Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	884	257.2	12958910	96.9%	12557183	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,469,235	A→G	R639R (CGT→CGC)	fusA ←	GTP‑binding protein chain elongation factor EF‑G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,469,235	0	A	G	99.6%	815.8 / NA	233	R639R (CGT→CGC)	fusA	GTP‑binding protein chain elongation factor EF‑G
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (121/111); minor base T (0/1); total (121/112)
Fisher's exact test for biased strand distribution p-value = 4.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 249 total aligned reads displayed.

TGAGTTGCGTATCCGAACATTTCAGACAGCGGTACTTCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCACGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTGG  >  NC_000913/3469146‑3469324

 gAGTTGCGTATCCGAACATTTCAGACAGCGGTACTTCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCgcgac                                                                                       <  1:5886162/93‑1 (MQ=255)

  aGTTGCGTATCCGAACATTTCAGACAGCGGTACTTCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCgcgacg                                                                                      >  2:2062543/1‑93 (MQ=255)

   gTTGCGTATCCTAACATTTCAGACAGCGGTACTTCATCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCgcgacga                                                                                     <  1:5337919/93‑1 (MQ=255)

    ttGCGTATCCGAACATTTCAGACAGCGGTACTTCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCgcgacgac                                                                                    >  1:5705481/1‑93 (MQ=255)

    ttGCGTATCCGAACATTTCAGACAGCGGTACTTCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCgcgacgac                                                                                    <  2:381038/93‑1 (MQ=255)

       cGTATCCGAACATTTCAGACAGCGGTACTTCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCTCGACGACGGc                                                                                 <  2:6140075/93‑1 (MQ=255)

       cGTATCCGAACATTTCAGACAGCGGTACTTCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGc                                                                                 <  2:94487/93‑1 (MQ=255)

          aTCCGAACATTTCAGACAGCGGTACTTCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCa                                                                              <  2:1914380/93‑1 (MQ=255)

           tCCGAACATTTCAGACAGCGGTACTTCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCaa                                                                             >  1:3669069/1‑93 (MQ=255)

             cGAACATTTCAGACAGCGGTACTTCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGt                                                                           >  2:6111378/1‑93 (MQ=255)

             cGAACATTTCAGACAGCGGTACTTCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGt                                                                           >  1:4675146/1‑93 (MQ=255)

             cGAACATTTCAGACAGCGGTACTTCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGt                                                                           >  2:1779961/1‑93 (MQ=255)

              gAACATTTCAGACAGCGGTACTTCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTc                                                                          >  1:4832293/1‑93 (MQ=255)

               aaCATTTCAGACAGCGGTACTTCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCa                                                                         <  1:5249028/93‑1 (MQ=255)

                    ttCAGACAGCGGTACTTCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGAt                                                                    <  2:3625724/93‑1 (MQ=255)

                       aGACAGCGGTACTTCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAAc                                                                 <  1:6392415/93‑1 (MQ=255)

                        gACAGCGGTACTTCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACg                                                                >  1:1449569/1‑93 (MQ=255)

                        gACAGCGGTACTTCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACg                                                                >  1:5233589/1‑93 (MQ=255)

                          cAGCGGTACTTCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTc                                                              >  1:5463429/1‑93 (MQ=255)

                           aGCGGTACTTCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCa                                                             >  1:5310449/1‑93 (MQ=255)

                            gCGGTACTTCAGCGTTGATCTTAGCGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCAc                                                            <  1:3249592/93‑1 (MQ=255)

                            gCGGTACTTCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCAc                                                            <  2:5034736/93‑1 (MQ=255)

                            gCGGTACTTCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCAc                                                            <  2:1401404/93‑1 (MQ=255)

                            gCGGTACTTCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCAc                                                            >  1:5116395/1‑93 (MQ=255)

                            gCGGTACTTCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCAc                                                            <  2:1794338/93‑1 (MQ=255)

                            gCGGTACTTCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCAc                                                            <  2:3780017/93‑1 (MQ=255)

                             cGGTACTTCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCAcc                                                           <  1:3577849/93‑1 (MQ=255)

                               gTACTTCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCgg                                                         >  2:2244779/1‑93 (MQ=255)

                               gTACTTCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCgg                                                         >  1:2633452/1‑93 (MQ=255)

                                 aCTTCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGgtg                                                       >  1:2121965/1‑93 (MQ=255)

                                  cTTCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGgtgt                                                      >  1:4309998/1‑93 (MQ=255)

                                  cTTCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGgtgt                                                      <  1:3177112/93‑1 (MQ=255)

                                   ttCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGtt                                                     <  1:1392185/93‑1 (MQ=255)

                                   ttCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGtt                                                     <  2:5949461/93‑1 (MQ=255)

                                    tCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTtc                                                    >  2:2987606/1‑93 (MQ=255)

                                     cAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTtct                                                   >  1:5074745/1‑93 (MQ=255)

                                     cAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTtct                                                   >  2:5559360/1‑93 (MQ=255)

                                     cAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTtct                                                   <  2:5838869/93‑1 (MQ=255)

                                      aGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTtctc                                                  <  2:1662534/93‑1 (MQ=255)

                                       gCGTGGATCTTAACGCCAGTAACTTCAGCTTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTctct                                                 <  2:1513717/93‑1 (MQ=255)

                                       gCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTctct                                                 >  1:1803191/1‑93 (MQ=255)

                                       gCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTctct                                                 >  2:3101/1‑93 (MQ=255)

                                        cGTGGATCTTATCGCCAGTAACTTCAGATTCCTGACCTTTAAGCATACGTCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTGtt                                                <  1:3874768/93‑1 (MQ=255)

                                         gTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTc                                               <  1:1125913/93‑1 (MQ=255)

                                         gTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTc                                               >  1:3004185/1‑93 (MQ=255)

                                          tGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTcc                                              >  1:5553060/1‑93 (MQ=255)

                                           ggATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCg                                             <  2:2108646/93‑1 (MQ=255)

                                           ggATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCg                                             >  2:742446/1‑93 (MQ=255)

                                            gATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCgg                                            >  2:6342508/1‑93 (MQ=255)

                                             aTCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGa                                           <  2:1610549/93‑1 (MQ=255)

                                             aTCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGa                                           >  2:268658/1‑93 (MQ=255)

                                               cTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGt                                         <  2:2132719/93‑1 (MQ=255)

                                               cTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGt                                         <  1:1742802/93‑1 (MQ=255)

                                                ttAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGtt                                        <  2:1223745/93‑1 (MQ=255)

                                                ttAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGtt                                        <  1:2748261/93‑1 (MQ=255)

                                                ttAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGtt                                        >  1:5999003/1‑93 (MQ=255)

                                                ttAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGtt                                        <  2:709420/93‑1 (MQ=255)

                                                 tAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGttt                                       <  2:4229217/93‑1 (MQ=255)

                                                  aaCGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTc                                      <  1:5655812/93‑1 (MQ=255)

                                                  aaCGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTc                                      >  1:2548128/1‑93 (MQ=255)

                                                   aCGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCt                                     >  1:27919/1‑93 (MQ=255)

                                                    cGCCAGTAACTTCAGATTCCTGACCTTTGAGCATGCCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTa                                    <  2:6411066/93‑1 (MQ=255)

                                                     gCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTAc                                   <  1:1281310/93‑1 (MQ=255)

                                                       cAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACtt                                 >  1:1007270/1‑93 (MQ=255)

                                                       cAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACtt                                 >  1:4199973/1‑93 (MQ=255)

                                                       cAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACtt                                 >  2:1018848/1‑93 (MQ=255)

                                                        aGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTc                                >  2:5964511/1‑93 (MQ=255)

                                                        aGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTc                                >  1:95289/1‑93 (MQ=255)

                                                         gTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCa                               >  1:4011678/1‑93 (MQ=255)

                                                          tAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCaa                              >  1:2408835/1‑93 (MQ=255)

                                                            aCTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAAcc                            >  2:2470685/1‑93 (MQ=255)

                                                              ttCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCtt                          >  2:2953839/1‑93 (MQ=255)

                                                               tCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTc                         <  2:3536687/93‑1 (MQ=255)

                                                                cAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCa                        <  1:119200/93‑1 (MQ=255)

                                                                cAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCa                        >  2:654068/1‑93 (MQ=255)

                                                                cAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCa                        >  2:4988387/1‑93 (MQ=255)

                                                                 aGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCac                       >  1:4920606/1‑92 (MQ=255)

                                                                 aGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCAt                       <  2:2798622/93‑1 (MQ=255)

                                                                 aGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCAt                       <  2:4650653/93‑1 (MQ=255)

                                                                  gATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATg                      >  1:2716803/1‑93 (MQ=255)

                                                                  gATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATg                      >  1:67517/1‑93 (MQ=255)

                                                                    ttCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGAt                    <  1:473265/93‑1 (MQ=255)

                                                                     tCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATc                   <  2:5508576/93‑1 (MQ=255)

                                                                      ccTGTCCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCg                  <  2:875464/93‑1 (MQ=255)

                                                                      ccTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCg                  >  2:4197819/1‑93 (MQ=255)

                                                                       cTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCgg                 <  2:5338374/93‑1 (MQ=255)

                                                                        tGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGc                <  1:2895594/93‑1 (MQ=255)

                                                                        tGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGc                >  2:2646822/1‑93 (MQ=255)

                                                                        tGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGc                <  1:305956/93‑1 (MQ=255)

                                                                        tGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGc                <  1:3486102/93‑1 (MQ=255)

                                                                         gACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCt               <  1:2269905/93‑1 (MQ=255)

                                                                           ccTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCTCa             >  1:6144277/1‑93 (MQ=255)

                                                                              ttGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCTCAAGc          <  2:2415287/93‑1 (MQ=255)

                                                                               tGAGCATACCCCGACGCCGGCTCAAGTCACCGATACCGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCa         <  2:2911667/93‑1 (MQ=255)

                                                                                gAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAg        >  2:4780976/1‑93 (MQ=255)

                                                                                gAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAg        <  2:1824813/93‑1 (MQ=255)

                                                                                  gCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGaa      >  1:6244546/1‑93 (MQ=255)

                                                                                     tACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTg   <  2:3265302/93‑1 (MQ=255)

                                                                                      aCCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTgg  >  2:6239095/1‑93 (MQ=255)

                                                                                      aCCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTgg  >  1:339196/1‑93 (MQ=255)

TGAGTTGCGTATCCGAACATTTCAGACAGCGGTACTTCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCACGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTGG  >  NC_000913/3469146‑3469324

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CAGCTGAGTTGCGTATCCGAACATTTCAGACAGCGGTACTTCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCACGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTTT  >  NC_000913/3469142‑3469327

CAGCTGAGTTGCGTATCCGAACATTTCAGACAGCGGTACTTCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCG                                                                                              <  SRR4302534.4921801/94‑1 (MQ=60)

CAGCTGAGTTGCGTATCCGAACATTTCAGACAGCGGTACTTCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCG                                                                                              <  SRR4302534.1621181/94‑1 (MQ=60)

 AGCTGAGTTGCGTATCCGAACATTTCAGACAGCGGTACTTCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGC                                                                                             >  SRR4302534.5231876/1‑94 (MQ=60)

 AGCTGAGTTGCGTATCCGAACATTTCAGACAGCGGTACTTCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGC                                                                                             >  SRR4302534.1571455/1‑94 (MQ=60)

 AGCTGAGTTGCGTATCCGAACATTACAGACAGCGGTACTTCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGC                                                                                             >  SRR4302534.5325114/1‑94 (MQ=60)

  GCTGAGTTGCGTATCCGAACATTTCAGACAGCGGTACTTCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCG                                                                                            <  SRR4302534.141322/94‑1 (MQ=60)

   CTGAGTTGCGTATCCGAACATTTCAGACAGCGGTACTTCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGA                                                                                           >  SRR4302534.3617127/1‑94 (MQ=60)

     GAGTTGCGTATCCGAACATTTCAGACAGCGGTACTTCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACG                                                                                         <  SRR4302534.6045985/94‑1 (MQ=60)

      AGTTGCGTATCCGAACATTTCAGACAGCGGTACTTCAGCGTGAATCTTAACGCCAGTAACTTCAGATTCTTGACCTTTGAGCATCCCACGACCA                                                                                        <  SRR4302534.254551/94‑1 (MQ=60)

       GTTGCGTATCCTAACATTTCAGACAGCGGTACTTCATCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGAC                                                                                       <  SRR4302534.5481836/94‑1 (MQ=60)

       GTTGCGTATCCGAACATTTCAGACAGCGGTACTTCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGAC                                                                                       >  SRR4302534.5860040/1‑94 (MQ=60)

        TTGCGTATCCGAACATTTCAGACAGCGGTACTTCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACG                                                                                      <  SRR4302534.127907/94‑1 (MQ=60)

            GTATCCGAACATTTCAGACAGCGGTACTTCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTC                                                                                  <  SRR4302534.5498286/94‑1 (MQ=60)

            GTATCCGAACATTTCAGACAGCGGTACTTCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTC                                                                                  <  SRR4302534.3444256/94‑1 (MQ=60)

             TATCCGAACATTTCAGACAGCGGTACTTCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCA                                                                                 <  SRR4302534.4206308/94‑1 (MQ=60)

             TATCCGAACATTTCAGACAGCGGTACTTCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCA                                                                                 <  SRR4302534.1818537/94‑1 (MQ=60)

             TATCCGAACATTTCAGACAGCGGTACTTCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCA                                                                                 >  SRR4302534.5754051/1‑94 (MQ=60)

              ATCCGAACATTTCAGACAGCGGTACTTCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAA                                                                                >  SRR4302534.3767702/1‑94 (MQ=60)

              ATCCGAACATTTCAGACAGCGGTACTTCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAA                                                                                >  SRR4302534.6556981/1‑94 (MQ=60)

                CCGAACATTTCAGACAGCGGTACTTCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGT                                                                              >  SRR4302534.4802051/1‑94 (MQ=60)

                CCGAACATTTCAGACAGCGGTACTTCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGT                                                                              <  SRR4302534.2461257/94‑1 (MQ=60)

                CCGAACATTTCAGACAGCGGTACTTCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGT                                                                              >  SRR4302534.1878721/1‑94 (MQ=60)

                CCGAACATTTCAGACAGCGGTACTTCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGT                                                                              >  SRR4302534.606974/1‑94 (MQ=60)

                 CGAACATTTCAGACAGCGGTACTTCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTC                                                                             >  SRR4302534.4963393/1‑94 (MQ=60)

                  GAACATTTCAGACAGCGGTACTTCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCA                                                                            >  SRR4302534.2648270/1‑94 (MQ=60)

                  GAACATTTCAGACAGCGGTACTTCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCA                                                                            <  SRR4302534.6222583/94‑1 (MQ=60)

                   AACATTTCAGACAGCGGTACTTCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCAC                                                                           <  SRR4302534.5390828/94‑1 (MQ=60)

                     CATTTCAGACAGCGGTACTTCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGACACCG                                                                         >  SRR4302534.122552/1‑94 (MQ=60)

                        TTCAGACAGCGGTACTTCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATA                                                                      <  SRR4302534.2928459/94‑1 (MQ=60)

                        TTCAGACAGCGGTACTTCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATA                                                                      <  SRR4302534.1218882/94‑1 (MQ=60)

                         TCAGACAGCGGTACTTCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAA                                                                     <  SRR4302534.1138756/94‑1 (MQ=60)

                          CAGACAGCGGTACTTCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAAC                                                                    >  SRR4302534.5137061/1‑94 (MQ=60)

                          CAGACAGCGGTACTTCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAAC                                                                    >  SRR4302534.691029/1‑94 (MQ=60)

                           AGACAGCGGTACTTCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACG                                                                   >  SRR4302534.5374885/1‑94 (MQ=60)

                           AGACAGCGGTACTTCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACG                                                                   >  SRR4302534.1487719/1‑94 (MQ=60)

                           AGACAGCGGTACTTCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACG                                                                   <  SRR4302534.6565066/94‑1 (MQ=60)

                            GACAGCGGTACTTCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGT                                                                  >  SRR4302534.5412942/1‑94 (MQ=60)

                             ACAGCGGTACTTCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTC                                                                 >  SRR4302534.5491134/1‑94 (MQ=60)

                             ACAGCGGTACTTCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTC                                                                 >  SRR4302534.5610688/1‑94 (MQ=60)

                             ACAGCGGTACTTCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTC                                                                 <  SRR4302534.4764154/94‑1 (MQ=60)

                              CAGCGGTACTTCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCA                                                                <  SRR4302534.2262230/94‑1 (MQ=60)

                              CAGCGGTACTTCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCA                                                                >  SRR4302534.5453733/1‑94 (MQ=60)

                              CAGCGGTACTTCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCA                                                                >  SRR4302534.3199774/1‑94 (MQ=60)

                               AGCGGTACTTCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCAC                                                               >  SRR4302534.5254478/1‑94 (MQ=60)

                                GCGGTACTTCAGCGTTGATCTTAGCGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACC                                                              <  SRR4302534.3336128/94‑1 (MQ=60)

                                GCGGTACTTCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACC                                                              <  SRR4302534.5982305/94‑1 (MQ=60)

                                GCGGTACTTCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACC                                                              <  SRR4302534.3839655/94‑1 (MQ=60)

                                 CGGTACTTCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCG                                                             >  SRR4302534.3044206/1‑94 (MQ=60)

                                 CGGTACTTCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCG                                                             >  SRR4302534.2180026/1‑94 (MQ=60)

                                 CGGTACTTCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCG                                                             <  SRR4302534.3674085/94‑1 (MQ=60)

                                 CGGTACTTCAGCGTGGATCTCAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCG                                                             >  SRR4302534.6498637/1‑94 (MQ=60)

                                  GGTACTTCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGG                                                            >  SRR4302534.2703137/1‑94 (MQ=60)

                                    TACTTCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTG                                                          >  SRR4302534.2177838/1‑94 (MQ=60)

                                     ACTTCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGT                                                         >  SRR4302534.4957558/1‑94 (MQ=60)

                                     ACTTCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGT                                                         >  SRR4302534.4426770/1‑94 (MQ=60)

                                     ACTTCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGT                                                         >  SRR4302534.2859393/1‑94 (MQ=60)

                                      CTTCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTT                                                        <  SRR4302534.3260999/94‑1 (MQ=60)

                                      CTTCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTT                                                        >  SRR4302534.5378077/1‑94 (MQ=60)

                                      CTTCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTT                                                        >  SRR4302534.2724818/1‑94 (MQ=60)

                                       TTCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTC                                                       <  SRR4302534.1428828/94‑1 (MQ=60)

                                       TTCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTC                                                       >  SRR4302534.4700317/1‑94 (MQ=60)

                                        TCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCT                                                      >  SRR4302534.5211735/1‑94 (MQ=60)

                                         CAGCGTGGATCTTAACGCCAGTAACTTTAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTC                                                     <  SRR4302534.98750/94‑1 (MQ=60)

                                         CAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTC                                                     <  SRR4302534.4950756/94‑1 (MQ=60)

                                          AGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCT                                                    >  SRR4302534.5082913/1‑94 (MQ=60)

                                          AGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCT                                                    >  SRR4302534.1850024/1‑94 (MQ=60)

                                          AGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCT                                                    <  SRR4302534.1347814/94‑1 (MQ=60)

                                            CGTGGATCTTATCGCCAGTAACTTCAGATTCCTGACCTTTAAGCATACGTCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTGTTC                                                  <  SRR4302534.3978798/94‑1 (MQ=60)

                                            CGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTC                                                  >  SRR4302534.3082775/1‑94 (MQ=60)

                                            CGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTC                                                  >  SRR4302534.373522/1‑94 (MQ=60)

                                             GTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCC                                                 <  SRR4302534.5357572/94‑1 (MQ=60)

                                             GTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCC                                                 <  SRR4302534.1155336/94‑1 (MQ=60)

                                             GTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCC                                                 >  SRR4302534.180213/1‑94 (MQ=60)

                                             GTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCC                                                 >  SRR4302534.6246822/1‑94 (MQ=60)

                                             GTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCC                                                 >  SRR4302534.5702966/1‑94 (MQ=60)

                                             GTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCC                                                 <  SRR4302534.5774509/94‑1 (MQ=60)

                                              TGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCG                                                <  SRR4302534.5039909/94‑1 (MQ=60)

                                                GATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGA                                              <  SRR4302534.1456727/94‑1 (MQ=60)

                                                GATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGA                                              <  SRR4302534.2792313/94‑1 (MQ=60)

                                                  TCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGT                                            <  SRR4302534.5455073/94‑1 (MQ=60)

                                                   CTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTT                                           >  SRR4302534.6161980/1‑94 (MQ=60)

                                                   CTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTT                                           >  SRR4302534.432146/1‑94 (MQ=60)

                                                   CTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTT                                           <  SRR4302534.1788038/94‑1 (MQ=60)

                                                    TTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTT                                          <  SRR4302534.2820664/94‑1 (MQ=60)

                                                     TAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTC                                         >  SRR4302534.2615693/1‑94 (MQ=60)

                                                      AACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCT                                        <  SRR4302534.2851831/94‑1 (MQ=60)

                                                      AACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCT                                        <  SRR4302534.5808920/94‑1 (MQ=60)

                                                      AACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCT                                        >  SRR4302534.28678/1‑94 (MQ=60)

                                                       ACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTA                                       <  SRR4302534.5813054/94‑1 (MQ=60)

                                                         GCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACT                                     <  SRR4302534.1315035/94‑1 (MQ=60)

                                                          CCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTT                                    >  SRR4302534.4313393/1‑94 (MQ=60)

                                                          CCAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTT                                    >  SRR4302534.1033123/1‑94 (MQ=60)

                                                           CAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTC                                   <  SRR4302534.945482/94‑1 (MQ=60)

                                                           CAGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTC                                   >  SRR4302534.97838/1‑94 (MQ=60)

                                                            AGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCA                                  >  SRR4302534.4119118/1‑94 (MQ=60)

                                                            AGTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCA                                  >  SRR4302534.3238711/1‑94 (MQ=60)

                                                             GTGACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAA                                 >  SRR4302534.5257424/1‑94 (MQ=60)

                                                             GTAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAA                                 >  SRR4302534.2472868/1‑94 (MQ=60)

                                                              TAACTTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAAC                                >  SRR4302534.3355157/1‑94 (MQ=60)

                                                                  TTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTC                            >  SRR4302534.5397248/1‑94 (MQ=60)

                                                                  TTCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTC                            >  SRR4302534.2754807/1‑94 (MQ=60)

                                                                   TCAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCA                           <  SRR4302534.4135833/94‑1 (MQ=60)

                                                                    CAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCAT                          <  SRR4302534.122368/94‑1 (MQ=60)

                                                                    CAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCAT                          >  SRR4302534.3084559/1‑94 (MQ=60)

                                                                    CAGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCAC                          >  SRR4302534.5053971/1‑94 (MQ=60)

                                                                     AGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATG                         >  SRR4302534.69356/1‑94 (MQ=60)

                                                                     AGATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATG                         >  SRR4302534.2788505/1‑94 (MQ=60)

                                                                       ATTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGAT                       <  SRR4302534.25051/94‑1 (MQ=60)

                                                                        TTCCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATC                      <  SRR4302534.485173/94‑1 (MQ=60)

                                                                        CTCCTGACGTTTGAGCACACGGCGACGACGGCTCAAGCCACCGATAACGTCCCCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATC                      <  SRR4302534.3641697/94‑1 (MQ=60)

                                                                         TCCTGACCTTTGAGCATACCGGGACGACGGCTCATGTCACCGATACCGTCACCGGTGTTCTCTTCCGGAGTTTCTCCTTCACCCTTCATGATCG                     <  SRR4302534.3329998/94‑1 (MQ=60)

                                                                          CCTGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGG                    <  SRR4302534.523588/94‑1 (MQ=60)

                                                                            TGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCT                  <  SRR4302534.2971598/94‑1 (MQ=60)

                                                                            TGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCT                  <  SRR4302534.5653131/94‑1 (MQ=60)

                                                                            TGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCT                  <  SRR4302534.3579799/94‑1 (MQ=60)

                                                                            TGACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCT                  <  SRR4302534.313845/94‑1 (MQ=60)

                                                                            TAACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCT                  >  SRR4302534.1477032/1‑94 (MQ=60)

                                                                             GACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCTC                 <  SRR4302534.2330092/94‑1 (MQ=60)

                                                                             GACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCTC                 >  SRR4302534.4103922/1‑94 (MQ=60)

                                                                             GACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCTC                 <  SRR4302534.4839073/94‑1 (MQ=60)

                                                                              ACCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCTCA                >  SRR4302534.6311375/1‑94 (MQ=60)

                                                                               CCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCTCAA               >  SRR4302534.3039614/1‑94 (MQ=60)

                                                                               CCTTTGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCTCAA               >  SRR4302534.3558245/1‑94 (MQ=60)

                                                                                   TGAGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAG           <  SRR4302534.5949583/94‑1 (MQ=60)

                                                                                    GAGCATACCGCGACGCCGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGA          >  SRR4302534.1787369/1‑94 (MQ=60)

                                                                                     AGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAA         >  SRR4302534.6349981/1‑94 (MQ=60)

                                                                                     AGCATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAA         >  SRR4302534.6413955/1‑94 (MQ=60)

                                                                                       CATACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAACT       <  SRR4302534.3912051/94‑1 (MQ=60)

                                                                                         TACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTGG     >  SRR4302534.347874/1‑94 (MQ=60)

                                                                                         TACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTGG     >  SRR4302534.726198/1‑94 (MQ=60)

                                                                                          ACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGGACTGGT    >  SRR4302534.4120377/1‑94 (MQ=60)

                                                                                          ACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTGGT    >  SRR4302534.6418836/1‑94 (MQ=60)

                                                                                          ACCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTGGT    >  SRR4302534.2070430/1‑94 (MQ=60)

                                                                                           CCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTT   <  SRR4302534.1781229/94‑1 (MQ=60)

                                                                                           CCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTT   <  SRR4302534.3656687/94‑1 (MQ=60)

                                                                                           CCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTT   <  SRR4302534.3790117/94‑1 (MQ=60)

                                                                                           CCGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTT   >  SRR4302534.3705142/1‑94 (MQ=60)

                                                                                            CGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTTT  <  SRR4302534.3422925/94‑1 (MQ=60)

                                                                                            CGCGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTTT  <  SRR4302534.4877455/94‑1 (MQ=60)

CAGCTGAGTTGCGTATCCGAACATTTCAGACAGCGGTACTTCAGCGTGGATCTTAACGCCAGTAACTTCAGATTCCTGACCTTTGAGCATACCACGACGACGGCTCAAGTCACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTTT  >  NC_000913/3469142‑3469327

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`