Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	884	257.2	12958910	96.9%	12557183	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,244,613	A→G	Y84C (TAC→TGC)	malK →	ATP‑binding component of transport system for maltose

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,244,613	0	A	G	100.0%	876.2 / NA	249	Y84C (TAC→TGC)	malK	ATP‑binding component of transport system for maltose
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (135/114); total (135/114)

Only 100 of 258 total aligned reads displayed.

CGATCACCAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAGAGGTGATTAACCAACGCG  >  NC_000913/4244523‑4244702

cGATCACCAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGcg                                                                                         >  2:3609181/1‑93 (MQ=255)

  aTCACCAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGcgcg                                                                                       <  2:6255181/93‑1 (MQ=255)

  aTCACCAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGGATGGTGTTTCAGTCTTGcgcg                                                                                       >  2:1480264/1‑93 (MQ=255)

    cACCAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGct                                                                                     <  2:4795948/93‑1 (MQ=255)

     aCCAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGctc                                                                                    >  2:1436308/1‑93 (MQ=255)

     aCCAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGctc                                                                                    >  2:2608945/1‑93 (MQ=255)

     aCCAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGctc                                                                                    >  1:3743023/1‑93 (MQ=255)

      ccAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGctct                                                                                   <  2:3173309/93‑1 (MQ=255)

       cAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTa                                                                                  >  1:2200108/1‑93 (MQ=255)

         gcggcgACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATc                                                                                <  1:1124544/93‑1 (MQ=255)

         gcggcgACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATc                                                                                >  1:5908815/1‑93 (MQ=255)

         gcggcgACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATc                                                                                >  2:2221172/1‑93 (MQ=255)

         gcggcgACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATc                                                                                <  1:3708591/93‑1 (MQ=255)

         gcggcgACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGGATGGTGTTTCAGTCTTGCGCGCtctctc                                                                                >  1:5822470/1‑93 (MQ=255)

          cggcgACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATcc                                                                               <  2:207781/93‑1 (MQ=255)

           ggcgACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGACCGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATccc                                                                              <  2:4517228/93‑1 (MQ=255)

           ggcgACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATccc                                                                              <  1:1590802/93‑1 (MQ=255)

            gcgACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATcccc                                                                             <  1:1465521/93‑1 (MQ=255)

            gcgACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATcccc                                                                             <  1:3856686/93‑1 (MQ=255)

               aCCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCAcc                                                                          <  2:1184168/93‑1 (MQ=255)

               aCCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCAcc                                                                          <  2:6172933/93‑1 (MQ=255)

                ccTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCt                                                                         >  1:643868/1‑93 (MQ=255)

                 cTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTg                                                                        >  1:3922292/1‑93 (MQ=255)

                  tGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGt                                                                       <  2:3474404/93‑1 (MQ=255)

                  tGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGt                                                                       >  2:2365688/1‑93 (MQ=255)

                   gTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTc                                                                      >  2:5832368/1‑93 (MQ=255)

                     tCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAg                                                                    >  2:2389662/1‑93 (MQ=255)

                     tCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAg                                                                    <  1:4496886/93‑1 (MQ=255)

                          ggTGAGACACGGATGAATGACCCTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCa                                                               <  2:2593635/93‑1 (MQ=255)

                          ggTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCa                                                               >  2:6354770/1‑93 (MQ=255)

                            tGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGa                                                             >  2:4906303/1‑93 (MQ=255)

                            tGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGa                                                             >  1:1912686/1‑93 (MQ=255)

                             gagaAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGaa                                                            <  2:5314172/93‑1 (MQ=255)

                                aaaCGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAAc                                                         <  1:5059675/93‑1 (MQ=255)

                                aaaCGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAAc                                                         <  2:5655802/93‑1 (MQ=255)

                                 aaCGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACa                                                        <  2:1736004/93‑1 (MQ=255)

                                 aaCGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACa                                                        >  1:2656716/1‑93 (MQ=255)

                                  aCGGTTGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAGAACAt                                                       >  1:4769191/1‑93 (MQ=255)

                                  aCGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACAt                                                       >  2:3927537/1‑93 (MQ=255)

                                  aCGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACAt                                                       <  1:2706517/93‑1 (MQ=255)

                                  aCGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACAt                                                       <  1:2021610/93‑1 (MQ=255)

                                   cGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATg                                                      >  2:3319829/1‑93 (MQ=255)

                                   cGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATg                                                      <  1:720632/93‑1 (MQ=255)

                                    ggATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGt                                                     >  2:4700134/1‑93 (MQ=255)

                                     gATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTc                                                    >  2:4334295/1‑93 (MQ=255)

                                     gATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTc                                                    >  2:85025/1‑93 (MQ=255)

                                     gATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTc                                                    >  1:3215318/1‑93 (MQ=255)

                                      atgaatgaCACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCa                                                   >  1:5708763/1‑93 (MQ=255)

                                       tgaatgaCACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCAt                                                  >  1:4982536/1‑93 (MQ=255)

                                          atgaCACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTg                                               >  1:4060898/1‑93 (MQ=255)

                                           tgaCACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTgg                                              <  1:6460960/93‑1 (MQ=255)

                                            gaCACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGc                                             <  1:48426/93‑1 (MQ=255)

                                             acacTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGcc                                            <  1:1531172/93‑1 (MQ=255)

                                             acacTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGcc                                            >  2:3824071/1‑93 (MQ=255)

                                             acacTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGcc                                            >  2:2760348/1‑93 (MQ=255)

                                               acTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTg                                          >  1:2365488/1‑93 (MQ=255)

                                                cTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGa                                         <  1:1977532/93‑1 (MQ=255)

                                                 tCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGaa                                        >  2:6096017/1‑93 (MQ=255)

                                                  ccGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGaaa                                       >  1:1413489/1‑93 (MQ=255)

                                                   cGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAAc                                      >  1:2785397/1‑93 (MQ=255)

                                                    gCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCTCCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAAct                                     <  1:1830491/93‑1 (MQ=255)

                                                    gCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAAct                                     <  1:2908695/93‑1 (MQ=255)

                                                     ccAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAActg                                    >  2:1298579/1‑93 (MQ=255)

                                                     ccAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAActg                                    >  2:4928285/1‑93 (MQ=255)

                                                       agcagAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCGCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAActcgc                                  >  1:3107863/1‑93 (MQ=255)

                                                       agcagAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAActggc                                  <  2:5210725/93‑1 (MQ=255)

                                                       agcagAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAActggc                                  <  2:1385893/93‑1 (MQ=255)

                                                        gcagAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAActggct                                 <  1:5996686/93‑1 (MQ=255)

                                                        gcagAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAActggct                                 >  2:2881707/1‑93 (MQ=255)

                                                        gcagAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAActggct                                 <  2:1946761/93‑1 (MQ=255)

                                                          agAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAActggctgg                               <  1:4278225/93‑1 (MQ=255)

                                                           gAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACtggctggc                              <  2:2491714/93‑1 (MQ=255)

                                                           gAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACtggctggc                              >  1:2255136/1‑93 (MQ=255)

                                                           gAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACtggctggc                              >  1:5534967/1‑93 (MQ=255)

                                                             aCGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGgcgc                            >  1:3552745/1‑93 (MQ=255)

                                                                 ggcgTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCaaaa                        >  2:4103429/1‑93 (MQ=255)

                                                                    gTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTTGCGCcaaaaaa                     >  2:5452875/1‑93 (MQ=255)

                                                                    gTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCaaaaaaa                     >  2:6035855/1‑93 (MQ=255)

                                                                       ggTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAagag                  <  2:5132040/93‑1 (MQ=255)

                                                                       ggTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAagag                  >  2:1910637/1‑93 (MQ=255)

                                                                       ggTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTAAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAagag                  >  1:990136/1‑93 (MQ=255)

                                                                        gTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAGAgg                 <  1:267153/93‑1 (MQ=255)

                                                                         tATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAagacgg                >  1:6085459/1‑90 (MQ=255)

                                                                         tATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAGAGGt                <  1:5393741/93‑1 (MQ=255)

                                                                         tATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAGAGGt                >  1:4707619/1‑93 (MQ=255)

                                                                         tATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAGAGGt                <  1:1742123/93‑1 (MQ=255)

                                                                         tATGGTGTTTCAGCCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAGAGGt                <  2:3665178/93‑1 (MQ=255)

                                                                          aTGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAGAGGTg               >  2:3014571/1‑93 (MQ=255)

                                                                           tGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAGAGGTGa              >  2:3093134/1‑93 (MQ=255)

                                                                           tGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAGAGGTGa              <  2:2271329/93‑1 (MQ=255)

                                                                           tGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAGAGGGGa              >  1:4424962/1‑93 (MQ=255)

                                                                             gtgtTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAGAGGTGAtt            <  2:5460374/93‑1 (MQ=255)

                                                                              tgtTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAGAGGTGATTa           >  1:884646/1‑93 (MQ=255)

                                                                                tttCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAGAGGTGATTAAc         >  1:525756/1‑93 (MQ=255)

                                                                                  tCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAGAGGTGATTAACCa       >  2:120900/1‑93 (MQ=255)

                                                                                   cAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAGAGGTGATTAACCaa      >  1:2482441/1‑93 (MQ=255)

                                                                                    aGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAGAGGTGATTAACCAAc     <  2:2017691/93‑1 (MQ=255)

                                                                                    aGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAGAGGTGATTAACCAAc     <  2:3447674/93‑1 (MQ=255)

                                                                                      tCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAGAGGTGATTAACCAAcgc   <  1:3500093/93‑1 (MQ=255)

                                                                                       cTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAGAGGTGATTAACCAAcgcg  <  1:4919860/93‑1 (MQ=255)

CGATCACCAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAGAGGTGATTAACCAACGCG  >  NC_000913/4244523‑4244702

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

AGACGATCACCAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAGAGGTGATTAACCAACGCGTTAA  >  NC_000913/4244520‑4244706

AGACGATCACCAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTG                                                                                               <  SRR4302534.5873310/94‑1 (MQ=60)

AGACGATCACCAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTG                                                                                               >  SRR4302534.4638115/1‑94 (MQ=60)

AGACGATCACCAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTG                                                                                               >  SRR4302534.2311599/1‑94 (MQ=60)

AGACGATCACCAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTG                                                                                               <  SRR4302534.2085702/94‑1 (MQ=60)

 GACGATCACCAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGC                                                                                              >  SRR4302534.2331560/1‑94 (MQ=60)

  ACGATCACCAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCG                                                                                             >  SRR4302534.6246695/1‑94 (MQ=60)

  ACGATCACCAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGATGGTATGGTGTTTCAGTCTTGCG                                                                                             >  SRR4302534.549030/1‑94 (MQ=60)

    GATCACCAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCG                                                                                           >  SRR4302534.927814/1‑94 (MQ=60)

    GATCACCAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCG                                                                                           >  SRR4302534.6652907/1‑94 (MQ=60)

    GATCACCAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCG                                                                                           >  SRR4302534.290243/1‑94 (MQ=60)

    GATCACCAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCG                                                                                           >  SRR4302534.2753023/1‑94 (MQ=60)

      TCACCAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCT                                                                                         >  SRR4302534.4856143/1‑94 (MQ=60)

      TCACCAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCT                                                                                         <  SRR4302534.3133823/94‑1 (MQ=60)

      TCACCAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCT                                                                                         >  SRR4302534.2705066/1‑94 (MQ=60)

       CACCAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTC                                                                                        >  SRR4302534.3843582/1‑94 (MQ=60)

        ACCAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCT                                                                                       <  SRR4302534.6509632/94‑1 (MQ=60)

         CCAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTA                                                                                      >  SRR4302534.2258329/1‑94 (MQ=60)

          CAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTAT                                                                                     >  SRR4302534.397988/1‑94 (MQ=60)

           AGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATC                                                                                    <  SRR4302534.2530294/94‑1 (MQ=60)

           AGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATC                                                                                    >  SRR4302534.6069256/1‑94 (MQ=60)

           AGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATC                                                                                    <  SRR4302534.256059/94‑1 (MQ=60)

           AGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATC                                                                                    >  SRR4302534.4156864/1‑94 (MQ=60)

           AGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGGATGGTGTTTCAGTCTTGCGCGCTCTCTC                                                                                    >  SRR4302534.5980512/1‑94 (MQ=60)

            GCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCC                                                                                   <  SRR4302534.1153937/94‑1 (MQ=60)

            GCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCC                                                                                   <  SRR4302534.3808291/94‑1 (MQ=60)

             CGTCGACCTGTTCATCGATGAGAAGCGGATGAATGGCTCTCCGCTAGCAGAACGCCGCTTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCC                                                                                  <  SRR4302534.1077903/94‑1 (MQ=57)

             CGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCC                                                                                  <  SRR4302534.5075475/94‑1 (MQ=60)

              GGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCC                                                                                 <  SRR4302534.1632408/94‑1 (MQ=60)

               GCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCA                                                                                <  SRR4302534.3960226/94‑1 (MQ=60)

               GCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCA                                                                                <  SRR4302534.1504063/94‑1 (MQ=60)

                 GACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACC                                                                              >  SRR4302534.4960880/1‑94 (MQ=60)

                  ACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCT                                                                             >  SRR4302534.4634768/1‑94 (MQ=60)

                  ACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCT                                                                             >  SRR4302534.659486/1‑94 (MQ=60)

                  ACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCT                                                                             <  SRR4302534.2361461/94‑1 (MQ=60)

                   CCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTG                                                                            >  SRR4302534.4027393/1‑94 (MQ=60)

                    CTGTTCATCGGCGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGT                                                                           >  SRR4302534.2806804/1‑94 (MQ=60)

                        TCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGT                                                                       <  SRR4302534.4618585/94‑1 (MQ=60)

                         CATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTA                                                                      <  SRR4302534.2040774/94‑1 (MQ=60)

                           TCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGC                                                                    >  SRR4302534.3318170/1‑94 (MQ=60)

                             agaGAGAAACAGATGAATGACACGCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAG                                                                  <  SRR4302534.4440333/91‑1 (MQ=60)

                             GGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAG                                                                  <  SRR4302534.440685/94‑1 (MQ=60)

                             GGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAG                                                                  <  SRR4302534.607990/94‑1 (MQ=60)

                              GTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGA                                                                 >  SRR4302534.1962614/1‑94 (MQ=60)

                               TGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAA                                                                >  SRR4302534.4604155/1‑94 (MQ=60)

                                GAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAA                                                               >  SRR4302534.3920237/1‑94 (MQ=60)

                                  GAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAAC                                                             >  SRR4302534.4239303/1‑94 (MQ=60)

                                   AAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACA                                                            <  SRR4302534.5196321/94‑1 (MQ=60)

                                   AAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACA                                                            >  SRR4302534.2726953/1‑94 (MQ=60)

                                   AAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACA                                                            <  SRR4302534.1924259/94‑1 (MQ=60)

                                    AACGGTTGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAGAACAT                                                           >  SRR4302534.4898676/1‑94 (MQ=60)

                                     ACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATG                                                          <  SRR4302534.2074500/94‑1 (MQ=60)

                                     ACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATG                                                          <  SRR4302534.3095365/94‑1 (MQ=60)

                                     ACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATG                                                          <  SRR4302534.1875415/94‑1 (MQ=60)

                                     ACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATG                                                          <  SRR4302534.2777993/94‑1 (MQ=60)

                                      CGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGT                                                         <  SRR4302534.738410/94‑1 (MQ=60)

                                       GGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTC                                                        >  SRR4302534.3300651/1‑94 (MQ=60)

                                        GATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCA                                                       <  SRR4302534.1456550/94‑1 (MQ=60)

                                        GATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCA                                                       >  SRR4302534.5863426/1‑94 (MQ=60)

                                         ATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCAT                                                      >  SRR4302534.5117375/1‑94 (MQ=60)

                                         ATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCAT                                                      <  SRR4302534.71878/94‑1 (MQ=60)

                                          TGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATT                                                     <  SRR4302534.1623761/94‑1 (MQ=60)

                                          TGAAATACACTCCGCCAGCACAACGCGGCCGTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATT                                                     <  SRR4302534.6450786/94‑1 (MQ=60)

                                           GAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTT                                                    <  SRR4302534.5271728/94‑1 (MQ=60)

                                            AATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTG                                                   <  SRR4302534.1982401/94‑1 (MQ=60)

                                            AATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTG                                                   >  SRR4302534.4169825/1‑94 (MQ=60)

                                            AATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTG                                                   >  SRR4302534.364305/1‑94 (MQ=60)

                                             ATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGG                                                  >  SRR4302534.5513072/1‑94 (MQ=60)

                                             ATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGG                                                  <  SRR4302534.5115452/94‑1 (MQ=60)

                                              TGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGC                                                 <  SRR4302534.6634996/94‑1 (MQ=60)

                                              TGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGC                                                 >  SRR4302534.4610213/1‑94 (MQ=60)

                                               GACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCC                                                <  SRR4302534.49721/94‑1 (MQ=60)

                                                ACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCT                                               <  SRR4302534.1571346/94‑1 (MQ=60)

                                                 CACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTG                                              >  SRR4302534.2428293/1‑94 (MQ=60)

                                                 CACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTG                                              >  SRR4302534.6047018/1‑94 (MQ=60)

                                                 CACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTG                                              <  SRR4302534.5602870/94‑1 (MQ=60)

                                                 CACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTG                                              <  SRR4302534.340123/94‑1 (MQ=60)

                                                  ACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGA                                             >  SRR4302534.6629372/1‑94 (MQ=60)

                                                   CTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAA                                            <  SRR4302534.2029312/94‑1 (MQ=60)

                                                   CTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAA                                            <  SRR4302534.3248663/94‑1 (MQ=60)

                                                    TCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAA                                           >  SRR4302534.1450672/1‑94 (MQ=60)

                                                    TCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAA                                           >  SRR4302534.3678348/1‑94 (MQ=60)

                                                     CCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAAC                                          >  SRR4302534.2858657/1‑94 (MQ=60)

                                                      CGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACT                                         >  SRR4302534.1490279/1‑94 (MQ=60)

                                                      CGCCAGCAGAACGCAGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACT                                         >  SRR4302534.6366474/1‑94 (MQ=60)

                                                       GCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCTCCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTG                                        <  SRR4302534.1878100/94‑1 (MQ=60)

                                                       GCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTG                                        <  SRR4302534.4951710/94‑1 (MQ=60)

                                                       GCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTG                                        <  SRR4302534.2985043/94‑1 (MQ=60)

                                                        CCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGG                                       >  SRR4302534.2733745/1‑94 (MQ=60)

                                                         CAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCGCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTCGC                                      >  SRR4302534.3189589/1‑94 (MQ=60)

                                                         CAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGC                                      >  SRR4302534.2081285/1‑94 (MQ=60)

                                                          AGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCT                                     <  SRR4302534.1580250/94‑1 (MQ=60)

                                                          AGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCT                                     >  SRR4302534.3688786/1‑94 (MQ=60)

                                                           GCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTG                                    <  SRR4302534.6159598/94‑1 (MQ=60)

                                                           GCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTG                                    <  SRR4302534.911743/94‑1 (MQ=60)

                                                           GCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTG                                    >  SRR4302534.5759045/1‑94 (MQ=60)

                                                            CAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGG                                   >  SRR4302534.3816499/1‑94 (MQ=60)

                                                             AGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGC                                  >  SRR4302534.2314912/1‑94 (MQ=60)

                                                             AGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGC                                  <  SRR4302534.1530450/94‑1 (MQ=60)

                                                             AGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGC                                  <  SRR4302534.4393988/94‑1 (MQ=60)

                                                             AGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGC                                  >  SRR4302534.5684375/1‑94 (MQ=60)

                                                             AGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGC                                  >  SRR4302534.6179668/1‑94 (MQ=60)

                                                              GAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCG                                 >  SRR4302534.5118517/1‑94 (MQ=60)

                                                               AACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGC                                >  SRR4302534.3648289/1‑94 (MQ=60)

                                                                ACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCA                               >  SRR4302534.6029221/1‑94 (MQ=60)

                                                                ACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCA                               >  SRR4302534.896798/1‑94 (MQ=60)

                                                                     GCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAA                          <  SRR4302534.2436598/94‑1 (MQ=60)

                                                                      CGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAA                         >  SRR4302534.4884050/1‑94 (MQ=60)

                                                                        TTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAGA                       >  SRR4302534.1839895/1‑94 (MQ=60)

                                                                         TGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAGAG                      <  SRR4302534.5927219/94‑1 (MQ=60)

                                                                         TGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTAAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAGAG                      >  SRR4302534.1015474/1‑94 (MQ=60)

                                                                          GGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAGAGG                     <  SRR4302534.2761059/94‑1 (MQ=60)

                                                                           GTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAGAcgg                    >  SRR4302534.6250934/1‑91 (MQ=60)

                                                                           GTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAGAGGT                    <  SRR4302534.274081/94‑1 (MQ=60)

                                                                           GTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAGAGGT                    >  SRR4302534.4835420/1‑94 (MQ=60)

                                                                           GTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAGAGGG                    >  SRR4302534.2294613/1‑94 (MQ=60)

                                                                            TATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAGAGGTG                   >  SRR4302534.2283766/1‑94 (MQ=60)

                                                                            TATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAGAGGTG                   <  SRR4302534.5538969/94‑1 (MQ=60)

                                                                            TATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAGAGGTG                   <  SRR4302534.1444219/94‑1 (MQ=60)

                                                                            TATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAGAGGTG                   <  SRR4302534.2039668/94‑1 (MQ=60)

                                                                            TATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAGAGGTG                   <  SRR4302534.1787348/94‑1 (MQ=60)

                                                                             ATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAGAGGTGA                  >  SRR4302534.1744003/1‑94 (MQ=60)

                                                                             ATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAGAGGGGA                  >  SRR4302534.4544434/1‑94 (MQ=60)

                                                                              TGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAGAGGGGAT                 >  SRR4302534.537211/1‑94 (MQ=60)

                                                                                GTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAGAGGTGATTA               >  SRR4302534.907033/1‑94 (MQ=60)

                                                                                GTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAGAGGTGATTA               <  SRR4302534.1939969/94‑1 (MQ=60)

                                                                                 TGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAGAGGTGATTAA              <  SRR4302534.3998642/94‑1 (MQ=60)

                                                                                 TGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAGAGGTGATTAA              >  SRR4302534.4239639/1‑94 (MQ=60)

                                                                                  GTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAGAGGTGATTAAC             >  SRR4302534.538817/1‑94 (MQ=60)

                                                                                     TCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAGAGGTGATTAACCAA          >  SRR4302534.2548368/1‑94 (MQ=60)

                                                                                     TCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAGAGGCGAATAACCAA          >  SRR4302534.3943471/1‑94 (MQ=60)

                                                                                      CAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAGAGGTGATTAACCAAC         <  SRR4302534.5051957/94‑1 (MQ=60)

                                                                                         TCTTGCGCGCTCTATCCCCCCCTGTCAGTAGCAGAAAGCATGTCATTGGGCCTGAAACTGGCAGGCGCAAAAAAAGAGGTGATTAACCAACGCG      <  SRR4302534.2912999/94‑1 (MQ=60)

                                                                                         TCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAGAGGTGATTAACCAACGCG      <  SRR4302534.3594214/94‑1 (MQ=60)

                                                                                          CTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAGAGGTGATTAACCAACGCGT     <  SRR4302534.5053200/94‑1 (MQ=60)

                                                                                           TTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAGAGGTGATTAACCAACGCGTT    <  SRR4302534.3516602/94‑1 (MQ=60)

                                                                                           TTGCGCGCTCTATCCACTCCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAGAGGTGATTAACCAACGCGTT    <  SRR4302534.6385551/94‑1 (MQ=60)

                                                                                            TGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAGAGGTGATTAACCAACGCGTTA   >  SRR4302534.3954355/1‑94 (MQ=60)

                                                                                            TGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAGAGGTGATTAACCAACGCGTTA   <  SRR4302534.2013470/94‑1 (MQ=60)

                                                                                            TGCGCCCTCTATCCCGACCTGTCAGTCGCAGAAACCATGTCATTTGGCATGAAACTGGCTGGCGCAAAAAAAGAGGTGATCAACCAACGCGTTA   <  SRR4302534.2443365/94‑1 (MQ=60)

                                                                                             GCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAGAGGTGATTAACCAACGCGTTAA  >  SRR4302534.25301/1‑94 (MQ=60)

AGACGATCACCAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAGAGGTGATTAACCAACGCGTTAA  >  NC_000913/4244520‑4244706

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`