Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 884 257.2 12958910 96.9% 12557183 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,170,208 T→C G191G (GGT→GGC)  murB → UDP‑N‑acetylenolpyruvoylglucosamine reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,170,2080TC99.6% 888.4 / ‑4.6 258G191G (GGT→GGC) murBUDP‑N‑acetylenolpyruvoylglucosamine reductase
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (133/124);  total (134/124)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.53e-01

Only 100 of 258 total aligned reads displayed.

TATTTTTAAACATGAATACCAGGACCGCTTCGCTATTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGTGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCAAGTATTTAATGCGGTGTGTCATATGCGCACCACCAAACTCCCTGATCC  >  NC_000913/4170118‑4170297
                                                                                          |                                                                                         
tATTTTTAAACATGAATACCAGGACCGCTTCGCTATTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGa                                                                                         <  2:5042915/93‑1 (MQ=255)
 aTTTTTAAACATGAATACCAGGACCGCTTCGCTATTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGAc                                                                                        <  1:3442538/93‑1 (MQ=255)
 aTTTTTAAACATGAATACCAGGACCGCTTCGCTATTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGAc                                                                                        <  2:4661517/93‑1 (MQ=255)
 aTTTTTAAACATGAATACCAGGACCGCTTCGCTATTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGAc                                                                                        <  2:32572/93‑1 (MQ=255)
  tttttAAACATGAATACCAGGACCGCTTCGCTATTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACt                                                                                       <  2:2260165/93‑1 (MQ=255)
  tttttAAACATGAATACCAGGACCGCTTCGCTATTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACt                                                                                       <  1:6054001/93‑1 (MQ=255)
   ttttAAACATGAATACCAGGACCGCTTCGCTATTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACtt                                                                                      <  2:1352619/93‑1 (MQ=255)
   ttttAAACATGAATACCAGGACCGCTTCGCTATTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACtt                                                                                      <  2:1775124/93‑1 (MQ=255)
       aaaCATGAATACCAGGACCGCTTCGCTATTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACt                                                                                  >  1:5622597/1‑93 (MQ=255)
       aaaCATGAATACCAGGACCGCTTCGCTATTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACt                                                                                  >  1:2623694/1‑93 (MQ=255)
        atcATGAATACAAGGACCGCTTCGCTATTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTc                                                                                 <  1:4470827/91‑1 (MQ=255)
         aCATGAATACCAGGACCGCTTCGCTATTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCg                                                                                >  1:1859191/1‑93 (MQ=255)
         aCATGAATACCAGGACCGCTTCGCTATTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCg                                                                                <  1:6274697/93‑1 (MQ=255)
          cATGAATACCAGGACCGCTTCGCTATTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGt                                                                               >  2:5056292/1‑93 (MQ=255)
           aTGAATACCAGCACCGCTTCGCTATTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTc                                                                              <  1:472983/93‑1 (MQ=255)
            tGAATACCAGGACCGCTTCGCTATTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCt                                                                             >  2:2902519/1‑93 (MQ=255)
             gAATACCAGGACCGCTTCGCTATTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTg                                                                            <  1:647760/93‑1 (MQ=255)
              aaTACCAGGACCGCTTCGCTATTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGAACTAACGTATGGCGACTTAACTCGTCTgg                                                                           <  1:2968125/93‑1 (MQ=255)
                tACCAGGACCGCTTCGCTATTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGAt                                                                         <  2:1104356/93‑1 (MQ=255)
                 aCCAGGACCGCTTCGCTATTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATc                                                                        >  1:3184876/1‑93 (MQ=255)
                  ccAGGACCGCTTCGCTATTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATcc                                                                       >  2:5290583/1‑93 (MQ=255)
                  ccAGGACCGCTTCGCTATTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATcc                                                                       <  1:758180/93‑1 (MQ=255)
                   ctggACCGCTTCGCTATTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCt                                                                      <  1:1608912/91‑1 (MQ=255)
                   cAGGACCGCTTCGCTATTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCt                                                                      <  1:2051202/93‑1 (MQ=255)
                    aGGACCGCTTCGCTATTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCtc                                                                     >  1:4226257/1‑92 (MQ=255)
                     ggACCGCTTCGCTATTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTac                                                                    >  2:2074831/1‑93 (MQ=255)
                     ggACCGCTTCGCTATTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTac                                                                    >  1:3034399/1‑93 (MQ=255)
                      gACCGCTTCGCTATTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTaca                                                                   >  2:2659094/1‑93 (MQ=255)
                      gACCGCTTCGCTATTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTaca                                                                   >  1:2901274/1‑93 (MQ=255)
                       aCCGCTTCGCTATTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTacaa                                                                  >  1:5352371/1‑93 (MQ=255)
                       aCCGCTTCGCTATTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTacaa                                                                  >  1:4298295/1‑93 (MQ=255)
                       aCCGCTTCGCTATTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAAAGTATGGCGACTTAACTCGTCTGGATCCTacaa                                                                  <  2:2093585/93‑1 (MQ=255)
                         cGCTTCGCTATTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTacaaca                                                                <  1:4705530/93‑1 (MQ=255)
                          gCTTCGCTATTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAg                                                               >  1:936146/1‑93 (MQ=255)
                           cTTCGCTATTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAga                                                              >  1:4815335/1‑92 (MQ=255)
                             tCGCTATTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTaa                                                            <  2:586668/93‑1 (MQ=255)
                               gCTATTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTATCGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACg                                                          >  1:5786677/1‑93 (MQ=255)
                               gCTATTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACg                                                          >  1:3564163/1‑93 (MQ=255)
                                cTATTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGc                                                         <  2:5578416/93‑1 (MQ=255)
                                 tATTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGcc                                                        >  2:1244545/1‑93 (MQ=255)
                                 tATTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGcc                                                        >  2:3158949/1‑93 (MQ=255)
                                 tATTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGcc                                                        <  2:2810936/93‑1 (MQ=255)
                                  aTTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCca                                                       >  1:3110016/1‑93 (MQ=255)
                                   ttGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCcac                                                      >  1:5528846/1‑93 (MQ=255)
                                   ttGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCcac                                                      >  1:1549400/1‑93 (MQ=255)
                                   ttGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCcac                                                      >  1:5442144/1‑93 (MQ=255)
                                   ttGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCcac                                                      <  1:5101399/93‑1 (MQ=255)
                                   ttGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCcac                                                      >  1:3852269/1‑93 (MQ=255)
                                    tGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCcaca                                                     >  1:3723508/1‑93 (MQ=255)
                                     ttAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAg                                                    <  2:4395490/92‑1 (MQ=255)
                                      tAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGc                                                   >  2:6106690/1‑93 (MQ=255)
                                        gCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCaa                                                 <  1:832142/93‑1 (MQ=255)
                                           gTAGGTCTGCGTCTGCCAAAAGAGTGGCACCCTGTGCTAACGTATGGCGACTTATCTCGTCTGGTTCCTACACCAGTAACGCCACAGCAAGTa                                              <  1:4128946/93‑1 (MQ=255)
                                           gTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCAAGTa                                              >  2:2026424/1‑93 (MQ=255)
                                            tAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCAAGTAt                                             <  1:234356/93‑1 (MQ=255)
                                             aGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCAAGTAtt                                            >  2:3341353/1‑93 (MQ=255)
                                             aGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCAAGTAtt                                            >  2:5379781/1‑93 (MQ=255)
                                              ggTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCAAGTAttt                                           >  2:949333/1‑93 (MQ=255)
                                              ggTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCAAGTAttt                                           >  1:6163096/1‑93 (MQ=255)
                                               gtctgcgtctgcCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCAAGTATTTa                                          <  2:3545075/93‑1 (MQ=255)
                                                tctgcgtctgcCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCAAGTATTTaa                                         <  2:5321568/93‑1 (MQ=255)
                                                tctgcgtctgcCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCAAGTATTTaa                                         >  2:4883779/1‑93 (MQ=255)
                                                   gcgtctgcCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCAAGTATTTAATGc                                      >  1:795521/1‑93 (MQ=255)
                                                    cgtctgcCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCAAGTATTTAATGCg                                     <  2:4053953/93‑1 (MQ=255)
                                                    cgtctgcCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCAAGTATTTAATGCg                                     >  2:4002496/1‑93 (MQ=255)
                                                     gtctgcCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCAAGTATTTAATGCgg                                    >  2:801309/1‑93 (MQ=255)
                                                      tctgcCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCAAGTATTTAATGCGgt                                   >  2:3047268/1‑93 (MQ=255)
                                                      tctgcCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCAAGTATTTAATGCGgt                                   <  2:4115797/93‑1 (MQ=255)
                                                      tctgcCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCAAGTATTTAATGCGgt                                   <  1:5680250/93‑1 (MQ=255)
                                                          ccAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCAAGTATTTAATGCGgtgtgt                               >  2:3416935/1‑93 (MQ=255)
                                                          ccAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCAAGTATTTAATGCGgtgtgt                               >  2:2335775/1‑93 (MQ=255)
                                                          ccAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCAAGTATTTAATGCGgtgtgt                               >  2:6218471/1‑93 (MQ=255)
                                                            aaaaGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCAAGTATTTAATGCGGTGTGTCa                             <  1:3362338/93‑1 (MQ=255)
                                                            aaaaGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCAAGTATTTAATGCGGTGTGTCa                             >  2:725203/1‑93 (MQ=255)
                                                            aaaaGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCAAGTATTTAATGCGGTGTGTCa                             <  2:1919381/93‑1 (MQ=255)
                                                             aaaGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCAAGTATTTAATGCGGTGTGTCat                            <  2:2972213/93‑1 (MQ=255)
                                                             aaaGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCAAGTATTTAATGCGGTGTGTCat                            <  1:435066/93‑1 (MQ=255)
                                                             aaaGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCAAGTATTTAATGCGGTGTGTCat                            <  2:4260905/93‑1 (MQ=255)
                                                              aaGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCAAGTATTTAATGCGGTTTGTCata                           <  2:6385098/93‑1 (MQ=255)
                                                              aaGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCAAGTATTTAATGCGGTGTGTCata                           >  1:1240140/1‑93 (MQ=255)
                                                              aaGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCAAGTATTTAATGCGGTGTGTCata                           <  2:2493371/93‑1 (MQ=255)
                                                               agagTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCAAGTATTTAATGCGGTGTGTCatat                          >  2:836482/1‑93 (MQ=255)
                                                               agagTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCAAGTATTTAATGCGGTGTGTCatat                          <  1:3516233/93‑1 (MQ=255)
                                                                gagTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCAAGTATTTAATGCGGTGTGTCATATg                         >  2:683990/1‑93 (MQ=255)
                                                                 agTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCAAGTATTTAATGCGGTGTGTCATATgc                        <  2:5989778/93‑1 (MQ=255)
                                                                    ggCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCAAGTATTTAATGCGGTGTGTCATATGCGca                     >  2:2850595/1‑93 (MQ=255)
                                                                       aaCCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCAAGTATTTAATGCGGTGTGTCATATGCGcacca                  <  2:5000812/93‑1 (MQ=255)
                                                                       aaCCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCAAGTATTTAATGCGGTGTGTCATATGCGcacca                  >  1:5984772/1‑93 (MQ=255)
                                                                          cTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCAAGTATTTAATGCGGTGTGTCATATGCGCAccacca               <  1:5585568/93‑1 (MQ=255)
                                                                          cTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCAAGTATTTAATGCGGTGTGTCATATGCGCAccacca               <  2:1496445/93‑1 (MQ=255)
                                                                             tACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCAAGTATTTAATGCGGTGTGTCATATGCGCACCACCAAAc            <  1:3415473/93‑1 (MQ=255)
                                                                              aCTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCAAGTATTTAATGCGGTGTGTCATATGCGCACCACCAAACt           <  2:1645012/93‑1 (MQ=255)
                                                                               cTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCAAGTATTTAATGCGGTGTGTCATATGCGCACCACCAAACTc          >  1:1098862/1‑93 (MQ=255)
                                                                               cTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCAAGTATTTAATGCGGTGTGTCATATGCGCACCACCAAACTc          >  2:153399/1‑93 (MQ=255)
                                                                                tAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCAAGTATTTAATGCGGTGTGTCATATGCGCACCACCAAACTcc         <  2:5736253/93‑1 (MQ=255)
                                                                                tAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCAAGTATTTAATGCGGTGTGTCATATGCGCACCACCAAACTcc         >  1:2252181/1‑93 (MQ=255)
                                                                                 aaCGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCAAGTATTTAATGCGGTGTGTCATATGCGCACCACCAAACTccc        <  1:5944042/93‑1 (MQ=255)
                                                                                  aCGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCAAGTATTTAATGCGGTGTGTCATATGCGCACCACCAAACTCCCt       >  1:5010235/1‑93 (MQ=255)
                                                                                     tATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCAAGTATTTAATGCGGTGTGTCATATGCGCACCACCAAACTCCCTGAt    >  1:2177222/1‑93 (MQ=255)
                                                                                      aTGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCAAGTATTTAATGCGGTGTGTCATATGCGCCCCACCAAACTCCCTGATc   <  1:2509524/93‑1 (MQ=255)
                                                                                          |                                                                                         
TATTTTTAAACATGAATACCAGGACCGCTTCGCTATTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGTGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCAAGTATTTAATGCGGTGTGTCATATGCGCACCACCAAACTCCCTGATCC  >  NC_000913/4170118‑4170297

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATTTTTAAACATGAATACCAGGACCGCTTCGCTATTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGTGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCAAGTATTTAATGCGGTGTGTCATATGCGCACCACCAAACTCCCTGATCCAAAA  >  NC_000913/4170119‑4170301
                                                                                         |                                                                                             
ATTTTTAAACATGAATACCAGGACCGCTTCGCTATTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACT                                                                                           >  SRR4302534.542057/1‑94 (MQ=60)
ATTTTTAAACATGAATACCAGGACCGCTTCGCTATTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACT                                                                                           <  SRR4302534.3535064/94‑1 (MQ=60)
 TTTTTAAACATGAATACCAGGACCGCTTCGCTATTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTT                                                                                          <  SRR4302534.6218630/94‑1 (MQ=60)
   TTTAAACATGAATACCAGGACCGCTTCGCTATTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAA                                                                                        >  SRR4302534.3136089/1‑94 (MQ=60)
     TAAACATGAATACCAGGACCGCTTCGCTATTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACT                                                                                      >  SRR4302534.5805720/1‑94 (MQ=60)
     TAAACATGAATACCAGGACCGCTTCGCTATTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACT                                                                                      >  SRR4302534.2693102/1‑94 (MQ=60)
     TAAACATGAATACCAGGACCGCTTCGCTATTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACT                                                                                      >  SRR4302534.5774744/1‑94 (MQ=60)
     TAAACATGAATACCAGGACCGCTTCGCTATTGTAGCCGTAGGTCTGCGTCTGACAAAAGAGTGGCAACTAGTACTACCGTATGGCGACTTAACT                                                                                      <  SRR4302534.2096193/94‑1 (MQ=60)
      AAACATGAATACCAGGACCGCTTCGCTATTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTC                                                                                     <  SRR4302534.4455755/94‑1 (MQ=60)
      AAACATGAATACCAGGACCGCTTCGCTATTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTC                                                                                     <  SRR4302534.6154151/94‑1 (MQ=60)
       ATCATGAATACAAGGACCGCTTCGCTATTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCG                                                                                    <  SRR4302534.4591739/94‑1 (MQ=60)
       AACATGAATACCAGGACCGCTTCGCTATTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCG                                                                                    >  SRR4302534.1907591/1‑94 (MQ=60)
        ACATGAATACCAGGACCGCTTCGCTATTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGT                                                                                   <  SRR4302534.6444557/94‑1 (MQ=60)
         CATGAATACCAGGACCGCTTCGCTATTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTC                                                                                  >  SRR4302534.3097834/1‑94 (MQ=60)
          ATGAATACCAGGACCGCTTCGCTATTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCT                                                                                 <  SRR4302534.48455/94‑1 (MQ=60)
          ATGAATACCAGCACCGCTTCGCTATTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCT                                                                                 <  SRR4302534.484889/94‑1 (MQ=60)
           TGAATACCAGGACCGCTTCGCTATTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTG                                                                                <  SRR4302534.2267268/94‑1 (MQ=60)
            GAATACCAGGACCGCTTCGCTATTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGG                                                                               <  SRR4302534.663467/94‑1 (MQ=60)
            GAATACCAGGACCGCTTCGCTATTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGG                                                                               <  SRR4302534.4439900/94‑1 (MQ=60)
             AATACCAGGACCGCTTCGCTATTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGA                                                                              <  SRR4302534.3387567/94‑1 (MQ=60)
             AATACCAGGACCGCTTCGCTATTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGAACTAACGTATGGCGACTTAACTCGTCTGGA                                                                              <  SRR4302534.3045853/94‑1 (MQ=60)
               TACCAGGACCGCTTCGCTATTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATC                                                                            >  SRR4302534.3269098/1‑94 (MQ=60)
               TACCAGGACCGCTTCGCTATTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATC                                                                            <  SRR4302534.3655037/94‑1 (MQ=60)
               AACCAGGACCCATTCGCTGTTGTACCCGTAGGGCTGCGTCTGCCAAAACAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATC                                                                            <  SRR4302534.912280/94‑1 (MQ=60)
                ACCAGGACCGCTTCGCTATTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCC                                                                           <  SRR4302534.439481/94‑1 (MQ=60)
                 CCAGGACCGCTTCGCTATTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTCACTCGTCTGGATCCT                                                                          >  SRR4302534.3511153/1‑94 (MQ=60)
                 CCAGGACCGCTTCGCTATTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCT                                                                          <  SRR4302534.776985/94‑1 (MQ=60)
                  CTGGACCGCTTCGCTATTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTA                                                                         <  SRR4302534.1650914/94‑1 (MQ=60)
                  CAGGACCGCTTCGCTATTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTC                                                                         >  SRR4302534.4340165/1‑94 (MQ=60)
                  CAGGACCGCTTCGCTATTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTA                                                                         <  SRR4302534.2104802/94‑1 (MQ=60)
                  CAGGACCGCTTCGCTATTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGGGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTA                                                                         >  SRR4302534.3705877/1‑94 (MQ=60)
                   AGGACCGCTTCGCTATTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTAC                                                                        >  SRR4302534.3113970/1‑94 (MQ=60)
                   AGGACCGCTTCGCTATTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTAC                                                                        >  SRR4302534.2440143/1‑94 (MQ=60)
                   AGGACCGCTTCGCTATTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTAC                                                                        >  SRR4302534.3258371/1‑94 (MQ=60)
                   AGGACCGCTTCGCTATTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTAC                                                                        >  SRR4302534.4858274/1‑94 (MQ=60)
                    GGACCGCTTCGCTATTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACA                                                                       >  SRR4302534.2977411/1‑94 (MQ=60)
                    GGACCGCTTCGCTATTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACA                                                                       <  SRR4302534.4797353/94‑1 (MQ=60)
                    GGACCGCTTCGCTATTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACA                                                                       >  SRR4302534.5195124/1‑94 (MQ=60)
                     GACCGCTTCGCTATTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAA                                                                      >  SRR4302534.4414764/1‑94 (MQ=60)
                     GACCGCTTCGCTATTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAA                                                                      >  SRR4302534.2213985/1‑94 (MQ=60)
                     GACCGCTTCGCTATTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAA                                                                      >  SRR4302534.5496676/1‑94 (MQ=60)
                      ACCGCTTCGCTATTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAAC                                                                     >  SRR4302534.572516/1‑94 (MQ=60)
                        CTCTTCGCTATTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAG                                                                   >  SRR4302534.6370337/1‑94 (MQ=60)
                        CGCTTCGCTATTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAG                                                                   >  SRR4302534.959954/1‑94 (MQ=60)
                        CGCTTCGCTATTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAG                                                                   <  SRR4302534.4833269/94‑1 (MQ=60)
                        CGCTTCGCTATTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAG                                                                   >  SRR4302534.5641211/1‑94 (MQ=60)
                        CGCTTCGCTATTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAG                                                                   >  SRR4302534.790723/1‑94 (MQ=60)
                         GCTTCGCTATTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGA                                                                  >  SRR4302534.4946047/1‑94 (MQ=60)
                          CTTCGCTATTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTA                                                                 >  SRR4302534.3131375/1‑94 (MQ=60)
                           TTCGCTATTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAA                                                                >  SRR4302534.4942056/1‑94 (MQ=60)
                           TTCGCTATTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAA                                                                <  SRR4302534.6028604/94‑1 (MQ=60)
                            TCGCTATTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAAC                                                               >  SRR4302534.3856400/1‑94 (MQ=60)
                             CGCTATTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTATCGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACG                                                              >  SRR4302534.5943648/1‑94 (MQ=60)
                             CGCTATTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACG                                                              >  SRR4302534.6388569/1‑94 (MQ=60)
                             CGCTATTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACG                                                              <  SRR4302534.5741959/94‑1 (MQ=60)
                             CGCTATTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACG                                                              >  SRR4302534.886509/1‑94 (MQ=60)
                             CGCTATTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACG                                                              >  SRR4302534.3660039/1‑94 (MQ=60)
                              GCTATTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGC                                                             >  SRR4302534.2585461/1‑94 (MQ=60)
                               CTATTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCC                                                            <  SRR4302534.708620/94‑1 (MQ=60)
                                TATTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCA                                                           >  SRR4302534.3191804/1‑94 (MQ=60)
                                TATTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCA                                                           >  SRR4302534.2933711/1‑94 (MQ=60)
                                TATTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCA                                                           <  SRR4302534.539779/94‑1 (MQ=60)
                                 ATTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCAC                                                          >  SRR4302534.5588740/1‑94 (MQ=60)
                                 ATTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCAC                                                          >  SRR4302534.1589999/1‑94 (MQ=60)
                                 ATTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCAC                                                          >  SRR4302534.5678063/1‑94 (MQ=60)
                                 ATTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCAC                                                          >  SRR4302534.3955704/1‑94 (MQ=60)
                                  TTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACA                                                         <  SRR4302534.5239090/94‑1 (MQ=60)
                                  TTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACA                                                         >  SRR4302534.3823587/1‑94 (MQ=60)
                                    GTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGC                                                       >  SRR4302534.6008506/1‑94 (MQ=60)
                                     TAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCA                                                      <  SRR4302534.5848276/94‑1 (MQ=60)
                                     TAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCA                                                      >  SRR4302534.4500077/1‑94 (MQ=60)
                                       GCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCAAG                                                    <  SRR4302534.853068/94‑1 (MQ=60)
                                         CGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCAAGTA                                                  >  SRR4302534.803573/1‑94 (MQ=60)
                                          GTAGGTCTGCGTCTGCCAAAAGAGTGGCACCCTGTGCTAACGTATGGCGACTTATCTCGTCTGGTTCCTACACCAGTAACGCCACAGCAAGTAT                                                 <  SRR4302534.4240167/94‑1 (MQ=60)
                                          GTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCAAGTAT                                                 <  SRR4302534.2273839/94‑1 (MQ=60)
                                          GTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCAAGTAT                                                 <  SRR4302534.3342577/94‑1 (MQ=60)
                                           TAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCAAGTATT                                                >  SRR4302534.149301/1‑94 (MQ=60)
                                           TAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCAAGTATT                                                <  SRR4302534.240493/94‑1 (MQ=60)
                                           TAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCAAGTATT                                                >  SRR4302534.6165178/1‑94 (MQ=60)
                                            AGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCAAGTATTT                                               >  SRR4302534.6330726/1‑94 (MQ=60)
                                               TCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCAAGTATTTAAT                                            <  SRR4302534.2232866/94‑1 (MQ=60)
                                               TCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCAAGTATTTAAT                                            >  SRR4302534.1002014/1‑94 (MQ=60)
                                                 TGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCAAGTATTTAATGC                                          >  SRR4302534.815480/1‑94 (MQ=60)
                                                 TGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCAAGTATTTAATGC                                          >  SRR4302534.1687547/1‑94 (MQ=60)
                                                 TGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCAAGTATTTAATGC                                          <  SRR4302534.2340769/94‑1 (MQ=60)
                                                 TGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCAAGTATTTAATGC                                          >  SRR4302534.56536/1‑94 (MQ=60)
                                                   CGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCAAGTATTTAATGCGG                                        >  SRR4302534.3721139/1‑94 (MQ=60)
                                                     TCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCAAGTATTTAATGCGGTG                                      <  SRR4302534.5834056/94‑1 (MQ=60)
                                                      CTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCAAGTATTTAATGCGGTGT                                     <  SRR4302534.1925256/94‑1 (MQ=60)
                                                      CTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCAAGTATTTAATGCGGTGT                                     <  SRR4302534.6538916/94‑1 (MQ=60)
                                                       TGCCAAAAGAGTGGCAACCTGTACTAACGTATGGTGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCAAGTATTTAATGCGGTGTG                                    >  SRR4302534.4392258/1‑94 (MQ=60)
                                                       TGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCAAGTATTTAATGCGGTGTG                                    >  SRR4302534.310071/1‑94 (MQ=60)
                                                       TGCCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCAAGTATTTAATGCGGTGTG                                    >  SRR4302534.831512/1‑94 (MQ=60)
                                                         CCAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCAAGTATTTAATGCGGTGTGTC                                  >  SRR4302534.183994/1‑94 (MQ=60)
                                                          CAAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCAAGTATTTAATGCGGTGTGTCA                                 <  SRR4302534.6535357/94‑1 (MQ=60)
                                                           AAAAGAGTGGCACCCGGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCAAGTATTTAATGCGGTGTGTCAT                                <  SRR4302534.4231902/94‑1 (MQ=60)
                                                           AAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCAAGTATTTAATGCGGTGTGTCAT                                >  SRR4302534.1246010/1‑94 (MQ=60)
                                                           AAAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCAAGTATTTAATGCGGTGTGTCAT                                <  SRR4302534.3452396/94‑1 (MQ=60)
                                                            AAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCAAGTATTTAATGCGGTGTGTCATA                               <  SRR4302534.446159/94‑1 (MQ=60)
                                                            AAAGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCAAGTATTTAATGCGGTGTGTCATA                               >  SRR4302534.1272826/1‑94 (MQ=60)
                                                              AGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCAAGTATTTAATGCGGTGTGTCATATG                             <  SRR4302534.3610810/94‑1 (MQ=60)
                                                              AGAGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCAAGTATTTAATGCGGTGTGTCATATG                             <  SRR4302534.6111821/94‑1 (MQ=60)
                                                                AGTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCAAGTATTTAATGCGGTGTGTCATATGCG                           >  SRR4302534.3592476/1‑94 (MQ=60)
                                                                 GTGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCAAGTATTTAATGCGGTGTGTCATATGCGC                          <  SRR4302534.4277155/94‑1 (MQ=60)
                                                                  TGGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCAAGTATTTAATGCGGTGTGTCATATGCGCA                         >  SRR4302534.6263688/1‑94 (MQ=60)
                                                                   GGCAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCAAGTATTTAATGCGGTGTGTCATATGCGCAC                        >  SRR4302534.4771095/1‑94 (MQ=60)
                                                                     CAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCAAGTATTTAATGCGGTGTGTCATATGCGCACCA                      >  SRR4302534.159741/1‑94 (MQ=60)
                                                                     CAACCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCAAGTATTTAATGCGGTGTGTCATATGCGCACCA                      >  SRR4302534.6147346/1‑94 (MQ=60)
                                                                        CCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCAAGTATTTAATGCGGTGTGTCATATGCGCACCACCA                   >  SRR4302534.5519601/1‑94 (MQ=60)
                                                                        CCTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCAAGTATTTAATGCGGTGTGTCATATGCGCACCACCA                   <  SRR4302534.2913380/94‑1 (MQ=60)
                                                                         CTGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCAAGTATTTAATGCGGTGTGTCATATGCGCACCACCAA                  <  SRR4302534.5736512/94‑1 (MQ=60)
                                                                          TGTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCAAGTATTTAATGCGGTGTGTCATATGCGCACCACCAAA                 >  SRR4302534.355950/1‑94 (MQ=60)
                                                                           GTACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCAAGTATTTAATGCGGTGTGTCATATGCGCACCACCAAAC                >  SRR4302534.3531116/1‑94 (MQ=60)
                                                                            TACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCAAGTATTTAATGCGGTGTGTCATATGCGCACCACCAAACT               <  SRR4302534.3507234/94‑1 (MQ=60)
                                                                             ACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCAAGTATTTAATGCGGTGTGTCATATGCGCACCACCAAACTC              >  SRR4302534.2520785/1‑94 (MQ=60)
                                                                             ACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCAAGTATTTAATGCGGTGTGTCATATGCGCACCACCAAACTC              <  SRR4302534.5777429/94‑1 (MQ=60)
                                                                             ACTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCAAGTATTTAATGCGGTGTGTCATATGCGCACCACCAAACTC              >  SRR4302534.1127483/1‑94 (MQ=60)
                                                                              CTAACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCAAGTATTTAATGCGGTGTGTCATATGCGCACCACCAAACTCC             >  SRR4302534.2311885/1‑94 (MQ=60)
                                                                                AACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCAAGTATTTAATGCGGTGTGTCATATGCGCACCACCAAACTCCCT           >  SRR4302534.1536593/1‑94 (MQ=60)
                                                                                AACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCAAGTATTTAATGCGGTGTGTCATATGCGCACCACCAAACTCCCT           <  SRR4302534.3469780/94‑1 (MQ=60)
                                                                                AACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCAAGTATTTAATGCGGTGTGTCATATGCGCACCACCAAACTCCCT           <  SRR4302534.301861/94‑1 (MQ=60)
                                                                                AACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCAAGTATTTAATGCGGTGTGTCATATGCGCACCACCAAACTCCCT           <  SRR4302534.6105432/94‑1 (MQ=60)
                                                                                AACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCAAGTATTTAATGCGGTGTGTCATATGCGCACCACCAAACTCCCT           <  SRR4302534.5001352/94‑1 (MQ=60)
                                                                                AACGTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCAAGTATTTAATGCGGTGTGTCATATGCGCACCACCAAACTCCCT           >  SRR4302534.5145651/1‑94 (MQ=60)
                                                                                   GTATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCAAGTATTTAATGCGGTGTGTCATATGCGCACCACCAAACTCCCTGAT        >  SRR4302534.2234744/1‑94 (MQ=60)
                                                                                    TATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCAAGTATTTAATGCGGTGTGTCATATGCGCACCACCAAACTCCCTGATC       <  SRR4302534.5180621/94‑1 (MQ=60)
                                                                                     ATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCAAGTATTTAATGCGGTGTGTCATATGCGCCCCACCAAACTCCCTGATCC      <  SRR4302534.2576155/94‑1 (MQ=60)
                                                                                     ATGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCAAGTATTTAATGCGGTGTGTCATATGCGCACCACCAAACTCACTGATCC      >  SRR4302534.5694813/1‑94 (MQ=60)
                                                                                      TGGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCAAGTATTTAATGCGGTGTGTCATATGCGCACCACCAAACTCCCTGATCCA     <  SRR4302534.4171542/94‑1 (MQ=60)
                                                                                       GGCGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCAAGTATTTAATGCGGTGTGTCATATGCGCACCACCAAACTCCCTGATCCAA    <  SRR4302534.720732/94‑1 (MQ=60)
                                                                                         CGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCAAGTATTTAATGCGGTGTGTCATATGCGCACCACCAAACTCCCTGATCCAAAA  >  SRR4302534.2908411/1‑94 (MQ=60)
                                                                                         |                                                                                             
ATTTTTAAACATGAATACCAGGACCGCTTCGCTATTGTAGCCGTAGGTCTGCGTCTGCCAAAAGAGTGGCAACCTGTACTAACGTATGGTGACTTAACTCGTCTGGATCCTACAACAGTAACGCCACAGCAAGTATTTAATGCGGTGTGTCATATGCGCACCACCAAACTCCCTGATCCAAAA  >  NC_000913/4170119‑4170301

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑