Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 884 257.2 12958910 96.9% 12557183 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,006,483 +C coding (183/207 nt) b4554 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,006,4801.C98.9% 602.6 / ‑0.7 176coding (186/207 nt)b4554hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (1/1);  new base C (96/78);  total (97/79)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.36e-01

Only 100 of 199 total aligned reads displayed.

GGCGCGCCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGT‑CCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGG  >  NC_000913/4006388‑4006567
                                                                                             |                                                                                       
 gcgcgcCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGT‑c                                                                                        <  1:2159984/93‑1 (MQ=255)
 gcgcgcCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGT‑c                                                                                        >  1:2838416/1‑93 (MQ=255)
  cgcgcCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGT‑cc                                                                                       >  1:1686913/1‑93 (MQ=255)
  cgcgcCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGT‑cc                                                                                       >  1:4028546/1‑93 (MQ=255)
    cgcCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGT‑cccc                                                                                     <  1:4949246/93‑2 (MQ=255)
    cgcCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGT‑cccc                                                                                     >  1:97979/1‑92 (MQ=255)
     gcCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGT‑cccca                                                                                    >  1:79009/1‑91 (MQ=255)
     gcCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGT‑cccca                                                                                    >  1:3495517/1‑91 (MQ=255)
      ccACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGT‑ccccaa                                                                                   <  1:5509350/93‑4 (MQ=255)
      ccACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGT‑ccccaa                                                                                   <  1:6435612/93‑4 (MQ=255)
      ccACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGT‑ccccaa                                                                                   <  2:1390017/93‑4 (MQ=255)
       cACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGT‑ccccaac                                                                                  >  2:1345327/1‑89 (MQ=255)
         ctgctgGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGT‑CCCCAaccc                                                                                >  2:3030605/1‑93 (MQ=255)
          tgctgGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAAcccc                                                                                >  1:5928209/1‑93 (MQ=255)
          tgctgGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAAcccc                                                                                <  1:5121029/93‑1 (MQ=255)
          tgctgGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAAcccc                                                                                >  1:877006/1‑93 (MQ=255)
            ctgGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCAc                                                                              <  2:5319759/93‑1 (MQ=255)
              ggATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGc                                                                            >  1:817692/1‑93 (MQ=255)
                 ttCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCaa                                                                         >  1:222744/1‑93 (MQ=255)
                 ttCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTAAACACTTCAAACGCAATCACTGAGCGGGAAACTAGCGTCCCCAACCCCACGCCaa                                                                         >  1:2046955/1‑93 (MQ=255)
                  tCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAAc                                                                        >  1:1807985/1‑93 (MQ=255)
                    ccAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCa                                                                      >  1:56857/1‑93 (MQ=255)
                    acAGACACATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAACCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCa                                                                      <  1:138139/92‑1 (MQ=255)
                       gCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGca                                                                   >  1:744533/1‑93 (MQ=255)
                         cAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGcaca                                                                 >  2:4045314/1‑93 (MQ=255)
                         cAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGcaca                                                                 <  2:2611105/93‑1 (MQ=255)
                           aaTCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAat                                                               >  2:5513728/1‑93 (MQ=255)
                           aaTCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAat                                                               >  1:5209210/1‑93 (MQ=255)
                           aaTCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAat                                                               >  2:505136/1‑93 (MQ=255)
                           aaTCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAat                                                               >  2:1995968/1‑93 (MQ=255)
                             acAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAatat                                                             <  2:4928060/92‑1 (MQ=255)
                              cAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATg                                                            >  1:1657788/1‑93 (MQ=255)
                               aaGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGa                                                           >  1:62909/1‑93 (MQ=255)
                                 gTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAAt                                                         >  2:5957778/1‑93 (MQ=255)
                                 gTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAAt                                                         <  2:752402/93‑1 (MQ=255)
                                 gTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAAt                                                         <  1:554018/93‑1 (MQ=255)
                                   aaGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAACATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCg                                                       <  2:3430529/93‑1 (MQ=255)
                                    aGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGc                                                      <  1:6045384/93‑1 (MQ=255)
                                     gCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGcc                                                     >  2:4831328/1‑93 (MQ=255)
                                     gCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGcc                                                     <  2:2722508/93‑1 (MQ=255)
                                     gCCGCGCCGGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGcc                                                     <  1:4789480/93‑1 (MQ=255)
                                      ccGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCa                                                    <  2:146205/93‑1 (MQ=255)
                                       cgcgCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAg                                                   <  2:2564168/93‑1 (MQ=255)
                                       cgcgCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAg                                                   <  1:3698815/93‑1 (MQ=255)
                                        gcgcCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGt                                                  >  2:4649266/1‑93 (MQ=255)
                                         cgcCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTc                                                 >  1:6128761/1‑93 (MQ=255)
                                         cgcCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTc                                                 <  1:258031/93‑1 (MQ=255)
                                          gcCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTcc                                                >  2:4224681/1‑93 (MQ=255)
                                          gcCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTcc                                                <  2:1500749/93‑1 (MQ=255)
                                          gcCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTcc                                                <  1:1717470/93‑1 (MQ=255)
                                           ccTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCg                                               >  1:3016265/1‑93 (MQ=255)
                                           ccTGCCCACATCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCg                                               <  1:4543259/93‑1 (MQ=255)
                                            cTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCgg                                              <  2:5824035/93‑1 (MQ=255)
                                              gCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTc                                            >  2:5554375/1‑93 (MQ=255)
                                              gCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTc                                            <  1:1839906/93‑1 (MQ=255)
                                               cccACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCt                                           >  2:3906010/1‑93 (MQ=255)
                                                ccACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTg                                          >  1:5102442/1‑93 (MQ=255)
                                                ccACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTg                                          >  2:4162700/1‑93 (MQ=255)
                                                ccACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTg                                          >  2:5779254/1‑93 (MQ=255)
                                                ccACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTg                                          >  1:3001175/1‑93 (MQ=255)
                                                 cacttcaacacttcaaACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGa                                         <  2:44767/93‑1 (MQ=255)
                                                 cacttcaacacttcaaACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGa                                         <  1:263193/93‑1 (MQ=255)
                                                  acttcaacacttcaaACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGaa                                        >  2:1756140/1‑93 (MQ=255)
                                                  acttcaacacttcaaACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGaa                                        >  1:5478617/1‑93 (MQ=255)
                                                    ttcaacacttcaaACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGc                                      <  2:4965603/93‑1 (MQ=255)
                                                    ttcaacacttcaaACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGc                                      <  1:3187289/93‑1 (MQ=255)
                                                     tcaacacttcaaACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGcc                                     >  1:4125323/1‑93 (MQ=255)
                                                       aacacttcaaACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCa                                   >  2:2178057/1‑93 (MQ=255)
                                                          acttcaaACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCa                                >  2:1039101/1‑93 (MQ=255)
                                                           cttcaaACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCaa                               <  1:3832896/93‑1 (MQ=255)
                                                            ttcaaACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAAt                              <  1:1457053/93‑1 (MQ=255)
                                                             tcaaACGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATa                             <  2:6099150/93‑1 (MQ=255)
                                                               aaaCGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCCACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGa                           >  1:2697074/1‑93 (MQ=255)
                                                               aaaCGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGa                           <  1:4980078/93‑1 (MQ=255)
                                                                aaCGCAATCACTGGGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGAc                          <  1:1140089/93‑1 (MQ=255)
                                                                aaCGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGAc                          >  2:5136330/1‑93 (MQ=255)
                                                                  cGCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGc                        <  2:3377785/93‑1 (MQ=255)
                                                                   gCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGcc                       <  2:4412685/93‑1 (MQ=255)
                                                                   gCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGcc                       <  2:248600/93‑1 (MQ=255)
                                                                   gCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGcc                       >  2:1898973/1‑93 (MQ=255)
                                                                   gCAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGcc                       <  2:5268269/93‑1 (MQ=255)
                                                                    cAATCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGAAATAGACGCCa                      >  1:2122214/1‑93 (MQ=255)
                                                                     aaTCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCAc                     >  2:795386/1‑93 (MQ=255)
                                                                      aTCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCAcc                    <  1:108688/93‑1 (MQ=255)
                                                                      aTCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCAcc                    >  1:3570491/1‑93 (MQ=255)
                                                                       tCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCAccg                   >  2:2297447/1‑93 (MQ=255)
                                                                       tCACTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCAccg                   <  1:5339073/93‑1 (MQ=255)
                                                                          cTGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCAccgcgc                <  2:1254356/93‑1 (MQ=255)
                                                                           tGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCAccgcgcc               >  2:2073059/1‑93 (MQ=255)
                                                                           tGAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCAccgcgcc               >  1:409790/1‑93 (MQ=255)
                                                                            gAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCAccgcgccg              >  2:3180874/1‑93 (MQ=255)
                                                                            gAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCAccgcgccg              <  2:3996415/93‑1 (MQ=255)
                                                                            gAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCAccgcgccg              >  2:1881303/1‑93 (MQ=255)
                                                                            gAGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCAccgcgccg              >  2:4422080/1‑93 (MQ=255)
                                                                             aGCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCAccgcgccgc             >  2:639850/1‑93 (MQ=255)
                                                                              gCGGGAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCAccgcgccgcg            >  1:530254/1‑93 (MQ=255)
                                                                                gggAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGAt          <  1:3392494/93‑1 (MQ=255)
                                                                                gggAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGAt          >  2:930210/1‑93 (MQ=255)
                                                                                 ggAAAATAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATa         >  2:2902163/1‑93 (MQ=255)
                                                                                    aaaTAGCGTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAAcc      >  2:3322019/1‑93 (MQ=255)
                                                                                             |                                                                                       
GGCGCGCCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGCGT‑CCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGG  >  NC_000913/4006388‑4006567

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGCGCCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GT‑C‑C‑CAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAG  >  NC_000913/4006390‑4006576
                                                                                                 |                                                                                             
CGCGCCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GT‑C‑C‑C                                                                                               >  SRR4302534.3733876/1‑94 (MQ=60)
 GCGCCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GT‑C‑C‑CC                                                                                              >  SRR4302534.100593/1‑94 (MQ=60)
  TGCCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GT‑C‑C‑CCA                                                                                             >  SRR4302534.3589513/1‑94 (MQ=60)
  CGCCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GT‑C‑C‑CCA                                                                                             <  SRR4302534.5083296/94‑1 (MQ=60)
  CGCCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GT‑C‑C‑CCA                                                                                             >  SRR4302534.81136/1‑94 (MQ=60)
   GCCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GT‑C‑C‑Ccaa                                                                                            >  SRR4302534.2258971/1‑91 (MQ=60)
   GCCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GT‑C‑C‑Ccaa                                                                                            >  SRR4302534.2114149/1‑91 (MQ=60)
    CCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCC‑C‑CAAC                                                                                            <  SRR4302534.5657964/94‑1 (MQ=60)
    CCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCC‑C‑CAAC                                                                                            <  SRR4302534.6609083/94‑1 (MQ=60)
     CACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCC‑C‑CAACC                                                                                           <  SRR4302534.3083009/94‑1 (MQ=60)
       CTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCC‑C‑CAACCCC                                                                                         >  SRR4302534.6089196/1‑94 (MQ=60)
       CTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCC‑C‑CAACCCC                                                                                         >  SRR4302534.899184/1‑94 (MQ=60)
        TGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCC‑C‑CAACCCCA                                                                                        <  SRR4302534.5259302/94‑1 (MQ=60)
         GCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCC‑C‑CAACCCCAC                                                                                       <  SRR4302534.5243426/94‑1 (MQ=60)
          CTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCC‑C‑CAACCCCACG                                                                                      <  SRR4302534.1786054/94‑1 (MQ=60)
           TGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCC‑C‑CAACCCCACGC                                                                                     >  SRR4302534.838253/1‑94 (MQ=60)
              ATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCC‑C‑CAACCCCACGCCAA                                                                                  >  SRR4302534.228576/1‑94 (MQ=60)
              ATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTAAACACTTCAAACGCAATCACTGAGCGGGAAACTAGC‑GTCC‑C‑CAACCCCACGCCAA                                                                                  >  SRR4302534.2100452/1‑94 (MQ=60)
               ttcgcagatAAATCAAGTAAGCAGCGCATGCCCACTACACCACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GA‑C‑CACAGCCCCACGCCAAC                                                                                 <  SRR4302534.3788072/85‑1 (MQ=53)
               TTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCC‑C‑CAACCCCACGCCAAC                                                                                 >  SRR4302534.1854921/1‑94 (MQ=60)
                 CCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCC‑C‑CAACCCCACGCCAACCA                                                                               >  SRR4302534.58393/1‑94 (MQ=60)
                 CCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCC‑C‑CAACCCCACGCCAACCA                                                                               <  SRR4302534.6217403/94‑1 (MQ=60)
                  acagacacATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAACCACTGAGCGGGAAAATAGC‑GTCC‑C‑CAACCCCACGCCAACCAG                                                                              <  SRR4302534.141749/86‑1 (MQ=60)
                    AGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCC‑C‑CAACCCCACGCCAACCAGCA                                                                            <  SRR4302534.5108011/94‑1 (MQ=60)
                    AGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCC‑C‑CAACCCCACGCCAACCAGCA                                                                            >  SRR4302534.762983/1‑94 (MQ=60)
                    AGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCC‑C‑CAACCCCACGCCAACCAGCA                                                                            >  SRR4302534.5865648/1‑94 (MQ=60)
                        AAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCC‑C‑CAACCCCACGCCAACCAGCACAAT                                                                        >  SRR4302534.5349795/1‑94 (MQ=60)
                        AAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCC‑C‑CAACCCCACGCCAACCAGCACAAT                                                                        <  SRR4302534.2872276/94‑1 (MQ=60)
                         AATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCC‑C‑CAACCCCACGCCAACCAGCACAATA                                                                       <  SRR4302534.5502403/94‑1 (MQ=60)
                          ATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCC‑C‑CAACCCCACGCCAACCAGCACAATAT                                                                      <  SRR4302534.4332700/94‑1 (MQ=60)
                           TCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCC‑C‑CAACCCCACGCCAACCAGCACAATATG                                                                     >  SRR4302534.1700980/1‑94 (MQ=60)
                            CAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCC‑C‑CAACCCCACGCCAACCAGCACAATATGA                                                                    >  SRR4302534.64621/1‑94 (MQ=60)
                              AGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCC‑C‑CAACCCCACGCCAACCAGCACAATATGAAT                                                                  >  SRR4302534.872515/1‑94 (MQ=60)
                              AGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCC‑C‑CAACCCCACGCCAACCAGCACAATATGAAT                                                                  <  SRR4302534.1704678/94‑1 (MQ=60)
                               GTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCC‑C‑CAACCCCACGCCAACCAGCACAATATGAATC                                                                 <  SRR4302534.567680/94‑1 (MQ=60)
                               GTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCC‑C‑CAACCCCACGCCAACCAGCACAATATGAATC                                                                 <  SRR4302534.1732676/94‑1 (MQ=60)
                               GTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCC‑C‑CAACCCCACGCCAACCAGCACAATATGAATC                                                                 >  SRR4302534.3666501/1‑94 (MQ=60)
                                  AGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCC‑C‑CAACCCCACGCCAACCAGCACAATATGAATCGCC                                                              <  SRR4302534.6209716/94‑1 (MQ=60)
                                   GCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCC‑C‑CAACCCCACGCCAACCAGCACAATATGAATCGCCA                                                             <  SRR4302534.5065967/94‑1 (MQ=60)
                                   GCCGCGCCGGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCC‑C‑CAACCCCACGCCAACCAGCACAATATGAATCGCCA                                                             <  SRR4302534.4919527/94‑1 (MQ=60)
                                     CGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCC‑C‑CAACCCCACGCCAACCAGCACAATATGAATCGCCAGT                                                           <  SRR4302534.3798257/94‑1 (MQ=60)
                                     CGAGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GT‑CAC‑CAACCGCACGCCAACCAGCACAATATGAATtggcctc                                                           >  SRR4302534.4113343/1‑87 (MQ=60)
                                      GCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCC‑C‑CAACCCCACGCCAACCAGCACAATATGAATCGCCAGTC                                                          >  SRR4302534.6295468/1‑94 (MQ=60)
                                       CGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCC‑C‑CAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCC                                                         >  SRR4302534.3235040/1‑94 (MQ=60)
                                       CGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCC‑C‑CAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCC                                                         >  SRR4302534.1871005/1‑94 (MQ=60)
                                       CGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCC‑C‑CAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCC                                                         <  SRR4302534.1451333/94‑1 (MQ=60)
                                       CGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCC‑C‑CAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCC                                                         <  SRR4302534.264752/94‑1 (MQ=60)
                                       CGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCC‑C‑CAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCC                                                         >  SRR4302534.2663852/1‑94 (MQ=60)
                                        GCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCC‑C‑CAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCG                                                        <  SRR4302534.1762086/94‑1 (MQ=60)
                                        GCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCC‑C‑CAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCG                                                        >  SRR4302534.3095228/1‑94 (MQ=60)
                                         CCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCC‑C‑CAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGG                                                       <  SRR4302534.3828318/94‑1 (MQ=60)
                                         CCTGCCCACATCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCC‑C‑CAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGG                                                       <  SRR4302534.4666415/94‑1 (MQ=60)
                                           TGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCC‑C‑CAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTC                                                     >  SRR4302534.759513/1‑94 (MQ=60)
                                            GCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCC‑C‑CAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCT                                                    <  SRR4302534.1887734/94‑1 (MQ=60)
                                             CCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCC‑C‑CAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTG                                                   >  SRR4302534.3079676/1‑94 (MQ=60)
                                             CCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCC‑C‑CAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTG                                                   >  SRR4302534.5240165/1‑94 (MQ=60)
                                               CACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCC‑C‑CAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAA                                                 <  SRR4302534.270017/94‑1 (MQ=60)
                                               CACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCC‑C‑CAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAA                                                 >  SRR4302534.5626351/1‑94 (MQ=60)
                                               CACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCC‑C‑CAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAA                                                 >  SRR4302534.955870/1‑94 (MQ=60)
                                                ACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCC‑C‑CAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAG                                                >  SRR4302534.5465269/1‑94 (MQ=60)
                                                 CTTCAACACTTCACACGCAATCACTGAGCGGGAAAATAGCGGC‑C‑C‑CAAACCCACCACAACCAGCACAATATGAATCGCCAGTCCGATCAGAAGC                                               >  SRR4302534.6520031/1‑94 (MQ=60)
                                                  TTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCC‑C‑CAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCC                                              >  SRR4302534.4236375/1‑94 (MQ=60)
                                                  TTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCC‑C‑CAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCC                                              <  SRR4302534.3271630/94‑1 (MQ=60)
                                                   TCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCC‑C‑CAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCC                                             <  SRR4302534.1018215/94‑1 (MQ=60)
                                                   TCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCC‑C‑CAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCC                                             <  SRR4302534.1963675/94‑1 (MQ=60)
                                                    CAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCC‑C‑CAACCCCACGCC‑ACCAGCACCAAAATAATCGCCAAGCCCGTCTGAAGCCCAG                                           >  SRR4302534.2190754/1‑94 (MQ=48)
                                                    CAACACTTCAAACGCAATCACTGAGCGGGAAAATAGA‑GTCC‑C‑CAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCA                                            >  SRR4302534.6316024/1‑94 (MQ=60)
                                                      ACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCC‑C‑CAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGC                                          <  SRR4302534.2744072/94‑1 (MQ=60)
                                                      ACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCC‑C‑CAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGC                                          <  SRR4302534.5835829/94‑1 (MQ=60)
                                                         CTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCC‑C‑CAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAAT                                       <  SRR4302534.5232591/94‑1 (MQ=60)
                                                         CTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCC‑C‑CAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAAT                                       <  SRR4302534.3935829/94‑1 (MQ=60)
                                                          TTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCC‑C‑CAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATA                                      <  SRR4302534.1495379/94‑1 (MQ=60)
                                                            CAAACGCAATCACTGAGCGGGAAAATAGC‑GTCC‑C‑CAACCCCACGCCCACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGA                                    >  SRR4302534.2768328/1‑94 (MQ=60)
                                                            CAAACGCAATCACTGAGCGGGAAAATAGC‑GTCC‑C‑CAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGA                                    <  SRR4302534.4725804/94‑1 (MQ=60)
                                                            CAAACGCAATCACTGAGCGGGAAAATAGC‑GTCC‑C‑CAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGA                                    >  SRR4302534.4182391/1‑94 (MQ=60)
                                                             AAACGCAATCACTGAGCGGGAAAATAGC‑GTCC‑C‑CAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGAC                                   <  SRR4302534.5114869/94‑1 (MQ=60)
                                                             AAACGCAATCACTGAGCGGGAAAATAGC‑GTCC‑C‑CAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGAC                                   >  SRR4302534.5635410/1‑94 (MQ=60)
                                                              AACGCAATCACTGGGCGGGAAAATAGC‑GTCC‑C‑CAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACG                                  <  SRR4302534.1169921/94‑1 (MQ=60)
                                                                 GCAATCACTGAGCGGGAAAATAGC‑GTCC‑C‑CAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCA                               >  SRR4302534.3561226/1‑94 (MQ=60)
                                                                 GCAATCACTGAGCGGGAAAATAGC‑GTCC‑C‑CAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGAAATAGACGCCA                               >  SRR4302534.2178095/1‑94 (MQ=60)
                                                                   AATCACTGAGCGGGAAAATAGC‑GTCC‑C‑CAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGCCCCCACC                             >  SRR4302534.4103723/1‑94 (MQ=60)
                                                                   AATCACTGAGCGGGAAAATAGC‑GTCC‑C‑CAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACC                             >  SRR4302534.3666525/1‑94 (MQ=60)
                                                                    ATCACTGAGCGGGAAAATAGC‑GTCC‑C‑CAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCG                            <  SRR4302534.5671136/94‑1 (MQ=60)
                                                                    ATCACTGAGCGGGAAAATAGC‑GTCC‑C‑CAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCG                            <  SRR4302534.111591/94‑1 (MQ=60)
                                                                     TCACTGAGCGGGAAAATAGC‑GTCC‑C‑CAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGC                           <  SRR4302534.5483023/94‑1 (MQ=60)
                                                                     TCACTGAGCGGGAAAATAGC‑GTCC‑C‑CAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGC                           <  SRR4302534.6141268/94‑1 (MQ=60)
                                                                      CACTGAGCGGGAAAATAGC‑GTCC‑C‑CAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCG                          <  SRR4302534.15603/94‑1 (MQ=60)
                                                                        CTGAGCGGGAAAATAGC‑GTCC‑C‑CAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCC                        >  SRR4302534.3024810/1‑94 (MQ=60)
                                                                        CTGAGCGGGAAAATAGC‑GTCC‑C‑CAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCC                        >  SRR4302534.420269/1‑94 (MQ=60)
                                                                          GAGCGGGAAAATAGC‑GTCC‑C‑CAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGC                      <  SRR4302534.5336059/94‑1 (MQ=60)
                                                                           GGCGGGAAAATAGC‑GTCC‑C‑CAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCG                     >  SRR4302534.3409607/1‑94 (MQ=60)
                                                                           AGCGGGAAAATAGC‑GTCC‑C‑CAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCG                     >  SRR4302534.543426/1‑94 (MQ=60)
                                                                             CGGGAAAATAGC‑GTCC‑C‑CAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGAT                   >  SRR4302534.1388920/1‑94 (MQ=60)
                                                                             CGGGAAAATAGC‑GTCC‑C‑CAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGAT                   >  SRR4302534.6518727/1‑94 (MQ=60)
                                                                              GGGAAAATAGC‑GTCC‑C‑CAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATA                  <  SRR4302534.3483499/94‑1 (MQ=60)
                                                                               GGAAAATAGC‑GTCC‑C‑CAACCCCACGCCAACCAGCACAATATGAATTGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAA                 >  SRR4302534.810543/1‑94 (MQ=60)
                                                                                  AAATAGC‑GTCC‑C‑CAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCG              <  SRR4302534.5360193/94‑1 (MQ=60)
                                                                                   AATAGC‑GTCC‑C‑CAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGT             >  SRR4302534.237856/1‑94 (MQ=60)
                                                                                      AGC‑GTCC‑C‑CAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGT          >  SRR4302534.3381642/1‑94 (MQ=60)
                                                                                      AGC‑GTCC‑C‑CAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGT          >  SRR4302534.3713337/1‑94 (MQ=60)
                                                                                        C‑GTCC‑C‑CAATCCCACGGCAACCAGCACAATATGAATCGCCAGTCCGGTCTGACGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGAGGTTG        >  SRR4302534.6434401/1‑94 (MQ=60)
                                                                                         cggcC‑C‑CAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTG        <  SRR4302534.1466413/90‑1 (MQ=60)
                                                                                          gtcC‑C‑CAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGA       <  SRR4302534.4712587/91‑1 (MQ=60)
                                                                                          gtcC‑C‑CAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGA       >  SRR4302534.6590106/4‑94 (MQ=60)
                                                                                           C‑C‑C‑CAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAAACGTGGTTGAGC     >  SRR4302534.3311592/1‑94 (MQ=60)
                                                                                             C‑C‑CAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCG    >  SRR4302534.2411035/1‑94 (MQ=60)
                                                                                               C‑CAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGA   >  SRR4302534.1378432/1‑94 (MQ=60)
                                                                                                 CAACCCCACGCCAACCAGAACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACTCTACCGCGCCGCGATAACCGTGGTTGAGCGAG  <  SRR4302534.6281336/94‑1 (MQ=60)
                                                                                                 |                                                                                             
CGCGCCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GT‑C‑C‑CAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGTTGAGCGAG  >  NC_000913/4006390‑4006576

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑