Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	884	257.2	12958910	96.9%	12557183	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,485,284	T→C	R133R (CGT→CGC)	b4262 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,485,284	0	T	C	99.6%	877.7 / NA	253	R133R (CGT→CGC)	b4262	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (132/120); total (132/121)
Fisher's exact test for biased strand distribution p-value = 4.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 265 total aligned reads displayed.

CTGTCGGTGATGAAAACCGCCATTCCGCTGGTCTTGCTGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGTAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGCAAGGCTTATGGGCGAAAGATGGCAACAACTTCGTCTACAT  >  NC_000913/4485195‑4485373

cTGTCGGTGATGAAAACCGCCATTCCGCTGGTCTTGCTGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAAc                                                                                        >  1:4837899/1‑93 (MQ=255)

cTGTCGGTGATGAAAACCGCCATTCCGCTGGTCTTGCTGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAAc                                                                                        <  2:4619741/93‑1 (MQ=255)

 tGTCGGTGATGAAAACCGCCATTCCGCTGGTCTTGCTGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACt                                                                                       >  2:117246/1‑93 (MQ=255)

 tGTCGGTGATGAAAACCGCCATTCCGCTGGTCTTGCTGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACt                                                                                       <  1:262374/93‑1 (MQ=255)

    cGGTGATGAAAACCGCCATTCCGCTGGTCTTGCTGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTAcc                                                                                    <  1:3876317/93‑1 (MQ=255)

    cGGTGATGAAAACCGCCATTCCGCTGGTCTTGCTGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTAcc                                                                                    <  2:3426206/93‑1 (MQ=255)

      gTGATGAAAACCGCCATTCCGCTGGTCTTGCTGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGt                                                                                  <  2:5669985/93‑1 (MQ=255)

        gatgaAAACCGCCATTCCGCTGGTCTTGCTGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTAGCGTgc                                                                                >  1:2286762/1‑93 (MQ=255)

        gatgaAAACCGCCATTCCGCTGGTCTTGCTGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTgc                                                                                >  1:3971208/1‑93 (MQ=255)

           gaAAACCGCCATTCCGCTGGTCTTGCTGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCa                                                                             >  1:566752/1‑93 (MQ=255)

            aaaaCCGCCATTCCGCTGGTCTTGCTGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAg                                                                            <  1:5186128/93‑1 (MQ=255)

            aaaaCCGCCATTCCGCTGGTCTTGCTGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAg                                                                            <  2:4983608/93‑1 (MQ=255)

              aaCCGCCATTCCGCTGGTCTTGCTGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCCACTACCGTGCGCAggg                                                                          >  2:70583/1‑92 (MQ=255)

              aaCCGCCATTCCGCTGGTCTTGCTGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGc                                                                          >  2:5198780/1‑93 (MQ=255)

              aaCCGCCATTCCGCTGGTCTTGCTGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGc                                                                          >  1:2337777/1‑93 (MQ=255)

              aaCCGCCATTCCGCTGGTCTTGCTGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGc                                                                          <  2:4824563/93‑1 (MQ=255)

              aaCCGCCATTCCGCTGGTCTTGCTGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGc                                                                          <  1:1498637/93‑1 (MQ=255)

               aCCGCCATTCCGCTGGTCTTGCTGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCg                                                                         <  1:4570661/93‑1 (MQ=255)

                ccGCCATTCCGCTGGTCTTGCTGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGa                                                                        >  2:3247035/1‑93 (MQ=255)

                 cGCCATTCCGCTGGTCTTGCTGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGAt                                                                       >  2:1365379/1‑93 (MQ=255)

                  gCCATTCCGCTGGTCTTGCTGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATg                                                                      >  2:3748855/1‑93 (MQ=255)

                   ccATTCCGCTGGTCTTGCTGACGATGCCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGt                                                                     <  2:4575007/93‑1 (MQ=255)

                    cATTCCGCTGGTCTTGCTGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTa                                                                    >  1:4976169/1‑93 (MQ=255)

                    cATTCCGCTGGTCTTGCTGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTa                                                                    >  2:3980437/1‑93 (MQ=255)

                     aTTCCGCTGGTCTTGCTGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTAc                                                                   >  2:2598964/1‑93 (MQ=255)

                     aTTCCGCTGGTCTTGCTGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTAc                                                                   >  2:2704863/1‑93 (MQ=255)

                     aTTCCGCTGGTCTTGCTGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTAc                                                                   >  2:6459838/1‑93 (MQ=255)

                     aTTCCGCTGGTCTTGCTGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTAc                                                                   >  1:1360404/1‑93 (MQ=255)

                     aTTCCGCTGGTCTTGCTGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTAc                                                                   >  2:5520355/1‑93 (MQ=255)

                     aTTCCGCTGGTCGTGCTGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTAc                                                                   >  2:3465136/1‑93 (MQ=255)

                       tCCGCTGGTCTTGCTGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTAcgg                                                                 >  2:4111093/1‑93 (MQ=255)

                         cGCTGGTCTTGCTGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTAcggcg                                                               >  2:3205150/1‑93 (MQ=255)

                           cTGGTCTTGCTGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACggcggc                                                             >  1:5369357/1‑93 (MQ=255)

                           cTGGTCTTGCTGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACggcggc                                                             <  2:616882/93‑1 (MQ=255)

                           cTGGTCTTGCTGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACggcggc                                                             >  2:3336208/1‑93 (MQ=255)

                           cTGGTCTTGCTGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACggcggc                                                             <  1:6194433/93‑1 (MQ=255)

                            tGGTCTTGCTGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCt                                                            >  2:1655309/1‑93 (MQ=255)

                            tGGTCTTGCTGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCt                                                            <  1:5267547/93‑1 (MQ=255)

                               tCTTGCTGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGt                                                         >  1:2575128/1‑93 (MQ=255)

                                cTTGCTGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGtt                                                        <  2:3006009/93‑1 (MQ=255)

                                cTTGCTGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGtt                                                        <  1:1871284/93‑1 (MQ=255)

                                 ttGCTGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTg                                                       >  2:188529/1‑93 (MQ=255)

                                    cTGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGctc                                                    >  1:5924157/1‑93 (MQ=255)

                                    cTGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGctc                                                    <  2:5782864/93‑1 (MQ=255)

                                    cTGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGctc                                                    <  1:4554030/93‑1 (MQ=255)

                                     tGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGctct                                                   >  2:4635815/1‑93 (MQ=255)

                                      gACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGctctt                                                  >  1:6108689/1‑92 (MQ=255)

                                       aCGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGctctct                                                 >  2:1067373/1‑93 (MQ=255)

                                        cGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTa                                                >  1:651022/1‑93 (MQ=255)

                                         gATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTAc                                               <  1:4066986/93‑1 (MQ=255)

                                          aTGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTAcc                                              >  2:5425767/1‑93 (MQ=255)

                                           tGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTAccc                                             <  1:5121935/93‑1 (MQ=255)

                                           tGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTAccc                                             <  1:129709/93‑1 (MQ=255)

                                             gCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAg                                           <  1:1889685/93‑1 (MQ=255)

                                              cGATTGGTGAAGGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGc                                          <  1:4192684/93‑1 (MQ=255)

                                              cGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGc                                          <  1:2958762/93‑1 (MQ=255)

                                                 ttGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGCAAg                                       <  1:5999055/93‑1 (MQ=255)

                                                 ttGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGCAAg                                       >  1:5905963/1‑93 (MQ=255)

                                                 ttGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGCAAg                                       >  2:1532038/1‑93 (MQ=255)

                                                  tGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGCAAgg                                      >  2:2341134/1‑93 (MQ=255)

                                                   ggCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGTAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGCAAGGc                                     <  2:727995/93‑1 (MQ=255)

                                                    gCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGCAAGGCt                                    <  1:2983736/93‑1 (MQ=255)

                                                    gCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGCAAGGCt                                    <  1:4763420/93‑1 (MQ=255)

                                                    gCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGCAAGGCt                                    <  2:93338/93‑1 (MQ=255)

                                                      gAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGCAAGGCTTa                                  >  1:551574/1‑93 (MQ=255)

                                                      gAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGCAAGGCTTa                                  <  1:642410/93‑1 (MQ=255)

                                                       aaTGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGCAAGGCTTAt                                 >  2:1802381/1‑93 (MQ=255)

                                                       aaTGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGCAAGGCTTAt                                 <  1:4276154/93‑1 (MQ=255)

                                                        aTGGGTCGCGCCGCAGGGGGGGCAGATGGCGGGCACCTACCGTGGCCAGGGGTGGACCGGCGCCCGGTTGCTTCCTACCCACAAGGGCTTTTg                                >  2:387347/1‑93 (MQ=255)

                                                        aTGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGCAAGGCTTATg                                >  2:2844056/1‑93 (MQ=255)

                                                        aTGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGCAAGGCTTATg                                <  1:999679/93‑1 (MQ=255)

                                                          gggTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGCAAGGCTTATggg                              >  1:3780954/1‑93 (MQ=255)

                                                           ggTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGCAAGGCTTATGGGc                             >  2:4535601/1‑93 (MQ=255)

                                                           ggTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGCAAGGCTTATGGGc                             <  2:2047443/93‑1 (MQ=255)

                                                            gTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGCAAGGCTTATGGGCg                            <  1:1168939/93‑1 (MQ=255)

                                                               gcgcCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGCAAGGCTTATGGGCGaaa                         <  2:5852319/93‑1 (MQ=255)

                                                               gcgcCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGCAAGGCTTATGGGCGaaa                         <  2:1488631/93‑1 (MQ=255)

                                                                cgccgcAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGCAAGGCTTATGGGCGAAAg                        <  2:4596966/93‑1 (MQ=255)

                                                                 gccgcAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGCAAGGCTTATGGGCGAAAGa                       >  1:2094318/1‑93 (MQ=255)

                                                                  ccgcAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGCAAGGCTTATGGGCGAAAGAt                      <  1:1699801/93‑1 (MQ=255)

                                                                   cgcAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGCAAGGCTTATGGGCGAAAGATg                     <  1:1082559/93‑1 (MQ=255)

                                                                   cgcAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGCAAGGCTTATGGGCGAAAGATg                     >  2:5804459/1‑93 (MQ=255)

                                                                    gcAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGCAAGGCTTATGGGCGAAAGATgg                    >  2:2368359/1‑93 (MQ=255)

                                                                    gcAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGCAAGGCTTATGGGCGAAAGATgg                    >  2:1331394/1‑93 (MQ=255)

                                                                     cAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGCAAGGCTTATGGGCGAAAGATGGc                   >  2:4059289/1‑93 (MQ=255)

                                                                       gggCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGCAAGGCTTATGGGCGAAAGATGGcaa                 >  1:4373839/1‑93 (MQ=255)

                                                                       gggCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGCAAGGCTTATGGGCGAAAGATGGcaa                 >  1:431792/1‑93 (MQ=255)

                                                                        ggCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGCAAGGCTTATGGGCGAAAGATGGcaac                >  1:253224/1‑93 (MQ=255)

                                                                          ggAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGCAAGGCTTATGGGCGAAAGATGGcaacaa              <  1:3511428/92‑1 (MQ=255)

                                                                           gAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGCAAGGCTTATGGGCGAAAGATGGCaacaac             <  2:6341818/93‑1 (MQ=255)

                                                                            aGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGCAAGGCTTATGGGCGAAAGATGGCAACAACt            <  2:6387659/93‑1 (MQ=255)

                                                                             gCAGATCGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGCAAGGCTTATGGGCGAAAGATGGCAACAACtt           <  2:154004/93‑1 (MQ=255)

                                                                               aGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGCAAGGCTTATGGGCGAAAGATGGCAACAACTTCg         >  1:2176233/1‑93 (MQ=255)

                                                                               aGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGCAAGGCTCATGGGCGAAAGATGGCAACAACTTCg         >  2:2623443/1‑93 (MQ=255)

                                                                                gATGGCTCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGCAAGGCTTATGGGCGAAAGATGGCAACAACTTCGt        <  2:1235521/93‑1 (MQ=255)

                                                                                 aTGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGCAAGGCTTATGGGCGAAAGATGGCAACAACTTCGTc       >  2:287297/1‑93 (MQ=255)

                                                                                     cgcgCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGCAAGGCTTATGGGCGAAAGATGGCAACAACTTCGTCTACa   <  1:2725903/93‑1 (MQ=255)

                                                                                      gcgcAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGCAAGGCTTATGGGCGAAAGATGGCAACAACTTCGTCTACAt  >  1:660478/1‑93 (MQ=255)

                                                                                      gcgcAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGCAAGGCTTATGGGCGAAAGATGGCAACAACTTCGTCTACAt  >  2:4362730/1‑93 (MQ=255)

                                                                                      gcgcAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGCAAGGCTTATGGGCGAAAGATGGCAACAACTTCGTCTACAt  <  1:1770507/93‑1 (MQ=255)

CTGTCGGTGATGAAAACCGCCATTCCGCTGGTCTTGCTGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGTAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGCAAGGCTTATGGGCGAAAGATGGCAACAACTTCGTCTACAT  >  NC_000913/4485195‑4485373

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GGCGCTGTCGGTGATGAAAACCGCCATTCCGCTGGTCTTGCTGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGTAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGCAAGGCTTATGGGCGAAAGATGGCAACAACTTCGTCTACATTGAGC  >  NC_000913/4485191‑4485378

GGCGCTGTCGGTGATGAAAACCGCCATTCCGCTGGTCATGCTGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGC                                                                                                >  SRR4302534.6582895/1‑94 (MQ=60)

   GCTGTCGGTGATGAAAACCGCCATTCCGCTGGTCTTGCTGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAAC                                                                                             >  SRR4302534.4969139/1‑94 (MQ=60)

   GCTGTCGGTGATGAAAACCGCCATTCCGCTGGTCTTGCTGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAAC                                                                                             >  SRR4302534.5895561/1‑94 (MQ=60)

   GCTGTCGGTGATGAAAACCGCCATTCCGCTGGTCTTGCTGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAAC                                                                                             >  SRR4302534.1921364/1‑94 (MQ=60)

    CTGTCGGTGATGAAAACCGCCATTCCGCTGGTCTTGCTGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACT                                                                                            <  SRR4302534.5951846/94‑1 (MQ=60)

     TGTCGGTGATGAAAACCGCCATTCCGCTGGTCTTGCTGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTA                                                                                           <  SRR4302534.269182/94‑1 (MQ=60)

        CGGTGATGAAAACCGCCATTCCGCTGGTCTTGCTGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCG                                                                                        <  SRR4302534.3980395/94‑1 (MQ=60)

         GGTGATGAAAACCGCCATTCCGCTGGTCTTGCTGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGT                                                                                       >  SRR4302534.2730762/1‑94 (MQ=60)

           TGATGAAAACCGCCATTCCGCTGGTCTTGCTGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTAGCGTGC                                                                                     >  SRR4302534.2347450/1‑94 (MQ=60)

           TGATGAAAACCGCCATTCCGCTGGTCTTGCTGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGC                                                                                     >  SRR4302534.4077502/1‑94 (MQ=60)

              TGAAAACCGCCATTCCGCTGGTCTTGCTGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCA                                                                                  >  SRR4302534.580670/1‑94 (MQ=60)

                AAAACCGCCATTCCGCTGGTCTTGCTGATGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGG                                                                                <  SRR4302534.4316951/94‑1 (MQ=60)

                AAAACCGCCATTCCGCTGGTCTTGCTGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGG                                                                                <  SRR4302534.3319223/94‑1 (MQ=60)

                AAAACCGCCATTCCGCTGGTCTTGCTGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGG                                                                                <  SRR4302534.5325917/94‑1 (MQ=60)

                 AAACCGCCATTCCGCTGGTCTTGCTGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGC                                                                               >  SRR4302534.2399817/1‑94 (MQ=60)

                  AACCGCCATTCCGCTGGTCTTGCTGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCG                                                                              <  SRR4302534.1538082/94‑1 (MQ=60)

                   ACCGCCATTCCGCTGGTCTTGCTGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGA                                                                             <  SRR4302534.4694581/94‑1 (MQ=60)

                     CGCCATTCCGCTGGTCTTGCTGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATG                                                                           >  SRR4302534.1378627/1‑94 (MQ=60)

                       CCATTCCGCTGGTCTTGCTGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGGGCGCAACTACCGTGCGCAGGCGCTGTA                                                                         >  SRR4302534.5378880/1‑94 (MQ=60)

                       CCATTCCGCTGGTCTTGCTGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTA                                                                         <  SRR4302534.6413057/94‑1 (MQ=60)

                       CCATTCCGCTGGTCTTGCTGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTA                                                                         >  SRR4302534.1453376/1‑94 (MQ=60)

                       CCATTCCGCTGGTCTTGCTGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTA                                                                         >  SRR4302534.5110870/1‑94 (MQ=60)

                       CCATTCCGCTGGTCTTGCTGACGATGGCGATTGGCGAATGGATCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGgc                                                                         >  SRR4302534.3990310/1‑92 (MQ=60)

                        CATTCCGCTGGTCTTGCTGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTAC                                                                        >  SRR4302534.4534349/1‑94 (MQ=60)

                        CATTCCGCTGGTCTTGCTGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTAC                                                                        >  SRR4302534.2203664/1‑94 (MQ=60)

                        CATTCCGCTGGTCTTGCTGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTAC                                                                        >  SRR4302534.1396243/1‑94 (MQ=60)

                         ATTCCGCTGGTCTTGCTGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACG                                                                       <  SRR4302534.616902/94‑1 (MQ=60)

                         ATGCCGTTGGTCTTGCTGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACG                                                                       <  SRR4302534.4736083/94‑1 (MQ=60)

                          TTCCGCTGGTCTTGCTGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGG                                                                      >  SRR4302534.3051610/1‑94 (MQ=60)

                          TTCCGCTGGTCTTGCTGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGG                                                                      <  SRR4302534.4205649/94‑1 (MQ=60)

                             CGCTGGTCTTGCTGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGG                                                                   >  SRR4302534.530086/1‑94 (MQ=60)

                             CGCTGGTCTTGCTGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGG                                                                   <  SRR4302534.2398956/94‑1 (MQ=60)

                              GCTGGTCTTGCTGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGCTGTACGGCGGC                                                                  >  SRR4302534.1775572/1‑94 (MQ=60)

                              GCTGGTCTTGCTGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGC                                                                  >  SRR4302534.5514053/1‑94 (MQ=60)

                              GCTGGTCTTGCTGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGC                                                                  >  SRR4302534.3026496/1‑94 (MQ=60)

                              GCTGGTCTTGCTGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACAGCTGC                                                                  >  SRR4302534.6374518/1‑94 (MQ=60)

                               CTGGTCTTGCTGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCT                                                                 <  SRR4302534.6362982/94‑1 (MQ=60)

                                TGGTCTTGCTGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTC                                                                <  SRR4302534.5409834/94‑1 (MQ=60)

                                TGGTCTTGCTGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTC                                                                >  SRR4302534.1552540/1‑94 (MQ=60)

                                  GTCTTGCTGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGT                                                              >  SRR4302534.2643345/1‑94 (MQ=60)

                                    CTTGCTGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTG                                                            <  SRR4302534.1919974/94‑1 (MQ=60)

                                    CTTGCTGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTG                                                            >  SRR4302534.69352/1‑94 (MQ=60)

                                    CTTGCTGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTG                                                            <  SRR4302534.6074188/94‑1 (MQ=60)

                                    CTTGCGGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTG                                                            <  SRR4302534.4394838/94‑1 (MQ=60)

                                     ttacgGACGATGGCGATTGTCGAATGGGTCGCACCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGC                                                           <  SRR4302534.913789/89‑1 (MQ=60)

                                     TTGCTGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGC                                                           >  SRR4302534.2623909/1‑94 (MQ=60)

                                       GCTGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTC                                                         >  SRR4302534.6276189/1‑94 (MQ=60)

                                       GCTGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTC                                                         >  SRR4302534.6085014/1‑94 (MQ=60)

                                        CTGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCT                                                        <  SRR4302534.4677482/94‑1 (MQ=60)

                                        CTGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCT                                                        >  SRR4302534.5000358/1‑94 (MQ=60)

                                         TGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTT                                                       >  SRR4302534.6274812/1‑94 (MQ=60)

                                         TGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTC                                                       >  SRR4302534.2256391/1‑94 (MQ=60)

                                          GACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCT                                                      <  SRR4302534.1985797/94‑1 (MQ=60)

                                           ACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTA                                                     >  SRR4302534.4082732/1‑94 (MQ=60)

                                           ACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTA                                                     <  SRR4302534.4178544/94‑1 (MQ=60)

                                           ACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTA                                                     <  SRR4302534.1913208/94‑1 (MQ=60)

                                           ACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTA                                                     >  SRR4302534.666824/1‑94 (MQ=60)

                                            CGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTAC                                                    >  SRR4302534.2346699/1‑94 (MQ=60)

                                             GATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACC                                                   <  SRR4302534.4176101/94‑1 (MQ=60)

                                             GATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACC                                                   >  SRR4302534.2634990/1‑94 (MQ=60)

                                             GATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACC                                                   >  SRR4302534.1476799/1‑94 (MQ=60)

                                             AATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGGGCAGAGGGCGCGCAACTACCGGGCGGAGGCGATGTACGGCGGCTCGTTTCTCTCTACC                                                   >  SRR4302534.1020633/1‑94 (MQ=60)

                                              ATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCC                                                  <  SRR4302534.1938353/94‑1 (MQ=60)

                                              ATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCC                                                  >  SRR4302534.3616427/1‑94 (MQ=60)

                                               TGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCA                                                 <  SRR4302534.5260235/94‑1 (MQ=60)

                                               TGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCA                                                 <  SRR4302534.133137/94‑1 (MQ=60)

                                                GGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAG                                                <  SRR4302534.4683160/94‑1 (MQ=60)

                                                 GCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGC                                               <  SRR4302534.1938966/94‑1 (MQ=60)

                                                  CGATTGGTGAAGGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGCA                                              <  SRR4302534.4305949/94‑1 (MQ=60)

                                                  CGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGCA                                              <  SRR4302534.3036298/94‑1 (MQ=60)

                                                  CGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGCA                                              >  SRR4302534.2229680/1‑94 (MQ=60)

                                                   GATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTCCCCAGCAA                                             <  SRR4302534.6268829/94‑1 (MQ=60)

                                                   GATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGCAA                                             >  SRR4302534.5181970/1‑94 (MQ=60)

                                                   GATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGCAA                                             >  SRR4302534.6608450/1‑94 (MQ=60)

                                                    ATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGCAAG                                            <  SRR4302534.653615/94‑1 (MQ=60)

                                                    ATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGCAAG                                            >  SRR4302534.6066330/1‑94 (MQ=60)

                                                     TTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGCAAGG                                           <  SRR4302534.6162033/94‑1 (MQ=60)

                                                     TTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGCAAGG                                           <  SRR4302534.1297439/94‑1 (MQ=60)

                                                      TGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGCAAGGC                                          >  SRR4302534.4478268/1‑94 (MQ=60)

                                                      TGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGCAAGGC                                          <  SRR4302534.6109302/94‑1 (MQ=60)

                                                      TGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGCAAGGC                                          <  SRR4302534.2930434/94‑1 (MQ=60)

                                                        GCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGCAAGGCTT                                        <  SRR4302534.4892761/94‑1 (MQ=60)

                                                        GCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGCAAGGCTT                                        <  SRR4302534.3061793/94‑1 (MQ=60)

                                                        GCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGCAAGGCTT                                        <  SRR4302534.5557815/94‑1 (MQ=60)

                                                         CGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGCAAGGCTTA                                       >  SRR4302534.565174/1‑94 (MQ=60)

                                                          GAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGCAAGGCTTAT                                      <  SRR4302534.658004/94‑1 (MQ=60)

                                                          GAATGGGGCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGCAAGGCTTAT                                      >  SRR4302534.5505246/1‑94 (MQ=60)

                                                           AATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGCAAGGCTTATG                                     <  SRR4302534.4391833/94‑1 (MQ=60)

                                                            ATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGCAAGGCTTATGG                                    <  SRR4302534.1025303/94‑1 (MQ=60)

                                                            ATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGCAAGGCTTATGG                                    <  SRR4302534.1909140/94‑1 (MQ=60)

                                                             TGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGCAAGGCTTATGGG                                   >  SRR4302534.3882473/1‑94 (MQ=60)

                                                               GGTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGCAAGGCTTATGGGCG                                 >  SRR4302534.2794407/1‑94 (MQ=60)

                                                                GTCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGCAAGGCTTATGGGCGA                                <  SRR4302534.1199642/94‑1 (MQ=60)

                                                                 TCGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGCAAGGCTTATGGGCGAA                               <  SRR4302534.2459944/94‑1 (MQ=60)

                                                                  CGCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGCAAGGCTTATGGGCGAAA                              >  SRR4302534.1616251/1‑94 (MQ=60)

                                                                   GCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGCAAGGCTTATGGGCGAAAG                             <  SRR4302534.6084004/94‑1 (MQ=60)

                                                                   GCGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGCAAGGCTTATGGGCGAAAG                             >  SRR4302534.2656143/1‑94 (MQ=60)

                                                                    CGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGCAAGGCTTATGGGCGAAAGA                            >  SRR4302534.3247714/1‑94 (MQ=60)

                                                                    CGCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGCAAGGCTTATGGGCGAAAGA                            >  SRR4302534.2149253/1‑94 (MQ=60)

                                                                     GCCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGCAAGGCTTATGGGCGAAAGAT                           >  SRR4302534.5733862/1‑94 (MQ=60)

                                                                      CCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGCAAGGCTTATGGGCGAAAGATG                          <  SRR4302534.1743965/94‑1 (MQ=60)

                                                                      CCGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGCTGCTCTCTACCCCGCAAGGCTTATGTGCGCAAGATG                          >  SRR4302534.4041083/1‑94 (MQ=60)

                                                                       CGCAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGCAAGGCTTATGGGCGAAAGATGG                         <  SRR4302534.1110704/94‑1 (MQ=60)

                                                                         CAGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGCAAGGCTTATGGGCGAAAGATGGCA                       <  SRR4302534.4655/94‑1 (MQ=60)

                                                                         CAGGCCGAGCAGATGGCGCGGAACTACCGTGCGCAGGAAATGTCCGACGGGTCGTTGCTCTATACCCAGCAAGGCTTATGGGCGAAAGATGGCA                       <  SRR4302534.1380054/94‑1 (MQ=60)

                                                                          AGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGCAAGGCTTATGGGCGAAAGATGGCAA                      >  SRR4302534.4939213/1‑94 (MQ=60)

                                                                          AGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGCAAGGCTTATGGGCGAAAGATGGCAA                      >  SRR4302534.4491725/1‑94 (MQ=60)

                                                                          AGGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGCAAGGCTTATGGGCGAAAGATGGCAA                      >  SRR4302534.442807/1‑94 (MQ=60)

                                                                           GGGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGCAAGGCTTATGGGCGAAAGATGGCAAC                     >  SRR4302534.259822/1‑94 (MQ=60)

                                                                            GGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGCAAGGCTTATGGGCGAAAGATGGCAACA                    <  SRR4302534.369818/94‑1 (MQ=60)

                                                                            GGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGCAAGGCTTATGGGCGAAAGATGGCAACA                    >  SRR4302534.671971/1‑94 (MQ=60)

                                                                            GGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGCAAGGCTTATGGGCGAAAGATGGCAACA                    <  SRR4302534.4902133/94‑1 (MQ=60)

                                                                            GGCGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGCAAGGCTTATGGGCGAAAGATGGCAACA                    <  SRR4302534.2436808/94‑1 (MQ=60)

                                                                              GGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGCAAGGCTTATGGGCGAAAGATGGCAACAAC                  <  SRR4302534.3605892/94‑1 (MQ=60)

                                                                              CGAGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGCAAGGCTTATGGGCGAAAGATGGCAACAAC                  >  SRR4302534.1216413/1‑94 (MQ=60)

                                                                                AGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGCAAGGCTTATGGGCGAAAGATGGCAACAACTT                >  SRR4302534.446418/1‑94 (MQ=60)

                                                                                AGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGCAAGGCTTATGGGCGAAAGATGGCAACAACTT                >  SRR4302534.4851146/1‑94 (MQ=60)

                                                                                AGCAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGCAAGGCTTATGGGCGAAAGATGGCAACAACTT                >  SRR4302534.3928606/1‑94 (MQ=60)

                                                                                  CAGATGGCGCGCACCTAACGTGCGCAGGCGATGTACGGCGGCTCGTTGGTCTCTACCCAGCAAGGCTTAGGGGCGAAAGATGGCAACAACTTCG              <  SRR4302534.948463/94‑1 (MQ=60)

                                                                                  CAGATGGCGCGCAACTACCGTGTGCAGGCGATGTACGGCGGCTCGGTGCTCGCTACCCAGTAAGGCTTATGGGCGTATGACGGCAACAACTTCG              >  SRR4302534.6649326/1‑94 (MQ=60)

                                                                                  CAGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGCAAGGCTTATGGGCGAAAGATGGCAACAACTTCG              >  SRR4302534.2233728/1‑94 (MQ=60)

                                                                                   AGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGCAAGGCTTATGGGCGAAAGATGGCAACAACTTCGT             >  SRR4302534.1131201/1‑94 (MQ=60)

                                                                                   AGATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGCAAGGCTTATGGGCGAAAGATGGCAACAACTTCGT             >  SRR4302534.5273524/1‑94 (MQ=60)

                                                                                    GATGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGCAAGGCTTATGGGCGAAAGATGGCAACAACTTCGTC            <  SRR4302534.4069795/94‑1 (MQ=60)

                                                                                      TGGCGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGCAAGGCTTATGGGCGAAAGATGGCAACAACTTCGTCTA          <  SRR4302534.513229/94‑1 (MQ=60)

                                                                                       GGCGC‑‑AACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGCAAGGCTTATGGGCGAAAGATGGCAACAACTTCGTCTACAT       <  SRR4302534.5453417/94‑1 (MQ=60)

                                                                                         CGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGCAAGGCTTATGGGCGAAAGATGGCAACAACTTCGTCTACAT       <  SRR4302534.2797807/94‑1 (MQ=60)

                                                                                         CGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGCAAGGCTTATGGGCGAAAGATGGCAACAACTTCGTCTACAT       >  SRR4302534.676534/1‑94 (MQ=60)

                                                                                         CGCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGCAAGGCTTATGGGCGAAAGATGGCAACAACTTCGTCTACAT       >  SRR4302534.5621339/1‑94 (MQ=60)

                                                                                          GCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGCAAGGCTTATGGGCGAAAGATGGCAACAACTTCGTCTACATT      <  SRR4302534.1816476/94‑1 (MQ=60)

                                                                                          GCGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGCAAGGCTTATGGGCGAAAGATGGCAACAACTTCGTCTACATT      >  SRR4302534.5059987/1‑94 (MQ=60)

                                                                                           CGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGCAAGGCTTATGGGCTAAAGATGGCAACAACTTCGTCTACATTG     <  SRR4302534.3753934/94‑1 (MQ=60)

                                                                                           CGCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGCAAGGCTTATGGGCGAAAGATGGCAACAACTTCGTCTACATTG     >  SRR4302534.6205723/1‑94 (MQ=60)

                                                                                            GCAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGCAAGGCTTATGGGCGAAAGATGGCAACAACTTCGTCTACATTGA    <  SRR4302534.6617141/94‑1 (MQ=60)

                                                                                             CAACTACCGTGCGCAGGCGATGTAC‑GCGGCTCGTTGCTCTCTACCCAGCAAGGCTTATGGGCGAAAGATGGCAACAAATTCGTCTACATTtagg  >  SRR4302534.2354448/1‑90 (MQ=60)

GGCGCTGTCGGTGATGAAAACCGCCATTCCGCTGGTCTTGCTGACGATGGCGATTGGCGAATGGGTCGCGCCGCAGGGCGAGCAGATGGCGCGTAACTACCGTGCGCAGGCGATGTACGGCGGCTCGTTGCTCTCTACCCAGCAAGGCTTATGGGCGAAAGATGGCAACAACTTCGTCTACATTGAGC  >  NC_000913/4485191‑4485378

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`