Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	884	257.2	12958910	96.9%	12557183	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,737,956	2 bp→CG	coding (623‑624/1254 nt)	avtA →	alanine‑alpha‑ketoisovalerate transaminase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,737,956	0	G	C	100.0%	803.0 / NA	230	G208A (GGC→GCG)	avtA	alanine‑alpha‑ketoisovalerate transaminase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base C (105/125); total (105/125)
*	NC_000913	3,737,957	0	C	G	100.0%	801.4 / NA	229	G208A (GGC→GCG)	avtA	alanine‑alpha‑ketoisovalerate transaminase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base G (104/125); total (104/125)

Only 100 of 235 total aligned reads displayed.

AAACCGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGGCAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTATG  >  NC_000913/3737869‑3738043

||

  aCCGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCaa                                                                                  <  2:1188516/93‑1 (MQ=255)

   ccGTGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAac                                                                                 <  1:1577508/93‑1 (MQ=255)

   ccGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTCCTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAac                                                                                 <  2:3447586/93‑1 (MQ=255)

   ccGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCCGGCGAATCAac                                                                                 >  2:108248/1‑93 (MQ=255)

    cGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAaca                                                                                >  2:4166863/1‑93 (MQ=255)

    cGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAaca                                                                                <  2:188553/93‑1 (MQ=255)

     tggATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAacac                                                                               >  2:6289374/2‑93 (MQ=255)

     gggATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAacac                                                                               >  1:553011/1‑93 (MQ=255)

     gggATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAacac                                                                               >  1:2281131/1‑93 (MQ=255)

       gatgatTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACgg                                                                             <  2:5828627/93‑1 (MQ=255)

        atgatTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGc                                                                            <  2:2608463/93‑1 (MQ=255)

         tgatTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCa                                                                           <  1:6011050/93‑1 (MQ=255)

            tttGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTc                                                                        <  1:932229/93‑1 (MQ=255)

            tttGCGCCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTc                                                                        <  1:5463008/93‑1 (MQ=255)

              tGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCg                                                                      >  2:2940656/1‑93 (MQ=255)

              tGCGACTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACAAGGCATTGCg                                                                      >  2:1455440/1‑93 (MQ=255)

               gCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGc                                                                     <  2:1248019/93‑1 (MQ=255)

               gCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGc                                                                     <  1:1653825/93‑1 (MQ=255)

                   ctcCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGt                                                                 >  2:4196280/1‑93 (MQ=255)

                   ctcCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGt                                                                 <  2:2759420/93‑1 (MQ=255)

                      ccGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGtgat                                                              <  1:3104710/93‑1 (MQ=255)

                      ccGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGtgat                                                              <  1:5812827/93‑1 (MQ=255)

                       cGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGtgatt                                                             >  1:6378460/1‑93 (MQ=255)

                        ggCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGtgattg                                                            >  2:2154640/1‑93 (MQ=255)

                         gCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGtgattga                                                           >  2:5382045/1‑93 (MQ=255)

                          ccGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGtgattgat                                                          >  2:83370/1‑93 (MQ=255)

                          ccGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGtgattgat                                                          >  2:4078846/1‑93 (MQ=255)

                          ccGACGAATCCAACAGGCAATGGGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGtgattgat                                                          >  1:6475445/1‑93 (MQ=255)

                           cgacgaATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATa                                                         <  2:5699566/93‑1 (MQ=255)

                           cgacgaATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATa                                                         >  2:6157026/1‑93 (MQ=255)

                            gacgaATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATaa                                                        >  2:3985452/1‑93 (MQ=255)

                            gacgaATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATaa                                                        >  1:3976197/1‑93 (MQ=255)

                            gacgaATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATaa                                                        >  2:4758357/1‑93 (MQ=255)

                            gacgaATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATaa                                                        >  2:5084149/1‑93 (MQ=255)

                             acgaATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACCCGGCCTTCCGCTGGTGATTGATAAc                                                       >  1:1540076/1‑93 (MQ=255)

                             acgaATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAAc                                                       <  1:4260166/93‑1 (MQ=255)

                               gaATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGTCATTCCGCTGGTGATTGATAACGc                                                     <  1:2034434/93‑1 (MQ=255)

                               gaATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGc                                                     >  1:2959211/1‑93 (MQ=255)

                               gaATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGc                                                     <  1:2473850/93‑1 (MQ=255)

                                 aTCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCtt                                                   >  1:4478517/1‑93 (MQ=255)

                                  tCCAACAGGCAATGGGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTa                                                  <  1:5576412/93‑1 (MQ=255)

                                  accAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTa                                                  <  1:3674098/92‑1 (MQ=255)

                                   ccAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTAt                                                 <  1:5130794/93‑1 (MQ=255)

                                    cAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGTTTATg                                                >  1:3358754/1‑93 (MQ=255)

                                    cAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATg                                                <  1:352577/93‑1 (MQ=255)

                                     aaCAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATgg                                               <  1:1696592/93‑1 (MQ=255)

                                     aaCAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATgg                                               <  1:6169509/93‑1 (MQ=255)

                                      aCAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATggg                                              >  1:2492938/1‑92 (MQ=255)

                                      aCAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGc                                              >  2:1757049/1‑93 (MQ=255)

                                      aCAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGc                                              <  1:1169014/93‑1 (MQ=255)

                                        aGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGt                                            >  2:5918989/1‑93 (MQ=255)

                                         ggCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTc                                           >  1:4372383/1‑93 (MQ=255)

                                           cAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTccc                                         <  2:2470366/93‑1 (MQ=255)

                                           cAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTccc                                         <  2:2255910/93‑1 (MQ=255)

                                            aaTGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCg                                        >  1:4583693/1‑93 (MQ=255)

                                              tgtgATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGtt                                      <  1:3143256/93‑1 (MQ=255)

                                              tgtgATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGtt                                      <  2:3388883/93‑1 (MQ=255)

                                              tgtgATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGtt                                      <  2:306408/93‑1 (MQ=255)

                                               gtgATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTc                                     <  1:2703798/93‑1 (MQ=255)

                                                tgATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTcc                                    <  1:2592121/93‑1 (MQ=255)

                                                   ttACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCgg                                 >  2:4540825/1‑93 (MQ=255)

                                                    tACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCggg                                <  1:6251579/93‑1 (MQ=255)

                                                    tACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCggg                                <  1:4495275/93‑1 (MQ=255)

                                                     aCTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGt                               <  1:124100/93‑1 (MQ=255)

                                                     aCTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGt                               >  2:3329181/1‑93 (MQ=255)

                                                      cTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTa                              <  1:4839424/93‑1 (MQ=255)

                                                       tGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTat                             >  1:1329494/1‑93 (MQ=255)

                                                          cGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTatcat                          >  1:5713784/1‑93 (MQ=255)

                                                          cGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTatcat                          >  1:4910475/1‑93 (MQ=255)

                                                           gAAGAGTTGCTGAAGCTTGACTCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATTGCGTCCCGTTTCCGGGTatcatc                         >  1:4088917/1‑93 (MQ=255)

                                                           gAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAGCGCTTATGGCGTCCCGTTCCCGGGTatcatc                         <  2:3745817/93‑1 (MQ=255)

                                                           gAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTctcatc                         >  2:1953568/1‑93 (MQ=255)

                                                           gAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTatcatc                         <  1:4032888/93‑1 (MQ=255)

                                                            aaGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCt                        <  1:2135097/93‑1 (MQ=255)

                                                            aaGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGCTCCCGGGCATCATCt                        >  1:1198234/1‑93 (MQ=255)

                                                                gTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAg                    <  1:287006/93‑1 (MQ=255)

                                                                 ttGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGt                   >  1:5292100/1‑93 (MQ=255)

                                                                  tGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTg                  <  1:1454733/93‑1 (MQ=255)

                                                                   gCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAGCGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGa                 <  2:5490006/93‑1 (MQ=255)

                                                                   gCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGa                 >  1:967822/1‑93 (MQ=255)

                                                                   gCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGa                 <  2:5502838/93‑1 (MQ=255)

                                                                    cTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATTATCTTCAGTGaa                <  2:3464939/93‑1 (MQ=255)

                                                                    cTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGaa                >  2:521412/1‑93 (MQ=255)

                                                                     tGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAg               <  2:5224936/93‑1 (MQ=255)

                                                                      gAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAgc              <  2:3199528/93‑1 (MQ=255)

                                                                      gAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAgc              <  2:1698825/93‑1 (MQ=255)

                                                                       aaGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAgcg             <  2:5162566/93‑1 (MQ=255)

                                                                       aaGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAgcg             <  1:5931543/93‑1 (MQ=255)

                                                                       aaGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAgcg             <  1:3460694/93‑1 (MQ=255)

                                                                        aGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAgcgc            >  1:5875821/1‑93 (MQ=255)

                                                                         gCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAgcgcg           >  2:2648516/1‑93 (MQ=255)

                                                                         gCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAgcgcg           >  1:827909/1‑93 (MQ=255)

                                                                           ttGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGcc         >  1:3491577/1‑93 (MQ=255)

                                                                           ttGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGcc         <  2:2001796/93‑1 (MQ=255)

                                                                            tGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGccc        <  2:3008578/93‑1 (MQ=255)

                                                                            tGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGccc        <  2:2114068/93‑1 (MQ=255)

                                                                            tGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGccc        >  1:1024107/1‑93 (MQ=255)

                                                                                 cgcTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTAt   <  1:3944471/93‑1 (MQ=255)

                                                                                  gcTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTATg  <  1:493421/93‑1 (MQ=255)

                                                                                  gcTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTATg  <  1:5184666/93‑1 (MQ=255)

||

AAACCGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGGCAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTATG  >  NC_000913/3737869‑3738043

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GCGAAGAAACCGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGGCAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTATGGA  >  NC_000913/3737863‑3738045

GCGAAGAAACCGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGC                                                                                           <  SRR4302534.910920/94‑1 (MQ=60)

     GAAACCGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATC                                                                                      <  SRR4302534.1874249/94‑1 (MQ=60)

      AAACCGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCA                                                                                     >  SRR4302534.1721473/1‑94 (MQ=60)

         CCGTGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACA                                                                                  <  SRR4302534.1618781/94‑1 (MQ=60)

         CCGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGATTGACGCGCTGGCGAATCAACA                                                                                  >  SRR4302534.1255389/1‑94 (MQ=60)

          CGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACAC                                                                                 <  SRR4302534.2259376/94‑1 (MQ=60)

          CGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACAC                                                                                 >  SRR4302534.566643/1‑94 (MQ=60)

          CGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACAC                                                                                 >  SRR4302534.2341659/1‑94 (MQ=60)

          CGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACAC                                                                                 <  SRR4302534.4862808/94‑1 (MQ=60)

           GTGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACG                                                                                <  SRR4302534.538677/94‑1 (MQ=60)

           GGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACG                                                                                >  SRR4302534.6153061/1‑94 (MQ=60)

           GGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACG                                                                                >  SRR4302534.3629726/1‑94 (MQ=60)

           GGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACG                                                                                <  SRR4302534.1558684/94‑1 (MQ=60)

            GGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGG                                                                               <  SRR4302534.4074519/94‑1 (MQ=60)

            GGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGG                                                                               >  SRR4302534.1268976/1‑94 (MQ=60)

             GATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGC                                                                              <  SRR4302534.5690025/94‑1 (MQ=60)

              ATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCA                                                                             <  SRR4302534.2347844/94‑1 (MQ=60)

               TGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCAT                                                                            <  SRR4302534.6174373/94‑1 (MQ=60)

               TGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCAT                                                                            >  SRR4302534.5677201/1‑94 (MQ=60)

                 ATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTC                                                                          >  SRR4302534.4601707/1‑94 (MQ=60)

                  TTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCC                                                                         <  SRR4302534.955946/94‑1 (MQ=60)

                  TTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCC                                                                         >  SRR4302534.269112/1‑94 (MQ=60)

                  TTTGCGCCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCC                                                                         <  SRR4302534.5610256/94‑1 (MQ=60)

                    TGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGC                                                                       >  SRR4302534.3567/1‑94 (MQ=60)

                     GCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCT                                                                      <  SRR4302534.1696924/94‑1 (MQ=60)

                      CGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTG                                                                     <  SRR4302534.361126/94‑1 (MQ=60)

                      CGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTG                                                                     >  SRR4302534.978081/1‑94 (MQ=60)

                          TCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGA                                                                 <  SRR4302534.1656801/94‑1 (MQ=60)

                           CCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGAT                                                                <  SRR4302534.6025928/94‑1 (MQ=60)

                           CCCGGCCGACGAATCCAACACGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGAT                                                                >  SRR4302534.6447853/1‑94 (MQ=60)

                            CCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATT                                                               >  SRR4302534.2101556/1‑94 (MQ=60)

                            CCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATT                                                               <  SRR4302534.5970596/94‑1 (MQ=60)

                            CCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATT                                                               <  SRR4302534.3186366/94‑1 (MQ=60)

                            CCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATT                                                               >  SRR4302534.6550744/1‑94 (MQ=60)

                             CGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTTACGCGCTGGCGAATCAACACGGCATTCCGATGGTGATTT                                                              <  SRR4302534.5298025/94‑1 (MQ=60)

                              GGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGA                                                             >  SRR4302534.1575961/1‑94 (MQ=60)

                               GCCGACGAATCCAACAGGCAATGGGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGAT                                                            >  SRR4302534.6649904/1‑94 (MQ=60)

                                 CGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAA                                                          >  SRR4302534.4082606/1‑94 (MQ=60)

                                 CGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAA                                                          >  SRR4302534.3392213/1‑94 (MQ=60)

                                  GACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACCCGGCCTTCCGCTGGTGATTGATAAC                                                         >  SRR4302534.1580451/1‑94 (MQ=60)

                                  GACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAAC                                                         >  SRR4302534.6629691/1‑94 (MQ=60)

                                   ACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACG                                                        <  SRR4302534.4375209/94‑1 (MQ=60)

                                   ACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACG                                                        <  SRR4302534.2311951/94‑1 (MQ=60)

                                    CGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGC                                                       >  SRR4302534.3036753/1‑94 (MQ=60)

                                     GAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGTCATTCCGCTGGTGATTGATAACGCT                                                      <  SRR4302534.2087632/94‑1 (MQ=60)

                                     GAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTTATAACGCT                                                      >  SRR4302534.1290204/1‑94 (MQ=60)

                                     GAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCT                                                      <  SRR4302534.4998564/94‑1 (MQ=60)

                                     GAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCT                                                      <  SRR4302534.2539585/94‑1 (MQ=60)

                                      AATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTT                                                     >  SRR4302534.5979531/1‑94 (MQ=60)

                                      AATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTT                                                     >  SRR4302534.4599679/1‑94 (MQ=60)

                                      AATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTT                                                     <  SRR4302534.4150367/94‑1 (MQ=60)

                                      AATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGAGATTGATAAAGCTT                                                     >  SRR4302534.3559034/1‑94 (MQ=60)

                                        TCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTAT                                                   <  SRR4302534.1208869/94‑1 (MQ=60)

                                        TCCAACAGGCAATGGGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTAT                                                   <  SRR4302534.5727030/94‑1 (MQ=60)

                                        ACCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTAT                                                   <  SRR4302534.3772863/94‑1 (MQ=60)

                                         CCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGTTTATG                                                  >  SRR4302534.3448716/1‑94 (MQ=60)

                                         CCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATG                                                  <  SRR4302534.5269316/94‑1 (MQ=60)

                                         CCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATG                                                  <  SRR4302534.5269315/94‑1 (MQ=60)

                                          CAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGG                                                 <  SRR4302534.361602/94‑1 (MQ=60)

                                          CAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGG                                                 <  SRR4302534.6193511/94‑1 (MQ=60)

                                           AACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGG                                                >  SRR4302534.2559153/1‑94 (MQ=60)

                                           AACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGC                                                >  SRR4302534.319812/1‑94 (MQ=60)

                                           AACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGC                                                <  SRR4302534.1740681/94‑1 (MQ=60)

                                           AACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGC                                                <  SRR4302534.6337315/94‑1 (MQ=60)

                                            ACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCG                                               <  SRR4302534.1199718/94‑1 (MQ=60)

                                              AGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTC                                             >  SRR4302534.4490214/1‑94 (MQ=60)

                                               GGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCC                                            <  SRR4302534.3191763/94‑1 (MQ=60)

                                                 CAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCG                                          >  SRR4302534.4708060/1‑94 (MQ=60)

                                                  AATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGT                                         >  SRR4302534.1720264/1‑94 (MQ=60)

                                                   ATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTT                                        >  SRR4302534.797055/1‑94 (MQ=60)

                                                    TGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTC                                       <  SRR4302534.4874447/94‑1 (MQ=60)

                                                    TGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTC                                       >  SRR4302534.4092148/1‑94 (MQ=60)

                                                    TGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTC                                       <  SRR4302534.973223/94‑1 (MQ=60)

                                                    TGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTC                                       <  SRR4302534.3225925/94‑1 (MQ=60)

                                                     GTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCC                                      <  SRR4302534.5905912/94‑1 (MQ=60)

                                                     GTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCC                                      <  SRR4302534.2775209/94‑1 (MQ=60)

                                                      TGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCC                                     <  SRR4302534.2660734/94‑1 (MQ=60)

                                                      TGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCC                                     >  SRR4302534.4520036/1‑94 (MQ=60)

                                                      TGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCC                                     <  SRR4302534.5511063/94‑1 (MQ=60)

                                                      TGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCC                                     <  SRR4302534.2540934/94‑1 (MQ=60)

                                                      TGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCC                                     <  SRR4302534.349079/94‑1 (MQ=60)

                                                       GATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCG                                    <  SRR4302534.5257973/94‑1 (MQ=60)

                                                         TTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGG                                  <  SRR4302534.5061513/94‑1 (MQ=60)

                                                         TTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGG                                  <  SRR4302534.2637382/94‑1 (MQ=60)

                                                         TTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGG                                  <  SRR4302534.4207715/94‑1 (MQ=60)

                                                         TTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGG                                  <  SRR4302534.6052427/94‑1 (MQ=60)

                                                          TACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGT                                 <  SRR4302534.1479923/94‑1 (MQ=60)

                                                          TACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGT                                 <  SRR4302534.2958044/94‑1 (MQ=60)

                                                          TACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGT                                 <  SRR4302534.6421075/94‑1 (MQ=60)

                                                          TACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGT                                 <  SRR4302534.4616936/94‑1 (MQ=60)

                                                           ACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTA                                >  SRR4302534.3943648/1‑94 (MQ=60)

                                                           ACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTA                                <  SRR4302534.127377/94‑1 (MQ=60)

                                                            CTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTAT                               >  SRR4302534.1364544/1‑94 (MQ=60)

                                                            CTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTAT                               <  SRR4302534.4970692/94‑1 (MQ=60)

                                                            CTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCCGGTAT                               >  SRR4302534.6289057/1‑94 (MQ=60)

                                                             TGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGCATC                              >  SRR4302534.4642047/1‑94 (MQ=60)

                                                              GACGAAGAGTTGCTGAAGCTTGCCGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGTTAACGCTTATGGCGTCCCGTTCCCGGGTATCT                             >  SRR4302534.6173071/1‑94 (MQ=60)

                                                              GACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCA                             <  SRR4302534.944319/94‑1 (MQ=60)

                                                              GACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCA                             <  SRR4302534.5682263/94‑1 (MQ=60)

                                                               ACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCAT                            >  SRR4302534.5868580/1‑94 (MQ=60)

                                                               ACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCAT                            >  SRR4302534.5043568/1‑94 (MQ=60)

                                                                CGAAGAGTTGCTGAAGCTTGACTCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATTGCGTCCCGTTTCCGGGTATCATC                           >  SRR4302534.4198744/1‑94 (MQ=60)

                                                                 GAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCT                          <  SRR4302534.4140996/94‑1 (MQ=60)

                                                                 GAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGCTCCCGGGCATCATCT                          >  SRR4302534.1229762/1‑94 (MQ=60)

                                                                  AAGAGTTGCTTAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTTATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTT                         <  SRR4302534.5848337/94‑1 (MQ=60)

                                                                  AAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTT                         <  SRR4302534.2191392/94‑1 (MQ=60)

                                                                   AGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTC                        >  SRR4302534.1107258/1‑94 (MQ=60)

                                                                    GAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTGTGGCGTCCCGTTCCCGGGTATCATCTTCA                       >  SRR4302534.3108902/1‑94 (MQ=60)

                                                                      GTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGT                     >  SRR4302534.5434925/1‑94 (MQ=60)

                                                                      GTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGT                     <  SRR4302534.294428/94‑1 (MQ=60)

                                                                       TTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTG                    <  SRR4302534.5408572/94‑1 (MQ=60)

                                                                        TGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGA                   >  SRR4302534.992476/1‑94 (MQ=60)

                                                                        TGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGA                   <  SRR4302534.1492995/94‑1 (MQ=60)

                                                                        TGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGA                   >  SRR4302534.2799705/1‑94 (MQ=60)

                                                                           TGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGC                <  SRR4302534.4438748/94‑1 (MQ=60)

                                                                            GAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCG               <  SRR4302534.4187270/94‑1 (MQ=60)

                                                                            GAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCG               >  SRR4302534.1586360/1‑94 (MQ=60)

                                                                            GAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCG               >  SRR4302534.4505799/1‑94 (MQ=60)

                                                                             AAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGC              <  SRR4302534.6092624/94‑1 (MQ=60)

                                                                             AAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGC              >  SRR4302534.6035343/1‑94 (MQ=60)

                                                                             AAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGC              <  SRR4302534.3553717/94‑1 (MQ=60)

                                                                              AGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCG             >  SRR4302534.848747/1‑94 (MQ=60)

                                                                                CTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCC           >  SRR4302534.3585423/1‑94 (MQ=60)

                                                                                CTTGACGCGCTGGCGAATCAACACGGCAATCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCTCGGGTATCATTCTCAGTGAAGCGCGCC           >  SRR4302534.1139618/1‑94 (MQ=60)

                                                                                 TTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCC          >  SRR4302534.1050480/1‑94 (MQ=60)

                                                                                  TGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCG         >  SRR4302534.2633204/1‑94 (MQ=60)

                                                                                  TGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCG         >  SRR4302534.2052059/1‑94 (MQ=60)

                                                                                   GACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGC        >  SRR4302534.2989074/1‑94 (MQ=60)

                                                                                      GCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTAT     >  SRR4302534.384469/1‑94 (MQ=60)

                                                                                       CGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCTTCTTCAGTGAAGCGCGCCCGCTATG    <  SRR4302534.6395931/94‑1 (MQ=60)

                                                                                       CGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTATG    <  SRR4302534.4050099/94‑1 (MQ=60)

                                                                                        GCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTATGG   <  SRR4302534.5324420/94‑1 (MQ=60)

                                                                                        GCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTATGG   >  SRR4302534.3288804/1‑94 (MQ=60)

                                                                                        GCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTATGG   <  SRR4302534.505729/94‑1 (MQ=60)

                                                                                        GCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTATGG   <  SRR4302534.6562518/94‑1 (MQ=60)

                                                                                        GCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTATGG   >  SRR4302534.31167/1‑94 (MQ=60)

                                                                                         CTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTATGGA  <  SRR4302534.2513078/94‑1 (MQ=60)

GCGAAGAAACCGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGGCAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTATGGA  >  NC_000913/3737863‑3738045

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 7 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`