Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	884	257.2	12958910	96.9%	12557183	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,413,673	C→T	A70A (GCG→GCA)	b4188 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,413,673	0	C	T	100.0%	992.0 / NA	252	A70A (GCG→GCA)	b4188	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (136/116); total (136/116)

Only 100 of 256 total aligned reads displayed.

TGTAGATGACACTCATGCATACAGTATCGCCTGTACGCGCCAGTTATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGACGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCCCCTGAAATATTGCTAACTGAGATCTGACCTATTTC  >  NC_000913/4413583‑4413762

tGTAGATGACACTCATGCATACAGTATCGCCTGTACGCGCCAGTTATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGc                                                                                         <  2:4286460/93‑1 (MQ=255)

  tAGATGACACTCATGCATACAGTATCGCCTGTACGCGCCAGTTATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCAc                                                                                       >  1:2739736/1‑93 (MQ=255)

    gATGACACTCATGCATACAGTATCGCCTGTACGCGCCAGTTATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCt                                                                                     <  1:2813024/93‑1 (MQ=255)

           cTCATGCATACAGTATCGCCTGTACGCGCCAGTTATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCAt                                                                              <  2:5981153/93‑1 (MQ=255)

            tCATGCATACAGTATCGCCTGTACGCGCCAGTTGTCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATc                                                                             <  2:5303827/93‑1 (MQ=255)

             cATGCATACAGTATCGCCTGTACGCGCCAGTTATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCg                                                                            <  2:4244553/93‑1 (MQ=255)

             cATGCATACAGTATCGCCTGTACGCGCCAGTTATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCg                                                                            <  1:5550798/93‑1 (MQ=255)

              aTGCATACAGTATCGCCTGTACGCGCCAGTTATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCgg                                                                           >  1:4191367/1‑93 (MQ=255)

              aTGCATACAGTATCGCCTGTACGCGCCAGTTATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCgg                                                                           <  2:2939196/93‑1 (MQ=255)

              aTGCATACAGTATCGCCTGTACGCGCCAGTTATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCgg                                                                           >  1:3432068/1‑93 (MQ=255)

               tGCATACAGTATCGCCTGTACGCGCCAGTTATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGc                                                                          >  1:3302178/1‑93 (MQ=255)

                gCATACAGTATCGCCTGTACGCGCCAGTTATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGcc                                                                         >  1:4985787/1‑93 (MQ=255)

                 cATACAGTATCGCCTGTACGCGCCAGTTATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCt                                                                        <  1:5273755/93‑1 (MQ=255)

                  aTACAGTATCGCCTGTACGCGCCAGTTATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCtt                                                                       >  1:1333540/1‑93 (MQ=255)

                   tACAGTATCGCCTGTACGCGCCAGTTATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCttt                                                                      >  2:1649401/1‑93 (MQ=255)

                    aCAGTATCGCCTGTACGCGCCAGTTATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTa                                                                     >  2:2091096/1‑93 (MQ=255)

                    aCAGTATCGCCTGTACGCGCCAGTTATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTa                                                                     <  1:1033888/93‑1 (MQ=255)

                      aGTATCGCCTGTACGCGCCAGTTATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAAc                                                                   <  2:2666237/93‑1 (MQ=255)

                      aGTATCGCCTGTACGCGCCAGTTATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAAc                                                                   >  2:3254980/1‑93 (MQ=255)

                       gTATCGCCTGTACGCGCCAGTTATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACg                                                                  >  1:4390859/1‑93 (MQ=255)

                         aTCGCCTGTACGCGCCAGTTATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGcc                                                                >  2:58269/1‑93 (MQ=255)

                          tCGCCTGTACGCGCCAGTTATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCa                                                               <  2:4073893/93‑1 (MQ=255)

                            gCCTGTACGCGCCAGTTATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACg                                                             >  2:4370114/1‑93 (MQ=255)

                            gCCTGTACGCGCCAGTTATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACg                                                             >  2:479880/1‑93 (MQ=255)

                            gCCTGTACGCGCCAGTTATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACg                                                             >  1:768955/1‑93 (MQ=255)

                            gCCTGTACGCGCCAGTTATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACg                                                             >  2:917772/1‑93 (MQ=255)

                            gCCTGTACGCGCCAGTTATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACg                                                             >  2:5233554/1‑93 (MQ=255)

                              cTGTACGCGCCAGTTATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGAt                                                           >  1:274977/1‑93 (MQ=255)

                               tGTACGCGCCAGTTATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATa                                                          >  1:159996/1‑93 (MQ=255)

                               tGTACGCGCCAGTTATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATa                                                          >  1:3885373/1‑93 (MQ=255)

                                 tACGCGCCAGTTATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACg                                                        <  2:6047323/93‑1 (MQ=255)

                                   cgcgCCAGTTATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGtt                                                      <  2:6287920/93‑1 (MQ=255)

                                     cgcCAGTTATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTcc                                                    <  1:5801656/93‑1 (MQ=255)

                                      gcCAGTTATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCa                                                   >  2:5468077/1‑93 (MQ=255)

                                       ccAGTTATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTTTTCATCGGCCTTTAACGCCACGATACGTTCCAc                                                  <  1:4524256/93‑1 (MQ=255)

                                        cAGTTATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACg                                                 >  2:690126/1‑93 (MQ=255)

                                        cAGTTATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACg                                                 <  2:5023088/93‑1 (MQ=255)

                                         aGTTATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGt                                                >  2:4879760/1‑93 (MQ=255)

                                         aGTTATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGt                                                >  2:2323440/1‑93 (MQ=255)

                                         aGTTATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCAGGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGt                                                <  1:2462073/93‑1 (MQ=255)

                                          gTTATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTc                                               <  2:4410488/93‑1 (MQ=255)

                                          gTTATAAGCCTCCTGGTCTTCGTACATGGTAATAAGGCGGTCCCATGAGGCCCCTTGTTCACCGGCC‑TTAACGCCACGATACGTTCCACGTcc                                              <  2:5070329/93‑1 (MQ=255)

                                           ttATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTcc                                              <  1:265429/93‑1 (MQ=255)

                                             aTCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTg                                            >  2:2499954/1‑93 (MQ=255)

                                             aTCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTg                                            >  2:1071583/1‑93 (MQ=255)

                                              tCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGc                                           <  2:1748705/93‑1 (MQ=255)

                                               cAGGCTGCTGGTCTTCGTACATGTTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCg                                          <  1:494035/93‑1 (MQ=255)

                                               cAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCg                                          <  2:3566087/93‑1 (MQ=255)

                                                aGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCgg                                         >  2:1487351/1‑93 (MQ=255)

                                                aGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCgg                                         >  1:4827197/1‑93 (MQ=255)

                                                 ggCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACTCCACGATACGTTCCACGTCCTGCGGa                                        >  1:2335133/1‑93 (MQ=255)

                                                  actgctgGTCTTCGTACATGGTAATAATGCGGTACCATGATGCATCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGAt                                       <  1:1986648/92‑1 (MQ=255)

                                                   ctgctgGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATc                                      >  1:625349/1‑93 (MQ=255)

                                                   ctgctgGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATc                                      <  1:4730897/93‑1 (MQ=255)

                                                    tgctgGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATcc                                     >  2:238021/1‑93 (MQ=255)

                                                    tgctgGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATcc                                     >  1:3205476/1‑93 (MQ=255)

                                                    tgctgGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATcc                                     >  2:2558/1‑93 (MQ=255)

                                                    tgctgGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATcc                                     >  2:4005519/1‑93 (MQ=255)

                                                      ctgGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATcccc                                   <  1:5384506/93‑1 (MQ=255)

                                                       tgGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCCCCt                                  <  1:3406614/93‑1 (MQ=255)

                                                       tgGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCCCCt                                  <  2:336670/93‑1 (MQ=255)

                                                       tgGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCCCCt                                  <  2:2417682/93‑1 (MQ=255)

                                                       tgGTCTTCGTACATCGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCCCCt                                  <  2:4399362/93‑1 (MQ=255)

                                                        ggTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCCCCTg                                 >  1:3795782/1‑93 (MQ=255)

                                                        ggTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCCCCTg                                 <  1:2407291/93‑1 (MQ=255)

                                                        ggTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCCCCTg                                 >  2:3960195/1‑93 (MQ=255)

                                                         gTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCCCCTGa                                >  1:3549664/1‑93 (MQ=255)

                                                            ttCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCCCCTGAAat                             >  2:4182716/1‑93 (MQ=255)

                                                             tCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCCCCTGAAata                            >  1:1673547/1‑93 (MQ=255)

                                                              cGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCCCCTGAAatat                           <  1:6389448/93‑1 (MQ=255)

                                                              cGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCCCCTGAAatat                           >  2:5653220/1‑93 (MQ=255)

                                                               gTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCCCCTGAAATAtt                          <  2:4432954/93‑1 (MQ=255)

                                                                 aCATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCCCCTGAAATATTGc                        >  2:621866/1‑93 (MQ=255)

                                                                 aCATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCCCCTGAAATATTGc                        <  1:6203484/93‑1 (MQ=255)

                                                                 aCATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCCCCTGAAATATTGc                        >  1:5746986/1‑93 (MQ=255)

                                                                 aCATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCCCCTGAAATATTGc                        >  2:1323603/1‑93 (MQ=255)

                                                                 aCATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCCCCTGAAATATTGc                        >  2:2284181/1‑93 (MQ=255)

                                                                 aCATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCCCCTGAAATATTGc                        >  1:5910760/1‑93 (MQ=255)

                                                                    aggTAATAATGCGGTACCATGATGCACCTGGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCCCCTGAAATATTGCTaa                     <  1:3991742/92‑1 (MQ=255)

                                                                     ggTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCCCCTGAAATATTGCTAAc                    <  2:4580610/93‑1 (MQ=255)

                                                                      gTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCCCCTGAAATATTGCTAACt                   <  1:446245/93‑1 (MQ=255)

                                                                       taacaaTGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCCCCTGAAATATTGCTAACTg                  <  2:3043130/93‑1 (MQ=255)

                                                                        aataatGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCCCCTGAAATATTGCTAACTga                 >  2:3772386/1‑93 (MQ=255)

                                                                        aataatGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCCCCTGAAATATTGCTAACTga                 >  1:595089/1‑93 (MQ=255)

                                                                         ataatGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCCCCTGAAATATTGCTAACTgag                >  1:4552744/1‑93 (MQ=255)

                                                                         ataatGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCCCCTGAAATATTGCTAACTgag                >  2:742819/1‑93 (MQ=255)

                                                                          taatGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCCCCTGAAATATTGCTAACTgaga               >  2:2159639/1‑93 (MQ=255)

                                                                          taatGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCCCCTGAAATATTGCTAACTgaga               <  2:1007196/93‑1 (MQ=255)

                                                                           aatGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCCCCTGAAATATTGCTAACTGAGAt              >  1:283821/1‑93 (MQ=255)

                                                                             tGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCCCCTGAAATATTGCTAACTGAGATCt            <  1:4287717/93‑1 (MQ=255)

                                                                             tGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCCCCTGAAATATTGCTAACTGAGATCt            <  2:1268239/93‑1 (MQ=255)

                                                                                  tACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCCCCTGAAATATTGCTAACTGAGATCTGACCt       >  1:2135525/1‑93 (MQ=255)

                                                                                  tACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCCCCTGAAATATTGCTAACTGAGATCTGACCt       >  1:1092732/1‑93 (MQ=255)

                                                                                   aCCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCCCCTGAAATATTGCTAACTGAGATCTGACCTa      <  1:3074528/93‑1 (MQ=255)

                                                                                   aCCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCCCCTGAAATATTGCTAACTGAGATCTGACCTa      >  1:1216652/1‑93 (MQ=255)

                                                                                    ccATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCCCCTGAAATATTGCTAACTGAGATCTGACCTAt     >  1:1522473/1‑93 (MQ=255)

                                                                                     cATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCCCCTGAAATATTGCTAACTGAGATCTGACCTAtt    <  1:6419930/93‑1 (MQ=255)

                                                                                      atgatgCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCCCCTGAAATATTGCTAACTGAGATCTGACCTAttt   <  2:42982/93‑1 (MQ=255)

                                                                                      atgatgCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCCCCTGAAATATTGCTAACTGAGATCTGACCTAttt   >  2:3798661/1‑93 (MQ=255)

                                                                                       tGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCCCCTGAAATATTGCTAACTGAGATCTGACCTATttc  >  1:6084503/1‑93 (MQ=255)

TGTAGATGACACTCATGCATACAGTATCGCCTGTACGCGCCAGTTATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGACGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCCCCTGAAATATTGCTAACTGAGATCTGACCTATTTC  >  NC_000913/4413583‑4413762

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GCTGTAGATGACACTCATGCATACAGTATCGCCTGTACGCGCCAGTTATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGACGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCCCCTGAAATATTGCTAACTGAGATCTGACCTATTTCATTC  >  NC_000913/4413581‑4413766

GCTGTAGATGACACTCATGCATACAGTATCGCCTGTACGCGCCAGTTATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATG                                                                                              <  SRR4302534.5763685/94‑1 (MQ=60)

 CTGTAGATGACACTCATGCATACAGTATCGCCTGTACGCGCCAGTTATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGC                                                                                             <  SRR4302534.3913416/94‑1 (MQ=60)

 CTGTAGATGACACTCATGCATACAGTATCGCCTGTACGCGCCAGTTATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGC                                                                                             <  SRR4302534.4943441/94‑1 (MQ=60)

  TGTAGATGACACTCATGCATACAGTATCGCCTGTACGCGCCAGTTATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCA                                                                                            <  SRR4302534.1164288/94‑1 (MQ=60)

   GTAGATGACACTCATGCATACAGTATCGCCTGTACGCGCCAGTTATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCAC                                                                                           >  SRR4302534.2811928/1‑94 (MQ=60)

     AGATGACACTCATGCATACAGTATCGCCTGTACGCGCCAGTTATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCT                                                                                         >  SRR4302534.6099294/1‑94 (MQ=60)

      GATGACACTCATGCATACAGTATCGCCTGTACGCGCCAGTTATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTT                                                                                        <  SRR4302534.2886977/94‑1 (MQ=60)

      GATGACACTCATGCATACAGTATCGCCTGTACGCGCCAGTTATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTT                                                                                        <  SRR4302534.2857056/94‑1 (MQ=60)

       ATGACACTCATGCATACAGTATCGCCTGTACGCGCCAGTTATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTG                                                                                       <  SRR4302534.5767214/94‑1 (MQ=60)

        TGACACTCATGCATACAGTATCGCCTGTACGCGCCAGTTATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGT                                                                                      >  SRR4302534.5929519/1‑94 (MQ=60)

            ACTCATGCATACAGTATCGCCTGTACGCGCCAGTTATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCAT                                                                                  <  SRR4302534.115883/94‑1 (MQ=60)

            ACTCATGCATACAGTATCGCCTGTACGCGCCAGTTATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCAT                                                                                  >  SRR4302534.212580/1‑94 (MQ=60)

            ACTCATGCATACAGTATCGCCTGTACGCGCCAGTTATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCAT                                                                                  >  SRR4302534.6491724/1‑94 (MQ=60)

             CTCATGCATACAGTATCGCCTGTACGCGCCAGTTATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATC                                                                                 <  SRR4302534.4623177/94‑1 (MQ=60)

              ccatgcaaccaggATCGCCCGTACGCGCCAGTTTGCCGGCTGCTGGTCTTCGTACATGGTAATAATGGGGTTCCATGATGCACCTTGTTCATCG                                                                                <  SRR4302534.3166821/81‑1 (MQ=53)

              TCATGCATACAGTATCGCCTGTACGCGCCAGTTATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCG                                                                                >  SRR4302534.1701348/1‑94 (MQ=60)

              TCATGCATACAGTATCGCCTGTACGCGCCAGTTATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCG                                                                                >  SRR4302534.4072633/1‑94 (MQ=60)

               CATGCATACAGTATCGCCTGTACGCGCCAGTTATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGG                                                                               >  SRR4302534.4304602/1‑94 (MQ=60)

               CATGCATACAGTATCGCCTGTACGCGCCAGTTATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGG                                                                               <  SRR4302534.5700648/94‑1 (MQ=60)

               CATGCATACAGTATCGCCTGTACGCGCCAGTTATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGG                                                                               >  SRR4302534.3524287/1‑94 (MQ=60)

                ATGCATACAGTATCGCCTGTACGCGCCAGTTATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGC                                                                              >  SRR4302534.3390324/1‑94 (MQ=60)

                 TGCATACAGTATCGCCTGTACGCGCCAGTTATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCC                                                                             >  SRR4302534.5120708/1‑94 (MQ=60)

                 TGCATACAGTATCGCCTGTACGCGCCAGTTATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCC                                                                             <  SRR4302534.3397517/94‑1 (MQ=60)

                  GCATACAGTATCGCCTGTACGCGCCAGTTATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCT                                                                            <  SRR4302534.1698738/94‑1 (MQ=60)

                  GCATACAGTATCGCCTGTACGCGCCAGTTATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCT                                                                            >  SRR4302534.2523074/1‑94 (MQ=60)

                   CATACAGTATCGCCTGTACGCGCCAGTTATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTT                                                                           <  SRR4302534.5416192/94‑1 (MQ=60)

                   CATACAGTATCGCCTGTACGCGCCAGTTATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTT                                                                           <  SRR4302534.2675097/94‑1 (MQ=60)

                   CATACAGTATCGCCTGTACGCGCCAGTTATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTT                                                                           >  SRR4302534.3548169/1‑94 (MQ=60)

                   CATACAGTATCGCCTGTACGCGCCAGTTATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTT                                                                           >  SRR4302534.1368691/1‑94 (MQ=60)

                    ATACAGTATCGCCTGTACGCGCCAGTTATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTT                                                                          >  SRR4302534.4954467/1‑94 (MQ=60)

                    ATACAGTATCGCCTGTACGCGCCAGTTATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTT                                                                          >  SRR4302534.3372825/1‑94 (MQ=60)

                    ATACAGTATCGCCTGTACGCGCCAGTTATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTT                                                                          >  SRR4302534.4648919/1‑94 (MQ=60)

                     TACAGTATCGCCTGTACGCGCCAGTTATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTA                                                                         <  SRR4302534.3052174/94‑1 (MQ=60)

                     TACAGTATCGCCTGTACGCGCCAGTTATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTA                                                                         <  SRR4302534.6390390/94‑1 (MQ=60)

                      ACAGTATCGCCTGTACGCGCCAGTTATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAA                                                                        >  SRR4302534.5696566/1‑94 (MQ=60)

                      ACAGTATCGCCTGTACGCGCCAGTTATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAA                                                                        <  SRR4302534.1060562/94‑1 (MQ=60)

                        AGTATCGCCTGTACGCGCCAGTTATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACG                                                                      >  SRR4302534.4509275/1‑94 (MQ=60)

                          TATCGCGTGTACGCGCCAGTTATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTCCCATGATGCACCTTGTTCATCGGCCTTTAACGCC                                                                    <  SRR4302534.3829880/94‑1 (MQ=60)

                          TATCGCCTGTACGCGCCAGTTATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCC                                                                    <  SRR4302534.4920251/94‑1 (MQ=60)

                          TATCGCCTGTACGCGCCAGTTATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCC                                                                    >  SRR4302534.1609288/1‑94 (MQ=60)

                           ATCGCCTGTACGCGCCAGTTATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCA                                                                   >  SRR4302534.4183672/1‑94 (MQ=60)

                           ATCGCCTGTACGCGCCAGTTATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCA                                                                   >  SRR4302534.65700/1‑94 (MQ=60)

                             CGCCTGTACGCGCCAGTTATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTCCCATGATGCACCTTGTTCATCGGCCTTTAACGCCACG                                                                 <  SRR4302534.5941119/94‑1 (MQ=60)

                             CGCCTGTACGCGCCAGTTATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACG                                                                 <  SRR4302534.1257907/94‑1 (MQ=60)

                             CGCCTGTACGCGCCAGTTATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACG                                                                 >  SRR4302534.788076/1‑94 (MQ=60)

                               CCTGTACGCGCCAGTTATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGAT                                                               <  SRR4302534.2401520/94‑1 (MQ=60)

                               CCTGTACGCGCCAGTTATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGAT                                                               >  SRR4302534.282093/1‑94 (MQ=60)

                               CCTGTACGCGCCAGTTATCA‑GCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATA                                                              >  SRR4302534.4542154/1‑94 (MQ=60)

                                CTGTACGCGCCAGTTATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATA                                                              >  SRR4302534.3989680/1‑94 (MQ=60)

                                CTGTACGCGCCAGTTATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATA                                                              >  SRR4302534.164184/1‑94 (MQ=60)

                                  GTACGCGCCAGTTATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACG                                                            <  SRR4302534.986489/94‑1 (MQ=60)

                                   TACGCGCCAGTTATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGT                                                           >  SRR4302534.2892332/1‑94 (MQ=60)

                                   TACGCGCCAGTTATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGT                                                           >  SRR4302534.899769/1‑94 (MQ=60)

                                     CGCGCCAGTTATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTC                                                         <  SRR4302534.4004606/94‑1 (MQ=60)

                                       CGCCAGTTATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCA                                                       <  SRR4302534.5959097/94‑1 (MQ=60)

                                        GCCAGTTATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCAC                                                      <  SRR4302534.6585757/94‑1 (MQ=60)

                                         CCAGTTATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTTTTCATCGGCCTTTAACGCCACGATACGTTCCACG                                                     <  SRR4302534.4646812/94‑1 (MQ=60)

                                         CCAGTTATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACG                                                     >  SRR4302534.2859575/1‑94 (MQ=60)

                                          CAGTTATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGT                                                    >  SRR4302534.6232323/1‑94 (MQ=60)

                                           AGTTATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTC                                                   >  SRR4302534.6651630/1‑94 (MQ=60)

                                           AGTTATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCAGGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTC                                                   <  SRR4302534.2527481/94‑1 (MQ=60)

                                             TTATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCT                                                 <  SRR4302534.272316/94‑1 (MQ=60)

                                              TATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTG                                                <  SRR4302534.3460569/94‑1 (MQ=60)

                                              TATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTG                                                >  SRR4302534.3918377/1‑94 (MQ=60)

                                              TATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTG                                                <  SRR4302534.6187284/94‑1 (MQ=60)

                                                 CAGGCTGCTGGTCTTCGTACATGTTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGG                                             <  SRR4302534.506359/94‑1 (MQ=60)

                                                 CAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGG                                             >  SRR4302534.3120549/1‑94 (MQ=60)

                                                 CAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGG                                             >  SRR4302534.1789475/1‑94 (MQ=60)

                                                 CAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGG                                             >  SRR4302534.4958182/1‑94 (MQ=60)

                                                  AGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACTCCACGATACGTTCCACGTCCTGCGGA                                            >  SRR4302534.2397077/1‑94 (MQ=60)

                                                   GGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGAT                                           <  SRR4302534.5403408/94‑1 (MQ=60)

                                                    GCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATC                                          >  SRR4302534.4388336/1‑94 (MQ=60)

                                                    GCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATC                                          >  SRR4302534.640516/1‑94 (MQ=60)

                                                    GCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATC                                          <  SRR4302534.4712130/94‑1 (MQ=60)

                                                    ACTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCATCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATC                                          <  SRR4302534.2038668/94‑1 (MQ=60)

                                                     CTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCC                                         >  SRR4302534.3263912/1‑94 (MQ=60)

                                                     CTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCC                                         >  SRR4302534.3290481/1‑94 (MQ=60)

                                                     CTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCC                                         <  SRR4302534.4859365/94‑1 (MQ=60)

                                                      TGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCCC                                        <  SRR4302534.2815428/94‑1 (MQ=60)

                                                       GCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCCCC                                       <  SRR4302534.4533363/94‑1 (MQ=60)

                                                       GCTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCCCC                                       >  SRR4302534.5063623/1‑94 (MQ=60)

                                                        CTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCCCCT                                      <  SRR4302534.5529540/94‑1 (MQ=60)

                                                        CTGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCCCCT                                      >  SRR4302534.5067244/1‑94 (MQ=60)

                                                         TGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCCCCTG                                     <  SRR4302534.6333085/94‑1 (MQ=60)

                                                         TGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCCCCTG                                     >  SRR4302534.3897696/1‑94 (MQ=60)

                                                         TGGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCCCCTG                                     <  SRR4302534.3498100/94‑1 (MQ=60)

                                                          GGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCCCCTGA                                    >  SRR4302534.6387231/1‑94 (MQ=60)

                                                          GGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCCCCTGA                                    <  SRR4302534.2471270/94‑1 (MQ=60)

                                                          GGTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCCCCTGA                                    >  SRR4302534.3645115/1‑94 (MQ=60)

                                                          GGTCTTCGTACATGCTAATAAGACGTTATCATGAGGCACCTGGTTCATCGGCCTTTAACGCCACGATACTTTCCACGTCCTGCGGATCCCCTGA                                    <  SRR4302534.6205842/94‑1 (MQ=60)

                                                           GTCTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCCCCTGAA                                   >  SRR4302534.152622/1‑94 (MQ=60)

                                                             CTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCCCCTGAAAT                                 >  SRR4302534.1082934/1‑94 (MQ=60)

                                                             CTTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCCCCTGAAAT                                 <  SRR4302534.6081917/94‑1 (MQ=60)

                                                              TTCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCCCCTGAAATA                                >  SRR4302534.1717091/1‑94 (MQ=60)

                                                               TCGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCCCCTGAAATAT                               >  SRR4302534.2797558/1‑94 (MQ=60)

                                                                CGTACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCCCCTGAAATATT                              <  SRR4302534.6562024/94‑1 (MQ=60)

                                                                  TACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCCCCTGAAATATTGC                            >  SRR4302534.6071264/1‑94 (MQ=60)

                                                                  TACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCCCCTGAAATATTGC                            >  SRR4302534.5902810/1‑94 (MQ=60)

                                                                   ACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCCCCTGAAATATTGCT                           <  SRR4302534.1412352/94‑1 (MQ=60)

                                                                   ACATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCCCCTGAAATATTGCT                           <  SRR4302534.6372179/94‑1 (MQ=60)

                                                                    CATGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCCCCTGAAATATTGCTA                          >  SRR4302534.2079423/1‑94 (MQ=60)

                                                                      TGGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCCCCTGAAATATTGCTAAC                        >  SRR4302534.4468669/1‑94 (MQ=60)

                                                                      AGGTAATAATGCGGTACCATGATGCACCTGGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCCCCTGAAATATTGCTAAC                        <  SRR4302534.4098589/94‑1 (MQ=60)

                                                                       GGTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCCCCTGAAATATTGCTAACT                       >  SRR4302534.6036119/1‑94 (MQ=60)

                                                                        GTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCCCCTGAAATATTGCTAACTG                      <  SRR4302534.457562/94‑1 (MQ=60)

                                                                        GTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCCCCTGAAATATTGCTAACTG                      >  SRR4302534.396517/1‑94 (MQ=60)

                                                                        GTAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCCCCTGAAATATTGCTAACTG                      <  SRR4302534.2275147/94‑1 (MQ=60)

                                                                         TAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCCCCTGAAATATTGCTAACTGA                     >  SRR4302534.609614/1‑94 (MQ=60)

                                                                         TAATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCCCCTGAAATATTGCTAACTGA                     <  SRR4302534.4657686/94‑1 (MQ=60)

                                                                          AATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCCCCTGAAATATTGCTAACTGAG                    >  SRR4302534.4676159/1‑94 (MQ=60)

                                                                          AATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCCCCTGAAATATTGCTAACTGAG                    <  SRR4302534.5433950/94‑1 (MQ=60)

                                                                           ATAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCCCCTGAAATATTGCTAACTGAGA                   >  SRR4302534.4386842/1‑94 (MQ=60)

                                                                            TAATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCCCCTGAAATATTGCTAACTGAGAT                  >  SRR4302534.291154/1‑94 (MQ=60)

                                                                             AATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCCCCTGAAATATTGCTAACTGAGATC                 <  SRR4302534.5076708/94‑1 (MQ=60)

                                                                              ATGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCCCCTGAAATATTGCTAACTGAGATCT                <  SRR4302534.3505990/94‑1 (MQ=60)

                                                                               TGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCGCGTCCTGCGGATCCCCTGAAATATTGCTAACTGAGATCTG               <  SRR4302534.5662059/94‑1 (MQ=60)

                                                                               TGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCCCCTGAAATATTGCTAACTGAGATCTG               >  SRR4302534.2773600/1‑94 (MQ=60)

                                                                               TGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCCCCTGAAATATTGCTAACTGAGATCTG               <  SRR4302534.4403877/94‑1 (MQ=60)

                                                                               TGCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCCCCTGAAATATTGCTAACTGAGATCTG               <  SRR4302534.2245555/94‑1 (MQ=60)

                                                                                GCGGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCCCCTGAAATATTGCTAACTGAGATCTGA              >  SRR4302534.5220172/1‑94 (MQ=60)

                                                                                  GGTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCCCCTGAAATATTGCTAACTGAGATCTGACC            >  SRR4302534.35792/1‑94 (MQ=60)

                                                                                   GTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCCCCTGAAATATTGCTAACTGAGATCTGACCT           >  SRR4302534.2191825/1‑94 (MQ=60)

                                                                                   GTACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCCCCTGAAATATTGCTAACTGAGATCTGACCT           >  SRR4302534.1121158/1‑94 (MQ=60)

                                                                                    TACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCCCCTGAAATATTGCTAACTGAGATCTGACCTA          >  SRR4302534.1248673/1‑94 (MQ=60)

                                                                                     ACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCCCCTGAAATATTGCTAACTGAGATCTGACCTAT         >  SRR4302534.2479372/1‑94 (MQ=60)

                                                                                     ACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCCCCTGAAATATTGCTAACTGAGATCTGACCTAT         >  SRR4302534.1562442/1‑94 (MQ=60)

                                                                                     ACCATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCCCCTGAAATATTGCTAACTGAGATCTGACCTAT         <  SRR4302534.3155290/94‑1 (MQ=60)

                                                                                       CATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCCCCTGAAATATTGCTAACTGAGATCTGACCTATTT       <  SRR4302534.6593100/94‑1 (MQ=60)

                                                                                        ATGATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCCCCTGAAATATTGCTAACTGAGATCTGACCTATTTC      >  SRR4302534.6249954/1‑94 (MQ=60)

                                                                                           ATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCCCCTGCAATAATTCTAAATGCGATCTGACCTCTTTCATT   >  SRR4302534.1147233/1‑94 (MQ=60)

                                                                                           ATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCCCCTGAAATATTGCTAACTGAGATCTGACCTATTTCATT   >  SRR4302534.6286498/1‑94 (MQ=60)

                                                                                           ATGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCCCCTGAAATATTGCTAACTGAGATCTGACCTATTTCATT   >  SRR4302534.1229354/1‑94 (MQ=60)

                                                                                            TGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCCCCTGAAATATTGCTAACTGAGATCTGACCTATTTCATTC  <  SRR4302534.1688457/94‑1 (MQ=60)

GCTGTAGATGACACTCATGCATACAGTATCGCCTGTACGCGCCAGTTATCAGGCTGCTGGTCTTCGTACATGGTAATAATGCGGTACCATGACGCACCTTGTTCATCGGCCTTTAACGCCACGATACGTTCCACGTCCTGCGGATCCCCTGAAATATTGCTAACTGAGATCTGACCTATTTCATTC  >  NC_000913/4413581‑4413766

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 8 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`