Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F2 I207 R1
|
218 |
45.0 |
2482936 |
97.1% |
2410930 |
86.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NZ_CP009273 |
979,260 |
T→C |
I52T (ATC→ACC) |
gloC → |
hydroxyacylglutathione hydrolase GloC |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NZ_CP009273 | 979,260 | 0 | T | C | 100.0%
| 86.5
/ NA
| 26 | I52T (ATC→ACC) | gloC | hydroxyacylglutathione hydrolase GloC |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (12/14); total (12/14) |
CCCGTCTGGCCGCACTGGTCGATCCTGGCGGCGATGCGGAAAAAATCAAACAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGATCCTGCTGACGCATGGTCATCTGGACCACGTTGGCGCAGCGGCGGAACTGGCGCAACATTACGGCGTGCCGGTTTTCGGCCC > NZ_CP009273/979173‑979341
|
cccGTCTGGCCGCACTGGTCGATCCTGGCGGCGATGCGGAAAAAATCAAACAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGAccc < 2:245804/90‑1 (MQ=255)
gTCTGGCCGCACTGGTCGATCCTGGCGGCGATGCGGAAAAAATCAAACAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGACCctgc > 2:844667/1‑90 (MQ=255)
gTCTGGCCGCACTGGTCGATCCTGGCGGCGATGCGGAAAAAATCAAACAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGACCctgc > 1:1069262/1‑90 (MQ=255)
gTCTGGCCGCACTGGTCGATCCTGGCGGCGATGCGGAAAAAATCAAACAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGACCctgc > 1:597/1‑90 (MQ=255)
gTCTGGCCGCACTGGTCGATCCTGGCGGCGATGCGGAAAAAATCAAACAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGACCctgc < 1:667685/90‑1 (MQ=255)
gTCTGGCCGCACTGGTCGATCCTGGCGGCGATGCGGAAAAAATCAAACAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGACCctgc > 1:952606/1‑90 (MQ=255)
gCACTGGTCGATCCTGGCGGCGATGCGGAAAAAATCAAACAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGACCCTGCTGACGCAt < 1:1193287/90‑1 (MQ=255)
gCACTGGTCGATCCTGGCGGCGATGCGGAAAAAATCAAACAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGACCCTGCTGACGCAt < 1:777323/90‑1 (MQ=255)
aCTGGTCGATCCTGGCGGCGATGCGGAAAAAATCAAACAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGACCCTGCTGACGCATgg < 2:1137934/90‑1 (MQ=255)
aCTGGTCGATCCTGGCGGCGATGCGGAAAAAATCAAACAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGACCCTGCTGACGCATgg < 2:1069262/90‑1 (MQ=255)
aTCCTGGCGGCGATGCGGAAAAAATCAAACAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGACCCTGCTGACGCATGGTCATCTgg > 1:725134/1‑90 (MQ=255)
tCCTGGCGGCGATGCGGAAAAAATCAAACAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGACCCTGCTGACGCATGGTCATCTGGa < 2:781695/90‑1 (MQ=255)
cTGGCGGCGATGCGGAAAAAATCAAACAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGACCCTGCTGACGCATGGTCATCTGGAcc < 1:558455/90‑1 (MQ=255)
ggcggcGATGCGGAAAAAATCAAACAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGACCCTGCTGACGCATGGTCATCTGGACCAc > 1:1084942/1‑90 (MQ=255)
cgATGCGGAAAAAATCAAACAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGACCCTGCTGACGCATGGTCATCTGGACCACGTTgg > 1:769625/1‑90 (MQ=255)
cgATGCGGAAAAAATCAAACAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGACCCTGCTGACGCATGGTCATCTGGACCACGTTgg > 2:1170986/1‑90 (MQ=255)
aaaaaTCAAACAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGACCCTGCTGACGCATGGTCATCTGGACCACGTTGGCGCAgcggc < 1:355395/90‑1 (MQ=255)
aaaaTCAAACAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGACCCTGCTGACGCATGGTCATCTGGACCACGTTGGCGCAgcggcg < 1:998790/90‑1 (MQ=255)
aCAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGACCCTGCTGACGCATGGTCATCTGGACCACGTTGGCGCAGCGGCGGAACTGgc < 2:1084942/90‑1 (MQ=255)
aGGAAGTTGATGACAGCGGCCTGACACTGATGCAGACCCTGCTGACGCATGGTCATCTGGACCACGTTGGCGCAGCGGCGGAACTGgcgc < 2:1059681/90‑1 (MQ=255)
aGGAAGTTGATGACAGCGGCCTGACACTGATGCAGACCCTGCTGACGCATGGTCATCTGGACCACGTTGGCGCAGCGGCGGAACTGgcgc < 2:769625/90‑1 (MQ=255)
aGGAAGTTGATGACAGCGGCCTGACACTGATGCAGACCCTGCTGACGCATGGTCATCTGGACCACGTTGGCGCAGCGGCGGAACTGgcgc < 1:1186249/90‑1 (MQ=255)
gCGGCCTGACACTGATGCAGACCCTGCTGACGCATGGTCATCTGGACCACGTTGGCGCAGCGGCGGAACTGGCGCAACATTACGGCGTGc > 2:857768/1‑90 (MQ=255)
gATGCAGACCCTGCTGACGCATGGTCATCTGGACCACGTTGGCGCAGCGGCGGAACTGgcgc > 2:538710/1‑62 (MQ=255)
gATGCAGACCCTGCTGACGCATGGTCATCTGGACCACGTTGGCGCAGCGGCGGAACTGgcgc < 1:538710/62‑1 (MQ=255)
gATGCAGACCCTGCTGACGCATGGTCATCTGGACCACGTTGGCGCAGCGGCGGAACTGGCGCAACATTACGGCGTGCCGGTTTTCGGccc > 2:1203543/1‑90 (MQ=255)
gATGCAGACCCTGCTGACGCATGGTCATCTGGACCACGTTGGCGCAGCGGCGGAACTGGCGCAACATTACGGCGTGCCGGGTTTCGGccc > 2:985197/1‑90 (MQ=255)
|
CCCGTCTGGCCGCACTGGTCGATCCTGGCGGCGATGCGGAAAAAATCAAACAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGATCCTGCTGACGCATGGTCATCTGGACCACGTTGGCGCAGCGGCGGAACTGGCGCAACATTACGGCGTGCCGGTTTTCGGCCC > NZ_CP009273/979173‑979341
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
GGTGTGAACAAACCCGTCTGGCCGCACTGGTCGATCCTGGCGGCGATGCGGAAAAAATCAAACAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGATCCTGCTGACGCATGGTCATCTGGACCACGTTGGCGCAGCGGCGGAACTGGCGCAACATTACGGCGT‑GCCGGTTTTCGGCCCGGAAAAA > NZ_CP009273/979161‑979348
|
GGTGTGAACAAACCCGTCTGGCCGCACTGGTCGATCCTGGCGGCGATGCGGAAAAAATCAAACAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGAC > SRR3722089.1103730/1‑100 (MQ=60)
GGTGTGAACAAACCCGTCTGGCCGCACTGGTCGATCCTGGCGGCGATGCGGAAAAAATCAAACAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGAC > SRR3722089.966013/1‑100 (MQ=60)
GAACAAACCCGTCTGGCCGCACTGGTCGATCCTGGCGGCGATGCGGAAAAAATCAAACAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGACCCTGC > SRR3722089.1085315/1‑100 (MQ=60)
GAACAAACCCGTCTGGCCGCACTGGTCGATCCTGGCGGCGATGCGGAAAAAATCAAACAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGACCCTGC > SRR3722089.966602/1‑100 (MQ=60)
GAACAAACCCGTCTGGCCGCACTGGTCGATCCTGGCGGCGATGCGGAAAAAATCAAACAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGACCCTGC > SRR3722089.602/1‑100 (MQ=60)
CAAACCCGTCTGGCCGCACTGGTCGATCCTGGCGGCGATGCGGAAAAAATCAAACAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGACCCTGCTGA < SRR3722089.61245/100‑1 (MQ=60)
CAAACCCGTCTGGCCGCACTGGTCGATCCTGGCGGCGATGCGGAAAAAATCAAACAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGACCCTGCTGA < SRR3722089.263121/100‑1 (MQ=60)
AACCCGTCTGGCCGCACTGGTCGATCCTGGCGGCGATGCGGAAAAAATCAAACAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGACCCTGCTGACG < SRR3722089.996129/100‑1 (MQ=60)
AACCCGTCTGGCCGCACTGGTCGATCCTGGCGGCGATGCGGAAAAAATCAAACAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGACCCTGCTGACG < SRR3722089.891494/100‑1 (MQ=60)
GTCTGGCCGCACTGGTCGATCCTGGCGGCGATGCGGAAAAAATCAAACAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGACCCTGCTGACGCATGG < SRR3722089.676322/100‑1 (MQ=60)
GCACTGGTCGATCCTGGCGGCGATGCGGAAAAAATCAAACAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGACCCTGCTGACGCATGGTCATCTGG < SRR3722089.1211429/100‑1 (MQ=60)
GCACTGGTCGATCCTGGCGGCGATGCGGAAAAAATCAAACAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGACCCTGCTGACGCATGGTCATCTGG > SRR3722089.734572/1‑100 (MQ=60)
GCACTGGTCGATCCTGGCGGCGATGCGGAAAAAATCAAACAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGACCCTGCTGACGCATGGTCATCTGG < SRR3722089.787885/100‑1 (MQ=60)
GGTCGATCCTGGCGGCGATGCGGAAAAAATCAAACAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGACCCTGCTGACGCActgtctcttatacaca > SRR3722089.319822/1‑84 (MQ=60)
GGTCGATCCTGGCGGCGATGCGGAAAAAATCAAACAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGACCCTGCTGACGCATGGTCATCTGGACCAC > SRR3722089.1101284/1‑100 (MQ=60)
ATCCTGGCGGCGATGCGGAAAAAATCAAACAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGACCCTGCTGACGCATGGTCATCTGGACCACGTTGG > SRR3722089.780007/1‑100 (MQ=60)
CTGGCGGCGATGCGGAAAAAATCAAACAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGACCCTGCTGACGCATGGTCATCTGGACCACGTTGGCGC < SRR3722089.564949/100‑1 (MQ=60)
AAAAATCAAACAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGACCCTGCTGACGCATGGTCATCTGGACCACGTTGGCGCAGCGGCGGAACTGGCG < SRR3722089.359783/100‑1 (MQ=60)
AAAATCAAACAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGACCCTGCTGACGCATGGTCATCTGGACCACGTTGGCGCAGCGGCGGAACTGGCGC < SRR3722089.1013639/100‑1 (MQ=60)
gatgtgtataagagacaGGCCTGACACTGATGCAGACCCTGCTGACGCATGGTCATCTGGACCACGTTGGCGCAGCGGCGGAACTGGCGCAACATTACGG < SRR3722089.544982/83‑1 (MQ=60)
AGGAAGTTGATGACAGCGGCCTGACACTGATGCAGACCCTGCTGACGCATGGTCATCTGGACCACGTTGGCGCAGCGGCGGAACTGGCGCAACATTACGG < SRR3722089.1204306/100‑1 (MQ=60)
CACTGATGCAGACCCTGCTGACGCATGGTCATCTGGACCACGTTGGCGCAGCGGCGGAACTGGCGCAACATTACGGCGT‑GCCGGTTTTCGGCCCGGGAAA > SRR3722089.117147/1‑100 (MQ=60)
CTGATGCAGACCCTGCTGACGCATGGTCATCTGGACCACGTTGGCGCAGCGGCGGGACTGGCGCAACATTTCGGCGTGGCGGGTTTTTGGCCCGGAAAAA > SRR3722089.735801/1‑100 (MQ=60)
|
GGTGTGAACAAACCCGTCTGGCCGCACTGGTCGATCCTGGCGGCGATGCGGAAAAAATCAAACAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGATCCTGCTGACGCATGGTCATCTGGACCACGTTGGCGCAGCGGCGGAACTGGCGCAACATTACGGCGT‑GCCGGTTTTCGGCCCGGAAAAA > NZ_CP009273/979161‑979348
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
