Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 884 257.2 12958910 96.9% 12557183 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,253,868 A→G C60R (TGT→CGT)  plsB ← glycerol‑3‑phosphate acyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,253,8680AG100.0% 862.3 / NA 247C60R (TGT→CGT) plsBglycerol‑3‑phosphate acyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (114/133);  total (114/133)

Only 100 of 252 total aligned reads displayed.

GCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGG  >  NC_000913/4253778‑4253957
                                                                                          |                                                                                         
gcgcCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCt                                                                                         <  1:6188431/93‑1 (MQ=255)
 cgccccGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTg                                                                                        <  1:1419828/90‑1 (MQ=255)
 cgGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTg                                                                                        <  1:1508141/93‑1 (MQ=255)
 cgGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTg                                                                                        >  1:2606480/1‑93 (MQ=255)
   gCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTggg                                                                                      >  2:379699/1‑93 (MQ=255)
   ccccGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTggg                                                                                      <  1:6353973/92‑1 (MQ=255)
   ccccGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTggg                                                                                      <  1:2304811/92‑1 (MQ=255)
    cccGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGgc                                                                                     <  1:1690143/93‑1 (MQ=255)
    cccGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGgc                                                                                     <  2:5210601/93‑1 (MQ=255)
      cgccgTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGAGTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGgcgc                                                                                   >  2:4525428/1‑93 (MQ=255)
       gccgTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGgcgcg                                                                                  >  1:5622531/1‑93 (MQ=255)
        ccgTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGgcgcgc                                                                                 >  1:4906386/1‑93 (MQ=255)
        ccgTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGgcgcgc                                                                                 <  2:2323097/93‑1 (MQ=255)
          gTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCaa                                                                               >  2:2885515/1‑93 (MQ=255)
             aatgaaCACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGt                                                                            <  2:5927810/93‑1 (MQ=255)
                 aaCACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGc                                                                        <  2:225902/93‑1 (MQ=255)
                 aaCACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGc                                                                        >  2:50706/1‑93 (MQ=255)
                  acacATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCa                                                                       >  2:4684223/1‑93 (MQ=255)
                   cacaTAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCaa                                                                      >  1:698889/1‑93 (MQ=255)
                      aTAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATc                                                                   >  1:4395434/1‑93 (MQ=255)
                      aTAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATc                                                                   <  2:10315/93‑1 (MQ=255)
                       tAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCt                                                                  >  2:1570632/1‑93 (MQ=255)
                       tAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCt                                                                  <  2:698889/93‑1 (MQ=255)
                        aGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTg                                                                 >  1:3401803/1‑93 (MQ=255)
                         gcgcGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGc                                                                >  2:6030236/1‑93 (MQ=255)
                         gcgcGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGc                                                                >  1:2958716/1‑93 (MQ=255)
                         gcgcGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGc                                                                <  2:6339475/93‑1 (MQ=255)
                          cgcgGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCt                                                               <  2:5962203/93‑1 (MQ=255)
                            cgGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCttt                                                             <  2:3665443/93‑1 (MQ=255)
                              gCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCg                                                           >  1:5758005/1‑93 (MQ=255)
                              gCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCg                                                           <  1:1930506/93‑1 (MQ=255)
                              gCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCg                                                           >  2:5893498/1‑93 (MQ=255)
                               cAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGa                                                          >  1:6041777/1‑93 (MQ=255)
                                aGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAg                                                         <  2:2666238/93‑1 (MQ=255)
                                 gTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGt                                                        >  2:4471356/1‑93 (MQ=255)
                                 gTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGt                                                        <  2:5751977/93‑1 (MQ=255)
                                 gTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGt                                                        <  1:1568206/93‑1 (MQ=255)
                                 gTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGt                                                        >  2:2512907/1‑93 (MQ=255)
                                  tAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGtt                                                       >  2:4809661/1‑93 (MQ=255)
                                  tAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGtt                                                       <  1:4979280/93‑1 (MQ=255)
                                   aGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTg                                                      <  1:2691213/93‑1 (MQ=255)
                                   aGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGGCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTg                                                      >  2:3446141/1‑93 (MQ=255)
                                    gCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGt                                                     >  2:1435817/1‑93 (MQ=255)
                                     cGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTCCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTa                                                    <  1:5184993/93‑1 (MQ=255)
                                      gtcccGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTAc                                                   <  2:3334323/90‑1 (MQ=255)
                                      gTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTAc                                                   >  2:4264793/1‑93 (MQ=255)
                                      gTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTAc                                                   >  2:549258/1‑93 (MQ=255)
                                       tGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACg                                                  <  1:5299845/93‑1 (MQ=255)
                                         ccGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGt                                                <  2:4168034/93‑1 (MQ=255)
                                         ccGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGt                                                >  2:3590464/1‑93 (MQ=255)
                                         ccGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGt                                                <  2:1272823/93‑1 (MQ=255)
                                           gTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTaa                                              >  2:1067300/1‑93 (MQ=255)
                                           gTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTaa                                              <  1:82611/93‑1 (MQ=255)
                                           gTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTaa                                              >  1:2063843/1‑93 (MQ=255)
                                              gATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAAc                                           >  1:5970608/1‑93 (MQ=255)
                                               aTTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACg                                          <  1:5198969/93‑1 (MQ=255)
                                                   ccAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACa                                      <  1:4713765/93‑1 (MQ=255)
                                                    cAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACAt                                     <  1:5466208/93‑1 (MQ=255)
                                                    cAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACAt                                     <  1:1343289/93‑1 (MQ=255)
                                                      gCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATaa                                   >  1:3600162/1‑93 (MQ=255)
                                                         gCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTg                                <  1:4527160/93‑1 (MQ=255)
                                                          gtctAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTgg                               <  2:1957241/92‑1 (MQ=255)
                                                          ctctAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTgg                               <  2:205433/93‑1 (MQ=255)
                                                           tctAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGa                              >  1:1765670/1‑93 (MQ=255)
                                                           tctAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGa                              >  2:3970360/1‑93 (MQ=255)
                                                           tctAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGATGTACGGTAAAACGTACATAATTggc                              >  1:379852/1‑92 (MQ=255)
                                                           tctAACGGGTCAGGCAAGTAATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGa                              <  1:4758968/93‑1 (MQ=255)
                                                            ctAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGAc                             <  2:371111/93‑1 (MQ=255)
                                                             tAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACg                            >  1:5894884/1‑93 (MQ=255)
                                                             tAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACg                            >  2:3891497/1‑93 (MQ=255)
                                                              aaCGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACga                           >  1:5800607/1‑93 (MQ=255)
                                                              aaCGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACga                           <  1:3217298/93‑1 (MQ=255)
                                                               aCGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACgag                          >  2:79305/1‑93 (MQ=255)
                                                               aCGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGGACATAATTGGACgag                          >  2:6143569/1‑93 (MQ=255)
                                                                cGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACgaga                         <  1:2822955/93‑1 (MQ=255)
                                                                cGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACgaga                         <  1:6435330/93‑1 (MQ=255)
                                                                 gggTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGagag                        >  1:1009208/1‑93 (MQ=255)
                                                                 gggTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGagag                        <  2:4308475/93‑1 (MQ=255)
                                                                  ggTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAgg                       <  2:5522074/93‑1 (MQ=255)
                                                                   gTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGt                      <  1:3097220/93‑1 (MQ=255)
                                                                    tCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTa                     <  2:3568755/93‑1 (MQ=255)
                                                                     cAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTAt                    >  2:6207663/1‑93 (MQ=255)
                                                                       ggCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATcc                  >  1:1625966/1‑93 (MQ=255)
                                                                       ggCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATcc                  >  1:5297548/1‑93 (MQ=255)
                                                                       ggCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATcc                  >  2:439771/1‑93 (MQ=255)
                                                                        gCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCa                 <  2:3364735/93‑1 (MQ=255)
                                                                          aaGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGc               >  2:1797239/1‑93 (MQ=255)
                                                                          aaGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGc               >  1:4038945/1‑93 (MQ=255)
                                                                           aGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGcc              >  2:5727377/1‑93 (MQ=255)
                                                                            gTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGccc             >  1:1989179/1‑93 (MQ=255)
                                                                              cATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCa           >  2:3269036/1‑93 (MQ=255)
                                                                               aTGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAg          >  2:1861712/1‑93 (MQ=255)
                                                                                tgtgCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGt         <  2:4006962/93‑1 (MQ=255)
                                                                                tgtgCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGt         <  1:3943667/93‑1 (MQ=255)
                                                                                 gtgCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGtt        <  1:1073424/93‑1 (MQ=255)
                                                                                    ccAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCg     >  2:5289814/1‑93 (MQ=255)
                                                                                      aGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCggg   <  1:289286/93‑1 (MQ=255)
                                                                                       gACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCgggg  >  2:3523595/1‑93 (MQ=255)
                                                                                       gACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCgggg  <  2:5026976/93‑1 (MQ=255)
                                                                                       gACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCgggg  >  2:4735527/1‑93 (MQ=255)
                                                                                          |                                                                                         
GCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGG  >  NC_000913/4253778‑4253957

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAG  >  NC_000913/4253776‑4253960
                                                                                            |                                                                                            
ACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGC                                                                                             >  SRR4302534.3834114/1‑94 (MQ=60)
 CGCGCCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCT                                                                                            <  SRR4302534.2047678/94‑1 (MQ=60)
  GCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTG                                                                                           >  SRR4302534.2675443/1‑94 (MQ=60)
  GCGCCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTG                                                                                           <  SRR4302534.5013031/94‑1 (MQ=60)
  GCGCCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTG                                                                                           <  SRR4302534.6356849/94‑1 (MQ=60)
  GCGCCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTG                                                                                           <  SRR4302534.4471471/94‑1 (MQ=60)
   CGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGG                                                                                          <  SRR4302534.1547804/94‑1 (MQ=60)
   CGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTACCAGACGCTGG                                                                                          >  SRR4302534.5075733/1‑94 (MQ=60)
   CGCCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGG                                                                                          <  SRR4302534.3199716/94‑1 (MQ=60)
   CGCCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGG                                                                                          <  SRR4302534.1457188/94‑1 (MQ=60)
    GGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGATGCTGGG                                                                                         >  SRR4302534.1680589/1‑94 (MQ=60)
     CCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGC                                                                                        <  SRR4302534.6525577/94‑1 (MQ=60)
     CCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGC                                                                                        <  SRR4302534.2365982/94‑1 (MQ=60)
      CCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCG                                                                                       <  SRR4302534.1734077/94‑1 (MQ=60)
        CGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCG                                                                                     >  SRR4302534.5774676/1‑94 (MQ=60)
         GCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGC                                                                                    >  SRR4302534.5039382/1‑94 (MQ=60)
          CCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCA                                                                                   >  SRR4302534.120737/1‑94 (MQ=60)
           CGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAA                                                                                  >  SRR4302534.6136912/1‑94 (MQ=60)
               AATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTC                                                                              <  SRR4302534.6425554/94‑1 (MQ=60)
                 TGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAG                                                                            >  SRR4302534.6003021/1‑94 (MQ=60)
                 TGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAG                                                                            <  SRR4302534.3819239/94‑1 (MQ=60)
                    ACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAA                                                                         <  SRR4302534.3067518/94‑1 (MQ=60)
                    ACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAA                                                                         >  SRR4302534.715996/1‑94 (MQ=60)
                     CACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAA                                                                        >  SRR4302534.1966410/1‑94 (MQ=60)
                      ACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAAT                                                                       <  SRR4302534.6635692/94‑1 (MQ=60)
                      ACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAAT                                                                       >  SRR4302534.279268/1‑94 (MQ=60)
                       CATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATC                                                                      >  SRR4302534.4513992/1‑94 (MQ=60)
                       CATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATC                                                                      >  SRR4302534.3916662/1‑94 (MQ=60)
                        ATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCT                                                                     <  SRR4302534.541017/94‑1 (MQ=60)
                        ATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCT                                                                     <  SRR4302534.4595402/94‑1 (MQ=60)
                         TAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTG                                                                    >  SRR4302534.3493138/1‑94 (MQ=60)
                         TAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTG                                                                    >  SRR4302534.3383614/1‑94 (MQ=60)
                         TAGCGCGGCAGTAGCGTCCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTG                                                                    <  SRR4302534.514615/94‑1 (MQ=60)
                          AGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGC                                                                   >  SRR4302534.3036252/1‑94 (MQ=60)
                          AGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAAAGTCAGCAAATCTGC                                                                   >  SRR4302534.5897967/1‑94 (MQ=60)
                           GCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCT                                                                  <  SRR4302534.2022106/94‑1 (MQ=60)
                            CGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTT                                                                 >  SRR4302534.1581106/1‑94 (MQ=60)
                              CGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTC                                                               >  SRR4302534.5930131/1‑94 (MQ=60)
                               GGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCG                                                              >  SRR4302534.5914192/1‑94 (MQ=60)
                               GGCAGTAGCGTGCCGTCGATTTACAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCG                                                              <  SRR4302534.4330140/94‑1 (MQ=60)
                                GCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGA                                                             <  SRR4302534.1980980/94‑1 (MQ=60)
                                GCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGA                                                             <  SRR4302534.3395039/94‑1 (MQ=60)
                                GCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGA                                                             >  SRR4302534.6205999/1‑94 (MQ=60)
                                 CAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAG                                                            <  SRR4302534.1450925/94‑1 (MQ=60)
                                 CAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAG                                                            >  SRR4302534.173839/1‑94 (MQ=60)
                                   GTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTT                                                          <  SRR4302534.2907268/94‑1 (MQ=60)
                                   GTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTT                                                          <  SRR4302534.6116991/94‑1 (MQ=60)
                                   GTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTT                                                          <  SRR4302534.1609280/94‑1 (MQ=60)
                                   GTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTT                                                          <  SRR4302534.669205/94‑1 (MQ=60)
                                   ATAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCGGCTTTCGAGTT                                                          <  SRR4302534.482457/94‑1 (MQ=60)
                                    tggcaTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTG                                                         <  SRR4302534.364280/89‑1 (MQ=60)
                                    TAGCGTGCCGTCGGTTTCCAGCGGCTCTAACGGGTCAGGCAAGTCAGGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTG                                                         <  SRR4302534.5505001/94‑1 (MQ=60)
                                    TAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTG                                                         <  SRR4302534.5114054/94‑1 (MQ=60)
                                     AGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGT                                                        >  SRR4302534.721137/1‑94 (MQ=60)
                                     AGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGT                                                        <  SRR4302534.2762328/94‑1 (MQ=60)
                                      GCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTA                                                       <  SRR4302534.3532801/94‑1 (MQ=60)
                                       CGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTCCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTAC                                                      <  SRR4302534.5324750/94‑1 (MQ=60)
                                        GTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACG                                                     >  SRR4302534.300796/1‑94 (MQ=60)
                                        GTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACG                                                     <  SRR4302534.1637917/94‑1 (MQ=60)
                                        GTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACG                                                     <  SRR4302534.2735069/94‑1 (MQ=60)
                                         TGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGG                                                    <  SRR4302534.5442861/94‑1 (MQ=60)
                                          GCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGT                                                   <  SRR4302534.2040847/94‑1 (MQ=60)
                                           CCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTA                                                  <  SRR4302534.5325433/94‑1 (MQ=60)
                                            CGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAA                                                 >  SRR4302534.2117807/1‑94 (MQ=60)
                                            CGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAA                                                 >  SRR4302534.209895/1‑94 (MQ=60)
                                            CGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTAAGGTAA                                                 >  SRR4302534.290649/1‑94 (MQ=60)
                                             GTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAA                                                <  SRR4302534.84827/94‑1 (MQ=60)
                                               CGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAAC                                              >  SRR4302534.6132770/1‑94 (MQ=60)
                                               CGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAAC                                              <  SRR4302534.1353103/94‑1 (MQ=60)
                                               CGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAAC                                              >  SRR4302534.5770754/1‑94 (MQ=60)
                                                 ATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGT                                            <  SRR4302534.5339209/94‑1 (MQ=60)
                                                    TCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACA                                         <  SRR4302534.5824117/94‑1 (MQ=60)
                                                    TCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACA                                         <  SRR4302534.1470909/94‑1 (MQ=60)
                                                     CCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACAT                                        <  SRR4302534.4841777/94‑1 (MQ=60)
                                                     CCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACAT                                        <  SRR4302534.1018613/94‑1 (MQ=60)
                                                     CCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACAT                                        <  SRR4302534.3847021/94‑1 (MQ=60)
                                                      CAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATA                                       <  SRR4302534.1378709/94‑1 (MQ=60)
                                                      CAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATA                                       <  SRR4302534.5613549/94‑1 (MQ=60)
                                                       AGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAA                                      >  SRR4302534.4095830/1‑94 (MQ=60)
                                                       AGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAA                                      >  SRR4302534.3696981/1‑94 (MQ=60)
                                                         CGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATT                                    >  SRR4302534.1043933/1‑94 (MQ=60)
                                                         CGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATT                                    <  SRR4302534.6597443/94‑1 (MQ=60)
                                                         CGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATT                                    <  SRR4302534.2723380/94‑1 (MQ=60)
                                                         CGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATT                                    >  SRR4302534.4685229/1‑94 (MQ=60)
                                                           GCTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGG                                  <  SRR4302534.4649803/94‑1 (MQ=60)
                                                            CTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGA                                 >  SRR4302534.1811519/1‑94 (MQ=60)
                                                            CTCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGATGTACGGTAAAACGTACATAATTGGC                                 >  SRR4302534.389564/1‑94 (MQ=60)
                                                             TCTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGAC                                <  SRR4302534.4861683/94‑1 (MQ=60)
                                                             TCTAACGGGTCAGGCAAGTAATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGAC                                <  SRR4302534.4888193/94‑1 (MQ=60)
                                                              CTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACG                               >  SRR4302534.4663844/1‑94 (MQ=60)
                                                              CTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACG                               >  SRR4302534.6396387/1‑94 (MQ=60)
                                                              CTAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACG                               >  SRR4302534.6054935/1‑94 (MQ=60)
                                                               TAACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGA                              >  SRR4302534.5958028/1‑94 (MQ=60)
                                                                AACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAG                             <  SRR4302534.3302688/94‑1 (MQ=60)
                                                                AACGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAG                             >  SRR4302534.223476/1‑94 (MQ=60)
                                                                  CGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAG                           >  SRR4302534.1035133/1‑94 (MQ=60)
                                                                  CGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAG                           <  SRR4302534.2897173/94‑1 (MQ=60)
                                                                  CGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAG                           <  SRR4302534.6608795/94‑1 (MQ=60)
                                                                  CGGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAG                           <  SRR4302534.1641933/94‑1 (MQ=60)
                                                                   GGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGG                          >  SRR4302534.5158076/1‑94 (MQ=60)
                                                                   GGGTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGG                          >  SRR4302534.1802536/1‑94 (MQ=60)
                                                                     GTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTA                        <  SRR4302534.3178689/94‑1 (MQ=60)
                                                                     GTCAGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTA                        <  SRR4302534.3116686/94‑1 (MQ=60)
                                                                        AGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCC                     >  SRR4302534.5440526/1‑94 (MQ=60)
                                                                        AGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCC                     >  SRR4302534.6136114/1‑94 (MQ=60)
                                                                        AGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCC                     >  SRR4302534.1668391/1‑94 (MQ=60)
                                                                        AGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCC                     >  SRR4302534.6267525/1‑94 (MQ=60)
                                                                        AGGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCC                     <  SRR4302534.4935186/94‑1 (MQ=60)
                                                                         GGCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCA                    <  SRR4302534.1472176/94‑1 (MQ=60)
                                                                          GCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAG                   <  SRR4302534.100690/94‑1 (MQ=60)
                                                                          GCAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAG                   >  SRR4302534.5441579/1‑94 (MQ=60)
                                                                           CAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGC                  >  SRR4302534.2162733/1‑94 (MQ=60)
                                                                           CAAGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGC                  >  SRR4302534.4147239/1‑94 (MQ=60)
                                                                             AGTCATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCC                >  SRR4302534.2041268/1‑94 (MQ=60)
                                                                                CATGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAG             >  SRR4302534.543953/1‑94 (MQ=60)
                                                                                  TGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTT           <  SRR4302534.4049269/94‑1 (MQ=60)
                                                                                  TGTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTT           <  SRR4302534.4049270/94‑1 (MQ=60)
                                                                                   GTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTC          >  SRR4302534.5435840/1‑94 (MQ=60)
                                                                                   GTGCCAGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTC          <  SRR4302534.1101279/94‑1 (MQ=60)
                                                                                        AGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGG     >  SRR4302534.5998576/1‑94 (MQ=60)
                                                                                        AGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGG     >  SRR4302534.2613267/1‑94 (MQ=60)
                                                                                        AGACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGG     <  SRR4302534.296769/94‑1 (MQ=60)
                                                                                          ACGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCA   <  SRR4302534.3332815/94‑1 (MQ=60)
                                                                                           CGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAG  <  SRR4302534.646396/94‑1 (MQ=60)
                                                                                           CGCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAG  >  SRR4302534.1319648/1‑94 (MQ=60)
                                                                                            |                                                                                            
ACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAG  >  NC_000913/4253776‑4253960

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑