Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	884	257.2	12958910	96.9%	12557183	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,446,647	T→C	T54A (ACC→GCC)	rplP ←	50S ribosomal subunit protein L16

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,446,647	0	T	C	99.6%	838.0 / NA	240	T54A (ACC→GCC)	rplP	50S ribosomal subunit protein L16
Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (132/107); total (133/107)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 244 total aligned reads displayed.

TACCCATACGCACTGCCAGCGGCTTTTCAGTGATCGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGTCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCG  >  NC_000913/3446557‑3446736

taccCATACGCACTGCCAGCGGCTTTTCAGTGATCGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCa                                                                                         >  2:1835120/1‑93 (MQ=255)

  cccATACGCACTGCCAGCGGCTTTTCAGTGATCGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATa                                                                                       <  2:5258373/93‑1 (MQ=255)

   ccATACGCACTGCCAGCGGCTTTTCAGTGATCGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGTCATAg                                                                                      >  1:274512/1‑93 (MQ=255)

   ccATACGCACTGCCAGCGGCTTTTCAGTGATCGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAg                                                                                      <  2:4230198/93‑1 (MQ=255)

   ccATACGCACTGCCAGCGGCTTTTCAGTGATCGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAg                                                                                      <  1:5884644/93‑1 (MQ=255)

   ccATACGCACTGCCAGCGGCTTTTCAGTGATCGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAg                                                                                      <  1:2304501/93‑1 (MQ=255)

    cATACGCACTGCCAGCGGCTTTTCAGTGATCGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGc                                                                                     <  1:4622702/93‑1 (MQ=255)

    cATACGCACTGCCAGCGGCTTTTCAGTGATCGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGc                                                                                     >  1:5582202/1‑93 (MQ=255)

    cATACGCACTGCCAGCGGCTTTTCAGTGATCGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGc                                                                                     >  1:182227/1‑93 (MQ=255)

      tACGCACTGCCAGCGGCTTTTCAGTGATCGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCac                                                                                   >  1:1465806/1‑93 (MQ=255)

      tACGCACTGCCAGCGGCTTTTCAGTGATCGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCac                                                                                   <  2:1573102/93‑1 (MQ=255)

        cGCCCTGCCAGCGGCTTTTCAGTGATCGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCacga                                                                                 <  1:5268996/93‑1 (MQ=255)

        cGCACTGCCAGCGGCTTTTCAGTGATCGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCacga                                                                                 >  2:272326/1‑93 (MQ=255)

        cGCACTGCCAGCGGCTTTTCAGTGATCGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCacga                                                                                 <  2:4829100/93‑1 (MQ=255)

         gCACTGCCAGCGGCTTTTCAGTGATCGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCacgac                                                                                <  1:1069659/93‑1 (MQ=255)

         gCACTGCCAGCGGCTTTTCAGTGATCGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCacgac                                                                                <  1:5053705/93‑1 (MQ=255)

         gCACTGCCAGCGGCTTTTCAGTGATCGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCacgac                                                                                <  2:4318935/93‑1 (MQ=255)

         gCACTGCCAGCGGCTTTTCAGTGATCGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCacgac                                                                                <  1:1612716/93‑1 (MQ=255)

          cACTGCCAGCGGCTTTTCAGTGATCGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCacgacg                                                                               <  2:2345650/93‑1 (MQ=255)

           aCTGCCAGCGGCTTTTCAGTGATCGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCACGACGt                                                                              >  1:923592/1‑93 (MQ=255)

             tGCCAGCGGCTTTTCAGTGATCGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCACGACGtgc                                                                            >  1:3072598/1‑93 (MQ=255)

             tGCCAGCGGCTTTTCAGTGATCGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCACGACGtgc                                                                            <  2:3358066/93‑1 (MQ=255)

              gCCAGCGGCTTTTCAGTGATCGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCACGACGtgct                                                                           <  2:2651326/93‑1 (MQ=255)

              gCCAGCGGCTTTTCAGTGATCGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCACGACGtgct                                                                           <  2:4046349/93‑1 (MQ=255)

                cAGCGGCTTTTCAGTGATCGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCACGACGtgctgc                                                                         >  1:587533/1‑93 (MQ=255)

                 aGCGGCTTTTCAGTGATCGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCACGACGTgctgct                                                                        >  1:326771/1‑93 (MQ=255)

                 aGCGGCTTTTCAGTGATCGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCACGACGTgctgct                                                                        <  1:3364193/93‑1 (MQ=255)

                  gCGGCTTTTCAGTGATCGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCtt                                                                       >  1:6414956/1‑93 (MQ=255)

                   cGGCTTTTCAGTGATCGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTc                                                                      <  1:6002374/93‑1 (MQ=255)

                     gCTTTTCAGTGATCGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGa                                                                    >  2:5079272/1‑93 (MQ=255)

                       ttttCAGTGATCGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATc                                                                  >  2:402282/1‑93 (MQ=255)

                        tttCAGTGATCGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCt                                                                 >  1:4993275/1‑93 (MQ=255)

                        tttCAGTGATCGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCt                                                                 >  2:1315295/1‑93 (MQ=255)

                         ttCAGTGATCGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTg                                                                <  2:628161/93‑1 (MQ=255)

                         ttCAGTGATCGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTg                                                                <  1:5792728/93‑1 (MQ=255)

                          tCAGTGATCGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGa                                                               <  2:1161695/93‑1 (MQ=255)

                           cAGTGATCGTTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGAc                                                              <  1:3607951/93‑1 (MQ=255)

                           cAGTGATCGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGAc                                                              >  1:5220591/1‑93 (MQ=255)

                           cAGTGATCGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGAc                                                              >  1:6079422/1‑93 (MQ=255)

                            aGTGATCGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGACg                                                             >  1:1116691/1‑93 (MQ=255)

                             gTGATCGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGACgg                                                            <  2:5340835/93‑1 (MQ=255)

                               gATCGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGc                                                          <  2:547280/93‑1 (MQ=255)

                               gATCGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGc                                                          <  1:2211446/93‑1 (MQ=255)

                                 tCGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAg                                                        >  1:874427/1‑93 (MQ=255)

                                      ttGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAga                                                   <  2:295474/93‑1 (MQ=255)

                                         tCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAgacga                                                >  2:571723/1‑93 (MQ=255)

                                         tCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAgacga                                                >  1:4408443/1‑93 (MQ=255)

                                             ggAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCAc                                            >  2:4490443/1‑93 (MQ=255)

                                              gAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACg                                           <  1:679985/93‑1 (MQ=255)

                                              gAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACg                                           >  2:4802264/1‑93 (MQ=255)

                                               aaCACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACgg                                          >  2:1465086/1‑93 (MQ=255)

                                               aaCACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACgg                                          <  2:4378457/93‑1 (MQ=255)

                                               aaCACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACgc                                          >  2:4209230/1‑92 (MQ=255)

                                                acacacGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGc                                         >  2:1923008/1‑93 (MQ=255)

                                                acacacGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGc                                         >  1:1503580/1‑93 (MQ=255)

                                                  acacGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCa                                       <  2:3733216/93‑1 (MQ=255)

                                                  acacGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCa                                       >  2:5243336/1‑93 (MQ=255)

                                                   cacGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGTCaa                                      >  1:4769111/1‑93 (MQ=255)

                                                    acGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAAc                                     >  2:4643274/1‑93 (MQ=255)

                                                       gATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATGTGACGGGCAGTCAGACGACCACGGCCAACAGc                                  <  1:5717445/93‑1 (MQ=255)

                                                        aTCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCt                                 >  2:65639/1‑93 (MQ=255)

                                                        aTCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCt                                 >  2:6277770/1‑93 (MQ=255)

                                                         tCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCtt                                >  2:5863210/1‑93 (MQ=255)

                                                           cAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTc                              >  2:2179484/1‑93 (MQ=255)

                                                            aGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCa                             <  2:3004696/93‑1 (MQ=255)

                                                            aGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCa                             <  2:3806396/93‑1 (MQ=255)

                                                             gATCTTACCTTGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAg                            >  2:3635624/1‑93 (MQ=255)

                                                              aTCTTACCTTGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGa                           <  2:2076978/93‑1 (MQ=255)

                                                                  tACCTTGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGa                       <  2:5029532/93‑1 (MQ=255)

                                                                  tACCTTGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGa                       <  1:1192985/93‑1 (MQ=255)

                                                                   aCCTTGACGCTTAACTGCACGGGCCATAGCACGTCGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGaa                      >  1:1962517/1‑93 (MQ=255)

                                                                   aCCTTGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGaa                      >  1:5705854/1‑93 (MQ=255)

                                                                    ccTTGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAg                     >  2:1084943/1‑93 (MQ=255)

                                                                     cTTGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGc                    >  1:4209255/1‑93 (MQ=255)

                                                                      ttGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCt                   >  2:732711/1‑93 (MQ=255)

                                                                      ttGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCt                   >  2:2313320/1‑93 (MQ=255)

                                                                       tGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTg                  >  1:1747433/1‑93 (MQ=255)

                                                                        gACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGc                 <  1:3781925/93‑1 (MQ=255)

                                                                        gACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGc                 >  1:4152719/1‑93 (MQ=255)

                                                                        gACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGc                 >  1:4462305/1‑93 (MQ=255)

                                                                         aCGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGcc                <  2:3063580/93‑1 (MQ=255)

                                                                            cTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGaa             <  1:2905347/93‑1 (MQ=255)

                                                                             ttAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAg            >  1:5106463/1‑93 (MQ=255)

                                                                              tAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGc           <  2:936568/93‑1 (MQ=255)

                                                                               aaCTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCt          >  1:2518623/1‑93 (MQ=255)

                                                                               aaCTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCt          <  2:802745/93‑1 (MQ=255)

                                                                                aCTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTa         >  2:2222000/1‑93 (MQ=255)

                                                                                aCTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTa         >  1:3090360/1‑93 (MQ=255)

                                                                                aCTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTa         >  2:3150913/1‑93 (MQ=255)

                                                                                 cTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTaa        <  2:3988412/93‑1 (MQ=255)

                                                                                   gCACGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACa      <  1:2403196/93‑1 (MQ=255)

                                                                                    cACGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACAt     <  1:4558163/93‑1 (MQ=255)

                                                                                    cACGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACAt     >  2:5163148/1‑93 (MQ=255)

                                                                                     aCGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATc    <  2:3834102/93‑1 (MQ=255)

                                                                                     aCGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATc    <  2:3930402/93‑1 (MQ=255)

                                                                                     aCGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGCCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATc    >  1:15746/1‑93 (MQ=255)

                                                                                      cGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATcc   >  1:5352580/1‑93 (MQ=255)

                                                                                      cGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATcc   >  1:3525111/1‑93 (MQ=255)

                                                                                       gggCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCg  >  1:3758859/1‑93 (MQ=255)

                                                                                       gggCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCg  <  1:3384776/93‑1 (MQ=255)

TACCCATACGCACTGCCAGCGGCTTTTCAGTGATCGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGTCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCG  >  NC_000913/3446557‑3446736

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CTTTACCCATACGCACTGCCAGCGGCTTTTCAGTGATCGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGTCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGTACC  >  NC_000913/3446554‑3446740

CTTTACCCATACGCACTGCCAGCGGCTTTTCAGTGATCGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGC                                                                                               >  SRR4302534.260404/1‑94 (MQ=60)

CTTTACCCATACGCACTGCCAGCGGCTTTTCAGTGATCGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTCGACGCTTAACTGCACGGGC                                                                                               >  SRR4302534.4677787/1‑94 (MQ=60)

  TTACCCATACGCACTGCCAGCGGCTTTTCAGTGATCGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCA                                                                                             <  SRR4302534.1951112/94‑1 (MQ=60)

  TTACCCATACGCACTGCCAGCGGCTTTTCAGTGATCGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCA                                                                                             <  SRR4302534.5852487/94‑1 (MQ=60)

  TTACCCATACGCACTGCCAGCGGCTTTTCAGTGATCGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCA                                                                                             <  SRR4302534.2551693/94‑1 (MQ=60)

   TACCCATACGCACTGCCAGCGGCTTTTCAGTGATCGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCAT                                                                                            <  SRR4302534.928081/94‑1 (MQ=60)

     CCCATACGCACTGCCAGCGGCTTTTCAGTGATCGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGTCATAG                                                                                          >  SRR4302534.281620/1‑94 (MQ=60)

     CCCATACGCACTGCCAGCGGCTTTTCAGTGATCGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAG                                                                                          >  SRR4302534.5404393/1‑94 (MQ=60)

     CCCATACGCACTGCCAGCGGCTTTTCAGTGATCGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAG                                                                                          >  SRR4302534.1973458/1‑94 (MQ=60)

     CCCATACGCACTGCCAGCGGCTTTTCAGTGATCGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAG                                                                                          <  SRR4302534.5975593/94‑1 (MQ=60)

      CCATACGCACTGCCAGCGGCTTTTCAGTGATCGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGC                                                                                         >  SRR4302534.187021/1‑94 (MQ=60)

      CCATACGCACTGCCAGCGGCTTTTCAGTGATCGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGC                                                                                         >  SRR4302534.5033724/1‑94 (MQ=60)

      CCATACGCACTGCCAGCGGCTTTTCAGTGATCGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGC                                                                                         <  SRR4302534.6044421/94‑1 (MQ=60)

      CCATACGCACTGCCAGCGGCTTTTCAGTGATCGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGC                                                                                         >  SRR4302534.228192/1‑94 (MQ=60)

      CCATACGCACTGCCAGCGGCTTTTCAGTGATCGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGC                                                                                         <  SRR4302534.2365665/94‑1 (MQ=60)

      CCATACGCACTGCCAGCGGCTTTTCAGTGATCGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGC                                                                                         >  SRR4302534.113077/1‑94 (MQ=60)

      CCATACGCACTGCCAGCGGCTTTTCAGTGATCGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGC                                                                                         >  SRR4302534.5733030/1‑94 (MQ=60)

      CCATACGCACTGCCAGCGGCTTTTCAGTGATCGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGC                                                                                         <  SRR4302534.5437432/94‑1 (MQ=60)

      CCATACGCACTGCCAGCGGCTTTTCAGTGATCGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGC                                                                                         >  SRR4302534.3471625/1‑94 (MQ=60)

      CCATACGCACTGCCAGCGGCTTTTCAGTGATCGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGC                                                                                         >  SRR4302534.6047558/1‑94 (MQ=60)

       CATACGCACTGCCAGCGGCTTTTCAGTGATCGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCA                                                                                        <  SRR4302534.4748131/94‑1 (MQ=60)

        ATACGCACTGCCAGCGGCTTTTCAGTGATCGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCAC                                                                                       <  SRR4302534.5689697/94‑1 (MQ=60)

        ATACGCACTGCCAGCGGCTTTTCAGTGATCGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCAC                                                                                       <  SRR4302534.1591615/94‑1 (MQ=60)

        ATACGCACTGCCAGCGGCTTTTCAGTGATCGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCAC                                                                                       >  SRR4302534.1504358/1‑94 (MQ=60)

          ACGCACTGCCAGCGGCTTTTCAGTGATCGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCACGA                                                                                     <  SRR4302534.4830139/94‑1 (MQ=60)

           CGCCCTGCCAGCGGCTTTTCAGTGATCGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCACGAC                                                                                    <  SRR4302534.5411321/94‑1 (MQ=60)

           CGCACTGCCAGCGGCTTTTCAGTGATCGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCACGAC                                                                                    <  SRR4302534.5319843/94‑1 (MQ=60)

            GCACTGCCAGCGGCTTTTCAGTGATCGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCACGACG                                                                                   <  SRR4302534.1097403/94‑1 (MQ=60)

            GCACTGCCAGCGGCTTTTCAGTGATCGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCACGACG                                                                                   <  SRR4302534.1654807/94‑1 (MQ=60)

            GCACTGCCAGCGGCTTTTCAGTGATCGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCACGACG                                                                                   <  SRR4302534.5190196/94‑1 (MQ=60)

            GCACTGCCAGCGGCTTTTCAGTGATCGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCACGACG                                                                                   >  SRR4302534.5915695/1‑94 (MQ=60)

             CACTGCCAGCGGCTTTTCAGTGATCGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCACGACGT                                                                                  >  SRR4302534.947110/1‑94 (MQ=60)

              ACTGCCAGCGGCTTTTCAGTGATCGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCACGACGTG                                                                                 >  SRR4302534.2639655/1‑94 (MQ=60)

               CTGCCAGCGGCTTTTCAGTGATCGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCACGACGTGC                                                                                >  SRR4302534.3153312/1‑94 (MQ=60)

                TGCCAGCGGCTTTTCAGTGATCGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCACGACGTGCT                                                                               >  SRR4302534.3633631/1‑94 (MQ=60)

                 GCCAGCGGCTTTTCAGTGATCGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCACGACGTGCTG                                                                              >  SRR4302534.716111/1‑94 (MQ=60)

                  CCAGCGGCTTTTCAGTGATCGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGC                                                                             >  SRR4302534.601877/1‑94 (MQ=60)

                   CAGCGGCTTTTCAGTGATCGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCT                                                                            >  SRR4302534.335173/1‑94 (MQ=60)

                    AGCGGCTTTTCAGTGATCGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCGATAGCACGACGTGCTGCTT                                                                           >  SRR4302534.5303612/1‑94 (MQ=60)

                    AGCGGCTTTTCAGTGATCGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTT                                                                           >  SRR4302534.4297439/1‑94 (MQ=60)

                    AGCGGCTTTTCAGTGATCGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTT                                                                           >  SRR4302534.6588031/1‑94 (MQ=60)

                    AGCGGCTTTTCAGTGATCGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTT                                                                           <  SRR4302534.3454303/94‑1 (MQ=60)

                     GCGGCTTTTCAGTGATCGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCGATAGCACGACGTGCTGCTTC                                                                          <  SRR4302534.6483224/94‑1 (MQ=60)

                     GCGGCTTTTCAGTGATCGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTC                                                                          >  SRR4302534.4579462/1‑94 (MQ=60)

                     GCGGCTTTTCAGTGATCGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTC                                                                          <  SRR4302534.1843134/94‑1 (MQ=60)

                     GCGGCTTTTCAGTGATCGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTC                                                                          <  SRR4302534.2409285/94‑1 (MQ=60)

                      CGGCTTTTCAGTGATCGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCG                                                                         <  SRR4302534.6165436/94‑1 (MQ=60)

                          TTTTCAGTGATCGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCT                                                                     <  SRR4302534.342086/94‑1 (MQ=60)

                          TTTTCAGTGATCGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCT                                                                     >  SRR4302534.5128330/1‑94 (MQ=60)

                           TTTCAGTGATCGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTG                                                                    <  SRR4302534.3543585/94‑1 (MQ=60)

                            TTCAGTGATCGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGA                                                                   <  SRR4302534.5212948/94‑1 (MQ=60)

                            TTCAGTGATCGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGA                                                                   <  SRR4302534.5949894/94‑1 (MQ=60)

                             TCAGTGATCGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGAC                                                                  >  SRR4302534.5361510/1‑94 (MQ=60)

                             TCAGTGATCGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGAC                                                                  >  SRR4302534.6244765/1‑94 (MQ=60)

                             TCAGTGATCGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGAC                                                                  <  SRR4302534.1667437/94‑1 (MQ=60)

                              CAGTGATCGTTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGACG                                                                 <  SRR4302534.3704977/94‑1 (MQ=60)

                              CAGTGATCGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGACG                                                                 >  SRR4302534.1145856/1‑94 (MQ=60)

                               AGTGATCGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGACGG                                                                >  SRR4302534.1215304/1‑94 (MQ=60)

                               AGTGATCGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGACGG                                                                <  SRR4302534.5866222/94‑1 (MQ=60)

                                GTGATCGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGG                                                               <  SRR4302534.5131131/94‑1 (MQ=60)

                                  GATCGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCA                                                             <  SRR4302534.2270012/94‑1 (MQ=60)

                                  GATCGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCA                                                             <  SRR4302534.3881729/94‑1 (MQ=60)

                                   ATCGGTTTGTCCGGGAACACACGGATCCAGATCTTACGTTGACGCTTAACTGCACGGCCCATAGCACCACGTGCTGCTTCGATCTGACGGGCAG                                                            <  SRR4302534.2049141/94‑1 (MQ=60)

                                   ATCGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAG                                                            >  SRR4302534.896540/1‑94 (MQ=60)

                                      GGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCA                                                         <  SRR4302534.4553049/94‑1 (MQ=60)

                                      GGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCA                                                         <  SRR4302534.4177048/94‑1 (MQ=60)

                                        TTTGTCCGGGAACACACGGCTCCAGATCTTACCTTGACGCTTTACTGCACGCGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGA                                                       >  SRR4302534.6349245/1‑94 (MQ=60)

                                           GTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGA                                                    >  SRR4302534.4681987/1‑94 (MQ=60)

                                           GTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGA                                                    >  SRR4302534.4527422/1‑94 (MQ=60)

                                             CCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACC                                                  <  SRR4302534.6068357/94‑1 (MQ=60)

                                               GGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCAC                                                >  SRR4302534.3964799/1‑94 (MQ=60)

                                               GGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCAC                                                >  SRR4302534.6038008/1‑94 (MQ=60)

                                                 GAACCCACGGATCCAGATCTTCCCTTGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGG                                              <  SRR4302534.3711103/94‑1 (MQ=60)

                                                 GAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGG                                              <  SRR4302534.696594/94‑1 (MQ=60)

                                                  AACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGC                                             >  SRR4302534.1543142/1‑94 (MQ=60)

                                                   ACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCC                                            >  SRR4302534.6623343/1‑94 (MQ=60)

                                                    CACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCA                                           >  SRR4302534.5833358/1‑94 (MQ=60)

                                                    CACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCA                                           >  SRR4302534.372170/1‑94 (MQ=60)

                                                     ACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGTCAA                                          >  SRR4302534.4898594/1‑94 (MQ=60)

                                                     ACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAA                                          <  SRR4302534.435664/94‑1 (MQ=60)

                                                     ACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAA                                          >  SRR4302534.3193523/1‑94 (MQ=60)

                                                     ACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAA                                          >  SRR4302534.4627970/1‑94 (MQ=60)

                                                     ACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAA                                          >  SRR4302534.838512/1‑94 (MQ=60)

                                                          GATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATGTGACGGGCAGTCAGACGACCACGGCCAACAGCT                                     <  SRR4302534.5872348/94‑1 (MQ=60)

                                                          GATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCT                                     <  SRR4302534.2081264/94‑1 (MQ=60)

                                                           ATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGCCGACCACGGCCAACAGCTT                                    >  SRR4302534.4711719/1‑94 (MQ=60)

                                                           ATCCAGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTT                                    <  SRR4302534.5889835/94‑1 (MQ=60)

                                                             CCAGATCTTCCCTTGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTC                                  <  SRR4302534.310605/94‑1 (MQ=60)

                                                               AGATCTTACCTTGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAG                                >  SRR4302534.2801356/1‑94 (MQ=60)

                                                                GATCTTACCTTGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATATGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGA                               >  SRR4302534.2999115/1‑94 (MQ=60)

                                                                  TCTTACCTTGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACC                             <  SRR4302534.5464699/94‑1 (MQ=60)

                                                                   CTTACCTTGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCG                            >  SRR4302534.1347133/1‑94 (MQ=60)

                                                                    TTACCTTGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGA                           >  SRR4302534.3156063/1‑94 (MQ=60)

                                                                     TACCTTGACGCTTAACTGCACGGGCCATAGCACGTCGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAA                          >  SRR4302534.2013885/1‑94 (MQ=60)

                                                                     TACCTTGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAA                          >  SRR4302534.5860424/1‑94 (MQ=60)

                                                                     TACCTTGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAA                          >  SRR4302534.5811156/1‑94 (MQ=60)

                                                                     TACCTTGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAA                          <  SRR4302534.1224356/94‑1 (MQ=60)

                                                                      AGCTTCACGCTCAACTGCCCGGGCCATAGCACGACGTGCTACTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAG                         <  SRR4302534.5025062/94‑1 (MQ=60)

                                                                      ACCTTGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAG                         <  SRR4302534.2431661/94‑1 (MQ=60)

                                                                      ACCTTGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAG                         >  SRR4302534.1426036/1‑94 (MQ=60)

                                                                       CCTTGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGC                        >  SRR4302534.4322835/1‑94 (MQ=60)

                                                                       CCTTGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGC                        <  SRR4302534.3406907/94‑1 (MQ=60)

                                                                         TTGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTG                      >  SRR4302534.1792783/1‑94 (MQ=60)

                                                                         TTGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTG                      >  SRR4302534.3567767/1‑94 (MQ=60)

                                                                          TGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGC                     >  SRR4302534.4582951/1‑94 (MQ=60)

                                                                          TGACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGC                     >  SRR4302534.4264820/1‑94 (MQ=60)

                                                                           GACGCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCC                    <  SRR4302534.3883463/94‑1 (MQ=60)

                                                                              GCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAA                 >  SRR4302534.6198039/1‑94 (MQ=60)

                                                                              GCTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAA                 <  SRR4302534.2147417/94‑1 (MQ=60)

                                                                              GATTAACTGCACGGGCCATAGCACGACGTGCTGATTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCACAGCTGCCGAA                 >  SRR4302534.2362389/1‑94 (MQ=60)

                                                                               CTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAG                >  SRR4302534.5244268/1‑94 (MQ=60)

                                                                               CTTAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAG                <  SRR4302534.2981618/94‑1 (MQ=60)

                                                                               CTGAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAG                <  SRR4302534.2954331/94‑1 (MQ=60)

                                                                                 TAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCT              >  SRR4302534.2585491/1‑94 (MQ=60)

                                                                                 TAACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCT              >  SRR4302534.1577417/1‑94 (MQ=60)

                                                                                 TAACGGCAGGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCT              <  SRR4302534.1654548/94‑1 (MQ=60)

                                                                                  AACTGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTA             >  SRR4302534.3171618/1‑94 (MQ=60)

                                                                                     TGCCCGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACA          <  SRR4302534.24897/94‑1 (MQ=60)

                                                                                     TGCACGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACA          >  SRR4302534.6580578/1‑94 (MQ=60)

                                                                                      GCACGGGCCATAGCACGACTGGCTGCTTCGATCTGACAGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAATCTGCCGCAGCTAACAT         >  SRR4302534.631406/1‑94 (MQ=60)

                                                                                      GCACGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACAT         <  SRR4302534.1541368/94‑1 (MQ=60)

                                                                                      GCACGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACAT         <  SRR4302534.2467067/94‑1 (MQ=60)

                                                                                       CACGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATC        <  SRR4302534.4681740/94‑1 (MQ=60)

                                                                                       CACGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGCCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATC        >  SRR4302534.16169/1‑94 (MQ=60)

                                                                                        ACGGGCCATAGCACGAGGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCC       <  SRR4302534.3766850/94‑1 (MQ=60)

                                                                                        ACGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCC       >  SRR4302534.5496887/1‑94 (MQ=60)

                                                                                        ACGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCC       >  SRR4302534.3619924/1‑94 (MQ=60)

                                                                                        ACGCGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCC       <  SRR4302534.3363916/94‑1 (MQ=60)

                                                                                         CGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCG      <  SRR4302534.996078/94‑1 (MQ=60)

                                                                                         CGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCG      >  SRR4302534.3859790/1‑94 (MQ=60)

                                                                                         CGGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCG      >  SRR4302534.1749233/1‑94 (MQ=60)

                                                                                          GGGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGC     <  SRR4302534.3475534/94‑1 (MQ=60)

                                                                                           GGCCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCA    >  SRR4302534.4570696/1‑94 (MQ=60)

                                                                                             CCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACC  >  SRR4302534.1496410/1‑94 (MQ=60)

                                                                                             CCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACC  >  SRR4302534.5229654/1‑94 (MQ=60)

CTTTACCCATACGCACTGCCAGCGGCTTTTCAGTGATCGGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGTCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGTACC  >  NC_000913/3446554‑3446740

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`