Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F2 I207 R1
|
218 |
45.0 |
2482936 |
97.1% |
2410930 |
86.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NZ_CP009273 |
818,875 |
A→G |
N107N (AAT→AAC) |
clsB ← |
cardiolipin synthase ClsB |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NZ_CP009273 | 818,875 | 0 | A | G | 100.0%
| 111.8
/ NA
| 33 | N107N (AAT→AAC) | clsB | cardiolipin synthase ClsB |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (15/18); total (15/18) |
TATGCTCGGCGGAGTAATTCAGCCCGCCAATAAAGGCTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACATTGGTGCGCATACCAAAAAGGCGAGGGCGGGGATCGTAGTAGCGGAACACTACGCCAGCTGCCGTCAGTTCATTGACAAACTCAT > NZ_CP009273/818790‑818960
|
tatGCTCGGCGGAGTAATTCAGCCCGCCAATAAAGGCTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACGTTgg > 2:542684/1‑90 (MQ=255)
tGCTCGGCGGAGTAATTCAGCCCGCCAATAAAGGCTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACGTTGGTg < 2:450299/90‑1 (MQ=255)
cggcggAGTAATTCAGCCCGCCAATAAAGGCTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACGTTGGTGCGCa > 1:904263/1‑90 (MQ=255)
gggggAGTAATTCAGCCCGCCAATAAAGGCTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACGTTGGTGCGCAt < 1:372525/87‑1 (MQ=255)
ggcggAGTAATTCAGCCCGCCAATAAAGGCTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACGTTGGTGCGCAt < 2:694337/90‑1 (MQ=255)
gCCCGCCAATAAAGGCTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACGTTGGTGCGCATACCAAAAAGGCGAg > 1:1009723/1‑90 (MQ=255)
ccGCCAATAAAGGCTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACGTTGGTGCGCATACCAAAAAGGCGAggg > 1:727669/1‑90 (MQ=255)
cGCCAATAAAGGCTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACGTTGGTGCGCATACCAAAAAGGCGAGGGc < 2:1002784/90‑1 (MQ=255)
gCCAATAAAGGCTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACGTTGGTGCGCATACCAAAAAGGCGAGGGCg > 2:816141/1‑90 (MQ=255)
ccAATAAAGGCTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACGTTGGTGCGCATCCCAAAAAGGCGAGGGCgg < 2:584779/90‑1 (MQ=255)
ccAATAAAGGCTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACGTTGGTGCGCATACCAAAAAGGCGAGGGCgg < 1:1138401/90‑1 (MQ=255)
ccAATAAAGGCTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACGTTGGTGCGCATACCAAAAAGGCGAGGGCgg < 2:924723/90‑1 (MQ=255)
aaTAAAGGCTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACGTTGGTGCGCATACCAAAAAGGCGAGGGCgggg < 1:952245/90‑1 (MQ=255)
aaaGGCTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACGTTGGTGCGCATACCAAAAAGGCGAGGGCGGGGATc < 2:808260/90‑1 (MQ=255)
cTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACGTTGGTGCGCATACCAAAAAGGCGAGGGCgg < 1:532638/80‑1 (MQ=255)
cTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACGTTGGTGCGCATACCAAAAAGGCGAGGGCgg > 2:532638/1‑80 (MQ=255)
cTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACGTTGGTGCGCATACCAAAAAGGCGAGGGCGGGGATCgtagt < 2:1009723/90‑1 (MQ=255)
cTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACGTTGGTGCGCATACCAAAAAGGCGAGGGCGGGGATCgtagt > 2:323157/1‑90 (MQ=255)
cTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACGTTGGTGCGCATACCAAAAAGGCGAGGGCGGGGATCgtagt > 1:309392/1‑90 (MQ=255)
gcgcgTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACGTTGGTGCGCATACCAAAAAGGCGAGGGCGGGGATCGTAGTAGCGGa < 1:142569/90‑1 (MQ=255)
cgcgTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACGTTGGTGCGCATACCAAAAAGGCGAGGGCGGGGATCGTAGTAGCGGaa > 2:633306/1‑90 (MQ=255)
gcgTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACGTTGGTGCGCATACCAAAAAGGCGAGGGCGGGGATCGTAGTAGCGGAac > 1:1222530/1‑90 (MQ=255)
gcgTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACGTTGGTGCGCATACCAAAAAGGCGAGGGCGGGGATCGTAGTAGCGGAac > 2:434680/1‑90 (MQ=255)
gcgTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACGTTGGTGCGCATACCAAAAAGGCGAGGGCGGGGATCGTAGTAGCGGAac > 1:642266/1‑90 (MQ=255)
gTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACGTTGGTGCGCATACCAAAAAGGCGAGGGCGGGGATCGTAGTAGCGGAacac > 2:921021/1‑90 (MQ=255)
cGATCACCACAATTTTGCGATGCATCCGGCGAAACACGTTGGTGCGCATACCAAAAAGGCGAGGGCGGGGATCGTAGTAGCGGAACACTa < 2:441037/90‑1 (MQ=255)
cacaATTTTGCGATGCATCCGGCGAAACACGTTGGTGCGCATACCAAAAAGGCGAGGGCGGGGATCGTAGTAGCGGAACACTACGCCAGc < 2:503042/90‑1 (MQ=255)
cacaATTTTGCGATGCATCCGGCGAAACACGTTGGTGCGCATACCAAAAAGGCGAGGGCGGGGATCGTAGTAGCGGAACACTACGCCAGc < 1:371389/90‑1 (MQ=255)
ttGCGATGCATCCGGCGAAACACGTTGGTGCGCATACCAAAAAGGCGAGGGCGGGGATCGTAGTAGCGGAACACTACGCCAGCTGCCGTc > 1:225978/1‑90 (MQ=255)
gCGATGCATCCGGCGAAACACGTTGGTGCGCATACCAAAAAGGCGAGGGCGGGGATCGTAGTAGCGGAACACTACGCCAGCTGCCGTCAg > 1:799707/1‑90 (MQ=255)
aTGCATCCGGCGAAACACGTTGGTGCGCATACCAAAAAGGCGAGGGCGGGGATCGTAGTAGCGGAACACTACGCCAGCTGCCGTCAGTTc < 1:863331/90‑1 (MQ=255)
aaaCACGTTGGTGCGCATACCAAAAAGGCGAGGGCGGGGATCGTAGTAGCGGAACACTACGCCAGCTGCCGTCAGTTCATTGACAAACTc < 2:784159/90‑1 (MQ=255)
acacGTTGGTGCGCATACCAAAAAGGCGAGGGCGGGGATCGTAGTAGCGGAACACTACGCCAGCTGCCGTCAGTTCATTGACAAACTCAt < 2:904263/90‑1 (MQ=255)
|
TATGCTCGGCGGAGTAATTCAGCCCGCCAATAAAGGCTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACATTGGTGCGCATACCAAAAAGGCGAGGGCGGGGATCGTAGTAGCGGAACACTACGCCAGCTGCCGTCAGTTCATTGACAAACTCAT > NZ_CP009273/818790‑818960
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
CCGTAGCTGGACATATGCTCGGCGGAGTAATTCAGCCCGCCAATAAAGGCTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACATTGGTGCGCATACCAAAAAGGCGAGGGCGGGGATCGTAGTAGCGGAACACTACGCCAGCTGCCGTCAGTTCATTGACAAACTCATCGCTGAGATCCGG > NZ_CP009273/818777‑818973
|
CCGTAGCTGGACATATGCTCGGCGGAGTAATTCAGCCCGCCAATAAAGGCTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACGT > SRR3722089.600820/1‑100 (MQ=60)
GACATATGCTCGGCGGAGTAATTCAGCCCGCCAATAAAGGCTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACGTTGGTGCGCA > SRR3722089.917397/1‑100 (MQ=60)
nnnnnnnnnnnnnnnnnnnnCAATAAAGGCTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACGTTGGTGCGCATACCAANNNNG < SRR3722089.1067746/80‑1 (MQ=60)
GGGGGAGTAATTCAGCCCGCCAATAAAGGCTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACGTTGGTGCGCATACCAAAAAGG < SRR3722089.377094/100‑1 (MQ=60)
GAGTAATTCAGCCCGCCAATAAAGGCTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACGTTGGTGCGCATACCAAAAAGGCGAG > SRR3722089.1024763/1‑100 (MQ=60)
GTAATTCAGCCCGCCAATAAAGGCTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACGTTGGTGCGCATACCAAAAAGGCGAGGG > SRR3722089.737132/1‑100 (MQ=60)
CCAATAAAGGCTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACGTTGGTGCGCATACCAAAAAGGCGAGGGCGGGGATCGTAGT > SRR3722089.313309/1‑100 (MQ=60)
CCAATAAAGGCTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACGTTGGTGCGCATACCAAAAAGGCGAGGGCGGGGATCGTAGT < SRR3722089.538847/100‑1 (MQ=60)
CCAATAAAGGCTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACGTTGGTGCGCATACCAAAAAGGCGAGGGCGGGGATCGTAGT < SRR3722089.1155720/100‑1 (MQ=60)
AATAAAGGCTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACGTTGGTGCGCATACCAAAAAGGCGAGGGCGGGGATCGTAGTAG < SRR3722089.966232/100‑1 (MQ=60)
GGCTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACGTTGGTGCGCATACCAAAAAGGCGAGGGCGGGGATCGTAGTAGCGGAAC > SRR3722089.1241107/1‑100 (MQ=60)
GGCTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACGTTGGTGCGCATACCAAAAAGGCGAGGGCGGGGATCGTAGTAGCGGAAC > SRR3722089.650473/1‑100 (MQ=60)
GCGCGTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACGTTGGTGCGCATACCAAAAAGGCGAGGGCGGGGATCGTAGTAGCGGAACACTACGCC < SRR3722089.144320/100‑1 (MQ=60)
CACCACAATTTTGCGATGCATCCGGCGAAACACGTTGGTGCGCATACCAAAAAGGCGAGGGCGGGGATCGTAGTAGCGGAACACTACGCCAGCTGCCGTC > SRR3722089.228853/1‑100 (MQ=60)
CCACAATTTTGCGATGCATCCGGCGAAACACGTTGGTGCGCATACCAAAAAGGCGAGGGCGGGGATCGTAGTAGCGGAACACTACGCCAGCTGCCGTCAG > SRR3722089.810759/1‑100 (MQ=60)
CACAATTTTGCGATGCATCCGGCGAAACACGTTGGTGCGCATACCAAAAAGGCGAGGGCGGGGATCGTAGTAGCGGAACACTACGCCAGCTGCCGTCAGT < SRR3722089.375947/100‑1 (MQ=60)
ATGCATCCGGCGAAACACGTTGGTGCGCATACCAAAAAGGCGAGGGCGGGGATCGTAGTAGCGGAACACTACGCCAGCTGCCGTCAGTTCATTGACAAAC < SRR3722089.875684/100‑1 (MQ=60)
GCGAAACACGTTGGTGCGCATACCAAAAAGGCGAGGGCGGGGATCGTAGTAGCGGAACACTACGCCAGCTGCCGTCAGTTCATTGACAAACTCATCGCTG > SRR3722089.1021907/1‑100 (MQ=60)
AAACACGTTGGTGCGCATACCAAAAAGGCGAGGGCGGGGATCGTAGTAGCGGAACACTACGCCAGCTGCCGTCAGTTCATTGACAAACTCATCGCTGAGA > SRR3722089.712059/1‑100 (MQ=60)
ACGTTGGTGCGCATACCAAAAAGGCGAGGGCGGGGATCGTAGTAGCGGAACACTACGCCAGCTGCCGTCAGTTCATTGACAAACTGATCGCTGAGATCCG > SRR3722089.396813/1‑100 (MQ=60)
CGTTGGTGCGCATACCAAAAAGGCGAGGGCGGGGATCGTAGTAGCGGAACACTACGCCAGCTGCCGTCAGTTCATTGACAAACTCATCGCTGAGATCCGG < SRR3722089.368652/100‑1 (MQ=60)
|
CCGTAGCTGGACATATGCTCGGCGGAGTAATTCAGCCCGCCAATAAAGGCTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACATTGGTGCGCATACCAAAAAGGCGAGGGCGGGGATCGTAGTAGCGGAACACTACGCCAGCTGCCGTCAGTTCATTGACAAACTCATCGCTGAGATCCGG > NZ_CP009273/818777‑818973
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
