Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 884 257.2 12958910 96.9% 12557183 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,432,762 T→C R304R (CGT→CGC)  fmt → 10‑formyltetrahydrofolate:L‑methionyl‑tRNA(fMet) N‑formyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,432,7620TC99.6% 859.7 / NA 249R304R (CGT→CGC) fmt10‑formyltetrahydrofolate:L‑methionyl‑tRNA(fMet) N‑formyltransferase
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (86/162);  total (87/162)
Fisher's exact test for biased strand distribution p-value = 3.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 257 total aligned reads displayed.

GGTTGCGACTGGTGATGGCATCCTGAACCTGCTCTCGTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGTCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCACTCTTCTAAGCCCGGTCTTGCCGGGCGTTTTTATACTTATGAAAAA  >  NC_000913/3432672‑3432851
                                                                                          |                                                                                         
 gTTGCGACTGGTGATGGCATCCTGAACCTGCTCTCGTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCgg                                                                                        <  1:1369565/93‑1 (MQ=255)
 gTTGCGACTGGTGATGGCATCCTGAACCTGCTCTCGTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCgg                                                                                        <  2:1683194/93‑1 (MQ=255)
  ttGCGACTGGTGATGGCATCCTGAACCTGCTCTCGTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCggg                                                                                       >  1:3967282/1‑93 (MQ=255)
   tGCGACTGGTGATGGCATCCTGAACCTGCTCTCGTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGa                                                                                      >  2:4025020/1‑93 (MQ=255)
    gCGACTGGTGATGGCATCCTGAACCTGCTCTCGTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGaa                                                                                     >  2:4477271/1‑93 (MQ=255)
     cGACTGGTGATGGCATCCTGAACCTGCTCTCGTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAAt                                                                                    <  1:1043926/93‑1 (MQ=255)
      gACTGGCGATGGCATCCTGAACCTGCTCTCGTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGCATg                                                                                   >  1:1581443/1‑93 (MQ=255)
       aCTGGTGATGGCATCCTGAACCTGCTCTCGTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATgg                                                                                  <  1:4021050/93‑1 (MQ=255)
        cTGGTGATGCCATCCTGAACCTGCTCTCGTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGt                                                                                 <  1:5523094/93‑1 (MQ=255)
        cTGGTGATGACATCCTGAACCTGCTCTCGTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGt                                                                                 <  1:1812224/93‑1 (MQ=255)
          ggTGATGGCATCCTGAACCTGCTCTCGTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGttt                                                                               >  2:1886013/1‑93 (MQ=255)
            tGATGGCATCCTGAACCTGCTCTCGTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGt                                                                             <  2:3897570/93‑1 (MQ=255)
            tGATGGCATCCTGAACCTGCTCTCGTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGt                                                                             <  2:43871/93‑1 (MQ=255)
            tGATGGCATCCTGAACCTGCTCTCGTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGt                                                                             >  2:2506858/1‑93 (MQ=255)
             gATGGCATCCTGAACCTGCTCTCGTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGtt                                                                            >  2:754072/1‑93 (MQ=255)
             gATGGCATCCTGAACCTGCTCTCGTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGtt                                                                            <  2:3297718/93‑1 (MQ=255)
              aTGGCATCCTGAACCTGCTCTCGTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTc                                                                           <  1:1746152/93‑1 (MQ=255)
               tGGCATCCTGAACCTGCTCTCGTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTcc                                                                          <  2:3664112/93‑1 (MQ=255)
                 gCATCCTGAACCTGCTCTCGTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCgg                                                                        >  1:748688/1‑93 (MQ=255)
                 gCATCCTGAACCTGCTCTCGTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCgg                                                                        <  1:3716977/93‑1 (MQ=255)
                    tCCTGAACCTGCTCTCGTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCa                                                                     <  2:5815177/93‑1 (MQ=255)
                    tCCTGAACCTGCTCTCGTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCa                                                                     >  1:3257285/1‑93 (MQ=255)
                    tCCTGAACCTGCTCTCGTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCa                                                                     >  1:959559/1‑93 (MQ=255)
                    tCCTGAACCTGCTCTCGTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCa                                                                     <  1:2601494/93‑1 (MQ=255)
                    tCCTGAACCTGCACTCGTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCa                                                                     >  1:1936629/1‑93 (MQ=255)
                      cTGAACCTGCTCTCGTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAAc                                                                   <  1:6252692/93‑1 (MQ=255)
                      cTGAACCTGCTCTCGTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAAc                                                                   <  2:3614492/93‑1 (MQ=255)
                      cTGAACCTGCTCTCGTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTCGTTCCGGGCAAc                                                                   <  2:3462139/93‑1 (MQ=255)
                       tGAACCTGCTCTCGTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAAcc                                                                  >  1:3241583/1‑93 (MQ=255)
                       tGAACCTGCTCTCGTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAAcc                                                                  >  1:5278345/1‑93 (MQ=255)
                        gAACCTGCTCTCGTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCg                                                                 <  2:2343730/93‑1 (MQ=255)
                         aaCCTGCTCTCGTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCgt                                                                <  2:2200777/93‑1 (MQ=255)
                         aaCCTGCTCTCGTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCgt                                                                <  1:696898/93‑1 (MQ=255)
                          aCCTGCTCTCGTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCgtc                                                               >  2:5078777/1‑93 (MQ=255)
                          aCCTGCTCTCGTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCgtc                                                               <  2:4608397/93‑1 (MQ=255)
                           ccTGCTCTCGTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCgtct                                                              >  2:4761276/1‑93 (MQ=255)
                             tGCTCTCGTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCgtctgg                                                            <  2:144485/93‑1 (MQ=255)
                             tGCTCTCGTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCgtctgg                                                            <  1:5531329/93‑1 (MQ=255)
                              gCTCTCGTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCgtctggt                                                           <  1:5925437/93‑1 (MQ=255)
                               ctctCGTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCgtctggtc                                                          >  1:5766305/1‑93 (MQ=255)
                                 ctcGTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGTAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCgtctggtctg                                                        <  2:1770962/93‑1 (MQ=255)
                                  tcGTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGa                                                       >  2:1906728/1‑93 (MQ=255)
                                  tcGTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGa                                                       >  1:4125383/1‑93 (MQ=255)
                                   cGTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGAt                                                      <  1:1195163/93‑1 (MQ=255)
                                   cGTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGAt                                                      <  2:6152052/93‑1 (MQ=255)
                                    gTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATa                                                     <  2:569711/93‑1 (MQ=255)
                                     ttACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAg                                                    >  1:419914/1‑93 (MQ=255)
                                      tACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGt                                                   <  2:406019/93‑1 (MQ=255)
                                      tACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGt                                                   <  1:828573/93‑1 (MQ=255)
                                       aCAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTc                                                  >  2:4136108/1‑93 (MQ=255)
                                        cAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTcc                                                 <  1:5692800/93‑1 (MQ=255)
                                          aCCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCAc                                               >  1:2457269/1‑93 (MQ=255)
                                          aCCTGCGGATAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCAc                                               <  1:5267052/93‑1 (MQ=255)
                                            cTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCActc                                             <  1:2908224/93‑1 (MQ=255)
                                            cTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCActc                                             >  1:3001768/1‑93 (MQ=255)
                                            cTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCActc                                             <  1:6470218/93‑1 (MQ=255)
                                             tGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCActct                                            <  1:4343758/93‑1 (MQ=255)
                                             tGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCActct                                            <  1:25071/93‑1 (MQ=255)
                                               cGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCACtcttc                                          >  2:1923145/1‑93 (MQ=255)
                                                gggTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCACtcttct                                         <  1:5050125/93‑1 (MQ=255)
                                                gggTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCACtcttct                                         >  1:209133/1‑93 (MQ=255)
                                                gggTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCACtcttct                                         <  2:1705675/93‑1 (MQ=255)
                                                gggTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCACtcttct                                         >  2:209795/1‑93 (MQ=255)
                                                 ggTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCACTCTTCTa                                        >  2:337507/1‑93 (MQ=255)
                                                 ggTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCACTCTTCTa                                        <  2:1032706/93‑1 (MQ=255)
                                                     aGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCACTCTTCTAAGcc                                    >  1:3898973/1‑93 (MQ=255)
                                                     aGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCACTCTTCTAAGcc                                    <  2:12597/93‑1 (MQ=255)
                                                     aGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCACTCTTCTAAGcc                                    >  2:6275289/1‑93 (MQ=255)
                                                      gAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCATCCGTCTGGTCTGATAGTCCACTCTTCTAAGccc                                   <  2:5333352/93‑1 (MQ=255)
                                                      gAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCACTCTTCTAAGccc                                   <  2:4894884/93‑1 (MQ=255)
                                                      gAAAGCGATGAGCGCGAAAGCCCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCACTCTTCTAAGccc                                   <  2:1967608/93‑1 (MQ=255)
                                                        aaGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCACTCTTCTAAGCCCgg                                 >  2:2667043/1‑93 (MQ=255)
                                                          gCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCACTCTTCTAAGCCCGGTc                               <  1:5831632/93‑1 (MQ=255)
                                                          gCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCACTCTTCTAAGCCCGGTc                               <  1:4254203/93‑1 (MQ=255)
                                                          gCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCACTCTTCTAAGCCCGGTc                               <  1:5346712/93‑1 (MQ=255)
                                                           cGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCACTCTTCTAAGCCCGGTCt                              <  1:4485934/93‑1 (MQ=255)
                                                           cGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCACTCTTCTAAGCCCGGTCt                              <  2:1303978/93‑1 (MQ=255)
                                                            gATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCACTCTTCTAAGCCCGGTCtt                             >  2:5204679/1‑93 (MQ=255)
                                                            gATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCACTCTTCTAAGCCCGGTCtt                             <  2:860807/93‑1 (MQ=255)
                                                            gATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCACTCTTCTAAGCCCGGTCtt                             >  2:3806930/1‑93 (MQ=255)
                                                              tGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCACTCTTCTAAGCCCGGTCTTGc                           <  2:2605289/93‑1 (MQ=255)
                                                              tGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCACTCTTCTAAGCCCGGTCTTGc                           >  1:6333154/1‑93 (MQ=255)
                                                               gAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCACTCTTCTAAGCCCGGTCTTGcc                          <  2:3109695/93‑1 (MQ=255)
                                                               gAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCACTCTTCTAAGCCCGGTCTTGcc                          <  1:3474309/93‑1 (MQ=255)
                                                                   gcgcAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCACTCTTCTAAGCCCGGTCTTGCCGGGc                      <  2:6431388/93‑1 (MQ=255)
                                                                   gcgcAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCACTCTTCTAAGCCCGGTCTTGCCGGGc                      <  1:3793704/93‑1 (MQ=255)
                                                                     gcAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCACTCTTCTAAGCCCGGTCTTGCCGGGCGt                    <  1:6056703/93‑1 (MQ=255)
                                                                      cAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCACTCTTCTAAGCCCGGTCTTGCCGGGCGtt                   <  2:4346559/93‑1 (MQ=255)
                                                                      cAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCACTCTTCTAAGCCCGGTCTTGCCGGGCGtt                   <  1:5058893/93‑1 (MQ=255)
                                                                      cAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCACTCTTCTAAGCCCGGTCTTGCCGGGCGtt                   <  1:2707017/93‑1 (MQ=255)
                                                                       aaGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCACTCTTCTAAGCCCGGTCTTGCCGGGCGttt                  <  2:5473683/93‑1 (MQ=255)
                                                                        aGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCACTCTTCTAAGCCCGGTCTTGCCGGGCGtttt                 >  1:1439357/1‑93 (MQ=255)
                                                                        aGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCACTCTTCTAAGCCCGGTCTTGCCGGGCGtttt                 <  1:3146353/93‑1 (MQ=255)
                                                                        aGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCACTCTTCTAAGCCCGGTCTTGCCGGGCGtttt                 >  2:5269742/1‑93 (MQ=255)
                                                                         gACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCACTCTTCTAAGCCCGGTCTTGCCGGGCGttttt                <  2:2800371/93‑1 (MQ=255)
                                                                              cctGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCACTCTTCTAAGCCCGGTCTTGCCGGGCGTTTTTATACt           >  1:4761087/1‑93 (MQ=255)
                                                                               ctGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCACTCTTCTAAGCCCGGTCTTGCCGGGCGTTTTTATACtt          <  1:927396/93‑1 (MQ=255)
                                                                                   aCTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCACTCTTCTAAGCCCGGTCTTGCCGGGCGTTTTTATACTTATGa      >  2:5919369/1‑93 (MQ=255)
                                                                                   aCTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCACTCTTCTAAGCCCGGTCTTGCCGGGCGTTTTTATACTTATGa      <  1:1716649/93‑1 (MQ=255)
                                                                                     tctcGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCACTCTTCTAAGCCCGGTCTTGCCGGGCGTTTTTATACTTATGaaa    >  1:2046501/1‑93 (MQ=255)
                                                                                          |                                                                                         
GGTTGCGACTGGTGATGGCATCCTGAACCTGCTCTCGTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGTCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCACTCTTCTAAGCCCGGTCTTGCCGGGCGTTTTTATACTTATGAAAAA  >  NC_000913/3432672‑3432851

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGGTTGCGACTGGTGATGGCATCCTGAACCTGCTCTCGTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGTCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCACTCTTCTAAGCCCGGTCTTGCCGGGCGTTTTTATACTTATGAAAAAA  >  NC_000913/3432671‑3432852
                                                                                           |                                                                                          
AGTTTGCGACTGGTGATGGCATCCTGAACCTGCTCTCGTTACAACCTGCGTGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCG                                                                                          <  SRR4302534.2328571/94‑1 (MQ=60)
 GGTTGCGACTGGTGATGGCATCCTGAACCTGCTCTCGTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGG                                                                                         <  SRR4302534.5190108/94‑1 (MQ=60)
 GGTTGCGACTGGTGATGGCATCCTGAACCTGCTCTCGTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGG                                                                                         <  SRR4302534.958328/94‑1 (MQ=60)
  GTTGCGACTGGTGATGGCATCCTGAACCTGCTCTCGTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGG                                                                                        >  SRR4302534.4073479/1‑94 (MQ=60)
  GTTGCGACTGGTGATGGCATCCTGAACCTGCTCTCGTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGG                                                                                        <  SRR4302534.1405651/94‑1 (MQ=60)
  GTCGCGACTGCTGATGGCATCCTGACCCTGCTCTCGTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACGACCTGAACTCTCGCCGGG                                                                                        <  SRR4302534.3611379/94‑1 (MQ=60)
      CGACTGGTGATGGCATCCTGAACCTGCTCTCGTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATG                                                                                    <  SRR4302534.6262557/94‑1 (MQ=60)
      CGACTGGTGATGGCATCCTGAACCTGCTCTCGTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATG                                                                                    <  SRR4302534.1070888/94‑1 (MQ=60)
      CGACTGGTGATGGCATCCTGAACCTGCTCTCGTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATG                                                                                    >  SRR4302534.3902602/1‑94 (MQ=60)
      CGACTGGCGATGGCATCCTGAACCTGCTCTCGTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGCATG                                                                                    >  SRR4302534.1622805/1‑94 (MQ=60)
        ACTGGTGATGGCATCCTGAACCTGCTCTCGTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGT                                                                                  <  SRR4302534.4128853/94‑1 (MQ=60)
        ACTGGTGATGGCATCCTGAACCTGCTCTCGTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGT                                                                                  <  SRR4302534.5447108/94‑1 (MQ=60)
        ACTGGTGATGCCAT‑CTTAACCTGCTCTCGTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTT                                                                                 <  SRR4302534.2108250/94‑1 (MQ=60)
         CTGGTGATGGCATCCTGAACCTGCTCTCGTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTT                                                                                 <  SRR4302534.5222519/94‑1 (MQ=60)
         CTGGTGATGCCATCCTGAACCTGCTCTCGTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTT                                                                                 <  SRR4302534.5672137/94‑1 (MQ=60)
         CTGGTGATGACATCCTGAACCTGCTCTCGTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTT                                                                                 <  SRR4302534.1859293/94‑1 (MQ=60)
           GGTGATGGCATCCTGAACCTGCTCTCGTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTG                                                                               <  SRR4302534.1790846/94‑1 (MQ=60)
             TGATGGCATCCTGAACCTGCTCTCGTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTT                                                                             <  SRR4302534.5970560/94‑1 (MQ=60)
              GATGGCATCCTGAACCTGCTCTCGTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTAGCCGGGAATGGTTTGTTC                                                                            >  SRR4302534.2065951/1‑94 (MQ=60)
               ATGGCATCCTGAACCTGCTCTCGTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCC                                                                           <  SRR4302534.1791461/94‑1 (MQ=60)
                TGGCATCCTGAACCTGCTCTCGTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCG                                                                          >  SRR4302534.9205/1‑94 (MQ=60)
                TGGCATCCTGAACCTGCTCTCGTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCG                                                                          <  SRR4302534.1379407/94‑1 (MQ=60)
                 GGCATCCTGAACCTGCTCTCGTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCTG                                                                         >  SRR4302534.3415918/1‑94 (MQ=60)
                 GGCATCCTGAACCTGCTCTCGTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGG                                                                         >  SRR4302534.767241/1‑94 (MQ=60)
                 GGCATCCTGAACCTGCTCTCGTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGG                                                                         >  SRR4302534.2565847/1‑94 (MQ=60)
                  GCATCCTGAACCTGCTCTCGTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGG                                                                        <  SRR4302534.441727/94‑1 (MQ=60)
                  GCATCCTGAACCTGCTCTCGTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGG                                                                        <  SRR4302534.5943891/94‑1 (MQ=60)
                  GCATCCTGAACCTGCTCTCGTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGG                                                                        <  SRR4302534.3816908/94‑1 (MQ=60)
                   CATCCTGAACCTGCTCTCGTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGC                                                                       >  SRR4302534.5672438/1‑94 (MQ=60)
                    ATCCTGAACCTGCTCTCGTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCA                                                                      >  SRR4302534.3344030/1‑94 (MQ=60)
                    ATCCTGAACCTGCTCTCGTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCA                                                                      >  SRR4302534.984007/1‑94 (MQ=60)
                    ATCCTGAACCTGCACTCGTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCA                                                                      >  SRR4302534.1987263/1‑94 (MQ=60)
                     TCCTGAACCTGCTCTCGTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAA                                                                     <  SRR4302534.2670318/94‑1 (MQ=60)
                     TCCTGAACCTGCTCTCGTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAA                                                                     <  SRR4302534.4117165/94‑1 (MQ=60)
                       CTGAACCTGCTCTCGTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACC                                                                   >  SRR4302534.5420869/1‑94 (MQ=60)
                       CTGAACCTGCTCTCGTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACC                                                                   <  SRR4302534.2622292/94‑1 (MQ=60)
                       CTGAACCTGCTCTCGTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACC                                                                   >  SRR4302534.5763110/1‑94 (MQ=60)
                       CTGAACCTGCTCTCGTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACC                                                                   >  SRR4302534.3327837/1‑94 (MQ=60)
                       CTGAACCTGCTCTCGTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACC                                                                   <  SRR4302534.6422198/94‑1 (MQ=60)
                          AACCTGCTCTCGTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTC                                                                >  SRR4302534.54574/1‑94 (MQ=60)
                          AACCTGCTCTCGTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTC                                                                <  SRR4302534.713967/94‑1 (MQ=60)
                           ACCTGCTCTCTTTACAACCTGCGGATAAGAAAGCGATGAGCGCGCAAGTCCTCCTGAGCTCTCGCCGGGAATGGTTTGTGCCGGGCAACCGTCT                                                               <  SRR4302534.4455327/94‑1 (MQ=60)
                            CCTGCTCTCGTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTG                                                              <  SRR4302534.3883035/94‑1 (MQ=60)
                              TGCTCTCGTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGT                                                            <  SRR4302534.5680631/94‑1 (MQ=60)
                               GCTCTCGTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAATTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTC                                                           >  SRR4302534.3436212/1‑94 (MQ=60)
                               GCTCTCGTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTC                                                           <  SRR4302534.6086336/94‑1 (MQ=60)
                               GCTCTCGTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTC                                                           >  SRR4302534.5922742/1‑94 (MQ=60)
                               GCTCTCGTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTC                                                           <  SRR4302534.5257707/94‑1 (MQ=60)
                                 TCTCGTTACACCCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTG                                                         <  SRR4302534.964603/94‑1 (MQ=60)
                                 TCTCGTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGCCCTCCTGACCTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTG                                                         <  SRR4302534.3963155/94‑1 (MQ=60)
                                 TCTCGTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTG                                                         >  SRR4302534.190106/1‑94 (MQ=60)
                                  CTCGTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGA                                                        >  SRR4302534.4236435/1‑94 (MQ=60)
                                    TGTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATA                                                      <  SRR4302534.4113329/94‑1 (MQ=60)
                                    CGTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATA                                                      <  SRR4302534.1226607/94‑1 (MQ=60)
                                     GTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAG                                                     >  SRR4302534.430654/1‑94 (MQ=60)
                                       TACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTC                                                   <  SRR4302534.849425/94‑1 (MQ=60)
                                       TACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTC                                                   <  SRR4302534.6625683/94‑1 (MQ=60)
                                        ACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCC                                                  <  SRR4302534.6625852/94‑1 (MQ=60)
                                        ACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCC                                                  <  SRR4302534.3075233/94‑1 (MQ=60)
                                         CAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCA                                                 <  SRR4302534.3343312/94‑1 (MQ=60)
                                         CAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCA                                                 <  SRR4302534.5846991/94‑1 (MQ=60)
                                         CAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCA                                                 <  SRR4302534.5078630/94‑1 (MQ=60)
                                         CAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCA                                                 >  SRR4302534.4576761/1‑94 (MQ=60)
                                         CAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCA                                                 <  SRR4302534.3662419/94‑1 (MQ=60)
                                          AACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCAC                                                >  SRR4302534.2522554/1‑94 (MQ=60)
                                           ACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGTGCAACCGTCTGGTCTGATAGTCCACT                                               >  SRR4302534.2493125/1‑94 (MQ=60)
                                           ACCTGCGGATAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCACT                                               <  SRR4302534.5409329/94‑1 (MQ=60)
                                            CCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCACTC                                              >  SRR4302534.3080286/1‑94 (MQ=60)
                                             CTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCACTCT                                             <  SRR4302534.6644496/94‑1 (MQ=60)
                                             CTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCACTCT                                             <  SRR4302534.73098/94‑1 (MQ=60)
                                             CTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCACTCT                                             <  SRR4302534.2984559/94‑1 (MQ=60)
                                              TGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCACTCTT                                            <  SRR4302534.25730/94‑1 (MQ=60)
                                              TGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCACTCTT                                            <  SRR4302534.4461088/94‑1 (MQ=60)
                                              TGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCACTCTT                                            >  SRR4302534.6426841/1‑94 (MQ=60)
                                               GCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCACTCTTC                                           <  SRR4302534.1507348/94‑1 (MQ=60)
                                                CGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCACTCTTCT                                          <  SRR4302534.6170900/94‑1 (MQ=60)
                                                CGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCACTCTTCT                                          >  SRR4302534.214628/1‑94 (MQ=60)
                                                 GGGTAAGAAATCGATGAGCACGCAAGAGCACCTTAACACTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCTCTCTTCTA                                         <  SRR4302534.6524926/94‑1 (MQ=60)
                                                 GGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCACTCTTCTA                                         <  SRR4302534.5186488/94‑1 (MQ=60)
                                                  GGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCACTCTTCTAA                                        <  SRR4302534.1639639/94‑1 (MQ=60)
                                                   GTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCACTCTTCTAAG                                       <  SRR4302534.5499139/94‑1 (MQ=60)
                                                     AAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCACTCTTCTAAGCC                                     >  SRR4302534.4003590/1‑94 (MQ=60)
                                                     AAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTACACTCTTCTAAGCC                                     >  SRR4302534.3072279/1‑94 (MQ=60)
                                                       GAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCACTCTTCTAAGCCCG                                   <  SRR4302534.2803788/94‑1 (MQ=60)
                                                       GAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCACTCTTCTAAGCCCG                                   >  SRR4302534.4403394/1‑94 (MQ=60)
                                                       GAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCACTCTTCTAAGCCCG                                   <  SRR4302534.709624/94‑1 (MQ=60)
                                                        AAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCACTCTTCTAAGCCCGG                                  >  SRR4302534.4331711/1‑94 (MQ=60)
                                                        AAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCACTCTTCTAAGCCCGG                                  <  SRR4302534.75242/94‑1 (MQ=60)
                                                          AGCGATGAGCCCGCACGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCACCCGTCTGGTCTTATAGTCCACTCTTCTAAGCCCGGTC                                <  SRR4302534.4532351/94‑1 (MQ=60)
                                                           GCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCACTCTTCTAAGCCCGGTCT                               <  SRR4302534.5490874/94‑1 (MQ=60)
                                                           GCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCACTCTTCTAAGCCCGGTCT                               <  SRR4302534.5989928/94‑1 (MQ=60)
                                                           GCGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCACTCTTCTAAGCCCGGTCT                               <  SRR4302534.4368999/94‑1 (MQ=60)
                                                            CGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCACTCTTCTAAGCCCGGTCTT                              <  SRR4302534.1769886/94‑1 (MQ=60)
                                                            CGATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCACTCTTCTAAGCCCGGTCTT                              <  SRR4302534.4607311/94‑1 (MQ=60)
                                                             GATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCACTCTTCTAAGCCCGGTCTTG                             >  SRR4302534.1215575/1‑94 (MQ=60)
                                                              ATGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCACTCTTCTAAGCCCGGTCTTGC                            >  SRR4302534.6504246/1‑94 (MQ=60)
                                                               TGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCACTCTTCTAAGCCCGGTCTTGCC                           <  SRR4302534.2740009/94‑1 (MQ=60)
                                                               TGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCACTCTTCTAAGCCCGGTCTTGCC                           <  SRR4302534.4742166/94‑1 (MQ=60)
                                                               TGAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCACTCTTCTAAGCCCGGTCTTGCC                           <  SRR4302534.1909591/94‑1 (MQ=60)
                                                                GAGCGCTCAAGACCTCCTGACCTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCACTCTTCTAAGCCCGGTCTTGCCG                          <  SRR4302534.4012516/94‑1 (MQ=60)
                                                                GAGCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCACTCTTCTAAGCCCGGTCTTGCCG                          <  SRR4302534.3567698/94‑1 (MQ=60)
                                                                  GCGCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCACTCTTCTAAGCCCGGTCTTGCCGGG                        <  SRR4302534.832575/94‑1 (MQ=60)
                                                                    GCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCACTCTTCTAAGCCCGGTCTTGCCGGGCG                      <  SRR4302534.6292571/94‑1 (MQ=60)
                                                                    GCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCACTCTTCTAAGCCCGGTCTTGCCGGGCG                      <  SRR4302534.3895551/94‑1 (MQ=60)
                                                                    GCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCACTCTTCTAAGCCCGGTCTTGCCGGGCG                      <  SRR4302534.4132487/94‑1 (MQ=60)
                                                                    GCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCACTCTTCTAAGCCCGGTCTTGCCGGGCG                      >  SRR4302534.4249133/1‑94 (MQ=60)
                                                                    GCGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCACTCTTCTAAGCCCGGTCTTGCCGGGCG                      >  SRR4302534.2092222/1‑94 (MQ=60)
                                                                     CGCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCACTCTTCTAAGCCCGGTCTTGCCGGGCGT                     <  SRR4302534.3301717/94‑1 (MQ=60)
                                                                      GCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCACTCTTCTAAGCCCGGTCTTGCCGGGCGTT                    <  SRR4302534.5850604/94‑1 (MQ=60)
                                                                      GCAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCACTCTTCTAAGCCCGGTCTTGCCGGGCGTT                    <  SRR4302534.6221405/94‑1 (MQ=60)
                                                                       CAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCACTCTTCTAAGCCCGGTCTTGCCGGGCGTTT                   <  SRR4302534.2778502/94‑1 (MQ=60)
                                                                       CAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCACTCTTCTAAGCCCGGTCTTGCCGGGCGTTT                   <  SRR4302534.5691352/94‑1 (MQ=60)
                                                                       CAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCACTCTTCTAAGCCCGGTCTTGCCGGGCGTTT                   <  SRR4302534.5195528/94‑1 (MQ=60)
                                                                       CAAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCACTCTTCTAAGCCCGGTCTTGCCGGGCGTTT                   <  SRR4302534.2509757/94‑1 (MQ=60)
                                                                        AAGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCACTCTTCTAAGCCCGGTCTTGCCGGGCGTTTT                  >  SRR4302534.1477247/1‑94 (MQ=60)
                                                                         AGACCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCACTCTTCTAAGCCCGGTCTTGCCGGGCGTTTTT                 <  SRR4302534.3229107/94‑1 (MQ=60)
                                                                            CCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCACTCTTCTAAGCCCGGTCTTGCCGGGCGTTTTTATA              <  SRR4302534.2800847/94‑1 (MQ=60)
                                                                            CCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCACTCTTCTAAGCCCGGTCTTGCCGGGCGTTTTTATA              >  SRR4302534.4255073/1‑94 (MQ=60)
                                                                            CCTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCACTCTTCTAAGCCCGGTCTTGCCGGGCGTTTTTATA              <  SRR4302534.3858112/94‑1 (MQ=60)
                                                                             CTCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCACTCTTCTAAGCCCGGTCTTGCCGGGCGGTTTTATAC             >  SRR4302534.1270447/1‑94 (MQ=60)
                                                                              TCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCACTCTTCTAAGCCCGGTCTTGCCGGGCGTTTTTATACT            >  SRR4302534.4890363/1‑94 (MQ=60)
                                                                              TCCTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCACTCTTCTAAGCCCGGTCTTGCCGGGCGTTTTTATACT            <  SRR4302534.1534240/94‑1 (MQ=60)
                                                                              TCCTGAACTCTCGCCGGGAATGGTTTGCTCCGGGCAACCGTCTGGTCTGATAGTCCACTCTTCTAAGACCGGTCTTGCCGGGCGTTTTTATACT            >  SRR4302534.1828971/1‑94 (MQ=60)
                                                                                CTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCACTCTTCTAAGCCCGGTCTTGCCGGGCGTTTTTATACTTA          <  SRR4302534.951005/94‑1 (MQ=60)
                                                                                CTGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCACTCTTCTAAGCCCGGTCTTGCCGGGCGTTTTTATACTTA          <  SRR4302534.904238/94‑1 (MQ=60)
                                                                                 TGAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCACTCTTCTAAGCCCGGTCTTGCCGGGCGTTTTTATACTTAT         <  SRR4302534.4908472/94‑1 (MQ=60)
                                                                                  GAACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCACTCTTCTAAGCCCGGTCTTGCCGGGCGTTTTTATACTTATG        <  SRR4302534.259756/94‑1 (MQ=60)
                                                                                   AACTCTCGCCGGGAATGGTTTGTTCCGGGCAGCCGTCTGGTCTGATAGTCCACTCTTCTAAGCCCGGTCTTGCCGGGCGTTTTTATACTTATGA       <  SRR4302534.1450447/94‑1 (MQ=60)
                                                                                    ACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCACTCTTCTAAGCCCGGTCTTGCCGGGCGTTTTTATACTTATGAA      >  SRR4302534.3300401/1‑94 (MQ=60)
                                                                                    ACTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCACTCTTCTAAGCCCGGTCTTGCCGGGCGTTTTTATACTTATGAA      <  SRR4302534.1761242/94‑1 (MQ=60)
                                                                                     CTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCACTCTTCTAAGCCCGGTCTTGCCGGGCGTTTTTATACTTATGAAA     <  SRR4302534.6201771/94‑1 (MQ=60)
                                                                                     CTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCACTCTTCTAAGCCCGGTCTTGCCGGGCGTTTTTATACTTATGAAA     <  SRR4302534.3132264/94‑1 (MQ=60)
                                                                                     CTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCACTCTTCTAAGCCCGGTCTTGCCGGGCGTTTTTATACTTATGAAA     >  SRR4302534.2099991/1‑94 (MQ=60)
                                                                                     CTCTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCACTCTTCTAAGCCCGGTCTTGCCGGGCGTTTTTATACTTATGAAA     >  SRR4302534.5415022/1‑94 (MQ=60)
                                                                                       CTCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCACTCTTCTAAGCCCGGTCTTGCCGGGCGTTTTTATACTTATGAAAAA   >  SRR4302534.5519671/1‑94 (MQ=60)
                                                                                        TCGCCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCACTCTTCTAAGCCCGGTCTTGCCGGGCGTTTTAATACTTATGAAAAAA  <  SRR4302534.6065504/94‑1 (MQ=60)
                                                                                           |                                                                                          
AGGTTGCGACTGGTGATGGCATCCTGAACCTGCTCTCGTTACAACCTGCGGGTAAGAAAGCGATGAGCGCGCAAGACCTCCTGAACTCTCGTCGGGAATGGTTTGTTCCGGGCAACCGTCTGGTCTGATAGTCCACTCTTCTAAGCCCGGTCTTGCCGGGCGTTTTTATACTTATGAAAAAA  >  NC_000913/3432671‑3432852

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑