Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	884	257.2	12958910	96.9%	12557183	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,500,309	Δ1 bp	coding (333/363 nt)	b3372 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,500,308	0	C	.	99.6%	1073.9 / ‑3.4	230	coding (332/363 nt)	b3372	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (1/0); new base . (133/96); total (134/96)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01

Only 100 of 251 total aligned reads displayed.

CGATGAACATATGCGTCGCGAAAGCCTCGACATGATCAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCGCCGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAGAACCTAAGCGAACTGTGTGAGTACGCGGAAAA  >  NC_000913/3500216‑3500396

ggATGAACATATGCGTCGCGAAAGCCTCGACATGATCAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTccgc                                                                                          <  2:6105745/92‑1 (MQ=255)

 gATGAACATATGCGTCGCGAAAGCCTCGACATGATCAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTccgcg                                                                                         >  1:1297023/1‑92 (MQ=255)

 gATGAACATATGCGTCGCGAAAGCCTCGACATGATCAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTccgcg                                                                                         <  1:5086596/93‑2 (MQ=255)

 gATGAACATATGCGTCGCGAAAGCCTCGACATGATCAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTccgcg                                                                                         >  2:5316666/1‑92 (MQ=255)

  aTGCACATATGCGTCGCGAAAGCCTCGACATGATCAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTccgcgg                                                                                        >  1:5330552/1‑91 (MQ=255)

  aTGAACATATGCGTCGCGAAAGCCTCGACATGATCAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTccgcgg                                                                                        >  1:6256135/1‑91 (MQ=255)

    gAACATATGCGTCGCGAAAGCCTCGACATGATCAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTccgcggcc                                                                                      >  2:4071722/1‑89 (MQ=255)

     aaCATATGCGTCGCGAAAGCCTCGACATGATCAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGccc                                                                                    >  1:1373491/1‑93 (MQ=255)

     aaCATATGCGTCGCGAAAGCCTCGACATGATCAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGccc                                                                                    <  2:3368959/93‑1 (MQ=255)

      aCATATGCGTCGCGAAAGCCTCGACATGATCAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCGCGGGcca                                                                                    >  1:1647973/1‑92 (MQ=255)

        atatGCGTCGCGAAAGCCTCGACATGATCAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCAcg                                                                                 >  1:6118986/1‑93 (MQ=255)

        atatGCGTCGCGAAAGCCTCGACATGATCAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCAcg                                                                                 >  2:1422680/1‑93 (MQ=255)

         tatGCGTCGCGAAAGCCTCGACATGATCAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCAcgc                                                                                <  2:2117427/93‑1 (MQ=255)

         tatGCGTCGCGAAAGCCTCGACATGATCAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCAcgc                                                                                >  1:388514/1‑93 (MQ=255)

           tGCGTCGCGAAAGCCTCGACATGATCAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCgg                                                                              >  2:680228/1‑93 (MQ=255)

            gCGTCGCGAAAGCCTCGACATGATCAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCggg                                                                             >  2:3607976/1‑93 (MQ=255)

              gTCGCGAAAGCCTCGACATGATCAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCt                                                                           <  1:3017544/93‑1 (MQ=255)

               tCGCGAAAGCCTCGACATGATCAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTa                                                                          <  2:2327382/93‑1 (MQ=255)

                cgcgAAAGCCTCGACATGATCAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTAt                                                                         <  2:1638066/93‑1 (MQ=255)

                 gcgAAAGCCTCGACATGATCAAGCTGGCGATGTATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTAtc                                                                        <  1:2083715/93‑1 (MQ=255)

                 gcgAAAGCCTCGACATGATCAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTAtc                                                                        <  1:908709/93‑1 (MQ=255)

                  cgAAAGCCTCGACATGATCAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTAtct                                                                       <  1:1392316/93‑1 (MQ=255)

                   gAAAGCCTCGACATGATCAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTAtctc                                                                      <  2:3561168/93‑1 (MQ=255)

                   gAAAGCCTCGACATGATCAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTAtctc                                                                      <  2:4907874/93‑1 (MQ=255)

                    aaaGCCTCGACATGATCAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCa                                                                     >  1:3459636/1‑93 (MQ=255)

                     aaGCCTCGACATGATCAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCAc                                                                    <  2:220866/93‑1 (MQ=255)

                      aGCCTCGACATGATCAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACg                                                                   >  2:3108936/1‑93 (MQ=255)

                       gCCTCGACATGATCAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGc                                                                  <  1:2771077/93‑1 (MQ=255)

                         cTCGACATGATCAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCa                                                                >  1:6181582/1‑93 (MQ=255)

                          tCGACATGATCAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCAc                                                               >  2:2644863/1‑93 (MQ=255)

                           cGACATGATCAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTTACGCCAcc                                                              <  1:6298506/93‑1 (MQ=255)

                           cGACATGATCAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCAcc                                                              <  1:4990091/93‑1 (MQ=255)

                            gACATGATCAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCt                                                             >  2:1731623/1‑93 (MQ=255)

                            gACATGATCAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCt                                                             <  1:983920/93‑1 (MQ=255)

                            gACATGATCAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCt                                                             >  1:2331934/1‑93 (MQ=255)

                              cATGATCAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTaa                                                           <  2:5585459/93‑1 (MQ=255)

                                 gATCAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGt                                                        <  1:4631312/93‑1 (MQ=255)

                                 gATCAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGt                                                        <  2:5468946/93‑1 (MQ=255)

                                  aTCAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGtt                                                       >  2:1588216/1‑93 (MQ=255)

                                     aaGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATc                                                    >  2:2404929/1‑93 (MQ=255)

                                     aaGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATc                                                    <  2:4775510/93‑1 (MQ=255)

                                     aaGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATc                                                    >  2:5993440/1‑93 (MQ=255)

                                      aGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACTCGGGCTATCTCACGCCACCTAATGTTATCt                                                   >  1:3512376/1‑93 (MQ=255)

                                      aGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCt                                                   >  1:5597825/1‑93 (MQ=255)

                                       gCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTg                                                  >  1:668236/1‑93 (MQ=255)

                                       gCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTg                                                  >  2:5085414/1‑93 (MQ=255)

                                        cTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTgg                                                 <  1:6041475/93‑1 (MQ=255)

                                         tGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTggg                                                <  2:3889397/93‑1 (MQ=255)

                                             gATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACg                                            >  2:1203962/1‑93 (MQ=255)

                                              aTGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACgg                                           <  2:2553152/93‑1 (MQ=255)

                                              aTGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACgg                                           <  2:4182984/93‑1 (MQ=255)

                                               tGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGc                                          <  1:4385773/93‑1 (MQ=255)

                                               tGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGc                                          >  2:4873317/1‑93 (MQ=255)

                                                ggATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCt                                         >  1:5527210/1‑93 (MQ=255)

                                                ggATATGGAAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCt                                         >  1:3299375/1‑93 (MQ=255)

                                                 gATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTg                                        >  2:5683095/1‑93 (MQ=255)

                                                 gATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTg                                        <  2:3305759/93‑1 (MQ=255)

                                                 gATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTg                                        >  1:2605970/1‑93 (MQ=255)

                                                   tatGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGc                                      >  1:6141998/1‑93 (MQ=255)

                                                     tGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCag                                    >  1:1952823/1‑93 (MQ=255)

                                                      ggCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCaga                                   <  1:4022584/93‑1 (MQ=255)

                                                      ggCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCaga                                   >  1:3959969/1‑93 (MQ=255)

                                                         aaaaGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGGTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAGaa                                >  1:84716/1‑93 (MQ=255)

                                                         aaaaGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAGaa                                >  1:5646551/1‑93 (MQ=255)

                                                          aaaGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAGAAc                               <  2:4166092/93‑1 (MQ=255)

                                                          aaaGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAGAAc                               <  2:6155267/93‑1 (MQ=255)

                                                           aaGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCGCCTAATGTTATCTGGGGACGGCTGGCAGAGAAcc                              <  2:1828638/93‑1 (MQ=255)

                                                           aaGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAGAAct                              >  2:890146/1‑92 (MQ=255)

                                                           aaGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAGAAcc                              >  1:4776896/1‑93 (MQ=255)

                                                           aaGAGATGAACGCGGGTTATACGCTGATTTCAG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGCGGAcc                              >  1:290737/1‑93 (MQ=255)

                                                            agagATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAGAACCt                             >  2:5268359/1‑93 (MQ=255)

                                                            agagATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAGAACCt                             >  1:308592/1‑93 (MQ=255)

                                                              agaTGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAGAACCTaa                           <  2:2936125/93‑1 (MQ=255)

                                                              agaTGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAGAACCTaa                           <  1:4822524/93‑1 (MQ=255)

                                                              agaTGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAGAACCTaa                           <  1:3656197/93‑1 (MQ=255)

                                                               gaTGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAGAACCTAAg                          <  2:3681425/93‑1 (MQ=255)

                                                                aTGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAGAACCTAAGc                         >  2:3380641/1‑93 (MQ=255)

                                                                   aaCGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAGAACCTAAGCGaa                      >  2:447941/1‑93 (MQ=255)

                                                                    aCGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAGAACCTAAGCGAAc                     <  1:4730552/93‑1 (MQ=255)

                                                                    aCGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAGAACCTAAGCGAAc                     >  1:3738518/1‑93 (MQ=255)

                                                                    aCGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCAGGCTATCTCACCCCACCTAATGTTATCTGGGGACGGCTGGCAGAGAACCTAAGCGCAc                     >  1:758424/1‑93 (MQ=255)

                                                                     cgcgGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAGAACCTAAGCGAACt                    <  2:640609/93‑1 (MQ=255)

                                                                     cgcgGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAGAACCTAAGCGAACt                    <  2:1472592/93‑1 (MQ=255)

                                                                        gggTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAGAACCTAAGCGAACtgtg                 <  2:4288124/93‑1 (MQ=255)

                                                                         ggTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAGAACCTAAGCGAACtgtgt                <  1:4358770/93‑1 (MQ=255)

                                                                         ggTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAGAACCTAAGCGAACtgtgt                <  2:1691796/93‑1 (MQ=255)

                                                                          gTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAGAACCTAAGCGAACtgtgtg               <  2:4112883/93‑1 (MQ=255)

                                                                          gTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAGAACCTAAGCGAACtgtgtg               <  2:1947617/93‑1 (MQ=255)

                                                                           ttATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAGAACCTAAGCGAACTGTGTGa              <  1:2169642/93‑1 (MQ=255)

                                                                            tataCGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAGAACCTAAGCGAACTGTGTGAg             >  1:415791/1‑93 (MQ=255)

                                                                             ataCGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAGAACCTAAGCGAACTGTGTGAGt            <  1:3229759/93‑1 (MQ=255)

                                                                             ataCGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAGAACCTAAGCGAACTGTGTGAGt            <  1:1031684/93‑1 (MQ=255)

                                                                              taCGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAGAACCTAAGCGAACTGTGTGAGTa           <  1:5710913/93‑1 (MQ=255)

                                                                              taCGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAGAACCTAAGCGAACTGTGTGAGTa           <  2:3295403/93‑1 (MQ=255)

                                                                                cGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAGAACCTAAGCGAACTGTGTGAGTAcg         >  2:5915180/1‑93 (MQ=255)

                                                                                cGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAGAACCTAAGCGAACTGTGTGAGTAcg         <  1:6378090/93‑1 (MQ=255)

                                                                                   tGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAGAACCTAAGCGAACTGTGTGAGTACGCgg      >  1:5184091/1‑93 (MQ=255)

                                                                                     aTTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAGAACCTAAGCGAACTGTGTGAGTACGCGGaa    >  2:5810896/1‑93 (MQ=255)

                                                                                      tttCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGCTATCTGGGGACGGCTGGCAGAGAACCTAAGCGAACTGTGCGAGTACGCGGaaa   >  1:6020185/1‑93 (MQ=255)

                                                                                       ttCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAGAACCTAAGCGAACTGTGTGAGTACGCGGaaaa  <  2:1285141/93‑1 (MQ=255)

CGATGAACATATGCGTCGCGAAAGCCTCGACATGATCAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCGCCGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAGAACCTAAGCGAACTGTGTGAGTACGCGGAAAA  >  NC_000913/3500216‑3500396

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CGATGAACATATGCGTCGCGAAAGCCTCGACATGATCAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCGCCGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAGAACCTAAGCGAACTGTGTGAGTACGCGGAAAACATC  >  NC_000913/3500216‑3500400

CGATGAACATATGCGTCGCGAAAGCCTCGACATGATCAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCGCG                                                                                             >  SRR4302534.1331216/1‑94 (MQ=60)

 GATGCACATATGCGTCGCGAAAGCCTCGACATGATCAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCGCGG                                                                                            >  SRR4302534.5474314/1‑94 (MQ=60)

 GATGAACATATGCGTCGCGAAAGCCTCGACATGATCAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCGCGG                                                                                            >  SRR4302534.6425684/1‑94 (MQ=60)

 GATGAACATATGCGTCGCGAAAGCCTCGACATGATCAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCGCGG                                                                                            >  SRR4302534.3032982/1‑94 (MQ=60)

 GATGAACATATGCGTCGCGAAAGCCTCGACATGATCAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCGCGG                                                                                            <  SRR4302534.1535428/94‑1 (MQ=60)

 GATGAACATATGCGTCGCGAAAGCCTCGACATGATCAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCGCGG                                                                                            <  SRR4302534.5223884/94‑1 (MQ=60)

 GATGAACATATGCGTCGCGAAAGCCTCGACATGATCAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCGCGG                                                                                            >  SRR4302534.510510/1‑94 (MQ=60)

   TGAACATATGCGTCGCGAAAGCCTCGACATGATCAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCC                                                                                         >  SRR4302534.475453/1‑94 (MQ=60)

    GAACATATGCGTCGCGAAAGCCTCGACATGATCAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCC                                                                                        >  SRR4302534.1409682/1‑94 (MQ=60)

    GAACAGATGCTTCGCGAAAGCCTCGACATGATCAAGCTGGCGATGGATATGGCAAAAGACATAATCGTTGGTTATAGGCTGATTTCCG‑CGGCCC                                                                                        <  SRR4302534.493884/94‑1 (MQ=52)

     AACATATGCGTCGCGAAAGCCTCGACATGATCAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCGCGGGCCA                                                                                        >  SRR4302534.1690928/1‑94 (MQ=60)

       CATATGCGTCGCGAAAGCCTCGACATGATCAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACG                                                                                     >  SRR4302534.6285433/1‑94 (MQ=60)

        ATATGCGTCGCGAAAGCCTCGACATGATCAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGC                                                                                    >  SRR4302534.398446/1‑94 (MQ=60)

        ATATGCGTCGCGAAAGCCTCGACATGATCAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGC                                                                                    >  SRR4302534.6467173/1‑94 (MQ=60)

        ATATGCGTCGCGAAAGCCTCGACATGATCAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATGTCCG‑CGGCCCACGG                                                                                    >  SRR4302534.6385234/1‑94 (MQ=60)

         TATGCGTCGCGAAAGCCTCGACATGATCAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCG                                                                                   <  SRR4302534.6186763/94‑1 (MQ=60)

          ATGCGTCGCGAAAGCCTCGACATGATCAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGG                                                                                  <  SRR4302534.1350545/94‑1 (MQ=60)

             CGTCGCGAAAGCCTCGACATGATCAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCT                                                                               <  SRR4302534.3254660/94‑1 (MQ=60)

              GTCGCGAAAGCCTCGACATGATCAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTA                                                                              <  SRR4302534.3096540/94‑1 (MQ=60)

              CTCGCGAAAGCCTCGACATGATCAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTA                                                                              <  SRR4302534.2303212/94‑1 (MQ=60)

               TCGCGAAAGCCTCGACATGAACAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGCTTTCCG‑CGGCCCACGCGGGCTAT                                                                             >  SRR4302534.6409238/1‑94 (MQ=60)

                 GCGAAAGCCTCGACATGATCAAGCTGGCGATGTATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCT                                                                           <  SRR4302534.2138306/94‑1 (MQ=60)

                 GCGAAAGCCTCGACATGATCAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCT                                                                           <  SRR4302534.931798/94‑1 (MQ=60)

                  CGAAAGCCTCGACATGATCAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTC                                                                          >  SRR4302534.5546576/1‑94 (MQ=60)

                  CGAAAGCCTCGACATGATCAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTC                                                                          >  SRR4302534.3257356/1‑94 (MQ=60)

                  CGAAAGCCTCGACATGATCAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTC                                                                          <  SRR4302534.4129311/94‑1 (MQ=60)

                  CGAAAGCCTCGACATGATCAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTC                                                                          <  SRR4302534.1428962/94‑1 (MQ=60)

                  CGAAAGCCTCGACATGATCAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTC                                                                          >  SRR4302534.4906561/1‑94 (MQ=60)

                  CGAAAGCCTCGACATGATCAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTC                                                                          >  SRR4302534.3985084/1‑94 (MQ=60)

                   GAAAGCCTCGACATGATCAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCA                                                                         >  SRR4302534.3552629/1‑94 (MQ=60)

                   GAAAGCCTCGACATGATCAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCA                                                                         >  SRR4302534.2838572/1‑94 (MQ=60)

                   GAAAGCCTCGACATGATCAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCA                                                                         >  SRR4302534.94880/1‑94 (MQ=60)

                   GAAAGCCTCGACATGATCAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCCGGCTATCTCA                                                                         >  SRR4302534.1278049/1‑94 (MQ=60)

                     AAGCCTCGACATGATCAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACG                                                                       <  SRR4302534.378125/94‑1 (MQ=60)

                       GCCTCGACATGATCAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCC                                                                     <  SRR4302534.2844000/94‑1 (MQ=60)

                        CCTCGACATGATCAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCA                                                                    >  SRR4302534.6349826/1‑94 (MQ=60)

                           CGACATGATCAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTTACGCCACCT                                                                 <  SRR4302534.6468851/94‑1 (MQ=60)

                           CGACATGATCAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCT                                                                 >  SRR4302534.2393795/1‑94 (MQ=60)

                           CGACATGATCAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCT                                                                 <  SRR4302534.5125101/94‑1 (MQ=60)

                            GACATGATCAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTA                                                                <  SRR4302534.1009047/94‑1 (MQ=60)

                            GACATGATCAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTA                                                                >  SRR4302534.76125/1‑94 (MQ=60)

                               ATGATCAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATG                                                             >  SRR4302534.3542443/1‑94 (MQ=60)

                               ATGATCAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATG                                                             <  SRR4302534.3532813/94‑1 (MQ=60)

                                 GATCAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTT                                                           >  SRR4302534.4896768/1‑94 (MQ=60)

                                 GATCAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTT                                                           >  SRR4302534.2006526/1‑94 (MQ=60)

                                 GATCAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTT                                                           <  SRR4302534.4756985/94‑1 (MQ=60)

                                  ATCAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTA                                                          >  SRR4302534.6532327/1‑94 (MQ=60)

                                    CAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATC                                                        >  SRR4302534.2232993/1‑94 (MQ=60)

                                    CAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATC                                                        >  SRR4302534.4961247/1‑94 (MQ=60)

                                     AAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACTCGGGCTATCTCACGCCACCTAATGTTATCT                                                       >  SRR4302534.3606863/1‑94 (MQ=60)

                                     AAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCT                                                       >  SRR4302534.823885/1‑94 (MQ=60)

                                     AAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCT                                                       >  SRR4302534.5749195/1‑94 (MQ=60)

                                      AGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTG                                                      >  SRR4302534.684516/1‑94 (MQ=60)

                                      AGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGATATCTG                                                      >  SRR4302534.3163681/1‑94 (MQ=60)

                                       GCTGGCGAGGGATATGGCAAAAGACATGAACGCGGGTTATACGCTGCTTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGG                                                     <  SRR4302534.6258156/94‑1 (MQ=60)

                                        CTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGG                                                    >  SRR4302534.2056492/1‑94 (MQ=60)

                                        CTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGG                                                    <  SRR4302534.6205688/94‑1 (MQ=60)

                                        CTGACGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGG                                                    >  SRR4302534.5856411/1‑94 (MQ=60)

                                         TGGCGATGGATATGGCAAAAGAGATGAACGCGTGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGG                                                   <  SRR4302534.6633718/94‑1 (MQ=60)

                                            CGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACG                                                >  SRR4302534.3888748/1‑94 (MQ=60)

                                              ATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGC                                              <  SRR4302534.869167/94‑1 (MQ=60)

                                              ATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGC                                              >  SRR4302534.2809329/1‑94 (MQ=60)

                                              ATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGC                                              >  SRR4302534.4915738/1‑94 (MQ=60)

                                              ATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGC                                              >  SRR4302534.1355663/1‑94 (MQ=60)

                                               TGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCT                                             >  SRR4302534.5676380/1‑94 (MQ=60)

                                               TGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCT                                             <  SRR4302534.4504019/94‑1 (MQ=60)

                                               TGGATATGGAAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCT                                             >  SRR4302534.3387434/1‑94 (MQ=60)

                                                GGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTG                                            <  SRR4302534.4332204/94‑1 (MQ=60)

                                                GGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTG                                            >  SRR4302534.2674920/1‑94 (MQ=60)

                                                 GATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGG                                           <  SRR4302534.5527722/94‑1 (MQ=60)

                                                  ATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGC                                          >  SRR4302534.6309047/1‑94 (MQ=60)

                                                    ATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAG                                        >  SRR4302534.2003929/1‑94 (MQ=60)

                                                     TGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGA                                       >  SRR4302534.4066000/1‑94 (MQ=60)

                                                      GGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAG                                      >  SRR4302534.399749/1‑94 (MQ=60)

                                                      GGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAG                                      <  SRR4302534.4130439/94‑1 (MQ=60)

                                                        CAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGGTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAGAA                                    >  SRR4302534.86975/1‑94 (MQ=60)

                                                        CAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAGAA                                    <  SRR4302534.1423062/94‑1 (MQ=60)

                                                        CAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAGAA                                    >  SRR4302534.5799395/1‑94 (MQ=60)

                                                        CAAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAGAA                                    <  SRR4302534.4434077/94‑1 (MQ=60)

                                                         AAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACatccatcgccagcttgatcatgtcgaggcttt                                   <  SRR4302534.2114628/94‑33 (MQ=60)

                                                         AAAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAGAAC                                   >  SRR4302534.4150078/1‑94 (MQ=60)

                                                          AAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAGAACC                                  >  SRR4302534.4906614/1‑94 (MQ=60)

                                                          AAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAGAACC                                  >  SRR4302534.369894/1‑94 (MQ=60)

                                                          AAAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAGAACC                                  >  SRR4302534.4484081/1‑94 (MQ=60)

                                                          AAAGAGATGAACGCGGGTTATACGCTGATTTCAG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGCGGACC                                  >  SRR4302534.298256/1‑94 (MQ=60)

                                                           AAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAGAACCT                                 >  SRR4302534.6428983/1‑94 (MQ=60)

                                                           AAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAGAACCT                                 >  SRR4302534.316566/1‑94 (MQ=60)

                                                           AAGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAGAACCT                                 >  SRR4302534.2855635/1‑94 (MQ=60)

                                                            AGAGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAGAACCTA                                <  SRR4302534.3603021/94‑1 (MQ=60)

                                                            AGAGATGAAAGCGGGGTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAGAACCTA                                >  SRR4302534.5867274/1‑94 (MQ=60)

                                                              AGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAGAACCTAAG                              >  SRR4302534.1938156/1‑94 (MQ=60)

                                                              AGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAGAACCTAAG                              <  SRR4302534.4953400/94‑1 (MQ=60)

                                                              AGATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAGAACCTAAG                              <  SRR4302534.3754465/94‑1 (MQ=60)

                                                              AGATGAACGCGGGTTATACGCTGAATTCCGCC‑GCCCACGCGTGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAGAACCTACG                              <  SRR4302534.711499/94‑1 (MQ=60)

                                                               GATGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAGAACCTAAGC                             <  SRR4302534.5734533/94‑1 (MQ=60)

                                                                 TGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAGAACCTAAGCGA                           >  SRR4302534.5237202/1‑94 (MQ=60)

                                                                 TGAACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAGAACCTAAGCGA                           >  SRR4302534.3024769/1‑94 (MQ=60)

                                                                   AACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAGAACCTAAGCGAAC                         >  SRR4302534.6222460/1‑94 (MQ=60)

                                                                   AACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAGAACCTAAGCGAAC                         >  SRR4302534.5148043/1‑94 (MQ=60)

                                                                   AACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAGAACCTAAGCGAAC                         >  SRR4302534.3838952/1‑94 (MQ=60)

                                                                   AACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCAGGCTATCTCACCCCACCTAATGTTATCTGGGGACGGCTGGCAGAGAACCTAAGCGCAC                         >  SRR4302534.777235/1‑94 (MQ=60)

                                                                    ACGCGGGTTATACGCTGATTTCCG‑CGGCCTACGCAGGCTATCGCACGCCGCCTAATGTTATCTGGGGACGGCTTGCAGAGAACCTAAGCGAACT                        <  SRR4302534.972671/94‑1 (MQ=60)

                                                                    ACGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAGAACCTAAGCGAACT                        <  SRR4302534.4859016/94‑1 (MQ=60)

                                                                     CGCGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAGAACCTAAGCGAACTG                       >  SRR4302534.2406433/1‑94 (MQ=60)

                                                                      GCGGGTTATGCGCGGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAGAACCTAAGCGAACTGT                      <  SRR4302534.4617221/94‑1 (MQ=60)

                                                                       CGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAGAACCTAAGCGAACTGTG                     >  SRR4302534.3335887/1‑94 (MQ=60)

                                                                       CGGGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAGAACCTAAGCGAACTGTG                     <  SRR4302534.4127009/94‑1 (MQ=60)

                                                                         GGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAGAACCTAAGCGAACTGTGTG                   <  SRR4302534.4476369/94‑1 (MQ=60)

                                                                         GGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAGAACCTAAGCGAACTGTGTG                   <  SRR4302534.6338878/94‑1 (MQ=60)

                                                                         GGTTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAGAACCTAAGCGAACTGTGTG                   <  SRR4302534.6338874/94‑1 (MQ=60)

                                                                           TTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAGAACCTAAGCGAACTGTGTGAG                 <  SRR4302534.2226950/94‑1 (MQ=60)

                                                                           TTATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAGAACCTAAGCGAACTGTGTGAG                 >  SRR4302534.426412/1‑94 (MQ=60)

                                                                            TATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAGAACCTAAGCGAACTGTGTGAGA                >  SRR4302534.5754278/1‑94 (MQ=60)

                                                                             ATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAGAACCTAAGCGAACTGTGTGAGTA               <  SRR4302534.1058271/94‑1 (MQ=60)

                                                                             ATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAGAACCTAAGCGAACTGTGTGAGTA               <  SRR4302534.2181771/94‑1 (MQ=60)

                                                                             ATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAGAACCTAAGCGAACTGTGTGAGTA               >  SRR4302534.810633/1‑94 (MQ=60)

                                                                             ATACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAGAACCTAAGCGAACTGTGTGAGTA               <  SRR4302534.3315585/94‑1 (MQ=60)

                                                                              TACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAGAACCTAAGCGCACTGTGTGAGTAG              >  SRR4302534.6015119/1‑94 (MQ=60)

                                                                              TACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAGAACCTAAGCGAACTGTGTGAGTAC              <  SRR4302534.5865628/94‑1 (MQ=60)

                                                                               ACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAGAACCTAAGCGAACTGTGTGAGTACG             <  SRR4302534.5274042/94‑1 (MQ=60)

                                                                               ACGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAGAACCTAAGCGAACTGTGTGAGTACG             >  SRR4302534.4613126/1‑94 (MQ=60)

                                                                                CGCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAGAACCTAAGCGAACTGTGTGAGTACGC            <  SRR4302534.6550360/94‑1 (MQ=60)

                                                                                 GCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAGAACCTAAGCGAACTGTGTGAGTACGCG           <  SRR4302534.6307421/94‑1 (MQ=60)

                                                                                 GCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAGAACCTAAGCGAACTGTGTGAGTACGCG           <  SRR4302534.1560373/94‑1 (MQ=60)

                                                                                 GCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAGAACCTAAGCGAACTGTGTGAGTACGCG           >  SRR4302534.6557005/1‑94 (MQ=60)

                                                                                 GCTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAGAACCTAAGCGAACTGTGTGAGTACGCG           >  SRR4302534.3669288/1‑94 (MQ=60)

                                                                                  CTGATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAGAACCTAAGCGAACTGTGTGAGTACGCGG          >  SRR4302534.5323819/1‑94 (MQ=60)

                                                                                   TGATTTCCG‑CGGCCCACGCGGACTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAGAACCTAAGCGAACTGTGTGAGTACGCGGA         >  SRR4302534.5394302/1‑94 (MQ=60)

                                                                                     ATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAGAACCTAAGCGAACTGTGTGAGTACGCGGAAA       >  SRR4302534.5752341/1‑94 (MQ=60)

                                                                                     ATTTCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGCTATCTGGGGACGGCTGGCAGAGAACCTAAGCGAACTGTGCGAGTACGCGGAAA       >  SRR4302534.6183786/1‑94 (MQ=60)

                                                                                        TCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAGAACCTAAGCGAACTGTGTGAGTACGCGGAAAACA    <  SRR4302534.6531472/94‑1 (MQ=60)

                                                                                        TCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAGAACCTAAGCGAACTGTGTGAGTACGCGGAAAACA    <  SRR4302534.2868598/94‑1 (MQ=60)

                                                                                        TCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAGAACCTAAGCGAACTGTGTGAGTACGCGGAAAACA    <  SRR4302534.2735540/94‑1 (MQ=60)

                                                                                        TCCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAGAACCTAAGCGAACTGTGTGAGTACGCGGAAAACA    <  SRR4302534.561530/94‑1 (MQ=60)

                                                                                        TCCG‑CGGCCCACGCGGGCTAGCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAGAACCTAAGCGAACTGTGTGAGTACGCGGAAAACA    <  SRR4302534.5380420/94‑1 (MQ=60)

                                                                                         CCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAGAACCTAAGCGAACTGTGTGAGTACGCGGAAAACAT   <  SRR4302534.2687694/94‑1 (MQ=60)

                                                                                         CCG‑CGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAGAACCTAAGCGAACTGTGTGAGTACGCGGAAAACAT   <  SRR4302534.2638045/94‑1 (MQ=60)

                                                                                           cgCGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAGAACCTAAGCGAACTGTGTGAGTACGCGGAAAACATC  >  SRR4302534.719649/3‑94 (MQ=60)

                                                                                           cgCGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAGAACCTAAGCGAACTGTGTGAGTACGCGGAAAACATC  <  SRR4302534.4447865/92‑1 (MQ=60)

CGATGAACATATGCGTCGCGAAAGCCTCGACATGATCAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCGCCGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAGAACCTAAGCGAACTGTGTGAGTACGCGGAAAACATC  >  NC_000913/3500216‑3500400

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`