Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	884	257.2	12958910	96.9%	12557183	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,021,684	Δ1 bp	coding (542/609 nt)\|coding (1/243 nt)	b4556 → tatD →	hypothetical protein Mg‑dependent DNase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,021,683	0	C	.	99.6%	1034.2 / ‑3.9	221	coding (541/609 nt)	b4556	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (0/0); major base . (98/122); minor base G (0/1); total (98/123)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 240 total aligned reads displayed.

TTCTTCATTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAACCGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTCCCTC  >  NC_000913/4021591‑4021771

ttcttcATTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAAc                                                                                          <  1:5968786/93‑1 (MQ=255)

ttcttcATTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAAc                                                                                          >  1:179875/1‑93 (MQ=255)

 tcttcATTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAAcg                                                                                         <  1:1884618/93‑2 (MQ=255)

 tcttcATTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAAcg                                                                                         >  2:483314/1‑92 (MQ=255)

  cttcATTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAAcga                                                                                        <  1:802566/93‑3 (MQ=255)

   ttcATTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAAcgac                                                                                       >  1:5992265/1‑90 (MQ=255)

    tcATTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAAcgacg                                                                                      >  1:3439456/1‑89 (MQ=255)

    tcATTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAAcgacg                                                                                      <  2:5724903/93‑5 (MQ=255)

    tcATTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAAcgacg                                                                                      >  1:337499/1‑89 (MQ=255)

        tGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGa                                                                                 <  2:1658591/93‑1 (MQ=255)

         gCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGAc                                                                                >  2:6112542/1‑93 (MQ=255)

         gCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGAc                                                                                <  1:3794235/93‑1 (MQ=255)

          cTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACt                                                                               >  2:2062345/1‑93 (MQ=255)

           tttACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTg                                                                              <  1:4932014/93‑1 (MQ=255)

           tttACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTg                                                                              <  2:4161869/93‑1 (MQ=255)

            ttACCGGCACACGCGAAGATATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTgg                                                                             <  1:1132846/93‑1 (MQ=255)

            ttACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTgg                                                                             <  1:5105918/93‑1 (MQ=255)

             tACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGa                                                                            <  1:5721740/93‑1 (MQ=255)

              aCCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAg                                                                           >  1:5536817/1‑93 (MQ=255)

                cGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCt                                                                         <  2:5168980/93‑1 (MQ=255)

                  gCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGc                                                                       <  1:691480/93‑1 (MQ=255)

                  gCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGc                                                                       <  2:3920443/93‑1 (MQ=255)

                    acacGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCgg                                                                     >  2:5019231/1‑93 (MQ=255)

                    acacGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCgg                                                                     <  1:5394980/93‑1 (MQ=255)

                     cacGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCggg                                                                    >  1:4201346/1‑93 (MQ=255)

                      acGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGa                                                                   >  1:1537466/1‑93 (MQ=255)

                       cgcgAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGaa                                                                  <  2:3819054/93‑1 (MQ=255)

                       cgcgAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGaa                                                                  <  2:1365611/93‑1 (MQ=255)

                        gcgAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGaaa                                                                 <  1:4502831/93‑2 (MQ=255)

                        gcgAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAAc                                                                 <  2:3280184/93‑1 (MQ=255)

                         cgAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACt                                                                >  1:3268277/1‑93 (MQ=255)

                         cgAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACt                                                                <  1:667863/93‑1 (MQ=255)

                          gAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACtt                                                               <  1:2287495/93‑1 (MQ=255)

                          gAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACtt                                                               <  1:3219060/93‑1 (MQ=255)

                           aaGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACttt                                                              <  2:602687/93‑1 (MQ=255)

                           aaGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACttt                                                              >  1:780699/1‑93 (MQ=255)

                           aaGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACttt                                                              >  2:1142676/1‑93 (MQ=255)

                            agagATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACtttt                                                             >  2:781812/1‑93 (MQ=255)

                             gagaTGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTg                                                            >  1:6254185/1‑93 (MQ=255)

                                 tGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGt                                                        <  2:5090849/93‑1 (MQ=255)

                                 tGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGt                                                        <  1:6063091/93‑1 (MQ=255)

                                 tGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGt                                                        <  1:1710004/93‑1 (MQ=255)

                                  gCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGtt                                                       <  2:5207975/93‑1 (MQ=255)

                                  gCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGtt                                                       >  2:6402185/1‑93 (MQ=255)

                                      gcgtgcgtGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGAtt                                                   <  2:5865185/93‑1 (MQ=255)

                                       cgtgcgtgGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTc                                                  <  1:4877751/93‑1 (MQ=255)

                                        gtgcgtgGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTcc                                                 <  2:889228/93‑1 (MQ=255)

                                        gtgcgtgGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTcc                                                 >  1:465413/1‑93 (MQ=255)

                                        gtgcgtgGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTcc                                                 <  2:451423/93‑1 (MQ=255)

                                        gtgcgtgGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTcc                                                 >  1:4623127/1‑93 (MQ=255)

                                          gcgtgGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCcgg                                               <  2:3459512/93‑1 (MQ=255)

                                          gcgtgGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCcgg                                               >  2:910122/1‑93 (MQ=255)

                                          gcgtgGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCcgg                                               <  1:1509959/93‑1 (MQ=255)

                                           cgtgGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCcggc                                              >  2:1533723/1‑93 (MQ=255)

                                                 acATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGaaaa                                        >  2:6089354/2‑93 (MQ=255)

                                                  cATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGaaaaa                                       >  2:1822924/1‑93 (MQ=255)

                                                    tGGAATTTATATCAGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGGACTTTTGCCGTTGATTCCGGCGGAAAAAtt                                     >  2:3967122/1‑93 (MQ=255)

                                                     ggAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGGAAAATTa                                    >  1:4805413/1‑93 (MQ=255)

                                                      gAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTAc                                   >  1:1891156/1‑93 (MQ=255)

                                                       aaTTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACt                                  <  1:2240952/93‑1 (MQ=255)

                                                        aTTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTg                                 <  1:1445552/93‑1 (MQ=255)

                                                         tttATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGa                                <  1:2207523/93‑1 (MQ=255)

                                                         tttATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGa                                >  1:2018430/1‑93 (MQ=255)

                                                          ttATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGAt                               <  1:1303035/93‑1 (MQ=255)

                                                          ttATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGAt                               <  2:1266007/93‑1 (MQ=255)

                                                           tataTCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATc                              <  1:3094648/93‑1 (MQ=255)

                                                           tataTCGGCATTACCGGTTGGGTTTGCGATG‑AGCGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATc                              <  1:2791283/93‑1 (MQ=255)

                                                             tatCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGa                            >  2:2027926/1‑93 (MQ=255)

                                                             tatCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGa                            >  1:995589/1‑93 (MQ=255)

                                                                cGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAAc                         <  2:2245992/93‑1 (MQ=255)

                                                                cGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAAc                         >  1:2643014/1‑93 (MQ=255)

                                                                   cATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGa                      <  1:2540190/93‑1 (MQ=255)

                                                                      tACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATgc                   >  1:4252472/1‑93 (MQ=255)

                                                                       aCCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATgcg                  >  2:5310166/1‑93 (MQ=255)

                                                                        ccGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGACACTGATgcgc                 >  2:676089/1‑93 (MQ=255)

                                                                        ccGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATgcgc                 >  2:5407011/1‑93 (MQ=255)

                                                                        ccGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATgcgc                 >  1:567837/1‑93 (MQ=255)

                                                                         cGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGcc                <  1:1034810/93‑1 (MQ=255)

                                                                         cGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGcc                <  2:2817574/93‑1 (MQ=255)

                                                                         cGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGcc                <  2:2553175/93‑1 (MQ=255)

                                                                          ggTTGGGTTTGCGATGAA‑CGACGCGGACTGGGGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCg               >  2:1699842/1‑93 (MQ=255)

                                                                          ggTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCg               >  1:2993188/1‑93 (MQ=255)

                                                                          ggTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCg               >  2:2870803/1‑93 (MQ=255)

                                                                           gTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGt              <  1:2084719/93‑1 (MQ=255)

                                                                            ttGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTa             <  2:4303654/93‑1 (MQ=255)

                                                                            ttGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTa             <  1:5012539/93‑1 (MQ=255)

                                                                              gggTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATc           <  1:1389656/93‑1 (MQ=255)

                                                                              gggTGTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATc           <  1:5938682/93‑1 (MQ=255)

                                                                               ggTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCt          <  1:3620445/93‑1 (MQ=255)

                                                                                    gCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTcc     >  1:1683066/1‑93 (MQ=255)

                                                                                     cGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTccc    <  1:6452519/93‑1 (MQ=255)

                                                                                      gATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTCCCt   >  2:3651473/1‑93 (MQ=255)

                                                                                      gATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTCCCt   <  1:5205129/93‑1 (MQ=255)

                                                                                      gATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTCCCt   <  1:2929504/93‑1 (MQ=255)

                                                                                       aTGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTCCCTc  >  2:5794773/1‑93 (MQ=255)

                                                                                       aTGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTCCCTc  >  2:213783/1‑93 (MQ=255)

                                                                                       aTGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTCCCTc  >  2:5279590/1‑93 (MQ=255)

                                                                                       aTGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTCCCTc  <  2:4667009/93‑1 (MQ=255)

                                                                                       aTGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTCCCTc  <  1:1173599/93‑1 (MQ=255)

                                                                                       aTGAA‑CGACGCGGACTGCAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTCCCTc  <  2:3050819/93‑1 (MQ=255)

TTCTTCATTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAACCGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTCCCTC  >  NC_000913/4021591‑4021771

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TTCTTCATTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAACCGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTCCCTCGCGATC  >  NC_000913/4021591‑4021777

TTCTTCATTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAACG                                                                                               <  SRR4302534.6519991/94‑1 (MQ=60)

TTCTTCATTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAACG                                                                                               <  SRR4302534.6130880/94‑1 (MQ=60)

  CTTCATTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGAC                                                                                            >  SRR4302534.6155049/1‑94 (MQ=60)

  CTTCATTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGAC                                                                                            <  SRR4302534.822725/94‑1 (MQ=60)

   TTCATTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACG                                                                                           <  SRR4302534.101054/94‑1 (MQ=60)

   TTCATTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACG                                                                                           >  SRR4302534.346135/1‑94 (MQ=60)

   TTCATTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACG                                                                                           >  SRR4302534.3531901/1‑94 (MQ=60)

    TCATTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGC                                                                                          >  SRR4302534.3193312/1‑94 (MQ=60)

    TCATTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGC                                                                                          >  SRR4302534.1838016/1‑94 (MQ=60)

     CATTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCG                                                                                         <  SRR4302534.5095462/94‑1 (MQ=60)

     CATTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCG                                                                                         <  SRR4302534.2328978/94‑1 (MQ=60)

      ATTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGG                                                                                        <  SRR4302534.3016915/94‑1 (MQ=60)

         GCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACT                                                                                     >  SRR4302534.1288275/1‑94 (MQ=60)

         GCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACT                                                                                     <  SRR4302534.3896104/94‑1 (MQ=60)

          CTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTG                                                                                    <  SRR4302534.4557051/94‑1 (MQ=60)

           TTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGG                                                                                   <  SRR4302534.5065688/94‑1 (MQ=60)

           TTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGG                                                                                   >  SRR4302534.3142173/1‑94 (MQ=60)

           TTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGG                                                                                   <  SRR4302534.6414393/94‑1 (MQ=60)

            TTACCGGCACACGCGAAGATATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGA                                                                                  <  SRR4302534.1162463/94‑1 (MQ=60)

            TTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGA                                                                                  <  SRR4302534.5243711/94‑1 (MQ=60)

            TTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGA                                                                                  <  SRR4302534.227247/94‑1 (MQ=60)

            TTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGA                                                                                  <  SRR4302534.1456189/94‑1 (MQ=60)

             TACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAG                                                                                 >  SRR4302534.5686274/1‑94 (MQ=60)

             TACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAG                                                                                 >  SRR4302534.2548053/1‑94 (MQ=60)

             TACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAG                                                                                 <  SRR4302534.5876754/94‑1 (MQ=60)

             TACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAG                                                                                 >  SRR4302534.133612/1‑94 (MQ=60)

               CCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCT                                                                               >  SRR4302534.2004971/1‑94 (MQ=60)

                CGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTG                                                                              <  SRR4302534.6214143/94‑1 (MQ=60)

                 GGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACCGGAGCTGC                                                                             >  SRR4302534.472528/1‑94 (MQ=60)

                  GCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCG                                                                            <  SRR4302534.708404/94‑1 (MQ=60)

                  GCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCG                                                                            <  SRR4302534.1979540/94‑1 (MQ=60)

                  GCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCG                                                                            >  SRR4302534.1426970/1‑94 (MQ=60)

                  GCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAAGTTATTTTGGCATTACCGGTTGGGGTTGCGATTAA‑CGACGCGGACTGGATCTGCG                                                                            >  SRR4302534.4599087/1‑94 (MQ=60)

                   CACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGG                                                                           <  SRR4302534.2732721/94‑1 (MQ=60)

                   CACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGG                                                                           >  SRR4302534.5842189/1‑94 (MQ=60)

                    ACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGG                                                                          >  SRR4302534.2173717/1‑94 (MQ=60)

                    ACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGG                                                                          <  SRR4302534.5540239/94‑1 (MQ=60)

                    ACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGG                                                                          >  SRR4302534.4314795/1‑94 (MQ=60)

                     CACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGA                                                                         >  SRR4302534.1577781/1‑94 (MQ=60)

                      ACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAA                                                                        >  SRR4302534.5618448/1‑94 (MQ=60)

                        GCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACT                                                                      >  SRR4302534.3355334/1‑94 (MQ=60)

                        GCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAAAT                                                                      <  SRR4302534.4624704/94‑1 (MQ=60)

                         CGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTT                                                                     <  SRR4302534.3850072/94‑1 (MQ=60)

                         CGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTT                                                                     <  SRR4302534.684134/94‑1 (MQ=60)

                         CGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTT                                                                     >  SRR4302534.946463/1‑94 (MQ=60)

                          GAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTT                                                                    >  SRR4302534.800169/1‑94 (MQ=60)

                          GAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTT                                                                    <  SRR4302534.3304516/94‑1 (MQ=60)

                          GAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTT                                                                    <  SRR4302534.2348198/94‑1 (MQ=60)

                            AGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTG                                                                  >  SRR4302534.6644839/1‑94 (MQ=60)

                            AGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTG                                                                  <  SRR4302534.785143/94‑1 (MQ=60)

                            AGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTG                                                                  >  SRR4302534.6423710/1‑94 (MQ=60)

                              AGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCC                                                                <  SRR4302534.3350122/94‑1 (MQ=60)

                              AGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCC                                                                >  SRR4302534.5712552/1‑94 (MQ=60)

                                 TGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTT                                                             <  SRR4302534.6227947/94‑1 (MQ=60)

                                 TGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTT                                                             <  SRR4302534.1754435/94‑1 (MQ=60)

                                  GCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTG                                                            <  SRR4302534.5934256/94‑1 (MQ=60)

                                  GCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTG                                                            <  SRR4302534.1515337/94‑1 (MQ=60)

                                  GCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTG                                                            >  SRR4302534.2937249/1‑94 (MQ=60)

                                   CAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGA                                                           >  SRR4302534.5055932/1‑94 (MQ=60)

                                   CAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGA                                                           >  SRR4302534.1632980/1‑94 (MQ=60)

                                    AGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGAT                                                          >  SRR4302534.1354267/1‑94 (MQ=60)

                                     GGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATT                                                         <  SRR4302534.4352132/94‑1 (MQ=60)

                                      GCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTC                                                        <  SRR4302534.3501563/94‑1 (MQ=60)

                                       CGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCC                                                       >  SRR4302534.4748569/1‑94 (MQ=60)

                                       CGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCC                                                       >  SRR4302534.477169/1‑94 (MQ=60)

                                       CGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCC                                                       <  SRR4302534.5010045/94‑1 (MQ=60)

                                        GTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCG                                                      <  SRR4302534.1748674/94‑1 (MQ=60)

                                        GTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCG                                                      >  SRR4302534.5292485/1‑94 (MQ=60)

                                        GTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCG                                                      <  SRR4302534.3089546/94‑1 (MQ=60)

                                         TGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGG                                                     <  SRR4302534.119848/94‑1 (MQ=60)

                                          GCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGC                                                    <  SRR4302534.1549659/94‑1 (MQ=60)

                                           CGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCG                                                   >  SRR4302534.611457/1‑94 (MQ=60)

                                           CGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCG                                                   <  SRR4302534.759686/94‑1 (MQ=60)

                                            GTGGCGCATGGAATGTATATCGGCATTACCGTTTGGGTTTGCTAT‑AAACGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTTATTCCGGCGG                                                  <  SRR4302534.1844463/94‑1 (MQ=60)

                                             TGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGA                                                 >  SRR4302534.1062177/1‑94 (MQ=60)

                                             TGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGA                                                 >  SRR4302534.1645695/1‑94 (MQ=60)

                                                   ATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATT                                           >  SRR4302534.2260512/1‑94 (MQ=60)

                                                   ATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATT                                           >  SRR4302534.3320995/1‑94 (MQ=60)

                                                    TGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGGAAAATTA                                          >  SRR4302534.4935884/1‑94 (MQ=60)

                                                     GGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTAC                                         >  SRR4302534.1940480/1‑94 (MQ=60)

                                                     GAAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTAC                                         <  SRR4302534.5795364/94‑1 (MQ=60)

                                                       AATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTG                                       <  SRR4302534.2300347/94‑1 (MQ=60)

                                                        ATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGA                                      <  SRR4302534.5780035/94‑1 (MQ=60)

                                                        ATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGA                                      >  SRR4302534.2071251/1‑94 (MQ=60)

                                                        ATTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGA                                      <  SRR4302534.1483604/94‑1 (MQ=60)

                                                        AATTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGA                                      <  SRR4302534.4287365/94‑1 (MQ=60)

                                                         TTTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGAT                                     <  SRR4302534.2265961/94‑1 (MQ=60)

                                                          TTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGCAATTTTGCCGATGATTCCGCAGGAAAACTTAATGATC                                    >  SRR4302534.3305407/1‑94 (MQ=60)

                                                          TTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATC                                    <  SRR4302534.4637272/94‑1 (MQ=60)

                                                          TTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATC                                    <  SRR4302534.1337405/94‑1 (MQ=60)

                                                          TTATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATC                                    <  SRR4302534.620067/94‑1 (MQ=60)

                                                           TATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCTGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCG                                   <  SRR4302534.2098815/94‑1 (MQ=60)

                                                           TATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCG                                   <  SRR4302534.3176054/94‑1 (MQ=60)

                                                           TATATCGGCATTACCGGTTGGGTTTGCGATG‑AGCGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCG                                   <  SRR4302534.2864697/94‑1 (MQ=60)

                                                            ATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGA                                  >  SRR4302534.806314/1‑94 (MQ=60)

                                                            ATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGA                                  >  SRR4302534.1021085/1‑94 (MQ=60)

                                                            ATATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGA                                  >  SRR4302534.4096681/1‑94 (MQ=60)

                                                             TATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAA                                 >  SRR4302534.5067955/1‑94 (MQ=60)

                                                             TATCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAA                                 <  SRR4302534.1935126/94‑1 (MQ=60)

                                                               TCGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAAC                               >  SRR4302534.2712910/1‑94 (MQ=60)

                                                                CGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACT                              <  SRR4302534.702776/94‑1 (MQ=60)

                                                                CGGCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACT                              <  SRR4302534.4865314/94‑1 (MQ=60)

                                                                  GCATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGA                            <  SRR4302534.248082/94‑1 (MQ=60)

                                                                   CATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGAT                           <  SRR4302534.2607588/94‑1 (MQ=60)

                                                                    ATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATG                          >  SRR4302534.3360969/1‑94 (MQ=60)

                                                                    ATTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATG                          >  SRR4302534.1854180/1‑94 (MQ=60)

                                                                     TTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGC                         >  SRR4302534.4367205/1‑94 (MQ=60)

                                                                     TTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGC                         >  SRR4302534.6622404/1‑94 (MQ=60)

                                                                     TTACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGC                         >  SRR4302534.2814411/1‑94 (MQ=60)

                                                                       ACCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGC                       >  SRR4302534.581775/1‑94 (MQ=60)

                                                                        CCGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCC                      <  SRR4302534.4766414/94‑1 (MQ=60)

                                                                         CGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCG                     <  SRR4302534.1061510/94‑1 (MQ=60)

                                                                         CGGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCG                     >  SRR4302534.3071412/1‑94 (MQ=60)

                                                                          GGTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGT                    >  SRR4302534.6120451/1‑94 (MQ=60)

                                                                           GTTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTA                   <  SRR4302534.2139342/94‑1 (MQ=60)

                                                                            TTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTAT                  <  SRR4302534.5148019/94‑1 (MQ=60)

                                                                            TTGGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTAT                  <  SRR4302534.4997898/94‑1 (MQ=60)

                                                                              GGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCT                <  SRR4302534.1426230/94‑1 (MQ=60)

                                                                              GGGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCT                >  SRR4302534.5297204/1‑94 (MQ=60)

                                                                              GGGTGTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCT                <  SRR4302534.6099934/94‑1 (MQ=60)

                                                                               GGTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTG               <  SRR4302534.3717776/94‑1 (MQ=60)

                                                                                GTTTGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGC              >  SRR4302534.5878910/1‑94 (MQ=60)

                                                                                   TGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTCC           >  SRR4302534.3581432/1‑94 (MQ=60)

                                                                                   TGCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTCC           >  SRR4302534.1726841/1‑94 (MQ=60)

                                                                                    GCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTCCC          <  SRR4302534.4866729/94‑1 (MQ=60)

                                                                                    GCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTCCC          <  SRR4302534.3710319/94‑1 (MQ=60)

                                                                                    GCGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTCCC          <  SRR4302534.5393852/94‑1 (MQ=60)

                                                                                     CGATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTCCCT         <  SRR4302534.6626391/94‑1 (MQ=60)

                                                                                      GATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTCCCTC        <  SRR4302534.5345573/94‑1 (MQ=60)

                                                                                      GATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTCCCTC        <  SRR4302534.3006423/94‑1 (MQ=60)

                                                                                       ATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTCCCTCG       >  SRR4302534.2349558/1‑94 (MQ=60)

                                                                                       ATGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTCCCTCG       <  SRR4302534.1204451/94‑1 (MQ=60)

                                                                                        TGAA‑CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTCCCTCGC      <  SRR4302534.4456367/94‑1 (MQ=60)

                                                                                             CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTCCCTCGCGATC  <  SRR4302534.673726/94‑1 (MQ=60)

                                                                                             CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTCCCTCGCGATC  >  SRR4302534.4737591/1‑94 (MQ=60)

                                                                                             CGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTCCCTCGCGATC  <  SRR4302534.147535/94‑1 (MQ=60)

TTCTTCATTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATATCGGCATTACCGGTTGGGTTTGCGATGAACCGACGCGGACTGGAGCTGCGGGAACTTTTGCCGTTGATTCCGGCGGAAAAATTACTGATCGAAACTGATGCGCCGTATCTGCTCCCTCGCGATC  >  NC_000913/4021591‑4021777

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`