Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 884 257.2 12958910 96.9% 12557183 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,296,466 A→G A160A (GCT→GCC)  fdhF ← selenopolypeptide subunit of formate dehydrogenase H

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,296,4660AG100.0% 893.3 / NA 254A160A (GCT→GCC) fdhFselenopolypeptide subunit of formate dehydrogenase H
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (141/113);  total (141/113)

Only 100 of 262 total aligned reads displayed.

GTTAATTACGTGATTCGCCACGATTGGGTGGGAATCCGCCGGGTTGTACCCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATAGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGTGTCAGACACGAGCGCAGCAGTCAACGTTATT  >  NC_000913/4296376‑4296555
                                                                                          |                                                                                         
gTTAATTACGTGATTCGCCACGATTGGGTGGGAATCCGCCGGGTTGTACCCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGc                                                                                         <  2:472756/93‑1 (MQ=255)
gTTAATTACGTGATTCGCCACGATTGGGTGGGAATCCGCCGGGTTGTACCCGAAAACGAACACTAAATAGGTATTATCAATTTCGTTAATGGc                                                                                         >  2:2066554/1‑93 (MQ=255)
  tAATTACGTGATTCGCCACGATTGGGTGGGAATCCGCCGGGTTGTACCCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGc                                                                                         <  2:1817752/91‑1 (MQ=255)
    aTTACGTGATTCGCCACGATTGGGTGGGAATCCGCCGGGTTGTACCCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTg                                                                                     >  2:6309035/1‑93 (MQ=255)
    aTTACGTGATTCGCCACGATTGGGTGGGAATCCGCCGGGTTGTACCCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTg                                                                                     >  2:1081295/1‑93 (MQ=255)
    aTTACGTGATTCGCCACGATTGGGTGGGAATCCGCCGGGTTGTACCCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTg                                                                                     >  2:2462362/1‑93 (MQ=255)
     ttACGTGATTCGCCACGATTGGGTGGGAATCCGCCGGGTTGTACCCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGc                                                                                    <  1:2964668/93‑1 (MQ=255)
     ttACGTGATTCGCCACGATTGGGTGGGAATCCGCCGGGCTGTACCCGAAAACGAACACTAAATCGGAATTATCAATTTCGTTAATGGCATTGc                                                                                    >  2:3109727/1‑93 (MQ=255)
      tACGTGATTCGCCACGATTGGGTGGGAATCCGCCGGGTTGTGCCCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCt                                                                                   <  2:2118682/93‑1 (MQ=255)
      tACGTGATTCGCCACGATTGGGTGGGAATCCGCCGGGTTGTACCCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCt                                                                                   >  2:4166948/1‑93 (MQ=255)
       aCGTGATTCGCCACGATTGGGTGGGAATCCGCCGGGTTGTACCCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTc                                                                                  <  2:2964062/93‑1 (MQ=255)
          tGATTCGCCACGATTGGGTGGGAATCCGCCGGGTTGTACCCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCAtt                                                                               <  1:4836795/93‑1 (MQ=255)
           gATTCGCCACGATTGGGTGGGAATCCGCCGGGTTGTACCCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTg                                                                              <  2:522302/93‑1 (MQ=255)
           gATTCGCCACGATTGGGTGGGAATCCGCCGGGTTGTACCCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTg                                                                              >  2:5399400/1‑93 (MQ=255)
            aTTCGCCACGATTGGGTGGGAATCCGCCGGGTTGTACCCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTgc                                                                             >  2:167457/1‑93 (MQ=255)
               cGCCACGATTGGGTGGGAATCCGCCGGGTTGTACCCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGcc                                                                          <  1:4991176/93‑1 (MQ=255)
                 ccACGATTGGGTGGGAATCCGCCGGGTTGTACCCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCAt                                                                        >  2:1914947/1‑93 (MQ=255)
                 ccACGATTGGGTGGGAATCCGCCGGGTTGTACCCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCAt                                                                        >  2:5111528/1‑93 (MQ=255)
                 ccACGATTGGGTGGGAATCCGCCGGGTTGTACCCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCAt                                                                        >  2:1584079/1‑93 (MQ=255)
                 ccACGATTGGGTGGGAATCCGCCGGGTTGTACCCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCAt                                                                        <  2:6366256/93‑1 (MQ=255)
                  cACGATTGGGTGGGAATCCGCCGGGTTGTACCCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCAtt                                                                       <  1:4849439/93‑1 (MQ=255)
                    cGATTGGGTGGGAATCCGCCGGGTTGTACCCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTac                                                                     <  1:6455090/93‑1 (MQ=255)
                    cGATTGGGTGGGAATCCGCCGGGTTGTACCCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTac                                                                     <  2:4983964/93‑1 (MQ=255)
                      aTTGGGTGGGAATCCGCCGGGTTGTACCCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTaccg                                                                   >  2:4889557/1‑93 (MQ=255)
                      aTTGGGTGGGAATCCGCCGGGTTGTACCCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTaccg                                                                   >  1:3489148/1‑93 (MQ=255)
                       ttGGGTGGGAATCCGCCGGGTTGTACCCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTaccga                                                                  <  1:4509883/93‑1 (MQ=255)
                        tgggtgggAATCCGCCGGGTTGTACCCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTaccgac                                                                 <  1:3878857/93‑1 (MQ=255)
                         gggtgggAATCCGCCGGGTTGTACCCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTaccgacc                                                                <  1:2068685/93‑1 (MQ=255)
                          ggggggAATCCGCCGGGTTGTACCCGAAAACGAACACTAAATCGGAATTATCAATTTAGTTAATGGCATTGCTCATTGCGCCATTaccgaccg                                                               >  1:4344706/4‑93 (MQ=255)
                            tgggAATCCGCCGGGTTGTACCCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGAt                                                             >  2:2858795/1‑93 (MQ=255)
                                 aTCCGCCGGGTTGTACCCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGGATTGCTCATTGCGCCATTACCGACCGATTGGTg                                                        >  1:1225677/1‑93 (MQ=255)
                                 aTCCGCCGGGTTGTACCCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTg                                                        <  1:2455882/93‑1 (MQ=255)
                                  tCCGCCGGGTTGTACCCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGc                                                       <  2:2657291/93‑1 (MQ=255)
                                  tCCGCCGGGTTGTACCCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGc                                                       <  1:5556142/93‑1 (MQ=255)
                                      ccgGGTTGTACCCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGAc                                                   >  2:3802008/1‑93 (MQ=255)
                                        gggTTGTACCCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCt                                                 >  1:1827173/1‑93 (MQ=255)
                                        gggTTGTACCCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCt                                                 >  2:2302585/1‑93 (MQ=255)
                                         ggTTGTACCCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTg                                                >  2:4568501/1‑93 (MQ=255)
                                           ttGTACCCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCa                                              >  2:3342916/1‑93 (MQ=255)
                                             gTACCCGAAATCGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAAc                                            <  1:3193157/93‑1 (MQ=255)
                                              tACCCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAAcc                                           >  1:3343897/1‑93 (MQ=255)
                                              tACCCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAAcc                                           >  1:46654/1‑93 (MQ=255)
                                                cccGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGa                                         <  1:6024009/93‑1 (MQ=255)
                                                 ccGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGAt                                        <  1:4214153/93‑1 (MQ=255)
                                                  cGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATg                                       <  1:5088225/93‑1 (MQ=255)
                                                  cGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATg                                       <  1:1043960/93‑1 (MQ=255)
                                                   gAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATgg                                      >  1:822024/1‑93 (MQ=255)
                                                    aaaaCGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATggg                                     >  2:4484521/1‑93 (MQ=255)
                                                    aaaaCGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATggg                                     >  1:2823125/1‑93 (MQ=255)
                                                     aaaCGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGc                                    <  1:5484593/93‑1 (MQ=255)
                                                     aaaCGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGc                                    >  2:3738771/1‑93 (MQ=255)
                                                     aaaCGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGc                                    >  2:5767359/1‑93 (MQ=255)
                                                       aCGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCg                                  >  2:87824/1‑93 (MQ=255)
                                                       aCGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCg                                  <  1:887430/93‑1 (MQ=255)
                                                        cGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCgt                                 >  1:2298241/1‑93 (MQ=255)
                                                          aaCACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCgtgt                               >  2:1242649/1‑93 (MQ=255)
                                                            cacTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGTGTCa                             >  2:5969247/1‑93 (MQ=255)
                                                             acTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGTGTCAg                            >  2:5579306/1‑93 (MQ=255)
                                                             acTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGTGTCAg                            <  2:5884694/93‑1 (MQ=255)
                                                             acTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGTGTCAg                            >  1:4133804/1‑93 (MQ=255)
                                                               tAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGTGTCAGac                          >  1:2396871/1‑93 (MQ=255)
                                                                aaaTCGGGATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGGTGGGCCGTGTCAGaca                         >  1:2143555/1‑93 (MQ=255)
                                                                 aaTCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGTGTCAGacac                        >  1:4282313/1‑93 (MQ=255)
                                                                 aaTCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGTGTCAGacac                        >  2:1181875/1‑93 (MQ=255)
                                                                 aaTCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGTGTCAGacac                        <  1:5745433/93‑1 (MQ=255)
                                                                  aTCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGTGTCAGACACg                       >  1:4600744/1‑93 (MQ=255)
                                                                    cGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGTGTCAGACACGAg                     <  2:3902856/93‑1 (MQ=255)
                                                                     ggTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGTGTCAGACACGAgc                    >  1:6315815/1‑93 (MQ=255)
                                                                      gTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGTGTCAGACACGAgcg                   >  2:3431897/1‑93 (MQ=255)
                                                                      gTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGTGTCAGACACGAgcg                   <  2:3856024/93‑1 (MQ=255)
                                                                       tattatCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGTGTCAGACACGAgcgc                  <  1:1456991/93‑1 (MQ=255)
                                                                       tattatCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGTGTCAGACACGAgcgc                  <  1:1584079/93‑1 (MQ=255)
                                                                       tattatCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGTGTCAGACACGAgcgc                  >  1:3769187/1‑93 (MQ=255)
                                                                       tattatCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGTGTCAGACACGAgcgc                  >  1:1944586/1‑93 (MQ=255)
                                                                        attatCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGTGTCAGACACGAGCgca                 <  2:3488030/93‑1 (MQ=255)
                                                                        attatCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGTGTCAGACACGAGCgca                 >  2:3638138/1‑93 (MQ=255)
                                                                         ttatCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGTGTCAGACACGAGCgcag                >  2:5755744/1‑93 (MQ=255)
                                                                          tatCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGTGTCAGACACGAGCgcagc               >  2:6272790/1‑93 (MQ=255)
                                                                          tatCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGTGTCAGACACGAGCgcagc               >  1:606365/1‑93 (MQ=255)
                                                                          tatCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGTGTCAGACACGAGCgcagc               <  1:2277161/93‑1 (MQ=255)
                                                                          tatCAATTTCGTTAATGGCATTGCTCATTGCACCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGTGTCAGACACGAGCgcagc               <  1:958680/93‑1 (MQ=255)
                                                                           atCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGTGTCAGACACGAGCgcagca              <  2:60698/93‑1 (MQ=255)
                                                                           atCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGTGTCAGACACGAGCgcagca              >  2:701979/1‑93 (MQ=255)
                                                                           atCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGTGTCAGACACGAGCgcagca              >  1:6098914/1‑93 (MQ=255)
                                                                           atCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGTGTCAGACACGAGCgcagca              >  2:4395740/1‑93 (MQ=255)
                                                                            tCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGTGTCAGACACGAGCGcagcag             <  2:1408411/93‑1 (MQ=255)
                                                                            tCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGTGTCAGACACGAGCGcagcag             >  1:3553014/1‑93 (MQ=255)
                                                                            tCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGTGTCAGACACGAGCGcagcag             >  1:5268487/1‑93 (MQ=255)
                                                                             cAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGTGTCAGACACGAGCGCAGCAGt            <  2:5671554/93‑1 (MQ=255)
                                                                              aaTTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGTGTCAGACACGAGCGCAGCAGTc           <  1:129035/93‑1 (MQ=255)
                                                                              aaTTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGTGTCAGACACGAGCGCAGCAGTc           >  1:172081/1‑93 (MQ=255)
                                                                              aaTTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGTGTCAGACACGAGCGCAGCAGTc           <  1:6445787/93‑1 (MQ=255)
                                                                               aTTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGTGTCAGACACGAGCGCAGCAGTCa          <  1:620912/93‑1 (MQ=255)
                                                                                  tCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGTGTCAGACACGAGCGCAGCAGTCAACg       >  1:2581130/1‑93 (MQ=255)
                                                                                  tCGTTAATGGCATCGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGTGTCAGACACGAGCGCAGCAGTCAACg       <  2:66311/93‑1 (MQ=255)
                                                                                   cGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGTGTCAGACACGAGCGCAGCAGTCAACGt      <  2:5333288/93‑1 (MQ=255)
                                                                                   cGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGTGTCAGACACGAGCGCAGCAGTCAACGt      >  1:4006468/1‑93 (MQ=255)
                                                                                     ttAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGTGTCAGACACGAGCGCAGCAGTCAACGTTa    >  2:4347368/1‑93 (MQ=255)
                                                                                       aaTGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGTGTCAGACACGAGCGCAGCAGTCAACGTTAtt  >  2:3577760/1‑93 (MQ=255)
                                                                                       aaTGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGTGTCAGACACGAGCGCAGCAGTCAACGTTAtt  >  2:3554744/1‑93 (MQ=255)
                                                                                          |                                                                                         
GTTAATTACGTGATTCGCCACGATTGGGTGGGAATCCGCCGGGTTGTACCCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATAGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGTGTCAGACACGAGCGCAGCAGTCAACGTTATT  >  NC_000913/4296376‑4296555

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGCGTTAATTACGTGATTCGCCACGATTGGGTGGGAATCCGCCGGGTTGTACCCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATAGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGTGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTA  >  NC_000913/4296373‑4296559
                                                                                             |                                                                                             
AGCGTTAATTACGTGATTCGCCACGATTGGGTGGGAATCCGCCGGGTTGTACCCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATG                                                                                               >  SRR4302534.1333290/1‑94 (MQ=60)
AGCGTTAATTACGTGATTCGCCACGATTGGGTGGGAATCCGCCGGGTTGTACCCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATG                                                                                               >  SRR4302534.4370578/1‑94 (MQ=60)
 GCGTTAATTACGTGATTCGCCACGATTGGGTGGGAATCCGCCGGGTTGTACCCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGG                                                                                              <  SRR4302534.568101/94‑1 (MQ=60)
 GCGTTAATTACGTGATTCGCCACGATTGGGTGGGAATCCGCCGGGTTGTACCCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGG                                                                                              >  SRR4302534.3050318/1‑94 (MQ=60)
  CGTTAATTACGTGATTCGCCACGATTGGGTGGGAATCCGCCGGGTTGTACCCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGC                                                                                             >  SRR4302534.1326277/1‑94 (MQ=60)
  CGTTAATTACGTGATTCGCCACGATTGGGTGGGAATCCGCCGGGTTGTACCCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGC                                                                                             <  SRR4302534.2190222/94‑1 (MQ=60)
   GTTAATTACGTGATTCGCCACGATTGGGTGGGAATCCGCCGGGTTGTACCCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCA                                                                                            >  SRR4302534.5678685/1‑94 (MQ=60)
     TAATTACGTGATTCGCCACGATTGGGTGGGAATCCGCCGGGTTGTACCCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATT                                                                                          >  SRR4302534.961475/1‑94 (MQ=60)
     TAATTACGTGATTCGCCACGATTGGGTGGGAATCCGCCGGGTTGTACCCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATT                                                                                          <  SRR4302534.1632250/94‑1 (MQ=60)
     TAATTACGTGATTCGCCACGATTGGGTGGGAATCCGCCGGGTTGTACCCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATT                                                                                          >  SRR4302534.2192502/1‑94 (MQ=60)
     TAATTACGTGATTCGCCACGATCGGGTGGGAATCCGCCGGGTTGTACCCGAAAACGAACACTAAATCGTTATTATCATTTTCGTTAATGGCATA                                                                                          <  SRR4302534.5111755/94‑1 (MQ=60)
      AATTACGTGATTCGCCACGATTGGGTGGGAATCCGCCGGGTTGTACCCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTG                                                                                         >  SRR4302534.5221801/1‑94 (MQ=60)
      AATTACGTGATTCGCCACGATTGGGTGGGAATCCGCCGGGTTGTACCCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTG                                                                                         <  SRR4302534.4730822/94‑1 (MQ=60)
      AATTACGTGATTCGCCACGATTGGGTGGGAATCCGCCGGGTTGTACCCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTG                                                                                         <  SRR4302534.4739048/94‑1 (MQ=60)
      AATTACGTGATTCGCCACGATTGGGTGGGAATCCGCCGGGTTGTACCCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTG                                                                                         <  SRR4302534.1652980/94‑1 (MQ=60)
        TTACGTGATTCTCCACGATTGGGTGGGAATCCGCCGGGTTGTACCCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCT                                                                                       <  SRR4302534.5900929/94‑1 (MQ=60)
        TTACGTGATTCGCCACGATTGGGTGGGAATCCGCCGGGTTGTACCCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCT                                                                                       <  SRR4302534.3042311/94‑1 (MQ=60)
         TACGTGATTCGCCACGATTGGGTGGGAATCCGCCGGGTTGTACCCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTC                                                                                      >  SRR4302534.992317/1‑94 (MQ=60)
         TACGTGATTCGCCACGATTGGGTGGGAATCCGCCGGGTTGTACCCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTC                                                                                      <  SRR4302534.6263116/94‑1 (MQ=60)
           CGTGATTCGCCACGATTGGGTGGGAATCCGCCGGGTTGTACCCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCAT                                                                                    <  SRR4302534.3667101/94‑1 (MQ=60)
           CGTGATTCGCCACGATTGGGTGGGAATCCGCCGGGTTGTACCCGAAAACGAACACTAAATCGGCATTATCAATTTCGTTAATGGCATTGCTCAT                                                                                    >  SRR4302534.552185/1‑94 (MQ=60)
            GTGATTCGCCACGATTGGGTGGGAATCCGCCGGGTTGTACCCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATT                                                                                   <  SRR4302534.4978181/94‑1 (MQ=60)
             TGATTCGCCACGATTGGGTGGGAATCCGCCGGGTTGTACCCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTG                                                                                  <  SRR4302534.4968011/94‑1 (MQ=60)
             TGATTCGCCACGATTGGGTGGGAATCCGCCGGGTTGTACCCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTG                                                                                  <  SRR4302534.2223047/94‑1 (MQ=60)
              GATTCGCCACGATTGGGTGGGAATCCGCCGGGTTGTACCCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGC                                                                                 >  SRR4302534.4076161/1‑94 (MQ=60)
               ATTCGCCACGATTGGGTGGGAATCCTCCGGGTTGTACCCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCG                                                                                <  SRR4302534.1982442/94‑1 (MQ=60)
               ATTCGCCACGATTGGGTGGGAATCCGCCGGGTTGTACCCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCG                                                                                >  SRR4302534.2780041/1‑94 (MQ=60)
               ATTCGCCACGATTGGGTGGGAATCCGCCGGGTTGTACCCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCG                                                                                <  SRR4302534.4468460/94‑1 (MQ=60)
                  CGCCACGATTGGGTGGGAATCCGCCGGGTTGTACCCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCA                                                                             <  SRR4302534.5126201/94‑1 (MQ=60)
                  CGCCACGATTGGGTGGGAATCCGCCGGGTTGTACCCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCA                                                                             <  SRR4302534.343628/94‑1 (MQ=60)
                     CACGATTGGGTGGGAATCCGCCGGGTTGTACCCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTA                                                                          <  SRR4302534.4980987/94‑1 (MQ=60)
                     CACGATTGGGTGGGAATCCGCCGGGTTGTACCCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTA                                                                          >  SRR4302534.3768215/1‑94 (MQ=60)
                     CACGATTGGGTGGGAATCCGCCGGGTTGTACCCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTA                                                                          <  SRR4302534.4827806/94‑1 (MQ=60)
                     CACGATTGGGTGGGAATCCGCCGGGTTGTACCCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTA                                                                          >  SRR4302534.3610239/1‑94 (MQ=60)
                      ACGATTGGGTGGGAATCCGCCGTGTTGTACCCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTAC                                                                         <  SRR4302534.6621617/94‑1 (MQ=60)
                      ACGATTGGGTGGGAATCCGCCGGGTTGTACCCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTAC                                                                         <  SRR4302534.3369300/94‑1 (MQ=60)
                       CGATTGGGTGGGAATCCGCCGGGTTGTACCCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACC                                                                        <  SRR4302534.2486222/94‑1 (MQ=60)
                       CGATTGGGTGGGAATCCGCCGGGTTGTACCCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACC                                                                        <  SRR4302534.6629018/94‑1 (MQ=60)
                       CGATTGGGTAGGAATCCGCCGGGTTGTACCCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACC                                                                        >  SRR4302534.6448985/1‑94 (MQ=60)
                        GATTGGGTGGGAATCCGCCGGGTTGTACCCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCG                                                                       >  SRR4302534.3582929/1‑94 (MQ=60)
                        GATTGGGTGGGAATCCGCCGGGTTGTACCCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCG                                                                       >  SRR4302534.852943/1‑94 (MQ=60)
                         ATTGGGTGGGAATCCGCCGGGTTGTACCCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGA                                                                      >  SRR4302534.1298709/1‑94 (MQ=60)
                          TTGGGTGGGAATCCGCCGGGTTGTACCCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGAC                                                                     <  SRR4302534.4632008/94‑1 (MQ=60)
                           TGGGTGGGAATCCGCCGGGTTGTACCCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACC                                                                    <  SRR4302534.3983019/94‑1 (MQ=60)
                            GGGTGGGAATCCGCCGGGTTGTACCCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCG                                                                   <  SRR4302534.2122790/94‑1 (MQ=60)
                            GGGGGGGAATCCGCCGGGTTGTACCCGAAAACGAACACTAAATCGGAATTATCAATTTAGTTAATGGCATTGCTCATTGCGCCATTACCGACCG                                                                   >  SRR4302534.4462048/1‑94 (MQ=60)
                              GTGGGAATCCGCCGGGTTGTACCCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGAT                                                                 >  SRR4302534.5609028/1‑94 (MQ=60)
                              GTGGGAATCCGCCGGGTTGTACCCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGAT                                                                 <  SRR4302534.904516/94‑1 (MQ=60)
                               TGGGAATCCGCCGGGTTGTACCCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATT                                                                <  SRR4302534.1270297/94‑1 (MQ=60)
                               TGGGAATCCGCCGGGTTGTACCCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGACT                                                                >  SRR4302534.5116858/1‑94 (MQ=60)
                                GGGAATCCGCCGGGTTGTACCCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTG                                                               <  SRR4302534.4256322/94‑1 (MQ=60)
                                 GGAATCCGCCGGGTTGTACCCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGG                                                              >  SRR4302534.2421023/1‑94 (MQ=60)
                                  GAATCCGCCGGGTTGTACCCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGT                                                             <  SRR4302534.1236441/94‑1 (MQ=60)
                                   AATCCGCCGGGTTGTACCCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGGATTGCTCATTGCGCCATTACCGACCGATTGGTG                                                            >  SRR4302534.1257950/1‑94 (MQ=60)
                                    ATCCGCCGGGTTGTACCCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGC                                                           <  SRR4302534.2521132/94‑1 (MQ=60)
                                     TCCGCCGGGTTGTACCCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCA                                                          <  SRR4302534.5706150/94‑1 (MQ=60)
                                       CGCCGGGTTGTACCCGAAAACGAACACTAAATCGGTTTTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGA                                                        >  SRR4302534.2396749/1‑94 (MQ=60)
                                         CCGGGTTGTACCCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACC                                                      >  SRR4302534.4391896/1‑94 (MQ=60)
                                          CGGGTTGTACCCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCT                                                     >  SRR4302534.3362396/1‑94 (MQ=60)
                                          CGGGTTGTACCCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCT                                                     >  SRR4302534.6335932/1‑94 (MQ=60)
                                          CGGGTTGTACCCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCT                                                     >  SRR4302534.1874702/1‑94 (MQ=60)
                                          CGGGTTGTAACCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCA                                                     >  SRR4302534.2189133/1‑94 (MQ=60)
                                              TTGTACCCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAA                                                 <  SRR4302534.1515416/94‑1 (MQ=60)
                                                GTACCCGAAATCGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACC                                               <  SRR4302534.3277752/94‑1 (MQ=60)
                                                GTACCCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACC                                               <  SRR4302534.1926570/94‑1 (MQ=60)
                                                GTACCCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACC                                               >  SRR4302534.5915647/1‑94 (MQ=60)
                                                GTACCCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACC                                               >  SRR4302534.3433398/1‑94 (MQ=60)
                                                GTACCCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACC                                               >  SRR4302534.47902/1‑94 (MQ=60)
                                                 TACCCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCG                                              >  SRR4302534.2804227/1‑94 (MQ=60)
                                                  ACCCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGA                                             <  SRR4302534.5173173/94‑1 (MQ=60)
                                                   CCCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGAT                                            <  SRR4302534.6187722/94‑1 (MQ=60)
                                                   CCCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGAT                                            >  SRR4302534.2066503/1‑94 (MQ=60)
                                                    CCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATG                                           >  SRR4302534.6220031/1‑94 (MQ=60)
                                                    CCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATG                                           <  SRR4302534.4327815/94‑1 (MQ=60)
                                                     CGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGG                                          <  SRR4302534.5225554/94‑1 (MQ=60)
                                                     CGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGG                                          >  SRR4302534.842711/1‑94 (MQ=60)
                                                     CGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGG                                          <  SRR4302534.1070922/94‑1 (MQ=60)
                                                      GAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGG                                         >  SRR4302534.2897345/1‑94 (MQ=60)
                                                      GAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGG                                         <  SRR4302534.742218/94‑1 (MQ=60)
                                                      CAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGCG                                         >  SRR4302534.3120270/1‑94 (MQ=60)
                                                        AAACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCC                                       <  SRR4302534.5632517/94‑1 (MQ=60)
                                                          ACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGT                                     <  SRR4302534.909896/94‑1 (MQ=60)
                                                          ACGAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGT                                     >  SRR4302534.2359249/1‑94 (MQ=60)
                                                            GAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGTGT                                   >  SRR4302534.2514213/1‑94 (MQ=60)
                                                            GAACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTAGTGCACACCTGCAACCcatggcccgcgc                                   >  SRR4302534.4390602/1‑82 (MQ=60)
                                                             AACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGTGTC                                  <  SRR4302534.160315/94‑1 (MQ=60)
                                                             AACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGTGTC                                  >  SRR4302534.4075375/1‑94 (MQ=60)
                                                              ACACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGTGTCA                                 <  SRR4302534.3642943/94‑1 (MQ=60)
                                                               CACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGTGTCAG                                >  SRR4302534.4245295/1‑94 (MQ=60)
                                                                ACTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGTGTCAGA                               <  SRR4302534.6243932/94‑1 (MQ=60)
                                                                 CTAAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGTGTCAGAC                              >  SRR4302534.2460560/1‑94 (MQ=60)
                                                                  TAAATCGGGATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGGTGGGCCGTGTCAGACA                             >  SRR4302534.2200090/1‑94 (MQ=60)
                                                                   AAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGTGTCAGACAC                            >  SRR4302534.5333792/1‑94 (MQ=60)
                                                                   AAATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGTGTCAGACAC                            >  SRR4302534.4398265/1‑94 (MQ=60)
                                                                    AATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGTGTCAGACACG                           <  SRR4302534.2951202/94‑1 (MQ=60)
                                                                    AATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGTGTCAGACACG                           >  SRR4302534.4725608/1‑94 (MQ=60)
                                                                    AATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGTGTCAGACACG                           <  SRR4302534.5901216/94‑1 (MQ=60)
                                                                     ATCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGTGTCAGACACGA                          >  SRR4302534.6527328/1‑94 (MQ=60)
                                                                      TCGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGTGTCAGACACGAG                         <  SRR4302534.631540/94‑1 (MQ=60)
                                                                       CGGTATTATCAATTTCGTTAATGTCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGTGTCAGACACGAGC                        <  SRR4302534.4157402/94‑1 (MQ=60)
                                                                       CGGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGTGTCAGACACGAGC                        >  SRR4302534.6486516/1‑94 (MQ=60)
                                                                        GGTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGTGTCAGACACGAGCG                       >  SRR4302534.5887406/1‑94 (MQ=60)
                                                                         GTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGTGTCAGACACGAGCGC                      >  SRR4302534.3870391/1‑94 (MQ=60)
                                                                         GTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGTGTCAGACACGAGCGC                      >  SRR4302534.1995455/1‑94 (MQ=60)
                                                                         GTATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGTGTCAGACACGAGCGC                      >  SRR4302534.4314849/1‑94 (MQ=60)
                                                                          TATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGTGTCAGACACGAGCGCA                     <  SRR4302534.1495315/94‑1 (MQ=60)
                                                                          TATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGTGTCAGACACGAGCGCA                     <  SRR4302534.1625513/94‑1 (MQ=60)
                                                                           ATTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGTGTCAGACACGAGCGCAG                    <  SRR4302534.2337738/94‑1 (MQ=60)
                                                                            TTATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGTGTCAGACACGAGCGCAGC                   >  SRR4302534.621121/1‑94 (MQ=60)
                                                                             TATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGTGTCAGACACGAGCGCAGCA                  <  SRR4302534.2219396/94‑1 (MQ=60)
                                                                             TATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGTGTCAGACACGAGCGCAGCA                  >  SRR4302534.3923344/1‑94 (MQ=60)
                                                                             TATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGTGTCAGACACGAGCGCAGCA                  <  SRR4302534.2337586/94‑1 (MQ=60)
                                                                             TATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGTGTCAGACACGAGCGCAGCA                  >  SRR4302534.6171735/1‑94 (MQ=60)
                                                                             TATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGTGTCAGACACGAGCGCAGCA                  >  SRR4302534.6264761/1‑94 (MQ=60)
                                                                             TATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCAGTGTCAGACACGAGCGgcgca                  >  SRR4302534.1734543/1‑89 (MQ=60)
                                                                             TATCAATTTCGTTAATGGCATTGCTCATTGCACCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGTGTCAGACACGAGCGCAGCA                  <  SRR4302534.983105/94‑1 (MQ=60)
                                                                              ATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGTGTCAGACACGAGCGCAGCAG                 >  SRR4302534.5410803/1‑94 (MQ=60)
                                                                              ATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGTGTCAGACACGAGCGCAGCAG                 <  SRR4302534.3229707/94‑1 (MQ=60)
                                                                              ATCAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGTGTCAGACACGAGCGCAGCAG                 >  SRR4302534.3648565/1‑94 (MQ=60)
                                                                                CAATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGTGTCAGACACGAGCGCAGCAGTC               >  SRR4302534.176617/1‑94 (MQ=60)
                                                                                CAATTTCGATAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGTGTCAGACACGAGCGCAGCAGTC               >  SRR4302534.6532971/1‑94 (MQ=60)
                                                                                 AATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGTGTCAGACACGAGCGCAGCAGTCA              <  SRR4302534.132448/94‑1 (MQ=60)
                                                                                 AATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGTGTCAGACACGAGCGCAGCAGTCA              <  SRR4302534.6619497/94‑1 (MQ=60)
                                                                                  ATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGTGTCAGACACGAGCGCAGCAGTCAA             <  SRR4302534.635989/94‑1 (MQ=60)
                                                                                  ATTTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGTGTCAGACACGAGCGCAGCAGTCAA             >  SRR4302534.4429683/1‑94 (MQ=60)
                                                                                    TTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGTGTCAGACACGAGCGCAGCAGTCAACG           >  SRR4302534.2649481/1‑94 (MQ=60)
                                                                                    TTCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGTGTCAGACACGAGCGCAGCAGTCAACG           >  SRR4302534.2647548/1‑94 (MQ=60)
                                                                                     TCGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGTGTCAGACACGAGCGCAGCAGTCAACGT          >  SRR4302534.4113743/1‑94 (MQ=60)
                                                                                     TCGTTAATGGCATTGCTCATTGCGCCATTAAAGACCGATTGGAGCAGCCATGCAACCAATGGGCCGTGTCAGGCCCGAGCGCAGCAGTCAACGT          <  SRR4302534.6645630/94‑1 (MQ=53)
                                                                                      CGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGTGTCAGACACGAGCGCAGCAGTCAACGTT         <  SRR4302534.5106676/94‑1 (MQ=60)
                                                                                      CGTTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGTGTCAGACACGAGCGCAGCAGTCAACGTT         <  SRR4302534.458539/94‑1 (MQ=60)
                                                                                        TTAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGTGTCAGACACGAGCGCAGCAGTCAACGTTAT       <  SRR4302534.4848064/94‑1 (MQ=60)
                                                                                         TAATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGTGTCAGACACGAGCGCAGCAGTCAACGTTATT      >  SRR4302534.4663855/1‑94 (MQ=60)
                                                                                          AATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGTGTCAGACACGAGCGCAGCAGTCAACGTTATTG     >  SRR4302534.1162723/1‑94 (MQ=60)
                                                                                          AATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGTGTCAGACACGAGCGCAGCAGTCAACGTTATTG     >  SRR4302534.1408051/1‑94 (MQ=60)
                                                                                           ATGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGTGTCAGACACGAGCGCAGCAGTCAACGTTATTGG    <  SRR4302534.1231859/94‑1 (MQ=60)
                                                                                            TGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGTGTCAGACACGAGCGCAGCAGTCAACGTTATTGGT   >  SRR4302534.6330399/1‑94 (MQ=60)
                                                                                            TGGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGTGTCAGACACGAGCGCAGCAGTCAACGTTATTGGT   <  SRR4302534.3758187/94‑1 (MQ=60)
                                                                                             GGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGTGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTA  <  SRR4302534.2391014/94‑1 (MQ=60)
                                                                                             |                                                                                             
AGCGTTAATTACGTGATTCGCCACGATTGGGTGGGAATCCGCCGGGTTGTACCCGAAAACGAACACTAAATCGGTATTATCAATTTCGTTAATAGCATTGCTCATTGCGCCATTACCGACCGATTGGTGCAGACCTGCAACCGATGGGCCGTGTCAGACACGAGCGCAGCAGTCAACGTTATTGGTA  >  NC_000913/4296373‑4296559

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑