Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 884 257.2 12958910 96.9% 12557183 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,057,859 (T)9→8 intergenic (+97/‑167) yihK → / → yihL putative GTP‑binding factor/putative transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,057,8510T.99.5% 980.3 / ‑2.6 210intergenic (+89/‑175)yihK/yihLputative GTP‑binding factor/putative transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base . (107/102);  total (107/103)
Fisher's exact test for biased strand distribution p-value = 4.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.08e-01

Only 100 of 245 total aligned reads displayed.

CTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATC  >  NC_000913/4057759‑4057941
                                                                                            |                                                                                          
cTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGt                                                                                            <  1:2832134/93‑1 (MQ=255)
 tGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGtt                                                                                           >  2:6391141/1‑93 (MQ=255)
  gACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGttt                                                                                          >  1:3597975/1‑93 (MQ=255)
   aCGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGtttt                                                                                         <  2:943754/93‑1 (MQ=255)
   aCGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGtttt                                                                                         >  1:5274884/1‑93 (MQ=255)
     ggAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGtttttt                                                                                       >  2:473073/1‑93 (MQ=255)
      gAAGACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGttttttt                                                                                      <  2:4450838/93‑1 (MQ=255)
      gAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGttttttt                                                                                      >  1:2595244/1‑93 (MQ=255)
       aaaaCGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGtttttttt                                                                                     >  1:2362376/1‑93 (MQ=255)
       aaaaCGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGtttttttt                                                                                     >  2:5589295/1‑93 (MQ=255)
        aaaCGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGttttttttg                                                                                    >  2:2876245/1‑92 (MQ=255)
          aCGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGttttttttgcc                                                                                  >  1:5416900/1‑90 (MQ=255)
          aCGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGttttttttgcc                                                                                  <  1:3693379/93‑4 (MQ=255)
          aCGAGCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTAAGTCATAAAAAACCTGCCAGCGATGGCAGGttttttttgcc                                                                                  <  1:4564225/93‑4 (MQ=255)
           cGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGttttttttgcct                                                                                 <  2:4155991/93‑5 (MQ=255)
           cGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGttttttttgcct                                                                                 <  2:3149961/93‑5 (MQ=255)
            gATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTg                                                                               >  2:2604512/1‑93 (MQ=255)
             aTCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGt                                                                              >  2:2008179/1‑93 (MQ=255)
              tcgtcgCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTc                                                                             >  2:618323/1‑93 (MQ=255)
              tcgtcgCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAAACTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGtt                                                                             >  1:3144110/1‑92 (MQ=255)
                gtcgCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGt                                                                           >  2:2410030/1‑93 (MQ=255)
                gtcgCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTAGCCTGTCGt                                                                           <  2:4769632/93‑1 (MQ=255)
                  cgccgcGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGa                                                                         >  2:1816421/1‑93 (MQ=255)
                    ccgcGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAgc                                                                       <  2:3988877/93‑1 (MQ=255)
                    ccgcGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAgc                                                                       >  1:901019/1‑93 (MQ=255)
                     cgcgCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAgcg                                                                      <  1:1002327/93‑1 (MQ=255)
                      gcgcCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAgcgc                                                                     >  1:4271954/1‑93 (MQ=255)
                       cgcCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGca                                                                    >  2:4956609/1‑93 (MQ=255)
                       cgcCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGca                                                                    <  2:1484024/93‑1 (MQ=255)
                        gcCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGcac                                                                   >  1:2991781/1‑93 (MQ=255)
                         ccAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGcaca                                                                  <  2:5797380/93‑1 (MQ=255)
                           aaCCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACAcc                                                                <  1:1909971/93‑1 (MQ=255)
                           aaCCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACAcc                                                                >  2:4948557/1‑93 (MQ=255)
                             ccGCGCCCCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCaa                                                              <  1:14265/93‑1 (MQ=255)
                             ccGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCaa                                                              <  2:5683985/93‑1 (MQ=255)
                                cgcACCAAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGc                                                           <  1:1344593/93‑1 (MQ=255)
                                 gcACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCg                                                          >  1:1636061/1‑93 (MQ=255)
                                 gcACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCg                                                          >  2:2779151/1‑93 (MQ=255)
                                  cACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGa                                                         <  1:3052341/93‑1 (MQ=255)
                                   aCCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAg                                                        >  1:4749402/1‑93 (MQ=255)
                                   aCCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAg                                                        >  1:5627384/1‑93 (MQ=255)
                                    ccGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGt                                                       <  2:2983961/93‑1 (MQ=255)
                                    ccGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGt                                                       >  2:1659068/1‑93 (MQ=255)
                                     cGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTa                                                      >  2:3389650/1‑93 (MQ=255)
                                     cGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTa                                                      >  2:4500216/1‑93 (MQ=255)
                                      gAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTAt                                                     >  1:1320406/1‑93 (MQ=255)
                                      gAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTAt                                                     >  1:3639525/1‑93 (MQ=255)
                                       aaaGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTAtc                                                    >  2:2197436/1‑93 (MQ=255)
                                       aaaGACAATTAATTTCGTTTTTTAGTCATAAAAAGCCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTAtc                                                    <  2:3655471/93‑1 (MQ=255)
                                          gACGATTAATTTCGTTTTTTAGTCTCAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTcc                                                 <  2:6243805/93‑1 (MQ=255)
                                           aCGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTccc                                                >  1:5870597/1‑93 (MQ=255)
                                            cGATTAATTTCGTTTTTTAGTCATAAAAACCCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCt                                               <  2:3312910/93‑1 (MQ=255)
                                            cGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCt                                               <  2:1047929/93‑1 (MQ=255)
                                             gATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCtt                                              >  2:3538822/1‑93 (MQ=255)
                                             gATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCtt                                              >  1:712449/1‑93 (MQ=255)
                                              aTTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTta                                             <  1:3914805/93‑1 (MQ=255)
                                               ttAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTtat                                            <  1:4909338/93‑1 (MQ=255)
                                                tattttCGTTTTTTAGTCATAAAAACCCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTtata                                           <  2:1203066/90‑1 (MQ=255)
                                                tAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTtata                                           <  1:5103481/93‑1 (MQ=255)
                                                 aaTTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATaa                                          <  1:6405921/93‑1 (MQ=255)
                                                  aTTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAg                                         <  1:6080516/93‑1 (MQ=255)
                                                    ttCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTc                                       <  1:4862598/93‑1 (MQ=255)
                                                     tCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCt                                      >  2:3703740/1‑93 (MQ=255)
                                                     tCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCt                                      >  1:4317268/1‑93 (MQ=255)
                                                     tCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCt                                      <  1:3769832/93‑1 (MQ=255)
                                                       gTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCtgt                                    <  1:5288302/93‑1 (MQ=255)
                                                        ttttttAGTCATAAAAACCCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCtgtg                                   <  2:5626230/93‑1 (MQ=255)
                                                        ttttttAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCtgtg                                   <  2:818640/93‑1 (MQ=255)
                                                         tttttAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTgg                                  <  2:1391617/93‑1 (MQ=255)
                                                          ttttAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGt                                 <  1:2419085/93‑1 (MQ=255)
                                                          ttttAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGt                                 <  1:3570339/93‑1 (MQ=255)
                                                             tAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTAc                              <  1:140599/93‑1 (MQ=255)
                                                              aGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACg                             >  2:3818890/1‑93 (MQ=255)
                                                                tCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTc                           <  1:201350/93‑1 (MQ=255)
                                                                tCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTc                           >  1:5829533/1‑93 (MQ=255)
                                                                 cATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCa                          <  1:1585627/93‑1 (MQ=255)
                                                                   tAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAAt                        <  1:4638279/93‑1 (MQ=255)
                                                                   tAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAAt                        <  1:4727642/93‑1 (MQ=255)
                                                                    aaaaaaCCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATa                       <  2:3562966/93‑1 (MQ=255)
                                                                     aaaaaCCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATag                      <  2:4352379/93‑1 (MQ=255)
                                                                      aaaaCCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATaga                     >  2:5966245/1‑93 (MQ=255)
                                                                       aaaCCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATagag                    >  1:951521/1‑93 (MQ=255)
                                                                       aaaCCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATagag                    >  2:4073935/1‑93 (MQ=255)
                                                                       aaaCCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATagag                    <  1:4029249/93‑1 (MQ=255)
                                                                        aaCCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATagaga                   >  2:1955185/1‑93 (MQ=255)
                                                                         aCCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATagagag                  >  2:3870343/1‑93 (MQ=255)
                                                                         aCCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATagagag                  >  2:27160/1‑93 (MQ=255)
                                                                         aCCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATagagag                  >  2:5408320/1‑93 (MQ=255)
                                                                         aCCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATagagag                  >  1:1783599/1‑93 (MQ=255)
                                                                           cTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCt                <  2:1256449/93‑1 (MQ=255)
                                                                           cTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCt                <  1:6109406/93‑1 (MQ=255)
                                                                           cTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCt                >  1:5601284/1‑93 (MQ=255)
                                                                           cTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCt                <  2:6328868/93‑1 (MQ=255)
                                                                            tGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCtt               <  2:525878/93‑1 (MQ=255)
                                                                            tGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCtt               >  1:3211905/1‑93 (MQ=255)
                                                                                aGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTc           >  2:4277491/1‑93 (MQ=255)
                                                                                 gCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTca          >  1:5716892/1‑93 (MQ=255)
                                                                                  cGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTcac         <  1:1117183/93‑1 (MQ=255)
                                                                                     tGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTTTCACAAt      >  1:1238405/1‑93 (MQ=255)
                                                                                          agTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATc  <  1:4468459/92‑1 (MQ=255)
                                                                                            |                                                                                          
CTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATC  >  NC_000913/4057759‑4057941

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGCAAAGTCAT  >  NC_000913/4057768‑4057952
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AACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCC                                                                                            <  SRR4302534.1588525/94‑1 (MQ=60)
AACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCC                                                                                            >  SRR4302534.5562606/1‑94 (MQ=60)
 ACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCT                                                                                           <  SRR4302534.3792687/94‑1 (MQ=60)
 ACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCT                                                                                           >  SRR4302534.4459365/1‑94 (MQ=60)
 ACGAGCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTAAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCT                                                                                           <  SRR4302534.4687968/94‑1 (MQ=60)
  CGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTG                                                                                          <  SRR4302534.3166700/94‑1 (MQ=60)
  CGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTG                                                                                          >  SRR4302534.1382053/1‑94 (MQ=60)
   GATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGT                                                                                         >  SRR4302534.2529974/1‑94 (MQ=60)
   GATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGT                                                                                         >  SRR4302534.1371210/1‑94 (MQ=60)
   GATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGT                                                                                         >  SRR4302534.5982935/1‑94 (MQ=60)
    ATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTC                                                                                        <  SRR4302534.6630729/94‑1 (MQ=60)
    ATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTC                                                                                        <  SRR4302534.413828/94‑1 (MQ=60)
     TCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCG                                                                                       <  SRR4302534.143318/94‑1 (MQ=60)
     TCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCG                                                                                       >  SRR4302534.5864753/1‑94 (MQ=60)
     TCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCG                                                                                       >  SRR4302534.3951043/1‑94 (MQ=60)
     ACGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAACCCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCG                                                                                       <  SRR4302534.5034678/94‑1 (MQ=60)
      CGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGT                                                                                      <  SRR4302534.3792040/94‑1 (MQ=60)
       GTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTG                                                                                     <  SRR4302534.1332765/94‑1 (MQ=60)
       GTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTG                                                                                     <  SRR4302534.4848219/94‑1 (MQ=60)
          GCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGC                                                                                  >  SRR4302534.923866/1‑94 (MQ=60)
          GCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGC                                                                                  >  SRR4302534.3374623/1‑94 (MQ=60)
            CGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGC                                                                                >  SRR4302534.4387465/1‑94 (MQ=60)
            CGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGC                                                                                <  SRR4302534.1028028/94‑1 (MQ=60)
            CGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑GTTTTTTTGCCTGTCGTGAGCGC                                                                                >  SRR4302534.3094729/1‑94 (MQ=60)
              CGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTTGTGAGCGCAA                                                                              >  SRR4302534.4916745/1‑94 (MQ=60)
              CGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCAC                                                                              >  SRR4302534.3069981/1‑94 (MQ=60)
               GCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACA                                                                             >  SRR4302534.862692/1‑94 (MQ=60)
                 CAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACC                                                                           >  SRR4302534.5596205/1‑94 (MQ=60)
                  AACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCA                                                                          <  SRR4302534.1959827/94‑1 (MQ=60)
                    CCGCGCCCCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAAT                                                                        <  SRR4302534.14644/94‑1 (MQ=60)
                     CGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATG                                                                       >  SRR4302534.1029377/1‑94 (MQ=60)
                     CGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATG                                                                       <  SRR4302534.2286973/94‑1 (MQ=60)
                       CGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCG                                                                     >  SRR4302534.1678709/1‑94 (MQ=60)
                       CGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCG                                                                     >  SRR4302534.3232985/1‑94 (MQ=60)
                       CGCACCGAAAGACGATTAATGTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCG                                                                     >  SRR4302534.6497263/1‑94 (MQ=60)
                       CGCACCAAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCG                                                                     <  SRR4302534.1380042/94‑1 (MQ=60)
                        GCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCTTGTCGTGGGCGCACACCAATGCGA                                                                    >  SRR4302534.4577633/1‑94 (MQ=60)
                        GCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGA                                                                    >  SRR4302534.2473970/1‑94 (MQ=60)
                         CACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAG                                                                   >  SRR4302534.5779676/1‑94 (MQ=60)
                         CACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAG                                                                   >  SRR4302534.4878316/1‑94 (MQ=60)
                         CACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAG                                                                   <  SRR4302534.382685/94‑1 (MQ=60)
                         CACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAG                                                                   <  SRR4302534.3132422/94‑1 (MQ=60)
                         CACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAAACTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAG                                                                   >  SRR4302534.4872138/1‑94 (MQ=60)
                            CGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTAT                                                                >  SRR4302534.1355225/1‑94 (MQ=60)
                            CGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTAT                                                                >  SRR4302534.3737336/1‑94 (MQ=60)
                              AAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCT                                                              <  SRR4302534.4744230/94‑1 (MQ=60)
                              AAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCT                                                              >  SRR4302534.2244950/1‑94 (MQ=60)
                                 GACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCC                                                           <  SRR4302534.961061/94‑1 (MQ=60)
                                 GACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCC                                                           >  SRR4302534.6029997/1‑94 (MQ=60)
                                   CGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTT                                                         >  SRR4302534.729964/1‑94 (MQ=60)
                                   CGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTT                                                         >  SRR4302534.4679321/1‑94 (MQ=60)
                                   CGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTT                                                         <  SRR4302534.1840174/94‑1 (MQ=60)
                                     ATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTAT                                                       <  SRR4302534.4019761/94‑1 (MQ=60)
                                      TTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATA                                                      >  SRR4302534.3182259/1‑94 (MQ=60)
                                      TTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATA                                                      <  SRR4302534.302635/94‑1 (MQ=60)
                                      TTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATA                                                      <  SRR4302534.5042401/94‑1 (MQ=60)
                                       TAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAA                                                     >  SRR4302534.2629005/1‑94 (MQ=60)
                                       TAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAA                                                     <  SRR4302534.5241224/94‑1 (MQ=60)
                                        AATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAG                                                    >  SRR4302534.5428885/1‑94 (MQ=60)
                                        AATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAG                                                    <  SRR4302534.6578835/94‑1 (MQ=60)
                                         ATTTCGTTTTTTAGTCATAAAAACCCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGT                                                   <  SRR4302534.5715917/94‑1 (MQ=60)
                                         ATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGT                                                   <  SRR4302534.6245890/94‑1 (MQ=60)
                                           TTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCT                                                 <  SRR4302534.4994513/94‑1 (MQ=60)
                                           TTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCT                                                 <  SRR4302534.3680141/94‑1 (MQ=60)
                                           TTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCT                                                 >  SRR4302534.4434169/1‑94 (MQ=60)
                                            TCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTG                                                <  SRR4302534.3702673/94‑1 (MQ=60)
                                            TCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTG                                                <  SRR4302534.4891435/94‑1 (MQ=60)
                                            TCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTG                                                <  SRR4302534.3871061/94‑1 (MQ=60)
                                            TCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTG                                                >  SRR4302534.1818593/1‑94 (MQ=60)
                                              GTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTG                                              <  SRR4302534.5431050/94‑1 (MQ=60)
                                              GTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTG                                              <  SRR4302534.1282551/94‑1 (MQ=60)
                                               TTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGG                                             <  SRR4302534.997776/94‑1 (MQ=60)
                                                TTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGT                                            >  SRR4302534.6028668/1‑94 (MQ=60)
                                                TTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGT                                            >  SRR4302534.5512837/1‑94 (MQ=60)
                                                 TTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTT                                           <  SRR4302534.904873/94‑1 (MQ=60)
                                                 TTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTT                                           <  SRR4302534.2483418/94‑1 (MQ=60)
                                                 TTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTT                                           <  SRR4302534.3666369/94‑1 (MQ=60)
                                                   TTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTAG                                         <  SRR4302534.1510605/94‑1 (MQ=60)
                                                    TAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACG                                        <  SRR4302534.144286/94‑1 (MQ=60)
                                                     AGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGCTTACGT                                       <  SRR4302534.4179795/94‑1 (MQ=60)
                                                      GTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTC                                      >  SRR4302534.5987769/1‑94 (MQ=60)
                                                       TCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCA                                     <  SRR4302534.206640/94‑1 (MQ=60)
                                                        CATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAA                                    <  SRR4302534.1627105/94‑1 (MQ=60)
                                                          TAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATA                                  <  SRR4302534.4764131/94‑1 (MQ=60)
                                                          TAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATA                                  <  SRR4302534.4856039/94‑1 (MQ=60)
                                                           AAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAG                                 <  SRR4302534.5774387/94‑1 (MQ=60)
                                                           AAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAG                                 <  SRR4302534.1744741/94‑1 (MQ=60)
                                                            AAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGA                                >  SRR4302534.3050786/1‑94 (MQ=60)
                                                            AAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGA                                >  SRR4302534.4050580/1‑94 (MQ=60)
                                                            AAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGA                                <  SRR4302534.6262457/94‑1 (MQ=60)
                                                             AAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAG                               >  SRR4302534.975756/1‑94 (MQ=60)
                                                              aaacctttccatcgatggtatgTTTTTGTTGCCTGTCGTGAGCGCACACCAAGGCGAGTATCTCCCTTATAGGTCTGTGGTTACGCCAATAGAG                               <  SRR4302534.3862757/72‑1 (MQ=60)
                                                              AAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGA                              <  SRR4302534.5074738/94‑1 (MQ=60)
                                                              AAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGA                              <  SRR4302534.4137275/94‑1 (MQ=60)
                                                              AAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGA                              <  SRR4302534.3758640/94‑1 (MQ=60)
                                                              AAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGA                              >  SRR4302534.3634007/1‑94 (MQ=60)
                                                               AACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAG                             >  SRR4302534.1829916/1‑94 (MQ=60)
                                                                 CCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCT                           >  SRR4302534.5752764/1‑94 (MQ=60)
                                                                  CTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTT                          <  SRR4302534.6275558/94‑1 (MQ=60)
                                                                  CTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTT                          >  SRR4302534.3297133/1‑94 (MQ=60)
                                                                   TGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTT                         <  SRR4302534.5072229/94‑1 (MQ=60)
                                                                    GCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTG                        <  SRR4302534.2038705/94‑1 (MQ=60)
                                                                     CCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGT                       <  SRR4302534.3889321/94‑1 (MQ=60)
                                                                       AGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCA                     >  SRR4302534.5871783/1‑94 (MQ=60)
                                                                       AGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCA                     <  SRR4302534.6482160/94‑1 (MQ=60)
                                                                       AGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCA                     <  SRR4302534.5428406/94‑1 (MQ=60)
                                                                        GCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCAC                    >  SRR4302534.1661565/1‑94 (MQ=60)
                                                                         CGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACA                   >  SRR4302534.1255173/1‑94 (MQ=60)
                                                                         CGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACA                   >  SRR4302534.5281530/1‑94 (MQ=60)
                                                                         CGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACA                   <  SRR4302534.1146358/94‑1 (MQ=60)
                                                                          GATGGCAGG‑ATTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAA                  >  SRR4302534.6138968/1‑94 (MQ=60)
                                                                           ATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTTTCACAAT                 >  SRR4302534.1271047/1‑94 (MQ=60)
                                                                               CAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATC             <  SRR4302534.262265/94‑1 (MQ=60)
                                                                                AGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCT            <  SRR4302534.5633575/94‑1 (MQ=60)
                                                                                AGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCT            <  SRR4302534.5536109/94‑1 (MQ=60)
                                                                                AGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCT            <  SRR4302534.3560108/94‑1 (MQ=60)
                                                                                AGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCT            <  SRR4302534.353123/94‑1 (MQ=60)
                                                                                AGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCT            >  SRR4302534.1938234/1‑94 (MQ=60)
                                                                                AGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCT            <  SRR4302534.2748907/94‑1 (MQ=60)
                                                                                 AGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCT            <  SRR4302534.4589304/94‑1 (MQ=60)
                                                                                  GGTTTTTTTTGCCTGTCGTGAGCGCTCATCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTG           <  SRR4302534.1988776/94‑1 (MQ=60)
                                                                                  GGTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTG           >  SRR4302534.2114687/1‑94 (MQ=60)
                                                                                   GTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGC          <  SRR4302534.2347740/94‑1 (MQ=60)
                                                                                   GTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGC          >  SRR4302534.6333964/1‑94 (MQ=60)
                                                                                   GTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGC          <  SRR4302534.4728826/94‑1 (MQ=60)
                                                                                    TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTCCA         <  SRR4302534.3437929/94‑1 (MQ=60)
                                                                                     TTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGCAA        >  SRR4302534.4550270/1‑94 (MQ=60)
                                                                                       TTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGCAAAG      <  SRR4302534.5994397/94‑1 (MQ=60)
                                                                                       TTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGCAAAG      <  SRR4302534.3086023/94‑1 (MQ=60)
                                                                                       TTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGCAAAG      <  SRR4302534.3780291/94‑1 (MQ=60)
                                                                                       TTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCAGCAAAG      <  SRR4302534.4299992/94‑1 (MQ=60)
                                                                                        TTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGCAAAGT     <  SRR4302534.841830/94‑1 (MQ=60)
                                                                                           TGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGCAAAGTCAT  >  SRR4302534.2525176/1‑94 (MQ=60)
                                                                                           TGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGCAAAGTCAT  >  SRR4302534.2843212/1‑94 (MQ=60)
                                                                                           TGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGCAAAGTCAT  <  SRR4302534.98074/94‑1 (MQ=60)
                                                                                           |                                                                                             
AACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGCAAAGTCAT  >  NC_000913/4057768‑4057952

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑