Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 884 257.2 12958910 96.9% 12557183 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,171,680 A→G V309A (GTA→GCA)  coaA ← pantothenate kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,171,6800AG100.0% 727.7 / NA 206V309A (GTA→GCA) coaApantothenate kinase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (117/89);  total (117/89)

Only 100 of 207 total aligned reads displayed.

GGGCGGTGAAATATCCCTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGATCTCT  >  NC_000913/4171590‑4171769
                                                                                          |                                                                                         
gggCGGTGAAATATCCCTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGcc                                                                                         >  1:5434940/1‑93 (MQ=255)
 ggCGGTGAAATATCCCTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCg                                                                                        >  1:1240427/1‑93 (MQ=255)
  gCGGTGAAATATCCCTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGc                                                                                       >  1:806043/1‑93 (MQ=255)
  gCGGTGAAATATCCCTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGc                                                                                       <  1:3966059/93‑1 (MQ=255)
    ggTGAAATATCCCTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCAt                                                                                     >  2:2391843/1‑93 (MQ=255)
     gTGAAATATCCCTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATg                                                                                    >  2:5729745/1‑93 (MQ=255)
      tGAAATATCCCTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGa                                                                                   >  1:5247171/1‑93 (MQ=255)
       gAAATATCCCTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGAt                                                                                  <  2:3576338/93‑1 (MQ=255)
       gAAATATCCCTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGAt                                                                                  <  2:4786961/93‑1 (MQ=255)
        aaaTATCCCTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGAtt                                                                                 >  1:2514442/1‑93 (MQ=255)
        aaaTATCCCTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGAtt                                                                                 >  1:2885650/1‑93 (MQ=255)
         aaTATCCCTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTa                                                                                <  2:3870585/93‑1 (MQ=255)
         aaTATCCCTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTa                                                                                <  2:4706710/93‑1 (MQ=255)
          atatCCCTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAg                                                                               >  2:6141607/1‑93 (MQ=255)
          atatCCCTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAg                                                                               >  1:59976/1‑93 (MQ=255)
           tatCCCTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGc                                                                              >  1:2700451/1‑93 (MQ=255)
             tCCCTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCAc                                                                            <  1:4791489/93‑1 (MQ=255)
              cccTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACt                                                                           <  2:1366515/93‑1 (MQ=255)
              cccTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACt                                                                           <  2:1584669/93‑1 (MQ=255)
               ccTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACtt                                                                          >  2:5265370/1‑93 (MQ=255)
                cTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACttt                                                                         >  1:497417/1‑93 (MQ=255)
                cTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACttt                                                                         <  2:1285529/93‑1 (MQ=255)
                 tGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACtttt                                                                        <  2:2100284/93‑1 (MQ=255)
                  gCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTATTCTGCCGCATGATTAGCACTTTTc                                                                       <  1:4452059/93‑1 (MQ=255)
                   cGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCg                                                                      <  1:313970/93‑1 (MQ=255)
                       gTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAg                                                                  <  1:431315/93‑1 (MQ=255)
                        tGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAgg                                                                 >  1:2278282/1‑93 (MQ=255)
                         gCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGa                                                                >  2:4326781/1‑93 (MQ=255)
                          cAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGAt                                                               <  2:6271860/93‑1 (MQ=255)
                           aGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGAtt                                                              >  2:1089812/1‑93 (MQ=255)
                            gAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTa                                                             >  1:3489558/1‑93 (MQ=255)
                            gAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTa                                                             >  1:3089145/1‑93 (MQ=255)
                             aaaaaTAAGAAAGGGGAGTTTTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTaa                                                            >  1:5344198/1‑93 (MQ=255)
                             aaaaaTAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTaa                                                            <  1:2908601/93‑1 (MQ=255)
                               aaaTAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAAc                                                          <  1:3052973/93‑1 (MQ=255)
                                aaTAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACt                                                         <  1:1033840/93‑1 (MQ=255)
                                 aTAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTg                                                        <  2:5506401/93‑1 (MQ=255)
                                 aTAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTg                                                        >  1:938166/1‑93 (MQ=255)
                                 aTAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGACTAAACTg                                                        >  1:4026405/1‑93 (MQ=255)
                                   aaGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGgc                                                      >  1:3617764/1‑93 (MQ=255)
                                   aaGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGgc                                                      >  1:1386783/1‑93 (MQ=255)
                                    aGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGtcg                                                     >  1:6209099/1‑93 (MQ=255)
                                    aGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGgcg                                                     >  1:5191634/1‑93 (MQ=255)
                                    aGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGgcg                                                     <  2:3734144/93‑1 (MQ=255)
                                     gAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGgcgc                                                    <  2:1751240/93‑1 (MQ=255)
                                      aaaGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGgcgcg                                                   >  2:4095422/1‑93 (MQ=255)
                                       aaGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGgcgcgc                                                  >  2:4689590/1‑93 (MQ=255)
                                       aaGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGgcgcgc                                                  <  2:6104033/93‑1 (MQ=255)
                                       aaGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGgcgcgc                                                  >  2:6455738/1‑93 (MQ=255)
                                        aGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCt                                                 <  2:649912/93‑1 (MQ=255)
                                         ggggAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTc                                                >  2:393458/1‑93 (MQ=255)
                                         ggggAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTc                                                <  1:3654106/93‑1 (MQ=255)
                                          gggAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCa                                               <  1:3672571/93‑1 (MQ=255)
                                           ggAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCAc                                              <  2:3412307/93‑1 (MQ=255)
                                           ggAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCAc                                              <  1:4669900/93‑1 (MQ=255)
                                            gAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACg                                             >  2:6185308/1‑93 (MQ=255)
                                            gAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACg                                             >  1:6282369/1‑93 (MQ=255)
                                             ggTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGa                                            <  2:4053225/92‑1 (MQ=255)
                                              gTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAg                                           >  1:1986208/1‑93 (MQ=255)
                                               tATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAgt                                          >  2:4248009/1‑93 (MQ=255)
                                               tATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAgt                                          >  2:5958887/1‑93 (MQ=255)
                                                aTTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAgta                                         <  2:3847499/93‑1 (MQ=255)
                                                aTTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAgta                                         <  2:1414263/93‑1 (MQ=255)
                                                   cGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAgtaggt                                      >  2:3820705/1‑93 (MQ=255)
                                                    gCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAgtaggta                                     >  2:3226228/1‑93 (MQ=255)
                                                    gCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAgtaggta                                     >  1:662874/1‑93 (MQ=255)
                                                    gCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAgtaggta                                     >  2:1817586/1‑93 (MQ=255)
                                                      tCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGa                                   <  1:4888124/93‑1 (MQ=255)
                                                       ccccTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGaa                                  >  1:436556/1‑93 (MQ=255)
                                                       ccccTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGaa                                  <  1:2403462/93‑1 (MQ=255)
                                                        cccTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAat                                 <  1:1555104/93‑1 (MQ=255)
                                                          cTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAatat                               >  1:1015915/1‑93 (MQ=255)
                                                           tGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATAtt                              <  1:1791822/93‑1 (MQ=255)
                                                             cAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATAtttt                            >  2:4730653/1‑93 (MQ=255)
                                                              aaaTTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTg                           >  2:779405/1‑93 (MQ=255)
                                                               aaTTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGc                          <  1:4709647/93‑1 (MQ=255)
                                                                attattTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGAAGGTAGAATATTTTGCt                         >  2:682590/1‑93 (MQ=255)
                                                                    tttGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTaa                     >  2:436988/1‑93 (MQ=255)
                                                                     ttGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAg                    <  2:4216492/93‑1 (MQ=255)
                                                                      tGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGt                   <  2:3094043/93‑1 (MQ=255)
                                                                       gCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGtt                  <  1:5579130/93‑1 (MQ=255)
                                                                        cGTAGTCTGCCCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTc                 <  2:2734847/93‑1 (MQ=255)
                                                                        cGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTc                 <  2:5429878/93‑1 (MQ=255)
                                                                         gTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCa                >  1:4539494/1‑93 (MQ=255)
                                                                          tAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAg               <  2:5655595/93‑1 (MQ=255)
                                                                            gTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGcc             <  2:3617764/93‑1 (MQ=255)
                                                                              cTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAg           <  2:1467533/93‑1 (MQ=255)
                                                                              cTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAg           <  1:1194330/93‑1 (MQ=255)
                                                                               tGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGt          >  2:5151267/1‑93 (MQ=255)
                                                                                gACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGtt         >  1:4751184/1‑93 (MQ=255)
                                                                                gACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGtt         <  1:4215792/93‑1 (MQ=255)
                                                                                 aCCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTg        >  1:4608156/1‑93 (MQ=255)
                                                                                  ccTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGa       <  2:4322497/93‑1 (MQ=255)
                                                                                  ccTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGa       >  2:3689519/1‑93 (MQ=255)
                                                                                   ctctTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGAt      >  2:280761/1‑93 (MQ=255)
                                                                                    tcttctGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGAtc     <  2:4471835/93‑1 (MQ=255)
                                                                                     cttctGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGAtct    <  1:1686756/93‑1 (MQ=255)
                                                                                      ttctGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGAtctc   <  2:1856433/93‑1 (MQ=255)
                                                                                      ttctGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGAtctc   <  2:1934674/93‑1 (MQ=255)
                                                                                      ttctGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGAtctc   >  2:2449670/1‑93 (MQ=255)
                                                                                          |                                                                                         
GGGCGGTGAAATATCCCTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGATCTCT  >  NC_000913/4171590‑4171769

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATGGGCGGTGAAATATCCCTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGATCTCTTTCC  >  NC_000913/4171588‑4171773
                                                                                            |                                                                                             
ATGGGCGGTGAAATATCCCTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGC                                                                                              >  SRR4302534.6015472/1‑94 (MQ=60)
 TGGGCGGTGAAATATCCCTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCC                                                                                             >  SRR4302534.5581288/1‑94 (MQ=60)
  GGGCGGTGAAATATCCCTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCG                                                                                            >  SRR4302534.3058840/1‑94 (MQ=60)
  GGGCGGTGAAATATCCCTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCG                                                                                            >  SRR4302534.1273119/1‑94 (MQ=60)
   GGCGGTGAAATATCCCTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGC                                                                                           >  SRR4302534.826295/1‑94 (MQ=60)
    GCGGTGAAATATCCCTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCA                                                                                          >  SRR4302534.2878269/1‑94 (MQ=60)
    GCGGTGAAATATCCCTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCA                                                                                          <  SRR4302534.4072224/94‑1 (MQ=60)
      GGTGAAATATCCCTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATG                                                                                        <  SRR4302534.6414570/94‑1 (MQ=60)
      GGTGAAATATCCCTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATG                                                                                        >  SRR4302534.5260811/1‑94 (MQ=60)
       GTGAAATATCCCTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGA                                                                                       >  SRR4302534.5388908/1‑94 (MQ=60)
       GTGAAATATCCCTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCGGCCGCATGA                                                                                       <  SRR4302534.6483807/94‑1 (MQ=60)
        TGAAATATCCCTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGAT                                                                                      <  SRR4302534.3439548/94‑1 (MQ=60)
         GAAATATCCCTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATT                                                                                     >  SRR4302534.1180133/1‑94 (MQ=60)
         GAAATATCCCTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATT                                                                                     >  SRR4302534.2961386/1‑94 (MQ=60)
         GAAATATCCCTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATT                                                                                     >  SRR4302534.2581203/1‑94 (MQ=60)
           AATATCCCTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAG                                                                                   >  SRR4302534.61598/1‑94 (MQ=60)
            ATATCCCTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGC                                                                                  >  SRR4302534.2771789/1‑94 (MQ=60)
             TATCCCTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCA                                                                                 >  SRR4302534.4841860/1‑94 (MQ=60)
               TCCCTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACT                                                                               <  SRR4302534.4921592/94‑1 (MQ=60)
                CCCTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTT                                                                              >  SRR4302534.495696/1‑94 (MQ=60)
                 CCTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTT                                                                             >  SRR4302534.509822/1‑94 (MQ=60)
                  CTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTT                                                                            >  SRR4302534.3733746/1‑94 (MQ=60)
                    GCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCG                                                                          <  SRR4302534.4690730/94‑1 (MQ=60)
                    GCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTATTCTGCCGCATGATTAGCACTTTTCG                                                                          <  SRR4302534.4572396/94‑1 (MQ=60)
                     CGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGT                                                                         <  SRR4302534.322065/94‑1 (MQ=60)
                       TAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCA                                                                       >  SRR4302534.5536203/1‑94 (MQ=60)
                         GTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGG                                                                     >  SRR4302534.2338736/1‑94 (MQ=60)
                         GTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGA                                                                     <  SRR4302534.442320/94‑1 (MQ=60)
                            CAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATT                                                                  >  SRR4302534.4152590/1‑94 (MQ=60)
                             AGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTA                                                                 >  SRR4302534.3170362/1‑94 (MQ=60)
                             AGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTA                                                                 <  SRR4302534.5383668/94‑1 (MQ=60)
                             AGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTA                                                                 >  SRR4302534.3583349/1‑94 (MQ=60)
                             AGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTA                                                                 >  SRR4302534.5919248/1‑94 (MQ=60)
                              GAAAAATAAGAAAGGGGAGTTTTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAA                                                                >  SRR4302534.5488284/1‑94 (MQ=60)
                               AAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAA                                                               <  SRR4302534.2984949/94‑1 (MQ=60)
                                 AAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACT                                                             <  SRR4302534.3133072/94‑1 (MQ=60)
                                  AATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTG                                                            >  SRR4302534.962030/1‑94 (MQ=60)
                                  AATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTG                                                            <  SRR4302534.1060514/94‑1 (MQ=60)
                                  AATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGACTAAACTG                                                            >  SRR4302534.4134371/1‑94 (MQ=60)
                                    TAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGC                                                          <  SRR4302534.4832545/94‑1 (MQ=60)
                                    TAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGC                                                          <  SRR4302534.2066444/94‑1 (MQ=60)
                                    TAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGC                                                          >  SRR4302534.1423277/1‑94 (MQ=60)
                                    TAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGC                                                          >  SRR4302534.3715028/1‑94 (MQ=60)
                                     AAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGTCG                                                         >  SRR4302534.6377880/1‑94 (MQ=60)
                                     AAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCG                                                         <  SRR4302534.4769587/94‑1 (MQ=60)
                                     AAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCG                                                         >  SRR4302534.5331593/1‑94 (MQ=60)
                                        AAAGGGGAGAATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGC                                                      >  SRR4302534.6320343/1‑94 (MQ=60)
                                           GGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCA                                                   <  SRR4302534.3752303/94‑1 (MQ=60)
                                            GGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCAC                                                  <  SRR4302534.3771300/94‑1 (MQ=60)
                                            GGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCAC                                                  <  SRR4302534.5389407/94‑1 (MQ=60)
                                             GGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACG                                                 <  SRR4302534.4796670/94‑1 (MQ=60)
                                             GGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACG                                                 >  SRR4302534.6452376/1‑94 (MQ=60)
                                             GGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACG                                                 >  SRR4302534.3770534/1‑94 (MQ=60)
                                              GAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGA                                                >  SRR4302534.4441866/1‑94 (MQ=60)
                                              GAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGA                                                >  SRR4302534.3705724/1‑94 (MQ=60)
                                               AGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAG                                               >  SRR4302534.2038217/1‑94 (MQ=60)
                                                GTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGT                                              <  SRR4302534.5635210/94‑1 (MQ=60)
                                                GTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGT                                              >  SRR4302534.2981231/1‑94 (MQ=60)
                                                 TATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTA                                             >  SRR4302534.3185133/1‑94 (MQ=60)
                                                 TATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTA                                             >  SRR4302534.942216/1‑94 (MQ=60)
                                                 TATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTA                                             <  SRR4302534.4090245/94‑1 (MQ=60)
                                                   TTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGG                                           <  SRR4302534.5052851/94‑1 (MQ=60)
                                                     CGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTA                                         >  SRR4302534.1879041/1‑94 (MQ=60)
                                                     CGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTA                                         >  SRR4302534.678997/1‑94 (MQ=60)
                                                      GCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAG                                        >  SRR4302534.4574581/1‑94 (MQ=60)
                                                        TCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAA                                      >  SRR4302534.447676/1‑94 (MQ=60)
                                                        TCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAA                                      <  SRR4302534.5020673/94‑1 (MQ=60)
                                                        TCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAA                                      >  SRR4302534.127146/1‑94 (MQ=60)
                                                         CCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAAT                                     <  SRR4302534.2467338/94‑1 (MQ=60)
                                                         CCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAAT                                     <  SRR4302534.3839112/94‑1 (MQ=60)
                                                          CCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATA                                    <  SRR4302534.1595828/94‑1 (MQ=60)
                                                           CCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATAT                                   >  SRR4302534.4381440/1‑94 (MQ=60)
                                                           CCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATAT                                   >  SRR4302534.1042066/1‑94 (MQ=60)
                                                           CCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATAT                                   <  SRR4302534.2487726/94‑1 (MQ=60)
                                                           CCTGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATAT                                   >  SRR4302534.269657/1‑94 (MQ=60)
                                                             TGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTT                                 <  SRR4302534.1838361/94‑1 (MQ=60)
                                                             TGCAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTT                                 >  SRR4302534.1049387/1‑94 (MQ=60)
                                                               CAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTG                               >  SRR4302534.1743028/1‑94 (MQ=60)
                                                               CAAATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTG                               >  SRR4302534.489414/1‑94 (MQ=60)
                                                                 AATTATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCT                             <  SRR4302534.4837510/94‑1 (MQ=60)
                                                                    TATTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTA                          <  SRR4302534.970476/94‑1 (MQ=60)
                                                                      TTTGCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAG                        <  SRR4302534.468717/94‑1 (MQ=60)
                                                                         GCGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTC                     <  SRR4302534.5729855/94‑1 (MQ=60)
                                                                          CGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCA                    >  SRR4302534.4662539/1‑94 (MQ=60)
                                                                          CGTAGTCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCA                    <  SRR4302534.1602663/94‑1 (MQ=60)
                                                                           GTAGTCTGCCCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAG                   <  SRR4302534.3146449/94‑1 (MQ=60)
                                                                               TCTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAG               <  SRR4302534.1563638/94‑1 (MQ=60)
                                                                                CTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGT              >  SRR4302534.6412971/1‑94 (MQ=60)
                                                                                CTGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGT              <  SRR4302534.1225746/94‑1 (MQ=60)
                                                                                 TGACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTT             >  SRR4302534.4880160/1‑94 (MQ=60)
                                                                                  GACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTG            <  SRR4302534.4329496/94‑1 (MQ=60)
                                                                                  GACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTG            >  SRR4302534.4733232/1‑94 (MQ=60)
                                                                                  GACCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGCAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTG            >  SRR4302534.4531378/1‑94 (MQ=60)
                                                                                    CCTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGAT          >  SRR4302534.3006356/1‑94 (MQ=60)
                                                                                     CTCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGATC         <  SRR4302534.2434467/94‑1 (MQ=60)
                                                                                      TCTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGATCT        >  SRR4302534.1386787/1‑94 (MQ=60)
                                                                                       CTTCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGATCTC       <  SRR4302534.1730620/94‑1 (MQ=60)
                                                                                         TCTGCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGATCTCTT     <  SRR4302534.7573/94‑1 (MQ=60)
                                                                                            TCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGATCTCTTTCC  <  SRR4302534.4328654/94‑1 (MQ=60)
                                                                                            GCCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGATCTCTTTCC  >  SRR4302534.4973120/1‑94 (MQ=60)
                                                                                            |                                                                                             
ATGGGCGGTGAAATATCCCTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGATCTCTTTCC  >  NC_000913/4171588‑4171773

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑