Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	884	257.2	12958910	96.9%	12557183	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,555,245	T→C	Y205C (TAC→TGC)	yjiE ←	putative transcriptional regulator LYSR‑type

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,555,245	0	T	C	100.0%	881.7 / NA	249	Y205C (TAC→TGC)	yjiE	putative transcriptional regulator LYSR‑type
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (136/113); total (136/113)

Only 100 of 255 total aligned reads displayed.

TTTAAAAGCTCGCTCATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGTAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTCACTGGCGCAGACAGGG  >  NC_000913/4555155‑4555334

       gctcgctcATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGc                                                                                  >  1:1352116/1‑93 (MQ=255)

       gctcgctcATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGAAGGAGCTGc                                                                                  >  1:600276/1‑93 (MQ=255)

          cgctcATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGc                                                                               <  1:5191494/93‑1 (MQ=255)

            ctcATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTg                                                                             <  1:3778997/93‑1 (MQ=255)

            ctcATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTg                                                                             >  1:6184600/1‑93 (MQ=255)

            ctcATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTg                                                                             <  1:6435018/93‑1 (MQ=255)

            ctcATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGCAAGGGTGCGATTAATCAATCGCCCCATCCAGGAGTTGCGCCTg                                                                             >  2:2760766/1‑93 (MQ=255)

             tcATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGt                                                                            <  2:2001984/93‑1 (MQ=255)

             tcATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGt                                                                            >  2:2363865/1‑93 (MQ=255)

             tcATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGt                                                                            <  2:2602243/93‑1 (MQ=255)

              cATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTa                                                                           >  2:3476880/1‑93 (MQ=255)

              cATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTa                                                                           >  2:5797450/1‑93 (MQ=255)

               aTCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTaa                                                                          <  2:5395944/93‑1 (MQ=255)

                tCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAAt                                                                         <  2:2553545/93‑1 (MQ=255)

                tCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAAt                                                                         >  2:2266063/1‑93 (MQ=255)

                tCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAAt                                                                         <  2:1049926/93‑1 (MQ=255)

                 cGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAAtt                                                                        >  1:155375/1‑93 (MQ=255)

                 cGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAAtt                                                                        >  1:5673899/1‑93 (MQ=255)

                 cGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAAtt                                                                        >  1:4284728/1‑93 (MQ=255)

                  gAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTc                                                                       <  1:3245660/93‑1 (MQ=255)

                   aaGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTcc                                                                      >  1:2245635/1‑92 (MQ=255)

                   aaGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCa                                                                      <  1:1197126/93‑1 (MQ=255)

                   aaGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCa                                                                      >  1:4683818/1‑93 (MQ=255)

                   aaGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCa                                                                      <  1:3925668/93‑1 (MQ=255)

                     gagaCAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGt                                                                    >  2:5442942/1‑93 (MQ=255)

                     gagaCAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGt                                                                    >  1:3282190/1‑93 (MQ=255)

                      agaCAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTa                                                                   >  2:2906016/1‑93 (MQ=255)

                      agaCAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTa                                                                   >  1:1890857/1‑93 (MQ=255)

                      agaCAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTa                                                                   >  1:2620650/1‑93 (MQ=255)

                       gaCAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTac                                                                  >  1:4362834/1‑92 (MQ=255)

                        aCAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAAc                                                                 >  1:1988044/1‑93 (MQ=255)

                          aaaaaaGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACgg                                                               >  2:811452/1‑93 (MQ=255)

                          aaaaaaGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACgg                                                               <  2:5939098/93‑1 (MQ=255)

                          aaaaaaGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACgg                                                               >  2:57750/1‑93 (MQ=255)

                           aaaaaGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCACCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGa                                                              >  1:737731/1‑93 (MQ=255)

                            aaaaGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGaa                                                             >  1:4152005/1‑93 (MQ=255)

                              aaGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAg                                                           <  1:2729595/93‑1 (MQ=255)

                              aaGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAg                                                           >  1:1677409/1‑93 (MQ=255)

                              aaGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAg                                                           <  1:4889328/93‑1 (MQ=255)

                               aGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAgg                                                          >  2:4386381/1‑92 (MQ=255)

                                ggTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAgtg                                                         >  1:5838315/1‑93 (MQ=255)

                                  tGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGtgtg                                                       <  2:400330/93‑1 (MQ=255)

                                   gCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTgg                                                      <  2:3062093/93‑1 (MQ=255)

                                   gCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTgg                                                      >  1:2380946/1‑93 (MQ=255)

                                    gtGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGc                                                     <  1:6092369/92‑1 (MQ=255)

                                    cTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGc                                                     <  1:3316462/93‑1 (MQ=255)

                                     tGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCt                                                    <  2:5885986/93‑1 (MQ=255)

                                      gAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTg                                                   <  1:4518332/93‑1 (MQ=255)

                                         aCTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTgcgc                                                >  2:5145673/1‑93 (MQ=255)

                                         aCTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTgcgc                                                >  2:3759835/1‑93 (MQ=255)

                                           ttAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGcgcgg                                              >  2:4944951/1‑92 (MQ=255)

                                            tAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAg                                             <  1:6025780/93‑1 (MQ=255)

                                             aaCTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAgg                                            <  2:3382563/93‑1 (MQ=255)

                                              aCTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGt                                           <  2:760702/93‑1 (MQ=255)

                                              aCTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGt                                           <  1:505906/93‑1 (MQ=255)

                                                tCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCGTGCAGGAGTTGCGGCTGGAATTTAGTAACGGAAAGTGTGGCTGCGCGAGGGTa                                         >  2:2472060/1‑93 (MQ=255)

                                                tCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTa                                         >  2:1723368/1‑93 (MQ=255)

                                                tCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGGTa                                         >  1:4715323/1‑93 (MQ=255)

                                                 cACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTaa                                        >  1:2784859/1‑93 (MQ=255)

                                                 cACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGGTaa                                        >  1:3546164/1‑93 (MQ=255)

                                                   cTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTaaaa                                      >  1:5895654/1‑93 (MQ=255)

                                                    tgtgGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTaaaaa                                     >  1:5735253/1‑93 (MQ=255)

                                                      tgGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGt                                   <  2:3626155/93‑1 (MQ=255)

                                                      tgGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGt                                   <  1:4406797/93‑1 (MQ=255)

                                                       ggCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTg                                  >  1:1259183/1‑93 (MQ=255)

                                                        gcgcGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGc                                 <  2:4519889/93‑1 (MQ=255)

                                                        gcgcGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGc                                 <  2:331828/93‑1 (MQ=255)

                                                          gcgTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCtt                               >  1:4801681/1‑93 (MQ=255)

                                                           cgTCACGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTtc                              <  2:4255740/93‑1 (MQ=255)

                                                            gTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTtct                             >  2:5081111/1‑93 (MQ=255)

                                                            gTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTtct                             >  1:5270487/1‑93 (MQ=255)

                                                             tCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTtctc                            <  2:5230259/93‑1 (MQ=255)

                                                             tCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTtctc                            >  2:1418486/1‑93 (MQ=255)

                                                             tCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTtctc                            <  2:5113095/93‑1 (MQ=255)

                                                             tCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTtctc                            <  1:3055674/93‑1 (MQ=255)

                                                             tCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTtctc                            >  2:2833170/1‑93 (MQ=255)

                                                              cAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTcc                           <  2:569350/93‑1 (MQ=255)

                                                                gggTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCgt                         <  2:468268/93‑1 (MQ=255)

                                                                 ggTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCgtg                        >  1:1726319/1‑93 (MQ=255)

                                                                 ggTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCCTCTCCgtg                        >  2:1491480/1‑93 (MQ=255)

                                                                  gTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCgtgt                       <  1:2560374/93‑1 (MQ=255)

                                                                  gTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCgtgt                       <  1:312437/93‑1 (MQ=255)

                                                                   tGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGtt                      <  2:4973643/93‑1 (MQ=255)

                                                                   tGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGtt                      <  1:1037790/93‑1 (MQ=255)

                                                                    gCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTc                     <  2:3677892/93‑1 (MQ=255)

                                                                      gATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGt                   <  1:2439375/93‑1 (MQ=255)

                                                                       aTTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTc                  >  1:1525885/1‑93 (MQ=255)

                                                                         tAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTCAc                <  2:3225453/93‑1 (MQ=255)

                                                                           atcaatcGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTCACTg              >  1:512683/1‑93 (MQ=255)

                                                                               atcGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTCACTGgcgc          >  2:4703387/1‑93 (MQ=255)

                                                                                tcGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTCACTGGCGCa         >  1:5491920/1‑93 (MQ=255)

                                                                                tcGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTCACTGGCGCa         >  2:2696112/1‑93 (MQ=255)

                                                                                  gCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTCACTGGCGCAGa       <  1:4630472/93‑1 (MQ=255)

                                                                                  gCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTCACTGGCGCAGa       >  2:655452/1‑93 (MQ=255)

                                                                                   ccccATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTCACTGGCGCAGAc      >  1:4831108/1‑93 (MQ=255)

                                                                                    cccATGCAGGAGTTGCGGCTGGAATTCAGCAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCCTGTTCGTCACTGGCGCAGACa     >  2:6159883/1‑93 (MQ=255)

                                                                                     ccATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTCACTGGCGCAGACAg    <  2:191130/93‑1 (MQ=255)

                                                                                      cATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTCACTGGCGCAGACAgg   <  2:2493735/93‑1 (MQ=255)

                                                                                      cATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTCACTGGCGCAGACAgg   <  2:6231319/93‑1 (MQ=255)

                                                                                       aTGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGGTAAAAAATGCTTCTCCGTGTTCGTCACTGGCGCAGACAggg  >  1:3510829/1‑93 (MQ=255)

TTTAAAAGCTCGCTCATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGTAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTCACTGGCGCAGACAGGG  >  NC_000913/4555155‑4555334

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GCTTTAAAAGCTCGCTCATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGTAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTCACTGGCGCAGACAGGGAACA  >  NC_000913/4555153‑4555338

GCTTTAAAAGCTCGCTCATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCA                                                                                              <  SRR4302534.568264/94‑1 (MQ=60)

 CTTTAAAAGCTCGCTCATCGAAGAGACAAAAAAGGGGCTGAAACTTAACTCACTGTGGCGGGTCAGGGTGCGATTAATCAATCGCCCCATGCAG                                                                                             >  SRR4302534.683990/1‑94 (MQ=60)

   TTAAAAGCTCGCTCATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGA                                                                                           >  SRR4302534.739457/1‑94 (MQ=60)

   TTAAAAGCTCGCTCATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGA                                                                                           <  SRR4302534.2040230/94‑1 (MQ=60)

    TAAAAGCTCGCTCATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAG                                                                                          >  SRR4302534.1240358/1‑94 (MQ=60)

      AAAGCTCGCTCATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTT                                                                                        <  SRR4302534.5722221/94‑1 (MQ=60)

      AAAGCTCGCTCATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTT                                                                                        >  SRR4302534.1580615/1‑94 (MQ=60)

       AAGCTCGCTCATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTG                                                                                       >  SRR4302534.594038/1‑94 (MQ=60)

       AAGCTCGCTCATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTG                                                                                       <  SRR4302534.3396656/94‑1 (MQ=60)

        AGCTCGCTCATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGC                                                                                      >  SRR4302534.1387760/1‑94 (MQ=60)

        AGCTCGCTCATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGC                                                                                      >  SRR4302534.6031390/1‑94 (MQ=60)

        AGCTCGCTCATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGC                                                                                      >  SRR4302534.2455238/1‑94 (MQ=60)

        AGCTCGCTCATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGAAGGAGCTGC                                                                                      >  SRR4302534.614914/1‑94 (MQ=60)

         GCTCGCTCATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCG                                                                                     >  SRR4302534.47221/1‑94 (MQ=60)

         GCTCGCTCATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCG                                                                                     <  SRR4302534.2125373/94‑1 (MQ=60)

          CTCGCTCATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGG                                                                                    >  SRR4302534.4455447/1‑94 (MQ=60)

          CTCGCTCATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGG                                                                                    >  SRR4302534.4455446/1‑94 (MQ=60)

           TCGCTCATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGC                                                                                   <  SRR4302534.4588262/94‑1 (MQ=60)

            CGCTCATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCT                                                                                  <  SRR4302534.5331449/94‑1 (MQ=60)

             GCTCATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTG                                                                                 >  SRR4302534.6352932/1‑94 (MQ=60)

             GCTCATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTG                                                                                 >  SRR4302534.524993/1‑94 (MQ=60)

              CTCATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGT                                                                                >  SRR4302534.2778916/1‑94 (MQ=60)

              CTCATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGT                                                                                >  SRR4302534.5273651/1‑94 (MQ=60)

              CTCATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGT                                                                                <  SRR4302534.4253445/94‑1 (MQ=60)

              CTCATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGT                                                                                <  SRR4302534.3880454/94‑1 (MQ=60)

              CTCATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGT                                                                                >  SRR4302534.5938520/1‑94 (MQ=60)

              CTCATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGT                                                                                <  SRR4302534.6608475/94‑1 (MQ=60)

               TCATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCGGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTA                                                                               <  SRR4302534.2577038/94‑1 (MQ=60)

                CATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAA                                                                              >  SRR4302534.2416645/1‑94 (MQ=60)

                  TCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATT                                                                            >  SRR4302534.3141353/1‑94 (MQ=60)

                  TCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATT                                                                            >  SRR4302534.4400769/1‑94 (MQ=60)

                  TCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATT                                                                            >  SRR4302534.5827510/1‑94 (MQ=60)

                  TCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATT                                                                            <  SRR4302534.6255640/94‑1 (MQ=60)

                  TCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATT                                                                            >  SRR4302534.159438/1‑94 (MQ=60)

                   CGGAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTC                                                                           >  SRR4302534.6346044/1‑94 (MQ=60)

                   CGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTC                                                                           >  SRR4302534.3808780/1‑94 (MQ=60)

                   CGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTC                                                                           >  SRR4302534.135478/1‑94 (MQ=60)

                    GAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCC                                                                          >  SRR4302534.2305152/1‑94 (MQ=60)

                    GAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCA                                                                          <  SRR4302534.3332067/94‑1 (MQ=60)

                    GAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCA                                                                          >  SRR4302534.4810980/1‑94 (MQ=60)

                     AAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAG                                                                         >  SRR4302534.4956156/1‑94 (MQ=60)

                     AAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAG                                                                         <  SRR4302534.4030834/94‑1 (MQ=60)

                     AAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAG                                                                         <  SRR4302534.1228631/94‑1 (MQ=60)

                      AGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGT                                                                        >  SRR4302534.3369673/1‑94 (MQ=60)

                       GAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTA                                                                       >  SRR4302534.2689996/1‑94 (MQ=60)

                       GAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTA                                                                       <  SRR4302534.4762495/94‑1 (MQ=60)

                       GAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTA                                                                       >  SRR4302534.1940169/1‑94 (MQ=60)

                        AGACAAAATTGGGGCTGAAACTTATCTCACTGTGGTGCGGCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGCTGCGGCTGctagtcaggaa                                                                      >  SRR4302534.6639118/1‑83 (MQ=49)

                        AGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAC                                                                      >  SRR4302534.4480488/1‑94 (MQ=60)

                         GACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAAC                                                                     >  SRR4302534.2040101/1‑94 (MQ=60)

                           CAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGG                                                                   <  SRR4302534.1418419/94‑1 (MQ=60)

                            AAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGA                                                                  >  SRR4302534.1280270/1‑94 (MQ=60)

                            AAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCACCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGA                                                                  >  SRR4302534.755975/1‑94 (MQ=60)

                             AAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAA                                                                 >  SRR4302534.4264091/1‑94 (MQ=60)

                             AAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAA                                                                 >  SRR4302534.2638490/1‑94 (MQ=60)

                               AAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAG                                                               <  SRR4302534.4264606/94‑1 (MQ=60)

                               AAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAG                                                               >  SRR4302534.1721053/1‑94 (MQ=60)

                                AAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTTAGTAACGGAAAGT                                                              >  SRR4302534.5419494/1‑94 (MQ=60)

                                AAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGT                                                              <  SRR4302534.2801574/94‑1 (MQ=60)

                                AAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGT                                                              <  SRR4302534.5021908/94‑1 (MQ=60)

                                 AGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTG                                                             >  SRR4302534.5996810/1‑94 (MQ=60)

                                    TGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGG                                                          >  SRR4302534.2444211/1‑94 (MQ=60)

                                    TGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGG                                                          <  SRR4302534.483390/94‑1 (MQ=60)

                                    TGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGG                                                          <  SRR4302534.4484967/94‑1 (MQ=60)

                                      GTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCT                                                        <  SRR4302534.6258043/94‑1 (MQ=60)

                                      CTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCT                                                        >  SRR4302534.4724046/1‑94 (MQ=60)

                                      CTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCT                                                        <  SRR4302534.3405104/94‑1 (MQ=60)

                                       TGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTG                                                       <  SRR4302534.6524300/94‑1 (MQ=60)

                                        GAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGC                                                      <  SRR4302534.4640729/94‑1 (MQ=60)

                                         AAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCG                                                     >  SRR4302534.5335507/1‑94 (MQ=60)

                                         AAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCG                                                     <  SRR4302534.5394327/94‑1 (MQ=60)

                                           ACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGCAAGTGTGGCTGTGCG                                                   >  SRR4302534.5069969/1‑94 (MQ=60)

                                           ACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCG                                                   <  SRR4302534.3613684/94‑1 (MQ=60)

                                           ACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCG                                                   <  SRR4302534.1681121/94‑1 (MQ=60)

                                             TTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAG                                                 <  SRR4302534.3332937/94‑1 (MQ=60)

                                              TAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGG                                                <  SRR4302534.6189534/94‑1 (MQ=60)

                                                ACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTT                                              <  SRR4302534.518483/94‑1 (MQ=60)

                                                 CTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTA                                             >  SRR4302534.1904039/1‑94 (MQ=60)

                                                 CTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTA                                             <  SRR4302534.3224048/94‑1 (MQ=60)

                                                 CTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTA                                             >  SRR4302534.6171753/1‑94 (MQ=60)

                                                 CTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGGTA                                             >  SRR4302534.4843367/1‑94 (MQ=60)

                                                  TCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAA                                            >  SRR4302534.2858105/1‑94 (MQ=60)

                                                  TCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAA                                            <  SRR4302534.5640445/94‑1 (MQ=60)

                                                  TCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGGTAA                                            >  SRR4302534.3641539/1‑94 (MQ=60)

                                                    ACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAA                                          >  SRR4302534.482799/1‑94 (MQ=60)

                                                    ACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAA                                          <  SRR4302534.1764610/94‑1 (MQ=60)

                                                    ACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAA                                          >  SRR4302534.6055733/1‑94 (MQ=60)

                                                    ACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGGTAAAA                                          >  SRR4302534.2320153/1‑94 (MQ=60)

                                                    ACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGGTAAAA                                          >  SRR4302534.6098851/1‑94 (MQ=60)

                                                     CTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAA                                         >  SRR4302534.5890665/1‑94 (MQ=60)

                                                     CTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAA                                         >  SRR4302534.1629766/1‑94 (MQ=60)

                                                        TGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTG                                      >  SRR4302534.1292349/1‑94 (MQ=60)

                                                        TGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTG                                      <  SRR4302534.4525718/94‑1 (MQ=60)

                                                          GCGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCT                                    >  SRR4302534.586388/1‑94 (MQ=60)

                                                           CGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTT                                   <  SRR4302534.2798550/94‑1 (MQ=60)

                                                           CGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTT                                   >  SRR4302534.4965002/1‑94 (MQ=60)

                                                           CGCGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTT                                   >  SRR4302534.4932065/1‑94 (MQ=60)

                                                             CGTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCT                                 >  SRR4302534.5412855/1‑94 (MQ=60)

                                                             CGTCAGGGCGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGAGCTTCT                                 >  SRR4302534.1112034/1‑94 (MQ=60)

                                                              GTCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTC                                <  SRR4302534.1393309/94‑1 (MQ=60)

                                                               TCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCC                               <  SRR4302534.3135851/94‑1 (MQ=60)

                                                               TCAGGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGCGCTTCTCC                               >  SRR4302534.6156707/1‑94 (MQ=60)

                                                                  GGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTG                            >  SRR4302534.1771144/1‑94 (MQ=60)

                                                                  GGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTG                            >  SRR4302534.4133/1‑94 (MQ=60)

                                                                  GGGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTG                            >  SRR4302534.3105582/1‑94 (MQ=60)

                                                                   GGTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGT                           <  SRR4302534.6216501/94‑1 (MQ=60)

                                                                    GTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTT                          <  SRR4302534.4952285/94‑1 (MQ=60)

                                                                    GTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTT                          <  SRR4302534.320492/94‑1 (MQ=60)

                                                                    GTGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTT                          <  SRR4302534.2628239/94‑1 (MQ=60)

                                                                     TGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTC                         >  SRR4302534.3581040/1‑94 (MQ=60)

                                                                     TGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTC                         <  SRR4302534.6422947/94‑1 (MQ=60)

                                                                     TGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTC                         <  SRR4302534.1064572/94‑1 (MQ=60)

                                                                     TGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTC                         <  SRR4302534.2857106/94‑1 (MQ=60)

                                                                     GGCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTC                         <  SRR4302534.4616814/94‑1 (MQ=60)

                                                                      GCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCG                        <  SRR4302534.5144971/94‑1 (MQ=60)

                                                                      GCGATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCG                        <  SRR4302534.1795662/94‑1 (MQ=60)

                                                                        GATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTC                      >  SRR4302534.1565944/1‑94 (MQ=60)

                                                                        GATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTC                      >  SRR4302534.1002792/1‑94 (MQ=60)

                                                                        GATTAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTC                      <  SRR4302534.2504208/94‑1 (MQ=60)

                                                                           TAATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTCACT                   <  SRR4302534.3304424/94‑1 (MQ=60)

                                                                            AATCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTCACTG                  >  SRR4302534.525445/1‑94 (MQ=60)

                                                                              TCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTCACTGGC                <  SRR4302534.3789797/94‑1 (MQ=60)

                                                                              TCAATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTCACTGGC                <  SRR4302534.5306801/94‑1 (MQ=60)

                                                                                AATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTCACTGGCGC              >  SRR4302534.2569425/1‑94 (MQ=60)

                                                                                AATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTCACTGGCGC              >  SRR4302534.4889145/1‑94 (MQ=60)

                                                                                 ATCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTCACTGGCGCA             >  SRR4302534.5640021/1‑94 (MQ=60)

                                                                                  TCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTCANTGGCGCAG            >  SRR4302534.4000683/1‑94 (MQ=60)

                                                                                  TCGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTCACTGGCGCAG            >  SRR4302534.3360237/1‑94 (MQ=60)

                                                                                   CGCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTCACTGGCGCAGA           <  SRR4302534.2035456/94‑1 (MQ=60)

                                                                                    GCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTCACTGGCGCAGAC          >  SRR4302534.4962178/1‑94 (MQ=60)

                                                                                    GCCCCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTCACTGGCGCAGAC          <  SRR4302534.4756129/94‑1 (MQ=60)

                                                                                       CCATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTCACTGGCGCAGACAGG       <  SRR4302534.2968841/94‑1 (MQ=60)

                                                                                        CATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTCACTGGCGCAGACAGGG      <  SRR4302534.3552471/94‑1 (MQ=60)

                                                                                        CATGCAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGGTAAAAAATGCTTCTCCGTGTTCGTCACTGGCGCAGACAGGG      >  SRR4302534.3605278/1‑94 (MQ=60)

                                                                                            CAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTCACTGGCGCAGACAGGGAACA  <  SRR4302534.2979880/94‑1 (MQ=60)

                                                                                            CAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTCACTGGCGCAGACAGGGAACA  >  SRR4302534.5638758/1‑94 (MQ=60)

                                                                                            CAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTCACTGGCGCAGACAGGGAACA  <  SRR4302534.1852412/94‑1 (MQ=60)

                                                                                            CAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTCACTGGCGCAGACAGGGAACA  >  SRR4302534.1776859/1‑94 (MQ=60)

GCTTTAAAAGCTCGCTCATCGAAGAGACAAAAAAGGTGCTGAAACTTAACTCACTGTGGCGCGTCAGGGTGCGATTAATCAATCGCCCCATGTAGGAGTTGCGGCTGTAATTCAGTAACGGAAAGTGTGGCTGCGCGAGGTTAAAAAGTGCTTCTCCGTGTTCGTCACTGGCGCAGACAGGGAACA  >  NC_000913/4555153‑4555338

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 7 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`