Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 884 257.2 12958910 96.9% 12557183 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,234,288 A→G T150A (ACC→GCC)  b4027 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,234,2880AG99.5% 744.0 / ‑4.8 219T150A (ACC→GCC) b4027hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (104/114);  total (104/115)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.77e-01

Only 100 of 223 total aligned reads displayed.

GATGGCGCAAGCTGGACGCGCACGATGGGCTGGACCGAGTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCACCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGTTCGCGTGCTGGACGAAGAAGTCTCCACCGACCAGGCGCGCTGGCATAACCGC  >  NC_000913/4234198‑4234377
                                                                                          |                                                                                         
taTGGCGCAAGCTGGACGCGCACGATGGGCTGGACCGAGTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGcc                                                                                         <  2:1066758/92‑1 (MQ=255)
gATGGCGCAAGCTGGACGCGCACGATGGGCTGGACCGAGTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGcc                                                                                         >  1:1289119/1‑93 (MQ=255)
gATGGCGCAAGCTGGACGCGCACGATGGGCTGGACCGAGTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGcc                                                                                         <  2:5074659/93‑1 (MQ=255)
 gtGGCGCAAGCTGGACGCGCACGATGGGCTGGACCGAGTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCg                                                                                        <  1:2562269/92‑1 (MQ=255)
 aTGGCGCAAGCTGGACGCGCACGATGGGCTGGACCGAGTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCg                                                                                        >  2:3317351/1‑93 (MQ=255)
  tGGCGCAAGCTGGACGCGCACGATGGGCTGGACCGAGTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGa                                                                                       <  1:1597563/93‑1 (MQ=255)
   ggCGCAAGCTGGACGCGCACGATGGGCTGGACCGAGTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGAt                                                                                      <  1:6337902/93‑1 (MQ=255)
   ggCGCAAGCTGGACGCGCACGATGGGCTGGACCGAGTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGAt                                                                                      <  2:3084238/93‑1 (MQ=255)
     cgcAAGCTGGACGCGCACGATGGGCTGGACCGAGTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATAc                                                                                    >  2:5268408/1‑93 (MQ=255)
      gcAAGCTGGACGCGCACGATGGGCTGGACCGAGTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATAcc                                                                                   >  2:6224913/1‑93 (MQ=255)
      gcAAGCTGGACGCGCACGATGGGCTGGACCGAGTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATAcc                                                                                   >  2:6320817/1‑93 (MQ=255)
      gcAAGCTGGACGCGCACGATGGGCTGGACCGAGTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATAcc                                                                                   <  2:2877211/93‑1 (MQ=255)
       cAAGCTGGACGCGCACGATGGGCTGGACCGAGTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCg                                                                                  <  1:4315062/93‑1 (MQ=255)
       cAAGCTGGACGCGCACGATGGGCTGGACCGAGTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCg                                                                                  <  2:5624182/93‑1 (MQ=255)
        aaGCTGGACGCGCACGATGGGCTGGACCGAGTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGt                                                                                 >  1:619856/1‑93 (MQ=255)
        aaGCTGGACGCGCACGATGGGCTGGACCGAGTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGt                                                                                 <  1:2415067/93‑1 (MQ=255)
         aGCTGGACGCGCACGATGGGCTGGACCGAGTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTc                                                                                <  1:213658/93‑1 (MQ=255)
         aGCTGGACGCGCACGATGGGCTGGACCGAGTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTc                                                                                >  1:1434764/1‑93 (MQ=255)
         aGCTGGACGCGCACGATGGGCTGGACCGAGTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTc                                                                                <  2:4719337/93‑1 (MQ=255)
          gCTGGACGCGCACGATGGGCTGGACCGAGTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCa                                                                               >  2:4215135/1‑93 (MQ=255)
            tGGACGCGCACGATGGGCTGGACCGAGTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCaaa                                                                             <  1:6429162/93‑1 (MQ=255)
             ggACGCGCACTATGGGCTGGACCGAGTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGGCGATACCGTCAAAg                                                                            >  1:4418069/1‑93 (MQ=255)
             ggACGCGCACGATGGGCTGGACCGAGTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAg                                                                            >  1:5592742/1‑93 (MQ=255)
              gACGCGCACGATGGGCTGGACCGAGTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAgc                                                                           >  2:2143772/1‑92 (MQ=255)
              gACGCGCACGATGGGCTGGACCGAGTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGt                                                                           <  1:1495411/93‑1 (MQ=255)
              gACGCGCACGATGGGCTGGACCGAGTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGt                                                                           <  1:5817284/93‑1 (MQ=255)
              gACGCGCACGATGGGCTGGACCGAGTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGt                                                                           >  1:4541887/1‑93 (MQ=255)
              gACGCGCACGATGGGCTGGACCGAGTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGt                                                                           >  1:1225923/1‑93 (MQ=255)
                     aCGATGGGCTGGACCGAGTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGc                                                                    <  1:6457094/93‑1 (MQ=255)
                      cGATGGGCTGGACCGAGTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCg                                                                   <  1:1175369/93‑1 (MQ=255)
                       gATGGGCTGGACCGAGTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGa                                                                  >  1:1844841/1‑93 (MQ=255)
                       gATGGGCTGGACCGAGTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGa                                                                  >  2:1793012/1‑93 (MQ=255)
                        aTGGGCTGGACCGAGTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTGAAAGTCGGCAGCGAt                                                                 >  1:2743116/1‑93 (MQ=255)
                        aTGGGCTGGACCGAGTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGAt                                                                 >  2:3760329/1‑93 (MQ=255)
                        aTGGGCTGGACCGAGTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGAt                                                                 <  2:207143/93‑1 (MQ=255)
                         tGGGCTGGACCGAGTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATg                                                                >  2:5404550/1‑93 (MQ=255)
                         tGGGCTGGACCGAGTACCAGCAGGTACGCGACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATg                                                                <  2:4237295/93‑1 (MQ=255)
                              tGGACCGAGTACCAGCAGGTACGCTACGCCGCCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAAcc                                                           <  1:1766736/93‑1 (MQ=255)
                              tGGACCGAGTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAAcc                                                           >  2:5960601/1‑93 (MQ=255)
                               ggACCGAGTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAAccc                                                          >  2:5543501/1‑93 (MQ=255)
                               ggACCGAGTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAAccc                                                          <  2:4780209/93‑1 (MQ=255)
                               ggACCGAGTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCACCGATACCGTCAAAGTCGGCAGCGATGAAAccc                                                          <  1:1302499/93‑1 (MQ=255)
                                gACCGAGTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAAcccc                                                         <  2:5455191/93‑1 (MQ=255)
                                  ccGAGTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCgg                                                       <  1:4825336/93‑1 (MQ=255)
                                  ccGAGTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCgg                                                       >  2:2741232/1‑93 (MQ=255)
                                  ccGAGTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCgg                                                       >  1:4650901/1‑93 (MQ=255)
                                  ccGAGTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCgg                                                       >  2:5040274/1‑93 (MQ=255)
                                   cGAGTACCAGCAGGTACGCTACGCCACCGCACNCTNAGNNTTCAAATGGGATGGNGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGt                                                      <  1:5084862/93‑1 (MQ=255)
                                    gAGTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGtt                                                     >  2:2535359/1‑93 (MQ=255)
                                    gAGTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGtt                                                     >  1:1064904/1‑93 (MQ=255)
                                     aGTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGTTc                                                    >  2:2700651/1‑93 (MQ=255)
                                      gTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGTTcg                                                   >  2:4030333/1‑93 (MQ=255)
                                      gTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGTTcg                                                   >  1:311460/1‑93 (MQ=255)
                                       tACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGTTcgc                                                  <  1:4600522/93‑1 (MQ=255)
                                          cagcagGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGTTCGCGTg                                               >  1:6247439/1‑93 (MQ=255)
                                           agcagGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGTTCGCGTGc                                              <  2:3912695/93‑1 (MQ=255)
                                           agcagGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGTTCGCGTGc                                              <  2:4492797/93‑1 (MQ=255)
                                            gcagGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGTTCGCGTGCt                                             >  1:5155839/1‑93 (MQ=255)
                                             cagGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGTTCGCGTGCTg                                            <  2:127200/93‑1 (MQ=255)
                                              agGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGTTCGCGTGCTgg                                           <  1:5418453/93‑1 (MQ=255)
                                              agGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGTTCGCGTGCTgg                                           >  1:1938452/1‑93 (MQ=255)
                                              agGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGCATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAACCCCCGGTTCGCGTGCTgg                                           <  2:3969680/93‑1 (MQ=255)
                                               ggTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGTTCGCGTGCTGGa                                          >  2:16392/1‑93 (MQ=255)
                                                   cgctacgcCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGTTCGCGTGCTGGACgaa                                      <  1:1448334/93‑1 (MQ=255)
                                                     ctacgcCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGTTCGCGTGCTGGACgaaga                                    <  2:4641729/93‑1 (MQ=255)
                                                      tacgcCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGTTCGCGTGCTGGACgaagaa                                   >  2:167573/1‑93 (MQ=255)
                                                       acgcCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGTTCGCGTGCTGGACGaagaag                                  >  2:4352558/1‑93 (MQ=255)
                                                       acgcCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGTTCGCGTGCTGGACGaagaag                                  >  1:4764866/1‑93 (MQ=255)
                                                        cgcCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGTTCGCGTGCTGGACGAAGAAGt                                 >  1:2053247/1‑93 (MQ=255)
                                                         gcCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGTTCGCGTGCTGGACGAAGAAGtc                                <  2:5832490/93‑1 (MQ=255)
                                                           cACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGTTCGCGTGCTGGACGAAGAAGtctc                              <  1:720072/93‑1 (MQ=255)
                                                                cACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGTTCGCGTGCTGGACGAAGAAGTCTCCACCg                         >  2:2373944/1‑93 (MQ=255)
                                                                cACGCCCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGTTCGCGTGCTGGACGAAGAAGTCTCCACCg                         <  2:4975431/93‑1 (MQ=255)
                                                                 aCGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGTTCGCGTGCTGGACGAAGAAGTCTCCACCgc                        >  2:4182091/1‑92 (MQ=255)
                                                                  cGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGTTCGCGTGCTGGACGAAGAAGTCTCCACCGAc                       >  2:3514213/1‑93 (MQ=255)
                                                                  cGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGTTCGCGTGCTGGACGAAGAAGTCTCCACCGAc                       <  1:2622679/93‑1 (MQ=255)
                                                                  cGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGTTCGCGTGCTGGACGAAGAAGTCTCCACCGAc                       <  1:1700350/93‑1 (MQ=255)
                                                                  cGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGTTCGCGTGCTGGACGAAGAAGTCTCCACCGAc                       <  1:2299473/93‑1 (MQ=255)
                                                                   gCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGTTCGCGTGCTGGACGAAGAAGTCTCCACCGAcc                      >  1:3432797/1‑93 (MQ=255)
                                                                   gCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGTTCGCGTGCTGGACGAAGAAGTCTCCACCGAcc                      <  1:5122082/93‑1 (MQ=255)
                                                                    cTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGTTCGCGTGCTGGACGAAGAAGTCTCCACccacca                     >  1:3328652/1‑93 (MQ=255)
                                                                     tCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAATCCCCGGTTCGCGTGCTGGACGAAGAAGTCTCCACCGACCAg                    <  2:2118126/93‑1 (MQ=255)
                                                                      cAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGTTCGCGTGCTGGACGAAGAAGTCTCCACCGACCAgg                   <  2:1905022/93‑1 (MQ=255)
                                                                        gTCTTCAAATGGGATGGCGCCGATCCCGTCAAAGTCGGCAGCGATGAAACCCCGGTTCGCGTGCTGGACGAAGAAGTCTCCACCGACCAGgcg                 <  1:3046608/93‑1 (MQ=255)
                                                                         tCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGTTCGCGTGCTGGACGAAGAAGTCTCCACCGACCAGgcgc                >  2:3163388/1‑93 (MQ=255)
                                                                           ttCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGTTCGCGTGCTGGACGAAGAAGTCTCCACCGACCAGgcgcgc              <  1:2500368/93‑1 (MQ=255)
                                                                           ttCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGTTCGCGTGCTGGACGAAGAAGTCTCCACCGACCAGgcgcgc              <  2:1348530/93‑1 (MQ=255)
                                                                               aaTGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGTTCGCGTGCTGGACGAAGAAGTCTCCACCGACCAGGCGCGCTGGc          >  1:9983/1‑93 (MQ=255)
                                                                               aaTGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGTTCGCGTGCTGGACGAAGAAGTCTCCACCGACCAGGCGCGCTGGc          >  2:1496130/1‑93 (MQ=255)
                                                                               aaTGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGTTCGCGTGCTGGACGAAGAAGTCTCCACCGACCAGGCGCGCTGGc          >  2:1034920/1‑93 (MQ=255)
                                                                                aTGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGTTCGCGTGCTGGACGAAGAAGTCTCCACCGACCAGGCGCGCTGGCa         >  1:3740663/1‑93 (MQ=255)
                                                                                 tGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGTTCGCGTGCTGGACGAAGAAGTCTCCACCGACCAGGCGCGCTGGCAt        >  2:1411652/1‑93 (MQ=255)
                                                                                   ggATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGTTCGCGTGCTGGACGAAGAAGTCTCCACCGACCAGGCGCGCTGGCATaa      >  1:5470814/1‑93 (MQ=255)
                                                                                    gATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGTTCGCGTGCTGGACGAAGAAGTCTCCACCGACCAGGGGCGCTGGCATAAc     >  1:1734142/1‑93 (MQ=255)
                                                                                    gATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGTTCGCGTGCTGGACGAAGAAGTCTCCACCGACCAGGCGCGCTGGCATAAc     <  1:4152721/93‑1 (MQ=255)
                                                                                     aTGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGTTCGCGTGCTGGACGAAGAAGTCTCCACCGACCAGGCGCGCTGGCATAAcc    <  2:3332115/93‑1 (MQ=255)
                                                                                     aTGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGTTCGCGTGCTGGACGAAGAAGTCTCCACCGACCAGGCGCGCTGGCATAAcc    >  2:2209097/1‑93 (MQ=255)
                                                                                      tGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGTTCGCGTGCTGGACGAAGAAGTCTCCACCGACCAGGCGCGCTGGCATAACCg   >  1:2865383/1‑93 (MQ=255)
                                                                                       ggCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGTTCGCGTGCTGGACGAAGAAGTCTCCACCGACCAGGCGCGCTGGCATAACCGc  <  1:3592878/93‑1 (MQ=255)
                                                                                       ggCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGTTCGCGTGCTGGACGAAGAAGTCTCCACCGACCAGGCGCGCTGGCATAACCGc  >  2:645911/1‑93 (MQ=255)
                                                                                          |                                                                                         
GATGGCGCAAGCTGGACGCGCACGATGGGCTGGACCGAGTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCACCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGTTCGCGTGCTGGACGAAGAAGTCTCCACCGACCAGGCGCGCTGGCATAACCGC  >  NC_000913/4234198‑4234377

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTTGATGGCGCAAGCTGGACGCGCACGATGGGCTGGACCGAGTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCACCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGTTCGCGTGCTGGACGAAGAAGTCTCCACCGACCAGGCGCGCTGGCATAACCGCTATT  >  NC_000913/4234195‑4234381
                                                                                             |                                                                                             
GTTGATGGCGCAAGCTGGACGCGCACGATGGGCTGGACCGAGTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCG                                                                                               >  SRR4302534.2717141/1‑94 (MQ=60)
GTTGATGGCGCAAGCTGGACGCGCACGATGGGCTGGACCGAGTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCG                                                                                               >  SRR4302534.5939425/1‑94 (MQ=60)
  TGATGGCGCAAGCTGGACGCGCACGATGGGCTGGACCGAGTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCC                                                                                             >  SRR4302534.1323064/1‑94 (MQ=60)
  TGATGGCGCAAGCTGGACGCGCACGATGGGCTGGACCGAGTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCC                                                                                             <  SRR4302534.1361593/94‑1 (MQ=60)
   GATGGCGCAAGCTGGACGCGCACGATGGGCTGGACCGAGTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCG                                                                                            <  SRR4302534.2694927/94‑1 (MQ=60)
    GTGGCGCAAGCTGGACGCGCACGATGGGCTGGACCGAGTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGA                                                                                           <  SRR4302534.2630176/94‑1 (MQ=60)
    GTGGCGCAAGCTGAACGCGCACGATGGGCTGGACCGAGTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGA                                                                                           <  SRR4302534.1408558/94‑1 (MQ=60)
    ATGGCGCAAGCTGGACGCGCACGATGGGCTGGACCGAGTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGA                                                                                           <  SRR4302534.1743620/94‑1 (MQ=60)
    ATGGCGCAAGCTGGACGCGCACGATGGGCTGGACCGAGTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTATTCAAATGGGATGGCGCCGA                                                                                           <  SRR4302534.1177181/94‑1 (MQ=60)
     TGGCGCAAGCTGGACGCGCACGATGGGCTGGACCGAGTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGAT                                                                                          <  SRR4302534.1639308/94‑1 (MQ=60)
      GGCGCAAGCTGGACGCGCACGATGGGCTGGACCGAGTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATA                                                                                         <  SRR4302534.4989589/94‑1 (MQ=60)
      GGCGCAAGCTGGACGCGCACGATGGGCTGGACCGAGTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATA                                                                                         <  SRR4302534.6509106/94‑1 (MQ=60)
        CGCAAGCTGGACGCGCACGATGGGCTGGACCGAGTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACC                                                                                       <  SRR4302534.4699486/94‑1 (MQ=60)
         GCAAGCTGGACGCGCACGATGGGCTGGACCGAGTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCG                                                                                      <  SRR4302534.4100987/94‑1 (MQ=60)
          CAAGCTGGACGCGCACGATGGGCTGGACCGAGTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGT                                                                                     >  SRR4302534.634904/1‑94 (MQ=60)
          CAAGCTGGACGCGCACGATGGGCTGGACCGAGTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGT                                                                                     <  SRR4302534.4431928/94‑1 (MQ=60)
           AAGCTGGACGCGCACGATGGGCTGGACCGAGTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTC                                                                                    <  SRR4302534.2479298/94‑1 (MQ=60)
           AAGCTGGACGCGCACGATGGGCTGGACCGAGTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTC                                                                                    <  SRR4302534.6619461/94‑1 (MQ=60)
           AAGCTGGACGCGCACGATGGGCTGGACCGAGTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTC                                                                                    >  SRR4302534.1472515/1‑94 (MQ=60)
           AAGCTGGACGCGCACGATGGGCTGGACCGAGTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTC                                                                                    >  SRR4302534.1285450/1‑94 (MQ=60)
            AGCTGGACGCGCACGATGGGCTGGACCGAGTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCA                                                                                   <  SRR4302534.219257/94‑1 (MQ=60)
               TGGACGCGCACTATGGGCTGGACCGAGTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGGCGATACCGTCAAAG                                                                                >  SRR4302534.4537348/1‑94 (MQ=60)
               TGGACGCGCACGATGGGCTGGACCGAGTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAG                                                                                >  SRR4302534.4938003/1‑94 (MQ=60)
               TGGACGCGCACGATGGGCTGGACCGAGTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAG                                                                                <  SRR4302534.6602512/94‑1 (MQ=60)
               TGGACGCGCACGATGGGCTGGACCGAGTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAG                                                                                >  SRR4302534.4726486/1‑94 (MQ=60)
               TGGACGCGCACGATGGGCTGGACCGAGTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAG                                                                                >  SRR4302534.5743940/1‑94 (MQ=60)
                GGACGCGCACGATGGGCTGGACCGAGTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGT                                                                               >  SRR4302534.1258201/1‑94 (MQ=60)
                GGACGCGCACGATGGGCTGGACCGAGTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGT                                                                               >  SRR4302534.4665009/1‑94 (MQ=60)
                 GACGCGCACGATGGGCTGGACCGAGTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTC                                                                              <  SRR4302534.5975176/94‑1 (MQ=60)
                 GACGCGCACGATGGGCTGGACCGAGTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTC                                                                              <  SRR4302534.1534754/94‑1 (MQ=60)
                      GCACGATGGGCTGGACCGAGTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAG                                                                         <  SRR4302534.4851715/94‑1 (MQ=60)
                       CACGATGGGCTGGACCGAGTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGC                                                                        >  SRR4302534.2863842/1‑94 (MQ=60)
                       CACGATGGGCTGGACCGAGTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGC                                                                        <  SRR4302534.6510843/94‑1 (MQ=60)
                        ACGATGGGCTGGACCGAGTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCT                                                                       <  SRR4302534.6631054/94‑1 (MQ=60)
                        ACGATGGGCTGGACCGAGTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCT                                                                       <  SRR4302534.6631055/94‑1 (MQ=60)
                        ACGATGGGCTGGACCGAGTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCG                                                                       <  SRR4302534.6607782/94‑1 (MQ=60)
                         CGATGGGCTGGACCGAGTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGA                                                                      >  SRR4302534.1892824/1‑94 (MQ=60)
                         CGATGGGCTGGACCGAGTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGA                                                                      <  SRR4302534.1206268/94‑1 (MQ=60)
                          GATGGGCTGGACCGAGTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTGAAAGTCGGCAGCGAT                                                                     >  SRR4302534.2815383/1‑94 (MQ=60)
                          GATGGGCTGGACCGAGTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGAT                                                                     >  SRR4302534.3521631/1‑94 (MQ=60)
                          GATGGGCTGGACCGAGTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGAT                                                                     <  SRR4302534.5323432/94‑1 (MQ=60)
                          GATGGGCTGGACCGAGTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGAT                                                                     >  SRR4302534.5602369/1‑94 (MQ=60)
                             GGGCTGGACCGAGTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAA                                                                  >  SRR4302534.3779790/1‑94 (MQ=60)
                                CTGGACCGAGTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACC                                                               >  SRR4302534.6388915/1‑94 (MQ=60)
                                CTGGACCGAGTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACC                                                               >  SRR4302534.1767805/1‑94 (MQ=60)
                                 TGGACCGAGTACCAGCAGGTACGCTACGCCGCCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCC                                                              <  SRR4302534.1812618/94‑1 (MQ=60)
                                 TGGACCGAGTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACGC                                                              >  SRR4302534.3386110/1‑94 (MQ=60)
                                 TGGACCGAGTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCC                                                              >  SRR4302534.3632143/1‑94 (MQ=60)
                                  GGACCGAGTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCACCGATACCGTCAAAGTCGGCAGCGATGAAACCCC                                                             <  SRR4302534.1336852/94‑1 (MQ=60)
                                   GACCGAGTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATAGCGTCAAAGTTCGCAGCGATGGAACCGCG                                                            >  SRR4302534.3018414/1‑94 (MQ=60)
                                    ACCGAGTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGG                                                           >  SRR4302534.4522656/1‑94 (MQ=60)
                                    ACCGAGTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGG                                                           >  SRR4302534.4777172/1‑94 (MQ=60)
                                    ACCGAGTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGG                                                           <  SRR4302534.1672663/94‑1 (MQ=60)
                                    ACCGAGTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGG                                                           <  SRR4302534.1332291/94‑1 (MQ=60)
                                     CCGAGTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGT                                                          <  SRR4302534.4956281/94‑1 (MQ=60)
                                      CGAGTACCAGCAGGTACGCTACGCCACCGCACNCTNAGNNTTCAAATGGGATGGNGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGTT                                                         <  SRR4302534.5222109/94‑1 (MQ=60)
                                      CGAGTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGTT                                                         <  SRR4302534.909252/94‑1 (MQ=60)
                                      CGAGTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGTT                                                         >  SRR4302534.1092512/1‑94 (MQ=60)
                                        AGTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGTTCG                                                       <  SRR4302534.3145146/94‑1 (MQ=60)
                                        AGTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGTTCG                                                       >  SRR4302534.319496/1‑94 (MQ=60)
                                          TACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGTTCGCG                                                     <  SRR4302534.4725385/94‑1 (MQ=60)
                                           ACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGTTCGCGT                                                    <  SRR4302534.2168738/94‑1 (MQ=60)
                                            CCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGTTCGCGTG                                                   >  SRR4302534.6416875/1‑94 (MQ=60)
                                              AGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGTTCGCGTGCT                                                 >  SRR4302534.5294877/1‑94 (MQ=60)
                                              AGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGTTCGCGTGCT                                                 >  SRR4302534.1924362/1‑94 (MQ=60)
                                               GCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGTTCGCGTGCTG                                                <  SRR4302534.1350298/94‑1 (MQ=60)
                                                CAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGTTCGCGTGCTGG                                               >  SRR4302534.1989141/1‑94 (MQ=60)
                                                 AGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGTTCGCGTGCTGGA                                              <  SRR4302534.5564240/94‑1 (MQ=60)
                                                 AGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGTTCGCGTGCTGGA                                              >  SRR4302534.4454294/1‑94 (MQ=60)
                                                 AGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAAACCCGGTTCGCGTGCTCGA                                              >  SRR4302534.2030691/1‑94 (MQ=60)
                                                 AGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACAGTCAAAGTCGGCAGCGATGAAACCCCGGTTCGCGTGCTGGA                                              <  SRR4302534.5555801/94‑1 (MQ=60)
                                                      CGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGTTCGCGTGCTGGACGAAG                                         >  SRR4302534.6418837/1‑94 (MQ=60)
                                                      CGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGTTCGCGTGCTGGACGAAG                                         <  SRR4302534.1486452/94‑1 (MQ=60)
                                                        CTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGTTCGCGTGCTGGACGAAGAA                                       >  SRR4302534.3651977/1‑94 (MQ=60)
                                                         TACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGTTCGCGTGCTGGACGAAGAAG                                      >  SRR4302534.4894249/1‑94 (MQ=60)
                                                         TACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGTTCGCGTGCTGGACGAAGAAG                                      <  SRR4302534.2050149/94‑1 (MQ=60)
                                                          ACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGTTCGCGTGCTGGACGAAGAAGT                                     >  SRR4302534.2106897/1‑94 (MQ=60)
                                                          ACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGTTCGCGTGCTGGACGAAGAAGT                                     <  SRR4302534.6178942/94‑1 (MQ=60)
                                                             CCACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGTTCGCGTGCTGGACGAAGAAGTCTC                                  >  SRR4302534.1620652/1‑94 (MQ=60)
                                                              CACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGTTCGCGTGCTGGACGAAGAAGTCTCC                                 <  SRR4302534.737836/94‑1 (MQ=60)
                                                               ACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGTTCGCGTGCTGGACGAAGAAGTCTCCA                                <  SRR4302534.1478373/94‑1 (MQ=60)
                                                               ACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGTTCGCGTGCTGGACGAAGAAGTCTCCA                                <  SRR4302534.6240156/94‑1 (MQ=60)
                                                               ACCGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGTTCGCGTGCTGGACGAAGAAGTCTCCA                                <  SRR4302534.1533970/94‑1 (MQ=60)
                                                                 CGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGTTCGCGTGCTGGACGAAGAAGTCTCCACC                              <  SRR4302534.4339349/94‑1 (MQ=60)
                                                                 CGCACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGTTCGCGTGCTGGACGAAGAAGTCTCCACC                              >  SRR4302534.5281665/1‑94 (MQ=60)
                                                                   CACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGTTCGCGTGCTGGACGAAGAAGTCTCCACCGA                            <  SRR4302534.2642724/94‑1 (MQ=60)
                                                                    ACGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGTTCGCGTGCTGGACGAAGAAGTCTCCACCGAC                           <  SRR4302534.1222434/94‑1 (MQ=60)
                                                                     CGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGTTCGCGTGCTGGACGAAGAAGTCTCCACCGACC                          >  SRR4302534.3525026/1‑94 (MQ=60)
                                                                     CGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGTTCGCGTGCTGGACGAAGAAGTCTCCACCGACC                          <  SRR4302534.2360511/94‑1 (MQ=60)
                                                                     CGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGTTCGCGTGCTGGACGAAGAAGTCTCCACCGACC                          <  SRR4302534.1744529/94‑1 (MQ=60)
                                                                     CGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGTTCGCGTGCTGGACGAAGAAGTCTCCACCGACC                          <  SRR4302534.2692064/94‑1 (MQ=60)
                                                                     CGCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGGTCGCGTGCTGGACGAAGAAGTCTCCACCGACC                          >  SRR4302534.4556784/1‑94 (MQ=60)
                                                                      GCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGTTCGCGTGCTGGACGAAGAAGTCTCCACCGACCA                         <  SRR4302534.5260390/94‑1 (MQ=60)
                                                                      GCTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGTTCGCGTGCTGGACGAAGAAGTCTCCACCCACCA                         >  SRR4302534.3417677/1‑94 (MQ=60)
                                                                       CTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGTTCGCGTGCTGGACGAAGAAGTCTCCACCGACCAG                        <  SRR4302534.5737272/94‑1 (MQ=60)
                                                                       CTCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGTTCGCGTGCTGGACGAAGAAGTCTCCACCGACCAG                        <  SRR4302534.2539131/94‑1 (MQ=60)
                                                                        TCAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGTTCGCGTGCTGGACGAAGAAGTCTCCACCGACCAGG                       >  SRR4302534.1807301/1‑94 (MQ=60)
                                                                         CAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGTTCGCGTGCTGGACGAAGAAGTCTCCACCGACCAGGC                      <  SRR4302534.4858553/94‑1 (MQ=60)
                                                                         CAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGTTCGCGTGCTGGACGAAGAAGTCTCCACCGACCAGGC                      >  SRR4302534.2408111/1‑94 (MQ=60)
                                                                         CAGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGTTCGCGTGCTGGACGAAGAAGTCTCCACCGACCAGGC                      <  SRR4302534.2904396/94‑1 (MQ=60)
                                                                          AGTCTTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGTTCGCGTGCTGGACGAAGAAGTCTCCACCGACCAGGCG                     <  SRR4302534.1849537/94‑1 (MQ=60)
                                                                           GTCTTCAAATGGGATGGCGCCGATCCCGTCAAAGTCGGCAGCGATGAAACCCCGGTTCGCGTGCTGGACGAAGAAGTCTCCACCGACCAGGCGC                    <  SRR4302534.3126520/94‑1 (MQ=60)
                                                                              TTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAACCCCCGGTTCGCGTGCTGGACGAAGAAGTCTCCACCGACCAGGCGCGCT                 <  SRR4302534.2620454/94‑1 (MQ=60)
                                                                              TTCAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGTTCGCGTGCTGGACGAAGAAGTCTCCACCGACCAGGCGCGCT                 <  SRR4302534.2566754/94‑1 (MQ=60)
                                                                                CAAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGTTCGCGTGCTGGACGAAGAAGTCTCCACCGACCAGGCGCGCTGG               >  SRR4302534.5249618/1‑94 (MQ=60)
                                                                                CAAATGGGATGCCGCCGATACCGACAAAGTCGGCAGCGATGAAACCCCGGTTCGCGTGCTGGACGAAGAAGTCTCAACCGACCACGCGCGCTGG               >  SRR4302534.2351484/1‑94 (MQ=60)
                                                                                 AAATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGTTCGCGTGCTGGACGAAGAAGTCTCCACCGACCAGGCGCGCTGGC              >  SRR4302534.10241/1‑94 (MQ=60)
                                                                                  AATGGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGTTCGCGTGCTGGACGAAGAAGTCTCCACCGACCAGGCGCGCTGGCA             >  SRR4302534.3841166/1‑94 (MQ=60)
                                                                                     GGGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGTTCGCGTGCTGGACGAAGAAGTCTCCACCGACCAGGCGCGCTGGCATAA          >  SRR4302534.5618301/1‑94 (MQ=60)
                                                                                      GGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGTTCGCGTGCTGGACGAAGAAGTCTCCACCGACCAGGGGCGCTGGCATAAC         >  SRR4302534.1779181/1‑94 (MQ=60)
                                                                                      GGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGTTCGCGTGCTGGACGAAGAAGTCTCCACCGACCAGGCGCGCTGGCATAAC         >  SRR4302534.5774543/1‑94 (MQ=60)
                                                                                      GGATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGTTCGCGTGCTGGACGAAGAAGTCTCCACCGACCAGGCGCGCTGGCATAAC         <  SRR4302534.2533102/94‑1 (MQ=60)
                                                                                       GATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGTTCGCGTGCTGGACGAAGAAGTCTCCACCGACCAGGCGCGCTGGCATAACC        <  SRR4302534.4264822/94‑1 (MQ=60)
                                                                                        ATGGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGTTCGCGTGCTGGACGAAGAAGTCTCCACCGACCAGGCGCGCTGGCATAACCG       >  SRR4302534.2940646/1‑94 (MQ=60)
                                                                                          GGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGTTCGCGTGCTGGACGAAGAAGTCTCCACCGACCAGGCGCGCTGGCATAACCGCT     >  SRR4302534.2634111/1‑94 (MQ=60)
                                                                                          GGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGTTCGCGTGCTGGACGAAGAAGTCTCCACCGACCAGGCGCGCTGGCATAACCGCT     <  SRR4302534.3689512/94‑1 (MQ=60)
                                                                                          GGCGCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGTTCGCGTGCTGGACGAAGAAGTCTCCACCGACCAGGCGCGCTGGCATAACCGCT     >  SRR4302534.831449/1‑94 (MQ=60)
                                                                                             GCCGATACCGTCAAAGTCGGCTGCGATGAATCCCCGGTTCGCATGCTGAACGAAGAAGTCTCCGCGGACCAGTCGCGCTGGCATAACCGGTATT  <  SRR4302534.3446974/94‑1 (MQ=60)
                                                                                             GCCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGTTCGCGTGCTGGACGAAGAAGTCTCCACCGACCAGGCGCGCTGGCATAACCGCTATT  <  SRR4302534.6277370/94‑1 (MQ=60)
                                                                                             |                                                                                             
GTTGATGGCGCAAGCTGGACGCGCACGATGGGCTGGACCGAGTACCAGCAGGTACGCTACGCCACCGCACGCTCAGTCTTCAAATGGGATGGCACCGATACCGTCAAAGTCGGCAGCGATGAAACCCCGGTTCGCGTGCTGGACGAAGAAGTCTCCACCGACCAGGCGCGCTGGCATAACCGCTATT  >  NC_000913/4234195‑4234381

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑