Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	884	257.2	12958910	96.9%	12557183	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,001,717	+C	coding (86/903 nt)	rarD ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,001,717	1	.	C	100.0%	701.2 / NA	208	T29R (ACG→AGG)	rarD	hypothetical protein
Reads supporting (aligned to +/- strand): ref base . (0/0); new base C (89/119); total (89/119)

Only 100 of 237 total aligned reads displayed.

TGCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACG‑TGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACTCATT  >  NC_000913/4001625‑4001803

tGCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACg                                                                                         <  2:769867/93‑1 (MQ=255)

 gCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACgc                                                                                        >  2:3719086/1‑92 (MQ=255)

  cTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACgct                                                                                       <  1:2334993/93‑3 (MQ=255)

   tcatcaGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTTAAGTACgctg                                                                                      >  1:5571483/1‑90 (MQ=255)

   tcatcaGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGCAGTACgctg                                                                                      >  1:2697908/1‑90 (MQ=255)

   tcatcaGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAAATTGAAGTACgctg                                                                                      >  1:4502285/1‑90 (MQ=255)

    catcaGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTTAAGTACgctgg                                                                                     >  1:2244818/1‑89 (MQ=255)

       caGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGgcgc                                                                                  <  1:668225/93‑1 (MQ=255)

           accaccATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCtata                                                                              >  2:1529801/1‑93 (MQ=255)

            ccaccaTAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATAc                                                                             <  1:4074664/93‑1 (MQ=255)

             caccaTAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATAcc                                                                            >  1:2511515/1‑93 (MQ=255)

             caccaTAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGCAGTAAATCAACTTGAAGTACGCTGGCGCTATAcc                                                                            >  2:3280661/1‑93 (MQ=255)

              accaTAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAAATTGAAGTACGCTGGCGCTATAccc                                                                           >  1:5315777/1‑93 (MQ=255)

              accaTAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGGAGTAAATAAACTTGAAGTACGCTGGCGCTATAccc                                                                           >  2:1130891/1‑93 (MQ=255)

               ccaTAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATAcccc                                                                          <  2:4581840/93‑1 (MQ=255)

                 aTAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTTAAGTACGCTTGCGCTATAAACCaa                                                                        >  1:3986185/1‑93 (MQ=255)

                 aTAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCaa                                                                        <  2:1782113/93‑1 (MQ=255)

                   aaaGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCATGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCaaat                                                                      <  2:4521770/93‑1 (MQ=255)

                   aaaGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCaaat                                                                      >  2:4649552/1‑93 (MQ=255)

                    aaGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATTAAATTTAAGGACGCTGGCGCTATACCCCcaata                                                                     >  1:1292443/1‑93 (MQ=255)

                    aaGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCaaata                                                                     >  1:4227844/1‑93 (MQ=255)

                     aGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCaaataa                                                                    >  1:1852290/1‑93 (MQ=255)

                     aGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCaaataa                                                                    >  2:1238685/1‑93 (MQ=255)

                     aGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCaaataa                                                                    >  2:3637254/1‑93 (MQ=255)

                         aaaaGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCaaataaaata                                                                >  1:4332770/1‑93 (MQ=255)

                         aaaCGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCaaataaaata                                                                >  2:5313152/1‑93 (MQ=255)

                          aaCGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCCaataaaataa                                                               >  1:1109869/1‑93 (MQ=255)

                            cGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGc                                                             >  2:2760008/1‑93 (MQ=255)

                             gACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTTGCGCTATACCCCAAATAAAATAAGCg                                                            >  2:235718/1‑93 (MQ=255)

                             gACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCg                                                            <  2:2821078/93‑1 (MQ=255)

                             gACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCg                                                            <  2:6202890/93‑1 (MQ=255)

                              aCCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGGAAGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCgg                                                           >  1:2866687/1‑93 (MQ=255)

                              aCCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCgg                                                           >  1:2935924/1‑93 (MQ=255)

                                cAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCggga                                                         >  1:2130998/1‑91 (MQ=255)

                                 aGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCaa                                                        <  2:2493796/93‑1 (MQ=255)

                                 aGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCaa                                                        <  1:344248/93‑1 (MQ=255)

                                 aGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGGAGTAAATCAACTTGAAGTAGGCTGGCGCTATACCCCCAATAAAATAAGAGACaa                                                        >  2:2895775/1‑93 (MQ=255)

                                  gATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAag                                                       >  2:2768634/1‑93 (MQ=255)

                                   aTCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTTGCGCTATACCCCAAATAAAATAAGCCGCAaga                                                      >  2:6423293/1‑93 (MQ=255)

                                   aTCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAaga                                                      >  2:5902706/1‑93 (MQ=255)

                                   aTCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAaga                                                      <  2:1318858/93‑1 (MQ=255)

                                   aTCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAaga                                                      <  1:3546099/93‑1 (MQ=255)

                                    tCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAagag                                                     >  2:5647640/1‑93 (MQ=255)

                                     cACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGGAagagg                                                    >  1:1664362/1‑92 (MQ=255)

                                     cACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGc                                                    >  2:1085492/1‑93 (MQ=255)

                                     cACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGc                                                    <  1:2801830/93‑1 (MQ=255)

                                       cgcgATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGa                                                  <  2:5695640/93‑1 (MQ=255)

                                       cgcgATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGa                                                  <  1:6086783/93‑1 (MQ=255)

                                        gcgATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAg                                                 <  1:791580/93‑1 (MQ=255)

                                         cgATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGt                                                >  2:1758716/1‑93 (MQ=255)

                                          gATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAgga                                               >  1:5621412/1‑91 (MQ=255)

                                            tGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAAt                                             >  2:297208/1‑93 (MQ=255)

                                             gCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATa                                            <  1:6113743/93‑1 (MQ=255)

                                             gCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATa                                            <  1:889215/93‑1 (MQ=255)

                                               gTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTTAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATaag                                          >  1:3811734/1‑91 (MQ=255)

                                               gTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACg                                          >  2:3273268/1‑93 (MQ=255)

                                                  aGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGccc                                       <  2:2063268/93‑1 (MQ=255)

                                                  aGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGccc                                       <  1:4687865/93‑1 (MQ=255)

                                                  aGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGccc                                       <  2:1404864/93‑1 (MQ=255)

                                                   ggATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCt                                      <  2:3391906/93‑1 (MQ=255)

                                                   ggATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCt                                      <  2:3924470/93‑1 (MQ=255)

                                                   ggATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAATAATACGCCCt                                      >  1:573964/1‑93 (MQ=255)

                                                     aTTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGc                                    <  1:107507/93‑1 (MQ=255)

                                                         cATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCcgcg                                >  1:841393/1‑93 (MQ=255)

                                                          aTCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATCAGCGGCAAGAGCGAGTAATACGCCCTGCCGCgt                               <  2:6305359/93‑1 (MQ=255)

                                                          aTCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCgt                               <  1:5813957/93‑1 (MQ=255)

                                                           tCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCgtt                              <  1:1582714/93‑1 (MQ=255)

                                                           tCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCgtt                              >  2:4784692/1‑93 (MQ=255)

                                                           tCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCgtt                              <  1:5469392/93‑1 (MQ=255)

                                                             ggcggGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCgtttg                            <  1:5136956/93‑1 (MQ=255)

                                                               cggGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCgtttgtt                          <  1:706429/93‑1 (MQ=255)

                                                               cggGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCgtttgtt                          <  1:1006902/93‑1 (MQ=255)

                                                                gggCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCgtttgttt                         <  2:1294392/93‑1 (MQ=255)

                                                                 ggCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATCCCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGtttt                        <  2:1868041/93‑1 (MQ=255)

                                                                    aCGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGca                     <  1:4826529/93‑1 (MQ=255)

                                                                    aCGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGca                     >  1:2099285/1‑93 (MQ=255)

                                                                    aCGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGca                     >  2:5126704/1‑93 (MQ=255)

                                                                    aCGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGca                     >  1:4908919/1‑93 (MQ=255)

                                                                    aCGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGca                     >  1:5259007/1‑93 (MQ=255)

                                                                     cGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGcat                    >  1:3239725/1‑93 (MQ=255)

                                                                       tagtaAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGcatcc                  <  1:241343/93‑1 (MQ=255)

                                                                         gtaAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGcatccat                <  1:1327457/93‑1 (MQ=255)

                                                                         gtaAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGcatccat                >  1:4565536/1‑93 (MQ=255)

                                                                         gtaAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGcatccat                <  2:4194247/93‑1 (MQ=255)

                                                                         gtaAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGcatccat                >  2:1967580/1‑93 (MQ=255)

                                                                          taAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGcatccatc               >  1:1248442/1‑93 (MQ=255)

                                                                          taAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGcatccatc               <  1:62217/93‑1 (MQ=255)

                                                                           aaaTCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCg              <  1:2540025/93‑1 (MQ=255)

                                                                           aaaTCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCg              >  1:1977997/1‑93 (MQ=255)

                                                                             aTCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCggg            <  2:4981296/93‑1 (MQ=255)

                                                                                aaCTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAAt         <  2:1435859/93‑1 (MQ=255)

                                                                                aaCTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAAt         <  1:239913/93‑1 (MQ=255)

                                                                                aaCTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAAt         <  2:6298092/93‑1 (MQ=255)

                                                                                  cTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATAc       >  2:5396568/1‑93 (MQ=255)

                                                                                   ttGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACt      <  1:5303266/93‑1 (MQ=255)

                                                                                    tGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACTc     >  1:4645502/1‑93 (MQ=255)

                                                                                     gAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACTCa    <  2:2583004/93‑1 (MQ=255)

                                                                                     gAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACTCa    <  2:3128251/93‑1 (MQ=255)

                                                                                     gAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACTCa    >  1:1909459/1‑93 (MQ=255)

                                                                                       aGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACTCAtt  >  1:6426501/1‑93 (MQ=255)

TGCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACG‑TGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACTCATT  >  NC_000913/4001625‑4001803

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACG‑TGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACTCATTTTTAATT  >  NC_000913/4001626‑4001810

GCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGct                                                                                              <  SRR4302534.1070716/94‑3 (MQ=60)

 CTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTTAAGTACGctg                                                                                             >  SRR4302534.5721934/1‑91 (MQ=60)

 CTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGCAGTACGctg                                                                                             >  SRR4302534.2769183/1‑91 (MQ=60)

 CTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGctg                                                                                             >  SRR4302534.1106367/1‑91 (MQ=60)

 CTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGctg                                                                                             <  SRR4302534.2396934/94‑4 (MQ=60)

 CTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGctg                                                                                             <  SRR4302534.5613394/94‑4 (MQ=60)

 CTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAAATTGAAGTACGctg                                                                                             >  SRR4302534.4624147/1‑91 (MQ=60)

  TCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTTAAGTACGCTGG                                                                                            >  SRR4302534.2304315/1‑94 (MQ=60)

   CATCAGCACCACCATAAAGAAAAGCGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGC                                                                                           <  SRR4302534.2905044/94‑1 (MQ=60)

    ATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAAACAACTTGAAGGACGCTGGCG                                                                                          <  SRR4302534.6489421/94‑1 (MQ=60)

     TCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGC                                                                                         <  SRR4302534.3548590/94‑1 (MQ=60)

      CAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCT                                                                                        <  SRR4302534.712429/94‑1 (MQ=60)

      CAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCT                                                                                        <  SRR4302534.684505/94‑1 (MQ=60)

       AGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTTGCGCTA                                                                                       >  SRR4302534.4968542/1‑94 (MQ=60)

          CCCACCACAATGAAAACCGACCAGATCACGCGAGCCGTCAGCATTTCATCGGCGCGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATAC                                                                                    <  SRR4302534.861082/94‑1 (MQ=55)

           CCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACC                                                                                   <  SRR4302534.4184019/94‑1 (MQ=60)

           CCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACC                                                                                   >  SRR4302534.2578195/1‑94 (MQ=60)

           CCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTCGCGCTATACC                                                                                   >  SRR4302534.2096495/1‑94 (MQ=60)

            CACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAAATTGAAGTACGCTGGCGCTATACCC                                                                                  >  SRR4302534.5459179/1‑94 (MQ=60)

             ACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCC                                                                                 <  SRR4302534.1277677/94‑1 (MQ=60)

              CCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCA                                                                                >  SRR4302534.4240614/1‑94 (MQ=60)

               CATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTTAAGTACGcttgcgctataaaccaa                                                                               >  SRR4302534.4092846/1‑77 (MQ=60)

               CATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAA                                                                               >  SRR4302534.2262064/1‑94 (MQ=60)

                  AAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATTAAATTTAAGGACGCTGGCGCTATACCCCCAATA                                                                            >  SRR4302534.1326478/1‑94 (MQ=60)

                  AAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATA                                                                            >  SRR4302534.4341778/1‑94 (MQ=60)

                   AAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAA                                                                           >  SRR4302534.1900476/1‑94 (MQ=60)

                    AGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAA                                                                          >  SRR4302534.3614002/1‑94 (MQ=60)

                      AAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAAT                                                                        >  SRR4302534.1327546/1‑94 (MQ=60)

                       AAAAAGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATA                                                                       >  SRR4302534.4449920/1‑94 (MQ=60)

                        AAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCCAATAAAATAA                                                                      >  SRR4302534.1138816/1‑94 (MQ=60)

                        AAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAA                                                                      >  SRR4302534.5395598/1‑94 (MQ=60)

                        AAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAA                                                                      <  SRR4302534.6265314/94‑1 (MQ=60)

                         AACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAG                                                                     <  SRR4302534.5821979/94‑1 (MQ=60)

                          ACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGC                                                                    <  SRR4302534.4720024/94‑1 (MQ=60)

                          ACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGC                                                                    <  SRR4302534.2578408/94‑1 (MQ=60)

                           CTACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCG                                                                   <  SRR4302534.1844633/94‑1 (MQ=60)

                           CGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCG                                                                   <  SRR4302534.2159511/94‑1 (MQ=60)

                            GACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGGAAGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGG                                                                  >  SRR4302534.2941969/1‑94 (MQ=60)

                            GACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGG                                                                  <  SRR4302534.6175599/94‑1 (MQ=60)

                            GACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGG                                                                  >  SRR4302534.3012980/1‑94 (MQ=60)

                            GACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAACGG                                                                  >  SRR4302534.5714479/1‑94 (MQ=60)

                              CCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGGA                                                                >  SRR4302534.2187134/1‑94 (MQ=60)

                              CCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCA                                                                <  SRR4302534.3217769/94‑1 (MQ=60)

                               CAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAA                                                               <  SRR4302534.1616452/94‑1 (MQ=60)

                                AGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAG                                                              <  SRR4302534.353064/94‑1 (MQ=60)

                                AGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAG                                                              <  SRR4302534.1969097/94‑1 (MQ=60)

                                AGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAG                                                              >  SRR4302534.116948/1‑94 (MQ=60)

                                AGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAACGGCAAG                                                              >  SRR4302534.132557/1‑94 (MQ=60)

                                  ATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCCACTTGAAGTACGCTGGGGCTATACCCCCAATAAAATAAACGGCAAGAG                                                            >  SRR4302534.90713/1‑94 (MQ=60)

                                  ATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGGGCTATACCCCAAATAAAATAAGCGGCAAGAG                                                            <  SRR4302534.838468/94‑1 (MQ=60)

                                  ATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAG                                                            <  SRR4302534.3641474/94‑1 (MQ=60)

                                  ATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAG                                                            <  SRR4302534.5060624/94‑1 (MQ=60)

                                   TCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTTAAGTACGCTGGCGCTATACCCCAAATAAAATAAACGGCAAGAGC                                                           >  SRR4302534.5567966/1‑94 (MQ=60)

                                   TCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGGAAGAGG                                                           >  SRR4302534.1707686/1‑94 (MQ=60)

                                    CACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCG                                                          <  SRR4302534.2875512/94‑1 (MQ=60)

                                    CACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCG                                                          <  SRR4302534.4435980/94‑1 (MQ=60)

                                      CGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAG                                                        <  SRR4302534.881419/94‑1 (MQ=60)

                                      CGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAG                                                        <  SRR4302534.6252290/94‑1 (MQ=60)

                                       GCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGT                                                       <  SRR4302534.3528369/94‑1 (MQ=60)

                                       GCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGT                                                       <  SRR4302534.4524448/94‑1 (MQ=60)

                                       GCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGT                                                       <  SRR4302534.811406/94‑1 (MQ=60)

                                        CGATGCTGCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTA                                                      <  SRR4302534.2790296/94‑1 (MQ=60)

                                        CGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTA                                                      <  SRR4302534.1657920/94‑1 (MQ=60)

                                        CGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGGA                                                      >  SRR4302534.5773527/1‑94 (MQ=60)

                                        CGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGAGAGGA                                                      >  SRR4302534.261941/1‑94 (MQ=60)

                                         GATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAA                                                     >  SRR4302534.3003709/1‑94 (MQ=60)

                                            GCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATAC                                                  <  SRR4302534.911731/94‑1 (MQ=60)

                                            GCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATAC                                                  <  SRR4302534.6280072/94‑1 (MQ=60)

                                             CGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTTAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATAAG                                                 >  SRR4302534.3914093/1‑94 (MQ=60)

                                             CGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACG                                                 <  SRR4302534.6288775/94‑1 (MQ=60)

                                                 AGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCT                                             <  SRR4302534.400519/94‑1 (MQ=60)

                                                 AGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCT                                             <  SRR4302534.4815110/94‑1 (MQ=60)

                                                 AGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCT                                             >  SRR4302534.747008/1‑94 (MQ=60)

                                                 AGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCT                                             >  SRR4302534.4642926/1‑94 (MQ=60)

                                                 AGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCT                                             >  SRR4302534.1928830/1‑94 (MQ=60)

                                                 AGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAATAATACGCCCT                                             >  SRR4302534.588049/1‑94 (MQ=60)

                                                    ATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCC                                          <  SRR4302534.110378/94‑1 (MQ=60)

                                                       TCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCG                                       >  SRR4302534.862587/1‑94 (MQ=60)

                                                         ATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTT                                     <  SRR4302534.4158715/94‑1 (MQ=60)

                                                         ATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTT                                     <  SRR4302534.5971757/94‑1 (MQ=60)

                                                          TCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTT                                    <  SRR4302534.5616822/94‑1 (MQ=60)

                                                          TCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTT                                    <  SRR4302534.960849/94‑1 (MQ=60)

                                                          TCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTT                                    <  SRR4302534.1624110/94‑1 (MQ=60)

                                                          TCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTT                                    <  SRR4302534.1931958/94‑1 (MQ=60)

                                                          TCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTT                                    <  SRR4302534.5408784/94‑1 (MQ=60)

                                                           CGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTG                                   >  SRR4302534.96536/1‑94 (MQ=60)

                                                            GGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGT                                  <  SRR4302534.5275610/94‑1 (MQ=60)

                                                              CGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTT                                <  SRR4302534.1032734/94‑1 (MQ=60)

                                                              CGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTT                                <  SRR4302534.723767/94‑1 (MQ=60)

                                                              CGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTT                                <  SRR4302534.2371300/94‑1 (MQ=60)

                                                                  CACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCA                            >  SRR4302534.5401108/1‑94 (MQ=60)

                                                                  CACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCA                            >  SRR4302534.2154376/1‑94 (MQ=60)

                                                                  CACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCA                            >  SRR4302534.5041973/1‑94 (MQ=60)

                                                                   ACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCAT                           >  SRR4302534.2109589/1‑94 (MQ=60)

                                                                   ACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCAT                           >  SRR4302534.3325918/1‑94 (MQ=60)

                                                                   ACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCAT                           <  SRR4302534.6153292/94‑1 (MQ=60)

                                                                   ACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCAT                           <  SRR4302534.4957504/94‑1 (MQ=60)

                                                                   ACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCAT                           <  SRR4302534.1470050/94‑1 (MQ=60)

                                                                     GTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCC                         <  SRR4302534.2552057/94‑1 (MQ=60)

                                                                     GTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCC                         >  SRR4302534.5746132/1‑94 (MQ=60)

                                                                      TAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCA                        <  SRR4302534.247659/94‑1 (MQ=60)

                                                                      TAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCA                        <  SRR4302534.371226/94‑1 (MQ=60)

                                                                      TAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCA                        <  SRR4302534.6531830/94‑1 (MQ=60)

                                                                      TAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCA                        <  SRR4302534.4112784/94‑1 (MQ=60)

                                                                      TAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTGTTGCATCCA                        <  SRR4302534.6494953/94‑1 (MQ=60)

                                                                       AGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGTATCCAT                       >  SRR4302534.5334355/1‑94 (MQ=60)

                                                                       AGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCAT                       <  SRR4302534.3005786/94‑1 (MQ=60)

                                                                       AGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCAT                       <  SRR4302534.72956/94‑1 (MQ=60)

                                                                       AGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCAT                       >  SRR4302534.4689309/1‑94 (MQ=60)

                                                                        GTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATC                      >  SRR4302534.1281347/1‑94 (MQ=60)

                                                                        GTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATC                      <  SRR4302534.2465801/94‑1 (MQ=60)

                                                                        GTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATC                      <  SRR4302534.1362453/94‑1 (MQ=60)

                                                                         TAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCG                     >  SRR4302534.2029783/1‑94 (MQ=60)

                                                                         TAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCG                     >  SRR4302534.4845695/1‑94 (MQ=60)

                                                                         TAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCG                     <  SRR4302534.63915/94‑1 (MQ=60)

                                                                         GAAATCAACTTGAAGTACGCTGGCTCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCG                     <  SRR4302534.2758730/94‑1 (MQ=60)

                                                                          AAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGG                    <  SRR4302534.2607416/94‑1 (MQ=60)

                                                                           AATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGG                   <  SRR4302534.5856190/94‑1 (MQ=60)

                                                                           AATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGG                   <  SRR4302534.244068/94‑1 (MQ=60)

                                                                            ATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGA                  <  SRR4302534.2561669/94‑1 (MQ=60)

                                                                             TCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAA                 >  SRR4302534.6615706/1‑94 (MQ=60)

                                                                              CAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAAT                <  SRR4302534.4733606/94‑1 (MQ=60)

                                                                               AACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATA               <  SRR4302534.246194/94‑1 (MQ=60)

                                                                               AACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATA               >  SRR4302534.4383602/1‑94 (MQ=60)

                                                                               AACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGCTTGTTTTtttttcctcctgtaaa               >  SRR4302534.5875394/1‑78 (MQ=60)

                                                                                ACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATAC              <  SRR4302534.5917414/94‑1 (MQ=60)

                                                                                ACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATAC              >  SRR4302534.5454704/1‑94 (MQ=60)

                                                                                 CTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACT             >  SRR4302534.2314281/1‑94 (MQ=60)

                                                                                 CTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACT             <  SRR4302534.4973254/94‑1 (MQ=60)

                                                                                  TTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACTC            >  SRR4302534.4771607/1‑94 (MQ=60)

                                                                                  TTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACTC            <  SRR4302534.5446359/94‑1 (MQ=60)

                                                                                   TGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACTCA           >  SRR4302534.1959293/1‑94 (MQ=60)

                                                                                     AAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACTCATT         >  SRR4302534.6599811/1‑94 (MQ=60)

                                                                                     AAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACTCATT         >  SRR4302534.479039/1‑94 (MQ=60)

                                                                                       GTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACTCATTTT       <  SRR4302534.3845885/94‑1 (MQ=60)

                                                                                       GTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTCTGTTTTGCATCCATCGGGAATACTCATTTT       >  SRR4302534.5267339/1‑94 (MQ=60)

                                                                                        TACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACTCATTTTT      <  SRR4302534.2262171/94‑1 (MQ=60)

                                                                                        TACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACTCATTTTT      >  SRR4302534.499267/1‑94 (MQ=60)

                                                                                        TACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGCAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACTCATTTTT      <  SRR4302534.6251830/94‑1 (MQ=60)

                                                                                           C‑TGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACTCATTTTTAATT  <  SRR4302534.6269202/94‑1 (MQ=60)

                                                                                           C‑TGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACTCATTTTTAATT  <  SRR4302534.5586551/94‑1 (MQ=60)

GCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACG‑TGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACTCATTTTTAATT  >  NC_000913/4001626‑4001810

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`