Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 884 257.2 12958910 96.9% 12557183 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,182,994 G→A A23T (GCT→ACT)  rpoC → RNA polymerase beta prime subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,182,9940GA99.2% 885.9 / NA 244A23T (GCT→ACT) rpoCRNA polymerase beta prime subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (131/110);  minor base C (0/2);  total (131/113)
Fisher's exact test for biased strand distribution p-value = 2.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 247 total aligned reads displayed.

GCTAACTCCGACGGGAGCAAATCCGTGAAAGATTTATTAAAGTTTCTGAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTGCTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCATCAACTACCGTACGTTCAAACCAGAA  >  NC_000913/4182904‑4183083
                                                                                          |                                                                                         
gCTAACTCCGACGGGAGCAAATCCGTGAAAGATTTATTAAAGTTTCTGAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTAct                                                                                         <  1:828694/93‑1 (MQ=255)
 cTAACTCCGACGGGAGCAAATCCGTGAAAGATTTATTAAAGTTTCTGAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTActc                                                                                        <  2:5702691/93‑1 (MQ=255)
  tAACTCCGACGGGAGCAAATCCGTGAAAGATTTATTAAAGTTTCTGAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGCTCAAAATTActct                                                                                       >  2:6286390/1‑93 (MQ=255)
  tAACTCCGACGGGAGCAAATCCGTGAAAGATTTATTAAAGTTTCTGAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTActct                                                                                       >  2:5810544/1‑93 (MQ=255)
  tAACTCCGACGGGAGCAAATCCGTGAAAGATTTATTAAAGTTTCTGAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTActct                                                                                       <  2:4332922/93‑1 (MQ=255)
  tAACTCCGACGGGAGCAAATCCGTGAAAGATTTATTAAAGTTTCTGAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTActct                                                                                       >  2:3597448/1‑93 (MQ=255)
  tAACTCCGACGGGAGCAAATCCGTGAAAGATTTATTAAAGTTTCTGAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTActct                                                                                       <  2:6248146/93‑1 (MQ=255)
   aaCTCCGACGGGAGCAAATCCGTGAAAGATTTATTAAAGTTTCTGAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTg                                                                                      >  2:2779961/1‑93 (MQ=255)
   aaCTCCGACGGGAGCAAATCCGTGAAAGATTTATTAAAGTTTCTGAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTg                                                                                      >  2:2263382/1‑93 (MQ=255)
   aaCTCCGACGGGAGCAAATCCGTGAAAGATTTATTAAAGTTTCTGAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTg                                                                                      >  2:1243303/1‑93 (MQ=255)
     cTCCGACGGGAGCAAATCCGTGAAAGATTTATTAAAGTTTCTGAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGc                                                                                    >  2:2952353/1‑93 (MQ=255)
      tCCGACGGGAGCAAATCCGTGACAGATTTATTAAAGTTTCTGAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCt                                                                                   <  2:2511657/93‑1 (MQ=255)
      tCCGACGGGAGCAAATCCGTGAAAGATTTATTAAAGTTTCTGAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCt                                                                                   >  1:4778674/1‑93 (MQ=255)
       ccGACGGGAGCAAATCCGTGAAAGATTTATTAAAGTTTCTGAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCtt                                                                                  <  2:758288/93‑1 (MQ=255)
         gACGGGAGCAAATCCGTGAAAGATTTATTAAAGTTTCTGAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCg                                                                                >  1:408184/1‑93 (MQ=255)
         gACGGGAGCAAATCCGTGAAAGATTTATTAAAGTTTCTGAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCg                                                                                <  1:4668456/93‑1 (MQ=255)
          aCGGGAGCAAATCCGTGAAAGATTTATTAAAGTTTCTGAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGc                                                                               >  2:791273/1‑93 (MQ=255)
          aCGGGAGCAAATCCGTGAAAGATTTATTAAAGTTTCTGAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGc                                                                               <  1:3003702/93‑1 (MQ=255)
           cGGGAGCAAATCCGTGAAAGATTTATTAAAGTTTCTGAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGcc                                                                              <  2:5475951/93‑1 (MQ=255)
            gggAGCAAATCCGTGAAAGATTTATTAAAGTTTCTGAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCa                                                                             >  1:6136305/1‑93 (MQ=255)
             ggAGCAAATCCGTGAAAGATTTATTAAAGTTTCTGAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAg                                                                            >  1:3678862/1‑93 (MQ=255)
             ggAGCAAATCCGTGAAAGATTTATTAAAGTTTCTGAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAg                                                                            >  1:2626624/1‑93 (MQ=255)
              gAGCAAATCCGTGAAAGATTTATTAAAGTTTCTGAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGa                                                                           >  2:3363908/1‑93 (MQ=255)
               aGCAAATCCGTGAAAGATTTATTAAAGTTTCTGAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGAc                                                                          <  1:5170568/93‑1 (MQ=255)
                 cAAATCCGTGAAAGATTTATTAAAGTTTCTGAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACAt                                                                        >  2:2373856/1‑93 (MQ=255)
                   aaTCCGTGAAAGATTTATTAAAGTTTCTGAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGa                                                                      <  2:2716263/93‑1 (MQ=255)
                   aaTCCGTGAAAGATTTATTAAAGTTTCTGAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGa                                                                      >  2:6431681/1‑93 (MQ=255)
                     tCCGTGAAAGATTTATTAAAGTTTCTGAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATc                                                                    >  2:613289/1‑93 (MQ=255)
                     tCCGTGAAAGATTTATTAAAGTTTCTGAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATc                                                                    <  1:4420045/93‑1 (MQ=255)
                       cGTGAAAGATTTATTAAAGTTTCTGAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCg                                                                  <  2:1910476/93‑1 (MQ=255)
                       cGTGAAAGATTTATTAAAGTTTCTGAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCg                                                                  >  1:6393166/1‑93 (MQ=255)
                        gTGAAAGATTTATTAAAGTTTCTGAAAGCGCAGACTAAAACCGAAGAGATTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATccct                                                                 >  2:1165036/1‑91 (MQ=255)
                         tGAAAGATTTATTAAAGTTTCTGAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGtt                                                                >  1:3156474/1‑93 (MQ=255)
                          gAAAGATTTATTAAAGTTTCTGAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTc                                                               >  2:531887/1‑93 (MQ=255)
                          gAAAGATTTATTAAAGTTTCTGAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTc                                                               >  1:72818/1‑93 (MQ=255)
                            aaGATTTATTAAAGTTTCTGAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCAt                                                             <  2:560870/93‑1 (MQ=255)
                            aaGATTTATTAAAGTTTCTGAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCAt                                                             <  2:593793/93‑1 (MQ=255)
                             aGATTTATTAAAGTTTCTGAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATg                                                            <  1:5691496/93‑1 (MQ=255)
                              gATTTATTAAAGTTTCTGAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATgg                                                           >  1:3745099/1‑93 (MQ=255)
                               aTTTATTAAAGTTTCTGAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGt                                                          <  2:3040214/93‑1 (MQ=255)
                                  tattaAAGTTTCTGAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCtt                                                       >  1:6201663/1‑93 (MQ=255)
                                  tattaAAGTTTCTGAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCtt                                                       <  1:6374827/93‑1 (MQ=255)
                                   attaAAGTTTCTGAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCttt                                                      <  2:4291551/93‑1 (MQ=255)
                                   attaAAGTTTCTGAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCttt                                                      <  2:3141167/93‑1 (MQ=255)
                                     taAAGTTTCTGAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCg                                                    <  1:4619047/93‑1 (MQ=255)
                                     taAAGTTTCTGAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCg                                                    <  1:134363/93‑1 (MQ=255)
                                         gTTTCTGAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGa                                                >  1:1785694/1‑93 (MQ=255)
                                         gTTTCTGAAAGAGCAGACTAAAACCGAAGAGGTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGa                                                >  2:4046956/1‑93 (MQ=255)
                                          tttCTGAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGaa                                               <  2:4764817/93‑1 (MQ=255)
                                          tttCTGAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGaa                                               <  2:2224380/93‑1 (MQ=255)
                                            tCTGAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGt                                             >  1:4000330/1‑93 (MQ=255)
                                             cTGAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGtt                                            <  2:3509675/93‑1 (MQ=255)
                                               gAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTaa                                          >  1:3515145/1‑93 (MQ=255)
                                               gAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTaa                                          >  1:2233986/1‑93 (MQ=255)
                                               gAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTaa                                          >  1:2021654/1‑93 (MQ=255)
                                                aaaGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTaaa                                         >  2:5372477/1‑93 (MQ=255)
                                                 aaGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTaaaa                                        <  2:4462482/93‑1 (MQ=255)
                                                    cgcAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGc                                     <  1:3950592/93‑1 (MQ=255)
                                                     gcAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGcc                                    <  1:5279450/93‑1 (MQ=255)
                                                     gcAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGcc                                    >  2:20299/1‑93 (MQ=255)
                                                      cAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCg                                   >  1:336908/1‑93 (MQ=255)
                                                       aGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCgg                                  >  2:4648684/1‑93 (MQ=255)
                                                       aGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCgg                                  >  2:296734/1‑93 (MQ=255)
                                                       aGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCgg                                  >  1:2339552/1‑93 (MQ=255)
                                                        gACTAAAGCCGAAGAGTTTGATGCTATCAAACTTACTCTGGCTGCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGa                                 <  1:2564094/93‑1 (MQ=255)
                                                        gACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGa                                 >  1:2429007/1‑93 (MQ=255)
                                                        gACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGa                                 <  1:2775360/93‑1 (MQ=255)
                                                           tAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAAc                              <  1:5956254/93‑1 (MQ=255)
                                                           caaaaCCCGAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAAc                              <  2:1964159/92‑1 (MQ=255)
                                                             aaaCCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCa                            <  2:2322667/93‑1 (MQ=255)
                                                              aaCCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCAt                           >  1:5549001/1‑93 (MQ=255)
                                                                ccGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCATCa                         >  2:5982494/1‑93 (MQ=255)
                                                                 cGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCATCaa                        >  2:3788577/1‑93 (MQ=255)
                                                                 cGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCATCaa                        >  1:4223910/1‑93 (MQ=255)
                                                                  gAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCATCAAc                       >  2:2292587/1‑93 (MQ=255)
                                                                  gAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCATCAAc                       >  1:3308534/1‑93 (MQ=255)
                                                                  gAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCATCAAc                       <  1:4335572/93‑1 (MQ=255)
                                                                   aaGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCATCAACt                      >  2:5433471/1‑93 (MQ=255)
                                                                   aaGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCATCAACt                      >  2:4595742/1‑93 (MQ=255)
                                                                     gagTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCATCAACTAc                    >  1:539276/1‑93 (MQ=255)
                                                                      agTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCATCAACTAcc                   <  2:4905239/93‑1 (MQ=255)
                                                                       gTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCATCAACTACCg                  >  2:3909029/1‑93 (MQ=255)
                                                                        tttGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCATCAACTACCGt                 >  1:2742005/1‑93 (MQ=255)
                                                                        tttGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCATCAACTACCGt                 <  2:2211218/93‑1 (MQ=255)
                                                                         ttGATGCGATCAAAATTTCTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCATCAACTACCGTa                <  2:1944344/93‑1 (MQ=255)
                                                                         ttGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCATCAACTACCGTa                <  1:5870692/93‑1 (MQ=255)
                                                                         ttGATGCGATAAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCATCAACTACCGTa                <  1:4906693/93‑1 (MQ=255)
                                                                           gATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCATCAACTACCGTACg              >  2:3820055/1‑93 (MQ=255)
                                                                           gATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCATCAACTACCGTACg              >  2:3119021/1‑93 (MQ=255)
                                                                            aTGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCATCAACTACCGTACGt             <  2:151116/93‑1 (MQ=255)
                                                                            aTGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCATCAACTACCGTACGt             >  1:5370121/1‑93 (MQ=255)
                                                                             tGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCATCAACTACCGTACGtt            >  2:210171/1‑93 (MQ=255)
                                                                              gCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCATCAACTACCGTACGTTc           >  1:1083337/1‑93 (MQ=255)
                                                                              gCGATCAAAATTACTCGGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCATCAACTACCGTACGTTc           <  2:5075667/93‑1 (MQ=255)
                                                                               cGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCATCAACTACCGTACGTTCa          >  2:1326860/1‑93 (MQ=255)
                                                                                 aTCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCATCAACTACCGTACGTTCaaa        >  2:2595043/1‑93 (MQ=255)
                                                                                 aTCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCATCAACTACCGTACGTTCaaa        >  1:2126395/1‑93 (MQ=255)
                                                                                    aaaaTTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCATCAACTACCGTACGTTCAAACCa     >  1:5673993/1‑93 (MQ=255)
                                                                                      aaTTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCATCAACTACCGTACGTTCAAACCAGa   <  1:1040392/93‑1 (MQ=255)
                                                                                       aTTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCATCAACTACCGTACGTTCAAACCAGaa  <  2:5857874/93‑1 (MQ=255)
                                                                                          |                                                                                         
GCTAACTCCGACGGGAGCAAATCCGTGAAAGATTTATTAAAGTTTCTGAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTGCTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCATCAACTACCGTACGTTCAAACCAGAA  >  NC_000913/4182904‑4183083

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTGCTAACTCCGACGGGAGCAAATCCGTGAAAGATTTATTAAAGTTTCTGAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTGCTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCATCAACTACCGTACGTTCAAACCAGAACGTG  >  NC_000913/4182902‑4183087
                                                                                            |                                                                                             
GTGCTAACTCCGACGGGAGCAAATCCGTGAAAGATTTATTAAAGTTTCTGAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTAC                                                                                              >  SRR4302534.3420839/1‑94 (MQ=60)
  GCTAACTCCGACGGGAGCAAATCCGTGAAAGATTTATTAAAGTTTCTGAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTC                                                                                            <  SRR4302534.849549/94‑1 (MQ=60)
      ACTCCGACGGGAGCAAATCCGTGAAAGATTTATTAAAGTTTCTGAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGC                                                                                        <  SRR4302534.3150456/94‑1 (MQ=60)
       CTCCGACGGGAGCAAATCCGTGAAAGATTTATTAAAGTTTCTGAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCT                                                                                       >  SRR4302534.4908445/1‑94 (MQ=60)
       CTCCGACGGGAGCAAATCCGTGAAAGATTTATTAAAGTTTCTGAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCT                                                                                       <  SRR4302534.5550983/94‑1 (MQ=60)
        TCCGACGGGAGCAAATCCGTGAAAGATTTATTAAAGTTTCTGAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTT                                                                                      <  SRR4302534.1834611/94‑1 (MQ=60)
        TCCGACGGGAGCAAATCCGTGAAAGATTTATTAAAGTTTCTGAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTT                                                                                      >  SRR4302534.4589505/1‑94 (MQ=60)
         CCGACGGGAGCAAATCCGTGAAAGATTTATTAAAGTTTCTGAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTC                                                                                     <  SRR4302534.4362945/94‑1 (MQ=60)
          CGACGGGAGCAAATCCGTGAAAGATTTATTAAAGTTTCTGAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCG                                                                                    >  SRR4302534.418618/1‑94 (MQ=60)
          CGACGGGAGCAAATCCGTGAAAGATTTATTAAAGTTTCTGAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCG                                                                                    <  SRR4302534.653426/94‑1 (MQ=60)
           GACGGGAGCAAATCCGTGAAAGATTTATTAAAGTTTCTGAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGC                                                                                   <  SRR4302534.4795191/94‑1 (MQ=60)
            ACGGGAGCAAATCCGTGAAAGATTTATTAAAGTTTCTGAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCC                                                                                  <  SRR4302534.3082279/94‑1 (MQ=60)
             CGGGAGCAAATCCGTGAAAGATTTATTAAAGTTTCTGAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCA                                                                                 >  SRR4302534.6303224/1‑94 (MQ=60)
              GGGAGCAAATCCGTGAAAGATTTATTAAAGTTTCTGAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAG                                                                                >  SRR4302534.3777770/1‑94 (MQ=60)
              GGGAGCAAATCCGTGAAAGATTTATTAAAGTTTCTGAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAG                                                                                >  SRR4302534.2696107/1‑94 (MQ=60)
                 AGCAAATCCGTGAAAGATTTATTAAAGTTTCTGAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACA                                                                             <  SRR4302534.5309937/94‑1 (MQ=60)
                   CAAATCCGTGAAAGATTTATTAAAGTTTCTGAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATG                                                                           <  SRR4302534.4542029/94‑1 (MQ=60)
                      ATCCGTGAAAGATTTATTAAAGTTTCTGAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATC                                                                        >  SRR4302534.6243471/1‑94 (MQ=60)
                      ATCCGTGAAAGATTTATTAAAGTTTCTGAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATC                                                                        >  SRR4302534.5885245/1‑94 (MQ=60)
                       TCCGTGAAAGATTTATTAAAGTTTCTGAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCC                                                                       <  SRR4302534.4539377/94‑1 (MQ=60)
                       TCCGTGAAAGATTTATTAAAGTTTCTGAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCC                                                                       <  SRR4302534.5246531/94‑1 (MQ=60)
                        CCGTGAAAGATTTATTAAAGTTTCTGAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCG                                                                      >  SRR4302534.6565833/1‑94 (MQ=60)
                        CCGTGAAAGATTTATTAAAGTTTCTGAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCG                                                                      <  SRR4302534.2323467/94‑1 (MQ=60)
                         CGTGAAAGATTTATTAAAGTTTCTGAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGT                                                                     <  SRR4302534.1135614/94‑1 (MQ=60)
                         CGTGAAAGATTTATTAAAGGTTCTGAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGT                                                                     >  SRR4302534.6053837/1‑94 (MQ=60)
                          GTGAAAGATTTATTAAAGTTTCTGAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTT                                                                    >  SRR4302534.3239611/1‑94 (MQ=60)
                          GTGAAAGATTTATTAAAGTTTCTGAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTAGCCAGACATGATCCGTT                                                                    >  SRR4302534.5985052/1‑94 (MQ=60)
                           TGAAAGATTTATTAAAGTTTCTGAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTC                                                                   >  SRR4302534.74803/1‑94 (MQ=60)
                           TGAAAGATTTATTAAAGTTTCTGAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTC                                                                   >  SRR4302534.1433930/1‑94 (MQ=60)
                               AGATTTATTAAAGTTTCTGAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGG                                                               >  SRR4302534.3845707/1‑94 (MQ=60)
                               AGATTTATTAAAGTTTCTGAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGG                                                               <  SRR4302534.5845644/94‑1 (MQ=60)
                                   TTATTAAAGTTTCTGAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTT                                                           <  SRR4302534.2810519/94‑1 (MQ=60)
                                   TTATTAAAGTTTCTGAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTT                                                           >  SRR4302534.6370330/1‑94 (MQ=60)
                                   TTATTAAAGTTTCTGAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTT                                                           <  SRR4302534.3970261/94‑1 (MQ=60)
                                    TATTAAAGTTTCTGAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTT                                                          <  SRR4302534.6546981/94‑1 (MQ=60)
                                     ATTAAAGTTTCTGAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTC                                                         <  SRR4302534.2122544/94‑1 (MQ=60)
                                      TTAAAGTTTCTGAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCG                                                        <  SRR4302534.2085742/94‑1 (MQ=60)
                                       TAAAGTTTCTGAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGG                                                       <  SRR4302534.137897/94‑1 (MQ=60)
                                       TAAAGTTTCTGAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGG                                                       <  SRR4302534.4744381/94‑1 (MQ=60)
                                        AAAGTTTCTGAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGT                                                      <  SRR4302534.4452084/94‑1 (MQ=60)
                                         AAGTTTCTGAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTG                                                     >  SRR4302534.3066910/1‑94 (MQ=60)
                                         AAGTTTCTGAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTG                                                     <  SRR4302534.5802124/94‑1 (MQ=60)
                                          AGTTTCTGAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGA                                                    <  SRR4302534.5237517/94‑1 (MQ=60)
                                          AGTTTCTGAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGA                                                    >  SRR4302534.1832057/1‑94 (MQ=60)
                                           GTTTCTGAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAA                                                   <  SRR4302534.3571717/94‑1 (MQ=60)
                                            TTTCTGAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAG                                                  <  SRR4302534.3736618/94‑1 (MQ=60)
                                             TTCTGAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGT                                                 >  SRR4302534.4107413/1‑94 (MQ=60)
                                              TCTGAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTT                                                <  SRR4302534.877697/94‑1 (MQ=60)
                                               CTGAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTA                                               <  SRR4302534.4506976/94‑1 (MQ=60)
                                                TGAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAA                                              >  SRR4302534.3609693/1‑94 (MQ=60)
                                                TGAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAA                                              >  SRR4302534.2293167/1‑94 (MQ=60)
                                                TGAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAA                                              >  SRR4302534.2074546/1‑94 (MQ=60)
                                                TGAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAA                                              >  SRR4302534.2903290/1‑94 (MQ=60)
                                                   AAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAA                                           >  SRR4302534.3677584/1‑94 (MQ=60)
                                                   AAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAA                                           >  SRR4302534.2695041/1‑94 (MQ=60)
                                                    AGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAG                                          >  SRR4302534.5376676/1‑94 (MQ=60)
                                                      CGCAGACTAAAAGCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCC                                        <  SRR4302534.800565/94‑1 (MQ=60)
                                                      CGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCC                                        <  SRR4302534.4056354/94‑1 (MQ=60)
                                                       GCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCG                                       <  SRR4302534.5422004/94‑1 (MQ=60)
                                                       GCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCG                                       >  SRR4302534.345536/1‑94 (MQ=60)
                                                        CAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCGGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGG                                      >  SRR4302534.5519716/1‑94 (MQ=60)
                                                        CAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGG                                      >  SRR4302534.2401640/1‑94 (MQ=60)
                                                         AGACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGA                                     >  SRR4302534.2493589/1‑94 (MQ=60)
                                                          GACTAAAGCCGAAGAGTTTGATGCTATCAAACTTACTCTGGCTGCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAA                                    <  SRR4302534.2632054/94‑1 (MQ=60)
                                                          GACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAA                                    <  SRR4302534.2848381/94‑1 (MQ=60)
                                                           ACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAA                                   >  SRR4302534.342774/1‑94 (MQ=60)
                                                           ACTAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAA                                   <  SRR4302534.5830059/94‑1 (MQ=60)
                                                             TAAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACC                                 <  SRR4302534.6117988/94‑1 (MQ=60)
                                                              AAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCA                                <  SRR4302534.3354630/94‑1 (MQ=60)
                                                              AAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCA                                >  SRR4302534.2305634/1‑94 (MQ=60)
                                                              AAAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCA                                >  SRR4302534.2448269/1‑94 (MQ=60)
                                                               AAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAGAAAGCCGGAAACCAT                               >  SRR4302534.5509911/1‑94 (MQ=60)
                                                               AAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCAT                               >  SRR4302534.4304947/1‑94 (MQ=60)
                                                               AAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCAT                               >  SRR4302534.4092764/1‑94 (MQ=60)
                                                               AAACCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCAT                               >  SRR4302534.5698778/1‑94 (MQ=60)
                                                                  CCGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCATCAA                            >  SRR4302534.4337764/1‑94 (MQ=60)
                                                                  CCGAAAAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCCTCAA                            >  SRR4302534.1817362/1‑94 (MQ=60)
                                                                   CGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCATCAAC                           >  SRR4302534.3396888/1‑94 (MQ=60)
                                                                   CGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCATCAAC                           >  SRR4302534.6419083/1‑94 (MQ=60)
                                                                   CGAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCATCAAC                           >  SRR4302534.3308181/1‑94 (MQ=60)
                                                                    GAAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCATCAACT                          <  SRR4302534.4452775/94‑1 (MQ=60)
                                                                     AAGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCATCAACTA                         >  SRR4302534.4127182/1‑94 (MQ=60)
                                                                      AGAGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCATCAACTAC                        >  SRR4302534.552633/1‑94 (MQ=60)
                                                                        AGTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCATCAACTACCG                      >  SRR4302534.6136423/1‑94 (MQ=60)
                                                                        AGTTTGATGCGATCAAAATTACTCTGGCTTCGCCACACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCATCAACTACCG                      >  SRR4302534.2553348/1‑94 (MQ=60)
                                                                         GTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCATCAACTACCGT                     <  SRR4302534.259579/94‑1 (MQ=60)
                                                                         GTTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCATCAACTACCGT                     >  SRR4302534.2814251/1‑94 (MQ=60)
                                                                          TTTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCATCAACTACCGTA                    <  SRR4302534.4972170/94‑1 (MQ=60)
                                                                           TTGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCATCAACTACCGTAC                   <  SRR4302534.6030094/94‑1 (MQ=60)
                                                                           TTGATGCGATAAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCATCAACTACCGTAC                   <  SRR4302534.5039699/94‑1 (MQ=60)
                                                                            TGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCATCAACTACCGTACG                  <  SRR4302534.6595541/94‑1 (MQ=60)
                                                                            TGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCATCAACTACCGTACG                  <  SRR4302534.6595542/94‑1 (MQ=60)
                                                                            TGATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCATCAACTACCGTACG                  <  SRR4302534.4108533/94‑1 (MQ=60)
                                                                             GATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCATCAACTACCGTACGT                 >  SRR4302534.5514834/1‑94 (MQ=60)
                                                                              ATGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCATCAACTACCGTACGTT                >  SRR4302534.925688/1‑94 (MQ=60)
                                                                               TGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCATCAACTACCGTACGTTC               >  SRR4302534.1111512/1‑94 (MQ=60)
                                                                               TGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCATCAACTACCGTACGTTC               >  SRR4302534.6352258/1‑94 (MQ=60)
                                                                               TGCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCATCAACTACCGTACGTTC               <  SRR4302534.3951988/94‑1 (MQ=60)
                                                                                GCGATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCATCAACTACCGTACGTTCA              <  SRR4302534.3825481/94‑1 (MQ=60)
                                                                                  GATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCATCAACTACCGTACGTTCAAA            >  SRR4302534.5476900/1‑94 (MQ=60)
                                                                                  GATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCATCAACTACCGTACGTTCAAA            >  SRR4302534.4831412/1‑94 (MQ=60)
                                                                                  GATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCATCAACTACCGTACGTTCAAA            >  SRR4302534.2182394/1‑94 (MQ=60)
                                                                                  GATCAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCATCAACTACCGTACGTTCAAA            >  SRR4302534.645148/1‑94 (MQ=60)
                                                                                     CAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCATCAACTACCGTACGTTCAAACCA         >  SRR4302534.5827607/1‑94 (MQ=60)
                                                                                     CAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCATCAACTACCGTACGTTCAAACCA         <  SRR4302534.4834632/94‑1 (MQ=60)
                                                                                     CAAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCATCAACTACCGTACGGTCAAACCA         >  SRR4302534.4771108/1‑94 (MQ=60)
                                                                                      AAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCATCAACTACCGTACGTTCAAACCAG        >  SRR4302534.571922/1‑94 (MQ=60)
                                                                                      AAAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCATCAACTACCGTACGTTCAAACCAG        >  SRR4302534.1068556/1‑94 (MQ=60)
                                                                                       AAATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCATCAACTACCGTACGTTCAAACCAGA       >  SRR4302534.2845522/1‑94 (MQ=60)
                                                                                        AATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCATCAACTACCGTACGTTCAAACCAGAA      >  SRR4302534.2716394/1‑94 (MQ=60)
                                                                                        AATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCATCAACTACCGTACGTTCAAACCAGAA      <  SRR4302534.2200673/94‑1 (MQ=60)
                                                                                        AATTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCATCAACTACCGTACGTTCAAACCAGAA      <  SRR4302534.1067245/94‑1 (MQ=60)
                                                                                          TTACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCATCAACTACCGTACGTTCAAACCAGAACG    <  SRR4302534.4634268/94‑1 (MQ=60)
                                                                                           TACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCATCAACTACCGTACGTTCAAACCAGAACGT   >  SRR4302534.1937179/1‑94 (MQ=60)
                                                                                           TACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCATCAACTACCGTACGTTCAAACCAGAACGT   >  SRR4302534.1835241/1‑94 (MQ=60)
                                                                                            ACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCATCAACTACCGTACGTTCAAACCAGAACGTG  >  SRR4302534.1631196/1‑94 (MQ=60)
                                                                                            ACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCATCAACTACCGTACGTTCAAACCAGAACGTG  <  SRR4302534.6094585/94‑1 (MQ=60)
                                                                                            ACTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCATCAACTACCGTACGTTCAAACCAGAACGTG  >  SRR4302534.6083144/1‑94 (MQ=60)
                                                                                            |                                                                                             
GTGCTAACTCCGACGGGAGCAAATCCGTGAAAGATTTATTAAAGTTTCTGAAAGCGCAGACTAAAACCGAAGAGTTTGATGCGATCAAAATTGCTCTGGCTTCGCCAGACATGATCCGTTCATGGTCTTTCGGTGAAGTTAAAAAGCCGGAAACCATCAACTACCGTACGTTCAAACCAGAACGTG  >  NC_000913/4182902‑4183087

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑