Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	884	257.2	12958910	96.9%	12557183	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,016,743	C→T	P101L (CCC→CTC)	ubiE →	UbiE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,016,743	0	C	T	100.0%	906.2 / NA	232	P101L (CCC→CTC)	ubiE	UbiE
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (120/112); total (120/112)

Only 100 of 235 total aligned reads displayed.

CTGGTGGCACCGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCCCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGG  >  NC_000913/4016653‑4016832

       cACCGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATggg                                                                                  <  1:3221755/93‑1 (MQ=255)

        cccGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGc                                                                                 <  1:4461605/92‑1 (MQ=255)

         ccGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGcc                                                                                >  1:2155780/1‑93 (MQ=255)

         ccGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGcc                                                                                <  2:3599617/93‑1 (MQ=255)

          cGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCcg                                                                               <  1:2016284/93‑1 (MQ=255)

           ggCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCcgc                                                                              <  2:435855/93‑1 (MQ=255)

           ggCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCcgc                                                                              <  2:3806578/93‑1 (MQ=255)

            gCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCcgcg                                                                             >  2:4669474/1‑93 (MQ=255)

             cGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCACAATGGGCCGCga                                                                            <  1:5152784/93‑1 (MQ=255)

              gACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCgag                                                                           <  1:3936793/93‑1 (MQ=255)

                ccTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGaa                                                                         >  1:4616771/1‑93 (MQ=255)

                  tGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGc                                                                       >  2:1039472/1‑93 (MQ=255)

                   gACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCt                                                                      <  1:5708856/93‑1 (MQ=255)

                   gACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCt                                                                      >  1:4695064/1‑93 (MQ=255)

                    aCAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTg                                                                     <  1:3052401/93‑1 (MQ=255)

                      aGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCg                                                                   >  1:5251837/1‑93 (MQ=255)

                       gCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCGATGATAAAAATGGGCCGAGAGAAGCTGCGt                                                                  >  2:3906468/1‑93 (MQ=255)

                       gCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGt                                                                  <  1:2950778/93‑1 (MQ=255)

                       gCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGt                                                                  <  2:5960400/93‑1 (MQ=255)

                        cGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTa                                                                 <  2:207414/93‑1 (MQ=255)

                        cGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTa                                                                 >  1:1886012/1‑93 (MQ=255)

                         gAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTaa                                                                >  1:6302311/1‑93 (MQ=255)

                          aaaTTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAat                                                               <  2:4457518/93‑1 (MQ=255)

                            aTTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAatat                                                             >  2:533144/1‑93 (MQ=255)

                            aTTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAatat                                                             >  1:4551361/1‑93 (MQ=255)

                            aTTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAatat                                                             <  1:10957/93‑1 (MQ=255)

                             ttCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATc                                                            <  1:5744675/93‑1 (MQ=255)

                               ctcCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCgg                                                          >  2:6122270/1‑93 (MQ=255)

                                tcCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGgt                                                         >  2:4738714/1‑93 (MQ=255)

                                tcCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGgt                                                         <  2:5135859/93‑1 (MQ=255)

                                tcCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGgt                                                         <  1:319145/93‑1 (MQ=255)

                                 cccGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGgtg                                                        >  1:6041908/1‑93 (MQ=255)

                                 cccGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGgtg                                                        <  2:5091582/93‑1 (MQ=255)

                                 cccGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGgtg                                                        >  1:228018/1‑93 (MQ=255)

                                    gCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGa                                                     >  2:5951868/1‑93 (MQ=255)

                                     ccTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGAt                                                    >  2:2788552/1‑93 (MQ=255)

                                      cTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGAtt                                                   <  1:3856689/93‑1 (MQ=255)

                                       tGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTg                                                  <  2:210971/93‑1 (MQ=255)

                                         gTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGc                                                <  2:550790/93‑1 (MQ=255)

                                          tCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCa                                               >  1:1510396/1‑93 (MQ=255)

                                           cGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCaa                                              >  1:5095193/1‑93 (MQ=255)

                                            ggAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAAc                                             >  2:3911071/1‑93 (MQ=255)

                                              agaAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGt                                           >  2:912305/1‑93 (MQ=255)

                                              agaAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGt                                           >  1:1398306/1‑93 (MQ=255)

                                               gaAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGtt                                          >  2:6231044/1‑93 (MQ=255)

                                               gaAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGtt                                          >  2:677538/1‑93 (MQ=255)

                                                 aaCTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGa                                        >  2:2900620/1‑93 (MQ=255)

                                                 aaCTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGa                                        <  2:5012675/93‑1 (MQ=255)

                                                 aaCTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGa                                        >  1:1037957/1‑93 (MQ=255)

                                                 aaCTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGa                                        <  1:857200/93‑1 (MQ=255)

                                                    tGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAgca                                     <  2:565699/93‑3 (MQ=255)

                                                     ggCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCAt                                    <  2:5542532/93‑1 (MQ=255)

                                                     ggCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCAt                                    >  2:2513213/1‑93 (MQ=255)

                                                      gCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATg                                   <  2:4164691/93‑1 (MQ=255)

                                                      gCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATg                                   >  1:4799423/1‑93 (MQ=255)

                                                       cAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGt                                  <  1:3781377/93‑1 (MQ=255)

                                                       cAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGt                                  <  1:3840154/93‑1 (MQ=255)

                                                       cAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGt                                  >  2:4539247/1‑93 (MQ=255)

                                                         aaGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTc                                <  2:4700706/93‑1 (MQ=255)

                                                             ggTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGc                            <  1:4968826/93‑1 (MQ=255)

                                                             ggTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGc                            <  2:1358689/93‑1 (MQ=255)

                                                             ggTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGc                            <  2:397749/93‑1 (MQ=255)

                                                              gTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCg                           <  1:5464069/93‑1 (MQ=255)

                                                               tCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGa                          >  1:4119946/1‑93 (MQ=255)

                                                               tCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGa                          >  1:3343367/1‑93 (MQ=255)

                                                                ccTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGaa                         >  1:3706357/1‑93 (MQ=255)

                                                                 cTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCCGGCGAAc                        >  1:576316/1‑93 (MQ=255)

                                                                   tGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGc                      <  1:661190/93‑1 (MQ=255)

                                                                   tGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGc                      <  1:5438615/93‑1 (MQ=255)

                                                                    gCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGGGGACGCt                     >  2:1527113/1‑93 (MQ=255)

                                                                      tGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGa                   >  2:6428651/1‑93 (MQ=255)

                                                                      tGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGa                   >  1:6158116/1‑93 (MQ=255)

                                                                        atatCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAgg                 <  2:3247956/93‑1 (MQ=255)

                                                                        atatCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAgg                 >  2:5730617/1‑93 (MQ=255)

                                                                         tatCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGgc                <  1:2887846/93‑1 (MQ=255)

                                                                         tatCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGgc                <  2:1146915/93‑1 (MQ=255)

                                                                         tatCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGgc                >  2:5894621/1‑93 (MQ=255)

                                                                           tCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGgcgc              >  1:5082923/1‑93 (MQ=255)

                                                                           tCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGgcgc              <  1:2038499/93‑1 (MQ=255)

                                                                           tCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGgcgc              >  1:1000375/1‑93 (MQ=255)

                                                                           tCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGgcgc              >  1:80698/1‑93 (MQ=255)

                                                                            cAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCt             <  2:3148090/93‑1 (MQ=255)

                                                                             aaTGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTg            >  1:4206392/1‑93 (MQ=255)

                                                                             aaTGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTg            >  2:3136805/1‑93 (MQ=255)

                                                                              aTGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGc           <  2:4123721/93‑1 (MQ=255)

                                                                                gAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCg         >  2:2627318/1‑93 (MQ=255)

                                                                                 aaTCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGt        <  1:1494654/93‑1 (MQ=255)

                                                                                   tCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTc      >  1:3141720/1‑93 (MQ=255)

                                                                                   tCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTc      <  2:1731585/93‑1 (MQ=255)

                                                                                   tCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTc      >  2:481936/1‑93 (MQ=255)

                                                                                     cATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCCACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTccc    >  1:6374773/1‑93 (MQ=255)

                                                                                     cATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTccc    <  2:4428558/93‑1 (MQ=255)

                                                                                     cATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTccc    >  1:2510468/1‑93 (MQ=255)

                                                                                     cATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTccc    <  1:2735496/93‑1 (MQ=255)

                                                                                     cATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTccc    <  2:1938596/93‑1 (MQ=255)

                                                                                      aTGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCg   >  2:5218822/1‑93 (MQ=255)

                                                                                      aTGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCg   >  2:1278601/1‑93 (MQ=255)

                                                                                      aTGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCg   <  2:942294/93‑1 (MQ=255)

                                                                                       tGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCgg  >  1:1694340/1‑93 (MQ=255)

                                                                                       tGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCgg  >  1:5680027/1‑93 (MQ=255)

CTGGTGGCACCGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCCCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGG  >  NC_000913/4016653‑4016832

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TGGCTGGTGGCACCGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCCCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAA  >  NC_000913/4016650‑4016836

TGGCTGGTGGCACCGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCT                                                                                               <  SRR4302534.5313618/94‑1 (MQ=60)

 GGCTGGTGGCACCGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTC                                                                                              >  SRR4302534.1200717/1‑94 (MQ=60)

  GCTGGTGGCACCGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCA                                                                                             <  SRR4302534.5431427/94‑1 (MQ=60)

   CTGGTGACACAGCCGACCTGACAGCGAAATTCTGCAGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAA                                                                                            <  SRR4302534.3273861/94‑1 (MQ=60)

    TGGTGGCACCGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAA                                                                                           >  SRR4302534.5380220/1‑94 (MQ=60)

     GGTGGCACCGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAA                                                                                          >  SRR4302534.5917400/1‑94 (MQ=60)

          caggGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGC                                                                                     <  SRR4302534.3454984/90‑1 (MQ=60)

          CACCGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGC                                                                                     <  SRR4302534.3307304/94‑1 (MQ=60)

           CCCGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCC                                                                                    <  SRR4302534.4582236/94‑1 (MQ=60)

           ACCGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCC                                                                                    >  SRR4302534.2212689/1‑94 (MQ=60)

           ACCGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCC                                                                                    >  SRR4302534.2563069/1‑94 (MQ=60)

           ACCGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCC                                                                                    <  SRR4302534.633603/94‑1 (MQ=60)

            CCGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTTGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCG                                                                                   <  SRR4302534.5187186/94‑1 (MQ=60)

             CGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGC                                                                                  <  SRR4302534.2069058/94‑1 (MQ=60)

              GGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCG                                                                                 <  SRR4302534.4882769/94‑1 (MQ=60)

                CGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCACAATGGGCCGCGAG                                                                               <  SRR4302534.5291774/94‑1 (MQ=60)

                CGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAG                                                                               >  SRR4302534.3901988/1‑94 (MQ=60)

                CGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAG                                                                               <  SRR4302534.2084351/94‑1 (MQ=60)

                 GACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGA                                                                              <  SRR4302534.4042242/94‑1 (MQ=60)

                 GACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGA                                                                              >  SRR4302534.2560075/1‑94 (MQ=60)

                  ACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAA                                                                             >  SRR4302534.4742050/1‑94 (MQ=60)

                  ACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAA                                                                             <  SRR4302534.5633823/94‑1 (MQ=60)

                   CCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAG                                                                            <  SRR4302534.4764876/94‑1 (MQ=60)

                     TGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCT                                                                          >  SRR4302534.4822499/1‑94 (MQ=60)

                     TGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCT                                                                          <  SRR4302534.4098461/94‑1 (MQ=60)

                      GACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTG                                                                         >  SRR4302534.4361767/1‑94 (MQ=60)

                      GACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTG                                                                         <  SRR4302534.3181818/94‑1 (MQ=60)

                      GACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTG                                                                         <  SRR4302534.4886025/94‑1 (MQ=60)

                      GACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTG                                                                         <  SRR4302534.5863521/94‑1 (MQ=60)

                       ACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGC                                                                        >  SRR4302534.1518797/1‑94 (MQ=60)

                       ACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGC                                                                        <  SRR4302534.3132484/94‑1 (MQ=60)

                       ACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGC                                                                        >  SRR4302534.3010196/1‑94 (MQ=60)

                        CAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCG                                                                       >  SRR4302534.5393747/1‑94 (MQ=60)

                          GCGAACTTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTA                                                                     <  SRR4302534.742584/94‑1 (MQ=60)

                          GCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTA                                                                     >  SRR4302534.5607549/1‑94 (MQ=60)

                          GCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTA                                                                     <  SRR4302534.5918109/94‑1 (MQ=60)

                          GCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTA                                                                     <  SRR4302534.3028162/94‑1 (MQ=60)

                          GCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTA                                                                     >  SRR4302534.1935179/1‑94 (MQ=60)

                          GCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTA                                                                     <  SRR4302534.2179418/94‑1 (MQ=60)

                           CGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAA                                                                    >  SRR4302534.6472744/1‑94 (MQ=60)

                            GAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAAA                                                                   <  SRR4302534.6509528/94‑1 (MQ=60)

                              AATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATAT                                                                 >  SRR4302534.4674730/1‑94 (MQ=60)

                               ATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATC                                                                <  SRR4302534.11232/94‑1 (MQ=60)

                                TTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCG                                                               >  SRR4302534.3907834/1‑94 (MQ=60)

                                TTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCG                                                               <  SRR4302534.5900413/94‑1 (MQ=60)

                                   TCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTG                                                            >  SRR4302534.6206139/1‑94 (MQ=60)

                                   TCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTG                                                            <  SRR4302534.327372/94‑1 (MQ=60)

                                   TCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTG                                                            >  SRR4302534.233980/1‑94 (MQ=60)

                                    CCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGT                                                           >  SRR4302534.3290831/1‑94 (MQ=60)

                                      CGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGA                                                         >  SRR4302534.5874984/1‑94 (MQ=60)

                                      CGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGA                                                         >  SRR4302534.1408902/1‑94 (MQ=60)

                                        CCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATT                                                       >  SRR4302534.4737001/1‑94 (MQ=60)

                                        CCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATT                                                       >  SRR4302534.1031887/1‑94 (MQ=60)

                                        CCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATT                                                       <  SRR4302534.5113459/94‑1 (MQ=60)

                                         CTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTG                                                      >  SRR4302534.3008794/1‑94 (MQ=60)

                                         CTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTG                                                      <  SRR4302534.3960230/94‑1 (MQ=60)

                                           GGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGC                                                    <  SRR4302534.3833633/94‑1 (MQ=60)

                                           GGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGC                                                    <  SRR4302534.5225129/94‑1 (MQ=60)

                                            GTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCA                                                   >  SRR4302534.1550108/1‑94 (MQ=60)

                                             TCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAA                                                  >  SRR4302534.5232722/1‑94 (MQ=60)

                                              CGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAAC                                                 >  SRR4302534.1099962/1‑94 (MQ=60)

                                              CGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAAC                                                 >  SRR4302534.4304901/1‑94 (MQ=60)

                                                GAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGT                                               >  SRR4302534.1435096/1‑94 (MQ=60)

                                                   AAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGG                                            >  SRR4302534.4670488/1‑94 (MQ=60)

                                                   AAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGA                                            >  SRR4302534.1064742/1‑94 (MQ=60)

                                                    AACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAG                                           <  SRR4302534.878831/94‑1 (MQ=60)

                                                     ACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGC                                          >  SRR4302534.2068372/1‑94 (MQ=60)

                                                        GGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATG                                       >  SRR4302534.4929747/1‑94 (MQ=60)

                                                          CAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTT                                     <  SRR4302534.3956379/94‑1 (MQ=60)

                                                          CAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTT                                     <  SRR4302534.3943304/94‑1 (MQ=60)

                                                          CAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTT                                     <  SRR4302534.3882902/94‑1 (MQ=60)

                                                          CAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTT                                     >  SRR4302534.3340199/1‑94 (MQ=60)

                                                               TGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGC                                >  SRR4302534.654507/1‑94 (MQ=60)

                                                               TGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGC                                >  SRR4302534.3343840/1‑94 (MQ=60)

                                                                GGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCG                               <  SRR4302534.3256341/94‑1 (MQ=60)

                                                                GGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCG                               <  SRR4302534.5103360/94‑1 (MQ=60)

                                                                GGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCG                               <  SRR4302534.4674187/94‑1 (MQ=60)

                                                                 GTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGA                              >  SRR4302534.4230811/1‑94 (MQ=60)

                                                                 GTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGA                              >  SRR4302534.3432852/1‑94 (MQ=60)

                                                                 GTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGA                              >  SRR4302534.5092455/1‑94 (MQ=60)

                                                                 GTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGA                              >  SRR4302534.1547939/1‑94 (MQ=60)

                                                                 GTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGA                              >  SRR4302534.223602/1‑94 (MQ=60)

                                                                 GTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGA                              <  SRR4302534.5611347/94‑1 (MQ=60)

                                                                  TCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAA                             >  SRR4302534.3805997/1‑94 (MQ=60)

                                                                   CCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCCGGCGAAC                            >  SRR4302534.590461/1‑94 (MQ=60)

                                                                    CTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACG                           >  SRR4302534.743249/1‑94 (MQ=60)

                                                                    CTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACG                           <  SRR4302534.5927588/94‑1 (MQ=60)

                                                                    CTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACG                           <  SRR4302534.2866631/94‑1 (MQ=60)

                                                                      TGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCT                         <  SRR4302534.5585083/94‑1 (MQ=60)

                                                                      TGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCT                         <  SRR4302534.677266/94‑1 (MQ=60)

                                                                       GCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTG                        <  SRR4302534.5821142/94‑1 (MQ=60)

                                                                        CTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGA                       <  SRR4302534.1564545/94‑1 (MQ=60)

                                                                        CTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGA                       >  SRR4302534.6325615/1‑94 (MQ=60)

                                                                         TGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAG                      <  SRR4302534.4045694/94‑1 (MQ=60)

                                                                         TGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCCTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAG                      >  SRR4302534.546279/1‑94 (MQ=60)

                                                                          GATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGG                     >  SRR4302534.1294116/1‑94 (MQ=60)

                                                                          GATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGGTCAGGCGAACGCTGAGG                     >  SRR4302534.1884153/1‑94 (MQ=60)

                                                                           ATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGC                    <  SRR4302534.527515/94‑1 (MQ=60)

                                                                            TATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCG                   <  SRR4302534.2963620/94‑1 (MQ=60)

                                                                            GATCAATGAATCCATGCCGAATATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTAAGGCG                   <  SRR4302534.5079004/94‑1 (MQ=60)

                                                                             ATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGC                  >  SRR4302534.1026014/1‑94 (MQ=60)

                                                                             ATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGC                  >  SRR4302534.82871/1‑94 (MQ=60)

                                                                             ATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGC                  >  SRR4302534.5220113/1‑94 (MQ=60)

                                                                              TCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCT                 <  SRR4302534.2091801/94‑1 (MQ=60)

                                                                               CAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTG                >  SRR4302534.4319929/1‑94 (MQ=60)

                                                                               CAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTG                >  SRR4302534.877443/1‑94 (MQ=60)

                                                                                 ATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCATGCGAACGCTGAGGCGCTGCC              <  SRR4302534.6408353/94‑1 (MQ=60)

                                                                                  TGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCG             <  SRR4302534.3052292/94‑1 (MQ=60)

                                                                                   GAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGT            >  SRR4302534.5847779/1‑94 (MQ=60)

                                                                                   GAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGT            >  SRR4302534.2070536/1‑94 (MQ=60)

                                                                                    AATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAAGGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTT           <  SRR4302534.6318301/94‑1 (MQ=60)

                                                                                    AATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTT           <  SRR4302534.5428883/94‑1 (MQ=60)

                                                                                    AATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTT           <  SRR4302534.1533972/94‑1 (MQ=60)

                                                                                     ATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTC          <  SRR4302534.5225854/94‑1 (MQ=60)

                                                                                     ATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTC          >  SRR4302534.3224351/1‑94 (MQ=60)

                                                                                       CCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCCACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCC        >  SRR4302534.6546924/1‑94 (MQ=60)

                                                                                       CCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCC        >  SRR4302534.1721371/1‑94 (MQ=60)

                                                                                       CCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCC        <  SRR4302534.3987145/94‑1 (MQ=60)

                                                                                       CCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCC        >  SRR4302534.2577120/1‑94 (MQ=60)

                                                                                        CATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCG       <  SRR4302534.2807604/94‑1 (MQ=60)

                                                                                         ATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGG      >  SRR4302534.5833823/1‑94 (MQ=60)

                                                                                         ATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGG      >  SRR4302534.2799697/1‑94 (MQ=60)

                                                                                         ATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGG      >  SRR4302534.1738384/1‑94 (MQ=60)

                                                                                           GCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGAT    <  SRR4302534.84151/94‑1 (MQ=60)

                                                                                           GCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGAT    <  SRR4302534.1295507/94‑1 (MQ=60)

                                                                                           GCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGAT    <  SRR4302534.4866523/94‑1 (MQ=60)

                                                                                             TCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGTTGAGGCGCTGCCGTTCCCGGATAA  <  SRR4302534.5321881/94‑1 (MQ=60)

                                                                                             TCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAA  <  SRR4302534.808092/94‑1 (MQ=60)

                                                                                             TCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAA  <  SRR4302534.2417689/94‑1 (MQ=60)

TGGCTGGTGGCACCGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCCCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAA  >  NC_000913/4016650‑4016836

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 7 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`