Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 884 257.2 12958910 96.9% 12557183 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,714,436 2 bp→GC coding (261‑262/660 nt) yiaD → putative outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,714,4360CG100.0% 771.3 / NA 226N87K (AAC→AAG) yiaDputative outer membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (102/124);  total (102/124)
*NC_0009133,714,4370GC100.0% 764.3 / NA 226V88L (GTG→CTG) yiaDputative outer membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (102/124);  total (102/124)

Only 100 of 236 total aligned reads displayed.

CGCGGTAAAGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAACGTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCTTC  >  NC_000913/3714350‑3714523
                                                                                      ||                                                                                      
   ggTAAAGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGAc                                                                                <  2:2921784/93‑1 (MQ=255)
     tAAAGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACaa                                                                              >  1:2483046/1‑93 (MQ=255)
       aaGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACaaaa                                                                            >  1:6093533/1‑93 (MQ=255)
       aaGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACaaaa                                                                            <  1:2077342/93‑1 (MQ=255)
        aGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAAt                                                                           >  1:1578259/1‑93 (MQ=255)
        aGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAAt                                                                           >  2:5943645/1‑93 (MQ=255)
          gcgcgcTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATgc                                                                         <  2:4723442/93‑1 (MQ=255)
          gcgcgcTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATgc                                                                         <  2:3662231/93‑1 (MQ=255)
          gcgcgcTGATTGGCGCAGCAGCAGGAGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATgc                                                                         >  1:4256065/1‑93 (MQ=255)
           cgcgcTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATgcg                                                                        <  2:1841960/93‑1 (MQ=255)
           cgcgcTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATgcg                                                                        <  2:4303347/93‑1 (MQ=255)
            gcgcTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATgcgc                                                                       >  2:4209750/1‑93 (MQ=255)
             cgcTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGcgcg                                                                      <  1:730924/93‑1 (MQ=255)
             cgcTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGcgcg                                                                      >  2:5389954/1‑93 (MQ=255)
             cgcTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGcgcg                                                                      <  1:3153371/93‑1 (MQ=255)
              gcTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCgg                                                                     >  2:1874187/1‑93 (MQ=255)
               cTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGc                                                                    >  2:6178011/1‑93 (MQ=255)
                 gATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCAc                                                                  <  1:958862/93‑1 (MQ=255)
                 gATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCAc                                                                  <  2:1228524/93‑1 (MQ=255)
                   ttGGCGCAGCAGCAGGAGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTg                                                                >  1:4383311/1‑93 (MQ=255)
                    tGGCGCAGCAGCAGGCGCAGCTCTGGGGGGGGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTgg                                                               <  1:1603793/93‑1 (MQ=255)
                     ggCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGgt                                                              >  1:2715154/1‑93 (MQ=255)
                     ggCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGgt                                                              >  2:3584198/1‑93 (MQ=255)
                      gcgcAGCATCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACCAAATGCGCGGCACTGgtg                                                             >  1:6315137/1‑93 (MQ=255)
                      gcgcAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGgtg                                                             <  2:2687745/93‑1 (MQ=255)
                       cgcAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGgtgt                                                            <  2:1058630/93‑1 (MQ=255)
                        gcagcagcaGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGtt                                                           >  2:2732281/1‑93 (MQ=255)
                        gcagcagcaGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGtt                                                           <  1:3896675/93‑1 (MQ=255)
                        gcagcagcaGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGtt                                                           <  2:426380/93‑1 (MQ=255)
                         cagcagcagGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTa                                                          >  2:2520219/1‑93 (MQ=255)
                          agcagcagGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAg                                                         <  2:2211996/93‑1 (MQ=255)
                          agcagcagGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAg                                                         >  1:5095066/1‑93 (MQ=255)
                           ggagcagGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGGCAAAATGCGCGGCACTGGTGTTAGc                                                        >  2:6244420/3‑93 (MQ=255)
                           gcagcagGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGc                                                        >  1:5544360/1‑93 (MQ=255)
                            cagcagGCGCAGCTCTGGGCTGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCg                                                       <  2:6409775/93‑1 (MQ=255)
                            cagcagGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCg                                                       >  2:909102/1‑93 (MQ=255)
                             agcagGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGt                                                      <  2:4379213/93‑1 (MQ=255)
                              gcagGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTa                                                     >  1:1388726/1‑93 (MQ=255)
                              gcagGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTa                                                     >  2:5458592/1‑93 (MQ=255)
                              gcagGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTa                                                     <  2:2621025/93‑1 (MQ=255)
                                agGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAAc                                                   <  1:4647654/93‑1 (MQ=255)
                                agGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAAc                                                   >  1:3559148/1‑93 (MQ=255)
                                 ggCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAAcc                                                  >  1:1017132/1‑93 (MQ=255)
                                 ggCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAAcc                                                  <  2:3879476/93‑1 (MQ=255)
                                 ggCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAAcc                                                  <  2:3768558/93‑1 (MQ=255)
                                     cAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCa                                              <  1:5192681/93‑1 (MQ=255)
                                      aGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAg                                             <  1:5367264/93‑1 (MQ=255)
                                      aGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAg                                             >  1:832777/1‑93 (MQ=255)
                                      aGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAg                                             <  2:1299658/93‑1 (MQ=255)
                                       gCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGc                                            >  1:5467834/1‑93 (MQ=255)
                                       gCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGc                                            >  2:5623097/1‑93 (MQ=255)
                                        ctctGGGCGGCGGCGTTGGTTATTACATGGATNTGNAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCg                                           <  1:4862608/93‑1 (MQ=255)
                                        ctctGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCg                                           >  2:5846228/1‑93 (MQ=255)
                                        ctctGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCg                                           <  1:3224740/93‑1 (MQ=255)
                                         tctGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCgg                                          <  2:4555238/93‑1 (MQ=255)
                                         tctGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCgg                                          <  1:1716087/93‑1 (MQ=255)
                                         tctGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCgg                                          >  2:2165059/1‑93 (MQ=255)
                                           tGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCgggg                                        >  1:2972276/1‑93 (MQ=255)
                                            gggCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGa                                       <  1:1100268/93‑1 (MQ=255)
                                             ggcggcggcGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGAt                                      <  2:3797263/93‑1 (MQ=255)
                                             ggcggcggcGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGAt                                      <  1:3300508/93‑1 (MQ=255)
                                             ggcggcggcGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGAt                                      <  1:2930490/93‑1 (MQ=255)
                                              gcggcggcgTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATa                                     <  2:3166985/93‑1 (MQ=255)
                                               cggcggcgTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATaa                                    >  2:5890830/1‑93 (MQ=255)
                                                 gcggcgTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACa                                  <  1:1706470/93‑1 (MQ=255)
                                                 gcggcgTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACa                                  <  1:1206216/93‑1 (MQ=255)
                                                 gcggcgTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACa                                  >  1:4838706/1‑93 (MQ=255)
                                                   ggcgTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACAtt                                <  1:4131155/93‑1 (MQ=255)
                                                   ggcgTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACAtt                                <  1:3271036/93‑1 (MQ=255)
                                                    gcgTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTa                               >  2:1882754/1‑93 (MQ=255)
                                                    gcgTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTa                               >  2:4989404/1‑93 (MQ=255)
                                                    gcgTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTa                               >  2:169756/1‑93 (MQ=255)
                                                       ttGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATcc                            <  2:4886140/93‑1 (MQ=255)
                                                         ggTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCta                          >  2:5301305/1‑92 (MQ=255)
                                                         ggTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTc                          >  1:386753/1‑93 (MQ=255)
                                                          gTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCa                         <  2:3577518/93‑1 (MQ=255)
                                                           ttattaCATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCaa                        <  1:643473/93‑1 (MQ=255)
                                                            tattaCATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAat                       <  1:2107473/93‑1 (MQ=255)
                                                            tattaCATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAat                       >  2:4791425/1‑93 (MQ=255)
                                                              ttaCATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAatat                     <  1:348318/93‑1 (MQ=255)
                                                                 cATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGcc                  >  2:3987711/1‑93 (MQ=255)
                                                                  aTGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCg                 >  1:3983031/1‑93 (MQ=255)
                                                                      aTGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACa             >  1:5808400/1‑93 (MQ=255)
                                                                       tgtgCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACaa            <  1:5708695/93‑1 (MQ=255)
                                                                       tgtgCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACaa            <  1:2443881/93‑1 (MQ=255)
                                                                          gCAGAAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATCCCGAACAAtgt         <  2:6106147/93‑1 (MQ=255)
                                                                           cAGGAAGCGACGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAAtgtg        <  1:6244788/93‑1 (MQ=255)
                                                                           cAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAAtgtg        <  1:2223694/93‑1 (MQ=255)
                                                                             ggAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGAc      >  1:1834839/1‑93 (MQ=255)
                                                                             ggAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGAc      <  2:684996/93‑1 (MQ=255)
                                                                             ggAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGAc      >  2:3201951/1‑93 (MQ=255)
                                                                              gaagcgaagcTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGAcc     >  2:667226/1‑93 (MQ=255)
                                                                              gaagcgaagcTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGAcc     >  1:2096657/1‑93 (MQ=255)
                                                                               aaGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCt    >  2:2370300/1‑93 (MQ=255)
                                                                               aaGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCt    >  2:5175333/1‑93 (MQ=255)
                                                                               aaGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCt    <  2:4658431/93‑1 (MQ=255)
                                                                                aGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCtt   <  2:3367700/93‑1 (MQ=255)
                                                                                aGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCtt   >  1:698515/1‑93 (MQ=255)
                                                                                 gCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCTTc  >  2:805224/1‑93 (MQ=255)
                                                                                 gCGAAGATGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCTTc  <  1:2204934/93‑1 (MQ=255)
                                                                                      ||                                                                                      
CGCGGTAAAGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAACGTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCTTC  >  NC_000913/3714350‑3714523

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATCGCGGTAAAGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAACGTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCTTCGA  >  NC_000913/3714348‑3714525
                                                                                        |                                                                                         
ATCGCGGTAAAGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCG                                                                                      <  SRR4302534.892944/94‑1 (MQ=60)
 TCGCGGTAAAGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGC                                                                                     <  SRR4302534.4673553/94‑1 (MQ=60)
  CGCGGTAGAGTCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCG                                                                                    <  SRR4302534.6107821/94‑1 (MQ=60)
  CGCGGTAAAGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCG                                                                                    <  SRR4302534.4803688/94‑1 (MQ=60)
  CGCGGTAAAGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCG                                                                                    <  SRR4302534.3874081/94‑1 (MQ=60)
      GTAAAGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAA                                                                                >  SRR4302534.2548987/1‑94 (MQ=60)
        AAAGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAA                                                                              >  SRR4302534.6259233/1‑94 (MQ=60)
         AAGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAAT                                                                             <  SRR4302534.2131727/94‑1 (MQ=60)
         AAGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAAT                                                                             <  SRR4302534.2547979/94‑1 (MQ=60)
         AAGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAAT                                                                             >  SRR4302534.1619555/1‑94 (MQ=60)
         AAGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAAT                                                                             <  SRR4302534.4349602/94‑1 (MQ=60)
         AAGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGCATTTGCAGGAAGCGAAGCTGCGCGACAAAAT                                                                             >  SRR4302534.4430406/1‑94 (MQ=60)
          AGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATG                                                                            <  SRR4302534.807400/94‑1 (MQ=60)
          AGGCGCGCTGATTGGCGCAGCAGCAGGAGCAGCTCTGGGCGGCGGCGTTGGTTATTACGTGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATG                                                                            >  SRR4302534.4172635/1‑94 (MQ=60)
           GGCGCGCTGATTGGCGCAGCAGCAGGAGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGC                                                                           >  SRR4302534.4370942/1‑94 (MQ=60)
            GCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCG                                                                          <  SRR4302534.95491/94‑1 (MQ=60)
              GCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCG                                                                        <  SRR4302534.1079802/94‑1 (MQ=60)
              GCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCG                                                                        >  SRR4302534.1241315/1‑94 (MQ=60)
              GCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCG                                                                        <  SRR4302534.1560521/94‑1 (MQ=60)
               CGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGG                                                                       <  SRR4302534.748983/94‑1 (MQ=60)
               CGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGG                                                                       >  SRR4302534.2224649/1‑94 (MQ=60)
               CGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGG                                                                       <  SRR4302534.3236411/94‑1 (MQ=60)
                GCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGC                                                                      >  SRR4302534.3334896/1‑94 (MQ=60)
                 CTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCA                                                                     >  SRR4302534.4189093/1‑94 (MQ=60)
                  TGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCAC                                                                    >  SRR4302534.5124880/1‑94 (MQ=60)
                   GATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACT                                                                   <  SRR4302534.983290/94‑1 (MQ=60)
                   GATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACT                                                                   <  SRR4302534.3704757/94‑1 (MQ=60)
                    ATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTG                                                                  <  SRR4302534.4473185/94‑1 (MQ=60)
                    ATTGGCGCAGCAGCAGGAGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTG                                                                  >  SRR4302534.4501475/1‑94 (MQ=60)
                      TGGCGCAGCAGCAGGCGCAGCTCTGGGGGGGGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGT                                                                <  SRR4302534.1645689/94‑1 (MQ=60)
                      TGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGT                                                                >  SRR4302534.2786819/1‑94 (MQ=60)
                      TGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGT                                                                >  SRR4302534.3520319/1‑94 (MQ=60)
                       GGCGCAGCATCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACCAAATGCGCGGCACTGGTG                                                               >  SRR4302534.6485828/1‑94 (MQ=60)
                         CGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTT                                                             >  SRR4302534.6366593/1‑94 (MQ=60)
                         CGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTC                                                             <  SRR4302534.6630685/94‑1 (MQ=60)
                          GCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTA                                                            <  SRR4302534.4001248/94‑1 (MQ=60)
                           CAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAG                                                           <  SRR4302534.5407468/94‑1 (MQ=60)
                           CAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAG                                                           >  SRR4302534.5232590/1‑94 (MQ=60)
                            AGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGC                                                          >  SRR4302534.4755471/1‑94 (MQ=60)
                            AGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGC                                                          >  SRR4302534.5694020/1‑94 (MQ=60)
                             GCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTCGTGTTAGCG                                                         >  SRR4302534.3654540/1‑94 (MQ=60)
                               AGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTA                                                       >  SRR4302534.1425273/1‑94 (MQ=60)
                               AGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTA                                                       >  SRR4302534.729169/1‑94 (MQ=60)
                                 CAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAAC                                                     >  SRR4302534.3654910/1‑94 (MQ=60)
                                  AGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACC                                                    <  SRR4302534.4773816/94‑1 (MQ=60)
                                  AGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACC                                                    >  SRR4302534.1043319/1‑94 (MQ=60)
                                   GGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCC                                                   >  SRR4302534.5643129/1‑94 (MQ=60)
                                    GCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCG                                                  >  SRR4302534.4970096/1‑94 (MQ=60)
                                    GCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTACCGTAACCCG                                                  <  SRR4302534.6535445/94‑1 (MQ=60)
                                       CAGCTCTGTGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAG                                               <  SRR4302534.2821242/94‑1 (MQ=60)
                                       CAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAG                                               <  SRR4302534.5332679/94‑1 (MQ=60)
                                       CAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAG                                               >  SRR4302534.3035609/1‑94 (MQ=60)
                                       CAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAG                                               >  SRR4302534.853722/1‑94 (MQ=60)
                                        AGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGC                                              <  SRR4302534.3171215/94‑1 (MQ=60)
                                        AGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGC                                              >  SRR4302534.5615220/1‑94 (MQ=60)
                                        AGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGC                                              >  SRR4302534.1897232/1‑94 (MQ=60)
                                        AGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGC                                              <  SRR4302534.5511921/94‑1 (MQ=60)
                                          CTCTGGGCGGCGGCGTTGGTTATTACATGGATNTGNAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGG                                            <  SRR4302534.4994523/94‑1 (MQ=60)
                                          CTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGG                                            <  SRR4302534.3310396/94‑1 (MQ=60)
                                           TCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGG                                           <  SRR4302534.1755648/94‑1 (MQ=60)
                                           TCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGG                                           <  SRR4302534.1760669/94‑1 (MQ=60)
                                            CTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGG                                          >  SRR4302534.3050098/1‑94 (MQ=60)
                                             TGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGA                                         <  SRR4302534.4005629/94‑1 (MQ=60)
                                              GGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGAT                                        <  SRR4302534.1128936/94‑1 (MQ=60)
                                              GGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGAT                                        >  SRR4302534.1707232/1‑94 (MQ=60)
                                               GGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATA                                       <  SRR4302534.3388600/94‑1 (MQ=60)
                                               GGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATA                                       <  SRR4302534.662341/94‑1 (MQ=60)
                                               GGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATA                                       <  SRR4302534.3007424/94‑1 (MQ=60)
                                                  GGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACA                                    >  SRR4302534.4969957/1‑94 (MQ=60)
                                                   GCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACAT                                   <  SRR4302534.2891791/94‑1 (MQ=60)
                                                   GCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACAT                                   <  SRR4302534.1750801/94‑1 (MQ=60)
                                                   GCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACAT                                   <  SRR4302534.1237956/94‑1 (MQ=60)
                                                   GCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACAT                                   >  SRR4302534.2272499/1‑94 (MQ=60)
                                                    CGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATT                                  <  SRR4302534.5080572/94‑1 (MQ=60)
                                                     GGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTA                                 <  SRR4302534.4242508/94‑1 (MQ=60)
                                                     GGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTA                                 <  SRR4302534.955479/94‑1 (MQ=60)
                                                     GGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTA                                 <  SRR4302534.3358173/94‑1 (MQ=60)
                                                        GTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCC                              <  SRR4302534.1761175/94‑1 (MQ=60)
                                                         TTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCT                             <  SRR4302534.5925871/94‑1 (MQ=60)
                                                          TGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTC                            >  SRR4302534.396646/1‑94 (MQ=60)
                                                           GGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCA                           <  SRR4302534.5832622/94‑1 (MQ=60)
                                                            GTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGAATAACATTATCCTCAA                          <  SRR4302534.3298286/94‑1 (MQ=60)
                                                             TTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAAT                         <  SRR4302534.659088/94‑1 (MQ=60)
                                                              TATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATA                        <  SRR4302534.2162846/94‑1 (MQ=60)
                                                              TATTACATGGATGTGCAGAAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGAAGCGGGGATAACATTATCCTCAATA                        <  SRR4302534.3382881/94‑1 (MQ=60)
                                                               ATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATAT                       >  SRR4302534.1186792/1‑94 (MQ=60)
                                                               ATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTAACCTCAATAT                       <  SRR4302534.6481073/94‑1 (MQ=60)
                                                                TTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATG                      <  SRR4302534.357247/94‑1 (MQ=60)
                                                                 TACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGC                     >  SRR4302534.1635332/1‑94 (MQ=60)
                                                                  TCATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCC                    <  SRR4302534.3514150/94‑1 (MQ=60)
                                                                   CATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCG                   >  SRR4302534.4089626/1‑94 (MQ=60)
                                                                      GGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAAC                >  SRR4302534.5265176/1‑94 (MQ=60)
                                                                       GATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACA               <  SRR4302534.5610035/94‑1 (MQ=60)
                                                                       GATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACA               >  SRR4302534.5966046/1‑94 (MQ=60)
                                                                       GATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACA               >  SRR4302534.3264258/1‑94 (MQ=60)
                                                                       GATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACA               <  SRR4302534.4753728/94‑1 (MQ=60)
                                                                        ATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAA              >  SRR4302534.2158946/1‑94 (MQ=60)
                                                                         TGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAAT             <  SRR4302534.3706570/94‑1 (MQ=60)
                                                                         TGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAAT             <  SRR4302534.5863356/94‑1 (MQ=60)
                                                                         TGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAAT             <  SRR4302534.2508832/94‑1 (MQ=60)
                                                                           TGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGT           >  SRR4302534.3980689/1‑94 (MQ=60)
                                                                           TGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGT           <  SRR4302534.4824954/94‑1 (MQ=60)
                                                                            GCAGGATGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGACCAATGTG          <  SRR4302534.2142144/94‑1 (MQ=60)
                                                                             CAGGAAGCGACGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGA         <  SRR4302534.6414198/94‑1 (MQ=60)
                                                                             CAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGC         >  SRR4302534.2503433/1‑94 (MQ=60)
                                                                             CAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGA         <  SRR4302534.2282582/94‑1 (MQ=60)
                                                                             CAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGA         >  SRR4302534.5596878/1‑94 (MQ=60)
                                                                             CAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGA         <  SRR4302534.1329670/94‑1 (MQ=60)
                                                                             CAGCAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGA         >  SRR4302534.2117662/1‑94 (MQ=60)
                                                                              AGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGAC        >  SRR4302534.2217786/1‑94 (MQ=60)
                                                                              AGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGAC        >  SRR4302534.1882549/1‑94 (MQ=60)
                                                                               GGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACC       >  SRR4302534.2151660/1‑94 (MQ=60)
                                                                               GGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACC       <  SRR4302534.1653346/94‑1 (MQ=60)
                                                                                 AAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCTT     <  SRR4302534.583526/94‑1 (MQ=60)
                                                                                 AAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCTT     >  SRR4302534.715613/1‑94 (MQ=60)
                                                                                  AGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCTTC    <  SRR4302534.2729099/94‑1 (MQ=60)
                                                                                   GCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCTTCG   <  SRR4302534.6307292/94‑1 (MQ=60)
                                                                                   GCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCTTCG   >  SRR4302534.6237821/1‑94 (MQ=60)
                                                                                   GCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCTTCG   <  SRR4302534.2486699/94‑1 (MQ=60)
                                                                                   GCGAAGATGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCTTCG   <  SRR4302534.2263288/94‑1 (MQ=60)
                                                                                    CGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCTTCGA  <  SRR4302534.1971318/94‑1 (MQ=60)
                                                                                        |                                                                                         
ATCGCGGTAAAGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAACGTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCTTCGA  >  NC_000913/3714348‑3714525

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑