Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 884 257.2 12958910 96.9% 12557183 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,334,019 A→G L197L (CTT→CTC)  yjdE ← putative amino acid/amine transport protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,334,0190AG99.2% 884.9 / ‑2.9 257L197L (CTT→CTC) yjdEputative amino acid/amine transport protein
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base G (144/111);  total (145/112)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.09e-01

Only 100 of 266 total aligned reads displayed.

GGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGGAACCAGAACCAGCCAAA  >  NC_000913/4333929‑4334108
                                                                                          |                                                                                         
ggCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAg                                                                                         >  1:1904903/1‑93 (MQ=255)
  cGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAggg                                                                                       <  2:4262681/93‑1 (MQ=255)
   gATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGt                                                                                      >  1:3446862/1‑93 (MQ=255)
   gATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGt                                                                                      >  2:263664/1‑93 (MQ=255)
    aTAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTa                                                                                     >  1:4291178/1‑93 (MQ=255)
    aTAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTa                                                                                     >  2:6121489/1‑93 (MQ=255)
     tAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTAc                                                                                    <  2:6206505/93‑1 (MQ=255)
     tAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTAc                                                                                    >  2:1786082/1‑93 (MQ=255)
     tAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTAc                                                                                    >  2:4722556/1‑93 (MQ=255)
      aGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACt                                                                                   <  2:708472/93‑1 (MQ=255)
      aGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACt                                                                                   >  1:4534974/1‑93 (MQ=255)
      aGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACt                                                                                   <  1:2384079/93‑1 (MQ=255)
       gggACATTGCGTTTCGGGTTTTTCACCACGCCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACtt                                                                                  <  2:1037015/93‑1 (MQ=255)
       gggACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACtt                                                                                  >  1:447480/1‑93 (MQ=255)
       gggACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACtt                                                                                  >  1:487243/1‑93 (MQ=255)
       gggACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACtt                                                                                  >  1:3868846/1‑93 (MQ=255)
        ggACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACttt                                                                                 >  1:4580897/1‑93 (MQ=255)
        ggACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACttt                                                                                 >  2:3483926/1‑93 (MQ=255)
           cATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGaa                                                                              <  2:5325765/93‑1 (MQ=255)
             ttGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAAtt                                                                            <  1:5959486/93‑1 (MQ=255)
               tcGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGc                                                                          <  1:2071040/92‑1 (MQ=255)
                cGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCa                                                                         <  2:1449815/93‑1 (MQ=255)
                cGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCa                                                                         <  2:3811294/93‑1 (MQ=255)
                 gTTTCGGGTTTTTCACCACACCTGCCGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCCTAACGTTGAGGGTACTTTGAATTGCAc                                                                        >  1:6071993/1‑93 (MQ=255)
                  tttCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCAcc                                                                       >  1:3571894/1‑93 (MQ=255)
                      gggTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAg                                                                   <  2:2153839/93‑1 (MQ=255)
                      gggTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGAACTTTGAATTGCACCGAAg                                                                   >  2:4881250/1‑93 (MQ=255)
                       ggTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAgg                                                                  <  2:1349679/93‑1 (MQ=255)
                       ggTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAgg                                                                  <  1:3349429/93‑1 (MQ=255)
                        gTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGt                                                                 <  1:2724446/93‑1 (MQ=255)
                             tCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCa                                                            <  1:2669493/93‑1 (MQ=255)
                              taccacACCCGCGGCAGCGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAg                                                           <  1:5067417/92‑1 (MQ=255)
                              caccacACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAg                                                           <  1:6247134/93‑1 (MQ=255)
                              caccacACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAg                                                           <  1:1270349/93‑1 (MQ=255)
                                ccacACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGc                                                         >  1:1060835/1‑93 (MQ=255)
                                 cacaCCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGcc                                                        >  2:482452/1‑93 (MQ=255)
                                 cacaCCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAAGcc                                                        >  2:3902442/1‑93 (MQ=255)
                                   cacCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGc                                                      >  2:2317532/1‑93 (MQ=255)
                                   cacCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGc                                                      >  2:5131444/1‑93 (MQ=255)
                                     ccTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTg                                                    <  2:1526873/93‑1 (MQ=255)
                                     ccTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTg                                                    <  2:2398592/93‑1 (MQ=255)
                                     ccTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTg                                                    >  2:2776974/1‑93 (MQ=255)
                                      cTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGGCGCTGa                                                   <  2:5014425/93‑1 (MQ=255)
                                       tGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGAc                                                  >  1:2031113/1‑93 (MQ=255)
                                       tGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGAc                                                  >  1:411393/1‑93 (MQ=255)
                                       tGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGAc                                                  <  2:2830846/93‑1 (MQ=255)
                                         cGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGt                                                >  1:2850869/1‑93 (MQ=255)
                                         cGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGt                                                >  2:477644/1‑93 (MQ=255)
                                          ggCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGtt                                               >  1:3295492/1‑93 (MQ=255)
                                           gCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTc                                              >  1:6468466/1‑93 (MQ=255)
                                           gCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTc                                              <  2:1220901/93‑1 (MQ=255)
                                            cAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTcc                                             <  1:1339069/93‑1 (MQ=255)
                                            cAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTcc                                             <  1:1828731/93‑1 (MQ=255)
                                             aaCGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCa                                            <  2:6004908/93‑1 (MQ=255)
                                              aCGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCAt                                           >  1:2802851/1‑93 (MQ=255)
                                              aCGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCAt                                           >  2:3252234/1‑93 (MQ=255)
                                              aCGGAAGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCAt                                           >  1:4072101/1‑93 (MQ=255)
                                               cGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATg                                          >  1:302997/1‑93 (MQ=255)
                                                ggAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATgc                                         >  1:4679900/1‑93 (MQ=255)
                                                 gAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATgcc                                        <  2:5256403/93‑1 (MQ=255)
                                                  aGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATgccg                                       >  2:4968443/1‑93 (MQ=255)
                                                  aGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATgccg                                       >  1:4340334/1‑93 (MQ=255)
                                                  aGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATgccg                                       >  1:4978137/1‑93 (MQ=255)
                                                  aGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATgccg                                       >  1:5185386/1‑93 (MQ=255)
                                                   ggCACTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATgccgc                                      <  2:5791631/93‑1 (MQ=255)
                                                     cACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCa                                    >  2:3083750/1‑93 (MQ=255)
                                                        tttCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCatat                                 >  2:4413027/1‑93 (MQ=255)
                                                         ttCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCAtata                                <  1:3592696/93‑1 (MQ=255)
                                                           ccACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAgg                              >  1:2274901/1‑93 (MQ=255)
                                                           ccACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAgg                              >  1:3444245/1‑93 (MQ=255)
                                                            cacaCCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGt                             <  1:316729/93‑1 (MQ=255)
                                                             acacCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGtt                            <  1:4651097/93‑1 (MQ=255)
                                                             acacCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGtt                            >  2:1868354/1‑93 (MQ=255)
                                                              cacCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGttt                           >  1:956323/1‑93 (MQ=255)
                                                              cacCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGttt                           <  2:3779809/93‑1 (MQ=255)
                                                               cccGATGAAACACCCCAACGTCACGTTGAGGGTACTTTGAATTGCCCCGAAGGGGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTc                          <  2:2907293/92‑1 (MQ=255)
                                                                ccGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTACACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTca                         >  2:4531635/1‑93 (MQ=255)
                                                                 cGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTcac                        <  2:5544073/93‑1 (MQ=255)
                                                                  gATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTcacc                       >  1:902059/1‑93 (MQ=255)
                                                                   aTGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTcacca                      >  1:2607931/1‑93 (MQ=255)
                                                                   aTGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTcacca                      >  1:391649/1‑93 (MQ=255)
                                                                    tGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTcaccac                     >  2:1547835/1‑93 (MQ=255)
                                                                     gAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACg                    >  2:4027043/1‑93 (MQ=255)
                                                                         gACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGgaac                >  1:5370856/1‑93 (MQ=255)
                                                                          aCCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGgaacc               <  2:6448246/93‑1 (MQ=255)
                                                                          aCCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGgaacc               >  1:1461365/1‑93 (MQ=255)
                                                                          aCCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGgaacc               >  2:3950135/1‑93 (MQ=255)
                                                                          aCCACAGCGTAACGTGGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGgaacc               <  2:5656565/93‑1 (MQ=255)
                                                                           ccACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGgaacca              <  2:2513474/93‑1 (MQ=255)
                                                                           ccACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGgaacca              >  1:587304/1‑93 (MQ=255)
                                                                            cacaGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGgaaccag             >  2:5221732/1‑93 (MQ=255)
                                                                            cacaGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGgaaccag             >  2:666828/1‑93 (MQ=255)
                                                                             acaGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACTgaaccaga            <  1:1001247/93‑1 (MQ=255)
                                                                             acaGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGgaaccaga            >  2:2967357/1‑93 (MQ=255)
                                                                               aGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGgaaccagaac          <  1:1627087/93‑1 (MQ=255)
                                                                               aGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGgaaccagaac          >  1:1192758/1‑93 (MQ=255)
                                                                                  gTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGGaaccagaaccag       >  1:5594167/1‑93 (MQ=255)
                                                                                   tAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGGAACCAGAACCAGc      >  1:1457513/1‑93 (MQ=255)
                                                                                    aaCGTTGAGGGAACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGGAACCAGAACCAGcc     >  2:3214948/1‑93 (MQ=255)
                                                                                       gTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGGAACCAGAACCAGCCaaa  >  1:6318040/1‑93 (MQ=255)
                                                                                          |                                                                                         
GGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGGAACCAGAACCAGCCAAA  >  NC_000913/4333929‑4334108

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGGAACCAGAACCAGCCAAATACG  >  NC_000913/4333926‑4334112
                                                                                             |                                                                                             
GGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTG                                                                                               >  SRR4302534.1851583/1‑94 (MQ=60)
GGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTG                                                                                               <  SRR4302534.878784/94‑1 (MQ=60)
 GTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGA                                                                                              <  SRR4302534.1521403/94‑1 (MQ=60)
  TGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGTGTAACGTTGAG                                                                                             <  SRR4302534.4689523/94‑1 (MQ=60)
  TGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAG                                                                                             >  SRR4302534.1954597/1‑94 (MQ=60)
  TGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAG                                                                                             >  SRR4302534.5901897/1‑94 (MQ=60)
  TGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAG                                                                                             <  SRR4302534.1613263/94‑1 (MQ=60)
   GGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGG                                                                                            <  SRR4302534.2382414/94‑1 (MQ=60)
     CGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGT                                                                                          >  SRR4302534.2729419/1‑94 (MQ=60)
     CGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGT                                                                                          >  SRR4302534.3539506/1‑94 (MQ=60)
      GATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTA                                                                                         >  SRR4302534.4407443/1‑94 (MQ=60)
      GATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTA                                                                                         >  SRR4302534.738677/1‑94 (MQ=60)
      GATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTA                                                                                         >  SRR4302534.6621849/1‑94 (MQ=60)
        TAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACT                                                                                       >  SRR4302534.4260997/1‑94 (MQ=60)
        TAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACT                                                                                       >  SRR4302534.4657867/1‑94 (MQ=60)
         AGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTT                                                                                      >  SRR4302534.458822/1‑94 (MQ=60)
         AGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTT                                                                                      <  SRR4302534.2447426/94‑1 (MQ=60)
         AGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTT                                                                                      >  SRR4302534.499434/1‑94 (MQ=60)
         AGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTT                                                                                      >  SRR4302534.3972725/1‑94 (MQ=60)
         AGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTT                                                                                      >  SRR4302534.1019346/1‑94 (MQ=60)
          GGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTT                                                                                     <  SRR4302534.3497822/94‑1 (MQ=60)
          GGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTT                                                                                     >  SRR4302534.4705165/1‑94 (MQ=60)
            GACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGA                                                                                   >  SRR4302534.3967581/1‑94 (MQ=60)
                TTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTG                                                                               <  SRR4302534.6121294/94‑1 (MQ=60)
                TTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAAGGGACTTTGAATTG                                                                               >  SRR4302534.1555556/1‑94 (MQ=60)
                 TGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGC                                                                              <  SRR4302534.4783220/94‑1 (MQ=60)
                  TCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCA                                                                             <  SRR4302534.2125211/94‑1 (MQ=60)
                  GCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCA                                                                             >  SRR4302534.3401760/1‑94 (MQ=60)
                   CGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCAC                                                                            >  SRR4302534.4698727/1‑94 (MQ=60)
                   CGTTTCGGGTTTTTCACCACACCTGCCGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCCTAACGTTGAGGGTACTTTGAATTGCAC                                                                            >  SRR4302534.6237086/1‑94 (MQ=60)
                    GTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACC                                                                           >  SRR4302534.3667971/1‑94 (MQ=60)
                    GTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACC                                                                           >  SRR4302534.6208199/1‑94 (MQ=60)
                    GTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACC                                                                           >  SRR4302534.1311439/1‑94 (MQ=60)
                      TTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGA                                                                         >  SRR4302534.2955078/1‑94 (MQ=60)
                      TTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGA                                                                         <  SRR4302534.4152279/94‑1 (MQ=60)
                         GGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGG                                                                      >  SRR4302534.3682731/1‑94 (MQ=60)
                         GGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGG                                                                      <  SRR4302534.2601158/94‑1 (MQ=60)
                          GGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGT                                                                     <  SRR4302534.2375656/94‑1 (MQ=60)
                          GGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGT                                                                     <  SRR4302534.3439111/94‑1 (MQ=60)
                          GGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGT                                                                     <  SRR4302534.6432738/94‑1 (MQ=60)
                           GTTTTTCACCCCACCTGCGGCAACGGAGTCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTG                                                                    <  SRR4302534.1409995/94‑1 (MQ=60)
                           GTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTG                                                                    <  SRR4302534.2796319/94‑1 (MQ=60)
                           GTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTG                                                                    <  SRR4302534.6319268/94‑1 (MQ=60)
                            TTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGC                                                                   >  SRR4302534.4199249/1‑94 (MQ=60)
                               TTCACCACACCTGCGGTAACGGAGGCACTTTCCACACCGATGACCGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCGCCGAAGGTGCCCA                                                                <  SRR4302534.399720/94‑1 (MQ=60)
                               TTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCA                                                                <  SRR4302534.5462585/94‑1 (MQ=60)
                               TTCACCACACCTGCGGCAACGGAGCCACTTTCCACACCGATGAACGACCACAGCGTACCGTTGAGGTTCCTTTAAATGGCACCGAAGGTGCCCA                                                                <  SRR4302534.320073/94‑1 (MQ=60)
                                TCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAG                                                               <  SRR4302534.2740048/94‑1 (MQ=60)
                                 TACCACACCCGCGGCAGCGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGG                                                              <  SRR4302534.5204226/94‑1 (MQ=60)
                                 CACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGG                                                              <  SRR4302534.1303808/94‑1 (MQ=60)
                                 CACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGG                                                              <  SRR4302534.5204224/94‑1 (MQ=60)
                                 CACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGG                                                              <  SRR4302534.6416566/94‑1 (MQ=60)
                                 CACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGCTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGG                                                              >  SRR4302534.5185288/1‑94 (MQ=60)
                                  CCCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTACCCAGGC                                                             <  SRR4302534.6217027/94‑1 (MQ=60)
                                  ACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGC                                                             >  SRR4302534.1088330/1‑94 (MQ=60)
                                    CACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCG                                                           >  SRR4302534.1496755/1‑94 (MQ=60)
                                    CACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCG                                                           <  SRR4302534.2753948/94‑1 (MQ=60)
                                    CACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCG                                                           <  SRR4302534.4905639/94‑1 (MQ=60)
                                    CACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCG                                                           <  SRR4302534.3128369/94‑1 (MQ=60)
                                    CACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCG                                                           >  SRR4302534.6011999/1‑94 (MQ=60)
                                       ACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTG                                                        <  SRR4302534.5231788/94‑1 (MQ=60)
                                        CCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGA                                                       >  SRR4302534.3533595/1‑94 (MQ=60)
                                         CTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGAC                                                      >  SRR4302534.2084227/1‑94 (MQ=60)
                                         CTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGAC                                                      >  SRR4302534.421911/1‑94 (MQ=60)
                                         CGGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTTAATGGCACCGAAGGTGCCCAGGCCGCTGAC                                                      <  SRR4302534.1222913/94‑1 (MQ=60)
                                          TGCGGCAACGGAGTCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTCCTTTGAATTGCACCGAAGGTGCTCAGGCCGCTGACG                                                     <  SRR4302534.2740403/94‑1 (MQ=60)
                                           GCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGT                                                    >  SRR4302534.4373023/1‑94 (MQ=60)
                                           GCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGT                                                    >  SRR4302534.3537356/1‑94 (MQ=60)
                                           GCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGT                                                    >  SRR4302534.2925755/1‑94 (MQ=60)
                                            CGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTT                                                   >  SRR4302534.3383405/1‑94 (MQ=60)
                                            CGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTT                                                   >  SRR4302534.3385283/1‑94 (MQ=60)
                                             GGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTC                                                  >  SRR4302534.4650476/1‑94 (MQ=60)
                                             GGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTC                                                  >  SRR4302534.959559/1‑94 (MQ=60)
                                             GGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTC                                                  >  SRR4302534.6642695/1‑94 (MQ=60)
                                              GCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCC                                                 <  SRR4302534.4638769/94‑1 (MQ=60)
                                               CAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCA                                                <  SRR4302534.1374367/94‑1 (MQ=60)
                                               CAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCA                                                <  SRR4302534.1876297/94‑1 (MQ=60)
                                                AACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCAT                                               >  SRR4302534.2876565/1‑94 (MQ=60)
                                                AACGGAAGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCAT                                               >  SRR4302534.4181372/1‑94 (MQ=60)
                                                 ACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATG                                              >  SRR4302534.310808/1‑94 (MQ=60)
                                                  CGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGC                                             >  SRR4302534.4806953/1‑94 (MQ=60)
                                                  CGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGC                                             >  SRR4302534.869824/1‑94 (MQ=60)
                                                   GGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCC                                            <  SRR4302534.1022698/94‑1 (MQ=60)
                                                   GGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCC                                            >  SRR4302534.39885/1‑94 (MQ=60)
                                                   GGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCC                                            >  SRR4302534.5023326/1‑94 (MQ=60)
                                                   GGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCC                                            >  SRR4302534.1320657/1‑94 (MQ=60)
                                                    GAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCG                                           >  SRR4302534.4457612/1‑94 (MQ=60)
                                                    GAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCG                                           >  SRR4302534.5112883/1‑94 (MQ=60)
                                                    GAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCG                                           <  SRR4302534.2017397/94‑1 (MQ=60)
                                                    GAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCG                                           >  SRR4302534.5325154/1‑94 (MQ=60)
                                                        CACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCAT                                       >  SRR4302534.4435042/1‑94 (MQ=60)
                                                        CACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCAT                                       >  SRR4302534.3974594/1‑94 (MQ=60)
                                                         ACTTTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATA                                      >  SRR4302534.1854951/1‑94 (MQ=60)
                                                            TTCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAG                                   <  SRR4302534.3689326/94‑1 (MQ=60)
                                                             TCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGG                                  >  SRR4302534.2335255/1‑94 (MQ=60)
                                                             TCCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGG                                  >  SRR4302534.3536808/1‑94 (MQ=60)
                                                              CCACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGT                                 <  SRR4302534.2811767/94‑1 (MQ=60)
                                                              CCACACCGATGAACGACCACAGCGTAACGTTGAGGGAACTTTGAATTGCACCGATGGTGACCAGGCCGCTGACGGTCCATGCAGCCATATAGGT                                 >  SRR4302534.2531502/1‑94 (MQ=60)
                                                               CACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTT                                <  SRR4302534.360562/94‑1 (MQ=60)
                                                               CACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTT                                >  SRR4302534.6034230/1‑94 (MQ=60)
                                                               CACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTT                                <  SRR4302534.1410796/94‑1 (MQ=60)
                                                               CACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTT                                >  SRR4302534.4558601/1‑94 (MQ=60)
                                                               CACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTT                                <  SRR4302534.324891/94‑1 (MQ=60)
                                                                ACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTT                               >  SRR4302534.570874/1‑94 (MQ=60)
                                                                ACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTT                               >  SRR4302534.980673/1‑94 (MQ=60)
                                                                ACACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTT                               <  SRR4302534.4777370/94‑1 (MQ=60)
                                                                 CACCGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTC                              >  SRR4302534.3302456/1‑94 (MQ=60)
                                                                  ccgGATGAACGGCCACAGCGTAACGTGTAGGGTACTTGGAATGCCGCCAAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCA                             <  SRR4302534.2272044/91‑1 (MQ=59)
                                                                   CCGATGAACGACCACAGCGTAACGTGGAGGGTACTTTGAATGGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCAC                            <  SRR4302534.4692249/94‑1 (MQ=60)
                                                                    CGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACC                           >  SRR4302534.924938/1‑94 (MQ=60)
                                                                    CGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACC                           <  SRR4302534.2675644/94‑1 (MQ=60)
                                                                    CGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACC                           >  SRR4302534.1590477/1‑94 (MQ=60)
                                                                    CGATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACC                           <  SRR4302534.4132809/94‑1 (MQ=60)
                                                                     GATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCA                          >  SRR4302534.2676939/1‑94 (MQ=60)
                                                                     GATGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCA                          >  SRR4302534.401663/1‑94 (MQ=60)
                                                                       TGAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACG                        >  SRR4302534.4417787/1‑94 (MQ=60)
                                                                        GAACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGGTCCATGCCGCCATATAGGTTTCACCACGG                       >  SRR4302534.2872495/1‑94 (MQ=60)
                                                                          ACGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGGAA                     <  SRR4302534.2092427/94‑1 (MQ=60)
                                                                           CGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGGAAC                    >  SRR4302534.1897230/1‑94 (MQ=60)
                                                                           CGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGGAAC                    <  SRR4302534.3686215/94‑1 (MQ=60)
                                                                           CGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGGAAC                    >  SRR4302534.5515586/1‑94 (MQ=60)
                                                                           CGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGGAAC                    >  SRR4302534.2012796/1‑94 (MQ=60)
                                                                           CGACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGGAAC                    >  SRR4302534.3039017/1‑94 (MQ=60)
                                                                           AGACCACAGCCTAACGTTGAGGGTACTTAGAATTGCACAGAAGGTACCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCTCGGAAC                    <  SRR4302534.4984192/94‑1 (MQ=60)
                                                                            GACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCGTGCCGCCATATAGGTTTCACCACGGAACC                   <  SRR4302534.1250340/94‑1 (MQ=60)
                                                                            GACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGGAACC                   >  SRR4302534.4785293/1‑94 (MQ=60)
                                                                            GACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGGAACC                   >  SRR4302534.1499791/1‑94 (MQ=60)
                                                                            GACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGGAACC                   >  SRR4302534.2889579/1‑94 (MQ=60)
                                                                            GACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGGAACC                   >  SRR4302534.1033085/1‑94 (MQ=60)
                                                                             ACGACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGGAACCA                  >  SRR4302534.6345858/1‑94 (MQ=60)
                                                                             ACCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGGAACCA                  >  SRR4302534.601643/1‑94 (MQ=60)
                                                                              CCACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGGAACCAG                 >  SRR4302534.2120550/1‑94 (MQ=60)
                                                                               CACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGGAACCAGA                >  SRR4302534.5618401/1‑94 (MQ=60)
                                                                                ACAGGGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGTTGACGTTCCATGCCGCCATATAGGTTTCACCACGGAACCAGAA               <  SRR4302534.2309712/94‑1 (MQ=60)
                                                                                ACAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACTGAACCAGAA               <  SRR4302534.1026913/94‑1 (MQ=60)
                                                                                 CAGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGGAACCAGAAC              >  SRR4302534.1224120/1‑94 (MQ=60)
                                                                                  AGCGTAACGTTGAGGGTGCTTTGAATTGCACCGAAGGTGCCAAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGGAACCAGAACC             <  SRR4302534.1141435/94‑1 (MQ=60)
                                                                                  AGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGGAACCAGAACC             <  SRR4302534.1669541/94‑1 (MQ=60)
                                                                                  AGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGGAACCAGAACC             <  SRR4302534.4720836/94‑1 (MQ=60)
                                                                                  AGCGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGGAACCAGAACC             <  SRR4302534.6636864/94‑1 (MQ=60)
                                                                                    CGTAACGTTGGCGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGGAACCAGAACCAG           <  SRR4302534.6555390/94‑1 (MQ=60)
                                                                                    CGTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGGAACCAGAACCAG           >  SRR4302534.5745423/1‑94 (MQ=60)
                                                                                     GTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGGAACCAGAACCAGC          >  SRR4302534.5285834/1‑94 (MQ=60)
                                                                                     GTAACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGGAACCAGAACCAGC          >  SRR4302534.1495850/1‑94 (MQ=60)
                                                                                       AACGTTGAGGGTACTTTGAATTGCACCTAAGGTACCCAGGCCGCTGACGTTCCAGGCCGCCATATAGGTTTCACCACGGAACCAGAACCAGCCA        <  SRR4302534.1728430/94‑1 (MQ=60)
                                                                                       AACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCTAGGCCGATGACGTTCCATGCCGCCATATAGGTTTCACCACGGAACCAGAACCAGCCA        <  SRR4302534.3964481/94‑1 (MQ=60)
                                                                                       AACGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGGAACCAGAACCAGCCA        >  SRR4302534.3571424/1‑94 (MQ=60)
                                                                                         CGTTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGGAACCAGAACCAGCCAAA      >  SRR4302534.6488795/1‑94 (MQ=60)
                                                                                           TTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGGAACCAGAACCAGCCACATA    >  SRR4302534.2327591/1‑94 (MQ=60)
                                                                                           TTGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGGAACCAGAACCAGCCAAATA    <  SRR4302534.3639764/94‑1 (MQ=60)
                                                                                            TGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGGAACCAGAACCAGCCAAATAC   <  SRR4302534.4104446/94‑1 (MQ=60)
                                                                                            TGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGGAACCAGAACCAGCCAAATAC   <  SRR4302534.3323631/94‑1 (MQ=60)
                                                                                            TGAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGGAACCAGAACCAGCCAAATAC   >  SRR4302534.5385397/1‑94 (MQ=60)
                                                                                             GAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGGAACCAGAACCAGCCAAATACG  <  SRR4302534.6400681/94‑1 (MQ=60)
                                                                                             GAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGGAACCAGAACCAGCCAAATACG  >  SRR4302534.1805578/1‑94 (MQ=60)
                                                                                             GAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGGAACCAGAACCAGCCAAATACG  >  SRR4302534.335051/1‑94 (MQ=60)
                                                                                             |                                                                                             
GGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGGAACCAGAACCAGCCAAATACG  >  NC_000913/4333926‑4334112

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑