Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F2 I199 R1
|
220 |
57.5 |
3163680 |
97.0% |
3068769 |
86.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NZ_CP009273 |
979,260 |
T→C |
I52T (ATC→ACC) |
gloC → |
hydroxyacylglutathione hydrolase GloC |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NZ_CP009273 | 979,260 | 0 | T | C | 100.0%
| 76.8
/ NA
| 25 | I52T (ATC→ACC) | gloC | hydroxyacylglutathione hydrolase GloC |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (13/12); total (13/12) |
GCCGCACTGGTCGATCCTGGCGGCGATGCGGAAAAAATCAAACAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGATCCTGCTGACGCATGGTCATCTGGACCACGTTGGCGCAGCGGCGGAACTGGCGCAACATTACGGCGTGCCGGTTTTCGGCCC > NZ_CP009273/979181‑979341
|
gCCGCACTGGTCGATCCTGGCGGCGATGCGGAAAAAATCAAACAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGACCctgc < 2:1003578/85‑1 (MQ=255)
gCCGCACTGGTCGATCCTGGCGGCGATGCGGAAAAAATCAAACAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGACCctgc > 1:1003578/1‑85 (MQ=255)
cACTGGTCGATCCTGGCGGCGATGCGGAAAAAATCAAACAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGACCCTGCTGACGCATg > 1:1102330/1‑90 (MQ=255)
aCTGGTCGATCCTGGCGGCGATGCGGAAAAAATCAAACAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGACCCTGCTGACGCATgg < 1:944391/90‑1 (MQ=255)
ggTCGATCCTGGCGGCGATGCGGAAAAAATCAAACAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGACCCTGCTGACGCATGGTCa < 2:1565861/90‑1 (MQ=255)
ggTCGATCCTGGCGGCGATGCGGAAAAAATCAAACAGGAAGGTGATGACAGCGGCCTGACACTGATCCAGACCCTGCTGACGCATGGTCa > 1:1446672/1‑90 (MQ=255)
cGATCCTGGCGGCGATGCGGAAAAAATCAAACAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGACCCTGCTGACGCATGGTCATCt < 2:1215245/90‑1 (MQ=255)
cTGGCGGCGATGCGGAAAAAATCAAACAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGACCCTGCTGACGCATGGTCATCTGGAcc < 1:957719/90‑1 (MQ=255)
ggcggcGATGCGGAAAAAATCAAACAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGACCCTGCTGACGCATGGTCATCTGGACCAc > 1:1439181/1‑90 (MQ=255)
ggcggcGATGCGGAAAAAATCAAACAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGACCCTGCTGACGCATGGTCATCTGGACCAc > 2:432217/1‑90 (MQ=255)
ggcgATGCGGAAAAAATCAAACAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGACCCTGCTGACGCATGGTCATCTGGACCACGtt < 1:965194/90‑1 (MQ=255)
cgATGCGGAAAAAATCAAACAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGACCCTGCTGACGCATGGTCATCTGGACCACGTTgg > 1:722563/1‑90 (MQ=255)
aaaaaTCAAACAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGACCCTGCTGACGCATGGTCATCTGGACCACGTTGGCGCAgcggc < 1:543101/90‑1 (MQ=255)
aCAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGACCCTGCTGACGCATGGTCATCTGGACCACGTTGGCGCAGCGGCGGAACTGgc < 1:758141/90‑1 (MQ=255)
aGGAAGTTGATGACAGCGGCCTGACACTGATGCAGACCCTGCTGACGCATGGTCATCTGGACCACGTTGGCGCAGCGGCGGAACTGgcgc < 1:89222/90‑1 (MQ=255)
aGGAAGTTGATGACAGCGGCCTGACACTGATGCAGACCCTGCTGACGCATGGTCATCTGGACCACGTTGGCGCAGCGGCGGAACTGgcgc < 2:1280054/90‑1 (MQ=255)
aGGAAGTTGATGACAGCGGCCTGACACTGATGCAGACCCTGCTGACGCATGGTCATCTGGACCACGTTGGCGCAGCGGCGGAACTGgcgc < 1:536533/90‑1 (MQ=255)
atgaCAGCGGCCTGACACTGATGCAGACCCTGCTGACGCATGGTCATCTGGACCACGTTGGCGCAGCGGCGGAACTGgcgc > 1:53889/1‑81 (MQ=255)
atgaCAGCGGCCTGACACTGATGCAGACCCTGCTGACGCATGGTCATCTGGACCACGTTGGCGCAGCGGCGGAACTGgcgc < 2:53889/81‑1 (MQ=255)
gCGGCCTGACACTGATGCAGACCCTGCTGACGCATGGTCATCTGGACCACGTTGGCGCAGCGGCGGAACTGGCGCAACATTACGGCGTGc > 2:782855/1‑90 (MQ=255)
gATGCAGACCCTGCTGACGCATGGTCATCTGGACCACGTTGGCGCAGCGGCGGAACTGGCGCAACATTACGGCGTGCCGGTTTTCGGccc > 2:1329494/1‑90 (MQ=255)
gATGCAGACCCTGCTGACGCATGGTCATCTGGACCACGTTGGCGCAGCGGCGGAACTGGCGCAACATTACGGCGTGCCGGTTTTCGGccc > 2:285368/1‑90 (MQ=255)
gATGCAGACCCTGCTGACGCATGGTCATCTGGACCACGTTGGCGCAGCGGCGGAACTGGCGCAACATTACGGCGTGCCGGTTTTCGGccc > 2:516009/1‑90 (MQ=255)
gATGCAGACCCTGCTGACGCATGGTCATCTGGACCACGTTGGCGCAGCGGCGGAACTGGCGCAACATTACGGCGTGCCGGGTTTCGGccc > 2:1152480/1‑90 (MQ=255)
gATGCAGACCCTGCTGACGCATGGTCATCTGGACCACGTTGGCGCAGCGGCGGAACTGGCGCAACATTACGGCGGGCCGGGTTTCGGccc > 2:1218782/1‑90 (MQ=255)
|
GCCGCACTGGTCGATCCTGGCGGCGATGCGGAAAAAATCAAACAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGATCCTGCTGACGCATGGTCATCTGGACCACGTTGGCGCAGCGGCGGAACTGGCGCAACATTACGGCGTGCCGGTTTTCGGCCC > NZ_CP009273/979181‑979341
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
GGTGTGAACAAACCCGTCTGGCCGCACTGGTCGATCCTGGCGGCGATGCGGAAAAAATCAAACAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGATCCTGCTGACGCATGGTCATCTGGACCACGTTGGCGCAGCGGCGGAACTGGCGCAACATTACGGCGTGCCGGTTTTCGGCCCGGAAAAAGAAGATGA > NZ_CP009273/979161‑979356
|
GGTGTGAACAAACCCGTCTGGCCGCACTGGTCGATCCTGGCGGCGATGCGGAAAAAATCAAACAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGAC > SRR3722078.999179/1‑100 (MQ=60)
GGTGTGAACAAACCCGTCTGGCCGCACTGGTCGATCCTGGCGGCGATGCGGAAAAAATCAAACAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGAC < SRR3722078.539661/100‑1 (MQ=60)
GGTGTGAACAAACCCGTCTGGCCGCACTGGTCGATCCTGGCGGCGATGCGGAAAAAATCAAACAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGAC < SRR3722078.524870/100‑1 (MQ=60)
AACCCGTCTGGCCGCACTGGTCGATCCTGGCGGCGATGCGGAAAAAATCAAACAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGACCCTGCTGACG > SRR3722078.1018263/1‑100 (MQ=60)
ACCCGTCTGGCCGCACTGGTCGATCCTGGCGGCGATGCGGAAAAAATCAAACAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGACCCTGCTGACGC < SRR3722078.336712/100‑1 (MQ=60)
CGTCTGGCCGCACTGGTCGATCCTGGCGGCGATGCGGAAAAAATCAAACAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGACCCTGCTGACGCATG > SRR3722078.1119126/1‑100 (MQ=60)
TGGCCGCACTGGTCGATCCTGGCGGCGATGCGGAAAAAATCAAACAGGAAGGTGATGACAGCGGCCTGACACTGATCCAGACCCTGCTGACGCATGGTCA > SRR3722078.1470249/1‑100 (MQ=60)
ACTGGTCGATCCTGGCGGCGATGCGGAAAAAATCAAACAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGACCCTGCTGACGCATGGTCATCTGGAC < SRR3722078.957575/100‑1 (MQ=60)
GGTCGATCCTGGCGGCGATGCGGAAAAAATCAAACAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGACCCTGCTGACGCATGGTCATCTGGACCAC > SRR3722078.1462607/1‑100 (MQ=60)
ATCCTGGCGGCGATGCGGAAAAAATCAAACAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGACCCTGCTGACGCATGGTCATCTGGACCACGTTGG > SRR3722078.731534/1‑100 (MQ=60)
CTGGCGGCGATGCGGAAAAAATCAAACAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGACCCTGCTGACGCATGGTCATCTGGACCACGTTGGCGC < SRR3722078.971276/100‑1 (MQ=60)
GGCGATGCGGAAAAAATCAAACAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGACCCTGCTGACGCATGGTCATCTGGACCACGTTGGCGCAGCGG < SRR3722078.978929/100‑1 (MQ=60)
AAAAATCAAACAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGACCCTGCTGACGCATGGTCATCTGGACCACGTTGGCGCAGCGGCGGAACTGGCG < SRR3722078.549519/100‑1 (MQ=60)
ACAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGACCCTGCTGACGCATGGTCATCTGGACCACGTTGGCGCAGCGGCGGAACTGGCGCAACATTAC < SRR3722078.767863/100‑1 (MQ=60)
CAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGACCCTGCTGACGCATGGTCATCTGGACCACGTTGGCGCAGCGGCGGAACTGGCGCAACATTACG > SRR3722078.54529/1‑100 (MQ=60)
tcggcagcgtcagatgtgtataagagacagTGCAGACCCTGCTGACGCATGGTCATCTGGACCACGTTGGCGCAGCGGCGGAACTGGCGCAACATTACGG < SRR3722078.1490043/70‑1 (MQ=60)
AGGAAGTTGATGACAGCGGCCTGACACTGATGCAGACCCTGCTGACGCATGGTCATCTGGACCACGTTGGCGCAGCGGCGGAACTGGCGCAACATTACGG < SRR3722078.542876/100‑1 (MQ=60)
AGGAAGTTGATGACAGCGGCCTGACACTGATGCAGACCCTGCTGACGCATGGTCATCTGGACCACGTTGGCGCAGCGGCGGAACTGGCGCAACATTACGG < SRR3722078.90274/100‑1 (MQ=60)
GATGCAGATCCTGCTGACGCATGGTCATCTGGACCACGTTGGCGCAGCGGCGGAACTGGCGCAACATTACGGCGTGCCGGTTTTCGGCCCGGGAAAAAAA > SRR3722078.1386460/1‑100 (MQ=60)
GATGCAGACCCTGCTGACGCATGGTCATCTGGACCACGTTGGCGCAGCGGCGGAACTGGCGCAACATTACGGCGTGCCGGTTTTCGGCCCCGGAAAAGAA > SRR3722078.744351/1‑100 (MQ=60)
AGATCCTGCTGACGCATGGTCATCTGGACCACGTTGGCGCAGCGGCGGAACTGGCGCAACATTACGGCGTGCCGGTTTTCGGCCCGGGAAAAGAAGATGA > SRR3722078.737441/1‑100 (MQ=60)
|
GGTGTGAACAAACCCGTCTGGCCGCACTGGTCGATCCTGGCGGCGATGCGGAAAAAATCAAACAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGATCCTGCTGACGCATGGTCATCTGGACCACGTTGGCGCAGCGGCGGAACTGGCGCAACATTACGGCGTGCCGGTTTTCGGCCCGGAAAAAGAAGATGA > NZ_CP009273/979161‑979356
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
