Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 884 257.2 12958910 96.9% 12557183 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,734,264 2 bp→GC coding (542‑543/825 nt) bax ← putative ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,734,2640CG99.7% 1169.4 / NA 329S181C (TCG→TGC) baxputative ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base G (175/153);  minor base T (0/1);  total (175/154)
Fisher's exact test for biased strand distribution p-value = 4.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*NC_0009133,734,2650GC99.7% 1158.0 / NA 329S181C (TCG→TGC) baxputative ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base C (174/154);  minor base T (1/0);  total (175/154)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 332 total aligned reads displayed.

GACATAGGCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATCGATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGCCACCATA  >  NC_000913/3734177‑3734351
                                                                                       ||                                                                                      
    tAGGCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATg                                                                                >  2:5685884/1‑93 (MQ=255)
    tAGGCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATg                                                                                >  2:1346958/1‑93 (MQ=255)
         gcTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGaa                                                                           <  1:5556576/93‑1 (MQ=255)
          cTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAAc                                                                          <  1:6244196/93‑1 (MQ=255)
          cTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAAc                                                                          >  2:1432994/1‑93 (MQ=255)
          cTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAAc                                                                          <  1:2404180/93‑1 (MQ=255)
            cACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAg                                                                        <  1:5303622/93‑1 (MQ=255)
             aCCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAgg                                                                       >  1:1651233/1‑93 (MQ=255)
              ccGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGgt                                                                      <  1:4795979/93‑1 (MQ=255)
              ccGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGgt                                                                      <  1:4153642/93‑1 (MQ=255)
                gATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGgttg                                                                    <  2:2449765/93‑1 (MQ=255)
                 aTTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGgttgt                                                                   <  1:5067321/93‑1 (MQ=255)
                 aTTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGgttgt                                                                   >  2:516701/1‑93 (MQ=255)
                  ttCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGgttgtt                                                                  >  2:5993743/1‑93 (MQ=255)
                  ttCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGgttgtt                                                                  >  2:1512854/1‑93 (MQ=255)
                    cTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGTACAGgttgttgt                                                                <  2:3466429/93‑1 (MQ=255)
                    cTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGgttgttgt                                                                <  1:2032220/93‑1 (MQ=255)
                    cTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGgttgttgt                                                                <  1:230609/93‑1 (MQ=255)
                     tttGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGgttgttgtt                                                               <  2:1902529/93‑1 (MQ=255)
                     tttGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGgttgttgtt                                                               >  2:5496391/1‑93 (MQ=255)
                     tttGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGgttgttgtt                                                               <  2:5284893/93‑1 (MQ=255)
                     tttGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTAATGCCGAACAGgttgttgtt                                                               >  1:4281845/1‑93 (MQ=255)
                        gACAGAACTAAACTGTGAGTGCCCTGTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGgttgttgttgtt                                                            <  1:1962236/93‑1 (MQ=255)
                        gACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGgttgttgttgtt                                                            <  2:4242783/93‑1 (MQ=255)
                        gACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGgttgttgttgtt                                                            >  1:1122160/1‑93 (MQ=255)
                        gACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGgttgttgttgtt                                                            >  1:648720/1‑93 (MQ=255)
                        gACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGAGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGgttgttgttgtt                                                            >  2:3812724/1‑93 (MQ=255)
                         aCAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGttgttgttgttg                                                           >  2:2356026/1‑93 (MQ=255)
                          cAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTgc                                                          <  1:3142907/93‑1 (MQ=255)
                          cAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTgc                                                          <  2:3779631/93‑1 (MQ=255)
                            gAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTgcgc                                                        >  1:5509349/1‑93 (MQ=255)
                            gAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTgcgc                                                        <  2:292683/93‑1 (MQ=255)
                            gAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTgcgc                                                        <  2:727263/93‑1 (MQ=255)
                            gAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTgcgc                                                        >  1:1495261/1‑93 (MQ=255)
                               cTAAACTGTGAGTACTCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGTATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGcc                                                     >  1:6084609/1‑93 (MQ=255)
                               cTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGcc                                                     <  1:5864808/93‑1 (MQ=255)
                                tAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCa                                                    <  1:4416714/93‑1 (MQ=255)
                                tAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCa                                                    >  1:2544533/1‑93 (MQ=255)
                                 aaaCTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAg                                                   >  1:4928511/1‑93 (MQ=255)
                                  aaCTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGc                                                  <  2:2186270/93‑1 (MQ=255)
                                  aaCTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGc                                                  >  2:4968268/1‑93 (MQ=255)
                                  aaCTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGc                                                  >  2:663762/1‑93 (MQ=255)
                                   aCTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCt                                                 >  2:3122054/1‑93 (MQ=255)
                                      gtgAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCg                                              <  1:6173201/93‑1 (MQ=255)
                                       tgAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGa                                             <  1:5841327/93‑1 (MQ=255)
                                       tgAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGa                                             >  1:2133205/1‑93 (MQ=255)
                                          gTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGt                                          >  1:2180438/1‑93 (MQ=255)
                                          gTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGt                                          <  2:1072976/93‑1 (MQ=255)
                                          gTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGt                                          >  1:1817499/1‑93 (MQ=255)
                                            aCCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTc                                        >  1:445612/1‑93 (MQ=255)
                                            aCCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTc                                        <  2:1778529/93‑1 (MQ=255)
                                             cccTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTcc                                       >  2:512730/1‑93 (MQ=255)
                                              ccTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTccc                                      <  1:5166035/93‑1 (MQ=255)
                                              ccTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTccc                                      >  2:1226281/1‑93 (MQ=255)
                                              ccTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTccc                                      >  2:1250540/1‑93 (MQ=255)
                                               cTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTcccc                                     >  1:1567440/1‑93 (MQ=255)
                                                tttCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCa                                    >  2:3218543/1‑93 (MQ=255)
                                                 ttCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCaa                                   >  1:2241928/1‑93 (MQ=255)
                                                  tCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAAc                                  >  2:650913/1‑93 (MQ=255)
                                                  tCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAAc                                  <  1:3789463/93‑1 (MQ=255)
                                                  tCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAAc                                  <  2:5613651/93‑1 (MQ=255)
                                                     cTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGc                               <  1:3030355/93‑1 (MQ=255)
                                                     cTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGc                               <  2:4055600/93‑1 (MQ=255)
                                                     cTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGc                               >  2:5417600/1‑93 (MQ=255)
                                                     cTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGACGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGc                               >  2:6163428/1‑93 (MQ=255)
                                                      tttACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCt                              >  1:1681818/1‑93 (MQ=255)
                                                      tttACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCt                              >  1:3502986/1‑93 (MQ=255)
                                                       ttACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCtt                             >  2:6040058/1‑93 (MQ=255)
                                                       ttACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCtt                             <  1:3428642/93‑1 (MQ=255)
                                                        tACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCttt                            >  2:3671062/1‑93 (MQ=255)
                                                         aCCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTc                           <  1:2524027/93‑1 (MQ=255)
                                                             ggCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTctgct                       <  1:2794/93‑1 (MQ=255)
                                                               cgcATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCtgctgc                     >  2:4710331/1‑93 (MQ=255)
                                                                gcATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCa                    >  1:933756/1‑93 (MQ=255)
                                                                gcATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCa                    <  2:3935525/93‑1 (MQ=255)
                                                                gcATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGGTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCCACCGCTTTCtgctgcg                    >  1:1200036/1‑92 (MQ=255)
                                                                   ttGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTTCAGcc                 <  1:4060482/93‑1 (MQ=255)
                                                                   ttGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGcc                 <  1:1060325/93‑1 (MQ=255)
                                                                    tGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCa                <  1:984626/93‑1 (MQ=255)
                                                                     ggTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCAt               <  2:6358659/93‑1 (MQ=255)
                                                                     ggTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCAt               <  1:1160946/93‑1 (MQ=255)
                                                                       tACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCAtcg             <  1:2650890/93‑1 (MQ=255)
                                                                        aCAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCAtcgt            >  2:5867579/1‑93 (MQ=255)
                                                                        aCAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGATTTCTGCTGCAGCCAtcgt            >  1:5226530/1‑93 (MQ=255)
                                                                         cAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCAtcgtc           >  2:4596525/1‑93 (MQ=255)
                                                                         cAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCAtcgtc           >  1:3352733/1‑93 (MQ=255)
                                                                          aaCGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCAtcgtcg          >  2:3047348/1‑93 (MQ=255)
                                                                           aCGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGc         <  1:6423156/93‑1 (MQ=255)
                                                                            cGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGcc        <  2:2802547/93‑1 (MQ=255)
                                                                            cGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGcc        <  2:1632691/93‑1 (MQ=255)
                                                                             gTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGcca       >  2:2564421/1‑93 (MQ=255)
                                                                             gTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGcca       >  1:4734153/1‑93 (MQ=255)
                                                                              tCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGccac      <  2:2116679/93‑1 (MQ=255)
                                                                               ccTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGccacc     >  2:1877149/1‑93 (MQ=255)
                                                                               ccTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGccacc     >  1:3606318/1‑93 (MQ=255)
                                                                               ccTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGccacc     <  1:3896316/93‑1 (MQ=255)
                                                                                cgttCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGccacca    <  1:1519828/91‑1 (MQ=255)
                                                                                cTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGccacca    <  2:6442942/93‑1 (MQ=255)
                                                                                cTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGccacca    <  1:4832276/93‑1 (MQ=255)
                                                                                 tttCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGCCACCAt   <  1:5653179/93‑1 (MQ=255)
                                                                                       ||                                                                                      
GACATAGGCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATCGATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGCCACCATA  >  NC_000913/3734177‑3734351

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGACATAGGCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATCGATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGCCACCATACT  >  NC_000913/3734176‑3734353
                                                                                        |                                                                                         
TGACATAGGCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTT                                                                                      >  SRR4302534.959497/1‑94 (MQ=60)
 GACATAGGCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTC                                                                                     <  SRR4302534.2094392/94‑1 (MQ=60)
  ACATAGGCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCA                                                                                    >  SRR4302534.2823086/1‑94 (MQ=60)
   CATAGGCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCAT                                                                                   <  SRR4302534.984706/94‑1 (MQ=60)
    ATAGGCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATG                                                                                  <  SRR4302534.6592338/94‑1 (MQ=60)
     TAGGCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGC                                                                                 >  SRR4302534.2221255/1‑94 (MQ=60)
      AGGCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCC                                                                                >  SRR4302534.1192466/1‑94 (MQ=60)
       GGCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCG                                                                               <  SRR4302534.2645062/94‑1 (MQ=60)
         CGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAA                                                                             >  SRR4302534.5115696/1‑94 (MQ=60)
         CGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAA                                                                             <  SRR4302534.4111562/94‑1 (MQ=60)
          GCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAAC                                                                            >  SRR4302534.1138040/1‑94 (MQ=60)
          GCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAAC                                                                            <  SRR4302534.536902/94‑1 (MQ=60)
          GCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAAC                                                                            <  SRR4302534.5706595/94‑1 (MQ=60)
           CTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACA                                                                           <  SRR4302534.2468076/94‑1 (MQ=60)
           CTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACA                                                                           >  SRR4302534.5764544/1‑94 (MQ=60)
           CTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACA                                                                           >  SRR4302534.3483235/1‑94 (MQ=60)
           CTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACA                                                                           <  SRR4302534.6413590/94‑1 (MQ=60)
           CTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACA                                                                           <  SRR4302534.5891784/94‑1 (MQ=60)
           CTCACCGATTCTTTGACAGAACGAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACA                                                                           <  SRR4302534.4996600/94‑1 (MQ=60)
            TCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTCGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAG                                                                          >  SRR4302534.3523421/1‑94 (MQ=60)
             CACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGG                                                                         <  SRR4302534.5446721/94‑1 (MQ=60)
             CACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGG                                                                         >  SRR4302534.1694272/1‑94 (MQ=60)
             CACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGG                                                                         <  SRR4302534.1668438/94‑1 (MQ=60)
             CACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGG                                                                         >  SRR4302534.4184184/1‑94 (MQ=60)
              ACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGT                                                                        >  SRR4302534.5090274/1‑94 (MQ=60)
              ACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGT                                                                        <  SRR4302534.6009026/94‑1 (MQ=60)
               CCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTT                                                                       <  SRR4302534.6429946/94‑1 (MQ=60)
               CCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTT                                                                       <  SRR4302534.4926202/94‑1 (MQ=60)
               CCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTT                                                                       <  SRR4302534.4265776/94‑1 (MQ=60)
               CCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATATCATGCCGAACAGGTT                                                                       >  SRR4302534.589027/1‑94 (MQ=60)
               CCGATTCTTTGACAGAACTAAACTGTCAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTT                                                                       <  SRR4302534.5343790/94‑1 (MQ=60)
                CGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTG                                                                      <  SRR4302534.6291319/94‑1 (MQ=60)
                CGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAAAAGGTTG                                                                      >  SRR4302534.1439407/1‑94 (MQ=60)
                 GATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGT                                                                     <  SRR4302534.502316/94‑1 (MQ=60)
                 GATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGT                                                                     >  SRR4302534.1711426/1‑94 (MQ=60)
                  ATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTT                                                                    >  SRR4302534.751070/1‑94 (MQ=60)
                  ATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTT                                                                    >  SRR4302534.4401277/1‑94 (MQ=60)
                  ATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTT                                                                    >  SRR4302534.4891925/1‑94 (MQ=60)
                  ATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTT                                                                    <  SRR4302534.5204129/94‑1 (MQ=60)
                  ATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTT                                                                    >  SRR4302534.6400316/1‑94 (MQ=60)
                  ATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTT                                                                    >  SRR4302534.1546117/1‑94 (MQ=60)
                   TTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTG                                                                   >  SRR4302534.1116224/1‑94 (MQ=60)
                    TCTTTGACAGAGCTATACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATTCATTTCATGCCGAACAGGTTGTTGT                                                                  <  SRR4302534.256684/94‑1 (MQ=60)
                    TCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGT                                                                  >  SRR4302534.6122654/1‑94 (MQ=60)
                     CTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTT                                                                 <  SRR4302534.236633/94‑1 (MQ=60)
                     CTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTT                                                                 >  SRR4302534.5400002/1‑94 (MQ=60)
                     CTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTT                                                                 >  SRR4302534.1897264/1‑94 (MQ=60)
                     CTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTT                                                                 <  SRR4302534.2085358/94‑1 (MQ=60)
                     CTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTAATGCCGAACAGGTTGTTGTT                                                                 >  SRR4302534.4397778/1‑94 (MQ=60)
                      TTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGCTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTG                                                                >  SRR4302534.3427162/1‑94 (MQ=60)
                      TTTGACAGAACTAAACTGTAAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTG                                                                >  SRR4302534.3172750/1‑94 (MQ=60)
                       TTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGT                                                               <  SRR4302534.4926424/94‑1 (MQ=60)
                        TGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTT                                                              >  SRR4302534.1151481/1‑94 (MQ=60)
                        TGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTT                                                              >  SRR4302534.664450/1‑94 (MQ=60)
                        TGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTT                                                              <  SRR4302534.2788868/94‑1 (MQ=60)
                        TGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTT                                                              <  SRR4302534.235273/94‑1 (MQ=60)
                         GACAGAACTAAACTGTGAGTGCCCTGTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTG                                                             <  SRR4302534.2013597/94‑1 (MQ=60)
                         GACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTG                                                             <  SRR4302534.1703833/94‑1 (MQ=60)
                         GACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTG                                                             >  SRR4302534.1910045/1‑94 (MQ=60)
                          ACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGC                                                            >  SRR4302534.1163218/1‑94 (MQ=60)
                          ACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGC                                                            >  SRR4302534.1784990/1‑94 (MQ=60)
                          ACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGC                                                            >  SRR4302534.5676884/1‑94 (MQ=60)
                           CAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCG                                                           <  SRR4302534.3225563/94‑1 (MQ=60)
                           CAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCG                                                           >  SRR4302534.6400265/1‑94 (MQ=60)
                           CAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCG                                                           <  SRR4302534.4148652/94‑1 (MQ=60)
                           CAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTAGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCG                                                           <  SRR4302534.2271323/94‑1 (MQ=60)
                            AGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGC                                                          >  SRR4302534.5657963/1‑94 (MQ=60)
                            AGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGC                                                          >  SRR4302534.4242302/1‑94 (MQ=60)
                            AGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGC                                                          >  SRR4302534.91722/1‑94 (MQ=60)
                            AGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGC                                                          >  SRR4302534.1534598/1‑94 (MQ=60)
                            AGAACTAAACTATGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGC                                                          >  SRR4302534.6018663/1‑94 (MQ=60)
                             GAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCG                                                         <  SRR4302534.5255405/94‑1 (MQ=60)
                              AACTAATCTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGC                                                        <  SRR4302534.1573945/94‑1 (MQ=60)
                              AACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGC                                                        >  SRR4302534.2130147/1‑94 (MQ=60)
                              AACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGC                                                        >  SRR4302534.4028823/1‑94 (MQ=60)
                               ACTAAACTGTGAGTACTCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGTATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCC                                                       >  SRR4302534.6250060/1‑94 (MQ=60)
                               ACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCC                                                       <  SRR4302534.2994473/94‑1 (MQ=60)
                                CTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCA                                                      >  SRR4302534.2612016/1‑94 (MQ=60)
                                CTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCA                                                      <  SRR4302534.6024060/94‑1 (MQ=60)
                                CTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCA                                                      >  SRR4302534.2603491/1‑94 (MQ=60)
                                 TAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAG                                                     <  SRR4302534.2921014/94‑1 (MQ=60)
                                 TAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAG                                                     <  SRR4302534.4535953/94‑1 (MQ=60)
                                 TAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAG                                                     >  SRR4302534.5062095/1‑94 (MQ=60)
                                  AAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGC                                                    >  SRR4302534.5207417/1‑94 (MQ=60)
                                  AAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGC                                                    <  SRR4302534.239541/94‑1 (MQ=60)
                                   AACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCT                                                   >  SRR4302534.4575860/1‑94 (MQ=60)
                                   AACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCT                                                   >  SRR4302534.2229856/1‑94 (MQ=60)
                                   AACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCT                                                   <  SRR4302534.4267909/94‑1 (MQ=60)
                                    ACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTT                                                  <  SRR4302534.3222005/94‑1 (MQ=60)
                                      TGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCG                                                <  SRR4302534.2688610/94‑1 (MQ=60)
                                      TGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCG                                                <  SRR4302534.1651172/94‑1 (MQ=60)
                                       GTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGA                                               >  SRR4302534.2189409/1‑94 (MQ=60)
                                       GTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGA                                               <  SRR4302534.6341143/94‑1 (MQ=60)
                                       GTGAGTACCCTTCCACTGTCCCTGGCGCATTCGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGA                                               <  SRR4302534.4583918/94‑1 (MQ=60)
                                        TGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGAC                                              <  SRR4302534.5999890/94‑1 (MQ=60)
                                        TGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGAC                                              <  SRR4302534.3957179/94‑1 (MQ=60)
                                         GAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACG                                             >  SRR4302534.5398695/1‑94 (MQ=60)
                                         GAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACG                                             >  SRR4302534.1748663/1‑94 (MQ=60)
                                         GAGTACCCTATCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACG                                             >  SRR4302534.6471022/1‑94 (MQ=60)
                                          AGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGT                                            >  SRR4302534.2238067/1‑94 (MQ=60)
                                          AGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGT                                            >  SRR4302534.2431483/1‑94 (MQ=60)
                                          AGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGT                                            <  SRR4302534.1257658/94‑1 (MQ=60)
                                          AGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGT                                            >  SRR4302534.1864723/1‑94 (MQ=60)
                                           GTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTT                                           <  SRR4302534.1971987/94‑1 (MQ=60)
                                            TACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTC                                          >  SRR4302534.456919/1‑94 (MQ=60)
                                            TACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTC                                          >  SRR4302534.6633935/1‑94 (MQ=60)
                                             ACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCC                                         >  SRR4302534.2192234/1‑94 (MQ=60)
                                             ACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCC                                         >  SRR4302534.5557825/1‑94 (MQ=60)
                                             ACCCTTTCACTTTACCTGGAGCATTGGTAAAACGTACTTTCATGCATTTCATGCCGAACAGGTTGTTGCTGTTGAGCGCCAGCGTCGACGTTCC                                         >  SRR4302534.6033029/1‑94 (MQ=60)
                                              CCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCC                                        <  SRR4302534.6032146/94‑1 (MQ=60)
                                               CCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCC                                       >  SRR4302534.4955342/1‑94 (MQ=60)
                                               CCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCC                                       >  SRR4302534.6617274/1‑94 (MQ=60)
                                               CCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCC                                       >  SRR4302534.1608496/1‑94 (MQ=60)
                                               CCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCC                                       <  SRR4302534.5305288/94‑1 (MQ=60)
                                                CTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCA                                      <  SRR4302534.3456969/94‑1 (MQ=60)
                                                 TTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGTGCCAGCCTCGACGgtaccccc                                     >  SRR4302534.5681556/1‑86 (MQ=60)
                                                 TTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAA                                     >  SRR4302534.2301338/1‑94 (MQ=60)
                                                   TCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACC                                   <  SRR4302534.3891199/94‑1 (MQ=60)
                                                    CACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCG                                  >  SRR4302534.445683/1‑94 (MQ=60)
                                                    CACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCG                                  >  SRR4302534.1225796/1‑94 (MQ=60)
                                                    CACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCG                                  >  SRR4302534.1154257/1‑94 (MQ=60)
                                                    CACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCG                                  >  SRR4302534.2271974/1‑94 (MQ=60)
                                                     ACTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGC                                 <  SRR4302534.1596951/94‑1 (MQ=60)
                                                      CTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCT                                <  SRR4302534.3729450/94‑1 (MQ=60)
                                                      CTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCT                                >  SRR4302534.3597216/1‑94 (MQ=60)
                                                      CTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCT                                <  SRR4302534.3729451/94‑1 (MQ=60)
                                                      CTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCT                                >  SRR4302534.1725562/1‑94 (MQ=60)
                                                      CTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCT                                >  SRR4302534.2021795/1‑94 (MQ=60)
                                                      CTTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCT                                <  SRR4302534.3109811/94‑1 (MQ=60)
                                                       TTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTT                               <  SRR4302534.5521791/94‑1 (MQ=60)
                                                       TTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTT                               >  SRR4302534.6308368/1‑94 (MQ=60)
                                                       TTTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTT                               <  SRR4302534.2409376/94‑1 (MQ=60)
                                                        TTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTT                              <  SRR4302534.43125/94‑1 (MQ=60)
                                                        TTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTT                              <  SRR4302534.3179449/94‑1 (MQ=60)
                                                        TTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTT                              <  SRR4302534.3520756/94‑1 (MQ=60)
                                                        TTACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTT                              <  SRR4302534.576231/94‑1 (MQ=60)
                                                          ACCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCT                            <  SRR4302534.2591009/94‑1 (MQ=60)
                                                           CCTGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTG                           <  SRR4302534.3537536/94‑1 (MQ=60)
                                                           CCTGGCGCATTAGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTG                           <  SRR4302534.4542931/94‑1 (MQ=60)
                                                             TGGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCT                         >  SRR4302534.4870344/1‑94 (MQ=60)
                                                              GGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTG                        <  SRR4302534.2880/94‑1 (MQ=60)
                                                              GGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTG                        >  SRR4302534.2073473/1‑94 (MQ=60)
                                                              GGCGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTG                        <  SRR4302534.2373961/94‑1 (MQ=60)
                                                                CGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCA                      >  SRR4302534.957511/1‑94 (MQ=60)
                                                                CGCATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGGTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCCACCGCTTTCTGCTGCG                      >  SRR4302534.1231612/1‑94 (MQ=60)
                                                                  CATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCTCCAGCGTCGACGGTCCCCAACAGCTTTCTGTTGCACC                    >  SRR4302534.3598700/1‑94 (MQ=60)
                                                                   ATTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCC                   >  SRR4302534.5099630/1‑94 (MQ=60)
                                                                    TTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTTCAGCCA                  <  SRR4302534.4169398/94‑1 (MQ=60)
                                                                    TTGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCA                  <  SRR4302534.1087810/94‑1 (MQ=60)
                                                                     TGGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCAT                 <  SRR4302534.1009778/94‑1 (MQ=60)
                                                                      GGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATC                <  SRR4302534.1191418/94‑1 (MQ=60)
                                                                      GGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATC                >  SRR4302534.3682230/1‑94 (MQ=60)
                                                                      GGTACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATC                <  SRR4302534.3939533/94‑1 (MQ=60)
                                                                        TACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGT              <  SRR4302534.2720999/94‑1 (MQ=60)
                                                                        TACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGATTTCTGCTGCAGCCATCGT              >  SRR4302534.5367614/1‑94 (MQ=60)
                                                                        TACAACGTCCTTTCATGCATTTCATGCCGAACAGGCTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGT              >  SRR4302534.1237138/1‑94 (MQ=60)
                                                                         ACAACGTCCTTTCATTCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTC             <  SRR4302534.3596928/94‑1 (MQ=60)
                                                                         ACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTC             >  SRR4302534.3442526/1‑94 (MQ=60)
                                                                         ACAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTC             <  SRR4302534.2519209/94‑1 (MQ=60)
                                                                          CAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCG            >  SRR4302534.5765691/1‑94 (MQ=60)
                                                                          CAACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCG            <  SRR4302534.2673173/94‑1 (MQ=60)
                                                                            ACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGCC          <  SRR4302534.6596387/94‑1 (MQ=60)
                                                                            ACGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGCC          >  SRR4302534.154929/1‑94 (MQ=60)
                                                                             CGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGCCA         >  SRR4302534.4862700/1‑94 (MQ=60)
                                                                             CGTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGCCA         <  SRR4302534.1012603/94‑1 (MQ=60)
                                                                              GTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGCCAC        <  SRR4302534.2335184/94‑1 (MQ=60)
                                                                              GTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGCCAC        >  SRR4302534.10122/1‑94 (MQ=60)
                                                                              GTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGCCAC        >  SRR4302534.443600/1‑94 (MQ=60)
                                                                              GTCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGCCAC        >  SRR4302534.4628960/1‑94 (MQ=60)
                                                                               TCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGCCACC       <  SRR4302534.3375066/94‑1 (MQ=60)
                                                                               TCCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGCCACC       >  SRR4302534.3703293/1‑94 (MQ=60)
                                                                                CCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGCCACCA      >  SRR4302534.2604872/1‑94 (MQ=60)
                                                                                CCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGCCACCA      <  SRR4302534.4000878/94‑1 (MQ=60)
                                                                                CCTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGCCACCA      <  SRR4302534.4906403/94‑1 (MQ=60)
                                                                                 CTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGCCACCAT     <  SRR4302534.5363406/94‑1 (MQ=60)
                                                                                 CTTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGCCACCAT     <  SRR4302534.4963376/94‑1 (MQ=60)
                                                                                  TTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGGGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGCCACCATA    >  SRR4302534.41602/1‑94 (MQ=60)
                                                                                  TTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGCCACCATA    <  SRR4302534.5806191/94‑1 (MQ=60)
                                                                                  TTTCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGCCACCATA    >  SRR4302534.5797806/1‑94 (MQ=60)
                                                                                   TTCATGCATTTCATGCCGAACAGCTGGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGCCACCATAC   <  SRR4302534.1665736/94‑1 (MQ=60)
                                                                                    TCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGCCACCATACT  >  SRR4302534.6377224/1‑94 (MQ=60)
                                                                                    TCATGCATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGCCACCATACT  <  SRR4302534.28432/94‑1 (MQ=60)
                                                                                        |                                                                                         
TGACATAGGCGCTCACCGATTCTTTGACAGAACTAAACTGTGAGTACCCTTTCACTTTACCTGGCGCATTGGTACAACGTCCTTTCATCGATTTCATGCCGAACAGGTTGTTGTTGTTGCGCGCCAGCTTCGACGTTCCCCAACCGCTTTCTGCTGCAGCCATCGTCGCCACCATACT  >  NC_000913/3734176‑3734353

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑