Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 884 257.2 12958910 96.9% 12557183 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,435,684 +A coding (400/501 nt) ytfI → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,435,6811.A99.5% 752.1 / ‑3.5 190coding (397/501 nt)ytfIhypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (1/0);  new base A (98/91);  total (99/91)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 208 total aligned reads displayed.

GAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAAC‑AAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGAT  >  NC_000913/4435589‑4435768
                                                                                             |                                                                                       
gAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGaac                                                                                          >  1:2386326/1‑93 (MQ=255)
 aGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGaac‑a                                                                                        >  1:1711006/1‑93 (MQ=255)
  ggTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGaac‑aa                                                                                       <  2:5105146/93‑1 (MQ=255)
  ggTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGaac‑aa                                                                                       >  2:4140603/1‑93 (MQ=255)
  ggTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGaac‑aa                                                                                       <  1:4311601/93‑1 (MQ=255)
   gTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGaac‑aaa                                                                                      <  2:5722624/93‑1 (MQ=255)
      gTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGaac‑aaaaca                                                                                   >  2:6420562/1‑90 (MQ=255)
       tGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGaac‑aaaacag                                                                                  >  2:4342391/1‑89 (MQ=255)
         gATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGAc                                                                                 >  2:1696809/1‑93 (MQ=255)
          aTTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACg                                                                                >  1:2156851/1‑93 (MQ=255)
           tttGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGa                                                                               >  2:454908/1‑93 (MQ=255)
            ttGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGAt                                                                              <  2:1157860/93‑1 (MQ=255)
                aTACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTa                                                                          <  1:3124045/93‑1 (MQ=255)
                aTACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTa                                                                          <  2:3631557/93‑1 (MQ=255)
                aTACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTa                                                                          <  1:2909038/93‑1 (MQ=255)
                 tACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTAt                                                                         <  2:4877957/93‑1 (MQ=255)
                 tACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTAt                                                                         >  2:3020647/1‑93 (MQ=255)
                   cGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGc                                                                       >  1:3768885/1‑93 (MQ=255)
                   cGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGc                                                                       <  2:1905146/93‑1 (MQ=255)
                    gaagaaATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGcc                                                                      <  1:6222847/93‑1 (MQ=255)
                    gaagaaATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGcc                                                                      >  2:4626651/1‑93 (MQ=255)
                     aagaaATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCt                                                                     <  1:1976591/93‑1 (MQ=255)
                     aagaaATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCt                                                                     >  1:4409768/1‑93 (MQ=255)
                      agaaATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTg                                                                    >  1:3073025/1‑93 (MQ=255)
                      agaaATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTg                                                                    <  2:5363665/93‑1 (MQ=255)
                       gaaATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTgg                                                                   >  2:909120/1‑93 (MQ=255)
                        aaaTACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTggg                                                                  <  2:2319646/93‑1 (MQ=255)
                         aaTACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGa                                                                 >  1:1403789/1‑93 (MQ=255)
                         aaTACCAATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGa                                                                 <  1:5850965/93‑1 (MQ=255)
                          aTACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGat                                                                >  1:2682758/1‑93 (MQ=255)
                          aTACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGat                                                                >  1:4208114/1‑93 (MQ=255)
                          aTACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGat                                                                <  1:1614710/93‑1 (MQ=255)
                           tACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGata                                                               <  1:1124460/93‑1 (MQ=255)
                            aCCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGatat                                                              >  1:6473986/1‑93 (MQ=255)
                             cccATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATAtt                                                             >  2:2772711/1‑93 (MQ=255)
                             cccATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACAATTGTATGCCGGGGATAtt                                                             <  1:6309807/93‑1 (MQ=255)
                              ccATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTc                                                            <  1:6002520/93‑1 (MQ=255)
                              ccATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTc                                                            <  2:4962007/93‑1 (MQ=255)
                               cATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCt                                                           >  2:3487124/1‑93 (MQ=255)
                               cATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCt                                                           <  1:3990296/93‑1 (MQ=255)
                                aTAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACATACGATTGTATGCCTGGGATATTCTa                                                          <  2:1292995/93‑1 (MQ=255)
                                aTAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTa                                                          >  2:4742095/1‑93 (MQ=255)
                                 tAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTAc                                                         >  2:659546/1‑93 (MQ=255)
                                 tAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTAc                                                         >  1:1532362/1‑93 (MQ=255)
                                  aaCAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACa                                                        <  1:4969496/93‑1 (MQ=255)
                                   aCAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAg                                                       <  2:2626110/93‑1 (MQ=255)
                                     aaTAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGaa                                                     >  2:2076386/1‑93 (MQ=255)
                                      aTAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGAATGCCTGGGATATTCTACAGaaa                                                    >  1:1223404/1‑93 (MQ=255)
                                       tAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGaaaa                                                   >  2:5417022/1‑93 (MQ=255)
                                         aaTGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGaaaaaa                                                 >  2:3773350/1‑93 (MQ=255)
                                         aaTGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGaaaaaa                                                 >  1:4842268/1‑93 (MQ=255)
                                          aTGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAAc                                                >  1:5340257/1‑93 (MQ=255)
                                           tGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACa                                               >  2:857730/1‑93 (MQ=255)
                                           agAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACa                                               <  2:691591/92‑1 (MQ=255)
                                            gAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAg                                              >  1:4916331/1‑93 (MQ=255)
                                             aaTATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGt                                             >  2:1437190/1‑93 (MQ=255)
                                               tataACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCt                                           <  2:5225337/93‑1 (MQ=255)
                                               tataACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCt                                           >  2:1786187/1‑93 (MQ=255)
                                               tataACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCt                                           <  1:579791/93‑1 (MQ=255)
                                                ataACTCATTCATGGATTTCAAAACCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTg                                          <  2:6265267/93‑1 (MQ=255)
                                                ataACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTg                                          <  2:5732630/93‑1 (MQ=255)
                                                ataACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTg                                          >  1:1817463/1‑93 (MQ=255)
                                                  aaCTCATTCCTGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGAc                                        >  1:41156/1‑93 (MQ=255)
                                                   aCTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACa                                       >  1:5699932/1‑93 (MQ=255)
                                                    cTCATTCATGGATTTCAAAACCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACAt                                      <  1:2582217/93‑1 (MQ=255)
                                                    cTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACAt                                      >  2:2002941/1‑93 (MQ=255)
                                                     tcattcatGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATa                                     >  1:2963828/1‑93 (MQ=255)
                                                     tcattcatGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATa                                     >  1:4022321/1‑93 (MQ=255)
                                                       attcatGGATTTCAAAAACCGTGAATTTCTGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATAcc                                   <  1:6352690/93‑1 (MQ=255)
                                                       attcatGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATAcc                                   <  2:4287803/93‑1 (MQ=255)
                                                       attcatGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATAcc                                   >  2:3250954/1‑93 (MQ=255)
                                                       attcatGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATAcc                                   <  1:5465482/93‑1 (MQ=255)
                                                        ttcatGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCg                                  >  2:1016995/1‑93 (MQ=255)
                                                        ttcatGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCg                                  >  2:3349937/1‑93 (MQ=255)
                                                           atGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTGCAGAAAAAACAGTCTGACATACCGaaa                               >  2:3124528/1‑93 (MQ=255)
                                                            tGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGaaaa                              <  2:2694441/93‑1 (MQ=255)
                                                            tGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGaaaa                              <  2:2501733/93‑1 (MQ=255)
                                                             ggATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAg                             >  1:3225532/1‑93 (MQ=255)
                                                              gATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAgg                            <  2:2882381/93‑1 (MQ=255)
                                                              gATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAgg                            <  2:5761025/93‑1 (MQ=255)
                                                                     aaaaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAg                     <  2:1711006/93‑1 (MQ=255)
                                                                     aaaaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAg                     >  1:2475645/1‑93 (MQ=255)
                                                                       aaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGt                   <  2:1550523/93‑1 (MQ=255)
                                                                        aCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGtt                  >  1:796444/1‑93 (MQ=255)
                                                                        aCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGtt                  >  1:5749646/1‑93 (MQ=255)
                                                                         ccGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTta                 >  2:4167812/1‑93 (MQ=255)
                                                                         ccGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTta                 <  1:3581490/93‑1 (MQ=255)
                                                                           gTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTtata               >  1:3780473/1‑93 (MQ=255)
                                                                           gTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTtata               <  1:2801762/93‑1 (MQ=255)
                                                                            tGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTatat              <  1:1717960/93‑1 (MQ=255)
                                                                              aaTTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTc            <  1:4799842/93‑1 (MQ=255)
                                                                               aTTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCa           <  2:4405196/93‑1 (MQ=255)
                                                                               aTTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCa           >  1:2271578/1‑93 (MQ=255)
                                                                                tttCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCAc          >  2:4108438/1‑93 (MQ=255)
                                                                                  tCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCa        >  1:2562849/1‑93 (MQ=255)
                                                                                   ccGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAg       >  2:196773/1‑93 (MQ=255)
                                                                                      ttAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCg    <  1:3116725/93‑1 (MQ=255)
                                                                                      ttAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCg    >  1:2788062/1‑93 (MQ=255)
                                                                                      ttAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCg    >  1:1157146/1‑93 (MQ=255)
                                                                                       tAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGa   >  2:705338/1‑93 (MQ=255)
                                                                                             |                                                                                       
GAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAAC‑AAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGAT  >  NC_000913/4435589‑4435768

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAAC‑A‑AACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACG  >  NC_000913/4435591‑4435776
                                                                                               |                                                                                            
GGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAAC‑A‑AA                                                                                              <  SRR4302534.4428411/94‑1 (MQ=60)
   AGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAAC‑A‑AAaca                                                                                           <  SRR4302534.2104567/94‑4 (MQ=60)
   AGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAAC‑A‑AAaca                                                                                           >  SRR4302534.5057983/1‑91 (MQ=60)
    GTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AACAG                                                                                           <  SRR4302534.4462870/94‑1 (MQ=60)
    GTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AACAG                                                                                           >  SRR4302534.5481853/1‑94 (MQ=60)
      GGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AACAGAC                                                                                         <  SRR4302534.4661333/94‑1 (MQ=60)
       GATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AACAGACG                                                                                        >  SRR4302534.2213793/1‑94 (MQ=60)
           TGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAGCCGTGAATTTCCGTTAGAACAA‑AACAGACGATTG                                                                                    <  SRR4302534.4946672/94‑1 (MQ=60)
           TGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AACAGACGATTG                                                                                    <  SRR4302534.5421473/94‑1 (MQ=60)
            GAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AACAGACGATTGT                                                                                   >  SRR4302534.3176131/1‑94 (MQ=60)
             AATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AACAGACGATTGTA                                                                                  >  SRR4302534.3873450/1‑94 (MQ=60)
              ATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AACAGACGATTGTAT                                                                                 <  SRR4302534.2985395/94‑1 (MQ=60)
              ATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AACAGACGATTGTAT                                                                                 <  SRR4302534.3206179/94‑1 (MQ=60)
              ATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AACAGACGATTGTAT                                                                                 <  SRR4302534.5537236/94‑1 (MQ=60)
                NCGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGANTTTCCGTTAGAAC‑ATAACAGACGATTGTATGC                                                                               >  SRR4302534.6561946/1‑94 (MQ=60)
                ACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AACAGACGATTGTATGC                                                                               >  SRR4302534.3870076/1‑94 (MQ=60)
                 CGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AACAGACGATTGTATGCC                                                                              <  SRR4302534.329895/94‑1 (MQ=60)
                  GAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AACAGACGATTGTATGCCT                                                                             >  SRR4302534.4528790/1‑94 (MQ=60)
                  GAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AACAGACGATTGTATGCCT                                                                             >  SRR4302534.637677/1‑94 (MQ=60)
                  GAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AACAGACGATTGTATGCCT                                                                             <  SRR4302534.6391840/94‑1 (MQ=60)
                   AAGAAATACGCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AACAGACGATTGTATGCCTG                                                                            <  SRR4302534.3968463/94‑1 (MQ=60)
                   AAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AACAGACGATTGTATGCCTG                                                                            <  SRR4302534.2028349/94‑1 (MQ=60)
                   AAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AACAGACGATTGTATGCCTG                                                                            >  SRR4302534.3153744/1‑94 (MQ=60)
                    AGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AACAGACGATTGTATGCCTGG                                                                           >  SRR4302534.3817780/1‑94 (MQ=60)
                     GAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AACAGACGATTGTATGCCTGGG                                                                          <  SRR4302534.838314/94‑1 (MQ=60)
                     GAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AACAGACGATTGTATGCCTGGG                                                                          <  SRR4302534.5965642/94‑1 (MQ=60)
                      AAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AACAGACGATTGTATGCCTGGGA                                                                         >  SRR4302534.1440704/1‑94 (MQ=60)
                       AATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AACAGACGATTGTATGCCTGGGAT                                                                        >  SRR4302534.4321680/1‑94 (MQ=60)
                       AATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AACAGACGATTGTATGCCTGGGAT                                                                        >  SRR4302534.2753669/1‑94 (MQ=60)
                       AATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AACAGACGATTGTATGCCTGGGAT                                                                        >  SRR4302534.2878802/1‑94 (MQ=60)
                       AATACCAATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AACAGACGATTGTATGCCTGGGAT                                                                        <  SRR4302534.6009837/94‑1 (MQ=60)
                        ATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AACAGACGATTGTATGCCTGGGATA                                                                       <  SRR4302534.1656849/94‑1 (MQ=60)
                        ATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AACAGACGATTGTATGCCTGGGATA                                                                       <  SRR4302534.5903733/94‑1 (MQ=60)
                         TACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AACAGACGATTGTATGCCTGGGATAT                                                                      >  SRR4302534.6648385/1‑94 (MQ=60)
                         TACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AACAGACGATTGTATGCCTGGGATAT                                                                      <  SRR4302534.1153851/94‑1 (MQ=60)
                         TACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AACAGACGATTGTATGCCTGGGATAT                                                                      <  SRR4302534.1184514/94‑1 (MQ=60)
                           CCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AACAGACGATTGTATGCCTGGGATATTC                                                                    >  SRR4302534.841903/1‑94 (MQ=60)
                           CCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AACAGACAATTGTATGCCGGGGATATTC                                                                    <  SRR4302534.6480399/94‑1 (MQ=60)
                            CCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AACAGACGATTGTATGCCTGGGATATTCT                                                                   <  SRR4302534.6165585/94‑1 (MQ=60)
                            CCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AACAGACGATTGTATGCCTGGGATATTCT                                                                   <  SRR4302534.2726908/94‑1 (MQ=60)
                            CCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AACAGACGATTGTATGCCTGGGATATTCT                                                                   <  SRR4302534.251653/94‑1 (MQ=60)
                             CATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AACAGACGATTGTATGCCTGGGATATTCTA                                                                  <  SRR4302534.4097081/94‑1 (MQ=60)
                              ATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AACAGACGATTGTATGCCTGGGATATTCTAC                                                                 <  SRR4302534.1357746/94‑1 (MQ=60)
                              ATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AACAGACGATTGTATGCCTGGGATATTCTAC                                                                 >  SRR4302534.1572561/1‑94 (MQ=60)
                                AACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AACAGACGATTGTATGCCTGGGATATTCTACAG                                                               <  SRR4302534.5104043/94‑1 (MQ=60)
                                  CAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AACAGACGATTGTATGCCTGGGATATTCTACAGAA                                                             <  SRR4302534.4748700/94‑1 (MQ=60)
                                   AATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AACAGACGATTGAATGCCTGGGATATTCTACAGAAA                                                            >  SRR4302534.1255618/1‑94 (MQ=60)
                                      AAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAA                                                         >  SRR4302534.4973652/1‑94 (MQ=60)
                                       AATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAAC                                                        >  SRR4302534.3544025/1‑94 (MQ=60)
                                       AATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAAC                                                        >  SRR4302534.5484235/1‑94 (MQ=60)
                                       AATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTACGTTAGAACAA‑AACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAAC                                                        >  SRR4302534.1234033/1‑94 (MQ=60)
                                        ATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATCTCCGTTAGAACAA‑AACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACA                                                       >  SRR4302534.2861597/1‑94 (MQ=60)
                                         TGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAG                                                      >  SRR4302534.5049582/1‑94 (MQ=60)
                                         TGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAG                                                      <  SRR4302534.3105128/94‑1 (MQ=60)
                                          GAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGT                                                     >  SRR4302534.2495795/1‑94 (MQ=60)
                                          GAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGT                                                     >  SRR4302534.6041560/1‑94 (MQ=60)
                                          GAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGT                                                     <  SRR4302534.4298231/94‑1 (MQ=60)
                                           AATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTC                                                    >  SRR4302534.2795450/1‑94 (MQ=60)
                                             TATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTG                                                  <  SRR4302534.593999/94‑1 (MQ=60)
                                             TATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTG                                                  >  SRR4302534.1864687/1‑94 (MQ=60)
                                               TAACTCATTCCTGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGAC                                                >  SRR4302534.42256/1‑94 (MQ=60)
                                               TAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGAC                                                >  SRR4302534.1771024/1‑94 (MQ=60)
                                                AACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACA                                               >  SRR4302534.5854339/1‑94 (MQ=60)
                                                AACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACA                                               <  SRR4302534.2298801/94‑1 (MQ=60)
                                                  CTCATTCATGGATTTCAAAACCCGTGAATTTCCGTTAGAACAA‑AACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATA                                             <  SRR4302534.2650587/94‑1 (MQ=60)
                                                  CTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATA                                             <  SRR4302534.176024/94‑1 (MQ=60)
                                                  CTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATA                                             >  SRR4302534.3041453/1‑94 (MQ=60)
                                                  CTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATA                                             >  SRR4302534.264666/1‑94 (MQ=60)
                                                  CTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATA                                             >  SRR4302534.4130166/1‑94 (MQ=60)
                                                    CATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACC                                           <  SRR4302534.4350423/94‑1 (MQ=60)
                                                    CATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACC                                           <  SRR4302534.1715369/94‑1 (MQ=60)
                                                     ATTCATGGATTTCAAAAACCGTGAATTTCTGTTAGAACAA‑AACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCG                                          <  SRR4302534.6524249/94‑1 (MQ=60)
                                                     ATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCG                                          <  SRR4302534.5612806/94‑1 (MQ=60)
                                                      TTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGA                                         <  SRR4302534.3022676/94‑1 (MQ=60)
                                                      TTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGA                                         >  SRR4302534.5927471/1‑94 (MQ=60)
                                                       TCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AACAGACGATTGTATGCCTGGGATATTCTACAGCAAAAACAGTCTGACATACCGAA                                        >  SRR4302534.1988869/1‑94 (MQ=60)
                                                       TCATCGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAA                                        <  SRR4302534.5774802/94‑1 (MQ=60)
                                                        CATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAA                                       >  SRR4302534.6295665/1‑94 (MQ=60)
                                                          TGGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAG                                     >  SRR4302534.3311211/1‑94 (MQ=60)
                                                           GGATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGG                                    >  SRR4302534.234778/1‑94 (MQ=60)
                                                             ATTTCAAAAACCGTGAATTTCCGTTAGAACAA‑AACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGAT                                  >  SRR4302534.2560478/1‑94 (MQ=60)
                                                               TTCAAAAACCGTGAATTTCCGTTAGAACAA‑AACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTA                                <  SRR4302534.1655936/94‑1 (MQ=60)
                                                                  AAAAACCGTGAATTTCCGTTAGAACAA‑AACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAG                             >  SRR4302534.2541431/1‑94 (MQ=60)
                                                                   AAAACCGTGAATTTCCGTTAGAACAA‑AACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGG                            >  SRR4302534.741072/1‑94 (MQ=60)
                                                                     AACCGTGAATTTCCGTTAGAACAA‑AACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTT                          >  SRR4302534.1478706/1‑94 (MQ=60)
                                                                     AACCGTGAATTTCCGTTAGAACAA‑AACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTT                          >  SRR4302534.816433/1‑94 (MQ=60)
                                                                     AACCGTGAATTTCCGTTAGAACAA‑AACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTT                          >  SRR4302534.5905550/1‑94 (MQ=60)
                                                                       CCGTGAATTTCCGTTAGAACAA‑AACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTAT                        <  SRR4302534.3677826/94‑1 (MQ=60)
                                                                        CGTGAATTTCCGTTAGAACAA‑AACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATA                       <  SRR4302534.3201891/94‑1 (MQ=60)
                                                                        CGTGAATTTCCGTTAGAACAA‑AACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATA                       >  SRR4302534.3881978/1‑94 (MQ=60)
                                                                         GTGAATTTCCGTTAGAACAA‑AACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATAT                      <  SRR4302534.2875443/94‑1 (MQ=60)
                                                                          TGAATTTCCGTTAGAACAA‑AACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATT                     <  SRR4302534.1762589/94‑1 (MQ=60)
                                                                           GAATTTCCGTTAGAACAA‑AACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTC                    >  SRR4302534.4110958/1‑94 (MQ=60)
                                                                           GAATTTCCGTTAGAACAA‑AACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTC                    <  SRR4302534.3372039/94‑1 (MQ=60)
                                                                            AATTTCCGTTAGAACAA‑AACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCA                   <  SRR4302534.4930174/94‑1 (MQ=60)
                                                                            AATTTCCGTTAGAACAA‑AACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCA                   >  SRR4302534.2331813/1‑94 (MQ=60)
                                                                               TTCCGTTAGAACAA‑AACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCA                >  SRR4302534.6501578/1‑94 (MQ=60)
                                                                               TTCCGTTAGAACAA‑AACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCA                >  SRR4302534.2630768/1‑94 (MQ=60)
                                                                                TCCGTTAGAACAA‑AACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAG               <  SRR4302534.412938/94‑1 (MQ=60)
                                                                                   GTTAGAACAA‑AACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCG            >  SRR4302534.2861392/1‑94 (MQ=60)
                                                                                   GTTAGAACAA‑AACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCG            >  SRR4302534.1187495/1‑94 (MQ=60)
                                                                                    TTAGAACAA‑AACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGA           <  SRR4302534.3198679/94‑1 (MQ=60)
                                                                                        AACAA‑AACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGG       >  SRR4302534.5890603/1‑94 (MQ=60)
                                                                                          A‑A‑AACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGA    <  SRR4302534.1571508/94‑1 (MQ=60)
                                                                                            A‑AACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGAC   <  SRR4302534.1089566/94‑1 (MQ=60)
                                                                                              AACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACG  >  SRR4302534.721161/1‑94 (MQ=60)
                                                                                              AACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACG  >  SRR4302534.920394/1‑94 (MQ=60)
                                                                                               |                                                                                            
GGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAAC‑A‑AACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACG  >  NC_000913/4435591‑4435776

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑