Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 884 257.2 12958910 96.9% 12557183 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,088,793 T→C T385T (ACA→ACG)  frvB ← PTS system, fructose‑like enzyme IIBC component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,088,7930TC99.6% 793.7 / ‑4.6 230T385T (ACA→ACG) frvBPTS system, fructose‑like enzyme IIBC component
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (105/124);  total (105/125)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.88e-01

Only 100 of 241 total aligned reads displayed.

CACGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACTGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGACCCGCCGC  >  NC_000913/4088703‑4088882
                                                                                          |                                                                                         
   gCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGc                                                                                      <  2:6243751/93‑1 (MQ=255)
   cccGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGc                                                                                      <  1:6135343/92‑1 (MQ=255)
    ccGTTAATCCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCa                                                                                     <  1:2583075/93‑1 (MQ=255)
     cGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCAc                                                                                    <  1:5234124/93‑1 (MQ=255)
     cGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCAc                                                                                    >  1:2903303/1‑93 (MQ=255)
      ggTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCAcc                                                                                   >  1:4908078/1‑93 (MQ=255)
        tAAGGCGGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGTCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGa                                                                                 <  2:1525133/93‑1 (MQ=255)
           tGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACca                                                                              >  2:5064559/1‑93 (MQ=255)
              cgcccgccAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACaa                                                                           <  2:3141026/93‑1 (MQ=255)
               gcccgccAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAAt                                                                          >  2:4010183/1‑93 (MQ=255)
               gcccgccAGCATATTTGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAAt                                                                          >  2:5429903/1‑93 (MQ=255)
               ccccgccAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAAt                                                                          <  1:1926945/92‑1 (MQ=255)
                cccgccAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATg                                                                         <  1:914755/93‑1 (MQ=255)
                 ccgccAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAACGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGGACATGCCACAATgg                                                                        >  1:6435603/1‑93 (MQ=255)
                  cgccAGCGTATTGGGTGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGc                                                                       <  1:1744992/93‑1 (MQ=255)
                  cgccAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGc                                                                       <  1:5970106/93‑1 (MQ=255)
                  cgccAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGc                                                                       <  1:3269825/93‑1 (MQ=255)
                     cAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACGATGGCact                                                                    >  2:1220307/1‑93 (MQ=255)
                       gCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCactac                                                                  >  2:3977355/1‑93 (MQ=255)
                       gCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCactac                                                                  <  2:372847/93‑1 (MQ=255)
                       gCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCactac                                                                  <  2:4944016/93‑1 (MQ=255)
                       gCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCactac                                                                  <  1:2040785/93‑1 (MQ=255)
                       gCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCaatac                                                                  >  1:3583322/1‑93 (MQ=255)
                        cGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCactact                                                                 <  2:3344324/93‑1 (MQ=255)
                           aTTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGc                                                              <  2:39253/93‑1 (MQ=255)
                           aTTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGc                                                              <  2:5547022/93‑1 (MQ=255)
                                cGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCct                                                         <  2:245031/93‑1 (MQ=255)
                                 ggTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCctc                                                        <  2:2792454/93‑1 (MQ=255)
                                 ggTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCctc                                                        <  2:873386/93‑1 (MQ=255)
                                 ggTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCctc                                                        <  1:5654352/93‑1 (MQ=255)
                                  gTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCctct                                                       <  1:3424134/93‑1 (MQ=255)
                                  gTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCctct                                                       <  2:2653298/93‑1 (MQ=255)
                                   tAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCtt                                                      >  1:2615003/1‑93 (MQ=255)
                                   tAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCtt                                                      <  1:2209641/93‑1 (MQ=255)
                                     atcatcGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCtttt                                                    <  1:1216189/93‑1 (MQ=255)
                                      tcatcGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCttttt                                                   >  2:489716/1‑93 (MQ=255)
                                      gcatcGGGAACGGAGCGGCCCGAGCGTAAGGAATAGCCGGTTAGGTTGCGAACGTGGCACCGACCACAATGGCACTACTGGCAGCCTCttttt                                                   <  1:2604902/92‑1 (MQ=255)
                                        atcGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTcc                                                 >  2:2867789/1‑93 (MQ=255)
                                         tcGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCt                                                <  1:5876443/93‑1 (MQ=255)
                                         tcGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCt                                                <  2:1510476/93‑1 (MQ=255)
                                           ggCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGt                                              >  2:1795270/1‑93 (MQ=255)
                                            gCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTc                                             <  2:262171/93‑1 (MQ=255)
                                              aaCGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCgg                                           <  1:3770984/93‑1 (MQ=255)
                                              aaCGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCgg                                           <  1:348405/93‑1 (MQ=255)
                                              aaCGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCgg                                           <  1:5621187/93‑1 (MQ=255)
                                              aaCGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCgg                                           >  1:1353790/1‑93 (MQ=255)
                                                cGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGt                                         <  1:3308684/93‑1 (MQ=255)
                                                cGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGt                                         >  1:2771468/1‑93 (MQ=255)
                                                cGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGt                                         <  1:1035368/93‑1 (MQ=255)
                                                 ggAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGGACCGACCACAATGGCAATAATGGCAGCCTCCTTTTCCTGTCGGGTa                                        >  2:4440703/1‑93 (MQ=255)
                                                 ggAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTTCTGTCGGGTa                                        >  2:1167848/1‑93 (MQ=255)
                                                  gAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTaa                                       <  2:2512641/93‑1 (MQ=255)
                                                   aGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTaaa                                      >  1:1613661/1‑93 (MQ=255)
                                                     cTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACa                                    <  2:6413778/93‑1 (MQ=255)
                                                      tGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAg                                   >  1:3610789/1‑93 (MQ=255)
                                                       gCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGt                                  <  1:6472734/93‑1 (MQ=255)
                                                       gCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGt                                  <  1:4289268/93‑1 (MQ=255)
                                                        ccAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGtt                                 <  2:2939603/93‑1 (MQ=255)
                                                         cAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGttt                                <  2:6106395/93‑1 (MQ=255)
                                                           gagCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTgg                              <  1:1998547/93‑1 (MQ=255)
                                                           gagCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTgg                              >  1:1251432/1‑93 (MQ=255)
                                                           gagCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTgg                              <  1:4635798/93‑1 (MQ=255)
                                                           gagCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTgg                              >  2:2040217/1‑93 (MQ=255)
                                                             gCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGgc                            >  1:1153862/1‑93 (MQ=255)
                                                             gCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGgc                            <  1:2318951/93‑1 (MQ=255)
                                                             gCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGgc                            >  1:3564113/1‑93 (MQ=255)
                                                               gTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCTGGTAAACAGTTTGGgcgc                          >  1:4674327/1‑93 (MQ=255)
                                                               gTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGgcgc                          >  1:3817175/1‑93 (MQ=255)
                                                               gTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGgcgc                          >  2:2723280/1‑93 (MQ=255)
                                                               gTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGgcgc                          <  2:3837404/93‑1 (MQ=255)
                                                               gTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGgcgc                          >  1:42739/1‑93 (MQ=255)
                                                                 aaGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCaa                        >  2:1595975/1‑93 (MQ=255)
                                                                  aGGAATAGCCGGTTCGGTTGTGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAAt                       <  2:3894758/93‑1 (MQ=255)
                                                                  aGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAAt                       <  2:6062641/93‑1 (MQ=255)
                                                                  aGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAAt                       >  1:5408605/1‑93 (MQ=255)
                                                                  aGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGCAAACAGTTTGGGCGCAAt                       >  1:1114370/1‑93 (MQ=255)
                                                                   ggAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATg                      >  1:3534618/1‑93 (MQ=255)
                                                                   ggAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATg                      <  1:637145/93‑1 (MQ=255)
                                                                   ggAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATg                      >  2:3840555/1‑93 (MQ=255)
                                                                    gAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGa                     >  2:3396473/1‑93 (MQ=255)
                                                                    gAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGa                     >  1:18404/1‑93 (MQ=255)
                                                                     aaTAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGaa                    <  1:740517/93‑1 (MQ=255)
                                                                      aTAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAAc                   >  1:157681/1‑93 (MQ=255)
                                                                       tAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACg                  <  2:5898381/93‑1 (MQ=255)
                                                                           cggttcggttGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGc              >  1:4061615/1‑93 (MQ=255)
                                                                            ggttcggttGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCa             <  1:5106887/93‑1 (MQ=255)
                                                                            ggttcggttGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCa             >  1:6092105/1‑93 (MQ=255)
                                                                            ggttcggttGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCa             >  2:2228753/1‑93 (MQ=255)
                                                                             gttcggttGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCaa            >  2:4199896/1‑93 (MQ=255)
                                                                             gttcggttGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCaa            >  2:1657303/1‑93 (MQ=255)
                                                                              ttcggttGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAg           >  1:3167640/1‑93 (MQ=255)
                                                                               tcggttGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGa          >  1:5027177/1‑93 (MQ=255)
                                                                                cggttGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGAc         >  1:3926651/1‑93 (MQ=255)
                                                                                 ggttGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGAcc        <  2:5619366/93‑1 (MQ=255)
                                                                                 ggttGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGAcc        >  2:4766210/1‑93 (MQ=255)
                                                                                   ttGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGACccg      >  1:5747314/1‑93 (MQ=255)
                                                                                     gCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGACccgcc    <  2:2746951/93‑1 (MQ=255)
                                                                                       gACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGACCcgccgc  <  2:4889116/93‑1 (MQ=255)
                                                                                       gACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGACCcgccgc  >  2:2271494/1‑93 (MQ=255)
                                                                                       gACCGTGCCACCGACCACAATGGCACTACTGTCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGACCcgccgc  <  2:743577/93‑1 (MQ=255)
                                                                                          |                                                                                         
CACGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACTGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGACCCGCCGC  >  NC_000913/4088703‑4088882

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CAGCACGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACTGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGACCCGCCGCGACG  >  NC_000913/4088700‑4088886
                                                                                             |                                                                                             
cagcacccggctaacGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACC                                                                                               <  SRR4302534.1561422/79‑1 (MQ=60)
CAGCACGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACC                                                                                               <  SRR4302534.2988783/94‑1 (MQ=60)
CAGCACGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACC                                                                                               >  SRR4302534.2165712/1‑94 (MQ=60)
CAGCACGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACC                                                                                               >  SRR4302534.2667227/1‑94 (MQ=60)
    caccaggaaatcCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGG                                                                                           <  SRR4302534.5279870/82‑1 (MQ=60)
    ACGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGG                                                                                           >  SRR4302534.1740387/1‑94 (MQ=60)
     CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTGAGGAATAGCCGGTTTGGTTGCCACCGTGGC                                                                                          >  SRR4302534.2670405/1‑94 (MQ=60)
      ccgactatctcccCCCGCCAGCTTATTGGCGGTAATCATCGCCAACGGAGCTGCCAGAGCGTAAGTAATAGCCGGTTCGGTTGCGACCGTGGCA                                                                                         <  SRR4302534.6302235/81‑1 (MQ=60)
      GCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCA                                                                                         <  SRR4302534.2214266/94‑1 (MQ=60)
      CCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCA                                                                                         <  SRR4302534.6302233/94‑1 (MQ=60)
       CCGTTAATCCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCAC                                                                                        <  SRR4302534.2651458/94‑1 (MQ=60)
       CCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCAC                                                                                        >  SRR4302534.2979501/1‑94 (MQ=60)
        CGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACC                                                                                       <  SRR4302534.5375435/94‑1 (MQ=60)
        CGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACC                                                                                       >  SRR4302534.5041119/1‑94 (MQ=60)
             AGGCCCCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCA                                                                                  <  SRR4302534.5798326/94‑1 (MQ=60)
               GCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGAACGTGGCACAGACCACA                                                                                >  SRR4302534.1219143/1‑94 (MQ=60)
               CCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACA                                                                                <  SRR4302534.3057335/94‑1 (MQ=60)
                  CCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATG                                                                             <  SRR4302534.1977324/94‑1 (MQ=60)
                   CCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGG                                                                            <  SRR4302534.938025/94‑1 (MQ=60)
                     CGCCAGCGTATTGGGTGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCA                                                                          <  SRR4302534.1790279/94‑1 (MQ=60)
                     CGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCA                                                                          <  SRR4302534.3356921/94‑1 (MQ=60)
                     CGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCA                                                                          <  SRR4302534.6621620/94‑1 (MQ=60)
                     CGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCA                                                                          <  SRR4302534.6132254/94‑1 (MQ=60)
                      GCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCAC                                                                         >  SRR4302534.2925329/1‑94 (MQ=60)
                       CCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACT                                                                        <  SRR4302534.4795565/94‑1 (MQ=60)
                        CAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACCATGGAACTA                                                                       >  SRR4302534.33311/1‑94 (MQ=60)
                         AGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCATAATGGCACTAC                                                                      >  SRR4302534.6563674/1‑94 (MQ=60)
                         AGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTAC                                                                      <  SRR4302534.5652659/94‑1 (MQ=60)
                         AGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTAC                                                                      >  SRR4302534.2406659/1‑94 (MQ=60)
                         AGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCAATAC                                                                      >  SRR4302534.3679711/1‑94 (MQ=60)
                          GCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACT                                                                     <  SRR4302534.2094138/94‑1 (MQ=60)
                             TATGGGCGGTAATCATCGGCAACGGATCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGC                                                                  <  SRR4302534.6133687/94‑1 (MQ=60)
                              ATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCA                                                                 <  SRR4302534.5299202/94‑1 (MQ=60)
                              ATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCA                                                                 <  SRR4302534.517471/94‑1 (MQ=60)
                               TTGGCGGTATTCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAG                                                                >  SRR4302534.6457256/1‑94 (MQ=60)
                               TTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGCCCGTGGCACCGACCACAATGGCACTACTGGCAG                                                                <  SRR4302534.233579/94‑1 (MQ=60)
                                TGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCCCCGACCACAATGGCACTACTGGCAGC                                                               <  SRR4302534.3630849/94‑1 (MQ=60)
                                TGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGC                                                               >  SRR4302534.1152858/1‑94 (MQ=60)
                                  GCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCT                                                             <  SRR4302534.1226044/94‑1 (MQ=60)
                                  GCCGTAATCATCGGCAACGGAGCTGCCAGAGCTTAATGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCT                                                             <  SRR4302534.1359858/94‑1 (MQ=60)
                                   CGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTC                                                            >  SRR4302534.2126189/1‑94 (MQ=60)
                                    GGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCT                                                           <  SRR4302534.5807422/94‑1 (MQ=60)
                                    GGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCT                                                           >  SRR4302534.3887907/1‑94 (MQ=60)
                                     GTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTT                                                          >  SRR4302534.1637613/1‑94 (MQ=60)
                                     GTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTT                                                          <  SRR4302534.3516135/94‑1 (MQ=60)
                                     GTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTT                                                          >  SRR4302534.2684201/1‑94 (MQ=60)
                                     GTAATCATCGGCAACGAAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGGTGCGACCATGGCATCGATCACAATGTCACTTCAGGCAGGCTCTT                                                          >  SRR4302534.4884327/1‑94 (MQ=46)
                                      TAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTT                                                         <  SRR4302534.3600701/94‑1 (MQ=60)
                                      TAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTT                                                         >  SRR4302534.2674088/1‑94 (MQ=60)
                                      TAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTT                                                         <  SRR4302534.2268143/94‑1 (MQ=60)
                                      TAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTT                                                         >  SRR4302534.1379685/1‑94 (MQ=60)
                                       AATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTT                                                        >  SRR4302534.53961/1‑94 (MQ=60)
                                       AATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTT                                                        <  SRR4302534.1692789/94‑1 (MQ=60)
                                        ATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTT                                                       <  SRR4302534.1248197/94‑1 (MQ=60)
                                         GCATCGGGAACGGAGCGGCCCGAGCGTAAGGAATAGCCGGTTAGGTTGCGAACGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTC                                                      <  SRR4302534.2673827/94‑1 (MQ=60)
                                          CATCGGCACCGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGAACCGACCACAATGGAACTCCTGGCAGCCTCTTTTTCC                                                     >  SRR4302534.5840352/1‑94 (MQ=60)
                                          CATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCC                                                     >  SRR4302534.2532396/1‑94 (MQ=60)
                                          CATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCC                                                     <  SRR4302534.4114542/94‑1 (MQ=60)
                                          CATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCC                                                     >  SRR4302534.4818623/1‑94 (MQ=60)
                                           ATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCT                                                    <  SRR4302534.3679063/94‑1 (MQ=60)
                                           AGCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCATCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCT                                                    <  SRR4302534.3521176/94‑1 (MQ=60)
                                            TCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTG                                                   <  SRR4302534.4133562/94‑1 (MQ=60)
                                            TCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTG                                                   <  SRR4302534.6035985/94‑1 (MQ=60)
                                             CGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGT                                                  >  SRR4302534.1592172/1‑94 (MQ=60)
                                             CGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCAACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGT                                                  <  SRR4302534.3136621/94‑1 (MQ=60)
                                              GGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTC                                                 >  SRR4302534.1953946/1‑94 (MQ=60)
                                               GCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCG                                                <  SRR4302534.4222208/94‑1 (MQ=60)
                                                CAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGG                                               >  SRR4302534.1389472/1‑94 (MQ=60)
                                                 AACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGG                                              <  SRR4302534.357338/94‑1 (MQ=60)
                                                 AACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGG                                              <  SRR4302534.5773296/94‑1 (MQ=60)
                                                 AACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGG                                              <  SRR4302534.2077525/94‑1 (MQ=60)
                                                 AACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGG                                              >  SRR4302534.5373247/1‑94 (MQ=60)
                                                 AACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGG                                              <  SRR4302534.3872248/94‑1 (MQ=60)
                                                  ACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGT                                             >  SRR4302534.2844393/1‑94 (MQ=60)
                                                   CGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTA                                            <  SRR4302534.1062086/94‑1 (MQ=60)
                                                   CGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTA                                            <  SRR4302534.1071429/94‑1 (MQ=60)
                                                   CGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTA                                            <  SRR4302534.3397041/94‑1 (MQ=60)
                                                    GGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAA                                           <  SRR4302534.5660053/94‑1 (MQ=60)
                                                     GAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAA                                          >  SRR4302534.1655775/1‑94 (MQ=60)
                                                     GAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGACACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAA                                          <  SRR4302534.1808951/94‑1 (MQ=60)
                                                      AGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGCCCGTGCCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAAC                                         <  SRR4302534.70667/94‑1 (MQ=60)
                                                      AGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAAC                                         <  SRR4302534.2797067/94‑1 (MQ=60)
                                                        CTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAG                                       >  SRR4302534.3707885/1‑94 (MQ=60)
                                                        CTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAG                                       >  SRR4302534.5931246/1‑94 (MQ=60)
                                                        CTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAG                                       <  SRR4302534.3667814/94‑1 (MQ=60)
                                                         TGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGGCCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGT                                      >  SRR4302534.4403938/1‑94 (MQ=60)
                                                         TGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGT                                      >  SRR4302534.6279488/1‑94 (MQ=60)
                                                          GCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTT                                     <  SRR4302534.6647092/94‑1 (MQ=60)
                                                          GCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTT                                     <  SRR4302534.4405484/94‑1 (MQ=60)
                                                          GCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTT                                     >  SRR4302534.4442091/1‑94 (MQ=60)
                                                            CAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTG                                   >  SRR4302534.3900895/1‑94 (MQ=60)
                                                             AGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGG                                  >  SRR4302534.1284412/1‑94 (MQ=60)
                                                             AGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGG                                  >  SRR4302534.5047579/1‑94 (MQ=60)
                                                             AGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGG                                  >  SRR4302534.339313/1‑94 (MQ=60)
                                                             AGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGG                                  >  SRR4302534.5902015/1‑94 (MQ=60)
                                                             AGAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGG                                  <  SRR4302534.6376014/94‑1 (MQ=60)
                                                              GAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGG                                 <  SRR4302534.2050879/94‑1 (MQ=60)
                                                              GAGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGG                                 <  SRR4302534.4761587/94‑1 (MQ=60)
                                                               AGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGC                                >  SRR4302534.3659988/1‑94 (MQ=60)
                                                               AGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGC                                >  SRR4302534.1184112/1‑94 (MQ=60)
                                                               AGCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGC                                <  SRR4302534.6055061/94‑1 (MQ=60)
                                                                GCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCG                               <  SRR4302534.4651806/94‑1 (MQ=60)
                                                                GCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCG                               <  SRR4302534.2380491/94‑1 (MQ=60)
                                                                GCGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCG                               <  SRR4302534.3697652/94‑1 (MQ=60)
                                                                 CGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCTGGTAAACAGTTTGGGCGC                              >  SRR4302534.4801208/1‑94 (MQ=60)
                                                                 CGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGC                              >  SRR4302534.3380075/1‑94 (MQ=60)
                                                                 CGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGC                              >  SRR4302534.3919714/1‑94 (MQ=60)
                                                                 CGTAAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGC                              >  SRR4302534.43885/1‑94 (MQ=60)
                                                                    AAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAAT                           >  SRR4302534.5554127/1‑94 (MQ=60)
                                                                    AAGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGCAAACAGTTTGGGCGCAAT                           >  SRR4302534.1143462/1‑94 (MQ=60)
                                                                     AGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATG                          <  SRR4302534.2746090/94‑1 (MQ=60)
                                                                     AGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATG                          <  SRR4302534.1279450/94‑1 (MQ=60)
                                                                     AGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATG                          >  SRR4302534.3629697/1‑94 (MQ=60)
                                                                     AGGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATA                          >  SRR4302534.1988280/1‑94 (MQ=60)
                                                                      GGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGA                         <  SRR4302534.652610/94‑1 (MQ=60)
                                                                      GGAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGA                         >  SRR4302534.18894/1‑94 (MQ=60)
                                                                       GAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAA                        <  SRR4302534.1945107/94‑1 (MQ=60)
                                                                       GAATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAA                        >  SRR4302534.392455/1‑94 (MQ=60)
                                                                        AATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAAC                       <  SRR4302534.758866/94‑1 (MQ=60)
                                                                        AATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAAC                       <  SRR4302534.750936/94‑1 (MQ=60)
                                                                        AATAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAAC                       >  SRR4302534.161813/1‑94 (MQ=60)
                                                                        AATAGCCCGTTCGGTTGCCACCGGGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTATGCGCAATGAAG                       >  SRR4302534.3683300/1‑94 (MQ=60)
                                                                          TAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGT                     <  SRR4302534.3470929/94‑1 (MQ=60)
                                                                          TAGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGT                     >  SRR4302534.492891/1‑94 (MQ=60)
                                                                           AGCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTC                    >  SRR4302534.6162046/1‑94 (MQ=60)
                                                                            GCCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCG                   <  SRR4302534.1024945/94‑1 (MQ=60)
                                                                             CCGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGC                  >  SRR4302534.4170570/1‑94 (MQ=60)
                                                                              CGGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCA                 >  SRR4302534.6257772/1‑94 (MQ=60)
                                                                               GGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAA                >  SRR4302534.3770286/1‑94 (MQ=60)
                                                                               GGTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAA                <  SRR4302534.5244701/94‑1 (MQ=60)
                                                                                GTTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAG               >  SRR4302534.3251187/1‑94 (MQ=60)
                                                                                 TTCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGA              >  SRR4302534.5162959/1‑94 (MQ=60)
                                                                                  TCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGAC             <  SRR4302534.931769/94‑1 (MQ=60)
                                                                                  TCGGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGAC             >  SRR4302534.4031841/1‑94 (MQ=60)
                                                                                    GGTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGACCC           >  SRR4302534.3624747/1‑94 (MQ=60)
                                                                                     GTTGCGACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGACCCG          >  SRR4302534.5903150/1‑94 (MQ=60)
                                                                                          GACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGACCCGCCGCG     >  SRR4302534.324592/1‑94 (MQ=60)
                                                                                           ACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGACCCGCCGCGA    >  SRR4302534.6157925/1‑94 (MQ=60)
                                                                                           ACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGACCCGCCGCGA    >  SRR4302534.4830065/1‑94 (MQ=60)
                                                                                           ACCGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGACCCGCCGCGA    >  SRR4302534.940772/1‑94 (MQ=60)
                                                                                           ACCGTGGCACCGACCACAATGGCAATACTGGCAGCCTCTTTTTCCTGACGGGCAAACAGTTTGTGCGCAATGAACGTCGCAAGACCCGCCGCGA    >  SRR4302534.5323736/1‑94 (MQ=60)
                                                                                             CGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGACCCGCCGCGACG  <  SRR4302534.1128726/94‑1 (MQ=60)
                                                                                             |                                                                                             
CAGCACGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAAGGAATAGCCGGTTCGGTTGCGACTGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGACCCGCCGCGACG  >  NC_000913/4088700‑4088886

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑