Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 884 257.2 12958910 96.9% 12557183 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,312,334 A→G L116P (CTG→CCG)  phnP ← PhnP

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,312,3340AG99.6% 808.3 / ‑5.0 236L116P (CTG→CCG) phnPPhnP
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (126/109);  total (126/110)
Fisher's exact test for biased strand distribution p-value = 4.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.17e-01

Only 100 of 245 total aligned reads displayed.

AGTTTTGAGTGGTTGAGCGGCAGGGGCGTGACCTGTAACCCCTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCAGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGCCGTAAACCGGGATCGGAT‑CGCCAACGCCCCAGCGCAGCGGA  >  NC_000913/4312244‑4312423
                                                                                          |                                                                                          
aGTTTTGAGTGGTTGAGCGGCAGGGGCGTGACCTGTAACCCCTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCggg                                                                                          <  1:6328211/93‑1 (MQ=255)
   tttGTGTGGTTGAGCGACAGGGACGTGACCTGTAACCCCTAAAAATCAAACACCACAAACGTTTCCACCGTGTGGCTGAAATCAAGCGGGccc                                                                                       <  2:2028578/93‑1 (MQ=255)
    ttGAGTGGTTGAGCGGCAGGGGCGTGACCTGTAACCCCTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCg                                                                                      <  2:4205491/93‑1 (MQ=255)
      gAGTGGTTGAGCGGCAGGGGCGTGACCTGTAACCCCTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGa                                                                                    <  1:1529970/93‑1 (MQ=255)
         tGGTTGAGCGGCAGGGGCGTGACCTGTAACCCCTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGt                                                                                 <  1:4190722/93‑1 (MQ=255)
           gTTGAGCGGCAGGGGCGTGACCTGTAACCCCTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGttt                                                                               <  1:5083234/93‑1 (MQ=255)
            ttGAGCGGCAGGGGCGTGACCTGTAACCCCTGCAAATCAAACACCACAAACGGATCCACCGTGTGGCTGAAATCCAGAGGGTCCGGCAGTTTa                                                                              >  2:4670482/1‑93 (MQ=255)
             tGAGCGGCAGGGGCGTGACCTGTAACCCCTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTaa                                                                             <  2:2719941/93‑1 (MQ=255)
               aGCGGCAGGGGCGTGACCTGTAACCCCTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAAc                                                                           >  1:5938310/1‑93 (MQ=255)
               aGCGGCAGGGGCGTGACCTGTAACCCCTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAAc                                                                           <  1:2045594/93‑1 (MQ=255)
                gCGGCAGGGGCGTGACCTGTAACCCCTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACa                                                                          <  2:2790692/93‑1 (MQ=255)
                gCGGCAGGGGCGTGACCTGTAACCCCTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACa                                                                          <  2:3872628/93‑1 (MQ=255)
                   gCAGGGGCGTGACCTGTAACCCCTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGAt                                                                       >  2:5611258/1‑93 (MQ=255)
                   gCAGGGGCGTGACCTGTAACCCCTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCAGGCCCGGATGTTTAAACAGAt                                                                       <  2:23270/93‑1 (MQ=255)
                    gaGGGGCGTGACCTGTAACCCCTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGAtc                                                                      <  1:2579806/92‑1 (MQ=255)
                    cAGGGGCGTGACCTGTAACCCCTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGAtc                                                                      <  1:4093511/93‑1 (MQ=255)
                    cAGGGGCGTGACCTGTAACCCCTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGAtc                                                                      <  1:5395974/93‑1 (MQ=255)
                     aGGGGCGTGACCTGTAACCCCTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGAtcg                                                                     >  2:2175048/1‑93 (MQ=255)
                      ggggCGTGACCTGTAACCCCTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGAtcgt                                                                    >  2:4929157/1‑93 (MQ=255)
                      ggggCGTGACCTGTAACCCCTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGAtcgt                                                                    >  2:5716602/1‑93 (MQ=255)
                      ggggCGTGACCTGTAACCCCTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGAtcgt                                                                    <  2:4295586/93‑1 (MQ=255)
                        ggCGTGACCTGTAACCCCTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGAtcgtcg                                                                  <  2:3290346/93‑1 (MQ=255)
                         gCGTGACCTGTAACCCCTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGc                                                                 <  1:4884340/93‑1 (MQ=255)
                         gCGTGACCTGTAACCCCTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGc                                                                 <  2:1060/93‑1 (MQ=255)
                            tGACCTGTAACCCCTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGc                                                              <  1:3837444/93‑1 (MQ=255)
                             gACCTGTAACCCCTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGcc                                                             >  1:4815510/1‑93 (MQ=255)
                              aCCTGTAACCCCTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGccc                                                            <  1:6051192/93‑1 (MQ=255)
                              aCCTGTAACCCCTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGccc                                                            >  2:5312834/1‑93 (MQ=255)
                              aCCTGTAACCCCTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGccc                                                            <  1:2660544/93‑1 (MQ=255)
                               ccTGTAACCCCTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCt                                                           >  1:1832033/1‑93 (MQ=255)
                               ccTGTAACCCCTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCt                                                           <  1:1702984/93‑1 (MQ=255)
                                cTGTAACCCCTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTg                                                          >  2:3704684/1‑93 (MQ=255)
                                cTGTAACCCCTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTg                                                          <  2:450242/93‑1 (MQ=255)
                                  gTAACCCCTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGtt                                                        >  1:2804068/1‑93 (MQ=255)
                                  gTAACCCCTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGtt                                                        <  1:4587535/93‑1 (MQ=255)
                                   tAACCCCTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTc                                                       <  2:439229/93‑1 (MQ=255)
                                    aaCCCCTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCa                                                      >  1:4660044/1‑93 (MQ=255)
                                      ccccTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATc                                                    >  2:759154/1‑93 (MQ=255)
                                      ccccTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATc                                                    >  2:6204349/1‑93 (MQ=255)
                                      ccccTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATc                                                    <  1:5694188/93‑1 (MQ=255)
                                      ccccTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATc                                                    <  1:3486500/93‑1 (MQ=255)
                                       cccTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATcc                                                   <  1:2607511/93‑1 (MQ=255)
                                       cccTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGAGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATcc                                                   >  2:4106825/1‑93 (MQ=255)
                                          tGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCcggc                                                <  2:3033896/93‑1 (MQ=255)
                                          tGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCcggc                                                <  2:2528339/93‑1 (MQ=255)
                                          tGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCcggc                                                >  1:5149161/1‑93 (MQ=255)
                                            cAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCcggcgg                                              <  1:5915742/93‑1 (MQ=255)
                                            cAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCcggcgg                                              <  1:1208303/93‑1 (MQ=255)
                                            cAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCcggcgg                                              >  1:246744/1‑93 (MQ=255)
                                             aaaTCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATccggccgg                                             >  1:2181344/1‑93 (MQ=255)
                                              aaTCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGc                                            >  2:585754/1‑93 (MQ=255)
                                               aTCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGcc                                           >  2:3558019/1‑93 (MQ=255)
                                               aTCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGcc                                           >  1:4641010/1‑93 (MQ=255)
                                               aTCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAAAAGATCGTCGCAGCCCTGTTCATCCGGCGGGcc                                           >  2:6175453/1‑93 (MQ=255)
                                                 cAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGCCGt                                         >  2:4446424/1‑93 (MQ=255)
                                                 cAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGGTTAAACAGATCGTCGCATCCCTGTTCATCCGGCGGGCCGt                                         >  1:284377/1‑93 (MQ=255)
                                                  aaaCACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGCTCATCCGGCGGGCCGTa                                        >  2:578737/1‑93 (MQ=255)
                                                   aaCACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGCCGTaa                                       >  2:390017/1‑93 (MQ=255)
                                                    acacCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGCCGTaaa                                      >  2:4331622/1‑93 (MQ=255)
                                                     caccacAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGCCGTAAAc                                     <  2:1799916/93‑1 (MQ=255)
                                                       ccacAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGCCGTAAACCg                                   >  2:2591252/1‑93 (MQ=255)
                                                       ccacAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGCCGTAAACCg                                   >  2:13738/1‑93 (MQ=255)
                                                       ccacAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGCCGTAAACCg                                   >  1:3246760/1‑93 (MQ=255)
                                                         acaAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGCCGTAAACCggg                                 >  2:651932/1‑93 (MQ=255)
                                                          caAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGCCGTAAACCGgga                                <  1:6429546/93‑1 (MQ=255)
                                                           aaaCGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGCCGTAAACCGggat                               <  1:1191391/93‑1 (MQ=255)
                                                           aaaCGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGCCGTAAACCGggat                               >  1:1043180/1‑93 (MQ=255)
                                                            aaCGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGCTGTTTAAACAGATCGTCGCAGCCCTGTTCATTCCGCGGGCCGTAAACCGggatc                              >  1:3089692/1‑93 (MQ=255)
                                                            aaCGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGCCGTAAACCGggatc                              <  2:2969911/93‑1 (MQ=255)
                                                            aaCGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGCCGTAAACCGggatc                              >  2:5495016/1‑93 (MQ=255)
                                                            aaCGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGCCGTAAACCGggatc                              >  1:2928867/1‑93 (MQ=255)
                                                            aaCGGTTCCACCGTGTGGCGGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGCCGTAAACCGggatc                              <  1:3364282/93‑1 (MQ=255)
                                                             aCGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGCCGTAAACCGggatcg                             <  2:2877865/93‑1 (MQ=255)
                                                             aCGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGCCGTAAACCGggatcg                             >  2:5145594/1‑93 (MQ=255)
                                                                gTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGCCGTAAACCGggatcggat                          >  2:395257/1‑93 (MQ=255)
                                                                gTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGCCGTAAACCGggatcggat                          <  1:1387967/93‑1 (MQ=255)
                                                                 ttCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGCCGTAAACCGggatcggat‑c                        <  1:5399845/93‑1 (MQ=255)
                                                                   ccACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGCCGTAAACCGGGATCGGAT‑CGc                      >  1:854170/1‑93 (MQ=255)
                                                                   ccACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGCCGTAAACCGGGATCGGAT‑CGc                      >  1:5192640/1‑93 (MQ=255)
                                                                    cACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGCCGTAAACCGGGATCGGAT‑CGcc                     <  2:4604565/93‑1 (MQ=255)
                                                                     aCCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGCCGTAAACCGGGATCGGAT‑CGCCa                    >  2:879139/1‑93 (MQ=255)
                                                                     aCCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGCCGTAAACCGGGATCGGAT‑CGCCa                    >  2:3056912/1‑93 (MQ=255)
                                                                      ccGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGCCGTAAACCGGGATCGGAT‑CGCCaa                   >  1:219641/1‑93 (MQ=255)
                                                                       cGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGCCGTAAACCGGGATCGGAT‑CGCCAAc                  >  1:6256380/1‑93 (MQ=255)
                                                                        gtgtGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGGCGTAAACCGGGCATGGATCCGCCAAc                  >  1:157611/1‑93 (MQ=255)
                                                                           tgGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGCCGTAAACCGGGATCGGAT‑CGCCAACcccc              >  1:5276471/1‑93 (MQ=255)
                                                                            ggCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGCCGTAAACCGGGATCGGAT‑CGCCAACGcccc             <  2:2601863/93‑1 (MQ=255)
                                                                            ggCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGCCGTAAACCGGGATCGGAT‑CGCCAACGcccc             >  2:1671567/1‑93 (MQ=255)
                                                                             gCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGCCGTAAACCGGGATCGGAT‑CGCCAACGCCCca            <  1:2525137/93‑1 (MQ=255)
                                                                              cTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGCCGTAAACCGGGATCGGAT‑CGCCCACGCCCcag           >  1:686523/1‑93 (MQ=255)
                                                                              cTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGCCGTAAACCGGGATCGGAT‑CGCCAACGCCCcag           <  1:1250609/93‑1 (MQ=255)
                                                                              cTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGCCGTAAACCGGGATCGGAT‑CGCCAACGCCCcag           <  1:4040199/93‑1 (MQ=255)
                                                                              cTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGCCGTAAACCGGGATCGGAT‑CGCCAACGCCCcag           >  2:477956/1‑93 (MQ=255)
                                                                                 aaaTCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGCCGTAAACCGGGATCGGAT‑CGCCAACGCCCcagcgc        <  1:4109459/93‑1 (MQ=255)
                                                                                  aaTCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGCCGTAAACCGGGATCGGAT‑CGCCAACGCCCcagcgca       >  2:6365251/1‑93 (MQ=255)
                                                                                   aTCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGCCGTAAACCGGGATCGGAT‑CGCCAACGCCCcagcgcag      <  1:3646862/93‑1 (MQ=255)
                                                                                     cAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGCCGTAAACCGGGATCGGAT‑CGCCAACGCCCcagcgcagcg    <  1:4281970/93‑1 (MQ=255)
                                                                                      aaGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGCCGTAAACCGGGATCGGAT‑CGCCAACGCCCCAGCGCAGCgg   <  1:5062919/93‑1 (MQ=255)
                                                                                      aaGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGCCGTAAACCGGGATCGGAT‑CGCCAACGCCCCAGCGCAGCgg   >  2:248331/1‑93 (MQ=255)
                                                                                      aaGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGCCGTAAACCGGGATCGGAT‑CGCCAACGCCCCAGCGCAGCgg   >  2:3362721/1‑93 (MQ=255)
                                                                                          |                                                                                          
AGTTTTGAGTGGTTGAGCGGCAGGGGCGTGACCTGTAACCCCTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCAGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGCCGTAAACCGGGATCGGAT‑CGCCAACGCCCCAGCGCAGCGGA  >  NC_000913/4312244‑4312423

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTCAGTTTTGAGTGGTTGAGCGGCAGGGGCGTGACCTGTAACCCCTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCAGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGCCGTAAACCGGGATCGGATCGCCAACGCCCCAGCGCAGCGGAAACA  >  NC_000913/4312241‑4312427
                                                                                             |                                                                                             
GTCAGTTTTGAGTGGTTGAGCGGCAGGGGCGTGACCTGTAACCCCTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCG                                                                                               <  SRR4302534.4304360/94‑1 (MQ=60)
GTCAGTTTTGAGTGGTTGAGCGGCAGGGGCGTGACCTGTAACCCCTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCG                                                                                               <  SRR4302534.2795790/94‑1 (MQ=60)
GTCAGTTTTGAGTGGTTGAGCGGCAGGGGCGTGACCTGTAACCCCTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCG                                                                                               <  SRR4302534.6275176/94‑1 (MQ=60)
GTCAGTTTTGAGTGGTTGAGCGGCAGGGGCGTGACCTGTAACCCCTGCAAATCAAACACAACAAACGGTTCCACCGTGTTGCTGAAATCAAGCG                                                                                               <  SRR4302534.3250355/94‑1 (MQ=60)
 TCAGTTTTGAGTGGTTGAGCGGCAGGGGCGTGGCCTGTAACCCCTGCAAATCAAACACCACAAACGGATACACCGTGTGGCTGAAATCAAG‑AGG                                                                                             >  SRR4302534.3886242/1‑94 (MQ=60)
 TCAGTTTTGAGTGGTTGAGCGGCAGGGGCGTGACCTGTAACCCCTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGG                                                                                              >  SRR4302534.5502554/1‑94 (MQ=60)
   AGTTTTGAGTGGTTGAGCGGCAGGGGCGTGACCTGTAACCCCTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGC                                                                                            <  SRR4302534.6499172/94‑1 (MQ=60)
    GTTTTGAGTGGTTGAGCGGCAGGGGCGTGACCTGTAACCCCTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCC                                                                                           <  SRR4302534.1968231/94‑1 (MQ=60)
     TTTTGAGTGGTTGAGCGGCAGGGGCGTGACCTGTAACCCCTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCC                                                                                          <  SRR4302534.4023057/94‑1 (MQ=60)
      TTTGAGTGGTTGAGCGGCAGGGGCGTGACCTGTAACCCCTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCG                                                                                         >  SRR4302534.1275702/1‑94 (MQ=60)
       TTGAGTGGTTGAGCGGCAGGGGCGTGACCTGTAACCCCTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGG                                                                                        <  SRR4302534.3882136/94‑1 (MQ=60)
        TGAGTGGTTGAGCGGCAGGGGCGTGACCTGTAACCCCTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGA                                                                                       <  SRR4302534.3167322/94‑1 (MQ=60)
        TGAGTGGTTGAGCGGCAGGGGCGTGACCTGTAACCCCTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGA                                                                                       <  SRR4302534.4205374/94‑1 (MQ=60)
         GAGTGGTTGAGCGGCAGGGGCGTGACCTGTAACCCCTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGAT                                                                                      <  SRR4302534.1570112/94‑1 (MQ=60)
         GAGTGGTTGAGCGGCAGGGGCGTGACCTGTAACCCCTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGAT                                                                                      >  SRR4302534.5274454/1‑94 (MQ=60)
          AGTGGTTGAGCGGCAGGGGCGTGACCTGTAACCCCTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATG                                                                                     <  SRR4302534.2386636/94‑1 (MQ=60)
          AGTGGTTGAGCGGCAGGGGCGTGACCTGTAACCCCTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATG                                                                                     <  SRR4302534.4703902/94‑1 (MQ=60)
            TGGTTGAGCGGCAGGGGCGTGACCTGTAACCCCTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTT                                                                                   <  SRR4302534.4303941/94‑1 (MQ=60)
              GTTGAGCGGCAGGGGCGTGACCTGTAACCCCTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTA                                                                                 >  SRR4302534.3204058/1‑94 (MQ=60)
              GTTGAGCGGCAGGGGCGTGACCTGTAACCCCTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTA                                                                                 <  SRR4302534.5220433/94‑1 (MQ=60)
                 GAGCGGCAGGGGCGTGACCTGTAACCCCTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAAC                                                                              >  SRR4302534.6099555/1‑94 (MQ=60)
                  AGCGGCAGGGGCGTGACCTGTAACCCCTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACA                                                                             <  SRR4302534.1830552/94‑1 (MQ=60)
                  AGCGGCAGGGGCGTGACCTGTAACCCCTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACA                                                                             <  SRR4302534.2099061/94‑1 (MQ=60)
                    CGGCAGGGGCGTGACCTGTAACCCCTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGA                                                                           <  SRR4302534.1238620/94‑1 (MQ=60)
                      GCAGGGGCGTGACCTGTAACCCCTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATC                                                                         <  SRR4302534.6086352/94‑1 (MQ=60)
                      GCAGGGGCGTGACCTGTAACCCCTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATC                                                                         <  SRR4302534.2597148/94‑1 (MQ=60)
                       GAGGGGCGTGACCTGTAACCCCTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCG                                                                        <  SRR4302534.2648127/94‑1 (MQ=60)
                       CAGGGGCGTGACCTGTAACCCCTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCG                                                                        <  SRR4302534.5541260/94‑1 (MQ=60)
                       CAGGGGCGTGACCTGTAACCCCTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCG                                                                        <  SRR4302534.4203513/94‑1 (MQ=60)
                       CAGGGGCGTGACCTGTAACCCCTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCG                                                                        >  SRR4302534.2243535/1‑94 (MQ=60)
                       CAGGGGCGTGACCTGTAACCCCTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCG                                                                        <  SRR4302534.1463040/94‑1 (MQ=60)
                         GGGGCGTGACCTGTAACCCCTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTC                                                                      >  SRR4302534.3737880/1‑94 (MQ=60)
                         GGGGCGTGACCTGTAACCCCTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTC                                                                      <  SRR4302534.5684585/94‑1 (MQ=60)
                          GGGCGTGACCTGTAACCCCTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCG                                                                     <  SRR4302534.1245808/94‑1 (MQ=60)
                          GGGCGTGACCTGTAACCCCTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCG                                                                     <  SRR4302534.5290240/94‑1 (MQ=60)
                          GGGCGTGACCTGTAACCCCTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCG                                                                     <  SRR4302534.2130157/94‑1 (MQ=60)
                            GCGTGACCTGTAACCCCTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCA                                                                   <  SRR4302534.5016807/94‑1 (MQ=60)
                            GCGTGACCTGTAACCCCTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCA                                                                   <  SRR4302534.6085041/94‑1 (MQ=60)
                             CGTGACCTGTAACCCCTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAG                                                                  >  SRR4302534.302039/1‑94 (MQ=60)
                               TGACCTGTAACCCCTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCC                                                                >  SRR4302534.4946226/1‑94 (MQ=60)
                               TGACCTGTAACCCCTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCC                                                                <  SRR4302534.2917959/94‑1 (MQ=60)
                               TGACCTGTAACCCCTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCC                                                                <  SRR4302534.3940515/94‑1 (MQ=60)
                               TGACCTGTAACCCCTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCC                                                                >  SRR4302534.5272982/1‑94 (MQ=60)
                                GACCTGTAACCCCTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCC                                                               <  SRR4302534.5544758/94‑1 (MQ=60)
                                GACCTGTAACCCCTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCC                                                               >  SRR4302534.279055/1‑94 (MQ=60)
                                 ACCTGTACCCCCTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCT                                                              <  SRR4302534.2599063/94‑1 (MQ=60)
                                 ACCTGTAACCCCTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCT                                                              <  SRR4302534.2730887/94‑1 (MQ=60)
                                 ACCTGTAACCCCTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCT                                                              <  SRR4302534.6215717/94‑1 (MQ=60)
                                 ACCTGTAACCCCTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCT                                                              <  SRR4302534.3580101/94‑1 (MQ=60)
                                 ACCTGTAACCCCTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCT                                                              >  SRR4302534.1879675/1‑94 (MQ=60)
                                  CCTGTAACCCCTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTG                                                             >  SRR4302534.137176/1‑94 (MQ=60)
                                  CCTGTAACCCCTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTG                                                             <  SRR4302534.1747238/94‑1 (MQ=60)
                                    TGTAACCCCTGCAAATGAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTT                                                           <  SRR4302534.1744243/94‑1 (MQ=60)
                                    TGTAACCCCTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTT                                                           >  SRR4302534.2877826/1‑94 (MQ=60)
                                     GTAACCCCTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTC                                                          <  SRR4302534.4712024/94‑1 (MQ=60)
                                      TAACCCCTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCA                                                         >  SRR4302534.5767927/1‑94 (MQ=60)
                                      TAACCCCTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCA                                                         >  SRR4302534.4786573/1‑94 (MQ=60)
                                      CAACCCCTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCA                                                         <  SRR4302534.1193641/94‑1 (MQ=60)
                                        ACCCCTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATC                                                       >  SRR4302534.633662/1‑94 (MQ=60)
                                         CCCCTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCC                                                      <  SRR4302534.5203147/94‑1 (MQ=60)
                                         CCCCTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCC                                                      <  SRR4302534.5848432/94‑1 (MQ=60)
                                         CCCCTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCC                                                      <  SRR4302534.3580211/94‑1 (MQ=60)
                                          CCCTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCG                                                     <  SRR4302534.2676509/94‑1 (MQ=60)
                                          CCCTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCG                                                     <  SRR4302534.3355939/94‑1 (MQ=60)
                                          CCCTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCG                                                     >  SRR4302534.5876432/1‑94 (MQ=60)
                                           CCTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGG                                                    >  SRR4302534.4851533/1‑94 (MQ=60)
                                            CTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGC                                                   >  SRR4302534.4313233/1‑94 (MQ=60)
                                            CTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGC                                                   <  SRR4302534.6609975/94‑1 (MQ=60)
                                            CTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGC                                                   >  SRR4302534.5288082/1‑94 (MQ=60)
                                             TGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCG                                                  >  SRR4302534.4750517/1‑94 (MQ=60)
                                              atctatagattACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGG                                                 <  SRR4302534.4732296/83‑1 (MQ=60)
                                              GCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGG                                                 <  SRR4302534.5413399/94‑1 (MQ=60)
                                              GCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGG                                                 >  SRR4302534.253193/1‑94 (MQ=60)
                                               CAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGG                                                <  SRR4302534.1606065/94‑1 (MQ=60)
                                               CAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGG                                                <  SRR4302534.1240098/94‑1 (MQ=60)
                                               CAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGG                                                <  SRR4302534.6076380/94‑1 (MQ=60)
                                               CAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCCGG                                                >  SRR4302534.2239001/1‑94 (MQ=60)
                                                AAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGC                                               >  SRR4302534.622823/1‑94 (MQ=60)
                                                 AATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGCC                                              >  SRR4302534.2558648/1‑94 (MQ=60)
                                                 AATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGCC                                              >  SRR4302534.4766945/1‑94 (MQ=60)
                                                  ATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGCCG                                             <  SRR4302534.4181869/94‑1 (MQ=60)
                                                   TCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGGTTAAACAGATCGTCGCATCCCTGTTCATCCGGCGGGCCGT                                            >  SRR4302534.291730/1‑94 (MQ=60)
                                                     AAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGCCGTAA                                          >  SRR4302534.4922472/1‑94 (MQ=60)
                                                     AAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGCCGTAA                                          <  SRR4302534.2034722/94‑1 (MQ=60)
                                                     AAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGCCGTAA                                          >  SRR4302534.535001/1‑94 (MQ=60)
                                                     AAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGCCGTAA                                          >  SRR4302534.6430843/1‑94 (MQ=60)
                                                         ACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGCCGTAAACCG                                      >  SRR4302534.3333206/1‑94 (MQ=60)
                                                            ACAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGCCGTAAACCGGGA                                   <  SRR4302534.4774776/94‑1 (MQ=60)
                                                             CAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGCCGTAAACCGGGAT                                  <  SRR4302534.6602904/94‑1 (MQ=60)
                                                             CAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGCCGTAAACCGGGAT                                  >  SRR4302534.4960228/1‑94 (MQ=60)
                                                             CAAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGCCGTAAACCGGGAT                                  >  SRR4302534.1070118/1‑94 (MQ=60)
                                                              AAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGCTGTTTAAACAGATCGTCGCAGCCCTGTTCATTCCGCGGGCCGTAAACCGGGATC                                 >  SRR4302534.3170929/1‑94 (MQ=60)
                                                              AAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGCCGTAAACCGGGATC                                 >  SRR4302534.3005766/1‑94 (MQ=60)
                                                              AAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGCCGTAAACCGGGATC                                 <  SRR4302534.1222725/94‑1 (MQ=60)
                                                              AAACGGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGCCGTAAACCGGGATC                                 >  SRR4302534.943205/1‑94 (MQ=60)
                                                               AACGGTTCCACCGTGTGGCGGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGCCGTAAACCGGGATCG                                <  SRR4302534.3454393/94‑1 (MQ=60)
                                                                  GGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGCCGTAAACCGGGATCGGAT                             <  SRR4302534.6506266/94‑1 (MQ=60)
                                                                  GGTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGCCGTAAACCGGGATCGGAT                             >  SRR4302534.299294/1‑94 (MQ=60)
                                                                  GGTTCCACCGTGGGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGCCGTAAACCGGGATCGGAT                             >  SRR4302534.4292722/1‑94 (MQ=60)
                                                                   GTTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGCCGTAAACCGGGATCGGATC                            <  SRR4302534.1424497/94‑1 (MQ=60)
                                                                    TTCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGCCGTAAACCGGGATCGGATCG                           <  SRR4302534.5545208/94‑1 (MQ=60)
                                                                     TCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGCCGTAAACCGGGATCGGATCGC                          >  SRR4302534.875721/1‑94 (MQ=60)
                                                                     TCCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGCCGTAAACCGGGATCGGATCGC                          >  SRR4302534.5332637/1‑94 (MQ=60)
                                                                      CCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGCCGTAAACCGGGATCGGATCGCC                         >  SRR4302534.6151044/1‑94 (MQ=60)
                                                                      CCACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGCCGTAAACCGGGATCGGATCGCC                         >  SRR4302534.5504860/1‑94 (MQ=60)
                                                                        ACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGCCGTAAAGCGGGATCGGATCGCCAA                       >  SRR4302534.4200996/1‑94 (MQ=60)
                                                                        ACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGCCGTAAACCGGGATCGGATCGCCAA                       >  SRR4302534.225402/1‑94 (MQ=60)
                                                                        ACCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGCCGTAAACCGGGATCGGATCGCCAA                       >  SRR4302534.2915173/1‑94 (MQ=60)
                                                                         CCGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGCCGTAAACCGGGATCGGATCGCCAAC                      >  SRR4302534.6425930/1‑94 (MQ=60)
                                                                          CGTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGGCGTAAACCGGGcatggatccgccaac                     >  SRR4302534.161741/1‑79 (MQ=60)
                                                                          CGTGTGGCTGAAATCAAGCGGGCCAGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGCCGTAAACCGGGATCGGATCGCCAACG                     <  SRR4302534.2721990/94‑1 (MQ=60)
                                                                           GTGTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGCCGTAAACCGGGATCGGATCGCCAACGC                    >  SRR4302534.272319/1‑94 (MQ=60)
                                                                            tctgcCTGAAATCAAGCGCGACCGGATGTTAAAACAGCTCGTCGCAGCCCTGTTCATCCGGCGGGCCGTAAACCGGGATCGGATCGCCAACGCC                   <  SRR4302534.615937/89‑1 (MQ=60)
                                                                             GTGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGCCGTAAACCGGGATCGGATCGCCAACCCCC                  >  SRR4302534.5418948/1‑94 (MQ=60)
                                                                              TGGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGCCGTAAACCGGGATCGGATCGCCAACGCCCC                 >  SRR4302534.5125864/1‑94 (MQ=60)
                                                                               GGCTGAATTCAAGCGGACCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCGGGGGGGCCGTAAACCGGGATCGGATCGCCAACGCCCCA                <  SRR4302534.4916349/94‑1 (MQ=60)
                                                                               GGCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGCCGTAAACCGGGATCGGATCGCCCACGCCCCA                >  SRR4302534.4220013/1‑94 (MQ=60)
                                                                                GCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGCCGTAAACCGGGATCGGATCGCCCACGCCCCAG               >  SRR4302534.703297/1‑94 (MQ=60)
                                                                                GCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGCCGTAAACCGGGATCGGATCGCCAACGCCCCAG               <  SRR4302534.2592147/94‑1 (MQ=60)
                                                                                GCTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGCCGTAAACCGGGATCGGATCGCCAACGCCCCAG               <  SRR4302534.265698/94‑1 (MQ=60)
                                                                                 CTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGCCGTAAACCGGGATCGGATCGCCAACGCCCCAGC              <  SRR4302534.3812436/94‑1 (MQ=60)
                                                                                 CTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGCCGTAAACCGGGATCGGATCGCCAACGCCCCAGC              <  SRR4302534.1283570/94‑1 (MQ=60)
                                                                                 CTGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGCCGTAAACCGGGATCGGATCGCCAACGCCCCAGC              <  SRR4302534.4148530/94‑1 (MQ=60)
                                                                                  TGAAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGCCGTAAACCGGGATCGGATCGCCAACGCCCCAGCG             <  SRR4302534.6534336/94‑1 (MQ=60)
                                                                                    AAATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGCCGTAAACCGGGATCGGATCGCCAACGCCCCAGCGCA           <  SRR4302534.4219994/94‑1 (MQ=60)
                                                                                     AATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGCCGTAAACCGGGATCGGATCGCCAACGCCCCAGCGCAG          >  SRR4302534.169389/1‑94 (MQ=60)
                                                                                     AATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGCCGTAAACCGGGATCGGATCGCCAACGCCCCAGCGCAG          >  SRR4302534.2365126/1‑94 (MQ=60)
                                                                                      ATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGCCGTAAACCGGGATCGGATCGCCAACGCCCCAGCGCAGC         <  SRR4302534.3475611/94‑1 (MQ=60)
                                                                                      ATCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGCCGTAAACCGGGATCGGATCGCCAACGCCCCAGCGCAGC         <  SRR4302534.3744852/94‑1 (MQ=60)
                                                                                       TCAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGCCGTAAACCGGGATCGGATCGCCAACGCCCCAGCGCAGCG        >  SRR4302534.726099/1‑94 (MQ=60)
                                                                                        CAAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGCCGTAAACCGGGATCGGATCGCCAACGCCCCAGCGCAGCGG       <  SRR4302534.4397908/94‑1 (MQ=60)
                                                                                         AAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGCCGTAAACCGGGATCGGATCGCCAACGCCCCAGCGCAGCGGA      <  SRR4302534.5199632/94‑1 (MQ=60)
                                                                                         AAGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGCCGTAAACCGGGATCGGATCGCAAACGCCCCAACGCAGCGGA      >  SRR4302534.5170103/1‑94 (MQ=60)
                                                                                          AGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGCCGTAAACCGGGATCGGATCGCCAACGCCCCAGCGCAGCGGAA     <  SRR4302534.1511252/94‑1 (MQ=60)
                                                                                          AGCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGCCGTAAACCGGGATCGGATCGCCAACGCCCCAGCGCAGCGGAA     >  SRR4302534.2138982/1‑94 (MQ=60)
                                                                                           GCGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGCCGTAAACCGGGATCGGATCGCCAACGCCCCAGCGCAGCGGAAA    >  SRR4302534.1071597/1‑94 (MQ=60)
                                                                                            CGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGCCGTAAACCGGGATCGGATCGCCAACGCCCCAGCGCAGCGGAAAC   <  SRR4302534.574443/94‑1 (MQ=60)
                                                                                            CGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGCCGTAAACCGGGATCGGATCGCCAACGCCCCAGCGCAGCGGAAAC   <  SRR4302534.2062401/94‑1 (MQ=60)
                                                                                            CGGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGCCGTAAACCGGGATCGGATCGCCAACGCCCCAGCGCAGCGGAAAC   >  SRR4302534.1436367/1‑94 (MQ=60)
                                                                                             GGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGCCGTAAACCGGGATCGGATCGCCAACGCCCCAGCGCAGCGGAAACA  <  SRR4302534.969655/94‑1 (MQ=60)
                                                                                             GGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGCCGTAAACCGGGATCGGATCGCCAACCCCCCAGCGCAGCGGAAAAA  >  SRR4302534.3228160/1‑94 (MQ=60)
                                                                                             GGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGCCGTAAAACGGGATCGGATCGCCAAAGCCCCAGCGCAGCGGGAACA  >  SRR4302534.6361194/1‑94 (MQ=60)
                                                                                             |                                                                                             
GTCAGTTTTGAGTGGTTGAGCGGCAGGGGCGTGACCTGTAACCCCTGCAAATCAAACACCACAAACGGTTCCACCGTGTGGCTGAAATCAAGCAGGCCCGGATGTTTAAACAGATCGTCGCAGCCCTGTTCATCCGGCGGGCCGTAAACCGGGATCGGATCGCCAACGCCCCAGCGCAGCGGAAACA  >  NC_000913/4312241‑4312427

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑