Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	884	257.2	12958910	96.9%	12557183	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,161,702	C→G	A162G (GCA→GGA)	btuB →	BtuB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,161,702	0	C	G	99.6%	826.1 / NA	234	A162G (GCA→GGA)	btuB	BtuB
Reads supporting (aligned to +/- strand): ref base C (0/1); new base G (113/120); total (113/121)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 246 total aligned reads displayed.

GGCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGCAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATT  >  NC_000913/4161612‑4161791

ggCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTcaggag                                                                                         >  2:3505068/1‑93 (MQ=255)

    gcgcTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCCGGAggggg                                                                                     >  1:2281347/1‑91 (MQ=255)

    gcgcTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTg                                                                                     <  1:4876656/93‑1 (MQ=255)

    gcgcTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGAGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTg                                                                                     >  2:1776584/1‑93 (MQ=255)

      gcTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTggg                                                                                   >  2:1363824/1‑93 (MQ=255)

      gcTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTggg                                                                                   <  1:4496598/93‑1 (MQ=255)

         ccGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGATGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGaa                                                                                >  2:946469/1‑93 (MQ=255)

         ccGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGaa                                                                                >  2:3349176/1‑93 (MQ=255)

          cGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAg                                                                               >  1:1361029/1‑93 (MQ=255)

           gCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGc                                                                              <  1:2131891/93‑1 (MQ=255)

            cTGTTTATGGTTCCGATGCAGTAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCa                                                                             <  2:1475389/93‑1 (MQ=255)

             tGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCaa                                                                            <  2:2827209/93‑1 (MQ=255)

             tGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGGGGGGAAGCaa                                                                            >  1:5580689/1‑93 (MQ=255)

              gTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCaaa                                                                           >  2:4195391/1‑92 (MQ=255)

              gTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAAt                                                                           >  1:461457/1‑93 (MQ=255)

              gTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAAt                                                                           <  1:6072545/93‑1 (MQ=255)

              gTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAAt                                                                           >  1:2856977/1‑93 (MQ=255)

              gTTTATGGTTCCGATGCAATAGGCGGGGTGGAGAATATCATCAAGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGCGGGGGAGCAAt                                                                           >  1:5091778/1‑93 (MQ=255)

               tttATGGTTCCGATGCAATAGGCGGGGTTGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATa                                                                          <  2:6215434/93‑1 (MQ=255)

               tttATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATa                                                                          >  2:5198005/1‑93 (MQ=255)

               tttATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATa                                                                          <  2:1109928/93‑1 (MQ=255)

                ttATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAg                                                                         <  1:1483173/93‑1 (MQ=255)

                ttATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAg                                                                         <  1:4980140/93‑1 (MQ=255)

                 tATGGTTCCGATGCAATAGGCGTGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCATGAGGGTGGGGAAGCAATAGt                                                                        <  1:2985082/93‑1 (MQ=255)

                   tGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTa                                                                      <  1:2486675/93‑1 (MQ=255)

                   tGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTa                                                                      >  1:531998/1‑93 (MQ=255)

                   tGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTa                                                                      >  1:2433488/1‑93 (MQ=255)

                    ggTTCCGATGCAATAGGCGGGTTGGTGAATATCATCACGACGCGCGATGAACCCGGACCGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTAt                                                                     <  1:56196/93‑1 (MQ=255)

                     gTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATc                                                                    <  2:3115929/93‑1 (MQ=255)

                     gTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATc                                                                    >  1:3424613/1‑93 (MQ=255)

                         cGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGaa                                                                <  2:1462727/93‑1 (MQ=255)

                            tGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTa                                                             >  2:5208915/1‑93 (MQ=255)

                            tGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTa                                                             >  2:2985684/1‑93 (MQ=255)

                             gCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTat                                                            <  2:1170239/93‑1 (MQ=255)

                             gCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTat                                                            <  1:1532479/93‑1 (MQ=255)

                              cAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTatg                                                           >  1:1697879/1‑93 (MQ=255)

                               aaTAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTatga                                                          <  2:2100591/93‑1 (MQ=255)

                                aTAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTatgat                                                         >  2:786224/1‑93 (MQ=255)

                                aTAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTatgat                                                         >  2:4497848/1‑93 (MQ=255)

                                   ggCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGtc                                                      >  1:1237996/1‑93 (MQ=255)

                                   ggCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGtc                                                      <  2:3680202/93‑1 (MQ=255)

                                     cGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGtctc                                                    <  1:5963264/93‑1 (MQ=255)

                                     cGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGtctc                                                    <  2:2237121/93‑1 (MQ=255)

                                      ggggTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTctct                                                   <  2:4090514/93‑1 (MQ=255)

                                      ggggTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTctct                                                   <  1:3890360/93‑1 (MQ=255)

                                      ggggTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTctct                                                   <  1:4119432/93‑1 (MQ=255)

                                       gggTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTa                                                  >  2:4610393/1‑93 (MQ=255)

                                       gggTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTa                                                  >  2:2541939/1‑93 (MQ=255)

                                        ggtggtGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTAc                                                 >  2:5318920/1‑93 (MQ=255)

                                        ggtggtGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTAc                                                 <  2:3011253/93‑1 (MQ=255)

                                         gtggtgAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACg                                                >  1:5658892/1‑93 (MQ=255)

                                           ggtgAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACgca                                              >  1:5879541/1‑93 (MQ=255)

                                           ggtgAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACgca                                              >  2:3803887/1‑93 (MQ=255)

                                           ggtgAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACgca                                              <  2:10000/93‑1 (MQ=255)

                                            gtgAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGCGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACgcag                                             >  1:1967284/1‑93 (MQ=255)

                                                atatCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGcaac                                         >  2:5713149/1‑93 (MQ=255)

                                                    catcaCGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACt                                     >  1:3050994/1‑93 (MQ=255)

                                                     atcaCGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTg                                    >  1:812754/1‑93 (MQ=255)

                                                     atcaCGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTg                                    >  2:1623961/1‑93 (MQ=255)

                                                     atcaCGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTg                                    <  1:4464333/93‑1 (MQ=255)

                                                     atcaCGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTg                                    <  1:5554143/93‑1 (MQ=255)

                                                      tcaCGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTgg                                   >  2:2762929/1‑93 (MQ=255)

                                                          gacgCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGa                               <  2:4633280/93‑1 (MQ=255)

                                                          gacgCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGa                               >  2:2400620/1‑93 (MQ=255)

                                                           acgCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGAt                              <  1:6330689/93‑1 (MQ=255)

                                                             gcgcgATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATaa                            <  2:4413444/93‑1 (MQ=255)

                                                               gcgATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGa                          >  2:3775619/1‑93 (MQ=255)

                                                                cgATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGac                         >  1:2953554/1‑93 (MQ=255)

                                                                cgATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGac                         >  2:5464998/1‑93 (MQ=255)

                                                                cgATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGac                         <  2:5834694/93‑1 (MQ=255)

                                                                 gATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGaca                        >  2:6038926/1‑93 (MQ=255)

                                                                 gATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGaca                        <  1:5176086/93‑1 (MQ=255)

                                                                  aTGAACCCGGAGCGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGacac                       <  1:6212672/93‑1 (MQ=255)

                                                                  aTGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGacac                       >  2:6448415/1‑93 (MQ=255)

                                                                   tGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACg                      >  2:4891622/1‑93 (MQ=255)

                                                                   tGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACg                      >  2:3857882/1‑93 (MQ=255)

                                                                    gaccccGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACgg                     <  2:1846548/90‑1 (MQ=255)

                                                                    gAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACgg                     >  2:3913428/1‑93 (MQ=255)

                                                                    gAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACgg                     >  1:1030409/1‑93 (MQ=255)

                                                                    gAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCGACGCAGCAACAACTGGGGGATAAGACACgg                     <  1:3173465/93‑1 (MQ=255)

                                                                     aaCCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACggg                    >  1:2171073/1‑93 (MQ=255)

                                                                      aCCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGt                   >  1:6281882/1‑93 (MQ=255)

                                                                       cccGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTa                  <  1:854829/93‑1 (MQ=255)

                                                                       cccGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTa                  <  1:5473864/93‑1 (MQ=255)

                                                                         cggaacggaaATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAAc                >  1:490952/1‑93 (MQ=255)

                                                                         cggaacggaaATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAAc                <  2:222858/93‑1 (MQ=255)

                                                                           gaacggaaATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGc              >  2:125751/1‑93 (MQ=255)

                                                                           gaacggaaATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGc              >  1:431092/1‑93 (MQ=255)

                                                                           gaacggaaATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGc              <  1:3603912/93‑1 (MQ=255)

                                                                            aacggaaATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCt             <  1:5803034/93‑1 (MQ=255)

                                                                             acggaaATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTg            >  1:4886172/1‑93 (MQ=255)

                                                                             acggaaATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTg            <  2:4161139/93‑1 (MQ=255)

                                                                                gaaATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTg         <  2:4828217/93‑1 (MQ=255)

                                                                                gaaATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTg         <  1:4836881/93‑1 (MQ=255)

                                                                                   aTTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGc      >  2:590890/1‑93 (MQ=255)

                                                                                   aTTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGc      >  1:5050463/1‑93 (MQ=255)

                                                                                    tttCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCg     <  2:2632105/93‑1 (MQ=255)

                                                                                      tCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGAt   <  1:1598424/93‑1 (MQ=255)

                                                                                       caggagGGTGGGGGAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGAtt  >  1:3858430/1‑93 (MQ=255)

                                                                                       caggagGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGAtt  <  2:5537003/93‑1 (MQ=255)

GGCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGCAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATT  >  NC_000913/4161612‑4161791

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GTGGGCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGCAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTATG  >  NC_000913/4161609‑4161794

GTGGGCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGG                                                                                              >  SRR4302534.3133197/1‑94 (MQ=60)

 TGGGCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGA                                                                                             >  SRR4302534.224819/1‑94 (MQ=60)

  GGGCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAG                                                                                            <  SRR4302534.5136601/94‑1 (MQ=60)

   GGCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGG                                                                                           >  SRR4302534.5816010/1‑94 (MQ=60)

     CCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGT                                                                                         <  SRR4302534.5721422/94‑1 (MQ=60)

      CGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTG                                                                                        <  SRR4302534.5682635/94‑1 (MQ=60)

       GCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGG                                                                                       <  SRR4302534.5008926/94‑1 (MQ=60)

        CGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGG                                                                                      <  SRR4302534.4499551/94‑1 (MQ=60)

         GCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGG                                                                                     <  SRR4302534.4618292/94‑1 (MQ=60)

         GCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGG                                                                                     <  SRR4302534.1552224/94‑1 (MQ=60)

          CTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGA                                                                                    >  SRR4302534.5224255/1‑94 (MQ=60)

          CTCCGCTGTTTATGGTCCCGAGGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGA                                                                                    <  SRR4302534.5783913/94‑1 (MQ=60)

          CTCCGCTGGTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGA                                                                                    >  SRR4302534.6531553/1‑94 (MQ=60)

           TCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAA                                                                                   >  SRR4302534.2945727/1‑94 (MQ=60)

            CCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAG                                                                                  <  SRR4302534.892348/94‑1 (MQ=60)

            CCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAG                                                                                  <  SRR4302534.4656931/94‑1 (MQ=60)

            CCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAG                                                                                  >  SRR4302534.1396903/1‑94 (MQ=60)

              GCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCA                                                                                <  SRR4302534.2188058/94‑1 (MQ=60)

              GCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCA                                                                                >  SRR4302534.865464/1‑94 (MQ=60)

               CTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGGGGGGAAGCAA                                                                               >  SRR4302534.5731464/1‑94 (MQ=60)

                TGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAAT                                                                              >  SRR4302534.473113/1‑94 (MQ=60)

                TGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAAT                                                                              >  SRR4302534.4282706/1‑94 (MQ=60)

                TGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAAT                                                                              >  SRR4302534.5226626/1‑94 (MQ=60)

                TGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAAT                                                                              >  SRR4302534.2932021/1‑94 (MQ=60)

                TGTTTATGGTTCCGATGCAATAGGCGGGGTGGAGAATATCATCAAGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGCGGGGGAGCAAT                                                                              >  SRR4302534.5229212/1‑94 (MQ=60)

                 TTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATA                                                                             >  SRR4302534.3974862/1‑94 (MQ=60)

                 GTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATA                                                                             <  SRR4302534.6237656/94‑1 (MQ=60)

                   TTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGT                                                                           <  SRR4302534.5114932/94‑1 (MQ=60)

                   TTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGT                                                                           <  SRR4302534.1522191/94‑1 (MQ=60)

                   TTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGT                                                                           <  SRR4302534.3560469/94‑1 (MQ=60)

                    TATGGTTCCGATGCAATAGGCGTGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCATGAGGGTGGGGAAGCAATAGTT                                                                          <  SRR4302534.3063157/94‑1 (MQ=60)

                    TATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTT                                                                          >  SRR4302534.148433/1‑94 (MQ=60)

                    TATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTT                                                                          <  SRR4302534.1623556/94‑1 (MQ=60)

                     ATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTA                                                                         >  SRR4302534.2498173/1‑94 (MQ=60)

                     ATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTA                                                                         >  SRR4302534.545204/1‑94 (MQ=60)

                      TGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTAT                                                                        <  SRR4302534.2552737/94‑1 (MQ=60)

                       GGTTCCGATGCAATAGGCGGGTTGGTGAATATCATCACGACGCGCGATGAACCCGGACCGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATC                                                                       <  SRR4302534.57715/94‑1 (MQ=60)

                       GGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATC                                                                       <  SRR4302534.2198265/94‑1 (MQ=60)

                       GGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATC                                                                       >  SRR4302534.3516622/1‑94 (MQ=60)

                        GTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCA                                                                      <  SRR4302534.5020359/94‑1 (MQ=60)

                        GTTCCGATGCAATAGGCGGGGTGGAGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCA                                                                      >  SRR4302534.3701188/1‑94 (MQ=60)

                         TTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAG                                                                     >  SRR4302534.5994962/1‑94 (MQ=60)

                             GATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACT                                                                 >  SRR4302534.3998891/1‑94 (MQ=60)

                               TGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTAT                                                               <  SRR4302534.5559490/94‑1 (MQ=60)

                                GCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATG                                                              <  SRR4302534.1572681/94‑1 (MQ=60)

                                GCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATG                                                              >  SRR4302534.1741998/1‑94 (MQ=60)

                                 cttccgatctatagattAATATCATCACGACGCGCGACGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGA                                                             >  SRR4302534.3118864/18‑94 (MQ=60)

                                 CAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGA                                                             >  SRR4302534.289896/1‑94 (MQ=60)

                                 CAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGA                                                             >  SRR4302534.3774892/1‑94 (MQ=60)

                                 CAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACACGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGA                                                             >  SRR4302534.4800272/1‑94 (MQ=60)

                                  AATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGAT                                                            <  SRR4302534.4607493/94‑1 (MQ=60)

                                  AATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGAT                                                            >  SRR4302534.789241/1‑94 (MQ=60)

                                    TAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGT                                                          <  SRR4302534.3036144/94‑1 (MQ=60)

                                    TAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGT                                                          <  SRR4302534.5756057/94‑1 (MQ=60)

                                     AGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTC                                                         <  SRR4302534.5989296/94‑1 (MQ=60)

                                     AGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTC                                                         <  SRR4302534.4976589/94‑1 (MQ=60)

                                     AGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTC                                                         >  SRR4302534.1270628/1‑94 (MQ=60)

                                      GGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCT                                                        <  SRR4302534.5050296/94‑1 (MQ=60)

                                        CGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCT                                                      <  SRR4302534.6125181/94‑1 (MQ=60)

                                        CGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCT                                                      <  SRR4302534.1344872/94‑1 (MQ=60)

                                        CGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCT                                                      >  SRR4302534.1621801/1‑94 (MQ=60)

                                         GGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTA                                                     >  SRR4302534.5017870/1‑94 (MQ=60)

                                         GGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTA                                                     <  SRR4302534.2465058/94‑1 (MQ=60)

                                         GGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTA                                                     <  SRR4302534.4230285/94‑1 (MQ=60)

                                         GGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTA                                                     <  SRR4302534.3994795/94‑1 (MQ=60)

                                           GGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACG                                                   >  SRR4302534.5812091/1‑94 (MQ=60)

                                             TGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCA                                                 >  SRR4302534.6039176/1‑94 (MQ=60)

                                              GGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAG                                                >  SRR4302534.5173376/1‑94 (MQ=60)

                                              GGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGCGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAG                                                >  SRR4302534.2018780/1‑94 (MQ=60)

                                                TGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCA                                              <  SRR4302534.4931712/94‑1 (MQ=60)

                                                TGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCA                                              >  SRR4302534.6213170/1‑94 (MQ=60)

                                                 GAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAA                                             <  SRR4302534.1695556/94‑1 (MQ=60)

                                                  AATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAAC                                            >  SRR4302534.3024551/1‑94 (MQ=60)

                                                   ATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACA                                           >  SRR4302534.2180768/1‑94 (MQ=60)

                                                   ATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACA                                           >  SRR4302534.612527/1‑94 (MQ=60)

                                                    TATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAA                                          >  SRR4302534.1432167/1‑94 (MQ=60)

                                                     ATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAAC                                         <  SRR4302534.979156/94‑1 (MQ=60)

                                                      TCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACT                                        >  SRR4302534.3131041/1‑94 (MQ=60)

                                                       CATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTG                                       >  SRR4302534.564676/1‑94 (MQ=60)

                                                       CATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTG                                       >  SRR4302534.833176/1‑94 (MQ=60)

                                                       CATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTG                                       >  SRR4302534.6070272/1‑94 (MQ=60)

                                                        ATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGG                                      <  SRR4302534.5704086/94‑1 (MQ=60)

                                                        ATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGG                                      <  SRR4302534.3316134/94‑1 (MQ=60)

                                                        ATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGG                                      <  SRR4302534.4585051/94‑1 (MQ=60)

                                                          CACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGG                                    <  SRR4302534.1091806/94‑1 (MQ=60)

                                                            CGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGA                                  <  SRR4302534.416424/94‑1 (MQ=60)

                                                              GCGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATA                                <  SRR4302534.3106343/94‑1 (MQ=60)

                                                              ACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATA                                <  SRR4302534.6501731/94‑1 (MQ=60)

                                                                  GCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGAC                            >  SRR4302534.3030983/1‑94 (MQ=60)

                                                                   CGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACA                           >  SRR4302534.5436334/1‑94 (MQ=60)

                                                                    GATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACAC                          >  SRR4302534.4531479/1‑94 (MQ=60)

                                                                    GATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACAC                          <  SRR4302534.5315561/94‑1 (MQ=60)

                                                                     ATGAACCCGGAGCGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACG                         <  SRR4302534.6381497/94‑1 (MQ=60)

                                                                     ATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACG                         >  SRR4302534.5771989/1‑94 (MQ=60)

                                                                      TGAACCCGGAACGGAAATTTCAGGAGGGTGGTGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGG                        <  SRR4302534.3325982/94‑1 (MQ=60)

                                                                      TGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGG                        >  SRR4302534.2364083/1‑94 (MQ=60)

                                                                      TGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGG                        >  SRR4302534.1056957/1‑94 (MQ=60)

                                                                       GAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGG                       >  SRR4302534.2228425/1‑94 (MQ=60)

                                                                       GAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCGACGCAGCAACAACTGGGGGATAAGACACGGG                       <  SRR4302534.3257227/94‑1 (MQ=60)

                                                                        AACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGT                      >  SRR4302534.5018310/1‑94 (MQ=60)

                                                                        AACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGT                      >  SRR4302534.6451881/1‑94 (MQ=60)

                                                                         ACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTATGCAGCAACAACTGGGGGATAAGACACGGGTA                     <  SRR4302534.255051/94‑1 (MQ=60)

                                                                          CCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAA                    <  SRR4302534.5621452/94‑1 (MQ=60)

                                                                          CCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAA                    <  SRR4302534.876400/94‑1 (MQ=60)

                                                                           CCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAAC                   >  SRR4302534.503209/1‑94 (MQ=60)

                                                                           CCGGAACGGAAATGTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAAC                   >  SRR4302534.5290956/1‑94 (MQ=60)

                                                                             GGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGC                 >  SRR4302534.442092/1‑94 (MQ=60)

                                                                              GAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCT                <  SRR4302534.3700821/94‑1 (MQ=60)

                                                                              GAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCT                <  SRR4302534.5450043/94‑1 (MQ=60)

                                                                               AACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTG               >  SRR4302534.5018684/1‑94 (MQ=60)

                                                                               AACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTG               <  SRR4302534.5960517/94‑1 (MQ=60)

                                                                               AACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTG               <  SRR4302534.644934/94‑1 (MQ=60)

                                                                                 CGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTT             <  SRR4302534.2270701/94‑1 (MQ=60)

                                                                                 CGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTT             <  SRR4302534.1881716/94‑1 (MQ=60)

                                                                                 CGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTT             <  SRR4302534.6382737/94‑1 (MQ=60)

                                                                                  GGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTG            >  SRR4302534.2208133/1‑94 (MQ=60)

                                                                                   GAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGG           <  SRR4302534.4968098/94‑1 (MQ=60)

                                                                                    AAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGG          <  SRR4302534.6355130/94‑1 (MQ=60)

                                                                                     AATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGC         >  SRR4302534.5186838/1‑94 (MQ=60)

                                                                                      ATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCG        <  SRR4302534.509502/94‑1 (MQ=60)

                                                                                         TCAGGAGGGTGGGGGAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATT     >  SRR4302534.3962021/1‑94 (MQ=60)

                                                                                         TCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATT     >  SRR4302534.6402471/1‑94 (MQ=60)

                                                                                         TCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATT     <  SRR4302534.3174526/94‑1 (MQ=60)

                                                                                         TCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATT     <  SRR4302534.1640186/94‑1 (MQ=60)

                                                                                         TCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATT     <  SRR4302534.3112116/94‑1 (MQ=60)

                                                                                          CAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTA    <  SRR4302534.1959254/94‑1 (MQ=60)

                                                                                          CAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTA    >  SRR4302534.3870660/1‑94 (MQ=60)

                                                                                          CAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTA    >  SRR4302534.5809886/1‑94 (MQ=60)

                                                                                           AGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTAT   <  SRR4302534.3968613/94‑1 (MQ=60)

                                                                                           AGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTAT   <  SRR4302534.5022873/94‑1 (MQ=60)

                                                                                            GGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTATG  >  SRR4302534.1547475/1‑94 (MQ=60)

                                                                                            GGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTATG  >  SRR4302534.951826/1‑94 (MQ=60)

GTGGGCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGCAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTATG  >  NC_000913/4161609‑4161794

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`