062707/1‑93 (MQ=255)

                                                              gTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGAt                            <  2:5811300/93‑1 (MQ=255)

                                                                aaTAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATaa                          >  1:139734/1‑93 (MQ=255)

                                                                 aTAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAAt                         >  1:4527381/1‑93 (MQ=255)

                                                                  tAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATg                        >  2:3089962/1‑93 (MQ=255)

                                                                   aGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGt                       <  2:207718/93‑1 (MQ=255)

                                                                    ggTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGtt                      >  1:579087/1‑93 (MQ=255)

                                                                      ttttACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCt                    <  1:706760/93‑1 (MQ=255)

                                                                       tttACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGAATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCtt                   <  1:3317062/93‑1 (MQ=255)

                                                                        ttACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCttt                  <  2:4008369/93‑1 (MQ=255)

                                                                         tACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTg                 >  2:1041681/1‑93 (MQ=255)

                                                                          aCTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGc                <  1:841498/93‑1 (MQ=255)

                                                                          aCTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGc                <  2:4615827/93‑1 (MQ=255)

                                                                          aCTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGc                <  1:1186198/93‑1 (MQ=255)

                                                                           cTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCt               <  1:5348306/93‑1 (MQ=255)

                                                                            ttAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTa              <  2:1288421/93‑1 (MQ=255)

                                                                              aaTAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACa            <  1:3207210/93‑1 (MQ=255)

                                                                               aTAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAg           <  2:2395309/93‑1 (MQ=255)

                                                                               aTAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAg           >  2:5391529/1‑93 (MQ=255)

                                                                               aTAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGGTCTCATGGCGAGGCAACATTTCGGGCTGGAAGATAATGTTCTTTGCTACAg           >  1:5686346/1‑93 (MQ=255)

                                                                                tAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAgg          <  2:4454382/93‑1 (MQ=255)

                                                                                 aGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGa         >  2:381791/1‑93 (MQ=255)

                                                                                 aGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGa         >  1:2085080/1‑93 (MQ=255)

                                                                                  gCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGaa        <  2:5986459/93‑1 (MQ=255)

                                                                                  gCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGaa        <  2:4333307/93‑1 (MQ=255)

                                                                                   ctctTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGaaa       >  1:3864290/1‑93 (MQ=255)

                                                                                   ctctTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGaaa       >  1:2623993/1‑93 (MQ=255)

                                                                                    tctTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGaaaa      >  2:2939277/1‑93 (MQ=255)

                                                                                    tctTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGaaaa      >  1:93934/1‑93 (MQ=255)

                                                                                    tctTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGaaaa      >  2:1872638/1‑93 (MQ=255)

                                                                                     ctTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATNATGTTCTTTGCTACANGAANAt     >  2:5234631/1‑93 (MQ=255)

                                                                                      ttCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATc    <  2:2218761/93‑1 (MQ=255)

                                                                                        ccTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATcaa  >  2:4871768/1‑93 (MQ=255)

                                                                                        ccTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATcaa  <  2:2035249/93‑1 (MQ=255)

GTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGT‑CCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAA  >  NC_000913/3256799‑3256978

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGT‑C‑CTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATG  >  NC_000913/3256800‑3256985

TTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGT‑C‑C                                                                                              <  SRR4302534.5811435/94‑1 (MQ=60)

 TTCTACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGT‑C‑CC                                                                                             <  SRR4302534.5994551/94‑1 (MQ=60)

 TTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGT‑C‑CC                                                                                             <  SRR4302534.4551540/94‑1 (MQ=60)

  TCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGT‑C‑CCT                                                                                            >  SRR4302534.6503318/1‑94 (MQ=60)

   CGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGT‑C‑Cctt                                                                                           <  SRR4302534.3817562/94‑4 (MQ=60)

    GACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAACCCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCC‑CTTC                                                                                           <  SRR4302534.4232232/94‑1 (MQ=60)

    GACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCC‑CTTC                                                                                           >  SRR4302534.2638057/1‑94 (MQ=60)

     ACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCC‑CTTCC                                                                                          <  SRR4302534.2718658/94‑1 (MQ=60)

      CATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCC‑CTTCCA                                                                                         >  SRR4302534.4507597/1‑94 (MQ=60)

      CATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCC‑CTTCCA                                                                                         <  SRR4302534.4114932/94‑1 (MQ=60)

      CATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCC‑CTTCCA                                                                                         <  SRR4302534.2263415/94‑1 (MQ=60)

        TCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCC‑CTTCCAGG                                                                                       >  SRR4302534.2425436/1‑94 (MQ=60)

           TGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCC‑CTTCCAGGCAG                                                                                    >  SRR4302534.4769774/1‑94 (MQ=60)

            GCGACAGGCCGAAGTGTTCTTCTTCGACAATAACCCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCC‑CTTCCAGGCAGT                                                                                   <  SRR4302534.1003322/94‑1 (MQ=60)

            GCGACAGGCCGAAGTGTTCATCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCC‑CTTCCAGGCAGT                                                                                   <  SRR4302534.6108695/94‑1 (MQ=60)

             CGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCC‑CTTCCAGGCAGTG                                                                                  >  SRR4302534.2821537/1‑94 (MQ=60)

             CGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCC‑CTTCCAGGCAGTG                                                                                  <  SRR4302534.381255/94‑1 (MQ=60)

              GACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCC‑CTTCCAGGCAGTGA                                                                                 <  SRR4302534.4090153/94‑1 (MQ=60)

               ACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCC‑CTTCCAGGCAGTGAT                                                                                <  SRR4302534.3881082/94‑1 (MQ=60)

                CAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCC‑CTTCCAGGCAGTGATC                                                                               <  SRR4302534.3141799/94‑1 (MQ=60)

                CAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCC‑CTTCCAGGCAGTGATC                                                                               >  SRR4302534.18937/1‑94 (MQ=60)

                CAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCC‑CTTCCAGGCAGTGATC                                                                               <  SRR4302534.2148094/94‑1 (MQ=60)

                  GGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCC‑CTTCCAGGCAGTGATCCG                                                                             <  SRR4302534.5789078/94‑1 (MQ=60)

                    CCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCC‑CTTCCAGGCAGTGATCCGCA                                                                           >  SRR4302534.226714/1‑94 (MQ=60)

                     CGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCC‑CTTCCAGGCAGTGATCCGCAT                                                                          >  SRR4302534.3925487/1‑94 (MQ=60)

                     AGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGCAATAGGTTTTACTTAATAGCTCTTCCTGTCC‑CTTCCAGGCAGTGGTGCGCAT                                                                          >  SRR4302534.6149811/1‑94 (MQ=60)

                      GAAGTGTTCTTCTTCGACAATAACCCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCC‑CTTCCAGGCAGTGATCCGCATT                                                                         <  SRR4302534.3419271/94‑1 (MQ=60)

                      GAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCC‑CTTCCAGGCAGTGATCCGCATT                                                                         >  SRR4302534.3454322/1‑94 (MQ=60)

                      GAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCC‑CTTCCAGGCAGTGATCCGCATT                                                                         >  SRR4302534.3308253/1‑94 (MQ=60)

                      GAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCC‑CTTCCAGGCAGTGATCCGCATT                                                                         >  SRR4302534.4809171/1‑94 (MQ=60)

                      GAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCC‑CTTCCAGGCAGTGATCCGCATT                                                                         <  SRR4302534.642025/94‑1 (MQ=60)

                        AGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCC‑CTTCCAGGCAGTGATCCGCATTCC                                                                       >  SRR4302534.967701/1‑94 (MQ=60)

                        AGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCC‑CTTCCAGGCAGTGATCCGCATTCC                                                                       <  SRR4302534.2447557/94‑1 (MQ=60)

                        AGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCC‑CTTCCAGGCAGTGATCCGCATTCC                                                                       >  SRR4302534.4402696/1‑94 (MQ=60)

                        AGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCC‑CTTCCAGGCAGTGATCCGCATTCC                                                                       >  SRR4302534.5364687/1‑94 (MQ=60)

                         GTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCC‑CTTCCAGGCAGTGATCCGCATTCCG                                                                      <  SRR4302534.4511739/94‑1 (MQ=60)

                         GTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCC‑CTTCCAGGCAGTGATCCGCATTCCG                                                                      <  SRR4302534.5398321/94‑1 (MQ=60)

                         GTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCC‑CTTCCAGGCAGTGATCCGCATTCCG                                                                      <  SRR4302534.3109410/94‑1 (MQ=60)

                         GTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCC‑CTTCCAGGCAGTGATCCGCATTCCG                                                                      >  SRR4302534.4650940/1‑94 (MQ=60)

                            TTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCC‑CTTCCAGGCAGTGATCCGCATTCCGTTC                                                                   <  SRR4302534.4236569/94‑1 (MQ=60)

                            TTCTTCTTCGACAATAAACCCGCCGCAGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCC‑CTTCCAGGCAGTGATCCGCATTCCGTTC                                                                   <  SRR4302534.6113260/94‑1 (MQ=60)

                             TCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCC‑CTTCCAGGCAGTGATCCGCATTCCGTTCT                                                                  <  SRR4302534.2752000/94‑1 (MQ=60)

                                TCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCC‑CTTCCAGGCAGTGATCCGCATTCCGTTCTCAT                                                               <  SRR4302534.2285731/94‑1 (MQ=60)

                                TCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCC‑CTTCCAGGCAGTGATCCGCATTCCGTTCTCAT                                                               <  SRR4302534.6340612/94‑1 (MQ=60)

                                TCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCC‑CTTCCAGGCAGTGATCCGCATTCCGTTCTCAT                                                               <  SRR4302534.4968159/94‑1 (MQ=60)

                                TCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCC‑CTTCCAGGCAGTGATCCGCATTCCGTTCTCAT                                                               >  SRR4302534.2555693/1‑94 (MQ=60)

                                 CTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCC‑CTTCCAGGCAGTGATCCGCATTCCGTTCTCATG                                                              >  SRR4302534.3874537/1‑94 (MQ=60)

                                 CTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCC‑CTTCCAGGCAGTGATCCGCATTCCGTTCTCATG                                                              <  SRR4302534.6302781/94‑1 (MQ=60)

                                  TTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCC‑CTTCCAGGCAGTGATCCGCATTCCGTTCTCATGG                                                             >  SRR4302534.2536209/1‑94 (MQ=60)

                                   TCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCC‑CTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGC                                                            <  SRR4302534.5886967/94‑1 (MQ=60)

                                    CGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCC‑CTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCG                                                           <  SRR4302534.6273831/94‑1 (MQ=60)

                                    CGAAAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCC‑CTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCG                                                           <  SRR4302534.3385629/94‑1 (MQ=60)

                                      ACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCC‑CTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAG                                                         <  SRR4302534.5659267/94‑1 (MQ=60)

                                      ACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCC‑CTGCCAGGCAGTGATCCGCATGCCGTTCTCATGGCGAG                                                         <  SRR4302534.4437145/94‑1 (MQ=60)

                                      ACAATAAACCCGCCGCCGACAGAGTAAGAGGTTTTACTTAATAGCTCTTCCTGTCC‑CTTCCAGGCAGTGATCCGCATTCCGTTCTCGTGGCGAG                                                         >  SRR4302534.2224206/1‑94 (MQ=60)

                                       CAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCC‑CTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGG                                                        >  SRR4302534.1462194/1‑94 (MQ=60)

                                       CAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCC‑CTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGG                                                        >  SRR4302534.2631547/1‑94 (MQ=60)

                                       CAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCC‑CTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGG                                                        >  SRR4302534.2794191/1‑94 (MQ=60)

                                        AATAACCCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCC‑CTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGC                                                       <  SRR4302534.6196791/94‑1 (MQ=60)

                                        AATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCC‑CTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGC                                                       >  SRR4302534.75647/1‑94 (MQ=60)

                                         ATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCC‑CTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCA                                                      >  SRR4302534.4891378/1‑94 (MQ=60)

                                         ATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCC‑CTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCA                                                      >  SRR4302534.52557/1‑94 (MQ=60)

                                         ATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCC‑CTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCA                                                      >  SRR4302534.4647943/1‑94 (MQ=60)

                                           AAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCC‑CTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAAC                                                    >  SRR4302534.3879544/1‑94 (MQ=60)

                                           AAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCC‑CTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAAC                                                    >  SRR4302534.2048108/1‑94 (MQ=60)

                                            AGCCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCC‑CTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACA                                                   <  SRR4302534.5794002/94‑1 (MQ=60)

                                            ACCCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCC‑CTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACA                                                   <  SRR4302534.6065337/94‑1 (MQ=60)

                                            AACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCC‑CTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACA                                                   <  SRR4302534.5377230/94‑1 (MQ=60)

                                               CCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCC‑CTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTT                                                <  SRR4302534.265239/94‑1 (MQ=60)

                                               CCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCC‑CTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTT                                                <  SRR4302534.907093/94‑1 (MQ=60)

                                               CCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCC‑CTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTT                                                >  SRR4302534.4408771/1‑94 (MQ=60)

                                                 GCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCC‑CTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCG                                              >  SRR4302534.5055987/1‑94 (MQ=60)

                                                  CCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCC‑CTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGG                                             <  SRR4302534.5125211/94‑1 (MQ=60)

                                                   CGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCC‑CTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGG                                            <  SRR4302534.3280486/94‑1 (MQ=60)

                                                    GCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCC‑CTTCCAGGCAGTGCTACGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGA                                           >  SRR4302534.6025206/1‑94 (MQ=60)

                                                    GCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCC‑CTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGA                                           <  SRR4302534.4641610/94‑1 (MQ=60)

                                                      CGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCC‑CTTCCAGGCAGTGATCCGCATTCCGTTCTCATTGCGAGGCAACATTTCGGGATG                                         <  SRR4302534.3749070/94‑1 (MQ=60)

                                                      CGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCC‑CTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATG                                         <  SRR4302534.5878736/94‑1 (MQ=60)

                                                      CGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCC‑CTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATG                                         >  SRR4302534.98934/1‑94 (MQ=60)

                                                      CGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCC‑CTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATG                                         <  SRR4302534.1241874/94‑1 (MQ=60)

                                                      CGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCC‑CTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATG                                         >  SRR4302534.6108804/1‑94 (MQ=60)

                                                        actcAGTAATAGGTTTTACTTAATAGCGCTTCCTGT‑CACTTTCAGGCAGGGATCCGCATGCCGTTCTCATGGCGAGGCAACATTGCGGGATGGA                                       <  SRR4302534.5280395/90‑1 (MQ=60)

                                                        ACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCC‑CTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGA                                       >  SRR4302534.2130701/1‑94 (MQ=60)

                                                         CAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCC‑CTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAA                                      <  SRR4302534.4499050/94‑1 (MQ=60)

                                                         CAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCC‑CTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAA                                      >  SRR4302534.1894850/1‑94 (MQ=60)

                                                         CAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCC‑CTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAA                                      <  SRR4302534.2779170/94‑1 (MQ=60)

                                                          AGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCC‑CTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAG                                     <  SRR4302534.5109925/94‑1 (MQ=60)

                                                          AGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCC‑CTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAG                                     <  SRR4302534.5649595/94‑1 (MQ=60)

                                                          AGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCC‑CTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAG                                     >  SRR4302534.1090252/1‑94 (MQ=60)

                                                            AGTAATAGGTTTTACTTAATAGCTCTTCCTGTCC‑CTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGAT                                   <  SRR4302534.6373206/94‑1 (MQ=60)

                                                            AGTAATAGGTTTTACTTAATAGCTCTTCCTGTCC‑CTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGAT                                   >  SRR4302534.262042/1‑94 (MQ=60)

                                                              TAATAGGTTTTACTTAATAGCTCTTCCTGTCC‑CTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAA                                 >  SRR4302534.143393/1‑94 (MQ=60)

                                                              TAATAGGTTTTACTTAATAGCTCTTCCTGTCC‑CTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAA                                 >  SRR4302534.5881998/1‑94 (MQ=60)

                                                               AATAGGTTTTACTTAATAGCTCTTCCTGTCC‑CTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAAT                                >  SRR4302534.4650030/1‑94 (MQ=60)

                                                               AATAGGTTTTACTTAATAGCTCTTCCTGTCC‑CTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAAT                                >  SRR4302534.5229214/1‑94 (MQ=60)

                                                                ATAGGTTTTATTTAATAGCTGTTCCTGTCC‑CTTCCAGGCAGTGATCCGCATTCGGTTCTCATGGCGAGGCAGCATTTCGGGAgggaattaaatt                               >  SRR4302534.1645319/1‑82 (MQ=60)

                                                                 TAGGTTTTACTTAATAGCTCTTCCTGTCC‑CTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGT                              >  SRR4302534.1450164/1‑94 (MQ=60)

                                                                  AGGTTTTACTTAATAGCTCTTCCTGTCC‑CTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTT                             <  SRR4302534.1154802/94‑1 (MQ=60)

                                                                  AGGTTTTACTTAATAGCTCTTCCTGTCC‑CTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTT                             >  SRR4302534.6334408/1‑94 (MQ=60)

                                                                  AGGTTTTACTTAATAGCTCTTCCTGTCC‑CTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTT                             >  SRR4302534.593279/1‑94 (MQ=60)

                                                                  AGGTTTTACTTAATAGCTCTTCCTGTCC‑CTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTT                             <  SRR4302534.5231755/94‑1 (MQ=60)

                                                                    GTTTTACTTAATAGCTCTTCCTGTCC‑CTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCT                           <  SRR4302534.2281814/94‑1 (MQ=60)

                                                                    GTTTTACTTAATAGCTCTTCCTGTCC‑CTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCT                           <  SRR4302534.4425764/94‑1 (MQ=60)

                                                                    GTTTTACTTAATAGCTCTTCCTGTCC‑CTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCT                           >  SRR4302534.1335886/1‑94 (MQ=60)

                                                                     TTTTACTTAATAGCTCTTCCTGTCC‑CTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTT                          <  SRR4302534.724107/94‑1 (MQ=60)

                                                                      TTTACTTAATAGCTCTTCCTGTCC‑CTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTT                         <  SRR4302534.1694539/94‑1 (MQ=60)

                                                                      TTTACTTAATAGCTCTTCCTGTCC‑CTTCCAGGCAGTGATCCGAATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTT                         <  SRR4302534.3405728/94‑1 (MQ=60)

                                                                       TTACTTAATAGCTCTTCCTGTCC‑CTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTG                        <  SRR4302534.5360015/94‑1 (MQ=60)

                                                                        TACTTAATAGCTCTTCCTGTCC‑CTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGC                       >  SRR4302534.3893961/1‑94 (MQ=60)

                                                                         ACTTAATAGCTCTTCCTGTCC‑CTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCT                      <  SRR4302534.1217385/94‑1 (MQ=60)

                                                                         ACTTAATAGCTCTTCCTGTCC‑CTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCT                      <  SRR4302534.862693/94‑1 (MQ=60)

                                                                          CTTAATAGCTCTTCCTGTCC‑CTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTA                     <  SRR4302534.5492513/94‑1 (MQ=60)

                                                                           TTAATAGCTCTTCCTGTCC‑CTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTAC                    <  SRR4302534.2940394/94‑1 (MQ=60)

                                                                           TTAATAGCTCTTCCTGTCC‑CTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTAC                    >  SRR4302534.976733/1‑94 (MQ=60)

                                                                           TTAATAGCTCTTCCTGTCC‑CTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTAC                    <  SRR4302534.590343/94‑1 (MQ=60)

                                                                            TAATAGCTCTTCCTGTCC‑CTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACA                   >  SRR4302534.4567157/1‑94 (MQ=60)

                                                                            TAATAGCTCTTCCTGTCC‑CTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACA                   >  SRR4302534.687078/1‑94 (MQ=60)

                                                                             AATAGCTCTTCCTGTCC‑CTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAG                  <  SRR4302534.3292262/94‑1 (MQ=60)

                                                                             AATAGCTCTTCCTGTCC‑CTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAG                  >  SRR4302534.6258689/1‑94 (MQ=60)

                                                                             AATAGCTCTTCCTGTCC‑CTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAG                  <  SRR4302534.4535693/94‑1 (MQ=60)

                                                                             AATAGCTCTTCCTGTCC‑CTTCCAGGCAGTGATCCGCATTCCGGTCTCATGGCGAGGCAACATTTCGGGCTGGAAGATAATGTTCTTTGCTACAG                  >  SRR4302534.5840336/1‑94 (MQ=60)

                                                                               TAGCTCTTCCTGTCC‑CTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGA                >  SRR4302534.2139713/1‑94 (MQ=60)

                                                                                AGCTCTTCCTGTCC‑CTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAA               >  SRR4302534.4646302/1‑94 (MQ=60)

                                                                                 GCTCTTCCTGTCC‑CTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAA              >  SRR4302534.3968046/1‑94 (MQ=60)

                                                                                 GCTCTTCCTGTCC‑CTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAA              <  SRR4302534.876073/94‑1 (MQ=60)

                                                                                 GCTCTTCCTGTCC‑CTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAA              >  SRR4302534.2693410/1‑94 (MQ=60)

                                                                                  CTCTTCCTGTCC‑CTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAA             >  SRR4302534.96444/1‑94 (MQ=60)

                                                                                    CTTCCTGTCC‑CTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATC           >  SRR4302534.5507548/1‑94 (MQ=60)

                                                                                    CTTCCTGTCC‑CTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATC           >  SRR4302534.6361267/1‑94 (MQ=60)

                                                                                     TTCCTGTCC‑CTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCA          >  SRR4302534.2136453/1‑94 (MQ=60)

                                                                                     TTCCTGTCC‑CTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCA          <  SRR4302534.1996769/94‑1 (MQ=60)

                                                                                        CTGTCC‑CTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACA       <  SRR4302534.4001974/94‑1 (MQ=60)

                                                                                        CTGTCC‑CTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACA       <  SRR4302534.2949860/94‑1 (MQ=60)

                                                                                         TGTCC‑CTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAA      <  SRR4302534.1834130/94‑1 (MQ=60)

                                                                                         TGTCC‑CTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAA      >  SRR4302534.4623846/1‑94 (MQ=60)

                                                                                         TGTCC‑CTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAA      >  SRR4302534.2137350/1‑94 (MQ=60)

                                                                                         TGTCC‑CTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAA      >  SRR4302534.2228840/1‑94 (MQ=60)

                                                                                         TGTCC‑CTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAA      >  SRR4302534.5949349/1‑94 (MQ=60)

                                                                                         TGTCC‑CTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAA      <  SRR4302534.2370751/94‑1 (MQ=60)

                                                                                         TGTCC‑CTTCCAGGCAGTGATCCGCACTCCGTTCTCATGGCGAGGCAACATTTCGGAATGGCAGATAATGTTTTTCTCTACAGGGAAATCAACAA      >  SRR4302534.5573294/1‑94 (MQ=60)

                                                                                          gtcC‑CTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAAT     >  SRR4302534.2092230/4‑94 (MQ=60)

                                                                                           tcC‑CTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATA    <  SRR4302534.16784/92‑1 (MQ=60)

                                                                                           tcC‑CTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATA    >  SRR4302534.5280326/3‑94 (MQ=60)

                                                                                           C‑C‑CTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATAT   >  SRR4302534.2060913/1‑94 (MQ=60)

                                                                                             C‑CTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATG  <  SRR4302534.4826431/94‑1 (MQ=60)

                                                                                             C‑CTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATG  <  SRR4302534.5018644/94‑1 (MQ=60)

                                                                                             C‑CTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATG  <  SRR4302534.196980/94‑1 (MQ=60)

TTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGT‑C‑CTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATG  >  NC_000913/3256800‑3256985

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`