Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	884	257.2	12958910	96.9%	12557183	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,986,029	A→G	R217R (CGT→CGC)	hemX ←	uroporphyrinogen III methylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,986,029	0	A	G	99.6%	879.6 / NA	253	R217R (CGT→CGC)	hemX	uroporphyrinogen III methylase
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (129/123); minor base T (0/1); total (129/124)
Fisher's exact test for biased strand distribution p-value = 4.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 256 total aligned reads displayed.

TGCAGATTGATACGCCATTCGCTGATGGAACTGGAAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGACGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCAGAAAGGCTGGCGAT  >  NC_000913/3985940‑3986118

tGCAGATTGATACGCCATTCGCTGATGGAACTGGAAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGgcgc                                                                                        >  2:6140875/1‑93 (MQ=255)

 gCAGATTGATACGCCATTCGCTGATGGAACTGGAAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCa                                                                                       <  2:2470865/93‑1 (MQ=255)

  cAGATTGATACGCCATTCGCTGATGGAACTGGAAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAg                                                                                      >  2:481514/1‑93 (MQ=255)

  cAGATTGATACGCCATTCGCTGATGGAACTGGAAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAg                                                                                      <  1:3614605/93‑1 (MQ=255)

  cAGATTGATACGCCATTCGCTGATGGAACTGGAAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAg                                                                                      <  1:6247691/93‑1 (MQ=255)

  cAGATTGATACGCCATTCGCTGATGGAACTGGAAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAg                                                                                      <  1:727891/93‑1 (MQ=255)

  cAGATTGATACGCCATTCGCTGATGGAACTGGAAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAg                                                                                      <  1:1268190/93‑1 (MQ=255)

   aGATTGATACGCCATTCGCTGATGGAACTGGAAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAgg                                                                                     >  2:794634/1‑93 (MQ=255)

   aGATTGATACGCCATTCGCTGATGGAACTGGAAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAgg                                                                                     >  1:984047/1‑93 (MQ=255)

    gATTGATACGCCATTCGCTGATGGAACTGGAAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGt                                                                                    >  1:1708322/1‑93 (MQ=255)

    gATTGATACGCCATTCGCTGATGGAACTGGAAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGt                                                                                    >  1:4170960/1‑93 (MQ=255)

     aTTGATACGCCATTCGCTGATGGAACTGGAAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCCGGtt                                                                                   >  2:5612112/1‑93 (MQ=255)

      ttGATACGCCATTCGCTGATGGAACTGGAAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTa                                                                                  >  2:3978535/1‑93 (MQ=255)

       tGATACGCCATTCGCTGATGGAACTGGAAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTAt                                                                                 >  1:4887891/1‑93 (MQ=255)

        gATACGCCATTCGCTGATGGAACTGGAAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATc                                                                                >  2:6196741/1‑93 (MQ=255)

        gATACGCCATTCGCTGATGGAACTGGAAAGCTCTACACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATc                                                                                <  2:520903/93‑1 (MQ=255)

         aTACGCCATTCGCTGATGGAACTGGAAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCt                                                                               >  1:5985779/1‑93 (MQ=255)

             gCCATTCGCTGATGGAACTGGAAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCt                                                                           <  2:2625821/93‑1 (MQ=255)

             gCCATTCGCTGATGGAACTGGAAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCt                                                                           >  2:5137557/1‑93 (MQ=255)

              ccATTCGCTGATGGAACTGGAAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTg                                                                          >  2:5965439/1‑93 (MQ=255)

              ccATTCGCTGATGGAACTGGAAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTg                                                                          >  2:6412120/1‑93 (MQ=255)

               cATTCGCTGATGGAACTGGAAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGa                                                                         >  1:2494524/1‑93 (MQ=255)

               cATTCGCTGATGGAACTGGAAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGa                                                                         <  2:4402668/93‑1 (MQ=255)

                aTTCGCTGATGGAACTGGAAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGAt                                                                        >  2:3610883/1‑93 (MQ=255)

                aTTCGCTGATGGAACTGGAAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGAt                                                                        >  1:608973/1‑93 (MQ=255)

                 ttCGCTGATGGAACTGGAAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGAtt                                                                       >  1:4652325/1‑93 (MQ=255)

                   cGCTGATGGAACTGGAAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTg                                                                     >  2:1349115/1‑93 (MQ=255)

                   cGCTGATGGAACTGGAAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTg                                                                     >  2:5870658/1‑93 (MQ=255)

                    gCTGATGGAACTGGAAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGa                                                                    >  2:3520830/1‑93 (MQ=255)

                    gCTGATGGAACTGGAAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGa                                                                    >  2:6300597/1‑93 (MQ=255)

                    gCTGATGGAACTGGAAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGa                                                                    <  2:2036151/93‑1 (MQ=255)

                     cTGATGGAACTGGAAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGaa                                                                   >  2:5184325/1‑93 (MQ=255)

                       gATGGAACTGGAAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAg                                                                 >  1:3452568/1‑93 (MQ=255)

                       gATGGAACTGGAAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAg                                                                 >  2:341682/1‑93 (MQ=255)

                       gATGGAACTGGAAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAg                                                                 >  1:6285284/1‑93 (MQ=255)

                        aTGGAACTGGAAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGc                                                                <  1:4965663/93‑1 (MQ=255)

                          ggAACTGGAAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTg                                                              >  1:2960423/1‑93 (MQ=255)

                           gAACTGGAAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGa                                                             <  1:590675/93‑1 (MQ=255)

                            aaCTGGAAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGAt                                                            <  1:1361870/93‑1 (MQ=255)

                            aaCTGGAAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGAt                                                            <  1:4361372/93‑1 (MQ=255)

                               tGGAAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTaa                                                         >  2:3266162/1‑93 (MQ=255)

                               tGGAAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTaa                                                         >  1:5713137/1‑93 (MQ=255)

                               tGGAAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTaa                                                         <  2:4713890/93‑1 (MQ=255)

                                  aaaGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCt                                                      <  2:752882/93‑1 (MQ=255)

                                   aaGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCtt                                                     >  2:2968228/1‑93 (MQ=255)

                                   aaGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCtt                                                     >  1:1833258/1‑93 (MQ=255)

                                   aaGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCtt                                                     >  1:3823703/1‑93 (MQ=255)

                                    aGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTa                                                    <  1:241464/93‑1 (MQ=255)

                                    aGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTa                                                    >  1:3882757/1‑93 (MQ=255)

                                    aGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTa                                                    >  2:5574390/1‑93 (MQ=255)

                                    aGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTa                                                    >  1:1000722/1‑93 (MQ=255)

                                     gCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTaa                                                   <  2:846945/93‑1 (MQ=255)

                                       tctTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAgg                                                 <  2:1182529/93‑1 (MQ=255)

                                       tctTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAgg                                                 >  2:5261407/1‑93 (MQ=255)

                                        ctTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGga                                                <  2:2048861/93‑1 (MQ=255)

                                        ctTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGga                                                >  1:2765275/1‑93 (MQ=255)

                                         ttCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGgat                                               <  1:4760607/93‑1 (MQ=255)

                                          tCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGgatg                                              <  2:4040771/93‑1 (MQ=255)

                                           cACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGgatga                                             <  2:4957988/93‑1 (MQ=255)

                                            aCCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGgatgat                                            <  1:3356652/93‑1 (MQ=255)

                                            aCCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGgatgat                                            <  1:2628500/93‑1 (MQ=255)

                                             ccGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGatgatg                                           >  2:5962761/1‑93 (MQ=255)

                                               gTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGcc                                         >  2:5851938/1‑93 (MQ=255)

                                                tCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCg                                        >  1:5465158/1‑93 (MQ=255)

                                                 cTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGt                                       >  1:6403592/1‑93 (MQ=255)

                                                  tGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTc                                      <  2:5676394/93‑1 (MQ=255)

                                                     aTCCATCGGCGAACCATCGCTGTCATTATCGGGCAGGCGCGCGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATa                                   >  1:873586/1‑93 (MQ=255)

                                                       ccATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAAt                                 <  1:6074151/93‑1 (MQ=255)

                                                         aTCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTAAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATcc                               <  1:2158362/93‑1 (MQ=255)

                                                          tCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCa                              <  1:2832345/93‑1 (MQ=255)

                                                            ggCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCAcc                            >  2:4586633/1‑93 (MQ=255)

                                                            ggCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCAcc                            <  2:1902869/93‑1 (MQ=255)

                                                            ggCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCAcc                            >  2:5994833/1‑93 (MQ=255)

                                                              cGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTg                          >  2:2351517/1‑93 (MQ=255)

                                                                aaCCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCg                        >  1:2826357/1‑93 (MQ=255)

                                                                 aCCATCGCTGTCATTATCGGCCAGGCTCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGa                       >  2:5728217/1‑93 (MQ=255)

                                                                   cATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATa                     <  1:153410/93‑1 (MQ=255)

                                                                      cGCTGTCATTATCGGCCAGTCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTg                  <  1:4455038/93‑1 (MQ=255)

                                                                        cTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCa                >  1:3160468/1‑93 (MQ=255)

                                                                         tGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCAg               >  2:3314532/1‑93 (MQ=255)

                                                                          gTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCAGa              <  2:3147104/93‑1 (MQ=255)

                                                                           tCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATATTGCAGaa             >  1:1110054/1‑93 (MQ=255)

                                                                            cATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCAGaaa            >  2:5505281/1‑93 (MQ=255)

                                                                            cATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCAGaaa            >  2:2825070/1‑93 (MQ=255)

                                                                             aTTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCAGAAAg           <  2:223327/93‑1 (MQ=255)

                                                                              ttATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCAGAAAgg          >  2:1836998/1‑93 (MQ=255)

                                                                              ttATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCAGAAAgg          <  1:2320267/93‑1 (MQ=255)

                                                                              ttATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTAATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCAGAAAgg          >  1:5244423/1‑93 (MQ=255)

                                                                               tATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCAGAAAGGc         >  2:5312321/1‑93 (MQ=255)

                                                                               tATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCAGAAAGGc         <  1:3780516/93‑1 (MQ=255)

                                                                               tATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCAGAAAGGc         <  2:5040647/93‑1 (MQ=255)

                                                                               tATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCAGAAAGGc         >  1:1308059/1‑93 (MQ=255)

                                                                               tATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCAGAAAGGc         <  1:192732/93‑1 (MQ=255)

                                                                                aTCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCAGAAAGGCt        <  2:1040324/93‑1 (MQ=255)

                                                                                 tCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCAGAAAGGCTg       <  1:3947195/93‑1 (MQ=255)

                                                                                  cGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCAGAAAGGCTgg      <  1:3326764/93‑1 (MQ=255)

                                                                                   ggCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCAGAAAGGCTGGc     <  1:5686061/93‑1 (MQ=255)

                                                                                   ggCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCAGAAAGGCTGGc     >  1:4305185/1‑93 (MQ=255)

                                                                                    gCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCAGAAAGGCTGGCg    >  1:4814637/1‑93 (MQ=255)

                                                                                      cAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCAGAAAGGCTGGCGat  <  1:5322754/93‑1 (MQ=255)

TGCAGATTGATACGCCATTCGCTGATGGAACTGGAAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGACGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCAGAAAGGCTGGCGAT  >  NC_000913/3985940‑3986118

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TTTGCAGATTGATACGCCATTCGCTGATGGAACTGGAAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGACGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCAGAAAGGCTGGCGATATCA  >  NC_000913/3985938‑3986122

TTTGCAGATTGATACGCCATTCGCTGATGGAACTGGAAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCG                                                                                             <  SRR4302534.297110/94‑1 (MQ=60)

   GCAGATTGATACGCCATTCGCTGATGGAACTGGAAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAG                                                                                          <  SRR4302534.3854015/94‑1 (MQ=60)

   GCAGATTGATACGCCATTCGCTGATGGAACTGGAAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAG                                                                                          >  SRR4302534.4900804/1‑94 (MQ=60)

    CAGATTGATACGCCATTCGCTGATGGAACTGGAAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGG                                                                                         >  SRR4302534.1009178/1‑94 (MQ=60)

    CAGATTGATACGCCATTCGCTGATGGAACTGGAAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGG                                                                                         >  SRR4302534.3440718/1‑94 (MQ=60)

    CAGATTGATACGCCATTCGCTGATGGAACTGGAAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGG                                                                                         <  SRR4302534.3711798/94‑1 (MQ=60)

    CAGATTGATACGCCATTCGCTGATGGAACTGGAAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGG                                                                                         <  SRR4302534.6417129/94‑1 (MQ=60)

    CAGATTGATACGCCATTCGCTGATGGAACTGGAAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGG                                                                                         <  SRR4302534.1301582/94‑1 (MQ=60)

    CAGATTGATACGCCATTCGCTGATGGAACTGGAAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGG                                                                                         <  SRR4302534.745866/94‑1 (MQ=60)

     AGATTGATACGCCATTCGCTGATGGAACTGGAAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGT                                                                                        >  SRR4302534.4283632/1‑94 (MQ=60)

     AGATTGATACGCCATTCGCTGATGGAACTGGAAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGT                                                                                        >  SRR4302534.1752716/1‑94 (MQ=60)

     AGATTGATACGCCATTCGCTGATGGAACTGGAAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGT                                                                                        <  SRR4302534.3885130/94‑1 (MQ=60)

      GATTGATACGCCATTCGCTGATGGAACTGGAAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTT                                                                                       <  SRR4302534.5490555/94‑1 (MQ=60)

      GATTGATACGCCATTCGCTGATGGAACTGGAAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTT                                                                                       <  SRR4302534.1706710/94‑1 (MQ=60)

      GATTGATACGCCATTCGCTGATGGAACTGGAAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTT                                                                                       <  SRR4302534.3055207/94‑1 (MQ=60)

      GATTGATACGCCATTCGCTGATGGAACTGGAAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTT                                                                                       >  SRR4302534.457466/1‑94 (MQ=60)

       ATTGATACGCCATTCGCTGATGGAACTGGAAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTA                                                                                      <  SRR4302534.1077536/94‑1 (MQ=60)

        TTGATACGCCATTCGCTGATGGAACTGGAAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTAT                                                                                     >  SRR4302534.5020434/1‑94 (MQ=60)

         TGATACGCCATTCGCTGATGGAACTGGAAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATC                                                                                    >  SRR4302534.578222/1‑94 (MQ=60)

          GATACGCCATTCGCTGATGGAACTGGAAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCT                                                                                   >  SRR4302534.6148381/1‑94 (MQ=60)

           ATACGCCATTCGCTGATGGAACTGGAAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTA                                                                                  <  SRR4302534.1264243/94‑1 (MQ=60)

           ATACGCCATTCGCTGATGGAACTGGAAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTA                                                                                  >  SRR4302534.857310/1‑94 (MQ=60)

              CGCCATTCGCTGATGGAACTGGAAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCT                                                                               <  SRR4302534.633100/94‑1 (MQ=60)

              CGCCATTCGCTGATGGAACTGGAAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCT                                                                               <  SRR4302534.236061/94‑1 (MQ=60)

               GCCATTCGCTGATGGAACTGGAAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTG                                                                              <  SRR4302534.6252880/94‑1 (MQ=60)

               GCCATTCGCTGATGGAACTGGAAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTG                                                                              <  SRR4302534.2903011/94‑1 (MQ=60)

                CCATTCGCTGATGGAACTGGAAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGA                                                                             >  SRR4302534.3162506/1‑94 (MQ=60)

                CCATTCGCTGATGGAACTGGAAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGA                                                                             >  SRR4302534.2560775/1‑94 (MQ=60)

                CCATTCGCTGATGGAACTGGAAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGA                                                                             <  SRR4302534.498136/94‑1 (MQ=60)

                CCATTCGCTGATGGAACTGGAAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGACAGGCGCAGGTTATCTACCTGA                                                                             >  SRR4302534.29437/1‑94 (MQ=60)

                 CATTCGCTGATGGAACTGGAAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGAT                                                                            >  SRR4302534.6233893/1‑94 (MQ=60)

                 CATTCGCTGATGGAACTGGAAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGAT                                                                            >  SRR4302534.4984821/1‑94 (MQ=60)

                 CATTCGCTGATGGAACTGGAAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGAT                                                                            >  SRR4302534.623787/1‑94 (MQ=60)

                 CATTCGCTGATGGAACTGGAAAGCTCTTCACAGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGTCAGGCGCAGGATATCTACCTGAT                                                                            >  SRR4302534.4281051/1‑94 (MQ=60)

                  ATTCGCTGATGGAACTGGAAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATT                                                                           >  SRR4302534.4778623/1‑94 (MQ=60)

                        TGATGGAACTGGAAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAG                                                                     >  SRR4302534.6455338/1‑94 (MQ=60)

                        TGATGGAACTGGAAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAG                                                                     >  SRR4302534.3545384/1‑94 (MQ=60)

                          ATGGAACTGGAAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCT                                                                   <  SRR4302534.5100110/94‑1 (MQ=60)

                           TGGAACTGGAAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTG                                                                  <  SRR4302534.2524127/94‑1 (MQ=60)

                           TGGAACTGGAAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTG                                                                  <  SRR4302534.4207663/94‑1 (MQ=60)

                           TGGAACTGGAAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTG                                                                  >  SRR4302534.3037982/1‑94 (MQ=60)

                            GGAACTGGAAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGA                                                                 >  SRR4302534.4084769/1‑94 (MQ=60)

                             GAACTGGAAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGAT                                                                >  SRR4302534.620418/1‑94 (MQ=60)

                             GAACTGGAAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGAT                                                                <  SRR4302534.605092/94‑1 (MQ=60)

                              AACTGGAAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATT                                                               <  SRR4302534.4479012/94‑1 (MQ=60)

                              AACTGGAAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATT                                                               <  SRR4302534.1397759/94‑1 (MQ=60)

                               ACTGGAAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTA                                                              >  SRR4302534.4373081/1‑94 (MQ=60)

                               ACTGGAAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTA                                                              >  SRR4302534.1371320/1‑94 (MQ=60)

                                CTGGAAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAA                                                             >  SRR4302534.5867915/1‑94 (MQ=60)

                                 TGGAAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAG                                                            >  SRR4302534.6641615/1‑94 (MQ=60)

                                   GAAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCT                                                          <  SRR4302534.2572919/94‑1 (MQ=60)

                                    AAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTT                                                         >  SRR4302534.3926418/1‑94 (MQ=60)

                                    AAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTT                                                         >  SRR4302534.155088/1‑94 (MQ=60)

                                    AAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTT                                                         <  SRR4302534.817351/94‑1 (MQ=60)

                                    AAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTT                                                         >  SRR4302534.1880925/1‑94 (MQ=60)

                                     AAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTA                                                        >  SRR4302534.1026367/1‑94 (MQ=60)

                                     AAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTA                                                        <  SRR4302534.487585/94‑1 (MQ=60)

                                     AAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTA                                                        >  SRR4302534.3987005/1‑94 (MQ=60)

                                     AAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTA                                                        >  SRR4302534.3931569/1‑94 (MQ=60)

                                      AGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAA                                                       <  SRR4302534.247787/94‑1 (MQ=60)

                                         TCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGA                                                    <  SRR4302534.1179616/94‑1 (MQ=60)

                                         TCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGA                                                    >  SRR4302534.2838074/1‑94 (MQ=60)

                                         TCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGA                                                    <  SRR4302534.5528693/94‑1 (MQ=60)

                                          CTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGAT                                                   <  SRR4302534.912830/94‑1 (MQ=60)

                                          CGTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGAT                                                   <  SRR4302534.6312057/94‑1 (MQ=60)

                                           TTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATG                                                  <  SRR4302534.4889873/94‑1 (MQ=60)

                                            TCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGA                                                 >  SRR4302534.3639573/1‑94 (MQ=60)

                                             CACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGAT                                                >  SRR4302534.2185200/1‑94 (MQ=60)

                                             CACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGAT                                                >  SRR4302534.2432121/1‑94 (MQ=60)

                                              ACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATG                                               <  SRR4302534.3446550/94‑1 (MQ=60)

                                              ACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATG                                               <  SRR4302534.2698031/94‑1 (MQ=60)

                                                 GTCTGAATCCATCGGCGAGCCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCG                                            <  SRR4302534.2899410/94‑1 (MQ=60)

                                                 GTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCG                                            <  SRR4302534.3548680/94‑1 (MQ=60)

                                                 GTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCG                                            >  SRR4302534.5612472/1‑94 (MQ=60)

                                                 GTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGGTATCTACCTGATTTGAAAGCTGCTTAAGCTTAAGGATGATGCCG                                            >  SRR4302534.4690899/1‑94 (MQ=60)

                                                  TCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGT                                           <  SRR4302534.3911405/94‑1 (MQ=60)

                                                  TCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGT                                           >  SRR4302534.1369989/1‑94 (MQ=60)

                                                  TCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGT                                           >  SRR4302534.6576465/1‑94 (MQ=60)

                                                   CTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTC                                          <  SRR4302534.5021733/94‑1 (MQ=60)

                                                   CTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTC                                          >  SRR4302534.2692477/1‑94 (MQ=60)

                                                     GAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCAT                                        <  SRR4302534.991186/94‑1 (MQ=60)

                                                      AATCCATCGGCGAACCATCGCTGTCATTATCGGGCAGGCGCGCGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATA                                       >  SRR4302534.895672/1‑94 (MQ=60)

                                                         CCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATC                                    >  SRR4302534.3005583/1‑94 (MQ=60)

                                                         CCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATC                                    <  SRR4302534.1954027/94‑1 (MQ=60)

                                                         CCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATC                                    <  SRR4302534.6239307/94‑1 (MQ=60)

                                                         CCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATC                                    >  SRR4302534.3286765/1‑94 (MQ=60)

                                                         CCATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATC                                    >  SRR4302534.6117752/1‑94 (MQ=60)

                                                           ATCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTAAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCA                                  <  SRR4302534.2215342/94‑1 (MQ=60)

                                                            TCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCAC                                 <  SRR4302534.2906773/94‑1 (MQ=60)

                                                            TCGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCAC                                 >  SRR4302534.4042193/1‑94 (MQ=60)

                                                             CGGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACC                                >  SRR4302534.2469679/1‑94 (MQ=60)

                                                              GGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCT                               >  SRR4302534.4426869/1‑94 (MQ=60)

                                                              GGCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCT                               <  SRR4302534.2846169/94‑1 (MQ=60)

                                                               GCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTG                              <  SRR4302534.214374/94‑1 (MQ=60)

                                                               GCGAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTG                              <  SRR4302534.5918462/94‑1 (MQ=60)

                                                                 GAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCG                            >  SRR4302534.2727391/1‑94 (MQ=60)

                                                                 GAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCG                            >  SRR4302534.2900651/1‑94 (MQ=60)

                                                                 GAACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCG                            >  SRR4302534.4570579/1‑94 (MQ=60)

                                                                  AGCCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATTCCGTCATAATTCACCTGCGA                           <  SRR4302534.6464397/94‑1 (MQ=60)

                                                                  AACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGA                           >  SRR4302534.1861936/1‑94 (MQ=60)

                                                                  AACCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGA                           >  SRR4302534.1331144/1‑94 (MQ=60)

                                                                    CCATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATA                         <  SRR4302534.5856430/94‑1 (MQ=60)

                                                                     CATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATAC                        <  SRR4302534.157407/94‑1 (MQ=60)

                                                                     CATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATAC                        >  SRR4302534.5858777/1‑94 (MQ=60)

                                                                      ATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACT                       <  SRR4302534.1742783/94‑1 (MQ=60)

                                                                      ATCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACT                       <  SRR4302534.3529562/94‑1 (MQ=60)

                                                                       TCGCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTG                      >  SRR4302534.1948738/1‑94 (MQ=60)

                                                                        CGCTGTCATTATCGGCCAGTCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGC                     <  SRR4302534.4575460/94‑1 (MQ=60)

                                                                         GCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCA                    <  SRR4302534.3433009/94‑1 (MQ=60)

                                                                         GCTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCA                    >  SRR4302534.3243744/1‑94 (MQ=60)

                                                                          CTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCAG                   <  SRR4302534.4445854/94‑1 (MQ=60)

                                                                          CTGTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCAG                   <  SRR4302534.489589/94‑1 (MQ=60)

                                                                            GTCATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATATTGCAGAA                 >  SRR4302534.1139006/1‑94 (MQ=60)

                                                                               ATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCGCCTGCGATACTGCAGAAAGG              <  SRR4302534.6435684/94‑1 (MQ=60)

                                                                               ATTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTAATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCAGAAAGG              >  SRR4302534.5386077/1‑94 (MQ=60)

                                                                                TTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCAGAAAGGC             >  SRR4302534.1342569/1‑94 (MQ=60)

                                                                                TTATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCAGAAAGGC             <  SRR4302534.2381842/94‑1 (MQ=60)

                                                                                 TATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCAGAAAGGCT            <  SRR4302534.607851/94‑1 (MQ=60)

                                                                                 TATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCAGAAAGGCT            <  SRR4302534.197774/94‑1 (MQ=60)

                                                                                 TATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCAGAAAGGCT            <  SRR4302534.3882021/94‑1 (MQ=60)

                                                                                  ATCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCAGAAAGGCTG           >  SRR4302534.5431977/1‑94 (MQ=60)

                                                                                   TCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTTCGATACTGCAGAAAGGCTGG          <  SRR4302534.6470986/94‑1 (MQ=60)

                                                                                   TCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCAGAAAGGCTGG          <  SRR4302534.3941836/94‑1 (MQ=60)

                                                                                   TCGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCAGAAAGGCTGG          <  SRR4302534.4052875/94‑1 (MQ=60)

                                                                                    CGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCAGAAAGGCTGGC         <  SRR4302534.3415732/94‑1 (MQ=60)

                                                                                    CGGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCAGAAAGGCTGGC         >  SRR4302534.4421885/1‑94 (MQ=60)

                                                                                    CGGCCAGGCGCAGGGTATTTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCAGAAAGGCTGGC         <  SRR4302534.5192712/94‑1 (MQ=60)

                                                                                     GGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCAGAAAGGCTGGCG        >  SRR4302534.4945333/1‑94 (MQ=60)

                                                                                     GGCCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCAGAAAGGCTGGCG        <  SRR4302534.5840045/94‑1 (MQ=60)

                                                                                       CCAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCAGAAAGGCTGGCGAT      >  SRR4302534.5166318/1‑94 (MQ=60)

                                                                                        CAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCAGAAAGGCTGGCGATA     <  SRR4302534.5466312/94‑1 (MQ=60)

                                                                                        CAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCAGAAAGGCTGGCGATA     >  SRR4302534.1179967/1‑94 (MQ=60)

                                                                                        CAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCAGAAAGGCTGGCGATA     >  SRR4302534.1504113/1‑94 (MQ=60)

                                                                                        CAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCAGAAAGGCTGGCGATA     >  SRR4302534.4284390/1‑94 (MQ=60)

                                                                                        CAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCAGAAAGGCTGGCGATA     >  SRR4302534.6267481/1‑94 (MQ=60)

                                                                                        CAGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCAGAAAGGCTGGCGATA     >  SRR4302534.5827682/1‑94 (MQ=60)

                                                                                         AGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCAGAAAGGCTGGCGATAT    >  SRR4302534.6040995/1‑94 (MQ=60)

                                                                                         AGGCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCAGAAAGGCTGGCGATAT    >  SRR4302534.5217041/1‑94 (MQ=60)

                                                                                           GCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCAGAAAGGCTGGCGATATCA  <  SRR4302534.10084/94‑1 (MQ=60)

                                                                                           GCGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGAAGCCGTCATAATCCACCTGCGATACTGCAGAAAGGCTGGCGATATCA  >  SRR4302534.5322119/1‑94 (MQ=60)

TTTGCAGATTGATACGCCATTCGCTGATGGAACTGGAAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGACGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCAGAAAGGCTGGCGATATCA  >  NC_000913/3985938‑3986122

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`