Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	884	257.2	12958910	96.9%	12557183	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,768,546	G→A	A154V (GCG→GTG)	yibH ←	putative membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,768,546	0	G	A	99.6%	1051.8 / NA	277	A154V (GCG→GTG)	yibH	putative membrane protein
Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (121/154); total (121/156)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 298 total aligned reads displayed.

TCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGGGTGGTGTTGGCCTGCGCTTCAGTG  >  NC_000913/3768456‑3768635

tCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTGAACgccacc                                                                                         >  1:5602662/1‑93 (MQ=255)

tCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCgccacc                                                                                         <  1:3717340/93‑1 (MQ=255)

 ccGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCt                                                                                        <  2:4369262/93‑1 (MQ=255)

 ccGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCt                                                                                        >  2:5793652/1‑93 (MQ=255)

 ccGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCt                                                                                        <  1:6428593/93‑1 (MQ=255)

 ccGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCt                                                                                        <  1:2380704/93‑1 (MQ=255)

   gCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTgg                                                                                      >  1:5897062/1‑93 (MQ=255)

   gCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTgg                                                                                      >  1:724983/1‑93 (MQ=255)

    ccACCGAGCCTTTCCCCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGc                                                                                     <  1:4416431/93‑1 (MQ=255)

    ccACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGc                                                                                     <  2:3372930/93‑1 (MQ=255)

      aCCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTg                                                                                   >  1:2096115/1‑93 (MQ=255)

      aCCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTg                                                                                   >  1:795518/1‑93 (MQ=255)

        cGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGcc                                                                                 <  1:1686412/93‑1 (MQ=255)

        cGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGcc                                                                                 <  2:6308544/93‑1 (MQ=255)

        cGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGcc                                                                                 <  1:1542479/93‑1 (MQ=255)

         gAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCt                                                                                >  1:1404795/1‑93 (MQ=255)

           gCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCttt                                                                              >  2:1444324/1‑93 (MQ=255)

            ccTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTc                                                                             <  2:3809950/93‑1 (MQ=255)

            ccTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTc                                                                             <  2:3907111/93‑1 (MQ=255)

            ccTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTc                                                                             <  2:207361/93‑1 (MQ=255)

              tttCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCaa                                                                           <  1:4614559/93‑1 (MQ=255)

                  aCCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTaa                                                                       <  1:4751438/93‑1 (MQ=255)

                   ccAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAAc                                                                      >  2:4604924/1‑93 (MQ=255)

                   ccAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAAc                                                                      >  2:767150/1‑93 (MQ=255)

                   ccAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAAc                                                                      <  2:1097866/93‑1 (MQ=255)

                   ccAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAAc                                                                      >  2:5598486/1‑93 (MQ=255)

                    cAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACg                                                                     >  2:4156251/1‑93 (MQ=255)

                    cAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACg                                                                     >  2:2284807/1‑93 (MQ=255)

                     aGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGt                                                                    <  1:3444820/93‑1 (MQ=255)

                     aGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGt                                                                    >  2:464429/1‑93 (MQ=255)

                     aGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGt                                                                    <  1:5950389/93‑1 (MQ=255)

                     aGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGt                                                                    <  2:3068501/93‑1 (MQ=255)

                      gcgcATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGtt                                                                   <  1:5210203/93‑1 (MQ=255)

                      gcgcATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGtt                                                                   >  2:2754044/1‑93 (MQ=255)

                        gcATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGa                                                                 >  1:2189072/1‑93 (MQ=255)

                        gcATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGa                                                                 <  2:1068358/93‑1 (MQ=255)

                         cATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGAt                                                                <  2:5418880/93‑1 (MQ=255)

                         cATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGAt                                                                >  1:2599150/1‑93 (MQ=255)

                          aTCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATa                                                               <  1:130058/93‑1 (MQ=255)

                           tCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATaa                                                              <  1:4958802/93‑1 (MQ=255)

                           tCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATaa                                                              <  2:52078/93‑1 (MQ=255)

                               gcgcGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAAtttt                                                          <  2:864616/93‑1 (MQ=255)

                               gcgcGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAAtttt                                                          <  1:6010125/93‑1 (MQ=255)

                               gcgcGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAAtttt                                                          <  1:1916535/93‑1 (MQ=255)

                                cgcgAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAAttttt                                                         <  2:1603428/93‑1 (MQ=255)

                                 gcgAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTa                                                        <  1:6149826/93‑1 (MQ=255)

                                    aGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAAc                                                     <  2:2628627/93‑1 (MQ=255)

                                     ggAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACa                                                    <  2:1366448/93‑1 (MQ=255)

                                     ggAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACa                                                    >  2:3119047/1‑93 (MQ=255)

                                      gAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAg                                                   <  2:4342336/93‑1 (MQ=255)

                                       aaaTTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGa                                                  >  2:4587581/1‑93 (MQ=255)

                                        aaTTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGAc                                                 <  2:4058940/93‑1 (MQ=255)

                                        aaTTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGAc                                                 <  1:5452688/93‑1 (MQ=255)

                                          ttttGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACgg                                               >  1:3346513/1‑93 (MQ=255)

                                          ttttGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACgg                                               <  1:6071642/93‑1 (MQ=255)

                                           tttGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGt                                              <  2:5980044/93‑1 (MQ=255)

                                           tttGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGt                                              <  1:4810580/93‑1 (MQ=255)

                                            ttGCCGCGCATGGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACATGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTc                                             <  1:835576/93‑1 (MQ=255)

                                            ttGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTc                                             <  1:5240202/93‑1 (MQ=255)

                                            ttGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTc                                             <  2:2671543/93‑1 (MQ=255)

                                              gCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTcgc                                           >  2:1752076/1‑93 (MQ=255)

                                                 gcgcATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTTGCGCt                                        >  2:3260827/1‑93 (MQ=255)

                                                  cgcATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTc                                       >  2:2524811/1‑93 (MQ=255)

                                                  cgcAGCGTCGATGTCACGTTCCGAGACCGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTc                                       <  2:2702607/93‑1 (MQ=255)

                                                   gcATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTcc                                      >  2:1284232/1‑93 (MQ=255)

                                                    cATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCg                                     <  1:2802351/93‑1 (MQ=255)

                                                     aTCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGc                                    >  1:628587/1‑93 (MQ=255)

                                                      tcgtcgATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCt                                   >  1:1103837/1‑93 (MQ=255)

                                                      tcgtcgATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCt                                   <  1:2991112/93‑1 (MQ=255)

                                                        gtcgATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGa                                 >  2:3012529/1‑93 (MQ=255)

                                                          cgATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGaac                               >  2:4262999/1‑92 (MQ=255)

                                                           gATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAAc                              >  2:6192116/1‑93 (MQ=255)

                                                            aTGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAAcc                             >  2:151212/1‑93 (MQ=255)

                                                            aTGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAAcc                             <  2:655992/93‑1 (MQ=255)

                                                              gTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTg                           >  1:1609190/1‑93 (MQ=255)

                                                              gTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTg                           >  1:1973811/1‑93 (MQ=255)

                                                              gTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTg                           >  1:2890350/1‑93 (MQ=255)

                                                               tCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTgg                          >  1:3946540/1‑93 (MQ=255)

                                                                 aCGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTgtgt                        <  2:5082778/93‑1 (MQ=255)

                                                                 aCGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGggt                        <  2:56990/93‑1 (MQ=255)

                                                                 aCGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGggt                        >  2:3659523/1‑93 (MQ=255)

                                                                 aCGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGggt                        >  2:2430147/1‑93 (MQ=255)

                                                                   gTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGggtgg                      <  1:3534431/93‑1 (MQ=255)

                                                                      ccGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGGGTGgtgt                   <  1:4082603/93‑1 (MQ=255)

                                                                      ccGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGGGTGgtgt                   <  1:944527/93‑1 (MQ=255)

                                                                      ccGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGGGTGgtgt                   <  2:5544964/93‑1 (MQ=255)

                                                                        gagaACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGGGTGGTGTTg                 <  1:3122727/93‑1 (MQ=255)

                                                                        gagaACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGGGTGGTGTTg                 >  1:4277765/1‑93 (MQ=255)

                                                                         agaACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGGGTGGTGTTgg                >  1:1151663/1‑93 (MQ=255)

                                                                           aaCGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGGGTGGTGTTGGcc              <  1:5829218/93‑1 (MQ=255)

                                                                              ggATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGGGTGGTGTTGGCCTgc           <  1:5367024/93‑1 (MQ=255)

                                                                                aTTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGGGTGGTGTTGGCCTgcgc         >  2:1211955/1‑93 (MQ=255)

                                                                                  tCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGGGTGGTGTTGGCCTGCGCtt       <  1:2278029/93‑1 (MQ=255)

                                                                                   cACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGGGTGGTGTTGGCCTGCGCTTc      >  1:5545655/1‑93 (MQ=255)

                                                                                   cACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGGGTGGTGTTGGCCTGCGCTTc      >  2:6390237/1‑93 (MQ=255)

                                                                                   cACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGGGTGGTGTTGGCCTGCGCTTc      <  1:1372449/93‑1 (MQ=255)

                                                                                     ccgccaCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGGGTGGTGTTGGCCTGCGCTTCAg    <  1:4141108/93‑1 (MQ=255)

                                                                                     ccgccaCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGGGTGGTGTTGGCCTGCGCTTCAg    >  1:3229391/1‑93 (MQ=255)

                                                                                     ccgccaCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGGGTGGTGTTGGCCTGCGCTTCAg    <  1:2724662/93‑1 (MQ=255)

                                                                                       gccaccTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGGGTGGTGTTGGCCTGCGCTTCAGTg  <  2:946277/93‑1 (MQ=255)

TCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGGGTGGTGTTGGCCTGCGCTTCAGTG  >  NC_000913/3768456‑3768635

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGGGTGGTGTTGGCCTGCGCTTCAGTGAGC  >  NC_000913/3768456‑3768638

TCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCT                                                                                           <  SRR4302534.3817278/94‑1 (MQ=60)

TCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCT                                                                                           >  SRR4302534.6133006/1‑94 (MQ=60)

TCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCT                                                                                           <  SRR4302534.535678/94‑1 (MQ=60)

 CCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTG                                                                                          <  SRR4302534.2894044/94‑1 (MQ=60)

 CCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTG                                                                                          <  SRR4302534.2443957/94‑1 (MQ=60)

 CCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTG                                                                                          <  SRR4302534.6601937/94‑1 (MQ=60)

 CCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTG                                                                                          <  SRR4302534.1610128/94‑1 (MQ=60)

  CGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGG                                                                                         >  SRR4302534.6057178/1‑94 (MQ=60)

  CGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGG                                                                                         >  SRR4302534.742882/1‑94 (MQ=60)

  CGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGG                                                                                         <  SRR4302534.892280/94‑1 (MQ=60)

   GCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGC                                                                                        >  SRR4302534.4470081/1‑94 (MQ=60)

    CCACCGAGCCTTTCCCCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCT                                                                                       <  SRR4302534.4535660/94‑1 (MQ=60)

    CCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCT                                                                                       <  SRR4302534.5792880/94‑1 (MQ=60)

    CCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCT                                                                                       <  SRR4302534.6011171/94‑1 (MQ=60)

    CCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCT                                                                                       >  SRR4302534.539074/1‑94 (MQ=60)

    CCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCT                                                                                       <  SRR4302534.244013/94‑1 (MQ=60)

     CACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTG                                                                                      >  SRR4302534.2151108/1‑94 (MQ=60)

     CACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTG                                                                                      <  SRR4302534.5183368/94‑1 (MQ=60)

     CACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTG                                                                                      >  SRR4302534.815477/1‑94 (MQ=60)

        CGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCT                                                                                   >  SRR4302534.5347283/1‑94 (MQ=60)

        CGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCT                                                                                   <  SRR4302534.1730269/94‑1 (MQ=60)

        CGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCT                                                                                   >  SRR4302534.1441739/1‑94 (MQ=60)

        CGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCT                                                                                   <  SRR4302534.1582906/94‑1 (MQ=60)

         GAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTT                                                                                  <  SRR4302534.78357/94‑1 (MQ=60)

          AGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTT                                                                                 <  SRR4302534.4713906/94‑1 (MQ=60)

          AGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTT                                                                                 >  SRR4302534.2286785/1‑94 (MQ=60)

            CCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCA                                                                               <  SRR4302534.6622252/94‑1 (MQ=60)

             CTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAA                                                                              >  SRR4302534.1375871/1‑94 (MQ=60)

              TTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAG                                                                             <  SRR4302534.6523601/94‑1 (MQ=60)

              TTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAG                                                                             <  SRR4302534.4739783/94‑1 (MQ=60)

              TTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAG                                                                             <  SRR4302534.5376366/94‑1 (MQ=60)

                 CACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAA                                                                          >  SRR4302534.1526660/1‑94 (MQ=60)

                 CACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAA                                                                          >  SRR4302534.3183927/1‑94 (MQ=60)

                 CACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAA                                                                          >  SRR4302534.5683820/1‑94 (MQ=60)

                  ACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAAC                                                                         <  SRR4302534.4880419/94‑1 (MQ=60)

                  ACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAAC                                                                         <  SRR4302534.3252518/94‑1 (MQ=60)

                   CCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACG                                                                        <  SRR4302534.3591068/94‑1 (MQ=60)

                   CCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACG                                                                        <  SRR4302534.4974979/94‑1 (MQ=60)

                   CCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACG                                                                        <  SRR4302534.3759622/94‑1 (MQ=60)

                    CAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGT                                                                       <  SRR4302534.3025343/94‑1 (MQ=60)

                     AGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTT                                                                      <  SRR4302534.6111960/94‑1 (MQ=60)

                     AGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTT                                                                      <  SRR4302534.3537398/94‑1 (MQ=60)

                      GCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTG                                                                     <  SRR4302534.5350816/94‑1 (MQ=60)

                      GCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTG                                                                     <  SRR4302534.6620843/94‑1 (MQ=60)

                      GCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTG                                                                     <  SRR4302534.4293945/94‑1 (MQ=60)

                       CGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGA                                                                    >  SRR4302534.2246954/1‑94 (MQ=60)

                       CGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGA                                                                    >  SRR4302534.6254717/1‑94 (MQ=60)

                       CGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGA                                                                    >  SRR4302534.1423849/1‑94 (MQ=60)

                        GCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGAT                                                                   >  SRR4302534.2667930/1‑94 (MQ=60)

                         CATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATA                                                                  >  SRR4302534.3980712/1‑94 (MQ=60)

                          ATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAA                                                                 <  SRR4302534.133498/94‑1 (MQ=60)

                           TCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGACCGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAAT                                                                <  SRR4302534.5523702/94‑1 (MQ=60)

                           TCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAAT                                                                <  SRR4302534.5093086/94‑1 (MQ=60)

                            CCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATT                                                               <  SRR4302534.1712354/94‑1 (MQ=60)

                            CCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATT                                                               <  SRR4302534.825723/94‑1 (MQ=60)

                             CTGCGCGCGGAAATTTTGGCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCGCCGCCCCCTGGCTGCCTCTCAAGTAACGTTGATAATTT                                                              <  SRR4302534.6497431/94‑1 (MQ=60)

                             CTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTT                                                              <  SRR4302534.2849922/94‑1 (MQ=60)

                              TGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTT                                                             <  SRR4302534.2775069/94‑1 (MQ=60)

                              TGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTT                                                             <  SRR4302534.351094/94‑1 (MQ=60)

                               GCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTT                                                            <  SRR4302534.6173423/94‑1 (MQ=60)

                               GCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTT                                                            <  SRR4302534.1966586/94‑1 (MQ=60)

                               GCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTT                                                            <  SRR4302534.3491256/94‑1 (MQ=60)

                                 GCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAA                                                          <  SRR4302534.6317116/94‑1 (MQ=60)

                                  CGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAA                                                         >  SRR4302534.1416701/1‑94 (MQ=60)

                                   GAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTCCAAGTAACGTTGATAATTTTTAAAC                                                        >  SRR4302534.5994955/1‑94 (MQ=60)

                                     GGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAG                                                      <  SRR4302534.2373748/94‑1 (MQ=60)

                                       AAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGAC                                                    <  SRR4302534.2474904/94‑1 (MQ=60)

                                        AATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACG                                                   <  SRR4302534.5599613/94‑1 (MQ=60)

                                         ATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGG                                                  >  SRR4302534.3436096/1‑94 (MQ=60)

                                         ATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGG                                                  >  SRR4302534.2028307/1‑94 (MQ=60)

                                          TTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGT                                                 <  SRR4302534.2907269/94‑1 (MQ=60)

                                          TTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGT                                                 <  SRR4302534.5880407/94‑1 (MQ=60)

                                          TTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGT                                                 <  SRR4302534.6236719/94‑1 (MQ=60)

                                           TTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTC                                                <  SRR4302534.331519/94‑1 (MQ=60)

                                           TTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTC                                                <  SRR4302534.4941174/94‑1 (MQ=60)

                                           TTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTC                                                <  SRR4302534.5576739/94‑1 (MQ=60)

                                            TTGCCGCGCATGGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACATGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCG                                               <  SRR4302534.856610/94‑1 (MQ=60)

                                            TTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCG                                               <  SRR4302534.2317606/94‑1 (MQ=60)

                                            TTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCG                                               <  SRR4302534.5381690/94‑1 (MQ=60)

                                             TGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGC                                              <  SRR4302534.5670918/94‑1 (MQ=60)

                                                CGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCT                                           <  SRR4302534.6433830/94‑1 (MQ=60)

                                                CGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCT                                           >  SRR4302534.1749584/1‑94 (MQ=60)

                                                 GCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTC                                          <  SRR4302534.6108080/94‑1 (MQ=60)

                                                  CGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCC                                         >  SRR4302534.4224460/1‑94 (MQ=60)

                                                  CGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCC                                         <  SRR4302534.2450019/94‑1 (MQ=60)

                                                   GCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCG                                        <  SRR4302534.1380795/94‑1 (MQ=60)

                                                    CATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGC                                       >  SRR4302534.643830/1‑94 (MQ=60)

                                                    CATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGC                                       <  SRR4302534.432725/94‑1 (MQ=60)

                                                    CATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGC                                       <  SRR4302534.2876045/94‑1 (MQ=60)

                                                    CATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGC                                       >  SRR4302534.3892831/1‑94 (MQ=60)

                                                    CATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGC                                       >  SRR4302534.6066119/1‑94 (MQ=60)

                                                     ATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCT                                      >  SRR4302534.1132605/1‑94 (MQ=60)

                                                     ATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCT                                      >  SRR4302534.1280938/1‑94 (MQ=60)

                                                     ATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCT                                      >  SRR4302534.2874759/1‑94 (MQ=60)

                                                     ATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCT                                      >  SRR4302534.4154690/1‑94 (MQ=60)

                                                     ATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCT                                      <  SRR4302534.4105953/94‑1 (MQ=60)

                                                     ATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCT                                      >  SRR4302534.3422471/1‑94 (MQ=60)

                                                      TCGTCGATGTCATGTTCCGAGATCGGATTCACCGCCCCGTGGTTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTG                                     <  SRR4302534.3277954/94‑1 (MQ=60)

                                                      TCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTG                                     <  SRR4302534.3069303/94‑1 (MQ=60)

                                                       CGTCGATGTGACGTTCCGAGAAGGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTGGCGCTGCGCTGA                                    >  SRR4302534.2951761/1‑94 (MQ=60)

                                                       CGTCGATGTCACTTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGA                                    <  SRR4302534.2787680/94‑1 (MQ=60)

                                                       CGTCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGA                                    >  SRR4302534.4718760/1‑94 (MQ=60)

                                                         TCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAA                                  <  SRR4302534.4804204/94‑1 (MQ=60)

                                                         TCGATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAA                                  <  SRR4302534.301506/94‑1 (MQ=60)

                                                           GATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACC                                <  SRR4302534.1167925/94‑1 (MQ=60)

                                                           GATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACC                                <  SRR4302534.1552664/94‑1 (MQ=60)

                                                            ATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCT                               >  SRR4302534.1951177/1‑94 (MQ=60)

                                                            ATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCT                               <  SRR4302534.804953/94‑1 (MQ=60)

                                                            ATGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCT                               >  SRR4302534.1969787/1‑94 (MQ=60)

                                                             TGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTG                              >  SRR4302534.2025474/1‑94 (MQ=60)

                                                             TGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTG                              >  SRR4302534.2966191/1‑94 (MQ=60)

                                                             TGTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTG                              >  SRR4302534.1651198/1‑94 (MQ=60)

                                                              taCACGTTCCGAGAACGGATGCGCCGCCACCTGGCTGCCTTTCAAGTAACGTGGATAGTTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGG                             <  SRR4302534.2043198/92‑1 (MQ=60)

                                                              GTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGG                             <  SRR4302534.6184083/94‑1 (MQ=60)

                                                              GTCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGG                             >  SRR4302534.4052209/1‑94 (MQ=60)

                                                               TCACGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGGG                            >  SRR4302534.210236/1‑94 (MQ=60)

                                                                 CCGTTCCGAGAACGCAGTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTGAAACAGACGGTCGCGCTCCGCTGAAACCTGGGTG                          <  SRR4302534.4963145/94‑1 (MQ=60)

                                                                  CGTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGGGTGG                         >  SRR4302534.5484877/1‑94 (MQ=60)

                                                                   GTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGGGTGGT                        <  SRR4302534.2001329/94‑1 (MQ=60)

                                                                   GTTCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGGGTGGT                        <  SRR4302534.3629506/94‑1 (MQ=60)

                                                                     TCCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGGGTGGTGT                      <  SRR4302534.6149397/94‑1 (MQ=60)

                                                                      CCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGGGTGGTGTT                     >  SRR4302534.621341/1‑94 (MQ=60)

                                                                      CCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGGGTGGTGTT                     <  SRR4302534.3103323/94‑1 (MQ=60)

                                                                      CCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGGGTGGTGTT                     <  SRR4302534.968559/94‑1 (MQ=60)

                                                                      CCGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGGGTGGTGTT                     <  SRR4302534.4192198/94‑1 (MQ=60)

                                                                       CGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGGGTGGTGTTG                    >  SRR4302534.1584887/1‑94 (MQ=60)

                                                                       CGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGGGTGGTGTTG                    >  SRR4302534.4393507/1‑94 (MQ=60)

                                                                       CGAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGGGTGGTGTTG                    >  SRR4302534.1302324/1‑94 (MQ=60)

                                                                        GAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGGGTGGTGTTGG                   <  SRR4302534.3204822/94‑1 (MQ=60)

                                                                        GAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGGGTGGTGTTGG                   >  SRR4302534.4226210/1‑94 (MQ=60)

                                                                        GAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGGGTGGTGTTGG                   >  SRR4302534.1181851/1‑94 (MQ=60)

                                                                        GAGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGGGTGGTGTTGG                   <  SRR4302534.6260057/94‑1 (MQ=60)

                                                                         AGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGGGTGGTGTTGGC                  <  SRR4302534.3206770/94‑1 (MQ=60)

                                                                         AGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGGGTGGTGTTGGC                  >  SRR4302534.5744810/1‑94 (MQ=60)

                                                                         AGAACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGGGTGGTGTTGGC                  <  SRR4302534.2190995/94‑1 (MQ=60)

                                                                           AACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGGGTGGTGTTGGCCT                >  SRR4302534.1677879/1‑94 (MQ=60)

                                                                           AACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGGGTGGTGTTGGCCT                <  SRR4302534.5987445/94‑1 (MQ=60)

                                                                           AACGGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGGGTGGTGTTGGCCT                <  SRR4302534.4769510/94‑1 (MQ=60)

                                                                              GGATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGGGTGGTGTTGGCCTGCG             <  SRR4302534.5511678/94‑1 (MQ=60)

                                                                                ATTCACCGCCACCTGGCTGCCTTTCAAGTAACGTTAATAATTTTTAAACAGACGTTCGCGCTCCGCTGAAACCTGGGTGGTGTTGACCTGCGCT           <  SRR4302534.84294/94‑1 (MQ=60)

                                                                                  TCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGGGTGGTGTTGGCCTGCGCTTC         <  SRR4302534.2338475/94‑1 (MQ=60)

                                                                                  TCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGGGTGGTGTTGGCCTGCGCTTC         >  SRR4302534.2180447/1‑94 (MQ=60)

                                                                                  TCACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGGGTGGTGTTGGCCTGCGCTTC         >  SRR4302534.5695349/1‑94 (MQ=60)

                                                                                  TCACCGCCACCTGGCTGCCTTGCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGGGTGGTGTTGGACTGCGCTTC         >  SRR4302534.6383549/1‑94 (MQ=60)

                                                                                   tcCCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGGGTGGTGTTGGCCTGCGCTTCA        <  SRR4302534.4488908/92‑1 (MQ=60)

                                                                                   CACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGGGTGGTGTTGGCCTGCGCTTCA        <  SRR4302534.1408613/94‑1 (MQ=60)

                                                                                    ACCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGGGTGGTGTTGGCCTGCGCTTCAG       >  SRR4302534.3315204/1‑94 (MQ=60)

                                                                                     CCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGGGTGGTGTTGGCCTGCGCTTCAGT      <  SRR4302534.2796543/94‑1 (MQ=60)

                                                                                     CCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGGGTGGTGTTGGCCTGCGCTTCAGT      <  SRR4302534.5738524/94‑1 (MQ=60)

                                                                                     CCGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGGGTGGTGTTGGCCTGCGCTTCAGT      <  SRR4302534.4252861/94‑1 (MQ=60)

                                                                                      CGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGGGTGGTGTTGGCCTGCGCTTCAGTG     >  SRR4302534.3538660/1‑94 (MQ=60)

                                                                                      CGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGGGTGGTGTTGGCCTGCGCTTCAGTG     >  SRR4302534.377435/1‑94 (MQ=60)

                                                                                      CGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGGGTGGTGTTGGCCTGCGCTTCAGTG     <  SRR4302534.488421/94‑1 (MQ=60)

                                                                                      CGCCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGGGTGGTGTTGGCCTGCGCTTCAGTG     <  SRR4302534.4922396/94‑1 (MQ=60)

                                                                                        CCACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGGGTGGTGTTGGCCTGCGCTTCAGTGAG   >  SRR4302534.1583684/1‑94 (MQ=60)

                                                                                         CACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGGGTGGTGTTGGCCTGCGCTTCAGTGAGC  >  SRR4302534.2248954/1‑94 (MQ=60)

                                                                                         CACCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGGGTGGTGTTGGCCTGCGCTTCAGTGAGC  >  SRR4302534.4583041/1‑94 (MQ=60)

TCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGGGTGGTGTTGGCCTGCGCTTCAGTGAGC  >  NC_000913/3768456‑3768638

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`