Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	884	257.2	12958910	96.9%	12557183	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,945,351	+C	coding (1/600 nt) coding (240/240 nt)	yifDA ← yifDA ←	transcription regulator transcription regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,945,350	1	.	C	99.6%	825.3 / ‑4.3	234	coding (2/600 nt)	yifDA	transcription regulator
Reads supporting (aligned to +/- strand): ref base . (0/1); new base C (111/122); total (111/123)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 254 total aligned reads displayed.

ACAAGCGTCCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCT‑CTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCT  >  NC_000913/3945258‑3945437

  aaGCGTCCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACct‑cc                                                                                       >  1:101826/1‑92 (MQ=255)

  aaGCGTCCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACct‑cc                                                                                       <  2:4024101/93‑2 (MQ=255)

  aaGCGTCCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACct‑cc                                                                                       >  2:1360874/1‑92 (MQ=255)

  aaGCGTCCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACct‑cc                                                                                       >  1:2064123/1‑92 (MQ=255)

   aGCGTCCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACct‑cct                                                                                      <  1:4638335/93‑3 (MQ=255)

     cGTCCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACct‑cctta                                                                                    >  2:680864/1‑89 (MQ=255)

        cccAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACgg                                                                                  <  1:4422391/93‑1 (MQ=255)

        cccAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACgg                                                                                  >  2:2858011/1‑93 (MQ=255)

         ccAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACggg                                                                                 >  1:5987693/1‑93 (MQ=255)

         ccAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACggg                                                                                 >  1:59794/1‑93 (MQ=255)

              cACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGcc                                                                            <  2:2643351/93‑1 (MQ=255)

              cACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGcc                                                                            >  2:1711796/1‑93 (MQ=255)

               aCTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCt                                                                           >  1:2293781/1‑93 (MQ=255)

               aCTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCt                                                                           >  1:3777712/1‑93 (MQ=255)

                 tGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGc                                                                         >  2:359303/1‑93 (MQ=255)

                  gATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGcc                                                                        <  1:1805324/93‑1 (MQ=255)

                   aTTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCa                                                                       >  2:1072018/1‑93 (MQ=255)

                    ttAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAg                                                                      <  1:3528710/93‑1 (MQ=255)

                      aaGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGt                                                                    >  1:3201384/1‑93 (MQ=255)

                       aGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTg                                                                   >  1:3297819/1‑93 (MQ=255)

                        gCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGc                                                                  >  2:3781386/1‑93 (MQ=255)

                        gCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGc                                                                  >  2:768243/1‑93 (MQ=255)

                         cATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCt                                                                 >  1:1088236/1‑93 (MQ=255)

                         cATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCt                                                                 <  1:3467096/93‑1 (MQ=255)

                          aTACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTc                                                                <  2:3005850/93‑1 (MQ=255)

                           tACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCa                                                               >  1:5435276/1‑93 (MQ=255)

                           tACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCa                                                               <  2:4292358/93‑1 (MQ=255)

                            aCATTCCCACAGCGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCTCCTCCTTACGGGCGGCCTGCCAGGTGCTCAt                                                              <  1:550094/93‑1 (MQ=255)

                            aCATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCAt                                                              <  1:496643/93‑1 (MQ=255)

                             cATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATg                                                             >  2:1954466/1‑93 (MQ=255)

                             cATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATg                                                             <  1:4218374/93‑1 (MQ=255)

                              aTTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGa                                                            <  1:3036723/93‑1 (MQ=255)

                               ttCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAg                                                           <  2:4665536/93‑1 (MQ=255)

                               ttCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAg                                                           <  1:1671810/93‑1 (MQ=255)

                                  ccACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGt                                                        >  2:4599163/1‑93 (MQ=255)

                                  ccACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGt                                                        <  2:2235561/93‑1 (MQ=255)

                                   gacaACGAGGGGCTGGCACCGATAGAAAACTCGTTATGGCGTTAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGtt                                                       >  1:1599594/2‑93 (MQ=255)

                                   cacaACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGtt                                                       <  1:3104989/93‑1 (MQ=255)

                                   cacaACGAGGCGCTAGCACCGATAGAAAACTCGTTATGTCGTGAGGAATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGtt                                                       >  1:2243956/1‑93 (MQ=255)

                                    acaACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGttt                                                      >  2:6165860/1‑93 (MQ=255)

                                     caACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGTGCGGCCTGCCAGGTGCTCATGAGCGTTTc                                                     >  1:6352298/1‑93 (MQ=255)

                                     caACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTc                                                     <  1:641965/93‑1 (MQ=255)

                                     caACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTc                                                     <  2:3634864/93‑1 (MQ=255)

                                      aaCGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCt                                                    <  1:1258837/93‑1 (MQ=255)

                                      aaCGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCt                                                    >  2:3545772/1‑93 (MQ=255)

                                        cGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGc                                                  >  2:5335315/1‑93 (MQ=255)

                                        cGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGc                                                  >  2:5440380/1‑93 (MQ=255)

                                         gAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCa                                                 >  1:5197284/1‑93 (MQ=255)

                                          aGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCAt                                                <  1:5052756/93‑1 (MQ=255)

                                          aGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCAt                                                >  2:2624602/1‑93 (MQ=255)

                                           ggCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATa                                               >  1:2459105/1‑93 (MQ=255)

                                           ggCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATa                                               >  2:1448582/1‑93 (MQ=255)

                                            gcgcTGGCACTGATAGAAACCTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATaa                                              <  1:405395/93‑1 (MQ=255)

                                            gcgcTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATaa                                              <  2:2169461/93‑1 (MQ=255)

                                            gcgcTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATaa                                              <  1:1373128/93‑1 (MQ=255)

                                               cTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGc                                           >  1:2909744/1‑93 (MQ=255)

                                               cTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGc                                           <  2:4832015/93‑1 (MQ=255)

                                               cTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGc                                           >  2:3318130/1‑93 (MQ=255)

                                                 ggCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCag                                         >  2:1930917/1‑93 (MQ=255)

                                                    aCCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCagtag                                      >  1:6189210/1‑93 (MQ=255)

                                                     ccGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCagtagt                                     >  2:4757120/1‑93 (MQ=255)

                                                     ccGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCagtagt                                     <  1:5174992/93‑1 (MQ=255)

                                                      cGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGtt                                    <  1:2623918/93‑1 (MQ=255)

                                                       gATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGttt                                   <  1:3805171/93‑1 (MQ=255)

                                                         tAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGttttt                                 <  2:5714324/93‑1 (MQ=255)

                                                         tAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGttttt                                 >  2:5699587/1‑93 (MQ=255)

                                                          aGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTc                                >  2:1636378/1‑93 (MQ=255)

                                                          aGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTc                                >  2:55635/1‑93 (MQ=255)

                                                          aGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTc                                >  1:4606845/1‑93 (MQ=255)

                                                               aCTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCgg                           >  1:854878/1‑93 (MQ=255)

                                                                 tCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCa                         <  1:4836317/93‑1 (MQ=255)

                                                                   gTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGc                       <  2:6272959/93‑1 (MQ=255)

                                                                   cttATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGc                       <  1:5334128/92‑1 (MQ=255)

                                                                     tATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCgg                     <  2:2363226/93‑1 (MQ=255)

                                                                      aTGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGt                    <  2:80237/93‑1 (MQ=255)

                                                                       tGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGtt                   <  1:6121154/93‑1 (MQ=255)

                                                                       tGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGtt                   <  2:6029396/93‑1 (MQ=255)

                                                                       tGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGtt                   <  1:726441/93‑1 (MQ=255)

                                                                        gTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTa                  <  2:4228693/93‑1 (MQ=255)

                                                                        gTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTa                  >  1:720389/1‑93 (MQ=255)

                                                                          cGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTaaa                <  2:4554477/93‑1 (MQ=255)

                                                                           gTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAAc               >  2:4992184/1‑93 (MQ=255)

                                                                             gAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACgg             >  2:1248279/1‑93 (MQ=255)

                                                                              aGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGa            <  1:4860779/93‑1 (MQ=255)

                                                                              aGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGa            <  1:333792/93‑1 (MQ=255)

                                                                               ggTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACggct           >  2:243048/1‑91 (MQ=255)

                                                                               ggTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGat           <  2:3913328/93‑1 (MQ=255)

                                                                               ggTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGat           >  1:3964174/1‑93 (MQ=255)

                                                                                gTATGCGCCACCTCCTTACGGGCGGGCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGata          <  2:6329277/93‑1 (MQ=255)

                                                                                gTATGCGCCACCTCCTTACGGGCGGCCTGCCAGTTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGata          <  2:1791977/93‑1 (MQ=255)

                                                                                gTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGata          >  1:1178927/1‑93 (MQ=255)

                                                                                 tATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGatat         >  2:2007790/1‑93 (MQ=255)

                                                                                 tATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGatat         <  1:5619541/93‑1 (MQ=255)

                                                                                 tATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGatat         <  2:5853074/93‑1 (MQ=255)

                                                                                 tATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGatat         >  2:4126422/1‑93 (MQ=255)

                                                                                    gcgcCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGt      <  2:933218/93‑1 (MQ=255)

                                                                                     cgcCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGtt     >  1:4489900/1‑93 (MQ=255)

                                                                                     cgcCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGtt     <  2:2717579/93‑1 (MQ=255)

                                                                                      gcCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGttt    >  1:5588580/1‑93 (MQ=255)

                                                                                       ccACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTc   <  2:2438129/93‑1 (MQ=255)

ACAAGCGTCCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCT‑CTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCT  >  NC_000913/3945258‑3945437

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

ACAAGCGTCCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCT‑CTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCG  >  NC_000913/3945258‑3945443

ACAAGCGTCCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCT‑C                                                                                              <  SRR4302534.3471720/94‑1 (MQ=60)

 CAAGCGTCCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCT‑CC                                                                                             >  SRR4302534.2118097/1‑94 (MQ=60)

 CAAGCGTCCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCT‑CC                                                                                             >  SRR4302534.104542/1‑94 (MQ=60)

 CAAGCGTCCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCT‑CC                                                                                             <  SRR4302534.4426047/94‑1 (MQ=60)

  AAGCGTCCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCT‑CCT                                                                                            <  SRR4302534.3413638/94‑1 (MQ=60)

   AGCGTCCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCT‑Cctt                                                                                           <  SRR4302534.4764189/94‑4 (MQ=60)

      GTCCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACG                                                                                         >  SRR4302534.5227614/1‑94 (MQ=60)

      GTCCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACG                                                                                         <  SRR4302534.2776656/94‑1 (MQ=60)

      GTCCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACG                                                                                         <  SRR4302534.4259135/94‑1 (MQ=60)

      CTCCCAGCCACTTATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACGCGTTATGTCGTGAGGTATGCGCCGCCTCCTTACG                                                                                         <  SRR4302534.2783979/94‑1 (MQ=60)

        CCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGG                                                                                       >  SRR4302534.61412/1‑94 (MQ=60)

        CCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGG                                                                                       <  SRR4302534.4541794/94‑1 (MQ=60)

        CCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGG                                                                                       >  SRR4302534.6150346/1‑94 (MQ=60)

         CCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGC                                                                                      <  SRR4302534.4969418/94‑1 (MQ=60)

          CAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCG                                                                                     <  SRR4302534.6290794/94‑1 (MQ=60)

           AGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGG                                                                                    >  SRR4302534.1634996/1‑94 (MQ=60)

           AGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGG                                                                                    >  SRR4302534.4164426/1‑94 (MQ=60)

              CACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCT                                                                                 >  SRR4302534.4769367/1‑94 (MQ=60)

              CACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCT                                                                                 >  SRR4302534.3879128/1‑94 (MQ=60)

              CACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCT                                                                                 >  SRR4302534.2354663/1‑94 (MQ=60)

              CACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCT                                                                                 >  SRR4302534.2303733/1‑94 (MQ=60)

                  GATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCA                                                                             <  SRR4302534.1852195/94‑1 (MQ=60)

                  GATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCA                                                                             >  SRR4302534.2442026/1‑94 (MQ=60)

                    TTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGG                                                                           <  SRR4302534.3623624/94‑1 (MQ=60)

                    TTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGG                                                                           <  SRR4302534.6519994/94‑1 (MQ=60)

                     TAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGT                                                                          >  SRR4302534.3286272/1‑94 (MQ=60)

                      AAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTG                                                                         >  SRR4302534.3385815/1‑94 (MQ=60)

                      AAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTG                                                                         >  SRR4302534.3383100/1‑94 (MQ=60)

                        GCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCT                                                                       >  SRR4302534.1116541/1‑94 (MQ=60)

                        GCATACATTCCCACAACGAGGCACTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCT                                                                       <  SRR4302534.5106790/94‑1 (MQ=60)

                         CATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTC                                                                      >  SRR4302534.3502589/1‑94 (MQ=60)

                         CATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTC                                                                      <  SRR4302534.3560282/94‑1 (MQ=60)

                         CATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTC                                                                      <  SRR4302534.2354269/94‑1 (MQ=60)

                         CATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTC                                                                      >  SRR4302534.5397108/1‑94 (MQ=60)

                          ATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCA                                                                     >  SRR4302534.5581634/1‑94 (MQ=60)

                            ACATTCCCACAGCGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCTCCTCCTTACGGGCGGCCTGCCAGGTGCTCATG                                                                   <  SRR4302534.563656/94‑1 (MQ=60)

                            ACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATG                                                                   <  SRR4302534.509032/94‑1 (MQ=60)

                             CATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCGCCTCCTTACGGGCGGCCTGCCAGGTGCTCATGA                                                                  <  SRR4302534.6573527/94‑1 (MQ=60)

                             CATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGA                                                                  <  SRR4302534.4332115/94‑1 (MQ=60)

                             CATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGA                                                                  >  SRR4302534.4393287/1‑94 (MQ=60)

                             CATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGA                                                                  <  SRR4302534.6146316/94‑1 (MQ=60)

                              ATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAG                                                                 <  SRR4302534.1103190/94‑1 (MQ=60)

                              ATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAG                                                                 <  SRR4302534.3116353/94‑1 (MQ=60)

                               TTCCCACGACGACGCGCTGTCACCGATAGAAAACTCGTTATTTCGTGAGGTATGCGCCGCCTCCTTACGGGCGACCTGCCAGGTGCTCATGAGC                                                                <  SRR4302534.550741/94‑1 (MQ=60)

                               TTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGC                                                                <  SRR4302534.3072585/94‑1 (MQ=60)

                               TTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGC                                                                <  SRR4302534.1715318/94‑1 (MQ=60)

                                 CCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGT                                                              <  SRR4302534.262258/94‑1 (MQ=60)

                                  CGACAACGAGGGGCTGGCACCGATAGAAAACTCGTTATGGCGTTAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTT                                                             >  SRR4302534.1641384/1‑94 (MQ=60)

                                  CCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTT                                                             <  SRR4302534.470971/94‑1 (MQ=60)

                                  CCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTT                                                             <  SRR4302534.387861/94‑1 (MQ=60)

                                  CCACAACGAGGCGCTAGCACCGATAGAAAACTCGTTATGTCGTGAGGAATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTT                                                             >  SRR4302534.2303426/1‑94 (MQ=60)

                                   CACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTT                                                            <  SRR4302534.3186650/94‑1 (MQ=60)

                                   CACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTT                                                            <  SRR4302534.187830/94‑1 (MQ=60)

                                    ACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGTGCGGCCTGCCAGGTGCTCATGAGCGTTTC                                                           >  SRR4302534.6523848/1‑94 (MQ=60)

                                     CAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCT                                                          <  SRR4302534.657539/94‑1 (MQ=60)

                                      AACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTG                                                         <  SRR4302534.1291997/94‑1 (MQ=60)

                                      AACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTG                                                         <  SRR4302534.1973441/94‑1 (MQ=60)

                                       ACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGC                                                        <  SRR4302534.3233386/94‑1 (MQ=60)

                                        CGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCA                                                       <  SRR4302534.1691216/94‑1 (MQ=60)

                                        CGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCA                                                       >  SRR4302534.5337450/1‑94 (MQ=60)

                                        CGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCA                                                       <  SRR4302534.2699750/94‑1 (MQ=60)

                                         GAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCAT                                                      >  SRR4302534.1192634/1‑94 (MQ=60)

                                         GAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCAT                                                      >  SRR4302534.4570146/1‑94 (MQ=60)

                                         GAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCAT                                                      >  SRR4302534.3297990/1‑94 (MQ=60)

                                         GAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCAT                                                      >  SRR4302534.3604392/1‑94 (MQ=60)

                                         GAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCAT                                                      >  SRR4302534.3975907/1‑94 (MQ=60)

                                          AGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATA                                                     >  SRR4302534.2524436/1‑94 (MQ=60)

                                          AGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATA                                                     <  SRR4302534.5189211/94‑1 (MQ=60)

                                            GCGCTGGCACTGATAGAAACCTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAG                                                   <  SRR4302534.415754/94‑1 (MQ=60)

                                            GCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAG                                                   <  SRR4302534.1409310/94‑1 (MQ=60)

                                             CGCTGGCACCGATAGAAAACACGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGG                                                  >  SRR4302534.6409057/1‑94 (MQ=60)

                                              GCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGC                                                 <  SRR4302534.2931227/94‑1 (MQ=60)

                                              GCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGC                                                 >  SRR4302534.2986127/1‑94 (MQ=60)

                                                TGGCACCGATAGAAAACTCGTTATGTCATGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAG                                               <  SRR4302534.1734925/94‑1 (MQ=60)

                                                 GGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGT                                              >  SRR4302534.1703548/1‑94 (MQ=60)

                                                  GCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTA                                             >  SRR4302534.4059716/1‑94 (MQ=60)

                                                   CACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAG                                            >  SRR4302534.6357641/1‑94 (MQ=60)

                                                    ACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGT                                           >  SRR4302534.3185586/1‑94 (MQ=60)

                                                     CCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTT                                          <  SRR4302534.5657778/94‑1 (MQ=60)

                                                     CCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTT                                          >  SRR4302534.759365/1‑94 (MQ=60)

                                                     CCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTT                                          <  SRR4302534.5314450/94‑1 (MQ=60)

                                                      CGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTT                                         <  SRR4302534.2693333/94‑1 (MQ=60)

                                                       GATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCGGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTT                                        <  SRR4302534.5317384/94‑1 (MQ=60)

                                                       GATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTT                                        >  SRR4302534.4690672/1‑94 (MQ=60)

                                                       GATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTT                                        >  SRR4302534.4760255/1‑94 (MQ=60)

                                                       GATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTT                                        <  SRR4302534.3907342/94‑1 (MQ=60)

                                                         TAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTC                                      >  SRR4302534.4731862/1‑94 (MQ=60)

                                                          AGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATTAGGCAGTAGTTTTTCA                                     >  SRR4302534.5585288/1‑94 (MQ=60)

                                                           GAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCAC                                    >  SRR4302534.5457799/1‑94 (MQ=60)

                                                            AAAGCTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGACCTGCTAGGTGCTCATGAGCGTTTCTGGATAAGGCAGTAGTTTTTCACC                                   <  SRR4302534.800980/94‑1 (MQ=60)

                                                            AAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACC                                   <  SRR4302534.1239882/94‑1 (MQ=60)

                                                            AAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACC                                   >  SRR4302534.2163900/1‑94 (MQ=60)

                                                             AAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCG                                  >  SRR4302534.1300756/1‑94 (MQ=60)

                                                             AAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCG                                  <  SRR4302534.5757908/94‑1 (MQ=60)

                                                              AACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGG                                 <  SRR4302534.372767/94‑1 (MQ=60)

                                                              AACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGG                                 >  SRR4302534.3153226/1‑94 (MQ=60)

                                                              AACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGG                                 >  SRR4302534.876449/1‑94 (MQ=60)

                                                               ACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGC                                >  SRR4302534.3811290/1‑94 (MQ=60)

                                                                 TCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAG                              <  SRR4302534.4967528/94‑1 (MQ=60)

                                                                  CGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGC                             <  SRR4302534.135604/94‑1 (MQ=60)

                                                                  CGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGC                             >  SRR4302534.6032007/1‑94 (MQ=60)

                                                                   CTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCG                            <  SRR4302534.5477973/94‑1 (MQ=60)

                                                                    TTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGG                           >  SRR4302534.4875779/1‑94 (MQ=60)

                                                                       TGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTA                        <  SRR4302534.6287646/94‑1 (MQ=60)

                                                                       TGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTA                        >  SRR4302534.738162/1‑94 (MQ=60)

                                                                       TGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTA                        <  SRR4302534.744373/94‑1 (MQ=60)

                                                                       TGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTA                        <  SRR4302534.2526981/94‑1 (MQ=60)

                                                                          CGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAAC                     >  SRR4302534.6284762/1‑94 (MQ=60)

                                                                           GTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACG                    <  SRR4302534.114454/94‑1 (MQ=60)

                                                                             GAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGA                  >  SRR4302534.6233879/1‑94 (MQ=60)

                                                                             GAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGA                  <  SRR4302534.887826/94‑1 (MQ=60)

                                                                             GAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGA                  >  SRR4302534.5082340/1‑94 (MQ=60)

                                                                             GAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGA                  <  SRR4302534.5565393/94‑1 (MQ=60)

                                                                              AGGTATGCGCCGCCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGAT                 <  SRR4302534.526576/94‑1 (MQ=60)

                                                                              AGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGAT                 <  SRR4302534.6046416/94‑1 (MQ=60)

                                                                              AGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGAT                 <  SRR4302534.4992649/94‑1 (MQ=60)

                                                                              AGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGAT                 >  SRR4302534.4070303/1‑94 (MQ=60)

                                                                              AGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGAT                 <  SRR4302534.342357/94‑1 (MQ=60)

                                                                               GGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATA                <  SRR4302534.703127/94‑1 (MQ=60)

                                                                               GGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATA                >  SRR4302534.1209915/1‑94 (MQ=60)

                                                                                GTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATAT               <  SRR4302534.4205431/94‑1 (MQ=60)

                                                                                 TATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATT              <  SRR4302534.5771585/94‑1 (MQ=60)

                                                                                 TATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATT              >  SRR4302534.695767/1‑94 (MQ=60)

                                                                                  ATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTG             <  SRR4302534.5664770/94‑1 (MQ=60)

                                                                                    GCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTT           >  SRR4302534.4611395/1‑94 (MQ=60)

                                                                                    GCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTT           <  SRR4302534.4131496/94‑1 (MQ=60)

                                                                                     CGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTT          >  SRR4302534.5739642/1‑94 (MQ=60)

                                                                                       ccccctcCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCT        <  SRR4302534.4583102/87‑1 (MQ=60)

                                                                                       CCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCT        <  SRR4302534.5630038/94‑1 (MQ=60)

                                                                                       CCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGATTTTCACCGGCAGCGGTTAAACGGATATTGTTTCT        >  SRR4302534.741087/1‑94 (MQ=60)

                                                                                        CACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTG       >  SRR4302534.12029/1‑94 (MQ=60)

                                                                                          CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTG     <  SRR4302534.4112033/94‑1 (MQ=60)

                                                                                          CCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTG     <  SRR4302534.5158913/94‑1 (MQ=60)

                                                                                           ctcCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGG    <  SRR4302534.6265188/91‑1 (MQ=60)

                                                                                            C‑CTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCG  <  SRR4302534.2921262/94‑1 (MQ=60)

ACAAGCGTCCCAGCCACTGATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCT‑CTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCG  >  NC_000913/3945258‑3945443

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 10 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`