Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A0 F0 I1 R1 245 579.6 28867798 97.7% 28203838 96.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,257,838 +G coding (2222/2241 nt) tdcE ← putative formate acetyltransferase 3

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,257,8371.G99.8% 2232.0 / ‑4.2 571coding (2223/2241 nt)tdcEputative formate acetyltransferase 3
Reads supporting (aligned to +/- strand):  ref base . (1/0);  new base G (289/281);  total (290/281)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.71e-01

Only 100 of 622 total aligned reads displayed.

ATCTTTTTCATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCAC‑GCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACG  >  NC_000913/3257742‑3257927
                                                                                                |                                                                                          
aTCTTTTTCATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCAc                                                                                             <  1:2561216/96‑1 (MQ=255)
aTCTTTTTCATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCAc                                                                                             <  1:7433203/96‑1 (MQ=255)
  cTTTTTCATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCAc‑gg                                                                                          <  1:6805007/96‑2 (MQ=255)
   tttttCATACATCCTCCGGGGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCAc‑ggt                                                                                         <  1:8860731/96‑3 (MQ=255)
    ttttCATACTTCATACTGCGTCTTCGCGCCTCGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGAGCAc‑ggcg                                                                                        <  1:14376093/96‑4 (MQ=255)
    ttttCATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCAc‑ggcg                                                                                        >  2:5109550/1‑93 (MQ=255)
       tCATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAg                                                                                      >  2:2172532/1‑96 (MQ=255)
          tACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCa                                                                                   <  1:2736468/96‑1 (MQ=255)
           aCATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCag                                                                                  >  2:3475086/1‑96 (MQ=255)
           aCATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCag                                                                                  >  1:1070571/1‑96 (MQ=255)
           aCATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCag                                                                                  >  1:6468366/1‑96 (MQ=255)
            cATCCTCCGGCGTCAGAGCGCCTGGGTAAATGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCaga                                                                                 <  2:9208843/96‑1 (MQ=255)
            cATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCaga                                                                                 <  2:2386595/96‑1 (MQ=255)
            cATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCaga                                                                                 >  2:57207/1‑96 (MQ=255)
              tcctccGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAgaga                                                                               <  2:1480281/96‑1 (MQ=255)
              tcctccGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAgaga                                                                               <  1:4622892/96‑1 (MQ=255)
              tcctccGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAgaga                                                                               <  2:4033981/96‑1 (MQ=255)
               cctccGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGac                                                                              <  1:1794298/96‑1 (MQ=255)
               cctccGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGac                                                                              >  1:10001865/1‑96 (MQ=255)
                ctccGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGaca                                                                             >  2:11768610/1‑96 (MQ=255)
                ctccGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGaca                                                                             >  1:3347307/1‑96 (MQ=255)
                   cGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACgg                                                                          <  1:8548424/96‑1 (MQ=255)
                    ggCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGa                                                                         >  1:7852189/1‑96 (MQ=255)
                    ggCGTCAGAGCGCCTGGGAAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGa                                                                         >  2:7603971/1‑96 (MQ=255)
                     gCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGAt                                                                        <  1:10475561/96‑1 (MQ=255)
                     gCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGAt                                                                        <  1:5729764/96‑1 (MQ=255)
                       gTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTg                                                                      >  1:441575/1‑96 (MQ=255)
                          agagCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCa                                                                   >  1:5520518/1‑96 (MQ=255)
                           gagaGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAg                                                                  <  1:13947966/96‑1 (MQ=255)
                            agCGCCTGGGTAAAGGTACGTGAAATACCATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAgg                                                                 <  2:8145302/96‑1 (MQ=255)
                             gcgcCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGt                                                                >  2:7201489/1‑96 (MQ=255)
                              cgcCTGGGTAAAGGTACGTGAAATAACCTCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGtt                                                               <  2:10666076/96‑1 (MQ=255)
                              cgcCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGtt                                                               <  2:13092244/96‑1 (MQ=255)
                              cgcCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGtt                                                               <  2:9482044/96‑1 (MQ=255)
                                cgtGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACTCGGATTGTCAGGTTAg                                                             >  1:12813267/3‑96 (MQ=255)
                                ccTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAg                                                             <  1:11543455/96‑1 (MQ=255)
                                 cTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTatg                                                            <  1:12078179/96‑3 (MQ=255)
                                 cTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAgg                                                            <  2:1403510/96‑1 (MQ=255)
                                   gggTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGAGTGTCAGGTTAGGat                                                          >  1:5328189/1‑96 (MQ=255)
                                   gggTAAAGGTACGTGAAATAACATCCTGTTGCCGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGat                                                          <  2:10981988/96‑1 (MQ=255)
                                    ggTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGata                                                         >  2:13785938/1‑96 (MQ=255)
                                     gTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGatat                                                        >  1:13536270/1‑96 (MQ=255)
                                        aaGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATAtttt                                                     >  1:63279/1‑96 (MQ=255)
                                        aaGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATAtttt                                                     >  2:6198868/1‑96 (MQ=255)
                                         aGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATAttttt                                                    <  1:3508246/96‑1 (MQ=255)
                                           gTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTcc                                                  >  1:8007706/1‑96 (MQ=255)
                                             aCGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCgg                                                >  2:12684714/1‑96 (MQ=255)
                                             aCGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCgg                                                <  1:13132534/96‑1 (MQ=255)
                                               gTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGt                                              <  1:3669167/96‑1 (MQ=255)
                                               gTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGGCAGGTTAGGATATTTTTCCGGGt                                              >  2:3801466/1‑96 (MQ=255)
                                                 gAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGc                                            <  1:392808/96‑1 (MQ=255)
                                                 gAAATAACATCCTGTTGCTGTTCACGGGTAAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGc                                            >  1:10940245/1‑96 (MQ=255)
                                                  aaaTAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCt                                           <  2:4672484/96‑1 (MQ=255)
                                                   aaTAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTc                                          <  2:9799711/96‑1 (MQ=255)
                                                   aaTAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTc                                          <  1:11812089/96‑1 (MQ=255)
                                                    aTAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGGTAGGATATTTTTCCGGGTGCTCg                                         >  2:30333/1‑96 (MQ=255)
                                                    aTAACATCCTGTTGCTGTTCACGGGTCAGTGCGGTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGGGCTCg                                         >  2:11597228/1‑96 (MQ=37)
                                                       aCATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATg                                      <  1:7736856/96‑1 (MQ=255)
                                                        cATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATgg                                     >  2:1747369/1‑96 (MQ=255)
                                                          tCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCa                                   >  1:3143309/1‑96 (MQ=255)
                                                           ccTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCAt                                  >  1:9285571/1‑96 (MQ=255)
                                                            cTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATc                                 <  1:7307263/96‑1 (MQ=255)
                                                              gTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCca                               >  2:10828990/1‑96 (MQ=255)
                                                              gTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCca                               <  1:11230649/96‑1 (MQ=255)
                                                              gTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCca                               <  1:9024619/96‑1 (MQ=255)
                                                               ttGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCcag                              >  2:4439077/1‑96 (MQ=255)
                                                                tGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCcagc                             >  2:11297162/1‑96 (MQ=255)
                                                                tGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCcagc                             <  2:2049027/96‑1 (MQ=255)
                                                                tGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCcacc                             >  2:5327621/1‑94 (MQ=255)
                                                                 gCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCcagca                            <  1:5110094/96‑1 (MQ=255)
                                                                   tGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCagcagc                          >  2:8600611/1‑96 (MQ=255)
                                                                    gTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAgcagca                         >  2:7943123/1‑96 (MQ=255)
                                                                     ttCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCAt                        >  1:9972630/1‑96 (MQ=255)
                                                                      tCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCAtt                       >  2:12220347/1‑96 (MQ=255)
                                                                      tCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCAtt                       >  2:10320442/1‑96 (MQ=255)
                                                                        aCGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTc                     >  2:2861610/1‑96 (MQ=255)
                                                                         cGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTcc                    >  2:7097810/1‑96 (MQ=255)
                                                                          gggTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTccc                   >  2:13566276/1‑96 (MQ=255)
                                                                           ggTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCg                  <  1:12582640/96‑1 (MQ=255)
                                                                            gTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGa                 <  2:10081480/96‑1 (MQ=255)
                                                                             tCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGAt                <  2:8968822/96‑1 (MQ=255)
                                                                              cAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGAtt               <  2:1342268/96‑1 (MQ=255)
                                                                              cAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGAtt               <  2:5569751/96‑1 (MQ=255)
                                                                               aGTGCGTTGAAGCGCACGGCGTAGCCAGAGACCCGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTc              <  2:6476343/96‑1 (MQ=255)
                                                                                gTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCa             >  1:12287591/1‑96 (MQ=255)
                                                                                 tGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGGTTCAt            >  2:6728334/1‑96 (MQ=255)
                                                                                 tGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCAt            >  1:8253474/1‑96 (MQ=255)
                                                                                  gCGTTGAAGCGCACGGCGTATCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCTGGGTTCTCGATGGCATCCAGCAGCATTTCCCGATTCAtt           >  2:12443253/1‑96 (MQ=255)
                                                                                  gCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCAtt           <  2:2565031/96‑1 (MQ=255)
                                                                                  gCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGGTAGGATATTTTTCCGGGGGCTCGATGGCATCCAGCAGCATTTCCCGATTCAtt           >  1:173469/1‑96 (MQ=37)
                                                                                   cGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTa          >  1:2004314/1‑96 (MQ=255)
                                                                                    gTTGAAGCGCACGGCGTAGCCAGAGACCCGGATTGTCAGGTTGGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTac         <  1:2223027/96‑1 (MQ=25)
                                                                                    gTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCCGCATTTCCCGATTCATTac         <  2:12115372/96‑1 (MQ=255)
                                                                                     ttGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTacg        >  1:4983199/1‑96 (MQ=255)
                                                                                     ttGAAGCGCACGGCGTAGCCAGAGACAAGGATTGGCAGGTTAGGATATTTTTCCGGGTGCTCGATTGCATCCAGCAGCATTTCCCGATTCATTaag        <  1:10264468/96‑3 (MQ=25)
                                                                                      tGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTacgt       >  1:7054147/1‑96 (MQ=255)
                                                                                        aaGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTacgttg     <  2:4192826/96‑1 (MQ=255)
                                                                                        aaGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGGGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTacgttg     >  1:13318386/1‑96 (MQ=255)
                                                                                         aGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTacgttga    >  2:8320642/1‑96 (MQ=255)
                                                                                         aGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTacgttga    >  2:3301389/1‑96 (MQ=255)
                                                                                                |                                                                                          
ATCTTTTTCATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCAC‑GCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACG  >  NC_000913/3257742‑3257927

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 354 total aligned reads displayed.
GATAATCTTTTTCATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑C‑G‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTG  >  NC_000913/3257738‑3257938
                                                                                                       |                                                                                                     
GATAATCTTTTTCATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑N‑N‑N                                                                                                       >  SRR4302518.10350860/1‑101 (MQ=60)
GATAATCTTTTTCATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGGTCACGGGTCAGTGCGTTGAAGCGC‑A‑C‑G                                                                                                       >  SRR4302518.513438/1‑101 (MQ=60)
 ATAATCTTTTTCATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑N‑N‑N                                                                                                     >  SRR4302518.8407178/1‑101 (MQ=60)
 ATAATCTTTTTCATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑C‑G‑G                                                                                                     <  SRR4302518.1152483/101‑1 (MQ=60)
 ATAATCTTTTTCATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑C‑G‑G                                                                                                     <  SRR4302518.7163283/101‑1 (MQ=60)
  TAATCTTTTTCATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑C‑Ggc                                                                                                     >  SRR4302518.6562605/1‑99 (MQ=60)
  NNNTCTTTTTCATACATCCTCCGGGGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑C‑Ggt                                                                                                     <  SRR4302518.9290406/101‑3 (MQ=60)
  NNNTCTTTTTCATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑C‑Ggc                                                                                                     <  SRR4302518.8202920/101‑3 (MQ=60)
  NNNTCTTTTTCATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑C‑Ggc                                                                                                     <  SRR4302518.7875933/101‑3 (MQ=60)
   nnnatttttcatacttcatactgcgtcttcGCGCCTCGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGAGC‑A‑C‑Ggcg                                                                                                    <  SRR4302518.15042385/71‑4 (MQ=60)
    NNNTTTTTCATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGT                                                                                                   <  SRR4302518.8181253/101‑1 (MQ=60)
    ATCTTTTTCATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGCTGAAGCGC‑A‑C‑Ggnnn                                                                                                   >  SRR4302518.10470967/1‑97 (MQ=60)
     TCTTTTTCATACATCCTCCGGCGTAAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTA                                                                                                  <  SRR4302518.2496798/101‑1 (MQ=60)
     NNNTTTTCATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTA                                                                                                  <  SRR4302518.13819245/101‑1 (MQ=60)
         TTTCATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCA                                                                                              <  SRR4302518.2866354/101‑1 (MQ=60)
          TTCATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAG                                                                                             >  SRR4302518.6294789/1‑101 (MQ=60)
          NNNATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAG                                                                                             <  SRR4302518.8028592/101‑1 (MQ=60)
           TCATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGA                                                                                            <  SRR4302518.2091997/101‑1 (MQ=60)
           TCATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGGACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGA                                                                                            >  SRR4302518.5473823/1‑101 (MQ=60)
           NNNTACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGA                                                                                            <  SRR4302518.12128198/101‑1 (MQ=60)
            NNNACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGATCACGGGTCAGTGCGTTGAAACGC‑A‑CGG‑CGTAGCAAGAG                                                                                           <  SRR4302518.13221413/101‑1 (MQ=60)
            CGTACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGATGGGC‑A‑CGG‑CGTAGCCANNN                                                                                           >  SRR4302518.12642286/1‑101 (MQ=60)
            CATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAG                                                                                           >  SRR4302518.2143830/1‑101 (MQ=60)
             ATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGNNN                                                                                          >  SRR4302518.13749040/1‑101 (MQ=60)
             ATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGA                                                                                          <  SRR4302518.4866290/101‑1 (MQ=60)
              TACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGAC                                                                                         <  SRR4302518.1874788/101‑1 (MQ=60)
               NNNTCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAGCATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGAAA                                                                                        <  SRR4302518.12415870/101‑1 (MQ=60)
               NNNTCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑AGCAGCCAGAGACA                                                                                        <  SRR4302518.14042381/101‑1 (MQ=60)
               ACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACA                                                                                        >  SRR4302518.1112772/1‑101 (MQ=60)
               ACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACA                                                                                        <  SRR4302518.1672032/101‑1 (MQ=60)
               ACATCCGCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTACTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACA                                                                                        <  SRR4302518.2636398/101‑1 (MQ=60)
                NNNCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACGC                                                                                       <  SRR4302518.14971421/101‑1 (MQ=60)
                NNNCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACAC                                                                                       <  SRR4302518.13436474/101‑1 (MQ=60)
                CATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACAC                                                                                       >  SRR4302518.5688376/1‑101 (MQ=60)
                CATCCTCCGGCGTCAGAGCGACTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACAC                                                                                       <  SRR4302518.5776867/101‑1 (MQ=60)
                 NNNCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACG                                                                                      <  SRR4302518.10978590/101‑1 (MQ=60)
                  TCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGG                                                                                     <  SRR4302518.3274946/101‑1 (MQ=60)
                   CCTCCGGCGTCAGAGCGCCTGGGTAAAGGAACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGGAGCCAGAGACACGGC                                                                                    >  SRR4302518.1861192/1‑101 (MQ=60)
                     TCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGNNN                                                                                  >  SRR4302518.12729112/1‑101 (MQ=60)
                     TCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATT                                                                                  <  SRR4302518.5464705/101‑1 (MQ=60)
                     TCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCGGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATT                                                                                  <  SRR4302518.6743715/101‑1 (MQ=60)
                     TCCGGCGTCAGAGCGCCTGGATAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTGGCCAGAGACACGGATT                                                                                  >  SRR4302518.3829495/1‑101 (MQ=60)
                     NNNGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATC                                                                                  <  SRR4302518.9299112/101‑1 (MQ=60)
                       CGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑AGTAGCCAGAGACACGGATTGT                                                                                <  SRR4302518.3869117/101‑1 (MQ=60)
                        NNNGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGct                                                                               <  SRR4302518.11846726/101‑3 (MQ=60)
                        GGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTNNN                                                                               >  SRR4302518.8247107/1‑101 (MQ=60)
                         NNNTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCA                                                                              <  SRR4302518.8383683/101‑1 (MQ=60)
                         GCGTCAGAGCCCCTCGGTCAAGGTACGTCAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGCTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCA                                                                              <  SRR4302518.7134828/101‑1 (MQ=60)
                          NNNCAGAGAGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAG                                                                             <  SRR4302518.14586205/101‑1 (MQ=60)
                          CGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAG                                                                             >  SRR4302518.2033592/1‑101 (MQ=60)
                          CGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGA‑T‑CGG‑CGTAGCCAGAGACACGGATTGTNNN                                                                             >  SRR4302518.10537739/1‑101 (MQ=60)
                           ctcacagacactcggttacggtaaGTGAAATAACCTCCTGTTGATGTTCACGGGTCAGTGCGTTAAAGCGC‑A‑CGG‑CGTAGCCCGAAACACGGATTGTCAGG                                                                            <  SRR4302518.3356485/77‑1 (MQ=50)
                           GTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGG                                                                            >  SRR4302518.458973/1‑101 (MQ=60)
                             TAGAGCGCCTGGGTAAAGGTCCGTGAAATAACATTCTTTTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑C‑CGG‑CGTAGCCAGAGGCACGGATTGTCAGGTT                                                                          <  SRR4302518.128429/101‑1 (MQ=60)
                             CAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTT                                                                          <  SRR4302518.6091533/101‑1 (MQ=60)
                             CACAGCGCCTGGGTAAAGGTACGTGAAATAACCTCCTGTTGCCATTCACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACCCGGATTGTCAGGTT                                                                          <  SRR4302518.2378026/101‑1 (MQ=60)
                              NNNGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTA                                                                         <  SRR4302518.14140977/101‑1 (MQ=60)
                              AGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGATA                                                                         >  SRR4302518.5801169/1‑101 (MQ=60)
                               NNNCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAG                                                                        <  SRR4302518.8780247/101‑1 (MQ=60)
                                GGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTATCCAGAGACACGGATTGTCAGGGTAGG                                                                       >  SRR4302518.4896290/1‑101 (MQ=60)
                                AGCGCCTGGGTAAAGGTACCTGAAATAACATCCTGTTGCTGTTCAGGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑GGTAGCCAGAGACACGGATTGTCggggtggg                                                                       >  SRR4302518.3298211/1‑93 (MQ=60)
                                  NNNCTGGGTAAAGGTACGTTAAATAACATCCTGTTTCTGTTCACGCGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGCATTGTCAGGTTAGGAT                                                                     <  SRR4302518.13367769/101‑1 (MQ=60)
                                  CGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGAT                                                                     >  SRR4302518.5587712/1‑101 (MQ=60)
                                   GCCTGGGTACACGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATA                                                                    <  SRR4302518.728177/101‑1 (MQ=60)
                                   GCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGGCAGGTTAGGAGA                                                                    >  SRR4302518.411023/1‑101 (MQ=60)
                                    CGTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACTCGGATTGTCAGGTTAGGANNN                                                                   >  SRR4302518.13409563/1‑101 (MQ=60)
                                     NNNGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATACT                                                                  <  SRR4302518.12306715/101‑1 (MQ=60)
                                     CTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATT                                                                  <  SRR4302518.5435422/101‑1 (MQ=60)
                                     CTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGGGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATT                                                                  >  SRR4302518.6011251/1‑101 (MQ=60)
                                      TGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTT                                                                 >  SRR4302518.3629121/1‑101 (MQ=60)
                                       GGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTT                                                                <  SRR4302518.4385156/101‑1 (MQ=60)
                                       GGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTT                                                                <  SRR4302518.5373807/101‑1 (MQ=60)
                                       GGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGGTAGGATATTTT                                                                >  SRR4302518.7157829/1‑101 (MQ=60)
                                        GGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTT                                                               <  SRR4302518.3671566/101‑1 (MQ=60)
                                         GTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGGCGGGTTAGGATATTTTTC                                                              <  SRR4302518.5363930/101‑1 (MQ=60)
                                          TAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCC                                                             >  SRR4302518.3327116/1‑101 (MQ=60)
                                          TAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCC                                                             >  SRR4302518.1857032/1‑101 (MQ=60)
                                          TAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTNNN                                                             >  SRR4302518.7701503/1‑101 (MQ=60)
                                          TAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAAGATATTTTNNN                                                             >  SRR4302518.11262143/1‑101 (MQ=60)
                                           CAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTNNN                                                            >  SRR4302518.14422742/1‑101 (MQ=60)
                                            NNNGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGG                                                           <  SRR4302518.10221928/101‑1 (MQ=60)
                                            NNNGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGG                                                           <  SRR4302518.13739279/101‑1 (MQ=60)
                                            AAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGG                                                           >  SRR4302518.65802/1‑101 (MQ=60)
                                              NNNACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGT                                                         <  SRR4302518.8316152/101‑1 (MQ=60)
                                              GGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGT                                                         <  SRR4302518.3994184/101‑1 (MQ=60)
                                              GGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGT                                                         >  SRR4302518.5899662/1‑101 (MQ=60)
                                               NNNCGTGAACTAACAGCCGGTTGCTGTTCACGGGTTAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAAACACGTATTGTCAGGTTAGGATATTTTTCCGGGTG                                                        <  SRR4302518.12171563/101‑1 (MQ=60)
                                               GTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTACGGNNN                                                        >  SRR4302518.14344050/1‑101 (MQ=60)
                                                 ACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCT                                                      >  SRR4302518.2786002/1‑101 (MQ=60)
                                                  NNNGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTATCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTC                                                     <  SRR4302518.13623250/101‑1 (MQ=60)
                                                  NNNGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTC                                                     <  SRR4302518.9542702/101‑1 (MQ=60)
                                                  CGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTC                                                     <  SRR4302518.2831899/101‑1 (MQ=60)
                                                  CGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTC                                                     >  SRR4302518.5611385/1‑101 (MQ=60)
                                                   NNNAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCG                                                    <  SRR4302518.14624719/101‑1 (MQ=60)
                                                     nnnctcttccgatcTTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGAT                                                  <  SRR4302518.11716208/87‑1 (MQ=60)
                                                     GGAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCNNN                                                  >  SRR4302518.11148266/1‑101 (MQ=60)
                                                     GAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGAT                                                  >  SRR4302518.173556/1‑101 (MQ=60)
                                                     GAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGAT                                                  >  SRR4302518.2147012/1‑101 (MQ=60)
                                                     GAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGAT                                                  >  SRR4302518.3481538/1‑101 (MQ=60)
                                                     GAAATAACATCCTGTTGCTGTTCACGGGTAAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCNNN                                                  >  SRR4302518.11452911/1‑101 (MQ=60)
                                                      NNNTAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATG                                                 <  SRR4302518.8128887/101‑1 (MQ=60)
                                                        NNNACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGC                                               <  SRR4302518.7405228/101‑1 (MQ=60)
                                                         TAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGNNN                                              >  SRR4302518.9042390/1‑101 (MQ=60)
                                                         TAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGNNN                                              >  SRR4302518.13979948/1‑101 (MQ=60)
                                                         NNNCATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGAA                                              <  SRR4302518.7761028/101‑1 (MQ=60)
                                                          NNNATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCGAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCAT                                             <  SRR4302518.12972896/101‑1 (MQ=60)
                                                          NNNATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCAT                                             <  SRR4302518.7689143/101‑1 (MQ=60)
                                                          AACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCAT                                             <  SRR4302518.3780990/101‑1 (MQ=60)
                                                          AACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCAT                                             <  SRR4302518.3800901/101‑1 (MQ=60)
                                                           nnngCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATC                                            <  SRR4302518.7651282/97‑1 (MQ=60)
                                                           ACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCNNN                                            >  SRR4302518.9203748/1‑101 (MQ=60)
                                                            CTTCCTGTTGCTGTTCACGGGT‑AGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATNNN                                          >  SRR4302518.12404581/1‑101 (MQ=60)
                                                            CATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCC                                           >  SRR4302518.4808267/1‑101 (MQ=60)
                                                            CATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGACAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCC                                           <  SRR4302518.3636780/101‑1 (MQ=60)
                                                             NNNCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCA                                          <  SRR4302518.9460908/101‑1 (MQ=60)
                                                             ATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGGTAGGATATTTTTCCGGGTGCTCGATGGCATNNN                                          >  SRR4302518.7428992/1‑101 (MQ=60)
                                                             AACCTGTTTCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATNNN                                          >  SRR4302518.11010358/1‑101 (MQ=60)
                                                              TTCTGTTGCTGTTCCCGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAG                                         <  SRR4302518.4682970/101‑1 (MQ=60)
                                                              TCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAG                                         >  SRR4302518.3713928/1‑101 (MQ=60)
                                                              NNNTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAG                                         <  SRR4302518.9592558/101‑1 (MQ=60)
                                                               CCTGTTGCTGTTCACGGGTCAGTGCGTTGATGCGC‑A‑C‑GACGTAGCTAGAGACACGGAGTGTCAGGTTAGGATATTGTTCCGGGggttcgatggttttcagc                                        >  SRR4302518.5626983/1‑83 (MQ=60)
                                                               CCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCNNN                                        >  SRR4302518.10103625/1‑101 (MQ=60)
                                                               CCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCNNN                                        >  SRR4302518.9733435/1‑101 (MQ=60)
                                                                CTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCATGGTAGGATATTTGTACGGGTGCTCGATGGCCTCCAGCA                                       >  SRR4302518.1440444/1‑101 (MQ=60)
                                                                CTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCANNN                                       >  SRR4302518.14197598/1‑101 (MQ=60)
                                                                 TGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGNNN                                      >  SRR4302518.14132484/1‑101 (MQ=60)
                                                                 TGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGCGCTCGATGGCATCCAGCAG                                      >  SRR4302518.5858946/1‑101 (MQ=60)
                                                                  cagggatggaatctaaCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCNNN                                     >  SRR4302518.11470006/17‑101 (MQ=60)
                                                                  NNNGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGC                                     <  SRR4302518.9383079/101‑1 (MQ=60)
                                                                  GTTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCTGGTGCTCGATGGCATCAAGCAGC                                     >  SRR4302518.7077039/1‑101 (MQ=60)
                                                                   TTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCA                                    <  SRR4302518.6248986/101‑1 (MQ=60)
                                                                    TGCTGTTCACGAGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCAT                                   <  SRR4302518.1266897/101‑1 (MQ=60)
                                                                    NNNTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCAT                                   <  SRR4302518.7680046/101‑1 (MQ=60)
                                                                     GCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATT                                  >  SRR4302518.6637/1‑101 (MQ=60)
                                                                      CTGTGCGCGGGTCAGTGCGTTGAAGCGCTA‑G‑G‑CGTAGCCAGAGACCCGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTT                                 <  SRR4302518.2260929/101‑1 (MQ=60)
                                                                       cgtcccCGGGTCAGTGCGTGGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTC                                <  SRR4302518.4572212/95‑1 (MQ=60)
                                                                       TGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGTGAGAGACACGGATTGTCAGGGTAGGATATTTTTCagggatctcggagggatccagcagtatnnn                                >  SRR4302518.8689071/1‑71 (MQ=60)
                                                                       TGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTC                                <  SRR4302518.2789447/101‑1 (MQ=60)
                                                                         TTCACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTNNN                              >  SRR4302518.10447418/1‑101 (MQ=60)
                                                                         NNNACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCC                              <  SRR4302518.13920602/101‑1 (MQ=60)
                                                                          NNNCGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCG                             <  SRR4302518.13172491/101‑1 (MQ=60)
                                                                          NNNCGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCG                             <  SRR4302518.12590539/101‑1 (MQ=60)
                                                                           CACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCNNN                            >  SRR4302518.8901679/1‑101 (MQ=60)
                                                                           CACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGN                            >  SRR4302518.4861260/1‑101 (MQ=60)
                                                                            ACGGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGAT                           <  SRR4302518.4035382/101‑1 (MQ=60)
                                                                              GGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTC                         >  SRR4302518.1048445/1‑101 (MQ=60)
                                                                              GGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGANNN                         >  SRR4302518.10317862/1‑101 (MQ=60)
                                                                              GGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATCTCCCGANNN                         >  SRR4302518.12711864/1‑101 (MQ=60)
                                                                              GGGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGAATATTTTTCCTGGGGCTCGATGGCATCCATCAGCGTTTCGCGANNN                         >  SRR4302518.10912693/1‑101 (MQ=60)
                                                                               NNNCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCA                        <  SRR4302518.8366588/101‑1 (MQ=60)
                                                                               GGTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCA                        <  SRR4302518.709684/101‑1 (MQ=60)
                                                                                NNNAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCAT                       <  SRR4302518.8442261/101‑1 (MQ=60)
                                                                                GTCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCAT                       >  SRR4302518.3032189/1‑101 (MQ=60)
                                                                                 GCAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATT                      <  SRR4302518.3578917/101‑1 (MQ=60)
                                                                                  TAGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTA                     <  SRR4302518.6445974/101‑1 (MQ=60)
                                                                                  NNNTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTA                     <  SRR4302518.12292965/101‑1 (MQ=60)
                                                                                   AGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACCCGGATTGTCAGGTTGGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTAC                    <  SRR4302518.2330202/101‑1 (MQ=60)
                                                                                   AGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTAC                    >  SRR4302518.6780313/1‑101 (MQ=60)
                                                                                   AGTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTAC                    <  SRR4302518.4027271/101‑1 (MQ=60)
                                                                                    nnngcTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACAAGGATTGGCAGGTTAGGATATTTTTCCGGGTGCTCGATTGCATCCAGCAGCATTTCCCGATTCATTAAG                   <  SRR4302518.10748856/96‑1 (MQ=60)
                                                                                    NNNCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACG                   <  SRR4302518.9016732/101‑1 (MQ=60)
                                                                                    GTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTNNN                   >  SRR4302518.9433053/1‑101 (MQ=60)
                                                                                    GTGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTNNN                   >  SRR4302518.12080403/1‑101 (MQ=60)
                                                                                     TGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTANNN                  >  SRR4302518.8660616/1‑101 (MQ=60)
                                                                                     TGCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTANNN                  >  SRR4302518.9087586/1‑101 (MQ=60)
                                                                                      NNNTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTT                 <  SRR4302518.11839416/101‑1 (MQ=60)
                                                                                      GCGTTGAAGCGC‑ATG‑G‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTccnnn                 >  SRR4302518.11490394/1‑96 (MQ=60)
                                                                                      GCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACNNN                 >  SRR4302518.8859905/1‑101 (MQ=60)
                                                                                      GCGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGGTAGGATATTTTTCCGGGGGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTT                 >  SRR4302518.180514/1‑101 (MQ=60)
                                                                                       CGTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTG                >  SRR4302518.5288534/1‑101 (MQ=60)
                                                                                       CGTTAACGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTG                <  SRR4302518.2974517/101‑1 (MQ=60)
                                                                                        GTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGA               <  SRR4302518.1290145/101‑1 (MQ=60)
                                                                                        GTTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTNNN               >  SRR4302518.13073630/1‑101 (MQ=60)
                                                                                         TTGAAGGGC‑A‑CGG‑CGTAGCCCGAGACCCGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGAC              <  SRR4302518.1954024/101‑1 (MQ=60)
                                                                                         TTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGAC              >  SRR4302518.2200529/1‑101 (MQ=60)
                                                                                         TTGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGAC              >  SRR4302518.5242112/1‑101 (MQ=60)
                                                                                         NNNAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGAC              <  SRR4302518.11293142/101‑1 (MQ=60)
                                                                                          TGAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGNNN             >  SRR4302518.7427315/1‑101 (MQ=60)
                                                                                           GAAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGTAGCATTTCCCGATTCATTACGTTGANNN            >  SRR4302518.10558064/1‑101 (MQ=60)
                                                                                           GAAGCGC‑A‑CGG‑CGTAGCCAGAAACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGCTGANNN            >  SRR4302518.10897843/1‑101 (MQ=60)
                                                                                            AAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATCTTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTT           <  SRR4302518.6817507/101‑1 (MQ=60)
                                                                                            AAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTT           >  SRR4302518.1974584/1‑101 (MQ=60)
                                                                                            AAGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGGGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACNNN           >  SRR4302518.13931542/1‑101 (MQ=60)
                                                                                             AGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGNNN          >  SRR4302518.10919732/1‑101 (MQ=60)
                                                                                             AGCGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGNNN          >  SRR4302518.8024462/1‑101 (MQ=60)
                                                                                               nnngcactG‑CGTAGCAAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTG          <  SRR4302518.8440977/93‑1 (MQ=60)
                                                                                               CGC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAG        <  SRR4302518.863318/101‑1 (MQ=60)
                                                                                                GC‑A‑CGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGGGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGNNN       >  SRR4302518.13893289/1‑101 (MQ=60)
                                                                                                  nnncgG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGAGTGCTCGATGGCATCCCGCAGCATTTCCCGATTCATTACGTTGACGTTGAGG       <  SRR4302518.8551051/96‑1 (MQ=60)
                                                                                                   nnngG‑CGTAGCCAGAGACTCGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGAT      <  SRR4302518.12011214/97‑1 (MQ=60)
                                                                                                   nnngG‑CGTAGCCAGAGACACGTATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGT      <  SRR4302518.10810175/97‑1 (MQ=60)
                                                                                                   N‑N‑N‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGTCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGT    <  SRR4302518.8577786/101‑1 (MQ=60)
                                                                                                   N‑N‑N‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGT    <  SRR4302518.14852510/101‑1 (MQ=60)
                                                                                                    acgG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTG     <  SRR4302518.3196993/98‑1 (MQ=60)
                                                                                                     cgG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGT    >  SRR4302518.2300118/3‑101 (MQ=60)
                                                                                                     cgG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTACCGATTCATTACGTTGACGTTGAGGNNN    >  SRR4302518.10591612/3‑101 (MQ=60)
                                                                                                     cgG‑CGTAGCCAGAGACACGGATTGTCAGGGTAGGATATTTTTCCGGGGGATCGTTGGCATCCAGCAGTATTTCCCGATTGATTACGTAGACGCTGAGGTGT    >  SRR4302518.1572625/3‑101 (MQ=60)
                                                                                                     G‑G‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGggtg   >  SRR4302518.6848918/1‑97 (MQ=60)
                                                                                                     G‑G‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTAATTACGTTGACGTTGAGGTGTT   >  SRR4302518.5196387/1‑101 (MQ=60)
                                                                                                       N‑NNTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTG  <  SRR4302518.12980102/101‑1 (MQ=60)
                                                                                                       |                                                                                                     
GATAATCTTTTTCATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGC‑A‑C‑G‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTG  >  NC_000913/3257738‑3257938

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑