Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F2 I225 R1
|
227 |
21.4 |
1179702 |
97.1% |
1145490 |
86.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NZ_CP009273 |
818,875 |
A→G |
N107N (AAT→AAC) |
clsB ← |
cardiolipin synthase ClsB |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NZ_CP009273 | 818,875 | 0 | A | G | 100.0%
| 41.4
/ NA
| 14 | N107N (AAT→AAC) | clsB | cardiolipin synthase ClsB |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (8/6); total (8/6) |
TATGCTCGGCGGAGTAATTCAGCCCGCCAATAAAGGCTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACATTGGTGCGCATACCAAAAAGGCGAGGGCGGGGATCGTAGTAGCGGAACACTACGCCAGCTGCCGTCAGTTCATTGACAAACTCAT > NZ_CP009273/818790‑818960
|
tatGCTCGGCGGAGTAATTCAGCCCGCCAATAAAGGCTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACGTTgg > 1:17922/1‑90 (MQ=255)
tatGCTCGGCGGAGTAATTCAGCCCGCCAATAAAGGCTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACGTTgg > 1:503893/1‑90 (MQ=255)
tCGGCGGAGTAATTCAGCCCGCCAATAAAGGCTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACGTTGGTgcgc > 2:458079/1‑90 (MQ=255)
ggcggATTATTTCAGCCCGCCAATAAAGGCTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACGTTGGTGCGCAt < 1:72169/90‑1 (MQ=255)
gCCCGCCAATAAAGGCTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACGTTGGTGCGCATACCAAAAAGGCGAg > 1:67533/1‑90 (MQ=255)
gCCCGCCAATAAAGGCTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACGTTGGTGCGCATACCAAAAAGGCGAg > 2:588875/1‑90 (MQ=255)
gCCAATAAAGGCTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACGTTGGTGCGCATACCAAAAAGGCGAGGGCg > 1:373279/1‑90 (MQ=255)
aaTAAAGGCTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACGTTGGTGCGCATACCAAAAAGGCGAGGGCgggg < 2:303134/90‑1 (MQ=255)
gcgTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACGTTGGTGCGCATACCAAAAAGGCGAGGGCGGGGCTCGTAGTAGGGGCac > 1:464840/1‑90 (MQ=255)
gcgTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACGTTGGTGCGCATACCAAAAAGGCGAGGGCGGGGATCGTAGTAGCGGAac > 1:83958/1‑90 (MQ=255)
gcgTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACGTTGGTGCGCATACCAAAAAGGCGAGGGCGGGGATCGTAGTAGCGGAac < 2:206051/90‑1 (MQ=255)
cacaATTTTGCGATGCATCCGGCGAAACACGTTGGTGCGCATACCAAAAAGGCGAGGGCGGGGATCGTAGTAGCGGAACACTACGCCAGc < 1:241784/90‑1 (MQ=255)
gCATCCGGCGAAACACGTTGGTGCGCATACCAAAAAGGCGAGGGCGGGGATCGTAGTAGCGGAACACTACGCCAGCTGCCGTCAGTTCAt < 2:83958/90‑1 (MQ=255)
acacGTTGGTGCGCATACCAAAAAGGCGAGGGCGGGGATCGTAGTAGCGGAACACTACGCCAGCTGCCGTCAGTTCATTGACAAACTCAt < 1:494524/90‑1 (MQ=255)
|
TATGCTCGGCGGAGTAATTCAGCCCGCCAATAAAGGCTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACATTGGTGCGCATACCAAAAAGGCGAGGGCGGGGATCGTAGTAGCGGAACACTACGCCAGCTGCCGTCAGTTCATTGACAAACTCAT > NZ_CP009273/818790‑818960
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
TAGCTGGACATATGCTCGGCGGAGTAATTCAGCCCGCCAATAAAGGCTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACATTGGTGCGCATACCAAAAAGGCGAGGGCGGGGATCGTAGTAGCGGAACACTACGCCAGCTGCCGTCAGTTCATTGACAAACTCATCGCTGAGATCCG > NZ_CP009273/818780‑818972
|
TAGCTGGACATATGCTCGGCGGAGTAATTCAGCCCGCCAATAAAGGCTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACGTTGG > SRR3722109.18156/1‑100 (MQ=60)
TAGCTGGACATATGCTCGGCGGAGTAATTCAGCCCGCCAATAAAGGCTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACGTTGG > SRR3722109.511512/1‑100 (MQ=60)
GGCGGATTATTTCAGCCCGCCAATAAAGGCTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACGTTGGTGCGCATACCAAAAAGG < SRR3722109.73040/100‑1 (MQ=60)
GAATAATTCAGCCCGCCAATAAAGGCTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACGTTGGTGCGCATACCAAAAAGGCGAG > SRR3722109.68338/1‑100 (MQ=60)
AATTCAGCCCGCCAATAAAGGCTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACGTTGGTGCGCATACCAAAAAGGCGAGGGCG > SRR3722109.378543/1‑100 (MQ=60)
GGCTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACGTTGGTGCGCATACCAAAAAGGCGAGGGCGGGGCTCGTAGTAGGGGCAC > SRR3722109.471828/1‑100 (MQ=60)
GGCTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACGTTGGTGCGCATACCAAAAAGGCGAGGGCGGGGATCGTAGTAGCGGAAC > SRR3722109.84990/1‑100 (MQ=60)
CACAATTTTGCGATGCATCCGGCGAAACACGTTGGTGCGCATACCAAAAAGGCGAGGGCGGGGATCGTAGTAGCGGAACACTACGCCAGCTGCCGTCAGT < SRR3722109.244628/100‑1 (MQ=60)
GAACACGTTGGGGCGCATACCAAAAAGGCGAGGGCGGGGGTCGTAGTAGCGGAACACTACGCGAGCTGCAGGGAGTTCATTGTCAAACTCATCGCTGAGG > SRR3722109.316423/1‑100 (MQ=60)
ACACGTTGGTGCGCATACCAAAAAGGCGAGGGCGGGGATCGTAGTAGCGGAACACTACGCCAGCTGCCGTCAGTTCATTGACAAACTCATCGCTGAGATC < SRR3722109.501990/100‑1 (MQ=60)
ACACGTTGGTGCGCATACCAAAAAGGCGAGGGCGGGGATCGTAGTAGCGGAACACTACGCCAGCTGCCGTCAGTTCATTGACAAACTCATCGCTGAGATC > SRR3722109.98886/1‑100 (MQ=60)
ACGTTGGTGCGCATACCAAAAAGGCGAGGGCGGGGATCGTAGTAGCGGAACACTACGCCAGCTGCCGTCAGTTCATTGACAAACTCATCGCTGAGATCCG > SRR3722109.103336/1‑100 (MQ=60)
|
TAGCTGGACATATGCTCGGCGGAGTAATTCAGCCCGCCAATAAAGGCTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACATTGGTGCGCATACCAAAAAGGCGAGGGCGGGGATCGTAGTAGCGGAACACTACGCCAGCTGCCGTCAGTTCATTGACAAACTCATCGCTGAGATCCG > NZ_CP009273/818780‑818972
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
