Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 884 257.2 12958910 96.9% 12557183 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,257,838 +G coding (2222/2241 nt) tdcE ← putative formate acetyltransferase 3

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,257,8371.G100.0% 820.2 / NA 234A741A (GCG→GCC) tdcEputative formate acetyltransferase 3
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (121/113);  total (121/113)

Only 100 of 247 total aligned reads displayed.

TTTTTCATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCAC‑GCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTT  >  NC_000913/3257745‑3257923
                                                                                             |                                                                                      
tttttCATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCAc                                                                                         <  2:372957/93‑1 (MQ=255)
  tttCATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCAc‑gg                                                                                      <  1:3199065/93‑2 (MQ=255)
  tttCATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCAc‑gg                                                                                      >  1:310203/1‑92 (MQ=255)
     cATACACCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCAc‑ggcgt                                                                                   <  1:2315830/93‑5 (MQ=255)
        aCATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGc                                                                                 >  2:6409377/1‑93 (MQ=255)
        aCATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGc                                                                                 <  1:4654377/93‑1 (MQ=255)
         caccctccGGCGTCAGAGCGCATGGGTAAAGGTACGTGAAATACCATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCCCGGCGTAGcc                                                                                <  1:3117086/90‑1 (MQ=255)
         cATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGcc                                                                                <  1:5724834/93‑1 (MQ=255)
         cATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGcc                                                                                <  1:2201531/93‑1 (MQ=255)
         cATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGcc                                                                                >  1:1352660/1‑93 (MQ=255)
          aTCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCa                                                                               >  2:6030191/1‑93 (MQ=255)
          aTCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCa                                                                               >  2:4381656/1‑93 (MQ=255)
          aTCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCa                                                                               >  2:5656182/1‑93 (MQ=255)
           tcctccGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCag                                                                              >  1:4219754/1‑93 (MQ=255)
           tcctccGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCag                                                                              >  1:5208319/1‑93 (MQ=255)
            cctccGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCaga                                                                             <  2:1959391/93‑1 (MQ=255)
              tccGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAgaga                                                                           <  1:5112816/93‑1 (MQ=255)
                cGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGaca                                                                         >  1:4381376/1‑93 (MQ=255)
                 ggCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGacac                                                                        <  1:6466485/93‑1 (MQ=255)
                 ggCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGacac                                                                        <  2:1585230/93‑1 (MQ=255)
                  gCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGATACACg                                                                       >  2:3231910/1‑93 (MQ=255)
                  gCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACg                                                                       >  2:5521235/1‑93 (MQ=255)
                  gCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACg                                                                       >  2:5717669/1‑93 (MQ=255)
                  gCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACg                                                                       >  1:548737/1‑93 (MQ=255)
                   cGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACgg                                                                      <  1:5338495/93‑1 (MQ=255)
                    gTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGa                                                                     >  1:5511778/1‑93 (MQ=255)
                    gTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGa                                                                     <  2:3985775/93‑1 (MQ=255)
                    gTCAGAGCGCCTGGGTAAAGGCACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGACGCGCACGGCGTAGCCAGCGACACGGa                                                                     >  1:4982530/1‑93 (MQ=255)
                     tCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGAt                                                                    >  2:4979307/1‑93 (MQ=255)
                     tCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGAt                                                                    >  2:1998489/1‑93 (MQ=255)
                       agagCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTg                                                                  <  2:3324723/93‑1 (MQ=255)
                       agagCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTg                                                                  <  2:891072/93‑1 (MQ=255)
                          gcgcCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCa                                                               <  1:4032910/93‑1 (MQ=255)
                          gcgcCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCa                                                               <  2:5842383/93‑1 (MQ=255)
                              cTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGtt                                                           >  2:1805169/1‑93 (MQ=255)
                              cTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGtt                                                           >  1:5131760/1‑93 (MQ=255)
                              cTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGtt                                                           <  1:289831/93‑1 (MQ=255)
                              cTGGGTAAAGGTACGTGAAATAACATCCTGTTACTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGtt                                                           >  2:3978647/1‑93 (MQ=255)
                               tGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTa                                                          >  1:6415589/1‑93 (MQ=255)
                                gggTAAAGGTACGTGAAATAACATCCTCTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGGTAg                                                         >  1:101069/1‑93 (MQ=255)
                                  gTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGa                                                       <  2:1323607/93‑1 (MQ=255)
                                    aaaGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGata                                                     <  2:3196981/93‑1 (MQ=255)
                                    aaaGGTAAGTGAAATAACATCCTGTTGCGGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGata                                                     <  2:4680052/93‑1 (MQ=255)
                                     aaGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGatat                                                    >  1:4999762/1‑93 (MQ=255)
                                     aaGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGatat                                                    >  2:881157/1‑93 (MQ=255)
                                      aGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATAtt                                                   <  1:6035896/93‑1 (MQ=255)
                                       ggTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATAttt                                                  >  2:1153652/1‑93 (MQ=255)
                                       ggTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATAttt                                                  >  2:5805844/1‑93 (MQ=255)
                                       ggTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATAttt                                                  >  1:2442464/1‑93 (MQ=255)
                                        gTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATAtttt                                                 >  2:5232300/1‑93 (MQ=255)
                                        gTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATAtttt                                                 >  2:3018260/1‑93 (MQ=255)
                                         tACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATAttttt                                                >  1:1211530/1‑93 (MQ=255)
                                          aCGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTc                                               <  1:4583788/93‑1 (MQ=255)
                                          aCGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTc                                               >  2:3667013/1‑93 (MQ=255)
                                             tGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGGCAGGTTAGGATATTTTTCCgg                                            >  2:4138669/1‑93 (MQ=255)
                                              gAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCggg                                           <  1:4904801/93‑1 (MQ=255)
                                               aaaTAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGt                                          >  2:171535/1‑93 (MQ=255)
                                                aaTAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTg                                         <  1:947292/93‑1 (MQ=255)
                                                  tAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCt                                       <  2:427455/93‑1 (MQ=255)
                                                  tAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCt                                       >  1:1130696/1‑93 (MQ=255)
                                                   aaCATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTc                                      <  2:608993/93‑1 (MQ=255)
                                                   aaCATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTc                                      >  2:5404993/1‑93 (MQ=255)
                                                   aaCATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTc                                      >  1:5224735/1‑93 (MQ=255)
                                                         cTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGc                                >  2:1207944/1‑93 (MQ=255)
                                                          tGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCa                               <  2:339228/93‑1 (MQ=255)
                                                            ttGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATc                             <  1:5941155/93‑1 (MQ=255)
                                                            ttGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATc                             >  2:2878292/1‑93 (MQ=255)
                                                             tGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATcc                            <  1:5660391/93‑1 (MQ=255)
                                                             tGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATcc                            <  1:601208/93‑1 (MQ=255)
                                                             tGCTGTGCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGAATATTTTTCCGGGTGCTCGATGGCATcc                            <  2:1296182/93‑1 (MQ=255)
                                                              gCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCca                           >  2:5082587/1‑93 (MQ=255)
                                                              gCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCca                           >  2:5287172/1‑93 (MQ=255)
                                                               cTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCcag                          >  2:5201111/1‑93 (MQ=255)
                                                               cTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCcag                          >  1:3458602/1‑93 (MQ=255)
                                                                tGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCcagc                         <  1:1561728/93‑1 (MQ=255)
                                                                tGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCcagc                         >  2:1549106/1‑93 (MQ=255)
                                                                tGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCcagc                         <  2:2072252/93‑1 (MQ=255)
                                                                 gtgcACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCcagca                        <  2:1116376/90‑1 (MQ=255)
                                                                 gTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCcagca                        <  2:677971/93‑1 (MQ=255)
                                                                 gTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCcagca                        <  2:2461331/93‑1 (MQ=255)
                                                                  ttCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCcagcag                       <  2:3922515/93‑1 (MQ=255)
                                                                    cACGTGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAgcagca                     <  1:882246/93‑1 (MQ=255)
                                                                      cGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCAtt                   <  1:3954654/93‑1 (MQ=255)
                                                                      cGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCAtt                   <  1:1754988/93‑1 (MQ=255)
                                                                       gggTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTGGATGGCATCCAGCAGCAttt                  <  1:2786828/93‑1 (MQ=255)
                                                                       gggTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCCttt                  >  1:1867757/1‑93 (MQ=255)
                                                                       gcgTCAGTGCGGTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCAttt                  <  1:1378069/91‑1 (MQ=37)
                                                                        ggTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTc                 >  2:4232746/1‑93 (MQ=255)
                                                                         gTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTcc                <  2:3065357/93‑1 (MQ=255)
                                                                         gTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTcc                >  2:1966754/1‑93 (MQ=255)
                                                                          tCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTccc               <  1:2605043/93‑1 (MQ=255)
                                                                          tCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTccc               <  1:1949842/93‑1 (MQ=255)
                                                                            aGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGa             >  1:4475135/1‑93 (MQ=255)
                                                                             gTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGAt            >  1:727034/1‑93 (MQ=255)
                                                                              tGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGAtt           >  2:403949/1‑93 (MQ=255)
                                                                               gCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTc          >  2:2509257/1‑93 (MQ=255)
                                                                                  ttGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCAtt       <  1:5785105/93‑1 (MQ=255)
                                                                                   tGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTa      <  2:3561945/93‑1 (MQ=255)
                                                                                    gAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTac     <  1:3898292/93‑1 (MQ=255)
                                                                                      aGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTacgt   <  1:5453723/93‑1 (MQ=255)
                                                                                             |                                                                                      
TTTTTCATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCAC‑GCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTT  >  NC_000913/3257745‑3257923

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTTTTCATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCAC‑G‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGA  >  NC_000913/3257745‑3257931
                                                                                              |                                                                                              
TTTTTCATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCAC‑G                                                                                                <  SRR4302534.2319214/94‑1 (MQ=60)
 TTTTCATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCAC‑G‑G                                                                                              >  SRR4302534.318211/1‑94 (MQ=60)
  TTTCATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCAC‑Ggc                                                                                              <  SRR4302534.3283879/94‑3 (MQ=60)
     CATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTA                                                                                           >  SRR4302534.786720/1‑94 (MQ=60)
     CATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTA                                                                                           >  SRR4302534.4692078/1‑94 (MQ=60)
     CATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTA                                                                                           <  SRR4302534.5827416/94‑1 (MQ=60)
     CATACACCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTA                                                                                           <  SRR4302534.2377286/94‑1 (MQ=60)
      ATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAG                                                                                          <  SRR4302534.6141650/94‑1 (MQ=60)
      ATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAG                                                                                          <  SRR4302534.5282568/94‑1 (MQ=60)
      ATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAG                                                                                          <  SRR4302534.1995561/94‑1 (MQ=60)
      ATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAAAATCCTGTTGCTGTTCACGGGTGAGTGCGTTGGAGCGCAC‑Ggcggag                                                                                          >  SRR4302534.367753/1‑88 (MQ=60)
        ACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCC                                                                                        <  SRR4302534.4780731/94‑1 (MQ=60)
        ACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCC                                                                                        >  SRR4302534.1388315/1‑94 (MQ=60)
         CATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCA                                                                                       <  SRR4302534.2259785/94‑1 (MQ=60)
         CATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCA                                                                                       <  SRR4302534.5879928/94‑1 (MQ=60)
         CACCCTCCGGCGTCAGAGCGCATGGGTAAAGGTACGTGAAATACCATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCCCGG‑CGTAGCCA                                                                                       <  SRR4302534.3199045/94‑1 (MQ=60)
          ATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAG                                                                                      >  SRR4302534.5688194/1‑94 (MQ=60)
          ATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAG                                                                                      >  SRR4302534.425655/1‑94 (MQ=60)
          ATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAG                                                                                      >  SRR4302534.4333514/1‑94 (MQ=60)
          ATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAG                                                                                      >  SRR4302534.5348870/1‑94 (MQ=60)
          ATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAG                                                                                      >  SRR4302534.1457608/1‑94 (MQ=60)
            CCTTCGGCGTCAGAGGGCCTGGGTAAATGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAG                                                                                    <  SRR4302534.4396979/94‑1 (MQ=60)
            CCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAG                                                                                    >  SRR4302534.4108678/1‑94 (MQ=60)
              TCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGAC                                                                                  <  SRR4302534.5250797/94‑1 (MQ=60)
              TCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGAC                                                                                  >  SRR4302534.5261031/1‑94 (MQ=60)
               CCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACA                                                                                 <  SRR4302534.4756646/94‑1 (MQ=60)
               CCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACA                                                                                 >  SRR4302534.4499482/1‑94 (MQ=60)
                CGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACAC                                                                                <  SRR4302534.5908291/94‑1 (MQ=60)
                 GGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACG                                                                               >  SRR4302534.6084487/1‑94 (MQ=60)
                 GGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACG                                                                               <  SRR4302534.6640656/94‑1 (MQ=60)
                 GGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACG                                                                               >  SRR4302534.562274/1‑94 (MQ=60)
                 GGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACG                                                                               >  SRR4302534.3058999/1‑94 (MQ=60)
                  GCGTCAGAGCGGCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGG                                                                              <  SRR4302534.1337408/94‑1 (MQ=60)
                   CGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGA                                                                             <  SRR4302534.5482420/94‑1 (MQ=60)
                   CGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGA                                                                             >  SRR4302534.5660467/1‑94 (MQ=60)
                   CGTCAGAGCGCCTGGGTAAAGGCACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGACGCGCACGG‑CGTAGCCAGCGACACGGA                                                                             >  SRR4302534.5117369/1‑94 (MQ=60)
                     TCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATT                                                                           <  SRR4302534.2535261/94‑1 (MQ=60)
                     TCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATT                                                                           <  SRR4302534.4856889/94‑1 (MQ=60)
                      CAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCGAGAGACACGGATTG                                                                          <  SRR4302534.1786548/94‑1 (MQ=60)
                      CAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTG                                                                          <  SRR4302534.2776811/94‑1 (MQ=60)
                         AGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCA                                                                       <  SRR4302534.4754984/94‑1 (MQ=60)
                          GCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAG                                                                      <  SRR4302534.4141018/94‑1 (MQ=60)
                          GCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAG                                                                      <  SRR4302534.4588920/94‑1 (MQ=60)
                           CGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGG                                                                     >  SRR4302534.5583178/1‑94 (MQ=60)
                           CGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGG                                                                     >  SRR4302534.5355611/1‑94 (MQ=60)
                           CGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTGCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGG                                                                     <  SRR4302534.5620383/94‑1 (MQ=60)
                             CCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTT                                                                   >  SRR4302534.877009/1‑94 (MQ=60)
                             CCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTT                                                                   >  SRR4302534.5270298/1‑94 (MQ=60)
                             CCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTT                                                                   >  SRR4302534.5123148/1‑94 (MQ=60)
                              CTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTA                                                                  >  SRR4302534.6588684/1‑94 (MQ=60)
                              CTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTA                                                                  <  SRR4302534.297328/94‑1 (MQ=60)
                              CTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTA                                                                  <  SRR4302534.6176103/94‑1 (MQ=60)
                              CTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTA                                                                  >  SRR4302534.1274003/1‑94 (MQ=60)
                               TGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAG                                                                 >  SRR4302534.2038126/1‑94 (MQ=60)
                               TGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAG                                                                 <  SRR4302534.3440154/94‑1 (MQ=60)
                               TGGGTAAAGGTACGTGAAATAACATCCTCTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGGTAG                                                                 >  SRR4302534.103766/1‑94 (MQ=60)
                                 GGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGA                                                               >  SRR4302534.2808881/1‑94 (MQ=60)
                                   TAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATA                                                             <  SRR4302534.504414/94‑1 (MQ=60)
                                    AAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATAT                                                            >  SRR4302534.564152/1‑94 (MQ=60)
                                    AAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATAT                                                            >  SRR4302534.5134938/1‑94 (MQ=60)
                                    AAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGGTAGGATAT                                                            >  SRR4302534.1965452/1‑94 (MQ=60)
                                     AAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATT                                                           >  SRR4302534.4605956/1‑94 (MQ=60)
                                      AGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTT                                                          <  SRR4302534.4524198/94‑1 (MQ=60)
                                      AGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTT                                                          >  SRR4302534.2507379/1‑94 (MQ=60)
                                      AGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTT                                                          <  SRR4302534.6199937/94‑1 (MQ=60)
                                      AGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTT                                                          <  SRR4302534.1903852/94‑1 (MQ=60)
                                       GGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTT                                                         <  SRR4302534.6194582/94‑1 (MQ=60)
                                        GTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTT                                                        >  SRR4302534.673337/1‑94 (MQ=60)
                                        GTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTT                                                        >  SRR4302534.1243413/1‑94 (MQ=60)
                                        GTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTT                                                        >  SRR4302534.924368/1‑94 (MQ=60)
                                         TACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTC                                                       <  SRR4302534.2486208/94‑1 (MQ=60)
                                          ACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCC                                                      <  SRR4302534.4708160/94‑1 (MQ=60)
                                           CGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCG                                                     <  SRR4302534.2444844/94‑1 (MQ=60)
                                           CGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCG                                                     <  SRR4302534.3403491/94‑1 (MQ=60)
                                             TGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGG                                                   >  SRR4302534.3273385/1‑94 (MQ=60)
                                             TGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGG                                                   >  SRR4302534.1233437/1‑94 (MQ=60)
                                             TGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGGTAGGATATTTTTtaggg                                                   >  SRR4302534.140255/1‑89 (MQ=60)
                                              GAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGT                                                  <  SRR4302534.5277517/94‑1 (MQ=60)
                                              GAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGT                                                  <  SRR4302534.5037762/94‑1 (MQ=60)
                                                AATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGC                                                <  SRR4302534.971400/94‑1 (MQ=60)
                                                 ATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCT                                               <  SRR4302534.5105017/94‑1 (MQ=60)
                                                 ATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCT                                               >  SRR4302534.1160248/1‑94 (MQ=60)
                                                 ATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCT                                               <  SRR4302534.5220222/94‑1 (MQ=60)
                                                  TAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTC                                              >  SRR4302534.5365764/1‑94 (MQ=60)
                                                   ACCATCCTGTTGCTGTTCTCGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCG                                             <  SRR4302534.3668650/94‑1 (MQ=60)
                                                   AACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCG                                             <  SRR4302534.5289000/94‑1 (MQ=60)
                                                     CATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGAT                                           >  SRR4302534.1619679/1‑94 (MQ=60)
                                                        CCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGC                                        <  SRR4302534.597764/94‑1 (MQ=60)
                                                         CTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCA                                       >  SRR4302534.5819402/1‑94 (MQ=60)
                                                         CTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCA                                       <  SRR4302534.6122076/94‑1 (MQ=60)
                                                            TTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCA                                    <  SRR4302534.6102467/94‑1 (MQ=60)
                                                             TGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCA                                   >  SRR4302534.5586774/1‑94 (MQ=60)
                                                             TGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCA                                   >  SRR4302534.6404809/1‑94 (MQ=60)
                                                             TGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCA                                   <  SRR4302534.5813633/94‑1 (MQ=60)
                                                             TGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCA                                   <  SRR4302534.615864/94‑1 (MQ=60)
                                                              GCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAG                                  >  SRR4302534.3551571/1‑94 (MQ=60)
                                                              GCTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAG                                  <  SRR4302534.1400204/94‑1 (MQ=60)
                                                               CTGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGA                                 >  SRR4302534.3162812/1‑94 (MQ=60)
                                                                TGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCA                                <  SRR4302534.4148359/94‑1 (MQ=60)
                                                                TGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCA                                >  SRR4302534.6627341/1‑94 (MQ=60)
                                                                TGTTCACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCA                                <  SRR4302534.1602609/94‑1 (MQ=60)
                                                                 GTTCACGGGTCAGTGCGTTGAAGCGGAC‑GTCGTAGCCAGAGACGCGGAGTGTCAGGATAGGATATTGTTCCGGGGGCTCGCTGGCATCCAGCAG                               >  SRR4302534.3158441/1‑94 (MQ=57)
                                                                    CACGTGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCAT                            <  SRR4302534.904560/94‑1 (MQ=60)
                                                                    CACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCAT                            >  SRR4302534.1568450/1‑94 (MQ=60)
                                                                     ACGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATT                           <  SRR4302534.318881/94‑1 (MQ=60)
                                                                      CGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCCTTT                          >  SRR4302534.1916360/1‑94 (MQ=60)
                                                                      CGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTT                          <  SRR4302534.4060527/94‑1 (MQ=60)
                                                                      CGGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTT                          <  SRR4302534.1800559/94‑1 (MQ=60)
                                                                       GGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTGGATGGCATCCAGCAGCATTTC                         <  SRR4302534.2860122/94‑1 (MQ=60)
                                                                       GGGTCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTC                         >  SRR4302534.542518/1‑94 (MQ=60)
                                                                       GCGTCAGTGCGGTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTC                         <  SRR4302534.1414371/94‑1 (MQ=60)
                                                                          TCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCG                      <  SRR4302534.2673972/94‑1 (MQ=60)
                                                                          TCAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCG                      <  SRR4302534.2000871/94‑1 (MQ=60)
                                                                           CAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGA                     >  SRR4302534.4596194/1‑94 (MQ=60)
                                                                           CAGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGA                     >  SRR4302534.2864927/1‑94 (MQ=60)
                                                                            agtcctgTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCAGCCAGCAGCATTTCCCGAT                    <  SRR4302534.883525/87‑1 (MQ=60)
                                                                            AGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGATACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGAT                    >  SRR4302534.5172057/1‑94 (MQ=60)
                                                                            AGTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGAT                    >  SRR4302534.744981/1‑94 (MQ=60)
                                                                             GTGCGTTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATT                   <  SRR4302534.4050624/94‑1 (MQ=60)
                                                                                  TTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTA              <  SRR4302534.5942024/94‑1 (MQ=60)
                                                                                  TTGAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTA              <  SRR4302534.2130331/94‑1 (MQ=60)
                                                                                    GAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACG            <  SRR4302534.4002895/94‑1 (MQ=60)
                                                                                    GAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACG            <  SRR4302534.6615209/94‑1 (MQ=60)
                                                                                    GAAGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACG            >  SRR4302534.4014973/1‑94 (MQ=60)
                                                                                      AGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTT          >  SRR4302534.832854/1‑94 (MQ=60)
                                                                                      AGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTT          <  SRR4302534.1898585/94‑1 (MQ=60)
                                                                                      AGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTT          <  SRR4302534.5600682/94‑1 (MQ=60)
                                                                                      AGCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGGTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTT          >  SRR4302534.1052106/1‑94 (MQ=60)
                                                                                       GCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTG         <  SRR4302534.3773962/94‑1 (MQ=60)
                                                                                       GCGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTG         <  SRR4302534.5639171/94‑1 (MQ=60)
                                                                                        CGCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGA        >  SRR4302534.2640476/1‑94 (MQ=60)
                                                                                         GCACGG‑CGTAGCCAGAGATACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGAC       <  SRR4302534.1491166/94‑1 (MQ=60)
                                                                                         GCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGAC       >  SRR4302534.5035662/1‑94 (MQ=60)
                                                                                         GCACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGAC       <  SRR4302534.52875/94‑1 (MQ=60)
                                                                                          gacgG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACG      <  SRR4302534.913130/90‑1 (MQ=60)
                                                                                          CACGG‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACG      <  SRR4302534.2482739/94‑1 (MQ=60)
                                                                                            G‑G‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTG   <  SRR4302534.1008049/94‑1 (MQ=60)
                                                                                              G‑CGTAGCCAGAGACGCGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGA  <  SRR4302534.1470526/94‑1 (MQ=60)
                                                                                              |                                                                                              
TTTTTCATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCAC‑G‑CGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGA  >  NC_000913/3257745‑3257931

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑