Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 884 257.2 12958910 96.9% 12557183 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,071,652 A→G S114G (AGC→GGC)  yihV → putative kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,071,6520AG99.6% 822.2 / ‑4.6 241S114G (AGC→GGC) yihVputative kinase
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (103/137);  total (103/138)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.18e-01

Only 100 of 251 total aligned reads displayed.

CGTTACACCAAACGGTATAACCAGGCGAAATCTTCGCAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCAGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTACGCTGGCACGAC  >  NC_000913/4071562‑4071741
                                                                                          |                                                                                         
cGTTACACCAAACGGTATAACCAGGCGAAATCTTCGCAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGc                                                                                         <  1:2217072/93‑1 (MQ=255)
 gTTACACCAAACGGTATAACCAGGCGAAATCTTCGCAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGcc                                                                                        <  2:3655604/93‑1 (MQ=255)
 gTTACACCAAACGGTATAACCAGGCGAAATCTTCGCAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGcc                                                                                        <  1:4386416/93‑1 (MQ=255)
   tACACCAAACGGTATAACCAGGCGAAATCTTCGCAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCg                                                                                      >  1:1734338/1‑93 (MQ=255)
     cacCAAACGGTATAACCAGGCGAAATCTTCGCAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGa                                                                                    >  2:862899/1‑93 (MQ=255)
     cacCAAACGGTATAACCAGGCGAAATCTTCGCAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGa                                                                                    <  2:813888/93‑1 (MQ=255)
     cacCAAACGGTATAACCAGGCGAAATCTTCGCAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGa                                                                                    <  2:1278085/93‑1 (MQ=255)
        cAAACGGTATAACCAGGCGAAATCTTCGCAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATct                                                                                 >  2:3331370/1‑93 (MQ=255)
          aaCGGTATAACCAGGCGAAATCTTCGCAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATctgc                                                                               <  1:1097760/93‑1 (MQ=255)
          aaCGGTATAACCAGGCGAAATCTTCGCAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATctgc                                                                               <  2:376055/93‑1 (MQ=255)
          aaCGGTATAACCAGGCGAAATCTTCGCAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATctgc                                                                               >  2:571104/1‑93 (MQ=255)
          aaCGGTATAACCAGGCGAAATCTTCGCAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCAGCCCGGATctgc                                                                               <  2:3082150/93‑1 (MQ=255)
           aCGGTATAACCAGGCGAAATCTTCGCAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATctgct                                                                              >  2:4630256/1‑93 (MQ=255)
            cGGTATAACCAGGCGAAATCTTCGCAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATctgctg                                                                             >  1:624473/1‑93 (MQ=255)
              gTATAACCAGGCGAAATCTTCGCAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGcc                                                                           >  2:4055755/1‑93 (MQ=255)
              gTATAACCAGGCGAAATCTTCGCAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGcc                                                                           <  2:3426619/93‑1 (MQ=255)
                ataACCAGGCGAAATCTTCGCAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTg                                                                         <  1:4683339/93‑1 (MQ=255)
                 taACCAGGCGAAATCTTCGCAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGa                                                                        <  2:786026/93‑1 (MQ=255)
                  aaCCAGGCGAAATCTTCGCAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGAc                                                                       <  2:5847199/93‑1 (MQ=255)
                  aaCCAGGCGAAATCTTCGCAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGAc                                                                       >  1:5411517/1‑93 (MQ=255)
                   aCCAGGCGAAATCTTCGCAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACg                                                                      >  2:6167785/1‑93 (MQ=255)
                    ccAGGCGAAATCTTCGCAATCCGCCAGCATGGTGGAAACCAAAGGGGAGCGGATAATCATTAACTCCCCCGGCCCAGATCTGCTGCCTGACGc                                                                     <  2:1051626/93‑1 (MQ=255)
                       ggCGAAATCTTCGCAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCaga                                                                  >  1:6464199/1‑93 (MQ=255)
                        gCGAAATCTTCGCAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCagag                                                                 <  1:5879693/93‑1 (MQ=255)
                        gCGAAATCTTCGCAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCagag                                                                 <  2:4731588/93‑1 (MQ=255)
                         cGAAATCTTCGCAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGt                                                                <  2:5054346/93‑1 (MQ=255)
                          gAAATCTTCGCAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCagaggg                                                               >  2:1298791/1‑91 (MQ=255)
                           aaaTCTTCGCAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTgg                                                              >  2:1774133/1‑93 (MQ=255)
                           aaaTCTTCGCAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGCgg                                                              >  1:3198895/1‑93 (MQ=255)
                            aaTCTTCGCAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGt                                                             <  1:1528092/93‑1 (MQ=255)
                            aaTCTTCGCAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGt                                                             >  1:6018247/1‑93 (MQ=255)
                             aTCTTCGCAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGtt                                                            >  1:1937954/1‑93 (MQ=255)
                             aTCTTCGCAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGtt                                                            >  1:2067826/1‑93 (MQ=255)
                             aTCTTCGCAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGtt                                                            <  2:4858927/93‑1 (MQ=255)
                             aTCTTCGCAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGtt                                                            <  2:1909611/93‑1 (MQ=255)
                             aTCTTCGCAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGtt                                                            <  2:5553772/93‑1 (MQ=255)
                             aTCTTCGCAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGCGGtt                                                            >  2:5174609/1‑93 (MQ=255)
                              tCTTCGCAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTg                                                           >  1:4158213/1‑93 (MQ=255)
                              tCTTCGCAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTg                                                           >  2:5645704/1‑93 (MQ=255)
                               cTTCGCAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTgg                                                          <  1:163611/93‑1 (MQ=255)
                                ttCGCAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTgga                                                         <  1:6068810/93‑1 (MQ=255)
                                    cAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGaa                                                     >  1:5713325/1‑93 (MQ=255)
                                    cAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGaa                                                     >  2:347143/1‑93 (MQ=255)
                                    cAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGaa                                                     >  2:6222568/1‑93 (MQ=255)
                                     aaTCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGaaa                                                    <  2:1642330/93‑1 (MQ=255)
                                     aaTCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGaaa                                                    <  2:1763393/93‑1 (MQ=255)
                                      aTCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAAt                                                   >  2:4354827/1‑93 (MQ=255)
                                       tCCGCCATCATGGTGGATACCAAAGGCGAGCGTATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAAtt                                                  <  2:6313559/93‑1 (MQ=255)
                                       tCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAAtt                                                  >  2:6092608/1‑93 (MQ=255)
                                       tCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAAtt                                                  <  2:124861/93‑1 (MQ=255)
                                          gCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGAt                                               >  2:3341542/1‑93 (MQ=255)
                                            catcatGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGAttt                                             >  1:3849964/1‑93 (MQ=255)
                                            catcatGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGAttt                                             <  1:2869852/93‑1 (MQ=255)
                                             atcatGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGCGGTTGGAGGAAATTGATTtc                                            >  1:4878807/1‑93 (MQ=255)
                                              tcatGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTtct                                           >  2:2203008/1‑93 (MQ=255)
                                                 tggtggATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTtctctc                                        >  2:4102372/1‑93 (MQ=255)
                                                  ggtggATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCa                                       <  2:3126863/93‑1 (MQ=255)
                                                  ggtggATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCa                                       <  1:1351628/93‑1 (MQ=255)
                                                  ggtggATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCa                                       >  1:1856333/1‑93 (MQ=255)
                                                    tggATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGt                                     <  1:3575657/93‑1 (MQ=255)
                                                      gATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTgg                                   <  2:4954449/93‑1 (MQ=255)
                                                      gATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTgg                                   >  2:2981556/1‑93 (MQ=255)
                                                       aTACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTggg                                  >  1:4803784/1‑93 (MQ=255)
                                                        tACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCCGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGa                                 >  2:1542591/1‑93 (MQ=255)
                                                         aCCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGAt                                <  1:4933533/93‑1 (MQ=255)
                                                         aCCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGAt                                <  2:5478895/93‑1 (MQ=255)
                                                         aCCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGAt                                >  2:5220434/1‑93 (MQ=255)
                                                          ccAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATCGATTTCTCTCAGTGGGAtg                               <  1:6327177/93‑1 (MQ=255)
                                                           cAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGAtgt                              <  2:6276722/93‑1 (MQ=255)
                                                            aaaGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGAtgtt                             >  2:4566104/1‑93 (MQ=255)
                                                            aaaGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGAtgtt                             <  1:2379868/93‑1 (MQ=255)
                                                              aGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGAtgttgt                           <  1:2487570/93‑1 (MQ=255)
                                                              aGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGAtgttgt                           >  1:5829902/1‑93 (MQ=255)
                                                              aGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGAtgttgt                           >  2:3044304/1‑93 (MQ=255)
                                                               ggCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTtgtg                          <  2:2517287/93‑1 (MQ=255)
                                                                gCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGc                         <  2:238772/93‑1 (MQ=255)
                                                                 cGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCt                        <  1:3649045/93‑1 (MQ=255)
                                                                 cGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCt                        >  1:5034611/1‑93 (MQ=255)
                                                                 cGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCt                        <  1:208826/93‑1 (MQ=255)
                                                                  gAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTg                       >  1:2812341/1‑93 (MQ=255)
                                                                      ggATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAg                   >  1:4195631/1‑93 (MQ=255)
                                                                       gATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGa                  >  2:2739347/1‑93 (MQ=255)
                                                                        aTAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTCGTTGGAGGAAATTGATTTCTCTTAGTGGGATGTTGTGCTGGCAGAt                 <  1:5181264/93‑1 (MQ=255)
                                                                          aaTCATTAACTGCCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGt               <  2:1403155/93‑1 (MQ=255)
                                                                           agcATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTa              <  1:613524/91‑1 (MQ=255)
                                                                           aTCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTa              <  2:200069/93‑1 (MQ=255)
                                                                            tCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTAc             >  2:6019101/1‑93 (MQ=255)
                                                                            tCATTAACTACCCCGCACCGGATCTGCTGCCTGACGCAGAGTGGTTGCAGGAAATTGATTTCTCTCAGTCGGATGTTGTGCTGGCAGAAGTAc             >  1:6377265/1‑93 (MQ=255)
                                                                             cATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTACg            <  1:1410854/93‑1 (MQ=255)
                                                                              aTTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTACGc           >  1:446028/1‑93 (MQ=255)
                                                                              aTTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTACGc           <  2:2092352/93‑1 (MQ=255)
                                                                              aTTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATCTCTCTCAGTGGGCTGTTGTGCTGGCAGATGTACGc           >  1:3767941/1‑93 (MQ=255)
                                                                               ttAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTACGCt          <  2:5598079/93‑1 (MQ=255)
                                                                               ttAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTACGCt          <  1:751098/93‑1 (MQ=255)
                                                                               ttAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTACGCt          <  2:2628810/93‑1 (MQ=255)
                                                                                  aCTACCCCGGCTTGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTACGCTGGc       <  1:3051163/93‑1 (MQ=255)
                                                                                  aCTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTACGCTGGc       <  1:2588764/93‑1 (MQ=255)
                                                                                     aCCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTACGCTGGCacg    >  1:3304420/1‑93 (MQ=255)
                                                                                       cccggcccggATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTACGCTGGCacgac  <  1:2922144/93‑1 (MQ=255)
                                                                                       cccggcccggATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGCACGCTGGCacgac  >  2:4153647/1‑93 (MQ=255)
                                                                                          |                                                                                         
CGTTACACCAAACGGTATAACCAGGCGAAATCTTCGCAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCAGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTACGCTGGCACGAC  >  NC_000913/4071562‑4071741

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ACCCGTTACACCAAACGGTATAACCAGGCGAAATCTTCGCAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCAGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTACGCTGGCACGACGGCG  >  NC_000913/4071559‑4071745
                                                                                             |                                                                                             
ACCCGTTACACCAAACGGTATAACCAGGCGAAATCTTCGCAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCG                                                                                               <  SRR4302534.372587/94‑1 (MQ=60)
 CCCGTTACACCAAACGGTATAACAAGGCGAAATCTTCGCAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGG                                                                                              <  SRR4302534.2842560/94‑1 (MQ=60)
 CCCGTTACAACAAACGGTATCACCAGGCGAAATCTTCGCAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGG                                                                                              >  SRR4302534.6476578/1‑94 (MQ=60)
  CCGTTACACCAAACGGTATAACCAGGCGAAATCTTCGCAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGC                                                                                             >  SRR4302534.3585814/1‑94 (MQ=60)
   CGTTACACCAAACGGTATAACCAGGCGAAATCTTCGCAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCC                                                                                            <  SRR4302534.2275783/94‑1 (MQ=60)
    GTTACACCAAACGGTATAACCAGGCGAAATCTTCGCAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCC                                                                                           <  SRR4302534.4504684/94‑1 (MQ=60)
     TTACACCAAACGGTATAACCAGGCGAAATCTTCGCAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCG                                                                                          <  SRR4302534.1228877/94‑1 (MQ=60)
     TTACACCAAACGGTATAACCAGGCGAAATCTTCGCAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCG                                                                                          >  SRR4302534.1779381/1‑94 (MQ=60)
          aaaaacggtaaaacgagtcgaaatcttgatAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTACCTACCCCGGCCCGGATCT                                                                                     <  SRR4302534.3809283/64‑1 (MQ=60)
          CCAAACGGTATAACCAGGCGAAATCTTCGCAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCT                                                                                     <  SRR4302534.1651573/94‑1 (MQ=60)
            AAACGGTATAACCAGGCGAAATCTTCGCAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGC                                                                                   >  SRR4302534.4352732/1‑94 (MQ=60)
             AACGGTATAACCAGGCGAAATCTTCGCAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCT                                                                                  <  SRR4302534.1126337/94‑1 (MQ=60)
              ACGGTATAACCAGGCGAAATCTTCGCAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTG                                                                                 >  SRR4302534.5249621/1‑94 (MQ=60)
              ACGGTATAACCAGGCGAAATCTTCGCAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTG                                                                                 >  SRR4302534.639624/1‑94 (MQ=60)
                GGTATAACCAGGCGAAATCTTCGCAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCC                                                                               >  SRR4302534.5461000/1‑94 (MQ=60)
                 GTATAACCAGGCGAAATCTTCGCAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCT                                                                              <  SRR4302534.6287490/94‑1 (MQ=60)
                  TAAAACCAGTCTAAATCTTCGCAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTG                                                                             <  SRR4302534.3355965/94‑1 (MQ=60)
                   ATAACCAGGCGAAATCTTCGCAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGA                                                                            <  SRR4302534.4810488/94‑1 (MQ=60)
                    TAACCAGGCGAAATCTTCGCAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGAC                                                                           <  SRR4302534.4014381/94‑1 (MQ=60)
                    TAACCAGGCGAAATCTTCGCAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGAC                                                                           >  SRR4302534.5557100/1‑94 (MQ=60)
                     AACCAGGCGAAATCTTCGCAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACG                                                                          <  SRR4302534.245192/94‑1 (MQ=60)
                        CAGGCGAAATCTTCGCAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAG                                                                       <  SRR4302534.6004090/94‑1 (MQ=60)
                        CAGGCGAAATCTTCGCAATCCGCCATCATGGTGGATACCAAAGACGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAG                                                                       >  SRR4302534.2148811/1‑94 (MQ=60)
                         AGGCGAAATCTTCGCAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGA                                                                      >  SRR4302534.6486862/1‑94 (MQ=60)
                         AGGCGAAATCTTCGCAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGA                                                                      >  SRR4302534.213816/1‑94 (MQ=60)
                         AGGCGAAATCTTCGCAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGA                                                                      >  SRR4302534.6638306/1‑94 (MQ=60)
                           GCGAAATCTTCGCAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGT                                                                    <  SRR4302534.6039334/94‑1 (MQ=60)
                            CGAAATCTTCGCAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTG                                                                   <  SRR4302534.6139472/94‑1 (MQ=60)
                             GAAATCTTCGCAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGG                                                                  >  SRR4302534.4922689/1‑94 (MQ=60)
                             GAAATCTTCGCAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGCGG                                                                  >  SRR4302534.3283706/1‑94 (MQ=60)
                              AAATCTTCGCAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGT                                                                 >  SRR4302534.85205/1‑94 (MQ=60)
                              AAATCTTCGCAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGT                                                                 >  SRR4302534.6181774/1‑94 (MQ=60)
                               AATCTTCGCAATCCGCCATCATGGTGGATGCCAAAGCCGAGCGGATAATCATGACCTCCCCCGGCCCGGATCTGCTCCCTGACGCAGAGTGGTT                                                                <  SRR4302534.4558646/94‑1 (MQ=60)
                               AATCTTCGCAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTT                                                                <  SRR4302534.5572802/94‑1 (MQ=60)
                               AATCTTCGCAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTT                                                                <  SRR4302534.1568200/94‑1 (MQ=60)
                               AATCTTCGCAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTT                                                                >  SRR4302534.1988628/1‑94 (MQ=60)
                               AATCTTCGCAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTT                                                                >  SRR4302534.2121905/1‑94 (MQ=60)
                                ATCTTCGCAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTG                                                               <  SRR4302534.1762651/94‑1 (MQ=60)
                                ATCTTCGCAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTG                                                               >  SRR4302534.4270502/1‑94 (MQ=60)
                                ATCTTCGCAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTG                                                               <  SRR4302534.4696220/94‑1 (MQ=60)
                                  CTTCGCAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGG                                                             >  SRR4302534.6582453/1‑94 (MQ=60)
                                  CTTCGCAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGG                                                             >  SRR4302534.4565707/1‑94 (MQ=60)
                                  CTTCGCAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGA                                                             <  SRR4302534.167895/94‑1 (MQ=60)
                                   TTCGCAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAG                                                            <  SRR4302534.6511667/94‑1 (MQ=60)
                                   TTCGCAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAG                                                            <  SRR4302534.6256507/94‑1 (MQ=60)
                                   TTCGCAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAG                                                            <  SRR4302534.6233803/94‑1 (MQ=60)
                                   TTCGCAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAG                                                            >  SRR4302534.2987671/1‑94 (MQ=60)
                                    TCGCAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGG                                                           <  SRR4302534.3819797/94‑1 (MQ=60)
                                     CGCAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGA                                                          >  SRR4302534.2285737/1‑94 (MQ=60)
                                     CGCAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGA                                                          >  SRR4302534.2884692/1‑94 (MQ=60)
                                      GCAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAA                                                         >  SRR4302534.5868108/1‑94 (MQ=60)
                                       CAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAA                                                        >  SRR4302534.2052801/1‑94 (MQ=60)
                                        AATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAAT                                                       >  SRR4302534.4432037/1‑94 (MQ=60)
                                        AATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAAT                                                       >  SRR4302534.5147507/1‑94 (MQ=60)
                                        AATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAAT                                                       <  SRR4302534.4600216/94‑1 (MQ=60)
                                              CCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTT                                                 >  SRR4302534.3953343/1‑94 (MQ=60)
                                               CATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTC                                                <  SRR4302534.2945212/94‑1 (MQ=60)
                                               CATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGCGGTTGGAGGAAATTGATTTC                                                >  SRR4302534.5011127/1‑94 (MQ=60)
                                                ATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCT                                               >  SRR4302534.2082370/1‑94 (MQ=60)
                                                   ATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTC                                            <  SRR4302534.4956374/94‑1 (MQ=60)
                                                    TGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCA                                           <  SRR4302534.1432008/94‑1 (MQ=60)
                                                    TGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCA                                           <  SRR4302534.5002801/94‑1 (MQ=60)
                                                    TGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCA                                           >  SRR4302534.1904643/1‑94 (MQ=60)
                                                     GGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAG                                          <  SRR4302534.1387262/94‑1 (MQ=60)
                                                       TGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTG                                        <  SRR4302534.3671828/94‑1 (MQ=60)
                                                         GATACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGG                                      >  SRR4302534.4934218/1‑94 (MQ=60)
                                                            ACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATG                                   <  SRR4302534.5067231/94‑1 (MQ=60)
                                                            ACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATG                                   <  SRR4302534.5033257/94‑1 (MQ=60)
                                                            ACCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATG                                   <  SRR4302534.368443/94‑1 (MQ=60)
                                                             CCAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATCGATTTCTCTCAGTGGGATGT                                  <  SRR4302534.6498115/94‑1 (MQ=60)
                                                              CAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTT                                 >  SRR4302534.6337107/1‑94 (MQ=60)
                                                              CAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTT                                 <  SRR4302534.6216569/94‑1 (MQ=60)
                                                              CAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTT                                 <  SRR4302534.591998/94‑1 (MQ=60)
                                                              CAAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTT                                 <  SRR4302534.6457393/94‑1 (MQ=60)
                                                               AAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTG                                >  SRR4302534.1808321/1‑94 (MQ=60)
                                                               AAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTG                                <  SRR4302534.2443100/94‑1 (MQ=60)
                                                               AAAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTG                                <  SRR4302534.2990959/94‑1 (MQ=60)
                                                                AAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTTATTTCTCTCAGTGGGATGTTGT                               >  SRR4302534.3160542/1‑94 (MQ=60)
                                                                AAGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGT                               >  SRR4302534.5988149/1‑94 (MQ=60)
                                                                 AGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTG                              <  SRR4302534.1655760/94‑1 (MQ=60)
                                                                 AGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTG                              <  SRR4302534.3756647/94‑1 (MQ=60)
                                                                 AGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTG                              >  SRR4302534.1543080/1‑94 (MQ=60)
                                                                 AGGCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTG                              <  SRR4302534.2553652/94‑1 (MQ=60)
                                                                   GCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCT                            >  SRR4302534.5170601/1‑94 (MQ=60)
                                                                   GCGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCT                            <  SRR4302534.4271723/94‑1 (MQ=60)
                                                                    CGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTG                           <  SRR4302534.3747103/94‑1 (MQ=60)
                                                                    CGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTG                           <  SRR4302534.1925732/94‑1 (MQ=60)
                                                                    CGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTG                           <  SRR4302534.5097734/94‑1 (MQ=60)
                                                                    CGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTG                           <  SRR4302534.214318/94‑1 (MQ=60)
                                                                    CGAGCGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTG                           >  SRR4302534.2886277/1‑94 (MQ=60)
                                                                        CGGATTATCATTAACTACCCCGGCCCGGTTCTGCTGCCTGACGCAGAGCGGTTGGATGAAATTGATTTCTCTCAGTGCGATGGTGTGCTGGCAG                       >  SRR4302534.6647507/1‑94 (MQ=60)
                                                                        CGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCCGAGTGGTTGGAGGACATTGGTTTCTCTCCGTGGGAAGTTGTGCTGGCAG                       >  SRR4302534.271891/1‑94 (MQ=60)
                                                                        CGGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAG                       >  SRR4302534.4308970/1‑94 (MQ=60)
                                                                         GGATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTCGCAGA                      <  SRR4302534.356112/94‑1 (MQ=60)
                                                                           ATAATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTCGTTGGAGGAAATTGATTTCTCTTAGTGGGATGTTGTGCTGGCAGATG                    <  SRR4302534.5320903/94‑1 (MQ=60)
                                                                              ATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTAC                 <  SRR4302534.6620622/94‑1 (MQ=60)
                                                                              ATCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTAC                 <  SRR4302534.1441537/94‑1 (MQ=60)
                                                                              ATCATTAACTACCCCGCACCGGATCTGCTGCCTGACGCAGAGTGGTTGCAGGAAATTGATTTCTCTCAGTCGGATGTTGTGCTGGCAGAAGTAC                 >  SRR4302534.6549515/1‑94 (MQ=60)
                                                                              AGCATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTAC                 <  SRR4302534.628425/94‑1 (MQ=60)
                                                                                CATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTACGC               <  SRR4302534.3252507/94‑1 (MQ=60)
                                                                                CATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTACGC               <  SRR4302534.1447969/94‑1 (MQ=60)
                                                                                CATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTACGC               >  SRR4302534.457341/1‑94 (MQ=60)
                                                                                CATTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATCTCTCTCAGTGGGCTGTTGTGCTGGCAGATGTACGC               >  SRR4302534.3869098/1‑94 (MQ=60)
                                                                                  TTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTACGCTG             <  SRR4302534.769709/94‑1 (MQ=60)
                                                                                  TTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTACGCTG             <  SRR4302534.886325/94‑1 (MQ=60)
                                                                                  TTAACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTACGCTG             <  SRR4302534.2595193/94‑1 (MQ=60)
                                                                                     ACTACCCCGGCTTGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTACGCTGGCA          <  SRR4302534.3131212/94‑1 (MQ=60)
                                                                                     ACTACCCCGGCCCGTATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTACGCTGGCA          <  SRR4302534.2929820/94‑1 (MQ=60)
                                                                                     ACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTACGCTGGCA          <  SRR4302534.5880555/94‑1 (MQ=60)
                                                                                     ACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTACGCTGGCA          >  SRR4302534.1826133/1‑94 (MQ=60)
                                                                                     ACTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTACGCTGGCA          <  SRR4302534.2657292/94‑1 (MQ=60)
                                                                                      CTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTACGCTGGCAC         >  SRR4302534.4070501/1‑94 (MQ=60)
                                                                                      CTACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTACGCTGGCAC         >  SRR4302534.3060326/1‑94 (MQ=60)
                                                                                       TACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTACGCTGGCACG        >  SRR4302534.3392625/1‑94 (MQ=60)
                                                                                       TACCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTACGCTGGCACG        >  SRR4302534.5485651/1‑94 (MQ=60)
                                                                                         CCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTACGCTGGCACGAC      <  SRR4302534.3938111/94‑1 (MQ=60)
                                                                                         CCCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTACGCTGGCACGAC      <  SRR4302534.6049437/94‑1 (MQ=60)
                                                                                          CCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTACGCTGGCACGACG     <  SRR4302534.2998833/94‑1 (MQ=60)
                                                                                          CCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTACGCTGGCACGACG     >  SRR4302534.408396/1‑94 (MQ=60)
                                                                                          CCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTACGCTGGCACGACG     <  SRR4302534.2785765/94‑1 (MQ=60)
                                                                                          CCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTACGCTGGCACGACG     <  SRR4302534.2545798/94‑1 (MQ=60)
                                                                                          CCCGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTACGCTGGCACGACG     <  SRR4302534.2195240/94‑1 (MQ=60)
                                                                                            CGGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTACGCTGGCACGACGGC   >  SRR4302534.5087491/1‑94 (MQ=60)
                                                                                             GGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTACGCTGGCACGACGGCG  >  SRR4302534.365462/1‑94 (MQ=60)
                                                                                             GGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTACGCTGGCACGACGGCG  <  SRR4302534.2355605/94‑1 (MQ=60)
                                                                                             |                                                                                             
ACCCGTTACACCAAACGGTATAACCAGGCGAAATCTTCGCAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCAGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTACGCTGGCACGACGGCG  >  NC_000913/4071559‑4071745

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑